MC06g1061 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1061
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionaluminum-activated malate transporter 2-like
LocationMC06: 10376208 .. 10378711 (-)
RNA-Seq ExpressionMC06g1061
SyntenyMC06g1061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCTCAAAAAACCTCTAATGGGTTTGAAGGGTTACTGCCTTGCTGCTTCTTGTGGGTGAAGGCCATGTCGGGGAAGCTCCCGGCCATGGCGGTCGAGCTAGCAACCGCAACGAAAAGGCTGGCGAGGGACGACCCGAGGAGGGTAGTTCACTCTCTGAAAGTGGGATTAGCCATCACTTTGGTATCTTTGTTTTACTATTTCAGGCCTTTGTATGACGGGTTTGGAGCCTCTACGATGTGGGCCCTCATCTCGGTTATCGTCGTCTTTGAGTTTTCTGTGGGTAGGTGCACCACTAAACTCAAAAGTTTATGTTGGTATTAATATTAATTGGTTAATATTATCTATATTCATAGTAGGTTACATCTCGAAATCAATTGACTAAGAGGAGTAATCCATATATATTATAAATATATGCAAGGTCTCTTTTCTTTTCACCGTGTGATCCTTAACAAATATTACGAGGTTTGAACACAGAGAAGTTTATGTCAATAAGTTACGGTAGATTTAATCTTTTATTCATATTCTTAACTGTGACATGTTTTAAATATCAAACAGGAGGGACGTTGGGGCGTGGTTTGAATAGAGTTATGGCGACACTCGCAGCTGGGGCTCTAGGGTTTGGAGCTCATTACTTGGCGAACTTGGTCGGAGAGAAGGGCCGACCAATAGTGCTTGGTTTCTTTGTGTTCATACCAGGTAAACATATGTAGTTGTCTCTATCCTCTCAAACATTTGCTCGTAATGAAAAATTATTACCACGTCTTGAGGTTTGAAAATGTATATGCAGCTGCAATATCAACATTCATAAGATTCTTCCCAAAGATGAAGGCAAGATATGATTATGGGATGCTCATATTCATTTTGACATTCTGCATGGTCTCAGTTTCGGGATACAGAGATGATGAGATTGTAAAACTTGCACGTGATAGAGTTTTGACAATTCTCATTGGCTGCTCTTTCACTCTCTTTGTATGTATTTTTGTGCGTCCTGTTTGGGCTGGCTCCGATCTCCACCATTTGGTTGCCACCAACATTGATTCCCTTGCCACCTTCTTACAAGGTACAATAATTCTCATATCCTTAAAGAAAACATATAAATGTCTTTGTAATTTTCTTAACTTGATCATTATGTTGGATATGTCAGGCTTTGGTGATCAATATTTTGGATTGCTAGGACAAGGACAGGCCAGCCATGGTGACATCCAAATGTATAGATTGATTCTGAACTCGAAATCGAATGAAGAATCATTGGTGAGTAGATTGACATGTTTGGAGGCTGTTTGATAATATAATCTTTTTTCTTCCTCGTTCATTCTCACCTACTCATAAATCACTATTGAACCAAGAAACTTAAGTGGATGGAATACGACAAAGTTGATTATTTATTTGTATATTTTCACACATAATACTAAATTTTATTTACTTTTTGGGAAATGACTCAGAAATGGTAAAGAGAAACAAAAATATTACTAAACAATCCCTTAATCTCTCTGTCTATATCCCCAAAAATACGTTTTTTTAGTACTATTGCGTGACTGGACAGACTAACCTAGCAAGATGGGAGCCACGCCATTGGAAGTTCAGATATCGCCATCCTTGGAGGCAATACTTGGAAATTGGAAATTTAAATCGGGACTGCGCCTATCGGCTTGAAGTTCTTAATGGTTACCTTAAAACCAACCTAATTCAAGTAAGATTATTGTTGTAGGTTCAATTATATAAGTTTGTCTTTGTACTTTAAAAAGTTTCTAATACATTTTTAAGCTTTTAATTTTATATATGACGTAATTAACTATGCAAAATAGTTAACTTTTAATCACTTATATGGTACAACTTTAACATATTAGATAAAGGAACTTCTTAAATATAACATAGAGACTTAATTAGACTAGCTCTAACCTGAAATTTCAGAGTTTTTACCGCTTTAAAAATTTAGATGGGTTAAGCCTAAAATTTAACCAAGATTATTCTATTAATTCAATTGCTTCTCATCTCATTAATAAACAATGGAACAAAACATATATCTAATGAGACTATATTTTCTTTTGCAGATACCATTATCACAAATCCAAGATCAATTTCAAGACGAATGCTTGAAGATATGCAGCGAATCAGGTAGAAGTTTACGAGAATTGTCATTGGCAATGAAGAAAATGGATCCACCTCTCACTGTCAAACCCCACATGGAGAGAGCAAAAGTTGCAGCTGATAATCTCAAGTCCCATTTGGAAGAATGGAGATTTGAAGACTCAAACGCTATAGAAGTTGTTCGTGTCGCTTCTTTGGTCTCACTTCTCATAGACTCGATATGGTGCGTCGAGAAGATTGGAGACTCAGTTCAAAAATTGGCTTCAGCCGGAGGTTTCAAGGCTGTGGAAGGGCAATCATGTGTAGTAGTGGCACCAGAAGATCAGCTACAAGATTTGGCCAAGGATCATGGAGCCATCCTTTTGGCTCATCATGTCATTACTATTGATGAACAAGAATGTACTCCTTGT

mRNA sequence

ATGGCATCTCAAAAAACCTCTAATGGGTTTGAAGGGTTACTGCCTTGCTGCTTCTTGTGGGTGAAGGCCATGTCGGGGAAGCTCCCGGCCATGGCGGTCGAGCTAGCAACCGCAACGAAAAGGCTGGCGAGGGACGACCCGAGGAGGGTAGTTCACTCTCTGAAAGTGGGATTAGCCATCACTTTGGTATCTTTGTTTTACTATTTCAGGCCTTTGTATGACGGGTTTGGAGCCTCTACGATGTGGGCCCTCATCTCGGTTATCGTCGTCTTTGAGTTTTCTGTGGGAGGGACGTTGGGGCGTGGTTTGAATAGAGTTATGGCGACACTCGCAGCTGGGGCTCTAGGGTTTGGAGCTCATTACTTGGCGAACTTGGTCGGAGAGAAGGGCCGACCAATAGTGCTTGGTTTCTTTGTGTTCATACCAGCTGCAATATCAACATTCATAAGATTCTTCCCAAAGATGAAGGCAAGATATGATTATGGGATGCTCATATTCATTTTGACATTCTGCATGGTCTCAGTTTCGGGATACAGAGATGATGAGATTGTAAAACTTGCACGTGATAGAGTTTTGACAATTCTCATTGGCTGCTCTTTCACTCTCTTTGTATGTATTTTTGTGCGTCCTGTTTGGGCTGGCTCCGATCTCCACCATTTGGTTGCCACCAACATTGATTCCCTTGCCACCTTCTTACAAGGCTTTGGTGATCAATATTTTGGATTGCTAGGACAAGGACAGGCCAGCCATGGTGACATCCAAATGTATAGATTGATTCTGAACTCGAAATCGAATGAAGAATCATTGACTAACCTAGCAAGATGGGAGCCACGCCATTGGAAGTTCAGATATCGCCATCCTTGGAGGCAATACTTGGAAATTGGAAATTTAAATCGGGACTGCGCCTATCGGCTTGAAGTTCTTAATGGTTACCTTAAAACCAACCTAATTCAAATACCATTATCACAAATCCAAGATCAATTTCAAGACGAATGCTTGAAGATATGCAGCGAATCAGGTAGAAGTTTACGAGAATTGTCATTGGCAATGAAGAAAATGGATCCACCTCTCACTGTCAAACCCCACATGGAGAGAGCAAAAGTTGCAGCTGATAATCTCAAGTCCCATTTGGAAGAATGGAGATTTGAAGACTCAAACGCTATAGAAGTTGTTCGTGTCGCTTCTTTGGTCTCACTTCTCATAGACTCGATATGGTGCGTCGAGAAGATTGGAGACTCAGTTCAAAAATTGGCTTCAGCCGGAGGTTTCAAGGCTGTGGAAGGGCAATCATGTGTAGTAGTGGCACCAGAAGATCAGCTACAAGATTTGGCCAAGGATCATGGAGCCATCCTTTTGGCTCATCATGTCATTACTATTGATGAACAAGAATGTACTCCTTGT

Coding sequence (CDS)

ATGGCATCTCAAAAAACCTCTAATGGGTTTGAAGGGTTACTGCCTTGCTGCTTCTTGTGGGTGAAGGCCATGTCGGGGAAGCTCCCGGCCATGGCGGTCGAGCTAGCAACCGCAACGAAAAGGCTGGCGAGGGACGACCCGAGGAGGGTAGTTCACTCTCTGAAAGTGGGATTAGCCATCACTTTGGTATCTTTGTTTTACTATTTCAGGCCTTTGTATGACGGGTTTGGAGCCTCTACGATGTGGGCCCTCATCTCGGTTATCGTCGTCTTTGAGTTTTCTGTGGGAGGGACGTTGGGGCGTGGTTTGAATAGAGTTATGGCGACACTCGCAGCTGGGGCTCTAGGGTTTGGAGCTCATTACTTGGCGAACTTGGTCGGAGAGAAGGGCCGACCAATAGTGCTTGGTTTCTTTGTGTTCATACCAGCTGCAATATCAACATTCATAAGATTCTTCCCAAAGATGAAGGCAAGATATGATTATGGGATGCTCATATTCATTTTGACATTCTGCATGGTCTCAGTTTCGGGATACAGAGATGATGAGATTGTAAAACTTGCACGTGATAGAGTTTTGACAATTCTCATTGGCTGCTCTTTCACTCTCTTTGTATGTATTTTTGTGCGTCCTGTTTGGGCTGGCTCCGATCTCCACCATTTGGTTGCCACCAACATTGATTCCCTTGCCACCTTCTTACAAGGCTTTGGTGATCAATATTTTGGATTGCTAGGACAAGGACAGGCCAGCCATGGTGACATCCAAATGTATAGATTGATTCTGAACTCGAAATCGAATGAAGAATCATTGACTAACCTAGCAAGATGGGAGCCACGCCATTGGAAGTTCAGATATCGCCATCCTTGGAGGCAATACTTGGAAATTGGAAATTTAAATCGGGACTGCGCCTATCGGCTTGAAGTTCTTAATGGTTACCTTAAAACCAACCTAATTCAAATACCATTATCACAAATCCAAGATCAATTTCAAGACGAATGCTTGAAGATATGCAGCGAATCAGGTAGAAGTTTACGAGAATTGTCATTGGCAATGAAGAAAATGGATCCACCTCTCACTGTCAAACCCCACATGGAGAGAGCAAAAGTTGCAGCTGATAATCTCAAGTCCCATTTGGAAGAATGGAGATTTGAAGACTCAAACGCTATAGAAGTTGTTCGTGTCGCTTCTTTGGTCTCACTTCTCATAGACTCGATATGGTGCGTCGAGAAGATTGGAGACTCAGTTCAAAAATTGGCTTCAGCCGGAGGTTTCAAGGCTGTGGAAGGGCAATCATGTGTAGTAGTGGCACCAGAAGATCAGCTACAAGATTTGGCCAAGGATCATGGAGCCATCCTTTTGGCTCATCATGTCATTACTATTGATGAACAAGAATGTACTCCTTGT

Protein sequence

MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAITLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYFGLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVKPHMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASAGGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC
Homology
BLAST of MC06g1061 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 393.7 bits (1010), Expect = 3.0e-108
Identity = 196/396 (49.49%), Postives = 285/396 (71.97%), Query Frame = 0

Query: 34  ELATATKRLARDDPRRVVHSLKVGLAITLVSLFYYFRPLYDGFGASTMWALISVIVVFEF 93
           E+    +R+ ++DPRRVVH+ KVGLA+ LVS FYY++PLYD FG + MWA+++V+VVFEF
Sbjct: 6   EIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEF 65

Query: 94  SVGGTLGRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFP 153
           SVG TLG+GLNR +ATL AG LG GAH+LA+L G    PI+L  FVF+ AA+STF+RFFP
Sbjct: 66  SVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFP 125

Query: 154 KMKARYDYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWA 213
           ++KARYDYG+LIFILTF ++SVSG+R+DEI+ LA  R+ T+++G    + + IFV PVWA
Sbjct: 126 RVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWA 185

Query: 214 GSDLHHLVATNIDSLATFLQGFGDQYF-----GLLGQGQASHGDIQMYRLILNSKSNEES 273
           G DLH L+A+N D+L+ FLQ FGD+YF     G + + +    +++ Y+ +LNSKSNEE+
Sbjct: 186 GQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSNEEA 245

Query: 274 LTNLARWEPRHWKFRYRHPWRQYLEIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQF 333
           L N A+WEPRH +FR+RHPWRQYL +G L R  AYR++ LN  + +++ QIP+  I+ + 
Sbjct: 246 LANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDM-QIPM-DIKKKI 305

Query: 334 QDECLKICSESGRSLRELSLAMKKMDPPLTVKPHMERAKVAADNLKSHLEEWRFEDSNAI 393
           ++   ++ SESG+S++E+S+++K M    +   H+  ++ A   L + L+     D   +
Sbjct: 306 EEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILNDVEPL 365

Query: 394 EVVRVASLVSLLIDSIWCVEKIGDSVQKLASAGGFK 425
           +++ + + VSLLID +   EKI +SV +LASA  FK
Sbjct: 366 QMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK 399

BLAST of MC06g1061 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 9.9e-104
Identity = 196/423 (46.34%), Postives = 278/423 (65.72%), Query Frame = 0

Query: 34  ELATATKRLARDDPRRVVHSLKVGLAITLVSLFYYFRPLYDGFGASTMWALISVIVVFEF 93
           E+    +R+A++DPRRVVHS KVGL + LVS FYY++PLYD FG + MWA+++V+VVFEF
Sbjct: 6   EIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEF 65

Query: 94  SVGGTLGRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFP 153
           SVG TLG+GLNRV ATL AG LG GAH+LA++ G  G PI+L  FVF+ AA+STF+RFFP
Sbjct: 66  SVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFP 125

Query: 154 KMKARYDYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWA 213
           ++KARYDY +LIFILTF ++SVSG+R++++VKL   R+ T++IG    + + IFV PVWA
Sbjct: 126 RVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWA 185

Query: 214 GSDLHHLVATNIDSLATFLQG--------------------------FGDQYFGLLGQGQ 273
           G DLH L+A+N + L+ FL G                          FGD+Y  ++    
Sbjct: 186 GQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDG 245

Query: 274 ASH-----GDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRDCA 333
           A        D   Y+ +LNSKSNEESL N A+WEP H +FR+RHPW+QYL +G L R CA
Sbjct: 246 AKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCA 305

Query: 334 YRLEVLNGYLKT-NLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVKP 393
           YR+  LN YL   N + +    I+ +  +   ++  ESG++++E+S+++KKM  P +   
Sbjct: 306 YRIHALNSYLNADNQVSV---DIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDL 365

Query: 394 HMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASAG 425
           H++ AK A  +L + L     ++   +E+V + + +SLLID I   EKI +S+ +LA+A 
Sbjct: 366 HVQNAKSACKSLTNLLNSGILKEVEPLELVSLLTAISLLIDIINLTEKILESLHELATAA 425

BLAST of MC06g1061 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 5.6e-99
Identity = 184/392 (46.94%), Postives = 271/392 (69.13%), Query Frame = 0

Query: 41  RLARDDPRRVVHSLKVGLAITLVSLFYY---FRPLYDGFGASTMWALISVIVVFEFSVGG 100
           R+  +DPRR++H+ KVGLA+ LVS FYY   F P  D FG + MWA+++V+VVFEFSVG 
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 101 TLGRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKA 160
           TLG+GLNR +ATL AG LG GAH LA L G    PI+L   VF+ AA+STF+RFFP +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 161 RYDYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDL 220
           ++DYG+LIFILTF ++S+SG+RD+EI+ LA  R+ T++IG    + + IFV PVWAG DL
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 221 HHLVATNIDSLATFLQGFGDQYFGLLGQG-----QASHGDIQMYRLILNSKSNEESLTNL 280
           H L+A+N D+L+ FLQ FGD+YF    +G     +    +++ Y+ +L+SKS+EE+L N 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDSKSDEEALANY 252

Query: 281 ARWEPRHWKFRYRHPWRQYLEIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDEC 340
           A WEP H +FR+RHPW+QY+ +G L R CAYR++ LN Y+ ++  QIP+  I+ + +   
Sbjct: 253 AEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSD-FQIPV-DIKKKLETPL 312

Query: 341 LKICSESGRSLRELSLAMKKMDPPLTVKPHMERAKVAADNLKSHLEEWRFEDSNAIEVVR 400
            ++ SESG S++E+S+++K+M    +   H+  ++ A  +L + L+     D   ++++ 
Sbjct: 313 RRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILNDVEPLQMIS 372

Query: 401 VASLVSLLIDSIWCVEKIGDSVQKLASAGGFK 425
           + + VS+LID +   EKI +SV +LASA  FK
Sbjct: 373 LMTTVSMLIDIVNLTEKISESVHELASAARFK 402

BLAST of MC06g1061 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.5e-96
Identity = 184/387 (47.55%), Postives = 261/387 (67.44%), Query Frame = 0

Query: 40  KRLARDDPRRVVHSLKVGLAITLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTL 99
           ++ A+DDPRR++HS+KVG+A+TLVSL YY RPLY  FG + MWA+++V+VVFEF+VGGTL
Sbjct: 35  QKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTL 94

Query: 100 GRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARY 159
            +GLNR  ATL AGALG GA +LA   G +G PIVLG  VF   A +TF RFFP++K RY
Sbjct: 95  SKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRY 154

Query: 160 DYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHH 219
           DYG LIFILTF  V++SGYR DEI+ +A  R+ TILIG +  + V IF+ PVWAG DLH 
Sbjct: 155 DYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHK 214

Query: 220 LVATNIDSLATFLQGFGDQYFGLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRH 279
           ++A NI+ LA +L+GF  +YF      + +   ++ Y+ IL SKS E+SL NLARWEP H
Sbjct: 215 MIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKSTEDSLANLARWEPGH 274

Query: 280 WKFRYRHPWRQYLEIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSES 339
            +FR RHPW++YL+I  L R CA  LE+LNGY+ +N       + + + Q+    +  E 
Sbjct: 275 GRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSN--DKAPQEFESKIQEPITTMSREV 334

Query: 340 GRSLRELSLAMKKM-DPPLTVKPHMERAKVAADNLKSHLEEWRFED-SNAIEVVRVASLV 399
           G +L+ ++ ++K M +    V  H++ +K A  NLK  L+    E   + +E++   ++ 
Sbjct: 335 GEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYKDLLEIIPGVTMA 394

Query: 400 SLLIDSIWCVEKIGDSVQKLASAGGFK 425
           S+LI+ + CVEKI ++V++ +    FK
Sbjct: 395 SILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of MC06g1061 vs. ExPASy Swiss-Prot
Match: Q76LB1 (Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.6e-85
Identity = 179/433 (41.34%), Postives = 262/433 (60.51%), Query Frame = 0

Query: 12  GLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAITLVSLFYYFRP 71
           G +  C L + ++   L   A   A      AR+DPRRV HSLKVGLA+ LVS+ Y+  P
Sbjct: 15  GTIASCGLLLHSLLAGLGRRAAGFARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTP 74

Query: 72  LYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAHYLANLV---GE 131
           L++G G S +WA+++V+VV E++VG TL +GLNR +ATL AG +  GAH LA L    G+
Sbjct: 75  LFNGLGVSAIWAVLTVVVVMEYTVGATLSKGLNRALATLVAGCIAVGAHQLAELAERCGD 134

Query: 132 KGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRDDEIVKLAR 191
           +G PIVL   VF  A+ +TF+RF P++KA+YDYG+ IFILTF +V+VS YR +E+++LA 
Sbjct: 135 QGEPIVLTVLVFFVASAATFLRFIPEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAH 194

Query: 192 DRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYFG--LLGQG 251
            R  TI +G    L   +F+ PVWAG D+H L + N+D LA F++G     FG   +   
Sbjct: 195 QRFYTIAVGVFICLCTTVFLFPVWAGEDVHKLASGNLDKLAQFIEGMEFNCFGENSVANN 254

Query: 252 QASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRDCAYRLE 311
                  QM++ +LNSK+ E+SL   A+WEPRH +FR+RHPW QY ++G L R CA  +E
Sbjct: 255 FGGKDSPQMHKSVLNSKATEDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSME 314

Query: 312 VLNGY-LKTNLIQIPLS---QIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVKPH 371
            L  Y + T+  Q P +   ++  + +  C ++   S + LR+L++A + M  P  V   
Sbjct: 315 ALASYVITTSKTQCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNIT 374

Query: 372 MERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASAGG 431
           M  A  AA++L+S L     E++  ++V+ VA   +LL D +  V++I + V  LA    
Sbjct: 375 MATAVKAAESLRSELA----ENTALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAH 434

Query: 432 FKAVEGQSCVVVA 436
           FK  E    VVV+
Sbjct: 435 FKNPEDTKNVVVS 443

BLAST of MC06g1061 vs. NCBI nr
Match: XP_022153275.1 (aluminum-activated malate transporter 2-like [Momordica charantia])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI
Sbjct: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH
Sbjct: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD
Sbjct: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF
Sbjct: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240

Query: 241 GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD 300
           GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD
Sbjct: 241 GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD 300

Query: 301 CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK 360
           CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK
Sbjct: 301 CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK 360

Query: 361 PHMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASA 420
           PHMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASA
Sbjct: 361 PHMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASA 420

Query: 421 GGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467
           GGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC
Sbjct: 421 GGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467

BLAST of MC06g1061 vs. NCBI nr
Match: XP_038880572.1 (aluminum-activated malate transporter 2-like [Benincasa hispida])

HSP 1 Score: 674 bits (1740), Expect = 2.63e-240
Identity = 344/472 (72.88%), Postives = 391/472 (82.84%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K S+G+EGLLP C  WVK +  KLPAMAV+LA       +DDPRRVVH+LKVGLAI
Sbjct: 1   MATEKNSDGYEGLLPLCLRWVKPVFAKLPAMAVKLA-------KDDPRRVVHALKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVS FYYF+PLYDGFG STMWA+++VIVVFEFSVGGTLGRGLNRVMAT  AGALGFGAH
Sbjct: 61  TLVSFFYYFKPLYDGFGTSTMWAIVTVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L GE GRPI+LG FVF+   ISTFIRFFPK+KARYDYGMLIFILTFCMVS+SGYRD
Sbjct: 121 YLASLGGETGRPIMLGLFVFVLGGISTFIRFFPKIKARYDYGMLIFILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEI KLA  R+LTILIGC FT+ VCIFVRPVWAG+DLH+LVATNI SLA F +GFG +YF
Sbjct: 181 DEIAKLAISRILTILIGCCFTVIVCIFVRPVWAGTDLHYLVATNIQSLAIFFEGFGVEYF 240

Query: 241 GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD 300
           GL  +G+ S  D+Q YR ILNSKSNEESLTNLARWEPRH KFRYRHPW+QYL IG+LNR+
Sbjct: 241 GLSKEGEVSKTDMQKYRTILNSKSNEESLTNLARWEPRHGKFRYRHPWKQYLVIGSLNRE 300

Query: 301 CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK 360
           CAYRLEVLNG LK N  Q+P SQ+  QFQ+EC KICSES R LRELSLA++KM PPLT K
Sbjct: 301 CAYRLEVLNGCLKANQFQMP-SQVHGQFQEECRKICSESSRGLRELSLALRKMVPPLTAK 360

Query: 361 PHMERAKVAADNLKSHLEEWRFED-SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLAS 420
            H+E+AK+AA+NLKSHLEEWRFE+ +NA+E+V V SL SLL D+I C+EKI DSVQKLAS
Sbjct: 361 AHIEKAKIAAENLKSHLEEWRFEEVNNAMEIVHVISLTSLLFDTICCIEKIVDSVQKLAS 420

Query: 421 AGGFKAVEGQSCVVVAPEDQLQD---LAKDHGA-ILLAHHVITIDEQECTPC 467
             GFKAVE QS V    ++ LQD   L   HG  ILLAHHVITIDE+  TPC
Sbjct: 421 MAGFKAVEVQSGVAPQQQNDLQDEGSLQPCHGTTILLAHHVITIDEE--TPC 462

BLAST of MC06g1061 vs. NCBI nr
Match: XP_022988102.1 (aluminum-activated malate transporter 2-like [Cucurbita maxima])

HSP 1 Score: 671 bits (1731), Expect = 7.65e-239
Identity = 336/472 (71.19%), Postives = 390/472 (82.63%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K+SN +EGLLP C  WVK +  KLPAMAVELAT TK+LA+DD RRVVH+LKVGLAI
Sbjct: 1   MATEKSSNEYEGLLPFCLRWVKPIFTKLPAMAVELATMTKKLAKDDTRRVVHALKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVSLFYYF+PLYDGFG ST+WA+++V+VVFEFSVGGTLGRGLNRVMATL AGALGFGAH
Sbjct: 61  TLVSLFYYFKPLYDGFGTSTIWAVVTVVVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L G+ GRPI+LG FVF+ AAISTFIRFFPKMKARYDYG+LIFILTFCMVS+SGYRD
Sbjct: 121 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEI KLA  R+LTILIGC FT+FVC+FV PVWAG+DLHHLVA+NIDSLATF  GFG +YF
Sbjct: 181 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVASNIDSLATFFHGFGAEYF 240

Query: 241 GLLG-QGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNR 300
           GLL  QG+    D+Q YR +LNSKSNEESLTNLARWEPRH KFRYRHPW+ YLEIG+LNR
Sbjct: 241 GLLQEQGEVGKVDMQKYRTVLNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 300

Query: 301 DCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTV 360
           +CA RLEVLNGYL+T   Q+P  QI   FQ+EC+KICSE    LREL++ ++KM PPLT 
Sbjct: 301 ECACRLEVLNGYLQTKRTQMPSQQIHGPFQEECMKICSELSNGLRELAMGLRKMVPPLTA 360

Query: 361 KPHMERAKVAADNLKSHLEEWRFED----SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQ 420
           K H+E+AK+AA+NLKSHLEEWRFE+     NA+E+V VASL  LL+D+I C+ KI DSVQ
Sbjct: 361 KAHIEKAKIAAENLKSHLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIADSVQ 420

Query: 421 KLASAGGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467
           KLAS  GFKAVE  S V  APE     +   HGA+L+AH VI ID+    PC
Sbjct: 421 KLASIAGFKAVEVPSSV--APEQDQGSMQPCHGAVLVAHQVIMIDKG--APC 468

BLAST of MC06g1061 vs. NCBI nr
Match: XP_022960622.1 (aluminum-activated malate transporter 2-like [Cucurbita moschata])

HSP 1 Score: 671 bits (1731), Expect = 7.65e-239
Identity = 337/472 (71.40%), Postives = 390/472 (82.63%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K+SN +EGLLP C  WVK +  KLPA+AVELAT T +LA+DD RRVVH+LKVGLAI
Sbjct: 1   MATEKSSNEYEGLLPFCLRWVKPIFAKLPAIAVELATMTTKLAKDDTRRVVHALKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVSLFYYF+PLYDGFG STMWA+++VIVVFEFSVGGTLGRGLNRVMATL AGALGFGAH
Sbjct: 61  TLVSLFYYFKPLYDGFGTSTMWAVVTVIVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L G+ GRPI+LG FVF+ AAISTFIRFFPKMKARYDYG+LIFILTFCMVS+SGYRD
Sbjct: 121 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEI KLA  R+LTILIGC FT+FVC+FV PVWAG+DLHHLVA NI+SLATF  GFG +YF
Sbjct: 181 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVANNIESLATFFHGFGAEYF 240

Query: 241 GLLG-QGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNR 300
           GLL  QG+    D+Q YR ILNSKSNEESLTNLARWEPRH KFRYRHPW+ YLEIG+LNR
Sbjct: 241 GLLQEQGEVGKVDMQKYRTILNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 300

Query: 301 DCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTV 360
           +CA RLEVLNGYL+T   Q+P  QI   FQ+EC+KICSE    LREL++ ++KM PPLT 
Sbjct: 301 ECACRLEVLNGYLQTKRTQMPSQQIHGPFQEECMKICSELSSGLRELAMGLRKMVPPLTA 360

Query: 361 KPHMERAKVAADNLKSHLEEWRFED----SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQ 420
           K H+E+AK+AA+NLKSHLEEWRFE+     NA+E+V VASL  LL+D+I C+ KI DSVQ
Sbjct: 361 KAHIEKAKIAAENLKSHLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIVDSVQ 420

Query: 421 KLASAGGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467
           KLAS  GFKAVE  S V  APE     +   HG +L+AHHVI IDE+  +PC
Sbjct: 421 KLASIAGFKAVEVPSSV--APEQDQGSMQPCHGDVLVAHHVIMIDEE--SPC 468

BLAST of MC06g1061 vs. NCBI nr
Match: XP_023516927.1 (aluminum-activated malate transporter 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670 bits (1729), Expect = 1.54e-238
Identity = 337/472 (71.40%), Postives = 389/472 (82.42%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K+SN +EGLLP C  WVK +  KLPAMAVELAT T +LA+DD RRVVH+LKVGLAI
Sbjct: 1   MATEKSSNEYEGLLPFCLRWVKPIFAKLPAMAVELATMTTKLAKDDTRRVVHALKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVSLFYYF+PLYDGFG STMWA+++VIVVFEFSVGGTLGRGLNRVMATL AGALGFGAH
Sbjct: 61  TLVSLFYYFKPLYDGFGTSTMWAVVTVIVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L G+ GRPI+LG FVF+ AAISTFIRFFPKMKARYDYG+LIFILTFCMVS+SGYRD
Sbjct: 121 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEI KLA  R+LTILIGC FT+FVC+FV PVWAG+DLHHLVA NI+SLATF  GFG +YF
Sbjct: 181 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVANNIESLATFFHGFGAEYF 240

Query: 241 GLLG-QGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNR 300
           GLL  QG+    D+Q YR ILNSKSNEESLTNLARWEPRH KFRYRHPW+ YLEIG+LNR
Sbjct: 241 GLLQEQGEVGKVDMQKYRTILNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 300

Query: 301 DCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTV 360
           +CA RLEVLNGYL+T   Q+P  QI   FQ+EC+KICSE    LREL++ ++KM PPLT 
Sbjct: 301 ECACRLEVLNGYLQTKRTQMPSQQIHGPFQEECMKICSELSNGLRELAMGLRKMVPPLTA 360

Query: 361 KPHMERAKVAADNLKSHLEEWRFED----SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQ 420
           K H+E+AK+AA+NLKSHLEEWRFE+    +NA+E+V VASL  LL+D+I C+ KI DSVQ
Sbjct: 361 KAHIEKAKIAAENLKSHLEEWRFEEMNNNNNALEIVHVASLSPLLLDTICCIVKIVDSVQ 420

Query: 421 KLASAGGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467
           KLAS  GFKAVE  S V  APE     +   HG +L+AHHVI I E+   PC
Sbjct: 421 KLASIAGFKAVEVPSSV--APEQDQGSMQPCHGDVLVAHHVIMIHEE--APC 468

BLAST of MC06g1061 vs. ExPASy TrEMBL
Match: A0A6J1DGC6 (aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=LOC111020801 PE=3 SV=1)

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI
Sbjct: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH
Sbjct: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD
Sbjct: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF
Sbjct: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240

Query: 241 GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD 300
           GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD
Sbjct: 241 GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD 300

Query: 301 CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK 360
           CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK
Sbjct: 301 CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK 360

Query: 361 PHMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASA 420
           PHMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASA
Sbjct: 361 PHMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASA 420

Query: 421 GGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467
           GGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC
Sbjct: 421 GGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467

BLAST of MC06g1061 vs. ExPASy TrEMBL
Match: A0A6J1JC53 (aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485449 PE=3 SV=1)

HSP 1 Score: 671 bits (1731), Expect = 3.70e-239
Identity = 336/472 (71.19%), Postives = 390/472 (82.63%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K+SN +EGLLP C  WVK +  KLPAMAVELAT TK+LA+DD RRVVH+LKVGLAI
Sbjct: 1   MATEKSSNEYEGLLPFCLRWVKPIFTKLPAMAVELATMTKKLAKDDTRRVVHALKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVSLFYYF+PLYDGFG ST+WA+++V+VVFEFSVGGTLGRGLNRVMATL AGALGFGAH
Sbjct: 61  TLVSLFYYFKPLYDGFGTSTIWAVVTVVVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L G+ GRPI+LG FVF+ AAISTFIRFFPKMKARYDYG+LIFILTFCMVS+SGYRD
Sbjct: 121 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEI KLA  R+LTILIGC FT+FVC+FV PVWAG+DLHHLVA+NIDSLATF  GFG +YF
Sbjct: 181 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVASNIDSLATFFHGFGAEYF 240

Query: 241 GLLG-QGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNR 300
           GLL  QG+    D+Q YR +LNSKSNEESLTNLARWEPRH KFRYRHPW+ YLEIG+LNR
Sbjct: 241 GLLQEQGEVGKVDMQKYRTVLNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 300

Query: 301 DCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTV 360
           +CA RLEVLNGYL+T   Q+P  QI   FQ+EC+KICSE    LREL++ ++KM PPLT 
Sbjct: 301 ECACRLEVLNGYLQTKRTQMPSQQIHGPFQEECMKICSELSNGLRELAMGLRKMVPPLTA 360

Query: 361 KPHMERAKVAADNLKSHLEEWRFED----SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQ 420
           K H+E+AK+AA+NLKSHLEEWRFE+     NA+E+V VASL  LL+D+I C+ KI DSVQ
Sbjct: 361 KAHIEKAKIAAENLKSHLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIADSVQ 420

Query: 421 KLASAGGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467
           KLAS  GFKAVE  S V  APE     +   HGA+L+AH VI ID+    PC
Sbjct: 421 KLASIAGFKAVEVPSSV--APEQDQGSMQPCHGAVLVAHQVIMIDKG--APC 468

BLAST of MC06g1061 vs. ExPASy TrEMBL
Match: A0A6J1H7Y1 (aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461353 PE=3 SV=1)

HSP 1 Score: 671 bits (1731), Expect = 3.70e-239
Identity = 337/472 (71.40%), Postives = 390/472 (82.63%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K+SN +EGLLP C  WVK +  KLPA+AVELAT T +LA+DD RRVVH+LKVGLAI
Sbjct: 1   MATEKSSNEYEGLLPFCLRWVKPIFAKLPAIAVELATMTTKLAKDDTRRVVHALKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           TLVSLFYYF+PLYDGFG STMWA+++VIVVFEFSVGGTLGRGLNRVMATL AGALGFGAH
Sbjct: 61  TLVSLFYYFKPLYDGFGTSTMWAVVTVIVVFEFSVGGTLGRGLNRVMATLLAGALGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L G+ GRPI+LG FVF+ AAISTFIRFFPKMKARYDYG+LIFILTFCMVS+SGYRD
Sbjct: 121 YLASLGGDTGRPIMLGLFVFVLAAISTFIRFFPKMKARYDYGLLIFILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEI KLA  R+LTILIGC FT+FVC+FV PVWAG+DLHHLVA NI+SLATF  GFG +YF
Sbjct: 181 DEIAKLAYSRMLTILIGCCFTVFVCVFVCPVWAGTDLHHLVANNIESLATFFHGFGAEYF 240

Query: 241 GLLG-QGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNR 300
           GLL  QG+    D+Q YR ILNSKSNEESLTNLARWEPRH KFRYRHPW+ YLEIG+LNR
Sbjct: 241 GLLQEQGEVGKVDMQKYRTILNSKSNEESLTNLARWEPRHGKFRYRHPWKVYLEIGSLNR 300

Query: 301 DCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTV 360
           +CA RLEVLNGYL+T   Q+P  QI   FQ+EC+KICSE    LREL++ ++KM PPLT 
Sbjct: 301 ECACRLEVLNGYLQTKRTQMPSQQIHGPFQEECMKICSELSSGLRELAMGLRKMVPPLTA 360

Query: 361 KPHMERAKVAADNLKSHLEEWRFED----SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQ 420
           K H+E+AK+AA+NLKSHLEEWRFE+     NA+E+V VASL  LL+D+I C+ KI DSVQ
Sbjct: 361 KAHIEKAKIAAENLKSHLEEWRFEEMNNNKNALEIVHVASLSPLLLDTICCIVKIVDSVQ 420

Query: 421 KLASAGGFKAVEGQSCVVVAPEDQLQDLAKDHGAILLAHHVITIDEQECTPC 467
           KLAS  GFKAVE  S V  APE     +   HG +L+AHHVI IDE+  +PC
Sbjct: 421 KLASIAGFKAVEVPSSV--APEQDQGSMQPCHGDVLVAHHVIMIDEE--SPC 468

BLAST of MC06g1061 vs. ExPASy TrEMBL
Match: A0A0A0M301 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630850 PE=3 SV=1)

HSP 1 Score: 662 bits (1709), Expect = 9.87e-236
Identity = 332/477 (69.60%), Postives = 393/477 (82.39%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K SNG+EGLLP C  W+K +  KL  ++V+LAT  K+LA+DD RRVVH+LKVGLAI
Sbjct: 1   MATEKNSNGYEGLLPLCLRWLKPIFAKLSTVSVKLATMAKKLAKDDSRRVVHALKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           +LVSL YYF+PLYDGFG STMWA+++VIVVFEFSVGGTLGRGLNRVMATL AG LGFG H
Sbjct: 61  SLVSLLYYFKPLYDGFGTSTMWAIVTVIVVFEFSVGGTLGRGLNRVMATLLAGGLGFGTH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L G+ GRPI+L  FVFI A++STF RFFPK+KARYDYG+LI ILTFCMVS+SGYRD
Sbjct: 121 YLASLGGDTGRPIILALFVFILASVSTFTRFFPKIKARYDYGLLILILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           +EI KLA  R+LTILIGC  TL VCIFVRPVWAG+DLH LVA NI SLA F QGFG ++F
Sbjct: 181 EEIAKLALSRILTILIGCCVTLIVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFF 240

Query: 241 GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD 300
           GL  +G+ S+ D+Q YR ILNSKSNEESLTNLARWEPRH KFRYRHPW+QYL+IG+LNR+
Sbjct: 241 GLSQEGEVSNDDMQKYRTILNSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRE 300

Query: 301 CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK 360
           CAYRLE+LNGYLKTN  Q+P  QI  QF++EC+KICSES R LREL+LA++KM  PLT K
Sbjct: 301 CAYRLELLNGYLKTNQFQMPSQQIHGQFKEECMKICSESSRGLRELALALRKMVLPLTAK 360

Query: 361 PHMERAKVAADNLKSHLEEWRFED-SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLAS 420
            H+E+AK+AA+NLKSHLEEWRFE+ +NA+E+V+V SL SLL D+I C+EKI DSVQ+LAS
Sbjct: 361 SHIEKAKIAAENLKSHLEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELAS 420

Query: 421 AGGFKAVEGQSCVVVAPEDQLQDLAKD--------HGA-ILLAHHVITIDEQECTPC 467
             GFKAVE QS V  APE Q+    +D        HGA +LLAHH ITIDEQ  +PC
Sbjct: 421 MAGFKAVEVQSSV--APEQQMDLQDQDQYALQPLSHGAAVLLAHHAITIDEQ--SPC 473

BLAST of MC06g1061 vs. ExPASy TrEMBL
Match: A0A1S3BNX0 (aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC103491947 PE=3 SV=1)

HSP 1 Score: 660 bits (1703), Expect = 7.50e-235
Identity = 339/476 (71.22%), Postives = 392/476 (82.35%), Query Frame = 0

Query: 1   MASQKTSNGFEGLLPCCFLWVKAMSGKLPAMAVELATATKRLARDDPRRVVHSLKVGLAI 60
           MA++K SNG+EGLLP C  W+K +  KL   AV+LAT TK+LA+DD RRVVH+ KVGLAI
Sbjct: 1   MATEKNSNGYEGLLPLCLRWLKPIFAKLSNKAVKLATMTKKLAKDDSRRVVHAFKVGLAI 60

Query: 61  TLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAH 120
           +LVSL YYF+ LYDGFG STMWA+++VIVV EFSVGGTLGRGLNRVMATL AG LGFGAH
Sbjct: 61  SLVSLLYYFKALYDGFGTSTMWAIVTVIVVVEFSVGGTLGRGLNRVMATLLAGGLGFGAH 120

Query: 121 YLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRD 180
           YLA+L G+ GRPI+L FFVF+ A+ISTF RFFPK+KARYDYGMLIFILTFCMVS+SGYRD
Sbjct: 121 YLASLSGDTGRPIILAFFVFLLASISTFTRFFPKIKARYDYGMLIFILTFCMVSLSGYRD 180

Query: 181 DEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF 240
           DEI KLA  R+LTILIGC FTLFVCIFVRPVWAG+DLH LVA NI SLA F QGFG ++F
Sbjct: 181 DEIAKLALSRILTILIGCCFTLFVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFF 240

Query: 241 GLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRD 300
            L  Q + S  D+Q YR ILNSKSNEESLTNLARWEPRH KFRYRHPW+QYL+IG+LNR+
Sbjct: 241 RL-SQEEVSKDDMQKYRTILNSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRE 300

Query: 301 CAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVK 360
           CAYRLE+LNGYLKTN  Q+P  QI   F++EC+KICSES R LREL+LA++KM  PLT K
Sbjct: 301 CAYRLELLNGYLKTNQFQMPSQQIHGPFKEECMKICSESSRGLRELALALRKMVLPLTAK 360

Query: 361 PHMERAKVAADNLKSHLEEWRFED-SNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLAS 420
            H+E+AKVAA++LKSHLEEWRFE+ +NA+E+V+V SL SLL D+I C+EKI DSVQ+LAS
Sbjct: 361 SHIEKAKVAAEDLKSHLEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELAS 420

Query: 421 AGGFKAVEGQSCVVVAPEDQ--LQD-----LAKDHGA-ILLAHHVITIDEQECTPC 467
             GFKAVE QS V  APE Q  LQD     L   HGA +LLAHH ITIDEQ  +PC
Sbjct: 421 MAGFKAVEVQSSV--APEQQMDLQDQDQYSLQPSHGAAVLLAHHAITIDEQ--SPC 471

BLAST of MC06g1061 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 393.7 bits (1010), Expect = 2.1e-109
Identity = 196/396 (49.49%), Postives = 285/396 (71.97%), Query Frame = 0

Query: 34  ELATATKRLARDDPRRVVHSLKVGLAITLVSLFYYFRPLYDGFGASTMWALISVIVVFEF 93
           E+    +R+ ++DPRRVVH+ KVGLA+ LVS FYY++PLYD FG + MWA+++V+VVFEF
Sbjct: 6   EIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEF 65

Query: 94  SVGGTLGRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFP 153
           SVG TLG+GLNR +ATL AG LG GAH+LA+L G    PI+L  FVF+ AA+STF+RFFP
Sbjct: 66  SVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFP 125

Query: 154 KMKARYDYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWA 213
           ++KARYDYG+LIFILTF ++SVSG+R+DEI+ LA  R+ T+++G    + + IFV PVWA
Sbjct: 126 RVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWA 185

Query: 214 GSDLHHLVATNIDSLATFLQGFGDQYF-----GLLGQGQASHGDIQMYRLILNSKSNEES 273
           G DLH L+A+N D+L+ FLQ FGD+YF     G + + +    +++ Y+ +LNSKSNEE+
Sbjct: 186 GQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSNEEA 245

Query: 274 LTNLARWEPRHWKFRYRHPWRQYLEIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQF 333
           L N A+WEPRH +FR+RHPWRQYL +G L R  AYR++ LN  + +++ QIP+  I+ + 
Sbjct: 246 LANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDM-QIPM-DIKKKI 305

Query: 334 QDECLKICSESGRSLRELSLAMKKMDPPLTVKPHMERAKVAADNLKSHLEEWRFEDSNAI 393
           ++   ++ SESG+S++E+S+++K M    +   H+  ++ A   L + L+     D   +
Sbjct: 306 EEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILNDVEPL 365

Query: 394 EVVRVASLVSLLIDSIWCVEKIGDSVQKLASAGGFK 425
           +++ + + VSLLID +   EKI +SV +LASA  FK
Sbjct: 366 QMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK 399

BLAST of MC06g1061 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 378.6 bits (971), Expect = 7.1e-105
Identity = 196/423 (46.34%), Postives = 278/423 (65.72%), Query Frame = 0

Query: 34  ELATATKRLARDDPRRVVHSLKVGLAITLVSLFYYFRPLYDGFGASTMWALISVIVVFEF 93
           E+    +R+A++DPRRVVHS KVGL + LVS FYY++PLYD FG + MWA+++V+VVFEF
Sbjct: 6   EIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEF 65

Query: 94  SVGGTLGRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFP 153
           SVG TLG+GLNRV ATL AG LG GAH+LA++ G  G PI+L  FVF+ AA+STF+RFFP
Sbjct: 66  SVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFP 125

Query: 154 KMKARYDYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWA 213
           ++KARYDY +LIFILTF ++SVSG+R++++VKL   R+ T++IG    + + IFV PVWA
Sbjct: 126 RVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWA 185

Query: 214 GSDLHHLVATNIDSLATFLQG--------------------------FGDQYFGLLGQGQ 273
           G DLH L+A+N + L+ FL G                          FGD+Y  ++    
Sbjct: 186 GQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDG 245

Query: 274 ASH-----GDIQMYRLILNSKSNEESLTNLARWEPRHWKFRYRHPWRQYLEIGNLNRDCA 333
           A        D   Y+ +LNSKSNEESL N A+WEP H +FR+RHPW+QYL +G L R CA
Sbjct: 246 AKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCA 305

Query: 334 YRLEVLNGYLKT-NLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMKKMDPPLTVKP 393
           YR+  LN YL   N + +    I+ +  +   ++  ESG++++E+S+++KKM  P +   
Sbjct: 306 YRIHALNSYLNADNQVSV---DIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDL 365

Query: 394 HMERAKVAADNLKSHLEEWRFEDSNAIEVVRVASLVSLLIDSIWCVEKIGDSVQKLASAG 425
           H++ AK A  +L + L     ++   +E+V + + +SLLID I   EKI +S+ +LA+A 
Sbjct: 366 HVQNAKSACKSLTNLLNSGILKEVEPLELVSLLTAISLLIDIINLTEKILESLHELATAA 425

BLAST of MC06g1061 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 362.8 bits (930), Expect = 4.0e-100
Identity = 184/392 (46.94%), Postives = 271/392 (69.13%), Query Frame = 0

Query: 41  RLARDDPRRVVHSLKVGLAITLVSLFYY---FRPLYDGFGASTMWALISVIVVFEFSVGG 100
           R+  +DPRR++H+ KVGLA+ LVS FYY   F P  D FG + MWA+++V+VVFEFSVG 
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 101 TLGRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKA 160
           TLG+GLNR +ATL AG LG GAH LA L G    PI+L   VF+ AA+STF+RFFP +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 161 RYDYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDL 220
           ++DYG+LIFILTF ++S+SG+RD+EI+ LA  R+ T++IG    + + IFV PVWAG DL
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 221 HHLVATNIDSLATFLQGFGDQYFGLLGQG-----QASHGDIQMYRLILNSKSNEESLTNL 280
           H L+A+N D+L+ FLQ FGD+YF    +G     +    +++ Y+ +L+SKS+EE+L N 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDSKSDEEALANY 252

Query: 281 ARWEPRHWKFRYRHPWRQYLEIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDEC 340
           A WEP H +FR+RHPW+QY+ +G L R CAYR++ LN Y+ ++  QIP+  I+ + +   
Sbjct: 253 AEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSD-FQIPV-DIKKKLETPL 312

Query: 341 LKICSESGRSLRELSLAMKKMDPPLTVKPHMERAKVAADNLKSHLEEWRFEDSNAIEVVR 400
            ++ SESG S++E+S+++K+M    +   H+  ++ A  +L + L+     D   ++++ 
Sbjct: 313 RRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILNDVEPLQMIS 372

Query: 401 VASLVSLLIDSIWCVEKIGDSVQKLASAGGFK 425
           + + VS+LID +   EKI +SV +LASA  FK
Sbjct: 373 LMTTVSMLIDIVNLTEKISESVHELASAARFK 402

BLAST of MC06g1061 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-97
Identity = 184/387 (47.55%), Postives = 261/387 (67.44%), Query Frame = 0

Query: 40  KRLARDDPRRVVHSLKVGLAITLVSLFYYFRPLYDGFGASTMWALISVIVVFEFSVGGTL 99
           ++ A+DDPRR++HS+KVG+A+TLVSL YY RPLY  FG + MWA+++V+VVFEF+VGGTL
Sbjct: 35  QKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTL 94

Query: 100 GRGLNRVMATLAAGALGFGAHYLANLVGEKGRPIVLGFFVFIPAAISTFIRFFPKMKARY 159
            +GLNR  ATL AGALG GA +LA   G +G PIVLG  VF   A +TF RFFP++K RY
Sbjct: 95  SKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRY 154

Query: 160 DYGMLIFILTFCMVSVSGYRDDEIVKLARDRVLTILIGCSFTLFVCIFVRPVWAGSDLHH 219
           DYG LIFILTF  V++SGYR DEI+ +A  R+ TILIG +  + V IF+ PVWAG DLH 
Sbjct: 155 DYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHK 214

Query: 220 LVATNIDSLATFLQGFGDQYFGLLGQGQASHGDIQMYRLILNSKSNEESLTNLARWEPRH 279
           ++A NI+ LA +L+GF  +YF      + +   ++ Y+ IL SKS E+SL NLARWEP H
Sbjct: 215 MIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKSTEDSLANLARWEPGH 274

Query: 280 WKFRYRHPWRQYLEIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSES 339
            +FR RHPW++YL+I  L R CA  LE+LNGY+ +N       + + + Q+    +  E 
Sbjct: 275 GRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSN--DKAPQEFESKIQEPITTMSREV 334

Query: 340 GRSLRELSLAMKKM-DPPLTVKPHMERAKVAADNLKSHLEEWRFED-SNAIEVVRVASLV 399
           G +L+ ++ ++K M +    V  H++ +K A  NLK  L+    E   + +E++   ++ 
Sbjct: 335 GEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYKDLLEIIPGVTMA 394

Query: 400 SLLIDSIWCVEKIGDSVQKLASAGGFK 425
           S+LI+ + CVEKI ++V++ +    FK
Sbjct: 395 SILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of MC06g1061 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-76
Identity = 178/458 (38.86%), Postives = 258/458 (56.33%), Query Frame = 0

Query: 12  GLLPCCFLWVKAMSGK-LPAMAVELATATKRLARDDPRRVVHSLKVGLAITLVSLFYYFR 71
           GL    FLW+K +  K +     +      R+  DDP +VVH LKVGLA++LVS+FYY R
Sbjct: 28  GLSKRIFLWLKDLVMKVIMERVAKFMRKAWRIGADDPAKVVHCLKVGLALSLVSIFYYMR 87

Query: 72  PLYDGFGASTMWALISVIVVFEFSVGGTLGRGLNRVMATLAAGALGFGAHYLANLVGEKG 131
           PLYDG G + MWA+++V+VVFE +VG T  + +NRV+AT+ AG+LG   H++A   G K 
Sbjct: 88  PLYDGVGGNAMWAIMTVVVVFESNVGATFCKCVNRVVATILAGSLGIAVHWVATQSG-KA 147

Query: 132 RPIVLGFFVFIPAAISTFIRFFPKMKARYDYGMLIFILTFCMVSVSGYRDDEIVKLARDR 191
              V+G  VF+ A  +T+ RF P  KAR+DYG +IFILTF +VSV GYR D++V+LA+ R
Sbjct: 148 EVFVIGCSVFLFAFAATYSRFVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQR 207

Query: 192 VLTILIGCSFTLFVCIFVRPVWAGSDLHHLVATNIDSLATFLQGFGDQYF------GLLG 251
           V TI IG S  + + +F  P+WAGS LH L+  N++ LA  L G   +YF          
Sbjct: 208 VSTIAIGTSICIIITVFFCPIWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRN 267

Query: 252 QGQASHGDIQMYRLILNSKSNEESL------------TNLARWEPRHWKFRYRHPWRQYL 311
           + + ++  +Q ++ +LNSK  EE++             NLARWEP H  F +RHPW+ Y+
Sbjct: 268 EDENTNMKLQGFKCVLNSKGTEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYV 327

Query: 312 EIGNLNRDCAYRLEVLNGYLKTNLIQIPLSQIQDQFQDECLKICSESGRSLRELSLAMK- 371
           +IG   R CAY LE L+  +  N       Q+++ F + C+K+ S S + LREL+  MK 
Sbjct: 328 KIGAAMRRCAYCLENLS--ICINYETEAPDQVKNHFGEACMKLSSASSKILRELADMMKN 387

Query: 372 -----KMD--------------------PPLTVKPHMERAKVAADNLKSHLEEWRFEDSN 425
                KMD                    P  T KP     +V ++       E R    +
Sbjct: 388 TRKSSKMDFLVFDMNSAVQELQETLKNVPIETNKPE----EVPSEEENKVDSEERTTSMS 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJE83.0e-10849.49Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9XIN19.9e-10446.34Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Q9SJE95.6e-9946.94Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q9SRM91.5e-9647.55Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q76LB13.6e-8541.34Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE... [more]
Match NameE-valueIdentityDescription
XP_022153275.10.0100.00aluminum-activated malate transporter 2-like [Momordica charantia][more]
XP_038880572.12.63e-24072.88aluminum-activated malate transporter 2-like [Benincasa hispida][more]
XP_022988102.17.65e-23971.19aluminum-activated malate transporter 2-like [Cucurbita maxima][more]
XP_022960622.17.65e-23971.40aluminum-activated malate transporter 2-like [Cucurbita moschata][more]
XP_023516927.11.54e-23871.40aluminum-activated malate transporter 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DGC60.0100.00aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1JC533.70e-23971.19aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1H7Y13.70e-23971.40aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0M3019.87e-23669.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630850 PE=3 SV=1[more]
A0A1S3BNX07.50e-23571.22aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC10349... [more]
Match NameE-valueIdentityDescription
AT1G08440.12.1e-10949.49Aluminium activated malate transporter family protein [more]
AT2G27240.17.1e-10546.34Aluminium activated malate transporter family protein [more]
AT1G08430.14.0e-10046.94aluminum-activated malate transporter 1 [more]
AT3G11680.11.1e-9747.55Aluminium activated malate transporter family protein [more]
AT4G00910.11.1e-7638.86Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 363..383
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 21..430
NoneNo IPR availablePANTHERPTHR31086:SF53ALUMINUM ACTIVATED MALATE TRANSPORTER FAMILY PROTEINcoord: 21..430
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 41..379
e-value: 8.6E-135
score: 450.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1061.1MC06g1061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane