MC06g0473 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0473
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMannosyltransferase
LocationMC06: 3883042 .. 3886509 (-)
RNA-Seq ExpressionMC06g0473
SyntenyMC06g0473
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAACCATTTCTCAAAGAGAACCAAAGTTCAAATAGATATGACGATTACTACTTTGAGATTGAATTTTTGATTCTGAAAGAAAATTATATATCTGTAAGAAATTATGAGGAACTCGAATGCTGGAAAAATATTTCAAGAAATTGAAGGAATAATTCCAACTTTGTTTTCCTCAAATCCAATTTGTCTGGGCAAAATGTTCAGTTCATGAAGTGACGAATAAAAAGAAAAGAATCACTCCGGCGACATAGAGGACAGGACCGAAAGCTGGAAAAATAAACTAGTCATCAGCCGCCGCTGATTATCAGTTATCATCCCACGCGAACCATCTCCGGTTCAGATTTGCGTCTCAAAGTCTGAAAATTTCGGTTTCGAGAATGATTCAGAAGAGACCCAGGTCTTCAGCTGCAGAATCAAAATCAGACAGAATCGAAGACTCACCAGAATTTCTTGGTTCCGAGAAACCCAATTCTCAAAAGCATGGTCTTTTCTCTTTGCCGAACAAGGTGTTTGTGTTCTGCTTGGCATTTCGAATTGCAAATGCGCTTTTATTGCAGACTTATTTCAACCCAGATGAACACTGGCAAGCCCTAGAAGTTGCCCACGGCATCGCCTTTGGGTAACCTTTCTCTTGGTCCAATCGCTTCCCTGTCATTTTTCTCCTTGCCATTTTCATTGCTATTTCTCTCTGTTCTTACTCTTTCCACTTCTTTTTCCTGGTTTCATGGCTATAATTTATTGGTGAATTAGCTTTTGGAAGCTGTAGAACACTTCATGTAATTCAATTGTGGGTTTCCATTTCGCAGGTATGGCCATCTGACGTGGGAATGGAAGAGGGGAATTCGTAGCTACTTGCATCCTTTGTTGTTTGCTTTTCTTTACAAAGTTCTGGCTCTACTCGGCCTTGATACTCCATTGTTCATGGTAAAATGTTAAACTGCATACTTAAACTACTGAAACTTTTATATTGCCTCTGGCAAATTGAATTGTGAAATCCTTGACTATTATATTTGATCAGGTGGGCTTAGATTTTGAGAGATCGACTTTATTCAATTCCATAACACATCTCCAGAAATTAGTTTTACATTGTTGCAAGTGAAATGACATCGATCTTTTGATGGAGTTTCTTTGTTATTGGTCTTTCCCTTAATTTGATTTTATACTCTCTTCACTATGCTTCATAGATGAAAGCTCCGAGGCTGATGCAGTCCATATTCTCAGCTATTGGTGATTTTTACTTGTACAAACTCTCCAGTGTCCTCTTTGGGGATTACGTTGCAAAATGGGCAGTATCCTTCTGTTGGCGATTGGCATGTATATAATCTTCAGTTTCTCTCTTTCCATCTTGGAACTTTCATCCAATCATATCTTCTTAATGAAAAAAGGAAAGAAATGTGTTGTGGTTTTTAAACATGATATACTTAACTCTATTCCAGCTGTTTTCCCAACTCACAAACTGGTTTATGTTCTTCTGCTTCAATCGAACTCTATCAAACAGCTTAGAGACTGTTCTTACACTTGTCAGCTTGTATTATTGGCCTTGTATAAGAATTGCTCCCACCAAATTTTCCAAGGTTTCAAGGAAGTTGGCTTTGTTTATGGCAGCGTTATCATGTGCTGTTCGCCCAACAAGTGCGATTACTTGGCTCTATGTTGGTCTCCTCGAGCTGTGTACGGCACATGATCGACTAAGATTTATCTTCTTAGAGGCTGCTCCTATTGGGTATGTGTCCCATATCCTCCTATATGTTATAGCATGTTTTACTTATTGTTCTTGGTTTCTTGAATAGGTTTGGCTTTTTTCCCATCTTTATGATAGTTTTATTTGAACTTCATTTTTTTAATTAGTGATGTAAAAACCAATATGACAGAGATATTATTTAACAATAGGATTGAATTTCTTATTAATTATGAGATTGTGGTTTAGAGGATGCATTTTTTGCGAGATTGTAAGCATACAGATTATATGAAGAGTCGGTTCCTAATTTAATATGAATGGTGTATTGCGATTTTCTTTTATGGGCTGCCCCTTTGTTTATCTGTTTCCTCATTTGATAAAAAGATCATTAGTCCCCATTGTTTATCTGGATAGACCGTGTCTTATTTTGGGTCAAATAGTTAAAATTGCACCCCGTGGTATATTGAGGAGATTCTCGCACTTATTTTATTAACCTCTCTAAGATTTCATAGTGTTTCCATCTCTCTTATCCCTCTAACTTGTTATTTCAGGACGTTGGTGCTTGGGATTACATGTTTGTTGGATAGATTAATGTATGGCTTCTGGATCTTAGTACCTCTGAATTTTCTAAAGTTCAATGTTCTCTCCTCTGGTGGAGACTTTTATGGAACTCACAAGTGGCACTGGTACTTCACCCAGGGTTTTACTGCCATGCTCTTCAGTTTCGTTCCATTTTCCATTTCTGGCATCATCACCTCGAGAAATTGGAAACTTACAGGTCTTATCATCTGGGTTCTGGGACTTCACAGTCTACTAGGTCATAAAGAATTCAGGTATCTCTTTCAAGGGAAGCTTGTTTCGCTCAATGAAAATCCCGACCTAAATCGGTTTGGCATTAGTTTGAAGTAATGTGTTTGTTATTTACTATGTACAGGTTTGTTCTGCCCATTCTTCCAATAACATTGATGTTCTCTGGCTATTCATTAGCCGCCCTCAGATATCCCGCTTCTCCAAATGGTAGAAGGAAAAAAACGGGAGACGTCCACTCCAAACGCCCTATCAAAATGAAGTTGACCATAATATTTTTGCTAGCTACAAATATCCCAATGGGTTTATACATGAGTTTGGTTCATCAGGTATCTATATTGGCCAATCAGTAAAACAAATATACAAAGTTGGCTGCCATTTTGTTTCTTCTGTTTGATATCTAACCAGCAGACATGGTATGCAGAGAGGAACAGAGGATGTCATGAATCATCTGGCTCGGAAAGCTGTGAATGAGAAAGTCAAAAGCATCCTCTTTTTGATGCCCTGTCATTCAACGCCTTACTATTCCACTCTGCACCAAAATTTACCAATGCGGTTTCTGGACTGCACACCAAGGTGTGTAAACTCGACAAATTACAGTACTCTTTCCCTTGTACTTCTAGTTATTGTTGTTCATTTGTGATGCTGGCTATTTATCTTACAGCGAAGAGAAAGGAATCCCAGTCGAGTCAGACCGTTTCTTGACGAACCCGCTTGATTTTGCAACGGAATTTGCTAAAAATTGGTCTGTCCCAAGTCACATTGTACTGTTTGATTCAGAAGAGAGACTACTCAAGGACTTTCTGATGTCACATTATTTCAAGGAGGTTCGATGTGTTGTATTGTATTTGCTAATCATTAATCATCATGGTTCCTGTTGTCGGAAACTAAACTTTGTGTTGTGTGGAATACAGGATAGGAGATTCTTCCACGCTCACTTCAAGGTGGACCGCGATCTTCAATCTTCGGTGGTTTTGTATGTC

mRNA sequence

CTCAAACCATTTCTCAAAGAGAACCAAAGTTCAAATAGATATGACGATTACTACTTTGAGATTGAATTTTTGATTCTGAAAGAAAATTATATATCTGTAAGAAATTATGAGGAACTCGAATGCTGGAAAAATATTTCAAGAAATTGAAGGAATAATTCCAACTTTGTTTTCCTCAAATCCAATTTGTCTGGGCAAAATGTTCAGTTCATGAAGTGACGAATAAAAAGAAAAGAATCACTCCGGCGACATAGAGGACAGGACCGAAAGCTGGAAAAATAAACTAGTCATCAGCCGCCGCTGATTATCAGTTATCATCCCACGCGAACCATCTCCGGTTCAGATTTGCGTCTCAAAGTCTGAAAATTTCGGTTTCGAGAATGATTCAGAAGAGACCCAGGTCTTCAGCTGCAGAATCAAAATCAGACAGAATCGAAGACTCACCAGAATTTCTTGGTTCCGAGAAACCCAATTCTCAAAAGCATGGTCTTTTCTCTTTGCCGAACAAGGTGTTTGTGTTCTGCTTGGCATTTCGAATTGCAAATGCGCTTTTATTGCAGACTTATTTCAACCCAGATGAACACTGGCAAGCCCTAGAAGTTGCCCACGGCATCGCCTTTGGGTATGGCCATCTGACGTGGGAATGGAAGAGGGGAATTCGTAGCTACTTGCATCCTTTGTTGTTTGCTTTTCTTTACAAAGTTCTGGCTCTACTCGGCCTTGATACTCCATTGTTCATGGTAAAATCTCCGAGGCTGATGCAGTCCATATTCTCAGCTATTGGTGATTTTTACTTGTACAAACTCTCCAGTGTCCTCTTTGGGGATTACGTTGCAAAATGGGCACTGTTTTCCCAACTCACAAACTGGTTTATGTTCTTCTGCTTCAATCGAACTCTATCAAACAGCTTAGAGACTGTTCTTACACTTGTCAGCTTGTATTATTGGCCTTGTATAAGAATTGCTCCCACCAAATTTTCCAAGGTTTCAAGGAAGTTGGCTTTGTTTATGGCAGCGTTATCATGTGCTGTTCGCCCAACAAGTGCGATTACTTGGCTCTATGTTGGTCTCCTCGAGCTGTGTACGGCACATGATCGACTAAGATTTATCTTCTTAGAGGCTGCTCCTATTGGGACGTTGGTGCTTGGGATTACATGTTTGTTGGATAGATTAATGTATGGCTTCTGGATCTTAGTACCTCTGAATTTTCTAAAGTTCAATGTTCTCTCCTCTGGTGGAGACTTTTATGGAACTCACAAGTGGCACTGGTACTTCACCCAGGGTTTTACTGCCATGCTCTTCAGTTTCGTTCCATTTTCCATTTCTGGCATCATCACCTCGAGAAATTGGAAACTTACAGGTCTTATCATCTGGGTTCTGGGACTTCACAGTCTACTAGGTCATAAAGAATTCAGGTTTGTTCTGCCCATTCTTCCAATAACATTGATGTTCTCTGGCTATTCATTAGCCGCCCTCAGATATCCCGCTTCTCCAAATGGTAGAAGGAAAAAAACGGGAGACGTCCACTCCAAACGCCCTATCAAAATGAAGTTGACCATAATATTTTTGCTAGCTACAAATATCCCAATGGGTTTATACATGAGTTTGGTTCATCAGAGAGGAACAGAGGATGTCATGAATCATCTGGCTCGGAAAGCTGTGAATGAGAAAGTCAAAAGCATCCTCTTTTTGATGCCCTGTCATTCAACGCCTTACTATTCCACTCTGCACCAAAATTTACCAATGCGGTTTCTGGACTGCACACCAAGCGAAGAGAAAGGAATCCCAGTCGAGTCAGACCGTTTCTTGACGAACCCGCTTGATTTTGCAACGGAATTTGCTAAAAATTGGTCTGTCCCAAGTCACATTGTACTGTTTGATTCAGAAGAGAGACTACTCAAGGACTTTCTGATGTCACATTATTTCAAGGAGGATAGGAGATTCTTCCACGCTCACTTCAAGGTGGACCGCGATCTTCAATCTTCGGTGGTTTTGTATGTC

Coding sequence (CDS)

ATGATTCAGAAGAGACCCAGGTCTTCAGCTGCAGAATCAAAATCAGACAGAATCGAAGACTCACCAGAATTTCTTGGTTCCGAGAAACCCAATTCTCAAAAGCATGGTCTTTTCTCTTTGCCGAACAAGGTGTTTGTGTTCTGCTTGGCATTTCGAATTGCAAATGCGCTTTTATTGCAGACTTATTTCAACCCAGATGAACACTGGCAAGCCCTAGAAGTTGCCCACGGCATCGCCTTTGGGTATGGCCATCTGACGTGGGAATGGAAGAGGGGAATTCGTAGCTACTTGCATCCTTTGTTGTTTGCTTTTCTTTACAAAGTTCTGGCTCTACTCGGCCTTGATACTCCATTGTTCATGGTAAAATCTCCGAGGCTGATGCAGTCCATATTCTCAGCTATTGGTGATTTTTACTTGTACAAACTCTCCAGTGTCCTCTTTGGGGATTACGTTGCAAAATGGGCACTGTTTTCCCAACTCACAAACTGGTTTATGTTCTTCTGCTTCAATCGAACTCTATCAAACAGCTTAGAGACTGTTCTTACACTTGTCAGCTTGTATTATTGGCCTTGTATAAGAATTGCTCCCACCAAATTTTCCAAGGTTTCAAGGAAGTTGGCTTTGTTTATGGCAGCGTTATCATGTGCTGTTCGCCCAACAAGTGCGATTACTTGGCTCTATGTTGGTCTCCTCGAGCTGTGTACGGCACATGATCGACTAAGATTTATCTTCTTAGAGGCTGCTCCTATTGGGACGTTGGTGCTTGGGATTACATGTTTGTTGGATAGATTAATGTATGGCTTCTGGATCTTAGTACCTCTGAATTTTCTAAAGTTCAATGTTCTCTCCTCTGGTGGAGACTTTTATGGAACTCACAAGTGGCACTGGTACTTCACCCAGGGTTTTACTGCCATGCTCTTCAGTTTCGTTCCATTTTCCATTTCTGGCATCATCACCTCGAGAAATTGGAAACTTACAGGTCTTATCATCTGGGTTCTGGGACTTCACAGTCTACTAGGTCATAAAGAATTCAGGTTTGTTCTGCCCATTCTTCCAATAACATTGATGTTCTCTGGCTATTCATTAGCCGCCCTCAGATATCCCGCTTCTCCAAATGGTAGAAGGAAAAAAACGGGAGACGTCCACTCCAAACGCCCTATCAAAATGAAGTTGACCATAATATTTTTGCTAGCTACAAATATCCCAATGGGTTTATACATGAGTTTGGTTCATCAGAGAGGAACAGAGGATGTCATGAATCATCTGGCTCGGAAAGCTGTGAATGAGAAAGTCAAAAGCATCCTCTTTTTGATGCCCTGTCATTCAACGCCTTACTATTCCACTCTGCACCAAAATTTACCAATGCGGTTTCTGGACTGCACACCAAGCGAAGAGAAAGGAATCCCAGTCGAGTCAGACCGTTTCTTGACGAACCCGCTTGATTTTGCAACGGAATTTGCTAAAAATTGGTCTGTCCCAAGTCACATTGTACTGTTTGATTCAGAAGAGAGACTACTCAAGGACTTTCTGATGTCACATTATTTCAAGGAGGATAGGAGATTCTTCCACGCTCACTTCAAGGTGGACCGCGATCTTCAATCTTCGGTGGTTTTGTATGTC

Protein sequence

MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQTYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMVKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGYSLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPLDFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV
Homology
BLAST of MC06g0473 vs. ExPASy Swiss-Prot
Match: Q94A15 (Mannosyltransferase APTG1 OS=Arabidopsis thaliana OX=3702 GN=APTG1 PE=2 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 8.0e-206
Identity = 348/536 (64.93%), Postives = 421/536 (78.54%), Query Frame = 0

Query: 11  AESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQTYFNPDEHWQ 70
           A+  S  + D P+  G         G    P ++F+FCLAFR+ NALL+QTYFNPDEHWQ
Sbjct: 17  ADGASVNVGDEPDSFG---------GRIGSPRRIFLFCLAFRVVNALLIQTYFNPDEHWQ 76

Query: 71  ALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMVKSPRLMQSI 130
           +LEVAH   FGYG++TWEWKRGIRSYLHP+LFAFLYK+L + GLDTP  M+K+PRLMQSI
Sbjct: 77  SLEVAHRTIFGYGYMTWEWKRGIRSYLHPMLFAFLYKLLQVTGLDTPYIMIKAPRLMQSI 136

Query: 131 FSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWP 190
           FSAIGD YLYKLS  L+G  VA W+LF Q+ NWF+FFC NRT SN LETVLT++ LYYWP
Sbjct: 137 FSAIGDLYLYKLSDALYGGNVATWSLFCQMANWFIFFCLNRTFSNCLETVLTIMGLYYWP 196

Query: 191 CIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPI 250
           CIR +   +  V+RK  L +AAL+CA+RPTSA+ WLYVG+LEL    ++++FI LE  PI
Sbjct: 197 CIRDSSIDY-PVNRKWGLVIAALACAIRPTSAVIWLYVGMLELFLTPNKVKFIILEVIPI 256

Query: 251 GTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFV 310
           G+LVLG TCLLDRLMYG W++VPLNFLKFN LSSGGD+YGTH WHWYFTQGF  MLF+F 
Sbjct: 257 GSLVLGFTCLLDRLMYGSWVIVPLNFLKFNFLSSGGDYYGTHPWHWYFTQGFLVMLFTFT 316

Query: 311 PFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGYSLAALRYPAS 370
           PFSI+GII S+N KL+ LI+WVL ++S+LGHKEFRFVLP+LPI L+FSGY+ A +    S
Sbjct: 317 PFSIAGIIKSKNQKLSALILWVLAIYSILGHKEFRFVLPVLPIALIFSGYAFAQMEVSGS 376

Query: 371 PNG-----RRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARK 430
            +      +++     H+K   K++L++ FLLATNIPM LYMSL HQRGTED MN+L+ +
Sbjct: 377 SSSSSVTKKKQVPRQNHTKWSPKLRLSVYFLLATNIPMALYMSLFHQRGTEDAMNYLSDE 436

Query: 431 AVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPLDFATE 490
           A   +VKSILFLMPCHSTPYYSTLH+N+PM+FLDCTPS EKG   ESD+FL NPL FA+E
Sbjct: 437 AYKGRVKSILFLMPCHSTPYYSTLHRNIPMQFLDCTPSAEKGELDESDQFLVNPLGFASE 496

Query: 491 FAKNWS-VPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 541
            A+NWS  PSHIVLF SEE  L+DF++ H FKE RRFFHAHFKVDRDLQSSVV+YV
Sbjct: 497 LARNWSEPPSHIVLFASEETKLRDFMIQHSFKEVRRFFHAHFKVDRDLQSSVVVYV 542

BLAST of MC06g0473 vs. ExPASy Swiss-Prot
Match: Q9JJQ0 (GPI mannosyltransferase 3 OS=Mus musculus OX=10090 GN=Pigb PE=2 SV=2)

HSP 1 Score: 379.0 bits (972), Expect = 8.8e-104
Identity = 209/526 (39.73%), Postives = 301/526 (57.22%), Query Frame = 0

Query: 9   SAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQTYFNPDEH 68
           S+ E K  R   S +++ +++  S + GL      + +F +A RI N  L+QT F PDE+
Sbjct: 18  SSGEMKLRR-RKSRQYVSAQEKRSPRRGLLGENTYLVLFTIALRILNCFLVQTSFVPDEY 77

Query: 69  WQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMVKSPRLMQ 128
           WQ+LEVAH + F YG+LTWEW   +R Y +PL+FA +YKVL LLG D+  F++  PRL Q
Sbjct: 78  WQSLEVAHRMVFSYGYLTWEWTERLRGYTYPLIFASIYKVLHLLGKDSVQFLIWIPRLGQ 137

Query: 129 SIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYY 188
           ++ SA+ D  LY L   L    VA+W    QL +WF ++C  RTL+N++ET LT ++L+Y
Sbjct: 138 ALLSAVADIRLYSLLKQLENQEVAQWVFLCQLCSWFTWYCCTRTLTNTMETSLTALALFY 197

Query: 189 WPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAA 248
           +P          K S      + AL+C VRPT+ I W+ +          +L        
Sbjct: 198 YPLEGSRSVNSVKYS-----LLVALACVVRPTALIPWVPLLFRHFYQEQRKLHLTLHHFL 257

Query: 249 PIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFS 308
           P+G +   ++ ++DR+ +G W LV LNFLKFNVL + G FYG+H WHWY +QGF  +L +
Sbjct: 258 PVGFITFSLSLIIDRIFFGQWTLVQLNFLKFNVLQNLGTFYGSHPWHWYLSQGFPVVLGT 317

Query: 309 FVPFSISG--IITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGYSLAALR 368
            +PF I G  +   R   L   ++W L ++S+LGHKEFRF+ P+LP  ++F GYSLA L+
Sbjct: 318 HLPFFIHGCFLAPRRLHILLLTVLWTLLVYSMLGHKEFRFIYPVLPFCMVFCGYSLAHLK 377

Query: 369 YPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMNHLAR-- 428
                                  K  + FLL +N+P+  Y  LVHQRGT DVMNH+ +  
Sbjct: 378 --------------------TWRKAALSFLLLSNVPLAFYTGLVHQRGTLDVMNHIQKVC 437

Query: 429 -KAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTP--SEEKGIPVESDRFLTNPLD 488
            +  +    S+  +MPCHSTPYYS +H  L MRFL C P  + +     E+D F  NPL 
Sbjct: 438 PRGPDPASASVFIMMPCHSTPYYSHVHCPLSMRFLQCPPDLTGKTQYLDEADMFYLNPLR 497

Query: 489 F-ATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHF 527
           +   EF  N S+P+H+V F+  E+ +  FL S  ++    FFH H+
Sbjct: 498 WLQQEFHSNASLPTHLVTFNVLEKEINTFLTSGNYERAATFFHTHW 517

BLAST of MC06g0473 vs. ExPASy Swiss-Prot
Match: Q92521 (GPI mannosyltransferase 3 OS=Homo sapiens OX=9606 GN=PIGB PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.1e-103
Identity = 208/524 (39.69%), Postives = 298/524 (56.87%), Query Frame = 0

Query: 24  FLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQTYFNPDEHWQALEVAHGIAFGYG 83
           F   EK   ++  L      + +F +A RI N  L+QT F PDE+WQ+LEV+H + F YG
Sbjct: 43  FNTQEKSARRRGDLLGENIYLLLFTIALRILNCFLVQTSFVPDEYWQSLEVSHHMVFNYG 102

Query: 84  HLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMVKSPRLMQSIFSAIGDFYLYKLS 143
           +LTWEW   +RSY +PL+FA +YK+L LLG D+   ++  PRL Q++ SA+ D  LY L 
Sbjct: 103 YLTWEWTERLRSYTYPLIFASIYKILHLLGKDSVQLLIWIPRLAQALLSAVADVRLYSLM 162

Query: 144 SVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWPCIRIAPTKFSKVS 203
             L    VA+W  F QL +WF ++C  RTL+N++ETVLT+++L+Y+P          K S
Sbjct: 163 KQLENQEVARWVFFCQLCSWFTWYCCTRTLTNTMETVLTIIALFYYPLEGSKSMNSVKYS 222

Query: 204 RKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPIGTLVLGITCLLDR 263
                 + AL+  +RPT+ I W  +     C    +L  I     P+G + L ++ ++DR
Sbjct: 223 S-----LVALAFIIRPTAVILWTPLLFRHFCQEPRKLDLILHHFLPVGFVTLSLSLMIDR 282

Query: 264 LMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFVPFSISG--IITSR 323
           + +G W LV  NFLKFNVL + G FYG+H WHWYF+QGF  +L + +PF I G  +   R
Sbjct: 283 IFFGQWTLVQFNFLKFNVLQNWGTFYGSHPWHWYFSQGFPVILGTHLPFFIHGCYLAPKR 342

Query: 324 NWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGYSLAALRYPASPNGRRKKTGDV 383
              L   ++W L ++S+L HKEFRF+ P+LP  ++F GYSL  L+    P          
Sbjct: 343 YRILLVTVLWTLLVYSMLSHKEFRFIYPVLPFCMVFCGYSLTHLKTWKKP---------- 402

Query: 384 HSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARKAVNEKVK---SILFLM 443
                      + FL  +N+ + LY  LVHQRGT DVM+H+ +   N   K   SI  +M
Sbjct: 403 ----------ALSFLFLSNLFLALYTGLVHQRGTLDVMSHIQKVCYNNPNKSSASIFIMM 462

Query: 444 PCHSTPYYSTLHQNLPMRFLDCTP--SEEKGIPVESDRFLTNPLDFA-TEFAKNWSVPSH 503
           PCHSTPYYS +H  LPMRFL C P  + +     E+D F  NPL++   EF  + S+P+H
Sbjct: 463 PCHSTPYYSHVHCPLPMRFLQCPPDLTGKSHYLDEADVFYLNPLNWLHREFHDDASLPTH 522

Query: 504 IVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLY 540
           ++ F   E  +  FL+S  +K    FFH H    R + S + +Y
Sbjct: 523 LITFSILEEEISAFLISSNYKRTAVFFHTHLPEGR-IGSHIYVY 540

BLAST of MC06g0473 vs. ExPASy Swiss-Prot
Match: Q1LZA0 (GPI mannosyltransferase 3 OS=Bos taurus OX=9913 GN=PIGB PE=2 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 5.7e-103
Identity = 200/493 (40.57%), Postives = 289/493 (58.62%), Query Frame = 0

Query: 46  VFCLAFRIANALLLQTYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFL 105
           +F +A RI N  L+QT F PDE+WQ+LEVAH + F YG+LTWEW   +R Y +PL+FA +
Sbjct: 55  LFIIALRICNCFLVQTSFVPDEYWQSLEVAHRMVFNYGYLTWEWTERLRGYTYPLIFASI 114

Query: 106 YKVLALLGLDTPLFMVKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFM 165
           YK+L LLG D+   ++  PRL Q++ SAI D  LY L   L    VA+W  F QL +WF 
Sbjct: 115 YKILHLLGKDSVHLLIWIPRLAQALLSAIADLRLYSLMKQLENQQVARWVFFCQLCSWFT 174

Query: 166 FFCFNRTLSNSLETVLTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITW 225
           ++C  RTL+N++ETVLT+++L+Y+P          K S      + AL+  +RPT+ I W
Sbjct: 175 WYCCTRTLTNTMETVLTIIALFYYPLEGSKSMNSVKYSS-----LVALAFIIRPTAVIPW 234

Query: 226 LYVGLLELCTAHDRLRFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSG 285
           + +          +L  I  +  P+G + L ++ ++DR+ +G W LV  NFLKFNVL   
Sbjct: 235 IPLLFRHFWQEQRKLDLILHQFLPVGFVTLSLSLIIDRIFFGQWTLVQYNFLKFNVLQDL 294

Query: 286 GDFYGTHKWHWYFTQGFTAMLFSFVPFSISG-IITSRNWKL-TGLIIWVLGLHSLLGHKE 345
           G FYG+H WHWYF+QGF A+L + +PF I G  +  + +++    ++W L ++S+L HKE
Sbjct: 295 GSFYGSHPWHWYFSQGFPAVLGTHLPFFIHGCFLAPKRYRIFLVTVLWTLLVYSMLSHKE 354

Query: 346 FRFVLPILPITLMFSGYSLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPM 405
           FRF+ P+LP  ++F GYSL  L+    P                     + FL  +N+ +
Sbjct: 355 FRFIYPVLPFCMVFCGYSLNNLKTWKKP--------------------ALSFLFLSNMLL 414

Query: 406 GLYMSLVHQRGTEDVMNH---LARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDC 465
            LY  LVHQRGT DVM +   L+    N    S+L +MPCHSTPYYS +H  LPMRFL C
Sbjct: 415 ALYTGLVHQRGTLDVMTNIQELSYNNTNVSSASVLMMMPCHSTPYYSHVHYPLPMRFLQC 474

Query: 466 TP--SEEKGIPVESDRFLTNPLDFA-TEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKE 525
            P  + +    VE+D F  NPL +   EF  +  +P+H+++F   E  +  FL+S+ ++ 
Sbjct: 475 PPDLTGKTDYLVEADMFYLNPLKWLYMEFQNDSQLPTHLIMFSVLEEEISPFLISNNYER 522

Query: 526 DRRFFHAHFKVDR 531
              FFH HF   R
Sbjct: 535 TAVFFHTHFPESR 522

BLAST of MC06g0473 vs. ExPASy Swiss-Prot
Match: Q7SXZ1 (GPI mannosyltransferase 3 OS=Danio rerio OX=7955 GN=pigb PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.1e-98
Identity = 214/547 (39.12%), Postives = 311/547 (56.86%), Query Frame = 0

Query: 2   IQKRPRSSAAESKSDRIEDSPEFL-GSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 61
           I++R + S + ++S R+      L  ++    Q   +F +   + VF +  R+ N +L+Q
Sbjct: 4   IRERFKQSKSSNESVRLRKRSSILYSTDDRKLQTLKIFGI--NITVFIVLVRLLNCVLVQ 63

Query: 62  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 121
           T F PDE WQ+LE++H + FGYG+ TWEWK GIR Y +PLLFA +YK+L L+  DT   +
Sbjct: 64  TSFVPDEFWQSLEISHRMVFGYGYETWEWKEGIRGYSYPLLFALMYKLLQLISYDTVYLL 123

Query: 122 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 181
           V  PR+ Q++ +A  D  LYKL        VAKW  F QL +WF +FC +RTL+N+ ET 
Sbjct: 124 VFVPRVFQALLAAYADVKLYKLILQWETPDVAKWTCFCQLCSWFAWFCCSRTLTNTTETA 183

Query: 182 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 241
           LT ++L ++P     P   +  S K  L + +L+  +RPT+ I W  +     CT  D+L
Sbjct: 184 LTTLALCFYP----LPGSKTHSSWKY-LTLVSLAVVIRPTALIVWFPLIFYHFCTDQDKL 243

Query: 242 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 301
           + I     PIG L LG++ L+D   YG WI V  NFLKFNVL + G+FYG+H WHWY+TQ
Sbjct: 244 KLITHRYFPIGVLALGVSTLIDSFFYGKWIFVQWNFLKFNVLHNVGEFYGSHPWHWYWTQ 303

Query: 302 GFTAMLFSFVPFSISGII--TSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFS 361
           G   ++   +P  + G +  T ++  L   IIW   ++SLL HKEFRF+ P+LP  ++F 
Sbjct: 304 GLPVVIGPHLPLVLHGCLLSTRKHTILLLTIIWTTAVYSLLAHKEFRFIYPVLPFCMIFC 363

Query: 362 GYSLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDV 421
           G SLA L+       R+   G                LL  N+   LY  LVHQRG  DV
Sbjct: 364 GLSLAKLQ-----AWRKAAAG---------------ALLLFNLCPALYTGLVHQRGALDV 423

Query: 422 MNHLARKAVNEKVKS---ILFLMPCHSTPYYSTLHQNLPMRFLDCTP--SEEKGIPVESD 481
           M+ L    V+ +  +   +LFLMPCHSTP YS LH  L +RFL+C P  +  +    E+D
Sbjct: 424 MHVLQPLCVSSQSPNPPELLFLMPCHSTPLYSHLHCPLKLRFLECPPDLTGRQDYMDEAD 483

Query: 482 RFLTNPLDFA-TEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDL 540
            F ++PL +  T F    ++PSH+VLFDS ++ +  FL  + F +    FH HF   R +
Sbjct: 484 VFFSDPLHWLNTSFPLRSTLPSHLVLFDSLQKEISSFLEENSFAKQAEIFHTHFPEGR-V 522

BLAST of MC06g0473 vs. NCBI nr
Match: XP_022135464.1 (GPI mannosyltransferase 3 [Momordica charantia])

HSP 1 Score: 1087 bits (2812), Expect = 0.0
Identity = 538/540 (99.63%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ
Sbjct: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL
Sbjct: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY
Sbjct: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL
Sbjct: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. NCBI nr
Match: XP_038884088.1 (GPI mannosyltransferase 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 466/540 (86.30%), Postives = 501/540 (92.78%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M+QKRPR+S A SK  RIEDSPEFL SEKP S+K+ LFS  NKV V CLAFR ANALLLQ
Sbjct: 1   MVQKRPRTSVAASKLVRIEDSPEFLRSEKPKSKKYDLFSSTNKVLVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH I FGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLG+DTPL M
Sbjct: 61  TYFNPDEHWQALEVAHNITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGIDTPLIM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLQSIFSAVGDFYLYRLSGALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL SLYYWPCI +A T++SKVSRKLALFMAALSCA+RPTSA TWLYVGLLEL +AHDRL
Sbjct: 181 LTLASLYYWPCISVATTRYSKVSRKLALFMAALSCAIRPTSAATWLYVGLLELFSAHDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDRLMYG WILVP NFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRLMYGSWILVPFNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GFTAMLFSF+PFSISGIITS+ WKL+GLIIWVLG+HSLLGHKEFRFVLPILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIITSKRWKLSGLIIWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRY ASPNG+R+K+ D+H+KRP+KM L II LLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYHASPNGKREKSRDLHTKRPMKMNLAIILLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLAR+A  EKVK ILFLMPCHSTPYYSTLH+NLPM+FLDCTPSEE  IPVESDRFLTNPL
Sbjct: 421 HLAREAATEKVKRILFLMPCHSTPYYSTLHRNLPMQFLDCTPSEENRIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
            FATEFAKNW+VPSHIVLFDSEER+LK+FL+SH FKED+RFFH+HFKVDRDLQSSVVLYV
Sbjct: 481 AFATEFAKNWTVPSHIVLFDSEERVLKEFLISHSFKEDKRFFHSHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. NCBI nr
Match: XP_038884087.1 (GPI mannosyltransferase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 466/541 (86.14%), Postives = 501/541 (92.61%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M+QKRPR+S A SK  RIEDSPEFL SEKP S+K+ LFS  NKV V CLAFR ANALLLQ
Sbjct: 1   MVQKRPRTSVAASKLVRIEDSPEFLRSEKPKSKKYDLFSSTNKVLVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFG-YGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLF 120
           TYFNPDEHWQALEVAH I FG YGHLTWEWKRGIRSYLHPLLFAFLYKVLALLG+DTPL 
Sbjct: 61  TYFNPDEHWQALEVAHNITFGRYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGIDTPLI 120

Query: 121 MVKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLET 180
           M+K+PRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLET
Sbjct: 121 MIKAPRLLQSIFSAVGDFYLYRLSGALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLET 180

Query: 181 VLTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDR 240
           VLTL SLYYWPCI +A T++SKVSRKLALFMAALSCA+RPTSA TWLYVGLLEL +AHDR
Sbjct: 181 VLTLASLYYWPCISVATTRYSKVSRKLALFMAALSCAIRPTSAATWLYVGLLELFSAHDR 240

Query: 241 LRFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFT 300
           LRFIFLEAAPIG+LVLGIT LLDRLMYG WILVP NFLKFNVLSSGGDFYGTHKWHWYFT
Sbjct: 241 LRFIFLEAAPIGSLVLGITFLLDRLMYGSWILVPFNFLKFNVLSSGGDFYGTHKWHWYFT 300

Query: 301 QGFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSG 360
           QGFTAMLFSF+PFSISGIITS+ WKL+GLIIWVLG+HSLLGHKEFRFVLPILPI LMFSG
Sbjct: 301 QGFTAMLFSFIPFSISGIITSKRWKLSGLIIWVLGIHSLLGHKEFRFVLPILPIALMFSG 360

Query: 361 YSLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVM 420
           YSLAALRY ASPNG+R+K+ D+H+KRP+KM L II LLATNIPMGLYMSLVHQRGTEDVM
Sbjct: 361 YSLAALRYHASPNGKREKSRDLHTKRPMKMNLAIILLLATNIPMGLYMSLVHQRGTEDVM 420

Query: 421 NHLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNP 480
           NHLAR+A  EKVK ILFLMPCHSTPYYSTLH+NLPM+FLDCTPSEE  IPVESDRFLTNP
Sbjct: 421 NHLAREAATEKVKRILFLMPCHSTPYYSTLHRNLPMQFLDCTPSEENRIPVESDRFLTNP 480

Query: 481 LDFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLY 540
           L FATEFAKNW+VPSHIVLFDSEER+LK+FL+SH FKED+RFFH+HFKVDRDLQSSVVLY
Sbjct: 481 LAFATEFAKNWTVPSHIVLFDSEERVLKEFLISHSFKEDKRFFHSHFKVDRDLQSSVVLY 540

BLAST of MC06g0473 vs. NCBI nr
Match: XP_023535348.1 (GPI mannosyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 466/540 (86.30%), Postives = 501/540 (92.78%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  I+DSPEFL SEKP SQ +  F   NK+FV CLAFR ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIDDSPEFLPSEKPKSQTYDDFCSTNKLFVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH IAFGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHRIAFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLFM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRL++SIFSA+GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAVGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIR+APT+FSKV RK+ALFMAALSCAVRPTSAITWLYVG+LEL +A DRL
Sbjct: 181 LTLVSLYYWPCIRVAPTRFSKVWRKMALFMAALSCAVRPTSAITWLYVGMLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GFTAMLFSF+PFSISGII+S+ WKLTGLIIWVLG+HSLLGHKEFRFVLPILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIISSKKWKLTGLIIWVLGVHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNG+R+K+ DVHSKRP+KMKL +IFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGKREKSRDVHSKRPMKMKLAVIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEEK IPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+  SHIVLF+SEER LKDFL SH FKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTETSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. NCBI nr
Match: XP_022936689.1 (GPI mannosyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 462/540 (85.56%), Postives = 500/540 (92.59%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  I+DSPEFL SEKP S+ +  F   NK+ V CLAFR ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIDDSPEFLPSEKPKSKTYDDFCSTNKLLVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH IAFGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHRIAFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLFM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRL++SIFSA GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAAGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIR+APT+ SKVSRK+ALFMAALSCA+RPTSAITWLYVG+LEL +A DRL
Sbjct: 181 LTLVSLYYWPCIRVAPTRSSKVSRKMALFMAALSCAIRPTSAITWLYVGMLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GFTAMLFSF+PFSISGII+S+ WKLTGL+IWVLG+HSLLGHKEFRFVLPILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIISSKKWKLTGLVIWVLGVHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNG+R+K+ DVHSK P+KMKL +IFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGKREKSRDVHSKCPMKMKLAVIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEE+ IPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEREIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+VPSHIVLF+SEER LKDFL SH FKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTVPSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. ExPASy TrEMBL
Match: A0A6J1C1I0 (Mannosyltransferase OS=Momordica charantia OX=3673 GN=LOC111007416 PE=3 SV=1)

HSP 1 Score: 1087 bits (2812), Expect = 0.0
Identity = 538/540 (99.63%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ
Sbjct: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL
Sbjct: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY
Sbjct: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL
Sbjct: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. ExPASy TrEMBL
Match: A0A6J1F8Z8 (Mannosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443205 PE=3 SV=1)

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 462/540 (85.56%), Postives = 500/540 (92.59%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  I+DSPEFL SEKP S+ +  F   NK+ V CLAFR ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIDDSPEFLPSEKPKSKTYDDFCSTNKLLVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH IAFGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHRIAFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLFM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRL++SIFSA GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAAGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIR+APT+ SKVSRK+ALFMAALSCA+RPTSAITWLYVG+LEL +A DRL
Sbjct: 181 LTLVSLYYWPCIRVAPTRSSKVSRKMALFMAALSCAIRPTSAITWLYVGMLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GFTAMLFSF+PFSISGII+S+ WKLTGL+IWVLG+HSLLGHKEFRFVLPILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIISSKKWKLTGLVIWVLGVHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNG+R+K+ DVHSK P+KMKL +IFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGKREKSRDVHSKCPMKMKLAVIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEE+ IPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEREIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+VPSHIVLF+SEER LKDFL SH FKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTVPSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. ExPASy TrEMBL
Match: A0A6J1IFD5 (Mannosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111473821 PE=3 SV=1)

HSP 1 Score: 940 bits (2429), Expect = 0.0
Identity = 460/540 (85.19%), Postives = 497/540 (92.04%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  IEDSPEFL SE+  S+ +  F   NK+FV CLAFR+ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIEDSPEFLPSEELKSKTYDDFCSTNKLFVLCLAFRVANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH I FGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPL M
Sbjct: 61  TYFNPDEHWQALEVAHRITFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLLM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRL++SIFSA+GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAVGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL+SLYYWPCIR+APT+FSKVSRK+ALFMAALSCAVRPTSAITWLYVGLLEL +A DRL
Sbjct: 181 LTLISLYYWPCIRVAPTRFSKVSRKMALFMAALSCAVRPTSAITWLYVGLLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLG T +LDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGFTFVLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GFTAMLFSF+PFSISGIITSR WKLTGLIIWVLG+HSLLGHKEFRFVLPILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIITSRKWKLTGLIIWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPAS NG+R+K+ D H K P+KMKL ++FLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASLNGKREKSRDAHGKSPMKMKLAVMFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEEK IPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+VPSHIVLF+SEER LKDFL SH FKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTVPSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. ExPASy TrEMBL
Match: A0A0A0LTJ6 (Mannosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G270410 PE=3 SV=1)

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 450/540 (83.33%), Postives = 492/540 (91.11%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M++KRPR+  + +K  R EDSPE L SEK   +++G+F   NKV +FCLAFR ANALLLQ
Sbjct: 1   MVEKRPRTMLSTAKLVRNEDSPELLRSEKRKFKRYGVFFSTNKVLLFCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH I FGYGHLTWEWKRGIRSYLHPLLFA LYK+LALLG+DTPL M
Sbjct: 61  TYFNPDEHWQALEVAHDITFGYGHLTWEWKRGIRSYLHPLLFACLYKLLALLGIDTPLLM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLQSIFSAVGDFYLYRLSGALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL SLYYWPCI +APT+ SKVSRKLALFMAALSCA+RPTSAITWLYVGLLEL +AHD+L
Sbjct: 181 LTLASLYYWPCISVAPTRISKVSRKLALFMAALSCAIRPTSAITWLYVGLLELFSAHDQL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDR+MYG W LVPLNFL+FNV SSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRVMYGSWTLVPLNFLRFNVFSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GF+AMLFSF+PFSISGIITSR WKL+GLI+WVLG+HSLLGHKEFRFVLPILPI LMFSGY
Sbjct: 301 GFSAMLFSFIPFSISGIITSRKWKLSGLIVWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRY  S NG+R K+ D+H+KRP+KM L IIFLL TNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYQTSSNGKRAKSRDLHTKRPMKMNLAIIFLLTTNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLAR+AV  KVK ILFLMPCHSTPYYSTLHQNLPM+FLDCTPS EKG PVES+RFLTNPL
Sbjct: 421 HLAREAVTGKVKRILFLMPCHSTPYYSTLHQNLPMQFLDCTPSVEKGSPVESERFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DF+ EFAKNW+VPSHIVLFDSEERLL DFL+SH FKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFSIEFAKNWTVPSHIVLFDSEERLLMDFLISHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. ExPASy TrEMBL
Match: A0A1S3CU16 (Mannosyltransferase OS=Cucumis melo OX=3656 GN=LOC103504543 PE=3 SV=1)

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 446/540 (82.59%), Postives = 488/540 (90.37%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60
           M+QKRP++    SK  R EDSPE L SEKP S+++ LF   NKV +FCLAFR ANA LLQ
Sbjct: 1   MVQKRPKTLVTTSKLVRNEDSPELLRSEKPKSKRYDLFFSMNKVLLFCLAFRFANAFLLQ 60

Query: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH I FGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLG+DTPL M
Sbjct: 61  TYFNPDEHWQALEVAHYITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGVDTPLLM 120

Query: 121 VKSPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +K+PRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLQSIFSAVGDFYLYRLSRALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL SLYYWPCI + PT+ SKVSRKLALFMAALSC +RPTSAITWLYVGLLEL +A+DRL
Sbjct: 181 LTLASLYYWPCISVVPTRISKVSRKLALFMAALSCGIRPTSAITWLYVGLLELFSANDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDR+MYG W LVPLNFL+FNV SSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRVMYGSWTLVPLNFLRFNVFSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360
           GF+AMLFSF+PFSISGI+ SR WKL+GL++WVLG+HSLLGHKEFRFVLPILPI LMFSGY
Sbjct: 301 GFSAMLFSFIPFSISGIVASRKWKLSGLLVWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALR   SPN +R+K+ ++H+KRP+KM L  IFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRSQTSPNSKREKSRNLHTKRPMKMSLATIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480
           HLAR+A N KVK ILFLMPCHSTPYYSTLHQNLPM+FLDCTPS EKG  VES+RFL NPL
Sbjct: 421 HLAREAANGKVKRILFLMPCHSTPYYSTLHQNLPMQFLDCTPSVEKGSLVESERFLMNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DF+ EFAKNW+VPSHIVLFDSEERLLKDFL+SH FKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFSIEFAKNWTVPSHIVLFDSEERLLKDFLISHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

BLAST of MC06g0473 vs. TAIR 10
Match: AT5G14850.1 (Alg9-like mannosyltransferase family )

HSP 1 Score: 718.0 bits (1852), Expect = 5.7e-207
Identity = 348/536 (64.93%), Postives = 421/536 (78.54%), Query Frame = 0

Query: 11  AESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQTYFNPDEHWQ 70
           A+  S  + D P+  G         G    P ++F+FCLAFR+ NALL+QTYFNPDEHWQ
Sbjct: 17  ADGASVNVGDEPDSFG---------GRIGSPRRIFLFCLAFRVVNALLIQTYFNPDEHWQ 76

Query: 71  ALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMVKSPRLMQSI 130
           +LEVAH   FGYG++TWEWKRGIRSYLHP+LFAFLYK+L + GLDTP  M+K+PRLMQSI
Sbjct: 77  SLEVAHRTIFGYGYMTWEWKRGIRSYLHPMLFAFLYKLLQVTGLDTPYIMIKAPRLMQSI 136

Query: 131 FSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWP 190
           FSAIGD YLYKLS  L+G  VA W+LF Q+ NWF+FFC NRT SN LETVLT++ LYYWP
Sbjct: 137 FSAIGDLYLYKLSDALYGGNVATWSLFCQMANWFIFFCLNRTFSNCLETVLTIMGLYYWP 196

Query: 191 CIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPI 250
           CIR +   +  V+RK  L +AAL+CA+RPTSA+ WLYVG+LEL    ++++FI LE  PI
Sbjct: 197 CIRDSSIDY-PVNRKWGLVIAALACAIRPTSAVIWLYVGMLELFLTPNKVKFIILEVIPI 256

Query: 251 GTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFV 310
           G+LVLG TCLLDRLMYG W++VPLNFLKFN LSSGGD+YGTH WHWYFTQGF  MLF+F 
Sbjct: 257 GSLVLGFTCLLDRLMYGSWVIVPLNFLKFNFLSSGGDYYGTHPWHWYFTQGFLVMLFTFT 316

Query: 311 PFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGYSLAALRYPAS 370
           PFSI+GII S+N KL+ LI+WVL ++S+LGHKEFRFVLP+LPI L+FSGY+ A +    S
Sbjct: 317 PFSIAGIIKSKNQKLSALILWVLAIYSILGHKEFRFVLPVLPIALIFSGYAFAQMEVSGS 376

Query: 371 PNG-----RRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARK 430
            +      +++     H+K   K++L++ FLLATNIPM LYMSL HQRGTED MN+L+ +
Sbjct: 377 SSSSSVTKKKQVPRQNHTKWSPKLRLSVYFLLATNIPMALYMSLFHQRGTEDAMNYLSDE 436

Query: 431 AVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPLDFATE 490
           A   +VKSILFLMPCHSTPYYSTLH+N+PM+FLDCTPS EKG   ESD+FL NPL FA+E
Sbjct: 437 AYKGRVKSILFLMPCHSTPYYSTLHRNIPMQFLDCTPSAEKGELDESDQFLVNPLGFASE 496

Query: 491 FAKNWS-VPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 541
            A+NWS  PSHIVLF SEE  L+DF++ H FKE RRFFHAHFKVDRDLQSSVV+YV
Sbjct: 497 LARNWSEPPSHIVLFASEETKLRDFMIQHSFKEVRRFFHAHFKVDRDLQSSVVVYV 542

BLAST of MC06g0473 vs. TAIR 10
Match: AT5G14850.2 (Alg9-like mannosyltransferase family )

HSP 1 Score: 678.7 bits (1750), Expect = 3.8e-195
Identity = 329/517 (63.64%), Postives = 401/517 (77.56%), Query Frame = 0

Query: 11  AESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQTYFNPDEHWQ 70
           A+  S  + D P+  G         G    P ++F+FCLAFR+ NALL+QTYFNPDEHWQ
Sbjct: 17  ADGASVNVGDEPDSFG---------GRIGSPRRIFLFCLAFRVVNALLIQTYFNPDEHWQ 76

Query: 71  ALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMVKSPRLMQSI 130
           +LEVAH   FGYG++TWEWKRGIRSYLHP+LFAFLYK+L + GLDTP  M+K+PRLMQSI
Sbjct: 77  SLEVAHRTIFGYGYMTWEWKRGIRSYLHPMLFAFLYKLLQVTGLDTPYIMIKAPRLMQSI 136

Query: 131 FSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWP 190
           FSAIGD YLYKLS  L+G  VA W+LF Q+ NWF+FFC NRT SN LETVLT++ LYYWP
Sbjct: 137 FSAIGDLYLYKLSDALYGGNVATWSLFCQMANWFIFFCLNRTFSNCLETVLTIMGLYYWP 196

Query: 191 CIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPI 250
           CIR +   +  V+RK  L +AAL+CA+RPTSA+ WLYVG+LEL    ++++FI LE  PI
Sbjct: 197 CIRDSSIDY-PVNRKWGLVIAALACAIRPTSAVIWLYVGMLELFLTPNKVKFIILEVIPI 256

Query: 251 GTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFV 310
           G+LVLG TCLLDRLMYG W++VPLNFLKFN LSSGGD+YGTH WHWYFTQGF  MLF+F 
Sbjct: 257 GSLVLGFTCLLDRLMYGSWVIVPLNFLKFNFLSSGGDYYGTHPWHWYFTQGFLVMLFTFT 316

Query: 311 PFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGYSLAALRYPAS 370
           PFSI+GII S+N KL+ LI+WVL ++S+LGHKEFRFVLP+LPI L+FSGY+ A +    S
Sbjct: 317 PFSIAGIIKSKNQKLSALILWVLAIYSILGHKEFRFVLPVLPIALIFSGYAFAQMEVSGS 376

Query: 371 PNG-----RRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARK 430
            +      +++     H+K   K++L++ FLLATNIPM LYMSL HQRGTED MN+L+ +
Sbjct: 377 SSSSSVTKKKQVPRQNHTKWSPKLRLSVYFLLATNIPMALYMSLFHQRGTEDAMNYLSDE 436

Query: 431 AVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPLDFATE 490
           A   +VKSILFLMPCHSTPYYSTLH+N+PM+FLDCTPS EKG   ESD+FL NPL FA+E
Sbjct: 437 AYKGRVKSILFLMPCHSTPYYSTLHRNIPMQFLDCTPSAEKGELDESDQFLVNPLGFASE 496

Query: 491 FAKNWS-VPSHIVLFDSEERLLKDFLMSHYFKEDRRF 522
            A+NWS  PSHIVLF SEE  L+DF++ H FKE R F
Sbjct: 497 LARNWSEPPSHIVLFASEETKLRDFMIQHSFKEARGF 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94A158.0e-20664.93Mannosyltransferase APTG1 OS=Arabidopsis thaliana OX=3702 GN=APTG1 PE=2 SV=1[more]
Q9JJQ08.8e-10439.73GPI mannosyltransferase 3 OS=Mus musculus OX=10090 GN=Pigb PE=2 SV=2[more]
Q925211.1e-10339.69GPI mannosyltransferase 3 OS=Homo sapiens OX=9606 GN=PIGB PE=1 SV=1[more]
Q1LZA05.7e-10340.57GPI mannosyltransferase 3 OS=Bos taurus OX=9913 GN=PIGB PE=2 SV=1[more]
Q7SXZ11.1e-9839.12GPI mannosyltransferase 3 OS=Danio rerio OX=7955 GN=pigb PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022135464.10.099.63GPI mannosyltransferase 3 [Momordica charantia][more]
XP_038884088.10.086.30GPI mannosyltransferase 3 isoform X2 [Benincasa hispida][more]
XP_038884087.10.086.14GPI mannosyltransferase 3 isoform X1 [Benincasa hispida][more]
XP_023535348.10.086.30GPI mannosyltransferase 3 [Cucurbita pepo subsp. pepo][more]
XP_022936689.10.085.56GPI mannosyltransferase 3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1C1I00.099.63Mannosyltransferase OS=Momordica charantia OX=3673 GN=LOC111007416 PE=3 SV=1[more]
A0A6J1F8Z80.085.56Mannosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443205 PE=3 SV=1[more]
A0A6J1IFD50.085.19Mannosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111473821 PE=3 SV=1[more]
A0A0A0LTJ60.083.33Mannosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G270410 PE=3 SV=1[more]
A0A1S3CU160.082.59Mannosyltransferase OS=Cucumis melo OX=3656 GN=LOC103504543 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G14850.15.7e-20764.93Alg9-like mannosyltransferase family [more]
AT5G14850.23.8e-19563.64Alg9-like mannosyltransferase family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005599GPI mannosyltransferasePFAMPF03901Glyco_transf_22coord: 44..450
e-value: 7.7E-114
score: 381.2
IPR005599GPI mannosyltransferasePANTHERPTHR22760GLYCOSYLTRANSFERASEcoord: 44..537
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
IPR039521GPI mannosyltransferase 3PANTHERPTHR22760:SF4GPI MANNOSYLTRANSFERASE 3coord: 44..537

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0473.1MC06g0473.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
biological_process GO:0097502 mannosylation
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000026 alpha-1,2-mannosyltransferase activity
molecular_function GO:0004376 glycolipid mannosyltransferase activity
molecular_function GO:0016757 glycosyltransferase activity