MC06g0978 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0978
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncyclin-dependent protein kinase inhibitor SMR9-like
LocationMC06: 8735716 .. 8736090 (+)
RNA-Seq ExpressionMC06g0978
SyntenyMC06g0978
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCAAAGGAGGAATAGCAAGAACAAAGATGAAGATGAGCAAGAAGATCAAGAAGAAGAGCCCACAGAGGATGAAGAAGAGAAATCCTCCTCCTCCTTCTCCTCCTCCAAAGAATCAGGAAACCGAAACCGAAACCGCAGTCGAATTAGCTTCAAGCTCAGCAGCCATGGCGGAAAATGAGAGCATGAGCAGTGATTTGGAAGAGTGCTGCACTCCAAAAGCAGAGAGATTCAGAATCCCAGAGATCAAAATCTGCCCTCCAGCTCCAAAAAAACAGAGGATTTTCTCCAATTGTTCTAATTTACAGAGACCTTCTCCAATTGCCTTCTTTGCTTCTCCAGATATAGAGCTCTTCTTCTTCTTCTCCTCC

mRNA sequence

ATGGCTTCCAAAGGAGGAATAGCAAGAACAAAGATGAAGATGAGCAAGAAGATCAAGAAGAAGAGCCCACAGAGGATGAAGAAGAGAAATCCTCCTCCTCCTTCTCCTCCTCCAAAGAATCAGGAAACCGAAACCGAAACCGCAGTCGAATTAGCTTCAAGCTCAGCAGCCATGGCGGAAAATGAGAGCATGAGCAGTGATTTGGAAGAGTGCTGCACTCCAAAAGCAGAGAGATTCAGAATCCCAGAGATCAAAATCTGCCCTCCAGCTCCAAAAAAACAGAGGATTTTCTCCAATTGTTCTAATTTACAGAGACCTTCTCCAATTGCCTTCTTTGCTTCTCCAGATATAGAGCTCTTCTTCTTCTTCTCCTCC

Coding sequence (CDS)

ATGGCTTCCAAAGGAGGAATAGCAAGAACAAAGATGAAGATGAGCAAGAAGATCAAGAAGAAGAGCCCACAGAGGATGAAGAAGAGAAATCCTCCTCCTCCTTCTCCTCCTCCAAAGAATCAGGAAACCGAAACCGAAACCGCAGTCGAATTAGCTTCAAGCTCAGCAGCCATGGCGGAAAATGAGAGCATGAGCAGTGATTTGGAAGAGTGCTGCACTCCAAAAGCAGAGAGATTCAGAATCCCAGAGATCAAAATCTGCCCTCCAGCTCCAAAAAAACAGAGGATTTTCTCCAATTGTTCTAATTTACAGAGACCTTCTCCAATTGCCTTCTTTGCTTCTCCAGATATAGAGCTCTTCTTCTTCTTCTCCTCC

Protein sequence

MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAENESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELFFFFSS
Homology
BLAST of MC06g0978 vs. ExPASy Swiss-Prot
Match: Q3ECS5 (Cyclin-dependent protein kinase inhibitor SMR9 OS=Arabidopsis thaliana OX=3702 GN=SMR9 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 5.5e-09
Identity = 50/124 (40.32%), Postives = 67/124 (54.03%), Query Frame = 0

Query: 1   MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE 60
           MASKG          K +++ + +R K+ +   PSPP       T T+    S+S     
Sbjct: 1   MASKG---------KKPLRRTTTRRRKRSHFKNPSPPCSINSDVTSTS--STSTSPTSTA 60

Query: 61  NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF 120
             S  S    CCTP  E+ RIPE+  CPPAPKKQ++  NC+  +R   IAFFA PD+ELF
Sbjct: 61  TPSPVSAESGCCTP--EKSRIPEMLTCPPAPKKQKVAQNCALRRR--QIAFFAPPDVELF 109

Query: 121 FFFS 125
           F F+
Sbjct: 121 FVFA 109

BLAST of MC06g0978 vs. ExPASy Swiss-Prot
Match: F4IWB3 (Cyclin-dependent protein kinase inhibitor SMR13 OS=Arabidopsis thaliana OX=3702 GN=SMR13 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.0e-07
Identity = 43/107 (40.19%), Postives = 57/107 (53.27%), Query Frame = 0

Query: 15  SKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAENESMSSDLEECCTP 74
           SKK +K +    K          P++    T T   + S S A     ++ +    CCTP
Sbjct: 3   SKKARKPNRAEKKLTRSCFKKQVPQHNNINTNTIYSVPSPSPA-----AVLTSPGGCCTP 62

Query: 75  KAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELFF 122
           KA++ RIPE+  CPPAPKKQR+  NC  + R   I FFA P+IELFF
Sbjct: 63  KAKKSRIPEMLTCPPAPKKQRVSKNC--VLRRRQIVFFAPPEIELFF 102

BLAST of MC06g0978 vs. NCBI nr
Match: XP_022154348.1 (cyclin-dependent protein kinase inhibitor SMR9-like [Momordica charantia])

HSP 1 Score: 233 bits (595), Expect = 5.18e-77
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE 60
           MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE
Sbjct: 1   MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE 60

Query: 61  NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF 120
           NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF
Sbjct: 61  NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF 120

Query: 121 FFFSS 125
           FFFSS
Sbjct: 121 FFFSS 125

BLAST of MC06g0978 vs. NCBI nr
Match: KAG6590203.1 (Cyclin-dependent protein kinase inhibitor SMR13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023854.1 Cyclin-dependent protein kinase inhibitor SMR13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 115 bits (288), Expect = 2.77e-30
Identity = 76/137 (55.47%), Postives = 93/137 (67.88%), Query Frame = 0

Query: 1   MASKGG--------IARTKMKMSKKIKKKSPQRMK----KRNPPPPSPPPKNQETETETA 60
           MASKGG         A+T++K ++KIKK   Q  +    KR           +E E  ++
Sbjct: 1   MASKGGGRRNKGKKTAKTQLKKNQKIKKMKDQSKRIVSTKRT----------EEIEVSSS 60

Query: 61  VELASSSAAMAENESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSP 120
           + +A+  AAM E+ S SSD EEC TPKAERFRIPEIKICPPAPKKQRI SNCS+ Q+PSP
Sbjct: 61  IAMAAM-AAMEESASSSSDFEECSTPKAERFRIPEIKICPPAPKKQRITSNCSS-QKPSP 120

Query: 121 IAFFASPDIELFFFFSS 125
           I+FFASPDIE+FFF SS
Sbjct: 121 ISFFASPDIEMFFFLSS 125

BLAST of MC06g0978 vs. NCBI nr
Match: KAG7022087.1 (Cyclin-dependent protein kinase inhibitor SMR13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 104 bits (260), Expect = 5.62e-26
Identity = 55/81 (67.90%), Postives = 64/81 (79.01%), Query Frame = 0

Query: 44  ETETAVELASSSAAMAENESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNL 103
           E E A   +SS+ A  + ++ SSD E C TPKA+RF+IPEI+ICPPAPKKQR  SNCS L
Sbjct: 49  EIEEAAASSSSAMAAIDEQASSSDFEHCSTPKADRFKIPEIQICPPAPKKQRPISNCS-L 108

Query: 104 QRPSPIAFFASPDIELFFFFS 124
           Q+ SPIAFFASPDIELFFFFS
Sbjct: 109 QKHSPIAFFASPDIELFFFFS 128

BLAST of MC06g0978 vs. NCBI nr
Match: XP_023530358.1 (cyclin-dependent protein kinase inhibitor SMR9-like [Cucurbita pepo subsp. pepo] >KAG6588189.1 Cyclin-dependent protein kinase inhibitor SMR13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 104 bits (260), Expect = 5.78e-26
Identity = 55/81 (67.90%), Postives = 64/81 (79.01%), Query Frame = 0

Query: 44  ETETAVELASSSAAMAENESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNL 103
           E E A   +SS+ A  + ++ SSD E C TPKA+RF+IPEI+ICPPAPKKQR  SNCS L
Sbjct: 50  EIEEAAASSSSAMAAIDEQASSSDFEHCSTPKADRFKIPEIQICPPAPKKQRPISNCS-L 109

Query: 104 QRPSPIAFFASPDIELFFFFS 124
           Q+ SPIAFFASPDIELFFFFS
Sbjct: 110 QKHSPIAFFASPDIELFFFFS 129

BLAST of MC06g0978 vs. NCBI nr
Match: KGN66487.1 (hypothetical protein Csa_006949 [Cucumis sativus])

HSP 1 Score: 100 bits (250), Expect = 1.94e-24
Identity = 51/61 (83.61%), Postives = 56/61 (91.80%), Query Frame = 0

Query: 69  EECC-TPKAERFRIPEIKICPPAPKKQRIFSNCSN---LQRPSPIAFFASPDIELFFFFS 125
           EECC TPKAERF+IPEIKICPPAPKK+R+FSN ++   LQRPSPIAFFASPDIELFFFFS
Sbjct: 72  EECCSTPKAERFKIPEIKICPPAPKKRRLFSNSNSNCSLQRPSPIAFFASPDIELFFFFS 131

BLAST of MC06g0978 vs. ExPASy TrEMBL
Match: A0A6J1DK32 (cyclin-dependent protein kinase inhibitor SMR9-like OS=Momordica charantia OX=3673 GN=LOC111021634 PE=4 SV=1)

HSP 1 Score: 233 bits (595), Expect = 2.51e-77
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE 60
           MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE
Sbjct: 1   MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE 60

Query: 61  NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF 120
           NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF
Sbjct: 61  NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF 120

Query: 121 FFFSS 125
           FFFSS
Sbjct: 121 FFFSS 125

BLAST of MC06g0978 vs. ExPASy TrEMBL
Match: A0A0A0M041 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G613590 PE=4 SV=1)

HSP 1 Score: 100 bits (250), Expect = 9.38e-25
Identity = 51/61 (83.61%), Postives = 56/61 (91.80%), Query Frame = 0

Query: 69  EECC-TPKAERFRIPEIKICPPAPKKQRIFSNCSN---LQRPSPIAFFASPDIELFFFFS 125
           EECC TPKAERF+IPEIKICPPAPKK+R+FSN ++   LQRPSPIAFFASPDIELFFFFS
Sbjct: 72  EECCSTPKAERFKIPEIKICPPAPKKRRLFSNSNSNCSLQRPSPIAFFASPDIELFFFFS 131

BLAST of MC06g0978 vs. ExPASy TrEMBL
Match: A0A7J7DRJ7 (Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW04G00704 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 5.71e-21
Identity = 43/54 (79.63%), Postives = 48/54 (88.89%), Query Frame = 0

Query: 71  CCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELFFFFS 124
           C TPKAERFRIPEI  CPPAPKKQR+ +NCS+LQR +PIAFFA PDIELFFFF+
Sbjct: 50  CSTPKAERFRIPEIDTCPPAPKKQRVMTNCSSLQR-TPIAFFAPPDIELFFFFA 102

BLAST of MC06g0978 vs. ExPASy TrEMBL
Match: A0A660KN86 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_009775 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.19e-20
Identity = 44/54 (81.48%), Postives = 48/54 (88.89%), Query Frame = 0

Query: 71  CCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELFFFFS 124
           CCTPKA+RFRIPEI  CPPAPKKQR+ SNCS LQR SPIAFFA PD+ELFFFF+
Sbjct: 63  CCTPKAQRFRIPEISTCPPAPKKQRLVSNCS-LQR-SPIAFFAPPDLELFFFFA 114

BLAST of MC06g0978 vs. ExPASy TrEMBL
Match: A0A6A1UIU7 (Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR0G007916 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 7.71e-20
Identity = 54/95 (56.84%), Postives = 65/95 (68.42%), Query Frame = 0

Query: 39  KNQETETET-AVELASSSAAMAEN--------ESMSSDLEECCTPKAERFRIPEIKICPP 98
           KNQET   + +   A SS  +++N        E++ S    C TPKA+RFRIPEI  CPP
Sbjct: 29  KNQETSRSSPSKSSAKSSNELSKNGEIDFEDVEAIPSSSSGCSTPKAQRFRIPEISTCPP 88

Query: 99  APKKQRIFSNCSNLQRPSPIAFFASPDIELFFFFS 124
           APKKQR+ SNCS LQR SPIAFFA PD+ELFFFF+
Sbjct: 89  APKKQRVISNCS-LQR-SPIAFFAPPDLELFFFFA 121

BLAST of MC06g0978 vs. TAIR 10
Match: AT1G51355.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G20898.1); Has 52 Blast hits to 52 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 50; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 62.0 bits (149), Expect = 3.9e-10
Identity = 50/124 (40.32%), Postives = 67/124 (54.03%), Query Frame = 0

Query: 1   MASKGGIARTKMKMSKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAE 60
           MASKG          K +++ + +R K+ +   PSPP       T T+    S+S     
Sbjct: 1   MASKG---------KKPLRRTTTRRRKRSHFKNPSPPCSINSDVTSTS--STSTSPTSTA 60

Query: 61  NESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELF 120
             S  S    CCTP  E+ RIPE+  CPPAPKKQ++  NC+  +R   IAFFA PD+ELF
Sbjct: 61  TPSPVSAESGCCTP--EKSRIPEMLTCPPAPKKQKVAQNCALRRR--QIAFFAPPDVELF 109

Query: 121 FFFS 125
           F F+
Sbjct: 121 FVFA 109

BLAST of MC06g0978 vs. TAIR 10
Match: AT3G20898.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G51355.1); Has 66 Blast hits to 66 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 66; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 57.8 bits (138), Expect = 7.4e-09
Identity = 43/107 (40.19%), Postives = 57/107 (53.27%), Query Frame = 0

Query: 15  SKKIKKKSPQRMKKRNPPPPSPPPKNQETETETAVELASSSAAMAENESMSSDLEECCTP 74
           SKK +K +    K          P++    T T   + S S A     ++ +    CCTP
Sbjct: 3   SKKARKPNRAEKKLTRSCFKKQVPQHNNINTNTIYSVPSPSPA-----AVLTSPGGCCTP 62

Query: 75  KAERFRIPEIKICPPAPKKQRIFSNCSNLQRPSPIAFFASPDIELFF 122
           KA++ RIPE+  CPPAPKKQR+  NC  + R   I FFA P+IELFF
Sbjct: 63  KAKKSRIPEMLTCPPAPKKQRVSKNC--VLRRRQIVFFAPPEIELFF 102

BLAST of MC06g0978 vs. TAIR 10
Match: AT1G60783.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G10690.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 42.0 bits (97), Expect = 4.2e-04
Identity = 27/79 (34.18%), Postives = 41/79 (51.90%), Query Frame = 0

Query: 45  TETAVELASSSAAMAENESMSSDLEECCTPKAERFRIPEIKICPPAPKKQRIFSNCSNLQ 104
           T   + L+SS+ +  E+E +        TP A+  RIP +  CPPAPKK+++    S   
Sbjct: 34  TVKPISLSSSAVSNTEDEDLCP-----TTPTADSVRIPTVIPCPPAPKKRKLTLKVSY-- 93

Query: 105 RPSPIAFFASPDIELFFFF 124
              P  FF+ PD+E  F +
Sbjct: 94  --GPREFFSPPDLETVFIY 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3ECS55.5e-0940.32Cyclin-dependent protein kinase inhibitor SMR9 OS=Arabidopsis thaliana OX=3702 G... [more]
F4IWB31.0e-0740.19Cyclin-dependent protein kinase inhibitor SMR13 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_022154348.15.18e-77100.00cyclin-dependent protein kinase inhibitor SMR9-like [Momordica charantia][more]
KAG6590203.12.77e-3055.47Cyclin-dependent protein kinase inhibitor SMR13, partial [Cucurbita argyrosperma... [more]
KAG7022087.15.62e-2667.90Cyclin-dependent protein kinase inhibitor SMR13, partial [Cucurbita argyrosperma... [more]
XP_023530358.15.78e-2667.90cyclin-dependent protein kinase inhibitor SMR9-like [Cucurbita pepo subsp. pepo]... [more]
KGN66487.11.94e-2483.61hypothetical protein Csa_006949 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1DK322.51e-77100.00cyclin-dependent protein kinase inhibitor SMR9-like OS=Momordica charantia OX=36... [more]
A0A0A0M0419.38e-2583.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G613590 PE=4 SV=1[more]
A0A7J7DRJ75.71e-2179.63Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW04G00704 ... [more]
A0A660KN863.19e-2081.48Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_009775 PE=4 SV=1[more]
A0A6A1UIU77.71e-2056.84Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR0G007916 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G51355.13.9e-1040.32unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G20898.17.4e-0940.19unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G60783.14.2e-0434.18unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..68
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..70
NoneNo IPR availablePANTHERPTHR33142:SF8CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13coord: 9..124
IPR040389Cyclin-dependent protein kinase inhibitor SMRPANTHERPTHR33142CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13coord: 9..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0978.1MC06g0978.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045839 negative regulation of mitotic nuclear division
biological_process GO:0032875 regulation of DNA endoreduplication
cellular_component GO:0005634 nucleus