MC06g0921 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0921
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	L-ascorbate oxidase
Location	MC06: 7756615 .. 7761900 (-)
RNA-Seq Expression	MC06g0921
Synteny	MC06g0921

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGATTCGAACTTTCAACTTTTTGGCTAAAAGGAGGGATCGAAACAGTTACTAATATATGCAACCTGAACACTATTTAGTTGGTCAAATATTCAAGGCTCCTTTGATAACGTTTATATTTCTAGTTTTTCGTACAAAATCAAGTTTATTGTTTTTTTACTCCCGTTTCAAATTTGTTCCAAAACATAAATAAAATTACTGTTTTATCATTTTTTAATCTATGGGTTGGGGTATGCAATTGATTCTATCTTTCGAGAAAAAACAGAATCAAGAAACGTGAATGTTATCAAGTGAGTCCTGATTAGTATCTCGTAACACATGAGATCGTAAGTTTGAATCTCTTTTACCTGCGGTCGAAATTGACCGTTAAAAGGAAAAAAGAAAAAGTAAAATTCACCCCACCTAACACAGAGGACTGCCTCTAGAATCTAAATTTTCCATTATAAAAAGGAGCCCAACACTAGAGCCAATGTGCCATCCCTCGCCCATCAACTTTTTTCTCTGTTCTTCCTTTTTACTCATTGTTTACAATTCTTTCTCCCATTTCCCTCTTTTTTTTTTAGATGGGAATATTTGCAGAGCCATTTCTTGGACCCTTTCTGGTCATCTTCTTTGTTTTGATCTCTGCGTTTGGAGCTTTGTCTGAGGGCTCCAAAATCAGACACCATCGATGGGAGGTTGAGTACATGTTTTGGTCCCCGGACTGCGTTGAAAACGTCGTTATGGGAATCAACCACCAGTTCCCCGGCCCGACGATCAGAGCCAATGCAGGCGACACCGTCGTTGTCGAGCTTACCAACAAGCTCCACACCGAGGGCGTCGTCATTCACTGGCATGGGATACTTCAGCACGGAACTCCGTGGGCTGATGGCACTGCTTCCATCTCGCAATGCGCCATCAACCCCGGCGAAACCTTCGTCTACCGATTCGTAGTTGACAAGGTGAGATTGGGGCCGTACTTTCTTTTTTCTTGTTTCTTTTTCTCTCGTCAGAAGGATAAGTTTCGACTCTCGTGCAGGCGGGAACGTTCTTCTATCATGGCCATTTAGGAATGCAAAGATCTGCAGGACTATATGGATCTCTAATAGTAGATCCAGCAGAAGGAGAGAAAGAGCCATTCCATTATGATGAAGAGATTAACTTATTGCTTAGTGACTGGTGGCATCAGAGCGTTCATAGACAAGAGGTCGGCCTCAGTTCCATCCCGTTTCGTTGGATCGGCGAACCCCAGGTAAAAGATAAAATATCAACCGACATGTCGAAATCGAACAAATCCGATGCATTTTTATCTGCAACATGGGTGCATATTCTATATTGATATTTCTATATTTCATACAAATATATTTTTTTTCCTTTCATAATTTTTTATCCGCAACATGCTTGCATATCGTATATTGATATTTCAGTAAAGGTGAGATCTCGATATTTTTTTCTATTAATATCAATTATTTTAAATCATTAATCTGCACTGCTGAACTCATCACTGTTTGAAATGATATTTCTTTTTCCTTCTTTCCACATGAAAGAAAAGTTAGATTTTTCAATGTTCAAAAGTGTATTGCTTTACACAAGTGTACACAAATATTCTCTCTTCTCTCTCTCTGGGTTTCTTCCTTGAATTATGGTGCCTTTTTTTAACTTTTGCTTGACATTTCAATGTGACAATATCATTTGCTTCTAATTATTATTATTATTATTTAAACATGAGTATCTAAGGATGGTTAAAATTTTAGAATTTAACCGTTTGGAAGACAATATCTTAGGAGAAATTTATAACATTTAAACATAATTTAGTGAAAGATATTAATTAATTTTTTTTAAGTCGAACACCTAATCACATCCAATCAATCTACATATATTCTGTCAATTTTTTTTAGTTCAATATCTCTGTGGAATAGAGATTTGAACCTTAGATTTTTTTAATCAAGGATAAATGTTTTAATTATTTAGATTTACAGTATCTTGTCATATATTTATATTAAGAGAAAGTATATGTTTTATTTGATCACGGCGGACAGAATAAAACATATTGAAAAATACCTACCAGCCTCTCTAAATTTCTTCCTTGCTTGAAATATGGTTTTTCTTATTTATTATTGTGTAAGTTTTTTATTTTTATTTTTATTATTTTTGTGATGTAAAAGTTCAAGGTGAGAATGTGGTGGGTAGGACCATATGCAAAAGATGCTCTCTTTTCAAAATTATATATATATATATATATATATTTTTTTTTTTTTTGAGAAAAATATATTTTTTATTATTATAAAGTACATGTCCAAACAAAAGCTGTCATGATGTGTTTGGACAAAATATTATAGTATTCAATAAATCATATATATCTATCTTCAACCCCACGTTTCTTTATCCATTGTATTTCACGATGAAATTTAGCTGTACTCTACTTTATTATTATATTATTATTATTTTAATTTTGAGTTAAAACTTTTTGTTAAAAAATATACGGTTCAAATTGCTTTATTAATTATTATTACTTTTTACTTAAACTAATCTTTAACCAAGTGGGAACTAGTCAAGTTTTGTTCCTTTTTAATTTATTTGAAAAGTTCAAAATGTTTCGATAACTTTATCATTATTTTGATGCCAAAGCGAATATAGTTCAGTGATATATATCTTCAACAAAGGGGTGTGAATTCGATCCCCCACACCCGCATGTTAAACTAAAAAAAATCATTATTTTGATAAAATCTATAAGTTAAATACATATAATTATGAGTTGAATAAACTTAAACTTTGATTTCTTCTCACATATACGACTTTAGCATTTACTTTAAAAGACCTATTATAGATGGCTTAAATTTACCATTAATGTCTAGTTCATCAACCTAAAGTGTGTAACAGCACATACGAGAGTAGATTTGGACCCACGACCTTATGGTCGGGATACATGTCTTAATCAGTTGTGAGCAGGTTGAAAATAACGGAGTCGATTTTGGAAGTTTAATTATTTATTTATTATATTTTGTTTTACAGAGCTTGCTGATAAATGGGAGAGGTCAATTCAATTGTTCAATTGCAGCCAAATATAACAACACATTGCAGCAATGTGCGTTGAAAGGGAGTGAACAATGTGCACCATTTATCCTCCGTGTCCTTCCCAATAAGATCTACCGTATTAGAATTGCCAGCACCACAGCATTGGCTTCTCTCAACTTCGCCATTGGGGTACTTTTTTTCTTTCTTTCTTTTTTTTTACCACTTTATTGCTCTTTAAATCATGACAAAAAAAAAAAAAGGAAAAAGAAATTCAACCTAAGAAATTATTAATTAGTAATGATGTCTTGAAGACTATTTTAATAACTTTTCCGTCGGACTCTGAAAGCTCAAAAAAGTGGGATAGTTAAAGATTTGAAGTGAAAGATGGGATGATGTCGTTTAGATAAGTTATGGTGAATATAATATCCCACCATCTTTTAAAGTGGGAAAGGTACAATCCATTTGATATATACTCGTGCCATTAAAGGAAAAAACACCAAAAATAAAGGGGATGGGGCAAAAATCTATCAAAGAAAAAACCTTGTCTTGCACTATTTTTTAGTAAATTGACAACTTTATACCACTCCATATTTTGTACTTGAACATATATATTTATAGTGGTAAAATGACGACATTTATCTAGCTCTAAAATTAATTGTTAATCTTTATTTAATAAAATTAAACTACATCTAAAAGTAGAAATTGTTCCTTAATTATAAATTAAACTGCATTTTCTTTTACGGAATTAAACTGCATTTAAAAGTAGAAGTTTCCTCTCTGACATTTAATTCACCGCATGATATTTAATTTGAAACCACTCCATTCAATTTGAAATAAATAATTATAATTTTTTAAAATTATATGGACTAAACTAAAATTTTAAAATCATAACTTTGTAATTTAATTTTTTTTTACTAAAGGAGTATAGTTAAGCAGTACTTTGACGTTCCAAAACTTTCTTATTTTTTTTCTTTCCGATTATTATTTAAATATACATCAATTTTCTCACGTGCTTAATTTTTTTCCTTCAGAATCACAAACTGTTGGTGGTGGAAGCCGACGGAAACTACGTTCAACCATTTCTGACCAACGACATAGACATTTACTCCGGCGAGGCCTACTCCGTCCTCATAAACACCGACCAAAACCCATCGGAAAATTACTGGGTCTCCGTCGGCGTCCGCGGCCGCCAGCCAAACACGCCGCCGGGCCTCACCCTCCTCAACTACGGCCCTAACTCCATCTCCAAATTACCCTCCTCTGCGCCGCCGCCCACCCCCCGGTGGGACGATTTCGCCCGGAGCAAGAATTTCACCACCCGAATCACCGCCGCCATGGGATCCCCGAGGCCGCCGGCGACGTTCCACCGCCGCATCGTCCTCCTCAACACCCAGAACCGAATCAACGGCGCAGTCAAGTGGGCCATCAACAACGTCTCCCTGACTCTCCCGCCGACGCCGTACCTCGGCTCCATAAAATTCCGGCTAAACGCCGCCTTCGACCAGAAACCCCCGCCGGAAACATTCCCACAGGACTACGACATCCACCGCCCGCCGCCGAACCCGAACACCACCACCAGCAGCGGCGTTTACCGGCTAGATCTGGGGTCGACGGTGGACGTGATCCTCCAAAACTCGAACGCGCTGAACGAAAAGGTGAGCGAGATCCACCCGTGGCATCTGCACGGCCACGATTTCTGGGTGTTGGGGTACGGAGAGGGGAAATTCTCCGGCGGCGCCGATGAGAAGAAATTGAATCTGAAGAACCCGCCGCTGCGGAACACGGCGGTGATTTTCCCGTACGGGTGGACGGCGCTGAGATTCGTGGCGGACAATCCCGGAGTTTGGGCGTTCCATTGCCACATCGAGCCGCATCTCCATATGGGGATGGGGGTGGTGTTCGCCGAAGGGGTTCATAAGGTCGGCAACGTTCCGAAGCAGGTTCTGACTTGTGGCCTCACTGGAAAGACGCTGATGAAGGACGAACCCTAAACGAACCCCTGAAAAACCCTAAACAAACCCCTGAAAAACCCTTATGAACCCCTAAAAAACCAGGGACTTGGTTAGCTAGCAGTGCGATGCAGATCGGTGGGAATTAAAATATTTTTTTAATATAAAATGTGAGTAGAGATTGACCTTTTAGGTCATACATTAGTGTCTTGACTAGTTAATTGAGTTATGTTCTAAGGCCTAAGGGGCCGTTATAATTGTATCGTTTATGTGTAATTTTCTTCTCATATGTTTGAGTTAAATGTTAAAATGATGAAATTTGTAATGGATAGTTAAGGATCCA

mRNA sequence

GAAGATTCGAACTTTCAACTTTTTGGCTAAAAGGAGGGATCGAAACAGTTACTAATATATGCAACCTGAACACTATTTAGTTGGTCAAATATTCAAGGCTCCTTTGATAACGTTTATATTTCTAGTTTTTCGTACAAAATCAAGTTTATTGTTTTTTTACTCCCGTTTCAAATTTGTTCCAAAACATAAATAAAATTACTGTTTTATCATTTTTTAATCTATGGGTTGGGGTATGCAATTGATTCTATCTTTCGAGAAAAAACAGAATCAAGAAACGTGAATGTTATCAAGTGAGTCCTGATTAGTATCTCGTAACACATGAGATCGTAAGTTTGAATCTCTTTTACCTGCGGTCGAAATTGACCGTTAAAAGGAAAAAAGAAAAAGTAAAATTCACCCCACCTAACACAGAGGACTGCCTCTAGAATCTAAATTTTCCATTATAAAAAGGAGCCCAACACTAGAGCCAATGTGCCATCCCTCGCCCATCAACTTTTTTCTCTGTTCTTCCTTTTTACTCATTGTTTACAATTCTTTCTCCCATTTCCCTCTTTTTTTTTTAGATGGGAATATTTGCAGAGCCATTTCTTGGACCCTTTCTGGTCATCTTCTTTGTTTTGATCTCTGCGTTTGGAGCTTTGTCTGAGGGCTCCAAAATCAGACACCATCGATGGGAGGTTGAGTACATGTTTTGGTCCCCGGACTGCGTTGAAAACGTCGTTATGGGAATCAACCACCAGTTCCCCGGCCCGACGATCAGAGCCAATGCAGGCGACACCGTCGTTGTCGAGCTTACCAACAAGCTCCACACCGAGGGCGTCGTCATTCACTGGCATGGGATACTTCAGCACGGAACTCCGTGGGCTGATGGCACTGCTTCCATCTCGCAATGCGCCATCAACCCCGGCGAAACCTTCGTCTACCGATTCGTAGTTGACAAGGCGGGAACGTTCTTCTATCATGGCCATTTAGGAATGCAAAGATCTGCAGGACTATATGGATCTCTAATAGTAGATCCAGCAGAAGGAGAGAAAGAGCCATTCCATTATGATGAAGAGATTAACTTATTGCTTAGTGACTGGTGGCATCAGAGCGTTCATAGACAAGAGGTCGGCCTCAGTTCCATCCCGTTTCGTTGGATCGGCGAACCCCAGAGCTTGCTGATAAATGGGAGAGGTCAATTCAATTGTTCAATTGCAGCCAAATATAACAACACATTGCAGCAATGTGCGTTGAAAGGGAGTGAACAATGTGCACCATTTATCCTCCGTGTCCTTCCCAATAAGATCTACCGTATTAGAATTGCCAGCACCACAGCATTGGCTTCTCTCAACTTCGCCATTGGGAATCACAAACTGTTGGTGGTGGAAGCCGACGGAAACTACGTTCAACCATTTCTGACCAACGACATAGACATTTACTCCGGCGAGGCCTACTCCGTCCTCATAAACACCGACCAAAACCCATCGGAAAATTACTGGGTCTCCGTCGGCGTCCGCGGCCGCCAGCCAAACACGCCGCCGGGCCTCACCCTCCTCAACTACGGCCCTAACTCCATCTCCAAATTACCCTCCTCTGCGCCGCCGCCCACCCCCCGGTGGGACGATTTCGCCCGGAGCAAGAATTTCACCACCCGAATCACCGCCGCCATGGGATCCCCGAGGCCGCCGGCGACGTTCCACCGCCGCATCGTCCTCCTCAACACCCAGAACCGAATCAACGGCGCAGTCAAGTGGGCCATCAACAACGTCTCCCTGACTCTCCCGCCGACGCCGTACCTCGGCTCCATAAAATTCCGGCTAAACGCCGCCTTCGACCAGAAACCCCCGCCGGAAACATTCCCACAGGACTACGACATCCACCGCCCGCCGCCGAACCCGAACACCACCACCAGCAGCGGCGTTTACCGGCTAGATCTGGGGTCGACGGTGGACGTGATCCTCCAAAACTCGAACGCGCTGAACGAAAAGGTGAGCGAGATCCACCCGTGGCATCTGCACGGCCACGATTTCTGGGTGTTGGGGTACGGAGAGGGGAAATTCTCCGGCGGCGCCGATGAGAAGAAATTGAATCTGAAGAACCCGCCGCTGCGGAACACGGCGGTGATTTTCCCGTACGGGTGGACGGCGCTGAGATTCGTGGCGGACAATCCCGGAGTTTGGGCGTTCCATTGCCACATCGAGCCGCATCTCCATATGGGGATGGGGGTGGTGTTCGCCGAAGGGGTTCATAAGGTCGGCAACGTTCCGAAGCAGGTTCTGACTTGTGGCCTCACTGGAAAGACGCTGATGAAGGACGAACCCTAAACGAACCCCTGAAAAACCCTAAACAAACCCCTGAAAAACCCTTATGAACCCCTAAAAAACCAGGGACTTGGTTAGCTAGCAGTGCGATGCAGATCGGTGGGAATTAAAATATTTTTTTAATATAAAATGTGAGTAGAGATTGACCTTTTAGGTCATACATTAGTGTCTTGACTAGTTAATTGAGTTATGTTCTAAGGCCTAAGGGGCCGTTATAATTGTATCGTTTATGTGTAATTTTCTTCTCATATGTTTGAGTTAAATGTTAAAATGATGAAATTTGTAATGGATAGTTAAGGATCCA

Coding sequence (CDS)

ATGGGAATATTTGCAGAGCCATTTCTTGGACCCTTTCTGGTCATCTTCTTTGTTTTGATCTCTGCGTTTGGAGCTTTGTCTGAGGGCTCCAAAATCAGACACCATCGATGGGAGGTTGAGTACATGTTTTGGTCCCCGGACTGCGTTGAAAACGTCGTTATGGGAATCAACCACCAGTTCCCCGGCCCGACGATCAGAGCCAATGCAGGCGACACCGTCGTTGTCGAGCTTACCAACAAGCTCCACACCGAGGGCGTCGTCATTCACTGGCATGGGATACTTCAGCACGGAACTCCGTGGGCTGATGGCACTGCTTCCATCTCGCAATGCGCCATCAACCCCGGCGAAACCTTCGTCTACCGATTCGTAGTTGACAAGGCGGGAACGTTCTTCTATCATGGCCATTTAGGAATGCAAAGATCTGCAGGACTATATGGATCTCTAATAGTAGATCCAGCAGAAGGAGAGAAAGAGCCATTCCATTATGATGAAGAGATTAACTTATTGCTTAGTGACTGGTGGCATCAGAGCGTTCATAGACAAGAGGTCGGCCTCAGTTCCATCCCGTTTCGTTGGATCGGCGAACCCCAGAGCTTGCTGATAAATGGGAGAGGTCAATTCAATTGTTCAATTGCAGCCAAATATAACAACACATTGCAGCAATGTGCGTTGAAAGGGAGTGAACAATGTGCACCATTTATCCTCCGTGTCCTTCCCAATAAGATCTACCGTATTAGAATTGCCAGCACCACAGCATTGGCTTCTCTCAACTTCGCCATTGGGAATCACAAACTGTTGGTGGTGGAAGCCGACGGAAACTACGTTCAACCATTTCTGACCAACGACATAGACATTTACTCCGGCGAGGCCTACTCCGTCCTCATAAACACCGACCAAAACCCATCGGAAAATTACTGGGTCTCCGTCGGCGTCCGCGGCCGCCAGCCAAACACGCCGCCGGGCCTCACCCTCCTCAACTACGGCCCTAACTCCATCTCCAAATTACCCTCCTCTGCGCCGCCGCCCACCCCCCGGTGGGACGATTTCGCCCGGAGCAAGAATTTCACCACCCGAATCACCGCCGCCATGGGATCCCCGAGGCCGCCGGCGACGTTCCACCGCCGCATCGTCCTCCTCAACACCCAGAACCGAATCAACGGCGCAGTCAAGTGGGCCATCAACAACGTCTCCCTGACTCTCCCGCCGACGCCGTACCTCGGCTCCATAAAATTCCGGCTAAACGCCGCCTTCGACCAGAAACCCCCGCCGGAAACATTCCCACAGGACTACGACATCCACCGCCCGCCGCCGAACCCGAACACCACCACCAGCAGCGGCGTTTACCGGCTAGATCTGGGGTCGACGGTGGACGTGATCCTCCAAAACTCGAACGCGCTGAACGAAAAGGTGAGCGAGATCCACCCGTGGCATCTGCACGGCCACGATTTCTGGGTGTTGGGGTACGGAGAGGGGAAATTCTCCGGCGGCGCCGATGAGAAGAAATTGAATCTGAAGAACCCGCCGCTGCGGAACACGGCGGTGATTTTCCCGTACGGGTGGACGGCGCTGAGATTCGTGGCGGACAATCCCGGAGTTTGGGCGTTCCATTGCCACATCGAGCCGCATCTCCATATGGGGATGGGGGTGGTGTTCGCCGAAGGGGTTCATAAGGTCGGCAACGTTCCGAAGCAGGTTCTGACTTGTGGCCTCACTGGAAAGACGCTGATGAAGGACGAACCCTAA

Protein sequence

MGIFAEPFLGPFLVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPNKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP

Homology

[Close]

BLAST of MC06g0921 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 1.9e-282
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0


Query: 4   FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
           F +PFL  FLV+   +I  FG  LSE    KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10  FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69

Query: 64  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70  PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129

Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189

Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
           QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN  L+QC L G E+CAPFIL V P 
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249

Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
           K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309

Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
           P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369

Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q 
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQN 429

Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
           PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN  +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489

Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
           HDFWVLGYGEGKF    DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549

Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 581
           PHLHMGMGVVFAEGVH VG +P + L CG T   L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGST--ALVKNYP 584

[Close]

BLAST of MC06g0921 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 959.1 bits (2478), Expect = 2.2e-278
Identity = 448/579 (77.37%), Postives = 494/579 (85.32%), Query Frame = 0


Query: 1   MGIFAEPFLGPFLVIFFV-LISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQ 60
           MG   EP    FL++FF  LI AFG  SEGS+IRH++WEVEYMFW+PDC EN+VMGIN Q
Sbjct: 4   MGKAREP---NFLILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQ 63

Query: 61  FPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFV 120
           FPGPTIRANAGDTVVVEL NKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF 
Sbjct: 64  FPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFF 123

Query: 121 YRFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVH 180
           Y F VD  GTFFYHGHLGMQRSAGLYGSLIVDP +G+KEPFHYD EINLLLSDWWHQS+H
Sbjct: 124 YNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIH 183

Query: 181 RQEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLP 240
           +QEVGLSS P RWIGEPQ++L+NGRGQF+CSIAAKY++ L+ C LKGSE CAP+I  V+P
Sbjct: 184 KQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMP 243

Query: 241 NKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQ 300
            K YRIRIASTTALA+LNFAIGNH LLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQ
Sbjct: 244 KKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQ 303

Query: 301 NPSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRI 360
           NPSENYWVSVG RGR PNTPPGLTLLNY PNS+SKLP+S PP TP WDDF RSKNFT RI
Sbjct: 304 NPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRI 363

Query: 361 TAAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQ 420
           TAAMGSP+PP   +RRI LLNTQN ING VKWAIN+VSL LPPTPYLG++KF L  AFDQ
Sbjct: 364 TAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQ 423

Query: 421 KPPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLH 480
            PPPE FP+DYDI  PP N  T   +GVY+  +G  VDVILQN+N + E +SEIHPWHLH
Sbjct: 424 NPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLH 483

Query: 481 GHDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHI 540
           GHDFWVLGYG+GKF+   +E  LNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHI
Sbjct: 484 GHDFWVLGYGDGKFT-AEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 543

Query: 541 EPHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKD 579
           EPHLHMGMGVVFAEGV KVG +P + L CG T K+L+ +
Sbjct: 544 EPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINN 578

[Close]

BLAST of MC06g0921 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 2.4e-272
Identity = 430/549 (78.32%), Postives = 476/549 (86.70%), Query Frame = 0


Query: 30  SKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTVVVELTNKLHTEGVVIH 89
           S+IRH++WEVEYMFW+P+C EN+VMGIN QFPGPTIRANAGD+VVVELTNKLHTEGVVIH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 90  WHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYHGHLGMQRSAGLYGSLI 149
           WHGILQ GTPWADGTASISQCAINPGETF Y F VD  GTFFYHGHLGMQRSAGLYGSLI
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 150 VDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWIGEPQSLLINGRGQFNC 209
           VDP +G+KEPFHYD EINLLLSDWWHQS+H+QEVGLSS P RWIGEPQ++L+NGRGQF+C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180

Query: 210 SIAAKYNNTLQQCALKGSEQCAPFILRVLPNKIYRIRIASTTALASLNFAIGNHKLLVVE 269
           SIAAKY++ L+ C LKGSE CAP+I  V P K YRIRIASTTALA+LNFAIGNH+LLVVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240

Query: 270 ADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRGRQPNTPPGLTLLNYGP 329
           ADGNYVQPF T+DIDIYSGE+YSVLI TDQNPSENYWVSVG R R PNTPPGLTLLNY P
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300

Query: 330 NSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFHRRIVLLNTQNRINGAV 389
           NS+SKLP+S PP TP WDDF RSKNFT RITAAMGSP+PP  F+RRI LLNTQN ING V
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYV 360

Query: 390 KWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYDIHRPPPNPNTTTSSGVYR 449
           KWAIN+VSL LPPTPYLG++K+ L  AFDQ PPPE FP+DYDI  PP N  T   +GVY+
Sbjct: 361 KWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQ 420

Query: 450 LDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKFSGGADEKKLNLKNPPL 509
             +G  VDVILQN+N + E +SE HPWHLHGHDFWVLGYG+GKFS   +E  LNLKNPPL
Sbjct: 421 FKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFS-AEEESSLNLKNPPL 480

Query: 510 RNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHKVGNVPKQVLTCG 569
           RNT VIFPYGWTA+RFVADNPGVWAFHCHIEPHLHMGMGVVFAEGV KVG +P + L CG
Sbjct: 481 RNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACG 540

Query: 570 LTGKTLMKD 579
            T K+L+ +
Sbjct: 541 GTAKSLINN 548

[Close]

BLAST of MC06g0921 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 1.2e-247
Identity = 401/573 (69.98%), Postives = 465/573 (81.15%), Query Frame = 0


Query: 8   FLGPFLVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRA 67
           FL  FL+   +L  +       +K RH +W+VEY+ WSPD  E+VVMGIN QFPGPTIRA
Sbjct: 6   FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65

Query: 68  NAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKA 127
            AGDTV V LTNKLHTEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETF+YRF VDKA
Sbjct: 66  KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125

Query: 128 GTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSS 187
           GT+FYHGH GMQRSAGLYGSLIV+  EGEKEPFHYD E NLLLSDWWH+  H QEV LSS
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 185

Query: 188 IPFRWIGEPQSLLINGRGQFNCSIAAKYNN-TLQQCALKGSEQCAPFILRVLPNKIYRIR 247
            P RWIGEPQ+LL+NGRGQ+NCS+AA+++   L QC L+G EQ AP ILRV PNKIYR+R
Sbjct: 186 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 245

Query: 248 IASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYW 307
           +ASTTAL SL+ AIG HK++VVEADGNYVQPF   D+DIYSGE+YSVL  TDQ+P++NYW
Sbjct: 246 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 305

Query: 308 VSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSP 367
           +S+ VRGR+P TP GLTLLNY PNS SK P+  PP  P W+D+  SK+F+ +I A MGSP
Sbjct: 306 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSP 365

Query: 368 RPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETF 427
           +PP   HRRI+LLNTQN+I+G  KWAINNVSL LP   YLGSI++ +N AFD KPPP+ F
Sbjct: 366 KPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPDNF 425

Query: 428 PQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVL 487
           P+DYD+ +  PN N+T  +GVY L   +T+D+ILQN+NAL + VSEIHPWHLHGHDFWVL
Sbjct: 426 PKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVL 485

Query: 488 GYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMG 547
           GYGEGKFS   D KK NLKNPPLRNTAVIFP+GWTALRFV DNPGVWAFHCHIEPHLHMG
Sbjct: 486 GYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMG 545

Query: 548 MGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDE 580
           MGV+FAEGVH V  +PK+ L CGLTGK LM ++
Sbjct: 546 MGVIFAEGVHLVKKIPKEALACGLTGKMLMSNK 576

[Close]

BLAST of MC06g0921 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 3.5e-231
Identity = 370/563 (65.72%), Postives = 437/563 (77.62%), Query Frame = 0


Query: 15  IFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTVV 74
           +++++  A  A +  + +R + WEVEY F  PDC E +VM +N QFPGPTI A AGDT+V
Sbjct: 3   MWWIVAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIV 62

Query: 75  VELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYHG 134
           V LTNKL TEG+VIHWHGI Q G+PWADG A ++QCAI+PGETF Y F VDK GT FYHG
Sbjct: 63  VHLTNKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHG 122

Query: 135 HLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWIG 194
           H GMQRSAGLYGSLI+D A+G+KEP  YD E NLLLSDWWH+ V  QE+GLSS P RWIG
Sbjct: 123 HYGMQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIG 182

Query: 195 EPQSLLINGRGQFNCSIAAKYNNT-LQQCALKGSEQCAPFILRVLPNKIYRIRIASTTAL 254
           E QS+LINGRGQFNCS+AA++++T L  C  K  +QCAP  L V PNK YRIR+AS+TAL
Sbjct: 183 EAQSILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTAL 242

Query: 255 ASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRG 314
           ASLNFA+  HKL+VVEADGNY+ PF T+DIDIYSGE YSVL+ TDQ+PS+NY+++ GVRG
Sbjct: 243 ASLNFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRG 302

Query: 315 RQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFH 374
           R+PNTPP LT+LNY     S+LP+S PP TPRW+DF RSKNF+ +I AAMGSP PP TF 
Sbjct: 303 RKPNTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFD 362

Query: 375 RRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYDIH 434
            R++LLNTQN I G  KWAINNVSL +P TPYLGS+K+ L   F++  PP+ +P DYDI 
Sbjct: 363 ERLILLNTQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIM 422

Query: 435 RPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKF 494
            PP N N    +         TVDVILQN+N LN   SEIHPWHLHGHDFWVLGYGEGKF
Sbjct: 423 TPPRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKF 482

Query: 495 SGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAE 554
             G DEK  NLKNPPLRNT  ++PYGWTALRFV DNPGVW FHCHIEPHLHMGMGVVFAE
Sbjct: 483 KPGVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 542

Query: 555 GVHKVGNVPKQVLTCGLTGKTLM 577
           G++++G VP + L CGLT + LM
Sbjct: 543 GLNRIGKVPDEALGCGLTKQFLM 565

[Close]

BLAST of MC06g0921 vs. NCBI nr
Match: XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 986 bits (2549), Expect = 0.0
Identity = 464/584 (79.45%), Postives = 505/584 (86.47%), Query Frame = 0


Query: 2   GIFAEPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGI 61
            +F +PFL     IFF+L   FG    LSE    KIRH++WEVEYMFWSPDCVEN+VMGI
Sbjct: 8   SLFPKPFL-----IFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGI 67

Query: 62  NHQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGE 121
           + QFPGPTIRAN GDTVVVELTNKLHTEGVVIHWHGI+Q GTPWADGTASISQCAINPGE
Sbjct: 68  DGQFPGPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAINPGE 127

Query: 122 TFVYRFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQ 181
           TF YRFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG+ EPFHYDEEINLLLSDWWHQ
Sbjct: 128 TFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQ 187

Query: 182 SVHRQEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILR 241
           SVH+QEVGLSS P RWIGEPQ++LINGRGQF+CSIAAKYNN L+QC LKG EQCAPFIL 
Sbjct: 188 SVHKQEVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPFILH 247

Query: 242 VLPNKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLIN 301
           VLPNK YRIRIASTTALASLNFAIGNH+LLVVEADGNY+QPFLT+DIDIYSGE+YSVLI 
Sbjct: 248 VLPNKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSVLIT 307

Query: 302 TDQNPSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFT 361
           TDQ  SENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT
Sbjct: 308 TDQPSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSKNFT 367

Query: 362 TRITAAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAA 421
            RI AAMGSP+PP  ++RRI LLNTQNR+NG VKWAINNVSL LPPTPYL ++KF+LN A
Sbjct: 368 FRIFAAMGSPKPPMRYNRRIFLLNTQNRMNGFVKWAINNVSLALPPTPYLAAMKFKLNTA 427

Query: 422 FDQKPPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPW 481
           FDQ PPPETFP DYDI+ PP N NT T +GVYR ++G  VDVILQN+N LN+  SEIHPW
Sbjct: 428 FDQNPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSEIHPW 487

Query: 482 HLHGHDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFH 541
           HLHGHDFWVLGYGEGKFS   DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFH
Sbjct: 488 HLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 547

Query: 542 CHIEPHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           CHIEPHLHMGMGVVFAEGVHKVG +P + L CG   K L+ + P
Sbjct: 548 CHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGAAKALINNNP 586

[Close]

BLAST of MC06g0921 vs. NCBI nr
Match: NP_001284441.1 (L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis melo])

HSP 1 Score: 972 bits (2514), Expect = 0.0
Identity = 459/580 (79.14%), Postives = 499/580 (86.03%), Query Frame = 0


Query: 6   EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
           +PF     ++FFVL   FG    LSE    KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7   KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66

Query: 66  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67  PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126

Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186

Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
           QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P 
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246

Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
           KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306

Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
           PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366

Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q 
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426

Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
           PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN   SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486

Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
           HDFWVLGYGEGKFS   D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546

Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           PHLHMGMGV+FAEGVHKVG +P   + CG T   ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYP 584

[Close]

BLAST of MC06g0921 vs. NCBI nr
Match: KAA0055636.1 (L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 458/580 (78.97%), Postives = 498/580 (85.86%), Query Frame = 0


Query: 6   EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
           +PF     ++FFVL   FG    LSE    KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7   KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66

Query: 66  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67  PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126

Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186

Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
           QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P 
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246

Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
           K YRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306

Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
           PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366

Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q 
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426

Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
           PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN   SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486

Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
           HDFWVLGYGEGKFS   D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546

Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           PHLHMGMGV+FAEGVHKVG +P   + CG T   ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYP 584

[Close]

BLAST of MC06g0921 vs. NCBI nr
Match: XP_023517000.1 (L-ascorbate oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 452/568 (79.58%), Postives = 490/568 (86.27%), Query Frame = 0


Query: 11  PFLVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAG 70
           P L+I F+   A        KI+H++WEVEYMFWSPDCVENVVMGIN QFPGPTIRANAG
Sbjct: 8   PLLLILFLFSIALTPSMALPKIKHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANAG 67

Query: 71  DTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTF 130
           DTVVVELTNKLHTEGVVIHWHGILQHG+PWADGTASISQCAINPGETF YRFVVDKAGTF
Sbjct: 68  DTVVVELTNKLHTEGVVIHWHGILQHGSPWADGTASISQCAINPGETFTYRFVVDKAGTF 127

Query: 131 FYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPF 190
           FYHGHLGMQRSAGLYGSLIVDP EG+ EPFHYDEEINLLLSDWWHQSVH+QEVGLSS P 
Sbjct: 128 FYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHKQEVGLSSNPM 187

Query: 191 RWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPNKIYRIRIAST 250
           RWIGEPQ++LINGRGQFNCSIAAKY++  + CALKG EQCAPFI+ V PNK  RIRIAST
Sbjct: 188 RWIGEPQTILINGRGQFNCSIAAKYHSGSKPCALKGGEQCAPFIMHVHPNKTVRIRIAST 247

Query: 251 TALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVG 310
           TALASLNFAIGNH+LLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQNPSENYWVS+G
Sbjct: 248 TALASLNFAIGNHQLLVVEADGNYVQPFRTSDIDIYSGESYSVLITTDQNPSENYWVSIG 307

Query: 311 VRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPA 370
           VR R+PNTPPGLTLLNY PNS +KLP   PP TP W+DF RSKNFT RI + M SP+PP 
Sbjct: 308 VRARRPNTPPGLTLLNYLPNSPAKLPILPPPETPDWEDFDRSKNFTFRIFSTMSSPKPPM 367

Query: 371 TFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDY 430
            F RRI LLNTQNR+NG  KWAINNVSL LPPTPYLG++K+RL  AF+Q PPPE FP DY
Sbjct: 368 RFDRRIFLLNTQNRLNGFTKWAINNVSLALPPTPYLGAMKYRLKTAFNQNPPPENFPSDY 427

Query: 431 DIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGE 490
           +I  PPPNPNTT  +GVYR  LG TVDVILQN+N LN   SEIHPWHLHGHDFWVLGYG+
Sbjct: 428 NIDIPPPNPNTTIGNGVYRFKLGETVDVILQNANMLNPNTSEIHPWHLHGHDFWVLGYGD 487

Query: 491 GKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVV 550
           GKFS     +KLNLKNPPLRNT VIFP GWTA+RFVADNPGVWAFHCHIEPHLHMGMGVV
Sbjct: 488 GKFSAAVHGRKLNLKNPPLRNTVVIFPNGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVV 547

Query: 551 FAEGVHKVGNVPKQVLTCGLTGKTLMKD 578
           FAEGV KVG +PK+ L CG+T K LMK+
Sbjct: 548 FAEGVEKVGRIPKKALACGVTAKALMKN 575

[Close]

BLAST of MC06g0921 vs. NCBI nr
Match: XP_004149015.1 (L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags: Precursor [Cucumis sativus] >AAA33119.1 ascorbate oxidase precursor (EC 1.10.3.3) [Cucumis sativus] >KGN66418.1 hypothetical protein Csa_007350 [Cucumis sativus])

HSP 1 Score: 964 bits (2492), Expect = 0.0
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0


Query: 4   FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
           F +PFL  FLV+   +I  FG  LSE    KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10  FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69

Query: 64  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70  PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129

Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189

Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
           QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN  L+QC L G E+CAPFIL V P 
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249

Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
           K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309

Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
           P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369

Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q 
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQN 429

Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
           PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN  +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489

Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
           HDFWVLGYGEGKF    DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549

Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           PHLHMGMGVVFAEGVH VG +P + L CG T   L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYP 584

[Close]

BLAST of MC06g0921 vs. ExPASy TrEMBL
Match: Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 972 bits (2514), Expect = 0.0
Identity = 459/580 (79.14%), Postives = 499/580 (86.03%), Query Frame = 0


Query: 6   EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
           +PF     ++FFVL   FG    LSE    KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7   KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66

Query: 66  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67  PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126

Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186

Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
           QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P 
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246

Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
           KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306

Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
           PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366

Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q 
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426

Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
           PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN   SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486

Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
           HDFWVLGYGEGKFS   D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546

Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           PHLHMGMGV+FAEGVHKVG +P   + CG T   ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYP 584

[Close]

BLAST of MC06g0921 vs. ExPASy TrEMBL
Match: A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 458/580 (78.97%), Postives = 498/580 (85.86%), Query Frame = 0


Query: 6   EPFLGPFLVIFFVLISAFG---ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 65
           +PF     ++FFVL   FG    LSE    KIRH++WEVEYMFWSPDCVEN+VMGIN QF
Sbjct: 7   KPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQF 66

Query: 66  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 125
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 67  PGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 126

Query: 126 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 185
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 127 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHK 186

Query: 186 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 245
           QEVGLSS P RWIGEPQS+LINGRGQF+CSIAAKY N L+QC L G EQCAPFIL V P 
Sbjct: 187 QEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPK 246

Query: 246 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 305
           K YRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQN
Sbjct: 247 KTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQN 306

Query: 306 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 365
           PSENYWVS+GVR R P TPPGLT+LNY PNS+SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 307 PSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIF 366

Query: 366 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 425
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++KFRL+ AF+Q 
Sbjct: 367 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQN 426

Query: 426 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 485
           PPPETFP DYDI+ PP NP T T +GVY+ ++G TVDVILQN+N LN   SEIHPWHLHG
Sbjct: 427 PPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLHG 486

Query: 486 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 545
           HDFWVLGYGEGKFS   D +KLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 487 HDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 546

Query: 546 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           PHLHMGMGV+FAEGVHKVG +P   + CG T   ++K+ P
Sbjct: 547 PHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYP 584

[Close]

BLAST of MC06g0921 vs. ExPASy TrEMBL
Match: E7BBM8 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1)

HSP 1 Score: 964 bits (2492), Expect = 0.0
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0


Query: 4   FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
           F +PFL  FLV+   +I  FG  LSE    KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10  FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69

Query: 64  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70  PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129

Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189

Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
           QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN  L+QC L G E+CAPFIL V P 
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249

Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
           K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309

Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
           P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369

Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q 
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQS 429

Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
           PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN  +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489

Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
           HDFWVLGYGEGKF    DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549

Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           PHLHMGMGVVFAEGVH VG +P + L CG T   L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYP 584

[Close]

BLAST of MC06g0921 vs. ExPASy TrEMBL
Match: A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 964 bits (2492), Expect = 0.0
Identity = 458/580 (78.97%), Postives = 501/580 (86.38%), Query Frame = 0


Query: 4   FAEPFLGPFLVIFFVLISAFG-ALSEGS--KIRHHRWEVEYMFWSPDCVENVVMGINHQF 63
           F +PFL  FLV+   +I  FG  LSE    KI+H++W+VEYMFWSPDCVEN+VMGIN +F
Sbjct: 10  FPKPFLS-FLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEF 69

Query: 64  PGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVY 123
           PGPTIRANAGD VVVELTNKLHTEGVVIHWHGILQ GTPWADGTASISQCAINPGETF Y
Sbjct: 70  PGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTY 129

Query: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHR 183
           RFVVDKAGT+FYHGHLGMQRSAGLYGSLIVDP EG  EPFHYDEEINLLLSDWWHQSVH+
Sbjct: 130 RFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHK 189

Query: 184 QEVGLSSIPFRWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPN 243
           QEVGLSS P RWIGEPQS+LING+GQF+CSIAAKYN  L+QC L G E+CAPFIL V P 
Sbjct: 190 QEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPK 249

Query: 244 KIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQN 303
           K YRIRIASTTALASLNFAIGNH+LLVVEADGNYVQPF+T+DIDIYSGE+YSVLI TDQN
Sbjct: 250 KTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQN 309

Query: 304 PSENYWVSVGVRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRIT 363
           P ENYWVS+GVR R P TPPGLTLLNY PNS SKLP S PP TP W+DF RSKNFT RI 
Sbjct: 310 PLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIF 369

Query: 364 AAMGSPRPPATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK 423
           AAMGSP+PP  ++RR+ LLNTQNRING +KWAINNVSL LPPTPYL ++K RLN AF+Q 
Sbjct: 370 AAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQN 429

Query: 424 PPPETFPQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHG 483
           PPPETFP +YDI+ PPPNP TTT +GVY+ ++G TVDVILQN+N LN  +SEIHPWHLHG
Sbjct: 430 PPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHG 489

Query: 484 HDFWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIE 543
           HDFWVLGYGEGKF    DEKKLNLKNPPLRNT VIFPYGWTA+RFVADNPGVWAFHCHIE
Sbjct: 490 HDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 549

Query: 544 PHLHMGMGVVFAEGVHKVGNVPKQVLTCGLTGKTLMKDEP 580
           PHLHMGMGVVFAEGVH VG +P + L CG T   L+K+ P
Sbjct: 550 PHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYP 584

[Close]

BLAST of MC06g0921 vs. ExPASy TrEMBL
Match: A0A6J1H925 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461608 PE=3 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 448/568 (78.87%), Postives = 488/568 (85.92%), Query Frame = 0


Query: 11  PFLVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAG 70
           PFL+I F+   A        KI+H++WEVEYMFWSPDCVEN+VMGIN QFPGPTIRAN G
Sbjct: 8   PFLLILFLFSIAITPSIALPKIKHYKWEVEYMFWSPDCVENIVMGINGQFPGPTIRANVG 67

Query: 71  DTVVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTF 130
           DTVVVELTNKLHTEGVVIHWHGILQ G+PWADGTASISQCAINPGETF YRFVVDKAGTF
Sbjct: 68  DTVVVELTNKLHTEGVVIHWHGILQRGSPWADGTASISQCAINPGETFTYRFVVDKAGTF 127

Query: 131 FYHGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPF 190
           FYHGHLGMQRSAGLYGSLIVDP EG+ EPFHYDEEINLLLSDWWHQSVH+QEVGLSS P 
Sbjct: 128 FYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHKQEVGLSSNPM 187

Query: 191 RWIGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKGSEQCAPFILRVLPNKIYRIRIAST 250
           RWIGEPQ++LINGRGQFNCSIAAKY++  + CALKG EQCAPFI+ V PNK  RIRI+ST
Sbjct: 188 RWIGEPQTILINGRGQFNCSIAAKYHSGSKPCALKGGEQCAPFIMHVHPNKTVRIRISST 247

Query: 251 TALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVG 310
           TALASLNFAIGNH+LLVVEADGNYVQPF T+DIDIYSGE+YSVLI TDQNPSENYWVS+G
Sbjct: 248 TALASLNFAIGNHQLLVVEADGNYVQPFRTSDIDIYSGESYSVLITTDQNPSENYWVSIG 307

Query: 311 VRGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPA 370
           VR R+PNTPPGLTLLNY PNS +KLP   PP TP W+DF RSKNFT RI + M SP+PP 
Sbjct: 308 VRARRPNTPPGLTLLNYLPNSPAKLPILPPPETPDWEDFDRSKNFTFRIFSTMSSPKPPT 367

Query: 371 TFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDY 430
            F RRI LLNTQNR+NG  KWAINNVSL LPPTPYLG++K+RL  AF+Q PPPE FP +Y
Sbjct: 368 RFDRRIFLLNTQNRLNGFTKWAINNVSLALPPTPYLGAMKYRLKTAFNQNPPPENFPSNY 427

Query: 431 DIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGE 490
            I  PPPNPNTT  +GVYR  LG TVDVILQN+N LN   SEIHPWHLHGHDFWVLGYG+
Sbjct: 428 SIDIPPPNPNTTIGNGVYRFKLGETVDVILQNANMLNPNTSEIHPWHLHGHDFWVLGYGD 487

Query: 491 GKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVV 550
           GKFS     +KLNLKNPPLRNT VIFP GWTA+RFVADNPGVWAFHCHIEPHLHMGMGVV
Sbjct: 488 GKFSTAVHGRKLNLKNPPLRNTVVIFPNGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVV 547

Query: 551 FAEGVHKVGNVPKQVLTCGLTGKTLMKD 578
           FAEGV KVG +PK+ L CG+T K LMK+
Sbjct: 548 FAEGVEKVGRIPKKALACGVTAKALMKN 575

[Close]

BLAST of MC06g0921 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 819.7 bits (2116), Expect = 1.5e-237
Identity = 374/565 (66.19%), Postives = 446/565 (78.94%), Query Frame = 0


Query: 14  VIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTV 73
           V + VL+ A    +  + +R + WEVEY +WSPDC E  VM +N +FPGPTI+A AGDT+
Sbjct: 19  VWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTI 78

Query: 74  VVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYH 133
           VV LTNKL TEG+VIHWHGI Q G+PWADG A ++QCAINPGETF Y F V+K GT FYH
Sbjct: 79  VVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYH 138

Query: 134 GHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWI 193
           GH GMQRSAGLYGSLIVD A+G+ E   YD E NLLLSDWWH+++  QE+GLSS P RWI
Sbjct: 139 GHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWI 198

Query: 194 GEPQSLLINGRGQFNCSIAAKYNN--TLQQCALKGSEQCAPFILRVLPNKIYRIRIASTT 253
           GE QS+LINGRGQFNCS+AA+++N  +L  C  K  +QCAP IL V PNK YRIR++STT
Sbjct: 199 GEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTT 258

Query: 254 ALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGV 313
           ALASLN A+  HKL+VVEADGNY+ PF T+DIDIYSGE+YSVL+ TDQ+PS+NY++SVGV
Sbjct: 259 ALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGV 318

Query: 314 RGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPAT 373
           RGR+PNT   LT+LNY     SKLPSS PP TPRWDDF RSKNF+ +I +AMGSP PP  
Sbjct: 319 RGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKK 378

Query: 374 FHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYD 433
           + +R++LLNTQN I+G  KWAINNVSL  P TPYLGS+K+ L   F++K PP ++  DYD
Sbjct: 379 YRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYD 438

Query: 434 IHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEG 493
           I  PPP PNTTT +G+Y      TVDVI+QN+N L   VSEIHPWHLHGHDFWVLGYG+G
Sbjct: 439 IMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDG 498

Query: 494 KFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVF 553
           KF  G DEK  NLKNPPLRNTA+++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVF
Sbjct: 499 KFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVF 558

Query: 554 AEGVHKVGNVPKQVLTCGLTGKTLM 577
           AEG++++G VP + L CGLT + LM
Sbjct: 559 AEGLNRIGKVPDEALGCGLTKQFLM 583

[Close]

BLAST of MC06g0921 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 758.8 bits (1958), Expect = 3.1e-219
Identity = 358/564 (63.48%), Postives = 425/564 (75.35%), Query Frame = 0


Query: 18  VLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTVVVEL 77
           V++ AF + S  + +    WEVEY +W PDC E +VM IN QFPGPTI A AGDTV++ +
Sbjct: 11  VVVVAFHSAS--AAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHV 70

Query: 78  TNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYHGHLG 137
            NKL TEGVVIHWHGI Q GTPWADG A ++QC INPGETF Y+F+VDKAGT FYHGH G
Sbjct: 71  VNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYG 130

Query: 138 MQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWIGEPQ 197
           MQRS+GLYG LIV      KE   YD E NLLLSDWWHQS+H QE+ LSS P RWIGEPQ
Sbjct: 131 MQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQ 190

Query: 198 SLLINGRGQFNCSIAAKYNNTLQQ--CALKGSEQCAPFILRVLPNKIYRIRIASTTALAS 257
           SLLINGRGQFNCS AA +N   ++  C  K ++QCAP  LRV PN++YR+RIASTTALAS
Sbjct: 191 SLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALAS 250

Query: 258 LNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRGRQ 317
           LN A+  H+L+VVEADGNYV PF  NDID+YSGE YSVL+ T+  PS+ YW+SVGVRGR+
Sbjct: 251 LNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGRE 310

Query: 318 PNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFHRR 377
           P TP  LT++NY   + S+ PS  PP TP W+D  RSK+F+ +I AA G P+PP   H +
Sbjct: 311 PKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ 370

Query: 378 IVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQD-YDIHR 437
           ++LLNTQN      KW+INNVSL++P TPYLGSI++ L +A+D K P +    D YDI +
Sbjct: 371 LILLNTQNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMK 430

Query: 438 PPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKFS 497
           PPPNPNTT  SG+Y    G  VDVILQN+N L   +SEIHPWH+HGHDFWVLGYGEGKF 
Sbjct: 431 PPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFK 490

Query: 498 GGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEG 557
            G DEK  NLKNPPLRNT V++P+GWTA+RFV DNPGVW FHCHIEPHLHMGMGVVF EG
Sbjct: 491 PGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEG 550

Query: 558 VHKVG--NVPKQVLTCGLTGKTLM 577
           V ++G   +P + L CGLT K LM
Sbjct: 551 VDRIGKMEIPDEALGCGLTRKWLM 568

[Close]

BLAST of MC06g0921 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 746.9 bits (1927), Expect = 1.2e-215
Identity = 342/520 (65.77%), Postives = 408/520 (78.46%), Query Frame = 0


Query: 14  VIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDTV 73
           V + VL+ A    +  + +R + WEVEY +WSPDC E  VM +N +FPGPTI+A AGDT+
Sbjct: 19  VWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTI 78

Query: 74  VVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFYH 133
           VV LTNKL TEG+VIHWHGI Q G+PWADG A ++QCAINPGETF Y F V+K GT FYH
Sbjct: 79  VVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYH 138

Query: 134 GHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRWI 193
           GH GMQRSAGLYGSLIVD A+G+ E   YD E NLLLSDWWH+++  QE+GLSS P RWI
Sbjct: 139 GHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWI 198

Query: 194 GEPQSLLINGRGQFNCSIAAKYNN--TLQQCALKGSEQCAPFILRVLPNKIYRIRIASTT 253
           GE QS+LINGRGQFNCS+AA+++N  +L  C  K  +QCAP IL V PNK YRIR++STT
Sbjct: 199 GEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTT 258

Query: 254 ALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGV 313
           ALASLN A+  HKL+VVEADGNY+ PF T+DIDIYSGE+YSVL+ TDQ+PS+NY++SVGV
Sbjct: 259 ALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGV 318

Query: 314 RGRQPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPAT 373
           RGR+PNT   LT+LNY     SKLPSS PP TPRWDDF RSKNF+ +I +AMGSP PP  
Sbjct: 319 RGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKK 378

Query: 374 FHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYD 433
           + +R++LLNTQN I+G  KWAINNVSL  P TPYLGS+K+ L   F++K PP ++  DYD
Sbjct: 379 YRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYD 438

Query: 434 IHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEG 493
           I  PPP PNTTT +G+Y      TVDVI+QN+N L   VSEIHPWHLHGHDFWVLGYG+G
Sbjct: 439 IMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDG 498

Query: 494 KFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPG 532
           KF  G DEK  NLKNPPLRNTA+++PYGWTA+RFV DNPG
Sbjct: 499 KFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of MC06g0921 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 598.6 bits (1542), Expect = 5.4e-171
Identity = 290/569 (50.97%), Postives = 371/569 (65.20%), Query Frame = 0


Query: 13  LVIFFVLISAFGALSEGSKIRHHRWEVEYMFWSPDCVENVVMGINHQFPGPTIRANAGDT 72
           +V+ F+ +     L +G KIR  +WEV+Y F SPDC E +V+ IN +FPGPTI+A  GDT
Sbjct: 17  MVLCFIALFFSSVLCQG-KIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 76

Query: 73  VVVELTNKLHTEGVVIHWHGILQHGTPWADGTASISQCAINPGETFVYRFVVDKAGTFFY 132
           +VVEL N   TE V +HWHGI Q GTPW DG   ++QC I PGE F+Y+FVVD+ GT+ Y
Sbjct: 77  IVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMY 136

Query: 133 HGHLGMQRSAGLYGSLIVDPAEGEKEPFHYDEEINLLLSDWWHQSVHRQEVGLSSIPFRW 192
           H H GMQR +GL G + V P   E EPF YD + N LL+DW+H+S+  +  GL+SIPF+W
Sbjct: 137 HSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKW 196

Query: 193 IGEPQSLLINGRGQFNCSIAAKYNNTLQQCALKG-----SEQCAPFILRVLPNKIYRIRI 252
           +GEPQSL+I GRG+FNCS     N T     + G     +  C+ FIL V+P K YR+RI
Sbjct: 197 VGEPQSLMIQGRGRFNCS----NNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRI 256

Query: 253 ASTTALASLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWV 312
            S TAL++L+F I  H L VVEADG+YV+PF   ++ +YSGE YSVL+  DQNP  NYW+
Sbjct: 257 GSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWI 316

Query: 313 SVGVRGRQPNTPPGLTLLNYGPNSISKLP--SSAPPPTPRWDDFARSKNFTTRITAAMGS 372
           +  +  R   TPP   +LNY PN   + P  S +    P W+D       +  I A  G 
Sbjct: 317 TSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGF 376

Query: 373 PRP-PATFHRRIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQK-PPP 432
               P    + IVLLNTQN +NG  +W++NNVS   P TPYL ++K  L  AFD +   P
Sbjct: 377 IHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAP 436

Query: 433 ETF-PQDYDIHRPPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHD 492
           E +  ++YDI   P N N TTS G+YRL   STVDVILQN+N +N   SE HPWHLHGHD
Sbjct: 437 ENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHD 496

Query: 493 FWVLGYGEGKFSGGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPH 552
           FWVLGYGEGKF+   D K+ N  +P  +NT  + P+GWTALRF ADNPGVW+FHCHIE H
Sbjct: 497 FWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESH 556

Query: 553 LHMGMGVVFAEGVHKVGNVPKQVLTCGLT 572
             MGMG+VF  G+ KV ++P  ++ CG T
Sbjct: 557 FFMGMGIVFESGIDKVSSLPSSIMGCGQT 580

[Close]

BLAST of MC06g0921 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 556.2 bits (1432), Expect = 3.1e-158
Identity = 255/382 (66.75%), Postives = 306/382 (80.10%), Query Frame = 0


Query: 197 QSLLINGRGQFNCSIAAKYNN--TLQQCALKGSEQCAPFILRVLPNKIYRIRIASTTALA 256
           +S+LINGRGQFNCS+AA+++N  +L  C  K  +QCAP IL V PNK YRIR++STTALA
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 257 SLNFAIGNHKLLVVEADGNYVQPFLTNDIDIYSGEAYSVLINTDQNPSENYWVSVGVRGR 316
           SLN A+  HKL+VVEADGNY+ PF T+DIDIYSGE+YSVL+ TDQ+PS+NY++SVGVRGR
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130

Query: 317 QPNTPPGLTLLNYGPNSISKLPSSAPPPTPRWDDFARSKNFTTRITAAMGSPRPPATFHR 376
           +PNT   LT+LNY     SKLPSS PP TPRWDDF RSKNF+ +I +AMGSP PP  + +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190

Query: 377 RIVLLNTQNRINGAVKWAINNVSLTLPPTPYLGSIKFRLNAAFDQKPPPETFPQDYDIHR 436
           R++LLNTQN I+G  KWAINNVSL  P TPYLGS+K+ L   F++K PP ++  DYDI  
Sbjct: 191 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMN 250

Query: 437 PPPNPNTTTSSGVYRLDLGSTVDVILQNSNALNEKVSEIHPWHLHGHDFWVLGYGEGKFS 496
           PPP PNTTT +G+Y      TVDVI+QN+N L   VSEIHPWHLHGHDFWVLGYG+GKF 
Sbjct: 251 PPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFK 310

Query: 497 GGADEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEG 556
            G DEK  NLKNPPLRNTA+++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVFAEG
Sbjct: 311 PGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEG 370

Query: 557 VHKVGNVPKQVLTCGLTGKTLM 577
           ++++G VP + L CGLT + LM
Sbjct: 371 LNRIGKVPDEALGCGLTKQFLM 392

The following BLAST results are available for this feature:

BLAST of MC06g0921 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14133	1.9e-282	78.97	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P24792	2.2e-278	77.37	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	2.4e-272	78.32	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
Q40588	1.2e-247	69.98	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]
M4DUF2	3.5e-231	65.72	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]

BLAST of MC06g0921 vs. NCBI nr


Match Name	Identity	Description
XP_038878978.1	79.45	L-ascorbate oxidase [Benincasa hispida]	[more]
NP_001284441.1	79.14	L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis me...	[more]
KAA0055636.1	78.97	L-ascorbate oxidase [Cucumis melo var. makuwa]	[more]
XP_023517000.1	79.58	L-ascorbate oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_004149015.1	78.97	L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidas...	[more]

BLAST of MC06g0921 vs. ExPASy TrEMBL


Match Name	Identity	Description
Q9M5B4	79.14	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1	[more]
A0A5A7UMY3	78.97	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G...	[more]
E7BBM8	78.97	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1	[more]
A0A0A0LXB9	78.97	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1	[more]
A0A6J1H925	78.87	L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461608 PE=3 SV=1	[more]

BLAST of MC06g0921 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G21105.1	1.5e-237	66.19	Plant L-ascorbate oxidase	[more]
AT5G21100.1	3.1e-219	63.48	Plant L-ascorbate oxidase	[more]
AT5G21105.3	1.2e-215	65.77	Plant L-ascorbate oxidase	[more]
AT4G39830.1	5.4e-171	50.97	Cupredoxin superfamily protein	[more]
AT5G21105.2	3.1e-158	66.75	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 30..158 e-value: 4.6E-47 score: 161.3
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 160..365 e-value: 1.5E-65 score: 223.1
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 366..578 e-value: 5.2E-75 score: 253.7
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 29..176
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 159..364
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 369..568
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 32..571 e-value: 2.8E-304 score: 1007.8
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 438..554 e-value: 1.1E-35 score: 122.4
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 39..153 e-value: 1.2E-40 score: 138.3
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 166..329 e-value: 2.5E-45 score: 154.4
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 25..572
None	No IPR available	PANTHER	PTHR11709:SF394	PLANT L-ASCORBATE OXIDASE	coord: 25..572
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 536..547
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 531..551
IPR034259	Ascorbate oxidase, first cupredoxin domain	CDD	cd13845	CuRO_1_AAO	coord: 33..152 e-value: 1.06007E-81 score: 249.67
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 374..562 e-value: 3.33859E-82 score: 252.341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0921.1	MC06g0921.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity