MC06g1062 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1062
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionaluminum-activated malate transporter 2-like
LocationMC06: 10476664 .. 10480421 (-)
RNA-Seq ExpressionMC06g1062
SyntenyMC06g1062
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAGAGTAGACGCCATTAGCAAAACCTCATCATACCCCTCTCTCTCTCTCAAATTTTCCAAAAAAGATTTCAGCTACTTCAGCTTATGGAGATGGCTGATCATAAGAGTGAGGGAGTTTCGTCAGCTCGGTGGGCCGAGGGGCTCAGAGCCAAATCCTCGAGACTAACGGTGAAGGTGGGTGAGTTAGCGAGGAAGACAAAGAAGCTGGCCAAGGATGATCCGAGAAGAATCGCTCATTCCCTCAAAGTAGCCTTAGCCATCACTTTGGTTTCATTGTTCTACTACTTCGAACCGCTGTATGATGGCTTTGGTGCCTCCGCTATGTGGGCTATCTTAACAGTTGTAGTCGTCTTCGAGTTCTCTGTAGGTAAATATATTCTGATCTCCCTCGTAATGTGACTGTCACTCTTACGATCGTTGTTGAATAGAATATTATGAAACAGGAGCAACACTTGGACGAGGATTGAACAGAGTTTTGGCAACATTTTCGGCTGCGGCTCTGGGATTTGGAGCCCATTTTTTGGCAGATTTGGCTGCAGACAAAGGCGAACCCATTATTCTTGCTTTCTTTGTTTTCTTACTTGGTACAAATTTCTACTTTATCAATTTTATTTTTTATTTTTTATTTTTTTATTTTTTATTTTTTTTGTCTCCGATAAATTTACACCTATCTCAACTAATATTATAGGACAACTCATTCGACCTTTGTCAAAAAATGTAGAATTTTATTATCCTACGTTGGTGTGGGAACTTTACTTTTTTTTTTTTTTATAACGTCAAATAAAATATATATTTTTCCTTTTAAATACAAATATAACATGTAATAGTAAAATTTTATTCCTACTCATGATTTGTCGTGATAGATAGACTTAAATTTATGAAGGAAGGAAGAGTAAATTATTTAAATTCCAAAATTGGAGAAACTTTACTTATAATCCCTTTTAAGTATCATTTTAGTCATTTTCATTATTTTTCATATATCATTCAATATAATTGATATATTTTTGTAGATATATAAATATTTGAAATCACATGTTGCTAGCGATGATAATCATCGACAAGAACTAAAGTAAGTGGATATATAACTATTTGAAATCACAGGTTGCTAGTGAAAGTGATGATAAATTATCAACAAGAACTAAAGTAATTTTTCATATACAGGTAGTTTAAGGCTCGTAAAAAGTTTAGACAATAAAGAAAAATTTAACCTAATTTTTTTATATATAGTTATTATATAAAACATAGCTTTACAAAAGCAAAACACTTCTTATATTAACCAAAACAACAAAGTCCATATAGTTTATATGGTTTAACCATGTAGGAGATGAGAATTTGAACATCCGGCCTTTAGGATAAATGTATAACAATTTTAGGATATATATAATATTGATACGATTGTAATATTTGTGAATGCAGCTGCAACAACAACGTTCGTGCGGTTCTTCCCTCGAATAAAGGCGAGGTATGACTATGGCTTCCTCATCTTCATCTTGACCTTCTGTTTGGTATCAGTCTCTGGCTATCGAGATGACGAGATTCTTCAAGTCGCCTACACTAGAGCCCTCACCATTCTCATCGGCACCTTCATCGCGATTCTTATTTGCATCTTCATTTGCCCTGTCTGGGCCGGCGAAGATCTCCACTCCCTCGTCTCCAACAATGTTGAACTCCTCGGCAACTTCCTCCAAGGTCCACTCTTAGTTTTGAGTTCTCTTATCAATTATATGATAATGAGACAAATGTTAAATTATAAGTTAATGAGACAAATGTTAAATTATAAGTTTGATCTACTGTTAGTGCTTATTTAGTTACATACTTTAAAAAGTTTAGTTAAGCTATAAGTTCTAGTCCATGAACCTCTCAACAACTCTAAACTTTCAAATGTTGTGTCTAATAGTTCTCAAAACTTCATGAGTTTGTCCATTGGTACAAAGGGACCAAAGAAATTCAAAAGGAATTTAGAGGAAATAGGTTCAAATCTCATATCGTGGCCACCTATCTAAGATTTAATATCCTATATGTGTTCTGTCCTGTGAGATTAGTTCCATGTTCGTATAAACTGGTCGGGCACTCACGGATATAAAAAAAATGAATTAATTAGGAAAATCTAGACACGAGATTAAAAGTTTAGGAACCTGTTATTTGAAACGTATACATACTAAATAGACATAACTTTGAAAATTCAAACTTTTTTTTTCACAACCTATTAAAGTACATGAACGATTAAGAATAGACATGAGCTACTTCTCTTATTGCCAATTAGTTTTGATATAGAACCCTATAAATATTGATATAGTATCAGAGTTCAAAACGTTCAAACGAGCATCTAATCCAAAAAATTGGATCCGGCCAAAATCAATGAGCCAAAAGAACCACAATCTCGGGAAGACATGCTAAGAATAGACATGGGCTATTCCTTTCATTGACAATTGGTTTTGATATGGAACTCCATGAATATTGATATAAATAAATATTGTCGTTCGAGATATCAACGACTTATAATTTAAGCCTAAAACAAACTGAGTTTTGAGTATTAATTTGCACATGTAGGTTTTGGGGCTCGATATTCGGATGAATGGAAAGGAGATAAGCAGGTTGAAGGGTGTAAGAGTGTTTTGACATCGAGGCAAACTGAAGAGTCACTGGTTAACTTTGCAAGGTGGGAACCAGGCCATGGGAGCTTCAAGTTTCGCCATCCATGGAAGCAATATCGCAAGATCGGAAGCTTGACGAGGCAATGTGCTTATCGACTCGAGTCTCTTAATGGCTACCTCACCACTGAAACCCAAGTAAGAAACTTTTCTTCAAACTAGATTAAACAGCTCCCCATTTGATCCTTCGGAGAGACTTGTTTCAATTTATTACTATCATCTTTTGAAAATTTATTTTGATAAAATGACATGTTTTCTTTAACCTTTTTAATCTACAATATGACAAAAATGAACTAGTGTGTTATCTGGCTAACTATGTTAGTTTTCAATATGACAAAAATGAACTAGTGTGTTATCTGGCTAACTATGTTAGTTTTCAATATGACAAAAATGAACTAGTGTGTTATCTGGCTAACTATGTTAGTTTTTACCCTAACAGGAATGAACTAAAACTAATCTTTGACCATAACTAATGAACTTCTATTCCCTTTGGTGCAGATCCCATTACATATCCGAGACCAACTCAAGGACTCGTGCTCAAAAATGAGCATTGAATCAGGCAAAGCTCTGAAGGATCTAGCATCTGCGATAAGAACGATGACTAGACCGACGTTTCCAAATCCCCACGTCGAGAAGTCAAAGGCAGCAGCGAAGAACCTCAAGGTGGCACTGAAGATCGGGCCATGCAACGATGGGATCGATCTTCTCGAGATAGTTCCCGCGGCGACTGTGGCTTCCCTCCTCATTGATTCCATTTCTTGCATTGAGAAGATTGCAGAGTCTGTTGGAGAATTGGCCTCAATGGCACAGTTCAAGAAATGTGTCGAAGCTGGGAAATTAGCTGGCTTGGAATTGGAACAAGATCAGCAACAAAATTTGCCAACTCCTGCCATAGTTAATGGTCATTGTCATGTTGTACTGTAGATTAGGGATAATTAATGTGCCTCTTTTATATACTAATCTTTTTTCTTTCAATTGTGTAATTGAAATGTATCATGATTATGATCCATATTGTATAATAAGAGATTTAGGAATTATTTACACCAAACATGAGCTACAGTAAAACTTCTACTTCTTTTTCTGGAGATTGGAGGTTAAATTTTCG

mRNA sequence

CAAAGAGTAGACGCCATTAGCAAAACCTCATCATACCCCTCTCTCTCTCTCAAATTTTCCAAAAAAGATTTCAGCTACTTCAGCTTATGGAGATGGCTGATCATAAGAGTGAGGGAGTTTCGTCAGCTCGGTGGGCCGAGGGGCTCAGAGCCAAATCCTCGAGACTAACGGTGAAGGTGGGTGAGTTAGCGAGGAAGACAAAGAAGCTGGCCAAGGATGATCCGAGAAGAATCGCTCATTCCCTCAAAGTAGCCTTAGCCATCACTTTGGTTTCATTGTTCTACTACTTCGAACCGCTGTATGATGGCTTTGGTGCCTCCGCTATGTGGGCTATCTTAACAGTTGTAGTCGTCTTCGAGTTCTCTGTAGGAGCAACACTTGGACGAGGATTGAACAGAGTTTTGGCAACATTTTCGGCTGCGGCTCTGGGATTTGGAGCCCATTTTTTGGCAGATTTGGCTGCAGACAAAGGCGAACCCATTATTCTTGCTTTCTTTGTTTTCTTACTTGCTGCAACAACAACGTTCGTGCGGTTCTTCCCTCGAATAAAGGCGAGGTATGACTATGGCTTCCTCATCTTCATCTTGACCTTCTGTTTGGTATCAGTCTCTGGCTATCGAGATGACGAGATTCTTCAAGTCGCCTACACTAGAGCCCTCACCATTCTCATCGGCACCTTCATCGCGATTCTTATTTGCATCTTCATTTGCCCTGTCTGGGCCGGCGAAGATCTCCACTCCCTCGTCTCCAACAATGTTGAACTCCTCGGCAACTTCCTCCAAGGTTTTGGGGCTCGATATTCGGATGAATGGAAAGGAGATAAGCAGGTTGAAGGGTGTAAGAGTGTTTTGACATCGAGGCAAACTGAAGAGTCACTGGTTAACTTTGCAAGGTGGGAACCAGGCCATGGGAGCTTCAAGTTTCGCCATCCATGGAAGCAATATCGCAAGATCGGAAGCTTGACGAGGCAATGTGCTTATCGACTCGAGTCTCTTAATGGCTACCTCACCACTGAAACCCAAATCCCATTACATATCCGAGACCAACTCAAGGACTCGTGCTCAAAAATGAGCATTGAATCAGGCAAAGCTCTGAAGGATCTAGCATCTGCGATAAGAACGATGACTAGACCGACGTTTCCAAATCCCCACGTCGAGAAGTCAAAGGCAGCAGCGAAGAACCTCAAGGTGGCACTGAAGATCGGGCCATGCAACGATGGGATCGATCTTCTCGAGATAGTTCCCGCGGCGACTGTGGCTTCCCTCCTCATTGATTCCATTTCTTGCATTGAGAAGATTGCAGAGTCTGTTGGAGAATTGGCCTCAATGGCACAGTTCAAGAAATGTGTCGAAGCTGGGAAATTAGCTGGCTTGGAATTGGAACAAGATCAGCAACAAAATTTGCCAACTCCTGCCATAGTTAATGGTCATTGTCATGTTGTACTGTAGATTAGGGATAATTAATGTGCCTCTTTTATATACTAATCTTTTTTCTTTCAATTGTGTAATTGAAATGTATCATGATTATGATCCATATTGTATAATAAGAGATTTAGGAATTATTTACACCAAACATGAGCTACAGTAAAACTTCTACTTCTTTTTCTGGAGATTGGAGGTTAAATTTTCG

Coding sequence (CDS)

ATGGAGATGGCTGATCATAAGAGTGAGGGAGTTTCGTCAGCTCGGTGGGCCGAGGGGCTCAGAGCCAAATCCTCGAGACTAACGGTGAAGGTGGGTGAGTTAGCGAGGAAGACAAAGAAGCTGGCCAAGGATGATCCGAGAAGAATCGCTCATTCCCTCAAAGTAGCCTTAGCCATCACTTTGGTTTCATTGTTCTACTACTTCGAACCGCTGTATGATGGCTTTGGTGCCTCCGCTATGTGGGCTATCTTAACAGTTGTAGTCGTCTTCGAGTTCTCTGTAGGAGCAACACTTGGACGAGGATTGAACAGAGTTTTGGCAACATTTTCGGCTGCGGCTCTGGGATTTGGAGCCCATTTTTTGGCAGATTTGGCTGCAGACAAAGGCGAACCCATTATTCTTGCTTTCTTTGTTTTCTTACTTGCTGCAACAACAACGTTCGTGCGGTTCTTCCCTCGAATAAAGGCGAGGTATGACTATGGCTTCCTCATCTTCATCTTGACCTTCTGTTTGGTATCAGTCTCTGGCTATCGAGATGACGAGATTCTTCAAGTCGCCTACACTAGAGCCCTCACCATTCTCATCGGCACCTTCATCGCGATTCTTATTTGCATCTTCATTTGCCCTGTCTGGGCCGGCGAAGATCTCCACTCCCTCGTCTCCAACAATGTTGAACTCCTCGGCAACTTCCTCCAAGGTTTTGGGGCTCGATATTCGGATGAATGGAAAGGAGATAAGCAGGTTGAAGGGTGTAAGAGTGTTTTGACATCGAGGCAAACTGAAGAGTCACTGGTTAACTTTGCAAGGTGGGAACCAGGCCATGGGAGCTTCAAGTTTCGCCATCCATGGAAGCAATATCGCAAGATCGGAAGCTTGACGAGGCAATGTGCTTATCGACTCGAGTCTCTTAATGGCTACCTCACCACTGAAACCCAAATCCCATTACATATCCGAGACCAACTCAAGGACTCGTGCTCAAAAATGAGCATTGAATCAGGCAAAGCTCTGAAGGATCTAGCATCTGCGATAAGAACGATGACTAGACCGACGTTTCCAAATCCCCACGTCGAGAAGTCAAAGGCAGCAGCGAAGAACCTCAAGGTGGCACTGAAGATCGGGCCATGCAACGATGGGATCGATCTTCTCGAGATAGTTCCCGCGGCGACTGTGGCTTCCCTCCTCATTGATTCCATTTCTTGCATTGAGAAGATTGCAGAGTCTGTTGGAGAATTGGCCTCAATGGCACAGTTCAAGAAATGTGTCGAAGCTGGGAAATTAGCTGGCTTGGAATTGGAACAAGATCAGCAACAAAATTTGCCAACTCCTGCCATAGTTAATGGTCATTGTCATGTTGTACTGTAG

Protein sequence

MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSDEWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKCVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVVL
Homology
BLAST of MC06g1062 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 414.1 bits (1063), Expect = 2.1e-114
Identity = 210/398 (52.76%), Postives = 292/398 (73.37%), Query Frame = 0

Query: 30  KVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAILTVVVV 89
           KV E+ R+ +++ K+DPRR+ H+ KV LA+ LVS FYY++PLYD FG +AMWA++TVVVV
Sbjct: 3   KVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVV 62

Query: 90  FEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTTFVR 149
           FEFSVGATLG+GLNR +AT  A  LG GAH LA L+    EPI+LA FVF+LAA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVR 122

Query: 150 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIFICP 209
           FFPR+KARYDYG LIFILTF L+SVSG+R+DEIL +A+ R  T+++G    +LI IF+CP
Sbjct: 123 FFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCP 182

Query: 210 VWAGEDLHSLVSNNVELLGNFLQGFGARYSDEWK-GD--------KQVEGCKSVLTSRQT 269
           VWAG+DLHSL+++N + L +FLQ FG  Y +  + GD        + +E  KSVL S+  
Sbjct: 183 VWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSN 242

Query: 270 EESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYLTTETQIPLHIRDQ 329
           EE+L NFA+WEP HG F+FRHPW+QY  +G+L RQ AYR+++LN  + ++ QIP+ I+ +
Sbjct: 243 EEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKK 302

Query: 330 LKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAAAKNLKVALKIGPCNDGID 389
           +++   +MS ESGK++K+++ +++ MT  +  + HV  S++A K L   LK G  ND ++
Sbjct: 303 IEEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND-VE 362

Query: 390 LLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFK 419
            L+++   T  SLLID ++  EKI+ESV ELAS A+FK
Sbjct: 363 PLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK 399

BLAST of MC06g1062 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 3.5e-114
Identity = 223/441 (50.57%), Postives = 286/441 (64.85%), Query Frame = 0

Query: 25  SRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAIL 84
           S+L   V +  +  +K AKDDPRRI HS+KV +A+TLVSL YY  PLY  FG + MWAIL
Sbjct: 21  SKLKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAIL 80

Query: 85  TVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAAT 144
           TVVVVFEF+VG TL +GLNR  AT  A ALG GA  LA     +GEPI+L   VF L A 
Sbjct: 81  TVVVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAA 140

Query: 145 TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILIC 204
            TF RFFPRIK RYDYG LIFILTF  V++SGYR DEIL +AY R  TILIG  I IL+ 
Sbjct: 141 ATFSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVS 200

Query: 205 IFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSDEWKGDKQVEGC----KSVLTSRQT 264
           IFICPVWAGEDLH +++NN+  L  +L+GF   Y    K  K+   C    KS+LTS+ T
Sbjct: 201 IFICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKST 260

Query: 265 EESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYLTTETQIPLHIRDQ 324
           E+SL N ARWEPGHG F+ RHPWK+Y KI  L RQCA  LE LNGY+ +  + P     +
Sbjct: 261 EDSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSNDKAPQEFESK 320

Query: 325 LKDSCSKMSIESGKALKDLASAIRTMTRPT-FPNPHVEKSKAAAKNLKVALKIGPCNDGI 384
           +++  + MS E G+ALK +A +I+TM   +   N H++ SK A KNLK+ALK        
Sbjct: 321 IQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYK 380

Query: 385 DLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKCVEAGKLAGLELEQDQQQNL 444
           DLLEI+P  T+AS+LI+ ++C+EKI E+V E + +A FK+ +++   A +   Q   +  
Sbjct: 381 DLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKETLDSKLSAEIGQHQLLHRGC 440

Query: 445 PTPAI-------VNGHCHVVL 454
             P +        N  CHV++
Sbjct: 441 VKPVLDGDNEKEDNSSCHVLI 461

BLAST of MC06g1062 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.0e-113
Identity = 211/428 (49.30%), Postives = 291/428 (67.99%), Query Frame = 0

Query: 30  KVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAILTVVVV 89
           KV E+ R+ +++AK+DPRR+ HS KV L + LVS FYY++PLYD FG +AMWA++TVVVV
Sbjct: 3   KVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVV 62

Query: 90  FEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTTFVR 149
           FEFSVGATLG+GLNRV AT  A  LG GAH LA ++   GEPI+LA FVF+ AA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVR 122

Query: 150 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIFICP 209
           FFPR+KARYDY  LIFILTF L+SVSG+R+++++++ + R  T++IG    ++I IF+CP
Sbjct: 123 FFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCP 182

Query: 210 VWAGEDLHSLVSNNVELLGNFLQG--------------------------FGARYSDEWK 269
           VWAG+DLHSL+++N E L  FL G                          FG +Y +  +
Sbjct: 183 VWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVE 242

Query: 270 GD---------KQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTR 329
            D         K  +  KSVL S+  EESL NFA+WEPGHG F+FRHPWKQY  +G L R
Sbjct: 243 NDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIR 302

Query: 330 QCAYRLESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNP 389
           QCAYR+ +LN YL  + Q+ + I+ +L +   +MS+ESGKA+K+++ +++ MT+P+  + 
Sbjct: 303 QCAYRIHALNSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDL 362

Query: 390 HVEKSKAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASM 423
           HV+ +K+A K+L   L  G   + ++ LE+V   T  SLLID I+  EKI ES+ ELA+ 
Sbjct: 363 HVQNAKSACKSLTNLLNSGILKE-VEPLELVSLLTAISLLIDIINLTEKILESLHELATA 422

BLAST of MC06g1062 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.2e-106
Identity = 204/401 (50.87%), Postives = 283/401 (70.57%), Query Frame = 0

Query: 30  KVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFE---PLYDGFGASAMWAILTV 89
           KV E+ R+  ++  +DPRRI H+ KV LA+ LVS FYY++   P  D FG +AMWA++TV
Sbjct: 3   KVREIVREGIRVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTV 62

Query: 90  VVVFEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTT 149
           VVVFEFSVGATLG+GLNR +AT  A  LG GAH LA L+    EPI+L   VF+ AA +T
Sbjct: 63  VVVFEFSVGATLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALST 122

Query: 150 FVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIF 209
           FVRFFP +K ++DYG LIFILTF L+S+SG+RD+EI+ +A +R  T++IG    ILI IF
Sbjct: 123 FVRFFPWVKTKFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIF 182

Query: 210 ICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD-EWKGD--------KQVEGCKSVLTS 269
           +CPVWAG+DLHSL+++N + L +FLQ FG  Y +   KGD        K +E  KSVL S
Sbjct: 183 VCPVWAGQDLHSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDS 242

Query: 270 RQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYLTTETQIPLHI 329
           +  EE+L N+A WEP HG F+FRHPWKQY  +G+L RQCAYR+++LN Y+ ++ QIP+ I
Sbjct: 243 KSDEEALANYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDI 302

Query: 330 RDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAAAKNLKVALKIGPCND 389
           + +L+    +MS ESG ++K+++ +++ M + +  + HV  S+AA K+L   LK G  ND
Sbjct: 303 KKKLETPLRRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILND 362

Query: 390 GIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFK 419
            ++ L+++   T  S+LID ++  EKI+ESV ELAS A+FK
Sbjct: 363 -VEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK 402

BLAST of MC06g1062 vs. ExPASy Swiss-Prot
Match: Q76LB1 (Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.6e-93
Identity = 193/426 (45.31%), Postives = 273/426 (64.08%), Query Frame = 0

Query: 35  ARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSV 94
           ARK    A++DPRR+AHSLKV LA+ LVS+ Y+  PL++G G SA+WA+LTVVVV E++V
Sbjct: 39  ARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGVSAIWAVLTVVVVMEYTV 98

Query: 95  GATLGRGLNRVLATFSAAALGFGAHFLADLA---ADKGEPIILAFFVFLLAATTTFVRFF 154
           GATL +GLNR LAT  A  +  GAH LA+LA    D+GEPI+L   VF +A+  TF+RF 
Sbjct: 99  GATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVLTVLVFFVASAATFLRFI 158

Query: 155 PRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIFICPVW 214
           P IKA+YDYG  IFILTF LV+VS YR +E++Q+A+ R  TI +G FI +   +F+ PVW
Sbjct: 159 PEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIAVGVFICLCTTVFLFPVW 218

Query: 215 AGEDLHSLVSNNVELLGNFLQG-----FGAR-YSDEWKGDKQVEGCKSVLTSRQTEESLV 274
           AGED+H L S N++ L  F++G     FG    ++ + G    +  KSVL S+ TE+SL 
Sbjct: 219 AGEDVHKLASGNLDKLAQFIEGMEFNCFGENSVANNFGGKDSPQMHKSVLNSKATEDSLC 278

Query: 275 NFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYL--TTETQIPLHIRDQL-- 334
            FA+WEP HG F+FRHPW QY+K+G+L RQCA  +E+L  Y+  T++TQ P     +L  
Sbjct: 279 TFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALASYVITTSKTQCPAAANPELSC 338

Query: 335 --KDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAAAKNLKVALKIGPCNDGI 394
             + +C +MS+ S K L+DLA A RTMT P+  N  +  +  AA++L+  L      +  
Sbjct: 339 KVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMATAVKAAESLRSELA-----ENT 398

Query: 395 DLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKCVEAGKLAGLELEQDQQQNL 446
            LL+++  A  A+LL D +  +++IAE V  LA +A FK   +   +    + +   + L
Sbjct: 399 ALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAHFKNPEDTKNVVVSTVSRGIDEPL 458

BLAST of MC06g1062 vs. NCBI nr
Match: XP_022141905.1 (aluminum-activated malate transporter 2-like [Momordica charantia])

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT
Sbjct: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF
Sbjct: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD
Sbjct: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD
Sbjct: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240

Query: 241 EWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRL 300
           EWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRL
Sbjct: 241 EWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRL 300

Query: 301 ESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSK 360
           ESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSK
Sbjct: 301 ESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSK 360

Query: 361 AAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKC 420
           AAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKC
Sbjct: 361 AAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKC 420

Query: 421 VEAGKLAGLELEQDQQQNLPTPAIVNGHCHVVL 453
           VEAGKLAGLELEQDQQQNLPTPAIVNGHCHVVL
Sbjct: 421 VEAGKLAGLELEQDQQQNLPTPAIVNGHCHVVL 453

BLAST of MC06g1062 vs. NCBI nr
Match: XP_038880035.1 (aluminum-activated malate transporter 8-like [Benincasa hispida])

HSP 1 Score: 733 bits (1892), Expect = 8.00e-264
Identity = 379/452 (83.85%), Postives = 405/452 (89.60%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEMA+ KSEGV  ARW EGL AK S+L  KV EL RKTKKLAKDDPRRI HSLKV LA+T
Sbjct: 1   MEMANDKSEGVL-ARWGEGLEAKFSKLKAKVIELFRKTKKLAKDDPRRIVHSLKVGLALT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWAILTVVVVFEFSVGATLGRGLNRVLATF AAALGFGAHF
Sbjct: 61  LVSLFYYFEPLYDGLGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFFAAALGFGAHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA DK +PI+L+  VF LA  TTFVRFFPR+KARYDYGFLIFILTFCLVSVSGYR+D
Sbjct: 121 LADLAGDKAQPIMLSLSVFFLATITTFVRFFPRLKARYDYGFLIFILTFCLVSVSGYRED 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EIL VAY RALTILIGTFIAILICI ICPVWAG+DLHSLVSNN+ELL NFL+GFG +YS+
Sbjct: 181 EILNVAYRRALTILIGTFIAILICICICPVWAGDDLHSLVSNNIELLANFLEGFGGQYSN 240

Query: 241 EWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRL 300
           EWK ++ VE CKSVLTSRQTEESLVNFARWEPGHG+FKFRHPWKQYRKIGSLTRQCAYRL
Sbjct: 241 EWKENEVVEECKSVLTSRQTEESLVNFARWEPGHGTFKFRHPWKQYRKIGSLTRQCAYRL 300

Query: 301 ESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSK 360
           ESLN YL TE Q PLHIRDQLK+SCSKMSIES KALK LASAIRTMTRPTFPNPH+E+SK
Sbjct: 301 ESLNTYLVTECQTPLHIRDQLKESCSKMSIESSKALKVLASAIRTMTRPTFPNPHIERSK 360

Query: 361 AAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKC 420
           A AK+LK ALKI PC+ G+DLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A FK C
Sbjct: 361 AVAKDLKAALKIRPCDSGMDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFK-C 420

Query: 421 VEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452
           VE GK A ++LEQ Q+Q LPTPAIVNGHCHVV
Sbjct: 421 VEVGKSASMKLEQHQEQKLPTPAIVNGHCHVV 450

BLAST of MC06g1062 vs. NCBI nr
Match: XP_004139139.1 (aluminum-activated malate transporter 2 [Cucumis sativus] >KGN66582.1 hypothetical protein Csa_007283 [Cucumis sativus])

HSP 1 Score: 706 bits (1821), Expect = 5.35e-253
Identity = 368/453 (81.24%), Postives = 398/453 (87.86%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEMA+ KS G+  A W+EGL+AK S++  KV EL +KTKKLAKDDPRR+ HSLKV LAIT
Sbjct: 1   MEMANEKSGGLL-ASWSEGLKAKISKIMAKVIELFKKTKKLAKDDPRRVVHSLKVGLAIT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWAILTVVVVFEFS+GATLGRGLNRVLATF AAALGFGAHF
Sbjct: 61  LVSLFYYFEPLYDGLGASAMWAILTVVVVFEFSIGATLGRGLNRVLATFLAAALGFGAHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA D  +PI+L+  VF LAA TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYR+D
Sbjct: 121 LADLAGDTAQPIMLSLSVFFLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRED 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EIL+VAY RALTILIGTFIAILICI ICPVWAG+DLHSLVSNN+E L NF QGFG  YS+
Sbjct: 181 EILKVAYRRALTILIGTFIAILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSN 240

Query: 241 EWKGDKQ-VEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYR 300
           EWK D+  VEG KSVLTSRQTEESLVNFARWEPGHG+FKFRHPWKQYRKIGSLTRQCAYR
Sbjct: 241 EWKEDEGIVEGFKSVLTSRQTEESLVNFARWEPGHGTFKFRHPWKQYRKIGSLTRQCAYR 300

Query: 301 LESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKS 360
           LESLN YL  E+Q PLHIRDQLK+SCSKMS ESGKALKDLAS+IRTMT P  PNPH+EKS
Sbjct: 301 LESLNTYLLAESQTPLHIRDQLKESCSKMSTESGKALKDLASSIRTMTLPRLPNPHIEKS 360

Query: 361 KAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKK 420
           KAAAK+LK ALKI PCN  IDLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A FK+
Sbjct: 361 KAAAKDLKAALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFKR 420

Query: 421 CVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452
             E  K A L+ +Q+QQQ L TPAIV+GHCHVV
Sbjct: 421 -FEVEKSASLKFQQEQQQKLATPAIVSGHCHVV 451

BLAST of MC06g1062 vs. NCBI nr
Match: XP_008450296.1 (PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo] >TYK28112.1 aluminum-activated malate transporter 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 705 bits (1819), Expect = 1.08e-252
Identity = 366/453 (80.79%), Postives = 399/453 (88.08%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEM++ KS G + ARW++GL+AK S+L  KV EL +KTKKLAKDDPRRI HSLKV LAIT
Sbjct: 1   MEMSNEKSGG-ALARWSDGLKAKISKLKAKVIELFKKTKKLAKDDPRRIVHSLKVGLAIT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWAILTVVVVFEFSVGAT GRGLNRVLATF AAALGFG HF
Sbjct: 61  LVSLFYYFEPLYDGLGASAMWAILTVVVVFEFSVGATFGRGLNRVLATFLAAALGFGVHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA DK +PI+L+  VF LAA TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYR+D
Sbjct: 121 LADLAGDKAQPIMLSLSVFFLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRED 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EIL+VAY RALTILIGTFIAILICI ICPVWAG+DLHSLVSNN+E L NF QGFG  YS+
Sbjct: 181 EILKVAYRRALTILIGTFIAILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSN 240

Query: 241 EWKGDKQ-VEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYR 300
           EWK D++ VEG KSVL+SRQTEESLVNFARWEP HG+FKFRHPWKQY +IGSLTRQCAYR
Sbjct: 241 EWKEDERIVEGFKSVLSSRQTEESLVNFARWEPPHGTFKFRHPWKQYSRIGSLTRQCAYR 300

Query: 301 LESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKS 360
           LESLN YL  E+Q PLHIRDQLK+SC+KMS ESGKALKDLAS+IRTMT P  PNPH+EKS
Sbjct: 301 LESLNTYLLAESQTPLHIRDQLKESCTKMSTESGKALKDLASSIRTMTLPRLPNPHIEKS 360

Query: 361 KAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKK 420
           KAAAK+LK ALKI PCN  IDLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A FK 
Sbjct: 361 KAAAKDLKAALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFK- 420

Query: 421 CVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452
           C+E GK A L+ +Q+QQQ L TPAIV+GHCHVV
Sbjct: 421 CLEVGKSASLKFQQEQQQKLATPAIVSGHCHVV 451

BLAST of MC06g1062 vs. NCBI nr
Match: XP_022988110.1 (aluminum-activated malate transporter 2-like [Cucurbita maxima])

HSP 1 Score: 704 bits (1818), Expect = 1.32e-252
Identity = 365/451 (80.93%), Postives = 399/451 (88.47%), Query Frame = 0

Query: 3   MADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAITLV 62
           M+D K+EGV  ARW EGL+AKSS L  KV EL RKTKKLAKDDPRRI HS KV LAITLV
Sbjct: 1   MSDEKNEGVL-ARWGEGLKAKSSSLKAKVVELLRKTKKLAKDDPRRIVHSFKVGLAITLV 60

Query: 63  SLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHFLA 122
           SLFYYFEPLYDG GASAMWA+LTVVVVFEFSVGATLGRGLNRVLAT  AA+LGFGAHFLA
Sbjct: 61  SLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATSLAASLGFGAHFLA 120

Query: 123 DLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEI 182
           DLA DK +P+IL+  VF+ AA TTF+RFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEI
Sbjct: 121 DLAGDKAQPVILSLSVFIFAAITTFIRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEI 180

Query: 183 LQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSDEW 242
           LQVAY RALTIL+GTFIAILIC+FICPVWAG+DLHSLVSNN++LL NFLQGFG +YS+E 
Sbjct: 181 LQVAYRRALTILLGTFIAILICLFICPVWAGDDLHSLVSNNIQLLANFLQGFGVQYSNES 240

Query: 243 KGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLES 302
           K D+ +EGCKSVLTSRQTEESLVNFARWEP HG+FKFRHPWKQYRKIGSLTRQ AYRLES
Sbjct: 241 KEDELLEGCKSVLTSRQTEESLVNFARWEPRHGAFKFRHPWKQYRKIGSLTRQSAYRLES 300

Query: 303 LNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAA 362
           LN YL TETQ PLHIRD+L  SCSKMSIES  AL+D+AS +RTMT+PTFPNPH+EKSKAA
Sbjct: 301 LNSYLVTETQTPLHIRDRLMKSCSKMSIESSNALEDIASDLRTMTQPTFPNPHIEKSKAA 360

Query: 363 AKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKCVE 422
            K+LK ALKIGP +  IDLLEIVP ATVASLLID+ISCIEKIAESVGEL S+A FK CVE
Sbjct: 361 MKDLKAALKIGPGDGDIDLLEIVPIATVASLLIDTISCIEKIAESVGELGSLANFK-CVE 420

Query: 423 AGKLAGLELEQDQQQNLPTPAIVNGHCHVVL 453
           AGK +GLELEQ+ Q+ LP  AIVN HCHVV+
Sbjct: 421 AGKSSGLELEQEHQK-LPNSAIVNDHCHVVI 448

BLAST of MC06g1062 vs. ExPASy TrEMBL
Match: A0A6J1CJE7 (aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=LOC111012160 PE=3 SV=1)

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT
Sbjct: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF
Sbjct: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD
Sbjct: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD
Sbjct: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240

Query: 241 EWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRL 300
           EWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRL
Sbjct: 241 EWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRL 300

Query: 301 ESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSK 360
           ESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSK
Sbjct: 301 ESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSK 360

Query: 361 AAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKC 420
           AAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKC
Sbjct: 361 AAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKC 420

Query: 421 VEAGKLAGLELEQDQQQNLPTPAIVNGHCHVVL 453
           VEAGKLAGLELEQDQQQNLPTPAIVNGHCHVVL
Sbjct: 421 VEAGKLAGLELEQDQQQNLPTPAIVNGHCHVVL 453

BLAST of MC06g1062 vs. ExPASy TrEMBL
Match: A0A0A0M0C7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630860 PE=3 SV=1)

HSP 1 Score: 706 bits (1821), Expect = 2.59e-253
Identity = 368/453 (81.24%), Postives = 398/453 (87.86%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEMA+ KS G+  A W+EGL+AK S++  KV EL +KTKKLAKDDPRR+ HSLKV LAIT
Sbjct: 1   MEMANEKSGGLL-ASWSEGLKAKISKIMAKVIELFKKTKKLAKDDPRRVVHSLKVGLAIT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWAILTVVVVFEFS+GATLGRGLNRVLATF AAALGFGAHF
Sbjct: 61  LVSLFYYFEPLYDGLGASAMWAILTVVVVFEFSIGATLGRGLNRVLATFLAAALGFGAHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA D  +PI+L+  VF LAA TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYR+D
Sbjct: 121 LADLAGDTAQPIMLSLSVFFLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRED 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EIL+VAY RALTILIGTFIAILICI ICPVWAG+DLHSLVSNN+E L NF QGFG  YS+
Sbjct: 181 EILKVAYRRALTILIGTFIAILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSN 240

Query: 241 EWKGDKQ-VEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYR 300
           EWK D+  VEG KSVLTSRQTEESLVNFARWEPGHG+FKFRHPWKQYRKIGSLTRQCAYR
Sbjct: 241 EWKEDEGIVEGFKSVLTSRQTEESLVNFARWEPGHGTFKFRHPWKQYRKIGSLTRQCAYR 300

Query: 301 LESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKS 360
           LESLN YL  E+Q PLHIRDQLK+SCSKMS ESGKALKDLAS+IRTMT P  PNPH+EKS
Sbjct: 301 LESLNTYLLAESQTPLHIRDQLKESCSKMSTESGKALKDLASSIRTMTLPRLPNPHIEKS 360

Query: 361 KAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKK 420
           KAAAK+LK ALKI PCN  IDLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A FK+
Sbjct: 361 KAAAKDLKAALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFKR 420

Query: 421 CVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452
             E  K A L+ +Q+QQQ L TPAIV+GHCHVV
Sbjct: 421 -FEVEKSASLKFQQEQQQKLATPAIVSGHCHVV 451

BLAST of MC06g1062 vs. ExPASy TrEMBL
Match: A0A5D3DX63 (Aluminum-activated malate transporter 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00170 PE=3 SV=1)

HSP 1 Score: 705 bits (1819), Expect = 5.22e-253
Identity = 366/453 (80.79%), Postives = 399/453 (88.08%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEM++ KS G + ARW++GL+AK S+L  KV EL +KTKKLAKDDPRRI HSLKV LAIT
Sbjct: 1   MEMSNEKSGG-ALARWSDGLKAKISKLKAKVIELFKKTKKLAKDDPRRIVHSLKVGLAIT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWAILTVVVVFEFSVGAT GRGLNRVLATF AAALGFG HF
Sbjct: 61  LVSLFYYFEPLYDGLGASAMWAILTVVVVFEFSVGATFGRGLNRVLATFLAAALGFGVHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA DK +PI+L+  VF LAA TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYR+D
Sbjct: 121 LADLAGDKAQPIMLSLSVFFLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRED 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EIL+VAY RALTILIGTFIAILICI ICPVWAG+DLHSLVSNN+E L NF QGFG  YS+
Sbjct: 181 EILKVAYRRALTILIGTFIAILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSN 240

Query: 241 EWKGDKQ-VEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYR 300
           EWK D++ VEG KSVL+SRQTEESLVNFARWEP HG+FKFRHPWKQY +IGSLTRQCAYR
Sbjct: 241 EWKEDERIVEGFKSVLSSRQTEESLVNFARWEPPHGTFKFRHPWKQYSRIGSLTRQCAYR 300

Query: 301 LESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKS 360
           LESLN YL  E+Q PLHIRDQLK+SC+KMS ESGKALKDLAS+IRTMT P  PNPH+EKS
Sbjct: 301 LESLNTYLLAESQTPLHIRDQLKESCTKMSTESGKALKDLASSIRTMTLPRLPNPHIEKS 360

Query: 361 KAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKK 420
           KAAAK+LK ALKI PCN  IDLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A FK 
Sbjct: 361 KAAAKDLKAALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFK- 420

Query: 421 CVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452
           C+E GK A L+ +Q+QQQ L TPAIV+GHCHVV
Sbjct: 421 CLEVGKSASLKFQQEQQQKLATPAIVSGHCHVV 451

BLAST of MC06g1062 vs. ExPASy TrEMBL
Match: A0A1S3BNY9 (aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC103491946 PE=3 SV=1)

HSP 1 Score: 705 bits (1819), Expect = 5.22e-253
Identity = 366/453 (80.79%), Postives = 399/453 (88.08%), Query Frame = 0

Query: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60
           MEM++ KS G + ARW++GL+AK S+L  KV EL +KTKKLAKDDPRRI HSLKV LAIT
Sbjct: 1   MEMSNEKSGG-ALARWSDGLKAKISKLKAKVIELFKKTKKLAKDDPRRIVHSLKVGLAIT 60

Query: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWAILTVVVVFEFSVGAT GRGLNRVLATF AAALGFG HF
Sbjct: 61  LVSLFYYFEPLYDGLGASAMWAILTVVVVFEFSVGATFGRGLNRVLATFLAAALGFGVHF 120

Query: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA DK +PI+L+  VF LAA TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYR+D
Sbjct: 121 LADLAGDKAQPIMLSLSVFFLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRED 180

Query: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD 240
           EIL+VAY RALTILIGTFIAILICI ICPVWAG+DLHSLVSNN+E L NF QGFG  YS+
Sbjct: 181 EILKVAYRRALTILIGTFIAILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSN 240

Query: 241 EWKGDKQ-VEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYR 300
           EWK D++ VEG KSVL+SRQTEESLVNFARWEP HG+FKFRHPWKQY +IGSLTRQCAYR
Sbjct: 241 EWKEDERIVEGFKSVLSSRQTEESLVNFARWEPPHGTFKFRHPWKQYSRIGSLTRQCAYR 300

Query: 301 LESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKS 360
           LESLN YL  E+Q PLHIRDQLK+SC+KMS ESGKALKDLAS+IRTMT P  PNPH+EKS
Sbjct: 301 LESLNTYLLAESQTPLHIRDQLKESCTKMSTESGKALKDLASSIRTMTLPRLPNPHIEKS 360

Query: 361 KAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKK 420
           KAAAK+LK ALKI PCN  IDLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A FK 
Sbjct: 361 KAAAKDLKAALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFK- 420

Query: 421 CVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452
           C+E GK A L+ +Q+QQQ L TPAIV+GHCHVV
Sbjct: 421 CLEVGKSASLKFQQEQQQKLATPAIVSGHCHVV 451

BLAST of MC06g1062 vs. ExPASy TrEMBL
Match: A0A6J1JIP2 (aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485453 PE=3 SV=1)

HSP 1 Score: 704 bits (1818), Expect = 6.39e-253
Identity = 365/451 (80.93%), Postives = 399/451 (88.47%), Query Frame = 0

Query: 3   MADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAITLV 62
           M+D K+EGV  ARW EGL+AKSS L  KV EL RKTKKLAKDDPRRI HS KV LAITLV
Sbjct: 1   MSDEKNEGVL-ARWGEGLKAKSSSLKAKVVELLRKTKKLAKDDPRRIVHSFKVGLAITLV 60

Query: 63  SLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHFLA 122
           SLFYYFEPLYDG GASAMWA+LTVVVVFEFSVGATLGRGLNRVLAT  AA+LGFGAHFLA
Sbjct: 61  SLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATSLAASLGFGAHFLA 120

Query: 123 DLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEI 182
           DLA DK +P+IL+  VF+ AA TTF+RFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEI
Sbjct: 121 DLAGDKAQPVILSLSVFIFAAITTFIRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEI 180

Query: 183 LQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSDEW 242
           LQVAY RALTIL+GTFIAILIC+FICPVWAG+DLHSLVSNN++LL NFLQGFG +YS+E 
Sbjct: 181 LQVAYRRALTILLGTFIAILICLFICPVWAGDDLHSLVSNNIQLLANFLQGFGVQYSNES 240

Query: 243 KGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLES 302
           K D+ +EGCKSVLTSRQTEESLVNFARWEP HG+FKFRHPWKQYRKIGSLTRQ AYRLES
Sbjct: 241 KEDELLEGCKSVLTSRQTEESLVNFARWEPRHGAFKFRHPWKQYRKIGSLTRQSAYRLES 300

Query: 303 LNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAA 362
           LN YL TETQ PLHIRD+L  SCSKMSIES  AL+D+AS +RTMT+PTFPNPH+EKSKAA
Sbjct: 301 LNSYLVTETQTPLHIRDRLMKSCSKMSIESSNALEDIASDLRTMTQPTFPNPHIEKSKAA 360

Query: 363 AKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKCVE 422
            K+LK ALKIGP +  IDLLEIVP ATVASLLID+ISCIEKIAESVGEL S+A FK CVE
Sbjct: 361 MKDLKAALKIGPGDGDIDLLEIVPIATVASLLIDTISCIEKIAESVGELGSLANFK-CVE 420

Query: 423 AGKLAGLELEQDQQQNLPTPAIVNGHCHVVL 453
           AGK +GLELEQ+ Q+ LP  AIVN HCHVV+
Sbjct: 421 AGKSSGLELEQEHQK-LPNSAIVNDHCHVVI 448

BLAST of MC06g1062 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.5e-115
Identity = 210/398 (52.76%), Postives = 292/398 (73.37%), Query Frame = 0

Query: 30  KVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAILTVVVV 89
           KV E+ R+ +++ K+DPRR+ H+ KV LA+ LVS FYY++PLYD FG +AMWA++TVVVV
Sbjct: 3   KVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVV 62

Query: 90  FEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTTFVR 149
           FEFSVGATLG+GLNR +AT  A  LG GAH LA L+    EPI+LA FVF+LAA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVR 122

Query: 150 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIFICP 209
           FFPR+KARYDYG LIFILTF L+SVSG+R+DEIL +A+ R  T+++G    +LI IF+CP
Sbjct: 123 FFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCP 182

Query: 210 VWAGEDLHSLVSNNVELLGNFLQGFGARYSDEWK-GD--------KQVEGCKSVLTSRQT 269
           VWAG+DLHSL+++N + L +FLQ FG  Y +  + GD        + +E  KSVL S+  
Sbjct: 183 VWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSN 242

Query: 270 EESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYLTTETQIPLHIRDQ 329
           EE+L NFA+WEP HG F+FRHPW+QY  +G+L RQ AYR+++LN  + ++ QIP+ I+ +
Sbjct: 243 EEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKK 302

Query: 330 LKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAAAKNLKVALKIGPCNDGID 389
           +++   +MS ESGK++K+++ +++ MT  +  + HV  S++A K L   LK G  ND ++
Sbjct: 303 IEEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND-VE 362

Query: 390 LLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFK 419
            L+++   T  SLLID ++  EKI+ESV ELAS A+FK
Sbjct: 363 PLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK 399

BLAST of MC06g1062 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 2.5e-115
Identity = 223/441 (50.57%), Postives = 286/441 (64.85%), Query Frame = 0

Query: 25  SRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAIL 84
           S+L   V +  +  +K AKDDPRRI HS+KV +A+TLVSL YY  PLY  FG + MWAIL
Sbjct: 21  SKLKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAIL 80

Query: 85  TVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAAT 144
           TVVVVFEF+VG TL +GLNR  AT  A ALG GA  LA     +GEPI+L   VF L A 
Sbjct: 81  TVVVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAA 140

Query: 145 TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILIC 204
            TF RFFPRIK RYDYG LIFILTF  V++SGYR DEIL +AY R  TILIG  I IL+ 
Sbjct: 141 ATFSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVS 200

Query: 205 IFICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSDEWKGDKQVEGC----KSVLTSRQT 264
           IFICPVWAGEDLH +++NN+  L  +L+GF   Y    K  K+   C    KS+LTS+ T
Sbjct: 201 IFICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKST 260

Query: 265 EESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYLTTETQIPLHIRDQ 324
           E+SL N ARWEPGHG F+ RHPWK+Y KI  L RQCA  LE LNGY+ +  + P     +
Sbjct: 261 EDSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSNDKAPQEFESK 320

Query: 325 LKDSCSKMSIESGKALKDLASAIRTMTRPT-FPNPHVEKSKAAAKNLKVALKIGPCNDGI 384
           +++  + MS E G+ALK +A +I+TM   +   N H++ SK A KNLK+ALK        
Sbjct: 321 IQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYK 380

Query: 385 DLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKKCVEAGKLAGLELEQDQQQNL 444
           DLLEI+P  T+AS+LI+ ++C+EKI E+V E + +A FK+ +++   A +   Q   +  
Sbjct: 381 DLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKETLDSKLSAEIGQHQLLHRGC 440

Query: 445 PTPAI-------VNGHCHVVL 454
             P +        N  CHV++
Sbjct: 441 VKPVLDGDNEKEDNSSCHVLI 461

BLAST of MC06g1062 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 2.1e-114
Identity = 211/428 (49.30%), Postives = 291/428 (67.99%), Query Frame = 0

Query: 30  KVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAILTVVVV 89
           KV E+ R+ +++AK+DPRR+ HS KV L + LVS FYY++PLYD FG +AMWA++TVVVV
Sbjct: 3   KVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVV 62

Query: 90  FEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTTFVR 149
           FEFSVGATLG+GLNRV AT  A  LG GAH LA ++   GEPI+LA FVF+ AA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVR 122

Query: 150 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIFICP 209
           FFPR+KARYDY  LIFILTF L+SVSG+R+++++++ + R  T++IG    ++I IF+CP
Sbjct: 123 FFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCP 182

Query: 210 VWAGEDLHSLVSNNVELLGNFLQG--------------------------FGARYSDEWK 269
           VWAG+DLHSL+++N E L  FL G                          FG +Y +  +
Sbjct: 183 VWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVE 242

Query: 270 GD---------KQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTR 329
            D         K  +  KSVL S+  EESL NFA+WEPGHG F+FRHPWKQY  +G L R
Sbjct: 243 NDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIR 302

Query: 330 QCAYRLESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNP 389
           QCAYR+ +LN YL  + Q+ + I+ +L +   +MS+ESGKA+K+++ +++ MT+P+  + 
Sbjct: 303 QCAYRIHALNSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDL 362

Query: 390 HVEKSKAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASM 423
           HV+ +K+A K+L   L  G   + ++ LE+V   T  SLLID I+  EKI ES+ ELA+ 
Sbjct: 363 HVQNAKSACKSLTNLLNSGILKE-VEPLELVSLLTAISLLIDIINLTEKILESLHELATA 422

BLAST of MC06g1062 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 388.3 bits (996), Expect = 8.6e-108
Identity = 204/401 (50.87%), Postives = 283/401 (70.57%), Query Frame = 0

Query: 30  KVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFE---PLYDGFGASAMWAILTV 89
           KV E+ R+  ++  +DPRRI H+ KV LA+ LVS FYY++   P  D FG +AMWA++TV
Sbjct: 3   KVREIVREGIRVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTV 62

Query: 90  VVVFEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTT 149
           VVVFEFSVGATLG+GLNR +AT  A  LG GAH LA L+    EPI+L   VF+ AA +T
Sbjct: 63  VVVFEFSVGATLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALST 122

Query: 150 FVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIF 209
           FVRFFP +K ++DYG LIFILTF L+S+SG+RD+EI+ +A +R  T++IG    ILI IF
Sbjct: 123 FVRFFPWVKTKFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIF 182

Query: 210 ICPVWAGEDLHSLVSNNVELLGNFLQGFGARYSD-EWKGD--------KQVEGCKSVLTS 269
           +CPVWAG+DLHSL+++N + L +FLQ FG  Y +   KGD        K +E  KSVL S
Sbjct: 183 VCPVWAGQDLHSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDS 242

Query: 270 RQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYLTTETQIPLHI 329
           +  EE+L N+A WEP HG F+FRHPWKQY  +G+L RQCAYR+++LN Y+ ++ QIP+ I
Sbjct: 243 KSDEEALANYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDI 302

Query: 330 RDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAAAKNLKVALKIGPCND 389
           + +L+    +MS ESG ++K+++ +++ M + +  + HV  S+AA K+L   LK G  ND
Sbjct: 303 KKKLETPLRRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILND 362

Query: 390 GIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFK 419
            ++ L+++   T  S+LID ++  EKI+ESV ELAS A+FK
Sbjct: 363 -VEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK 402

BLAST of MC06g1062 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 306.6 bits (784), Expect = 3.3e-83
Identity = 175/433 (40.42%), Postives = 255/433 (58.89%), Query Frame = 0

Query: 30  KVGELARKTKKLAKDDPRRIAHSLKVALAITLVSLFYYFEPLYDGFGASAMWAILTVVVV 89
           +V +  RK  ++  DDP ++ H LKV LA++LVS+FYY  PLYDG G +AMWAI+TVVVV
Sbjct: 48  RVAKFMRKAWRIGADDPAKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNAMWAIMTVVVV 107

Query: 90  FEFSVGATLGRGLNRVLATFSAAALGFGAHFLADLAADKGEPIILAFFVFLLAATTTFVR 149
           FE +VGAT  + +NRV+AT  A +LG   H++A   + K E  ++   VFL A   T+ R
Sbjct: 108 FESNVGATFCKCVNRVVATILAGSLGIAVHWVA-TQSGKAEVFVIGCSVFLFAFAATYSR 167

Query: 150 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAILICIFICP 209
           F P  KAR+DYG +IFILTF LVSV GYR D+++++A  R  TI IGT I I+I +F CP
Sbjct: 168 FVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSICIIITVFFCP 227

Query: 210 VWAGEDLHSLVSNNVELLGNFLQGFGARY----------SDEWKGDKQVEGCKSVLTSRQ 269
           +WAG  LH L+  N+E L + L G  A Y          +++   + +++G K VL S+ 
Sbjct: 228 IWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRNEDENTNMKLQGFKCVLNSKG 287

Query: 270 TEESL------------VNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNGYL 329
           TEE++             N ARWEP HGSF FRHPWK Y KIG+  R+CAY LE+L+  +
Sbjct: 288 TEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCAYCLENLSICI 347

Query: 330 TTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKSKAAAKNLK 389
             ET+ P  +++   ++C K+S  S K L++LA  ++   + +  +  V    +A + L+
Sbjct: 348 NYETEAPDQVKNHFGEACMKLSSASSKILRELADMMKNTRKSSKMDFLVFDMNSAVQELQ 407

Query: 390 VALKIGPC---------------------NDGIDLLEIVPAATVASLLIDSISCIEKIAE 420
             LK  P                         + L E++P AT+ SLLI++ + I+   E
Sbjct: 408 ETLKNVPIETNKPEEVPSEEENKVDSEERTTSMSLHEVLPVATLVSLLIENAARIQTAVE 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJE82.1e-11452.76Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9SRM93.5e-11450.57Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q9XIN13.0e-11349.30Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Q9SJE91.2e-10650.87Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q76LB12.6e-9345.31Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE... [more]
Match NameE-valueIdentityDescription
XP_022141905.10.0100.00aluminum-activated malate transporter 2-like [Momordica charantia][more]
XP_038880035.18.00e-26483.85aluminum-activated malate transporter 8-like [Benincasa hispida][more]
XP_004139139.15.35e-25381.24aluminum-activated malate transporter 2 [Cucumis sativus] >KGN66582.1 hypothetic... [more]
XP_008450296.11.08e-25280.79PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo] >TYK28112... [more]
XP_022988110.11.32e-25280.93aluminum-activated malate transporter 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CJE70.0100.00aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A0A0M0C72.59e-25381.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630860 PE=3 SV=1[more]
A0A5D3DX635.22e-25380.79Aluminum-activated malate transporter 2-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BNY95.22e-25380.79aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1JIP26.39e-25380.93aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G08440.11.5e-11552.76Aluminium activated malate transporter family protein [more]
AT3G11680.12.5e-11550.57Aluminium activated malate transporter family protein [more]
AT2G27240.12.1e-11449.30Aluminium activated malate transporter family protein [more]
AT1G08430.18.6e-10850.87aluminum-activated malate transporter 1 [more]
AT4G00910.13.3e-8340.42Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 40..372
e-value: 2.9E-142
score: 474.8
NoneNo IPR availablePANTHERPTHR31086:SF53ALUMINUM ACTIVATED MALATE TRANSPORTER FAMILY PROTEINcoord: 23..425
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 23..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1062.1MC06g1062.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane