MC06g0276 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0276 vs. ExPASy Swiss-Prot
Match: F4KBF3 (Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE17 PE=2 SV=1)

HSP 1 Score: 1048.5 bits (2710), Expect = 3.5e-305
Identity = 503/718 (70.06%), Postives = 602/718 (83.84%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGT-PETWRHISKRVLSPDLP 60
           M YK+L+SIT  DIEALG++ +V+EKL   L +II  HG+ T PETW  IS+R+L P+LP
Sbjct: 1   MAYKSLNSITKSDIEALGISGDVSEKLLRDLEDII--HGSSTPPETWIQISRRILHPNLP 60

Query: 61  FSFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSF 120
           FSFHQMMYYGCYK +GPD PAWIP+P+ A  TNVG+LLE+RGKEFLG NY++P SSFSSF
Sbjct: 61  FSFHQMMYYGCYKDFGPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSFSSF 120

Query: 121 QEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDC 180
           QEFSVSNPEVYW+TVLDE++I FS PP CIL+ + S +    +PGG+WLPGA  NPA++C
Sbjct: 121 QEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGD----NPGGKWLPGAYLNPARNC 180

Query: 181 LSINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDM 240
           L+    R LDD+ I WRDEG D+LPV  +TL ELR++VWL AHAL+++G E+ +AIA+DM
Sbjct: 181 LTNGFKRRLDDIVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVDM 240

Query: 241 PMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLY 300
           PMNV +V+IYLAIVLAGHVVVSIADSF+ REISTRL ISKAKAIFTQD+IIRGDKSIPLY
Sbjct: 241 PMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPLY 300

Query: 301 SRIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNI 360
            R++DA++PLAIV+P   +   MKLR+ D+SW+ FL   ++L+GVE+ A EK   ++TNI
Sbjct: 301 RRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNLRGVEYVAAEKPAGAYTNI 360

Query: 361 LFSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLN 420
           LFSSGTTGEPKAIPWT ++PLK+AADAWCH+D++ GDVVAWPTNLGWMMGPWLVYASL+N
Sbjct: 361 LFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASLIN 420

Query: 421 RASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGE 480
            A M LYNGSPL   F KFVQDA+V++LGVIPSIVR+W+++N TSGYDWS IRCF STGE
Sbjct: 421 GACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGSTGE 480

Query: 481 ASSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGN 540
           AS+IDEYLWLMGRA YKP+IEYCGGTEIGG F++GSLLQ Q+LAAFS  AMGC LFIL  
Sbjct: 481 ASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFILDE 540

Query: 541 DDSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSP 600
           D +PIP    G+GELAL P MFGASS+LLN +H+ VYF+GMP + G ILRRHGDLFER+ 
Sbjct: 541 DSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFERTS 600

Query: 601 RAYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVV 660
           + YYRAHGRADDTMNLGGIKV S+EIER+CN+VD+S+LETAAIGVPP  GGPEQLV+ VV
Sbjct: 601 KGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVV 660

Query: 661 FKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQL 718
           FK+P  ++PDL+ LK SFNS +QK LNPLF+V  VV  PSLPRTATNKVMRRVLRQQL
Sbjct: 661 FKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL 712

BLAST of MC06g0276 vs. ExPASy Swiss-Prot
Match: M4IRL9 (Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 5.0e-203
Identity = 367/722 (50.83%), Postives = 490/722 (67.87%), Query Frame = 0

Query: 9   ITVIDIEALGVASEVAEKLHGLLSEIIR-SHGNGTPETWRH-ISKRVLSPDLPFSFHQMM 68
           + V D+   G+++E A+    +L +I+  + G    E WR  +++R+L P  P   HQ++
Sbjct: 4   VGVEDLVKAGLSTEEAKYFQRVLEDIVNGAKGLDPSEVWREVVARRLLKPSHPHELHQLL 63

Query: 69  YYGCYKHY-----GPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQE 128
           Y+  Y ++     GP P  W P    +  TN+G+L+E+ G E LG  Y+DP +SFS F++
Sbjct: 64  YHSVYANWDVSTRGP-PLYWFPSLHQSKKTNLGRLMEKYGSEVLGPLYKDPITSFSLFRK 123

Query: 129 FSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL- 188
           FSV  PE YW  VL E+ ++F + P CIL   DS +   RS  G  LPG+V N A+ CL 
Sbjct: 124 FSVQQPEAYWSIVLKELSVSFQEAPKCIL---DSADKSKRS--GSLLPGSVLNIAECCLL 183

Query: 189 -SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDM 248
            +    +  D  AI+WRDEG D+  V  LTL+ELR +V LVA+AL+++ F KG AIAIDM
Sbjct: 184 PTSYPMKRDDSPAIVWRDEGYDDSEVNVLTLKELREKVMLVANALDAM-FSKGDAIAIDM 243

Query: 249 PMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLY 308
           PM VNAVVIYLAI+L+G VVVSIADSFAA+EI+TRL +S AKAIFTQD I+RG +   LY
Sbjct: 244 PMTVNAVVIYLAIILSGFVVVSIADSFAAKEIATRLRVSNAKAIFTQDFILRGGRKFSLY 303

Query: 309 SRIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKD-LKGVEFAAVEKSGESFTN 368
           SR++DA    AIV+P N +   ++LR+ DISW  F+  V +  +   +    +  +S  N
Sbjct: 304 SRVVDAAPHKAIVLPVNGSNLGLQLREQDISWEKFISNVSNQARSNHYTPFYQPVDSLIN 363

Query: 369 ILFSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLL 428
           ILFSSGTTGEPKAIPWT ++PL+ AAD W H+D + GDV  WPTNLGW+MGP L+++S L
Sbjct: 364 ILFSSGTTGEPKAIPWTQLSPLRCAADTWAHIDAQVGDVFCWPTNLGWVMGPVLLFSSFL 423

Query: 429 NRASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTG 488
           + A++ALY+GSPL  GF KFVQD+ VT+LG +PS+V++WK+T C  G DW+ I+ FASTG
Sbjct: 424 SGATLALYHGSPLGHGFGKFVQDSGVTILGTVPSLVKAWKNTQCMKGLDWTKIKVFASTG 483

Query: 489 EASSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILG 548
           EAS++D+ LWL  +A Y+PIIE CGGTE+   ++ GSL+Q QA  AFS  AM   L IL 
Sbjct: 484 EASNVDDDLWLSSQAYYQPIIECCGGTELASSYIQGSLVQPQAFGAFSTAAMTVGLVILD 543

Query: 549 NDDSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERS 608
            +  P P      GE+ L P   GA+  LLNAD+  VYFKGMP +NGM LRRHGD+ +R+
Sbjct: 544 ENGIPYPDKQECTGEVGLFPLYLGATDRLLNADNEKVYFKGMPLYNGMSLRRHGDILKRT 603

Query: 609 PRAYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTV 668
              ++  HGRADDTMNLGGIK SS+EIER+CN  DES++ET A+ V P  GGPEQLV+ V
Sbjct: 604 AGGFFIVHGRADDTMNLGGIKTSSIEIERVCNRADESVMETTAVSVAPVSGGPEQLVMFV 663

Query: 669 VFKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLA 721
           V K  S  + + ++LK  F+ A+Q NLNPLF+V  V      PRTA+NK++RRVLR Q+ 
Sbjct: 664 VLK--SGHNNEAERLKKKFSKAIQSNLNPLFKVSFVKIVEEFPRTASNKLLRRVLRDQMK 716

BLAST of MC06g0276 vs. ExPASy Swiss-Prot
Match: Q84P17 (Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE18 PE=2 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 3.5e-196
Identity = 350/729 (48.01%), Postives = 476/729 (65.29%), Query Frame = 0

Query: 3   YKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTP-ETWRH-ISKRVLSPDLPF 62
           +K++  ++  D    G+  E A++   L+S++I       P + W+  + + VL P  P 
Sbjct: 2   WKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHPH 61

Query: 63  SFHQMMYYGCYKH-----YGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSS 122
             HQ++YY  Y +     +GP P  W P    +  TN+G+L+E  G   LG +Y++P  S
Sbjct: 62  PLHQLLYYSVYSNWDSSVHGP-PLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 121

Query: 123 FSSFQEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNP 182
           F  F+ FSV +PEVYW  V+DE+ + F  PP CIL  +         P G WLP AV N 
Sbjct: 122 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS--------KPEGTWLPDAVLNI 181

Query: 183 AKDCL--SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGT 242
           A+ CL  S +  +  D VA++WR+EG D+ PV R+T++ELR +V LVA+A++   FEKG 
Sbjct: 182 AECCLMPSSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISG-SFEKGD 241

Query: 243 AIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGD 302
            IAIDMPM V+AV+IYLAI+LAG +VVSIADSFAA+EI+TRL ISKAK IFTQD I+RG 
Sbjct: 242 TIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGG 301

Query: 303 KSIPLYSRIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVE--FAAVEK 362
           +  PLYSR+++A     IV+P + T   ++LR+ D+SW  FL   K     E  +  +  
Sbjct: 302 RRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYL 361

Query: 363 SGESFTNILFSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPW 422
             ES  NILFSSGTTGEPKAIPWT ++P+++A D W H+D++ G    WPTNLGW+MGP 
Sbjct: 362 PVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPT 421

Query: 423 LVYASLLNRASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSI 482
           L+++  L  A++ALY+GSPL  GF KFVQDA VT+LG +PS+V++WK TNC  G +W+ I
Sbjct: 422 LMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKI 481

Query: 483 RCFASTGEASSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMG 542
           + FA+TGEAS++D+ LWL  +A YKP+IE CGGTE+   ++ GS LQ QA  AFS P+M 
Sbjct: 482 KFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMT 541

Query: 543 CSLFILGNDDSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRH 602
             + I   +  P P   P  GE+ L P   GA+  LLNA+H +VYFKGMP +    LRRH
Sbjct: 542 TRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRH 601

Query: 603 GDLFERSPRAYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGP 662
           GD+ +R+   YY   GRADDTMNLGGIK SS+EIER+C+  DE I ETAA+ + P  GGP
Sbjct: 602 GDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGP 661

Query: 663 EQLVVTVVFKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRR 721
           E LV+  V K         ++LK+ F+  +QK+LNPLF+V  V   P  PRTA++K++RR
Sbjct: 662 ELLVIFAVLKEGFKQQSG-EELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRR 719

BLAST of MC06g0276 vs. ExPASy Swiss-Prot
Match: Q4ZQG8 (Acetyl-coenzyme A synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) OX=205918 GN=acsA PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.8e-43
Identity = 178/629 (28.30%), Postives = 274/629 (43.56%), Query Frame = 0

Query: 114 SSFSSFQEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMN-DSPESQFRSPGGQWLPGAV 173
           +++ +  + SV NP+ +WR       + + KP + + Q + D      +     W     
Sbjct: 22  ATYKAMYQQSVVNPDGFWRE--QAQRLDWIKPFSVVKQTSFDDHRVDIK-----WFADGT 81

Query: 174 FNPAKDCLSINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKG 233
            N A +CL  +     D +AIIW  EG D    + +T REL AEV   A+AL      +G
Sbjct: 82  LNVAYNCLDRHLEERGDSIAIIW--EGDDPSEHREITYRELHAEVCKFANALRGQDVHRG 141

Query: 234 TAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRG 293
             + I MPM   AVV  LA    G +   +   F+   ++ R+    +K + T D  +RG
Sbjct: 142 DVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCSSKVVITADEGLRG 201

Query: 294 DKSIPLYSRIIDA-----QSPLAIVIPTNSTGFSMKL-RDSDISWHAFLERVKDLKGVEF 353
            K   L + +  A      S +  VI    TG  ++  R  DI +H+ LE    +     
Sbjct: 202 GKKTALKANVDRALTNPETSSVQKVIVCKRTGGEIEWNRHRDIWYHSLLE----VASSTC 261

Query: 354 AAVEKSGESFTNILFSSGTTGEPKAIPWTLVTPLKAAADAWCHM-DIRHGDVVAWPTNLG 413
           A  E   E    IL++SG+TG+PK +  T    L  AA     + D R G+V     ++G
Sbjct: 262 APKEMGAEESLFILYTSGSTGKPKGVLHTTAGYLLYAALTHERVFDYRPGEVYWCTADVG 321

Query: 414 WMMG-PWLVYASLLNRASMALYNGSPLSSGFV---KFVQDAKVTMLGVIPSIVRSW--KS 473
           W+ G  ++VY  L N A+  L+ G P         K +   KV +L   P+ +R+   + 
Sbjct: 322 WVTGHSYIVYGPLANGATTLLFEGVPNYPDITRVSKIIDKHKVNILYTAPTAIRAMMAEG 381

Query: 474 TNCTSGYDWSSIRCFASTGEASSIDEYLWLMGRACYK--PIIEYCGGTEIGGGFVTGSLL 533
           T    G D SS+R   S GE  + + + W       +  PI++    TE  GG +   L 
Sbjct: 382 TKSVEGADGSSLRLLGSVGEPINPEAWAWYYNTVGKQNCPIVDTWWQTET-GGILISPLP 441

Query: 534 QAQALAAFSA--PAMGCSLFILGNDDSPIPQSMPGIGELALGPFMFGASSSLLNADH--- 593
            A AL   SA  P  G    ++ N  + I  +    G L +     G S SL   DH   
Sbjct: 442 GATALKPGSATRPFFGVIPALVDNLGNLIEGAAE--GNLVILDSWPGQSRSLY-GDHDRF 501

Query: 594 YDVYFKGMPRWNGMILRRHGDLFERSPRAYYRAHGRADDTMNLGGIKVSSVEIERICNTV 653
            D YFK    + GM     GD   R    Y+   GR DD +N+ G ++ + EIE      
Sbjct: 502 VDTYFK---TFRGMYFT--GDGARRDEDGYFWITGRVDDVLNVSGHRMGTAEIES-AMVA 561

Query: 654 DESILETAAIGVPPHGGGPEQLVVTVVFKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVH 713
              + E A +GV PH    + + V V      + S   D L+    + ++K + P+    
Sbjct: 562 HPKVAEAAVVGV-PHDLKGQGIYVYVTLNGGEEPS---DALRTELRNWVRKEIGPIASPD 621

Query: 714 RVVPYPSLPRTATNKVMRRVLRQQLAIEH 722
            +   P LP+T + K+MRR+LR+    E+
Sbjct: 622 FIQWAPGLPKTRSGKIMRRILRKIATAEY 623

BLAST of MC06g0276 vs. ExPASy Swiss-Prot
Match: Q48G09 (Acetyl-coenzyme A synthetase OS=Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) OX=264730 GN=acsA PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 3.4e-42
Identity = 176/629 (27.98%), Postives = 274/629 (43.56%), Query Frame = 0

Query: 114 SSFSSFQEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMN-DSPESQFRSPGGQWLPGAV 173
           +++ +  + SV NP+ +WR       + + KP + + Q + D      +     W     
Sbjct: 22  ATYKAMYQQSVVNPDGFWRE--QAQRLDWIKPFSVVKQTSFDDHRVDIK-----WFADGT 81

Query: 174 FNPAKDCLSINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKG 233
            N A +CL  +     D +AIIW  EG +    + +T REL AEV   A+AL      +G
Sbjct: 82  LNVAYNCLDRHLAERGDTIAIIW--EGDNPSEHREITYRELHAEVCKFANALRGQDVHRG 141

Query: 234 TAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRG 293
             + I MPM   AVV  LA    G +   +   F+   ++ R+    +K + T D  +RG
Sbjct: 142 DVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCSSKVVITADEGLRG 201

Query: 294 DKSIPLYSRIIDA-----QSPLAIVIPTNSTGFSMKL-RDSDISWHAFLERVKDLKGVEF 353
            K   L + +  A      S +  VI    TG  ++  R  DI +H+ LE    +     
Sbjct: 202 GKKTALKANVDRALTNPETSSVQKVIVCKRTGGEIEWNRHRDIWYHSLLE----VASSTC 261

Query: 354 AAVEKSGESFTNILFSSGTTGEPKAIPWTLVTPLKAAADAWCHM-DIRHGDVVAWPTNLG 413
           A  E   E    IL++SG+TG+PK +  T    L  AA     + D + G++     ++G
Sbjct: 262 APKEMGAEESLFILYTSGSTGKPKGVLHTTAGYLLYAALTHERVFDYKPGEIYWCTADVG 321

Query: 414 WMMG-PWLVYASLLNRASMALYNGSPLSSGFV---KFVQDAKVTMLGVIPSIVRSW--KS 473
           W+ G  ++VY  L N A+  L+ G P         K +   KV +L   P+ +R+   + 
Sbjct: 322 WVTGHSYIVYGPLANGATTLLFEGVPNYPDITRVSKIIDKHKVNILYTAPTAIRAMMAEG 381

Query: 474 TNCTSGYDWSSIRCFASTGEASSIDEYLWLMGRACYK--PIIEYCGGTEIGGGFVTGSLL 533
           T    G D SS+R   S GE  + + + W       +  PI++    TE  GG +   L 
Sbjct: 382 TKSVEGADGSSLRLLGSVGEPINPEAWGWYYNTVGKQNCPIVDTWWQTET-GGILISPLP 441

Query: 534 QAQALAAFSA--PAMGCSLFILGNDDSPIPQSMPGIGELALGPFMFGASSSLLNADH--- 593
            A AL   SA  P  G    ++ N  + I  +    G L +     G S SL   DH   
Sbjct: 442 GATALKPGSATRPFFGVIPALVDNLGNLIEGAAE--GNLVILDSWPGQSRSLY-GDHDRF 501

Query: 594 YDVYFKGMPRWNGMILRRHGDLFERSPRAYYRAHGRADDTMNLGGIKVSSVEIERICNTV 653
            D YFK    + GM     GD   R    YY   GR DD +N+ G ++ + EIE      
Sbjct: 502 VDTYFK---TFRGMYFT--GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIES-AMVA 561

Query: 654 DESILETAAIGVPPHGGGPEQLVVTVVFKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVH 713
              + E A +GV PH    + + V V      + S   D L+    + ++K + P+    
Sbjct: 562 HPKVAEAAVVGV-PHDLKGQGIYVYVTLNGGEEPS---DALRTELRNWVRKEIGPIASPD 621

Query: 714 RVVPYPSLPRTATNKVMRRVLRQQLAIEH 722
            +   P LP+T + K+MRR+LR+    E+
Sbjct: 622 FIQWAPGLPKTRSGKIMRRILRKIATAEY 623

BLAST of MC06g0276 vs. NCBI nr
Match: XP_022135245.1 (probable acyl-activating enzyme 17, peroxisomal [Momordica charantia])

HSP 1 Score: 1465 bits (3793), Expect = 0.0
Identity = 716/725 (98.76%), Postives = 723/725 (99.72%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           M+YK LDSIT I+IEALG+ASEVAEKLHGLLSEIIRS+GNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MEYKALDSITAIEIEALGIASEVAEKLHGLLSEIIRSYGNGTPETWRHISKRVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGC+KHYGPDPPAW+PEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ
Sbjct: 61  SFHQMMYYGCFKHYGPDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEF+AVEKSGESFTNIL
Sbjct: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFSAVEKSGESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND
Sbjct: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
           DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR
Sbjct: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
           AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF
Sbjct: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE
Sbjct: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. NCBI nr
Match: XP_022135246.1 (probable acyl-activating enzyme 17, peroxisomal [Momordica charantia])

HSP 1 Score: 1377 bits (3563), Expect = 0.0
Identity = 661/725 (91.17%), Postives = 696/725 (96.00%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLL+RRGKEFLGSNYRDP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLKRRGKEFLGSNYRDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRT+LDEMHITFSKPP+CILQMNDS ESQF SPGGQWLPGAVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTMLDEMHITFSKPPHCILQMNDSTESQFSSPGGQWLPGAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEGCDNLPVKRLTL ELR +VWL+AHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDNLPVKRLTLGELRTDVWLIAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYL IVLAGHVVVSIADSF+AREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLGIVLAGHVVVSIADSFSAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQSP+AIVIP+ STGFS KLRD DISWHAFLERV+DL+GVEFAAVE++ ESFTNIL
Sbjct: 301 RVVDAQSPMAIVIPSRSTGFSRKLRDEDISWHAFLERVEDLRGVEFAAVEQAAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWC+MDI  GDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCYMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPL SGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLGSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           S++DE LWLMGRACYKP+IE CGGTEIGGGF+TGSLLQ QALAAFS PAMGCSLFILGND
Sbjct: 481 SNVDENLWLMGRACYKPVIEICGGTEIGGGFITGSLLQPQALAAFSTPAMGCSLFILGND 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
             PIPQ+MPGIGELALGPF+FGASS+LLNADHYD+YFKGMP WNGM+LRRHGD+FERSPR
Sbjct: 541 GFPIPQNMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPHWNGMVLRRHGDVFERSPR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            YYRAHGRADD MNLGGIKVSSVEIERICNT+D+SILETAAIGVPP GGGPEQLV+ VV 
Sbjct: 601 GYYRAHGRADDAMNLGGIKVSSVEIERICNTIDDSILETAAIGVPPLGGGPEQLVIAVVL 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNP +TSPDLDKLKL FNS+LQKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQLA+E
Sbjct: 661 KNPGETSPDLDKLKLCFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLAVE 720

Query: 721 HKTKL 725
            +TKL
Sbjct: 721 RRTKL 725

BLAST of MC06g0276 vs. NCBI nr
Match: XP_011648674.1 (probable acyl-activating enzyme 17, peroxisomal [Cucumis sativus] >KGN63781.1 hypothetical protein Csa_013289 [Cucumis sativus])

HSP 1 Score: 1362 bits (3524), Expect = 0.0
Identity = 653/725 (90.07%), Postives = 699/725 (96.41%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYK LDSITVIDIEALGV S++A++LHG L EIIR++GNGTP TW HISK++L+PDLPF
Sbjct: 1   MDYKPLDSITVIDIEALGVGSQLAKQLHGSLGEIIRTYGNGTPATWLHISKKLLTPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYK++GPDPPAWIPEPENAVFTNVG+LLE+RGKEF+GS+Y DP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKNFGPDPPAWIPEPENAVFTNVGKLLEKRGKEFMGSSYSDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEM+ITFSKPPNCILQ+NDSPES+F SPGGQWLPGAVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKFSSPGGQWLPGAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEG D+LP+KRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGYDDLPLKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQ+PLAIVIPTNSTGFSMKLRD DISWHAFL++VKDLKGVEF AVE+S ESFTNIL
Sbjct: 301 RVVDAQAPLAIVIPTNSTGFSMKLRDGDISWHAFLDKVKDLKGVEFDAVEQSAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN+
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKF+QDAK TMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFIQDAKTTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SS+DEYLWLMGRACYKPIIEYCGGTEIGGGFVTGS+LQAQALAAFS PAMGCSLFILG+D
Sbjct: 481 SSVDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSMLQAQALAAFSTPAMGCSLFILGDD 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
            SPIPQ+MPGIGELALGPFMFGASSSLLNADHYD+YFKGMP WNGM++RRHGDLFERSPR
Sbjct: 541 GSPIPQNMPGIGELALGPFMFGASSSLLNADHYDIYFKGMPIWNGMLMRRHGDLFERSPR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            +YRAHGRADDTMNLGGIKVSSVEIERICNTV+ SILETAAIGVPP GGGPE+LV+ +VF
Sbjct: 601 GFYRAHGRADDTMNLGGIKVSSVEIERICNTVNNSILETAAIGVPPQGGGPERLVIAIVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPS+TS DLDKLK SFNSA+QKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQL +E
Sbjct: 661 KNPSETSIDLDKLKQSFNSAIQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLTLE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. NCBI nr
Match: TYK28383.1 (putative acyl-activating enzyme 17, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1358 bits (3514), Expect = 0.0
Identity = 653/725 (90.07%), Postives = 694/725 (95.72%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYK LDSITVIDIEALG+ SEVA++LHG L EII S+GNGTPETW HISK VLSPDLPF
Sbjct: 1   MDYKALDSITVIDIEALGIDSEVAKQLHGSLGEIICSYGNGTPETWHHISKEVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYK++GPDPPAWIPEPENA+FTNVG+LLE+RGKEF+GS+Y DP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKYFGPDPPAWIPEPENALFTNVGKLLEKRGKEFMGSSYSDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEM+ITFSKPPNCILQ+NDSPES+  SPGGQWLP AVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKISSPGGQWLPRAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEGCD+LP+KRLTLRELR+EVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDDLPLKRLTLRELRSEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQ+PLAIVIPTNSTGFSMKLRD DISWHAFL+ VKDLKGVEF AVE+S ESFTNIL
Sbjct: 301 RVVDAQAPLAIVIPTNSTGFSMKLRDGDISWHAFLDEVKDLKGVEFDAVEQSAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN+
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKF+QDAK TMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFIQDAKTTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SS+DEYLWLMGRACYKPIIEYCGGTEIGGGFVTGS+LQAQALAAFS PAMGCSLFILG+D
Sbjct: 481 SSVDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSMLQAQALAAFSTPAMGCSLFILGDD 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
            SPIPQ+MPGIGEL LGP+MFGASSSLLNADHYD+YFKGMP WNGM++RRHGDLFERS R
Sbjct: 541 GSPIPQNMPGIGELVLGPYMFGASSSLLNADHYDIYFKGMPIWNGMLMRRHGDLFERSLR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            +YRAHGRADDTMNLGGIKVSSVEIERICNTVD SILETAAIGVPP GGGPE+LV+ VVF
Sbjct: 601 GFYRAHGRADDTMNLGGIKVSSVEIERICNTVDNSILETAAIGVPPQGGGPERLVIAVVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPS+TS DLDKLK SFNSA+QKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQL +E
Sbjct: 661 KNPSETSLDLDKLKQSFNSAIQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLTLE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. NCBI nr
Match: XP_008453341.1 (PREDICTED: probable acyl-activating enzyme 17, peroxisomal [Cucumis melo])

HSP 1 Score: 1356 bits (3509), Expect = 0.0
Identity = 652/725 (89.93%), Postives = 694/725 (95.72%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYK LDSITVIDIEALG+ SEVA++LHG L EII S+GNGTPETW HISK VLSPDLPF
Sbjct: 1   MDYKALDSITVIDIEALGIDSEVAKQLHGSLGEIICSYGNGTPETWHHISKEVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYK++GPDPPAWIPEPENA+FTNVG+LLE+RGKEF+GS+Y DP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKNFGPDPPAWIPEPENALFTNVGKLLEKRGKEFMGSSYSDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEM+ITFSKPPNCILQ+NDSPES+  SPGGQWLP AVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKISSPGGQWLPRAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEGCD+LP+KRLTLRELR+EVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDDLPLKRLTLRELRSEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQ+PLAIVIPTNSTGFSMKLRD DISWHAFL+ VKDLKGVEF AVE+S ESFTNIL
Sbjct: 301 RVVDAQAPLAIVIPTNSTGFSMKLRDGDISWHAFLDEVKDLKGVEFDAVEQSAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN+
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKF+QDAK TMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFIQDAKTTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SS+DEYLWLMGRACYKPIIEYCGGTEIGGGFVTGS+LQAQALAAFS PAMGCSLFILG+D
Sbjct: 481 SSVDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSMLQAQALAAFSTPAMGCSLFILGDD 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
            SPIPQ+MPGIGEL LGP+MFGASSSLLNADHYD+YFKGMP WNGM++RRHGDLFERS R
Sbjct: 541 GSPIPQNMPGIGELVLGPYMFGASSSLLNADHYDIYFKGMPIWNGMLMRRHGDLFERSLR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            +YRAHGRADDTMNLGGIKVSSVEIERICNTVD SILETAAIGVPP GGGPE+LV+ VVF
Sbjct: 601 GFYRAHGRADDTMNLGGIKVSSVEIERICNTVDNSILETAAIGVPPQGGGPERLVIAVVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPS+TS DLD+LK SFNSA+QKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQL +E
Sbjct: 661 KNPSETSLDLDQLKQSFNSAIQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLTLE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. ExPASy TrEMBL
Match: A0A6J1C0M0 (probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111007255 PE=4 SV=1)

HSP 1 Score: 1465 bits (3793), Expect = 0.0
Identity = 716/725 (98.76%), Postives = 723/725 (99.72%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           M+YK LDSIT I+IEALG+ASEVAEKLHGLLSEIIRS+GNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MEYKALDSITAIEIEALGIASEVAEKLHGLLSEIIRSYGNGTPETWRHISKRVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGC+KHYGPDPPAW+PEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ
Sbjct: 61  SFHQMMYYGCFKHYGPDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEF+AVEKSGESFTNIL
Sbjct: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFSAVEKSGESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND
Sbjct: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
           DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR
Sbjct: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
           AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF
Sbjct: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE
Sbjct: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. ExPASy TrEMBL
Match: A0A6J1C048 (probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111007256 PE=4 SV=1)

HSP 1 Score: 1377 bits (3563), Expect = 0.0
Identity = 661/725 (91.17%), Postives = 696/725 (96.00%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLL+RRGKEFLGSNYRDP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLKRRGKEFLGSNYRDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRT+LDEMHITFSKPP+CILQMNDS ESQF SPGGQWLPGAVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTMLDEMHITFSKPPHCILQMNDSTESQFSSPGGQWLPGAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEGCDNLPVKRLTL ELR +VWL+AHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDNLPVKRLTLGELRTDVWLIAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYL IVLAGHVVVSIADSF+AREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLGIVLAGHVVVSIADSFSAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQSP+AIVIP+ STGFS KLRD DISWHAFLERV+DL+GVEFAAVE++ ESFTNIL
Sbjct: 301 RVVDAQSPMAIVIPSRSTGFSRKLRDEDISWHAFLERVEDLRGVEFAAVEQAAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWC+MDI  GDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCYMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPL SGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLGSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           S++DE LWLMGRACYKP+IE CGGTEIGGGF+TGSLLQ QALAAFS PAMGCSLFILGND
Sbjct: 481 SNVDENLWLMGRACYKPVIEICGGTEIGGGFITGSLLQPQALAAFSTPAMGCSLFILGND 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
             PIPQ+MPGIGELALGPF+FGASS+LLNADHYD+YFKGMP WNGM+LRRHGD+FERSPR
Sbjct: 541 GFPIPQNMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPHWNGMVLRRHGDVFERSPR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            YYRAHGRADD MNLGGIKVSSVEIERICNT+D+SILETAAIGVPP GGGPEQLV+ VV 
Sbjct: 601 GYYRAHGRADDAMNLGGIKVSSVEIERICNTIDDSILETAAIGVPPLGGGPEQLVIAVVL 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNP +TSPDLDKLKL FNS+LQKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQLA+E
Sbjct: 661 KNPGETSPDLDKLKLCFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLAVE 720

Query: 721 HKTKL 725
            +TKL
Sbjct: 721 RRTKL 725

BLAST of MC06g0276 vs. ExPASy TrEMBL
Match: A0A0A0LUS2 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G015740 PE=4 SV=1)

HSP 1 Score: 1362 bits (3524), Expect = 0.0
Identity = 653/725 (90.07%), Postives = 699/725 (96.41%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYK LDSITVIDIEALGV S++A++LHG L EIIR++GNGTP TW HISK++L+PDLPF
Sbjct: 1   MDYKPLDSITVIDIEALGVGSQLAKQLHGSLGEIIRTYGNGTPATWLHISKKLLTPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYK++GPDPPAWIPEPENAVFTNVG+LLE+RGKEF+GS+Y DP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKNFGPDPPAWIPEPENAVFTNVGKLLEKRGKEFMGSSYSDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEM+ITFSKPPNCILQ+NDSPES+F SPGGQWLPGAVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKFSSPGGQWLPGAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEG D+LP+KRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGYDDLPLKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQ+PLAIVIPTNSTGFSMKLRD DISWHAFL++VKDLKGVEF AVE+S ESFTNIL
Sbjct: 301 RVVDAQAPLAIVIPTNSTGFSMKLRDGDISWHAFLDKVKDLKGVEFDAVEQSAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN+
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKF+QDAK TMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFIQDAKTTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SS+DEYLWLMGRACYKPIIEYCGGTEIGGGFVTGS+LQAQALAAFS PAMGCSLFILG+D
Sbjct: 481 SSVDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSMLQAQALAAFSTPAMGCSLFILGDD 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
            SPIPQ+MPGIGELALGPFMFGASSSLLNADHYD+YFKGMP WNGM++RRHGDLFERSPR
Sbjct: 541 GSPIPQNMPGIGELALGPFMFGASSSLLNADHYDIYFKGMPIWNGMLMRRHGDLFERSPR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            +YRAHGRADDTMNLGGIKVSSVEIERICNTV+ SILETAAIGVPP GGGPE+LV+ +VF
Sbjct: 601 GFYRAHGRADDTMNLGGIKVSSVEIERICNTVNNSILETAAIGVPPQGGGPERLVIAIVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPS+TS DLDKLK SFNSA+QKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQL +E
Sbjct: 661 KNPSETSIDLDKLKQSFNSAIQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLTLE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. ExPASy TrEMBL
Match: A0A5D3DXF5 (Putative acyl-activating enzyme 17, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00090 PE=4 SV=1)

HSP 1 Score: 1358 bits (3514), Expect = 0.0
Identity = 653/725 (90.07%), Postives = 694/725 (95.72%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYK LDSITVIDIEALG+ SEVA++LHG L EII S+GNGTPETW HISK VLSPDLPF
Sbjct: 1   MDYKALDSITVIDIEALGIDSEVAKQLHGSLGEIICSYGNGTPETWHHISKEVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYK++GPDPPAWIPEPENA+FTNVG+LLE+RGKEF+GS+Y DP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKYFGPDPPAWIPEPENALFTNVGKLLEKRGKEFMGSSYSDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEM+ITFSKPPNCILQ+NDSPES+  SPGGQWLP AVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKISSPGGQWLPRAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEGCD+LP+KRLTLRELR+EVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDDLPLKRLTLRELRSEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQ+PLAIVIPTNSTGFSMKLRD DISWHAFL+ VKDLKGVEF AVE+S ESFTNIL
Sbjct: 301 RVVDAQAPLAIVIPTNSTGFSMKLRDGDISWHAFLDEVKDLKGVEFDAVEQSAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN+
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKF+QDAK TMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFIQDAKTTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SS+DEYLWLMGRACYKPIIEYCGGTEIGGGFVTGS+LQAQALAAFS PAMGCSLFILG+D
Sbjct: 481 SSVDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSMLQAQALAAFSTPAMGCSLFILGDD 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
            SPIPQ+MPGIGEL LGP+MFGASSSLLNADHYD+YFKGMP WNGM++RRHGDLFERS R
Sbjct: 541 GSPIPQNMPGIGELVLGPYMFGASSSLLNADHYDIYFKGMPIWNGMLMRRHGDLFERSLR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            +YRAHGRADDTMNLGGIKVSSVEIERICNTVD SILETAAIGVPP GGGPE+LV+ VVF
Sbjct: 601 GFYRAHGRADDTMNLGGIKVSSVEIERICNTVDNSILETAAIGVPPQGGGPERLVIAVVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPS+TS DLDKLK SFNSA+QKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQL +E
Sbjct: 661 KNPSETSLDLDKLKQSFNSAIQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLTLE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. ExPASy TrEMBL
Match: A0A1S3BW17 (probable acyl-activating enzyme 17, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103494091 PE=4 SV=1)

HSP 1 Score: 1356 bits (3509), Expect = 0.0
Identity = 652/725 (89.93%), Postives = 694/725 (95.72%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60
           MDYK LDSITVIDIEALG+ SEVA++LHG L EII S+GNGTPETW HISK VLSPDLPF
Sbjct: 1   MDYKALDSITVIDIEALGIDSEVAKQLHGSLGEIICSYGNGTPETWHHISKEVLSPDLPF 60

Query: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120
           SFHQMMYYGCYK++GPDPPAWIPEPENA+FTNVG+LLE+RGKEF+GS+Y DP SSFSSFQ
Sbjct: 61  SFHQMMYYGCYKNFGPDPPAWIPEPENALFTNVGKLLEKRGKEFMGSSYSDPLSSFSSFQ 120

Query: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180
           EFSVSNPEVYWRTVLDEM+ITFSKPPNCILQ+NDSPES+  SPGGQWLP AVFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKISSPGGQWLPRAVFNPAKDCL 180

Query: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240
           S+NENRSLDDVAIIWRDEGCD+LP+KRLTLRELR+EVWLVAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDDLPLKRLTLRELRSEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRL+ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNIL 360
           R++DAQ+PLAIVIPTNSTGFSMKLRD DISWHAFL+ VKDLKGVEF AVE+S ESFTNIL
Sbjct: 301 RVVDAQAPLAIVIPTNSTGFSMKLRDGDISWHAFLDEVKDLKGVEFDAVEQSAESFTNIL 360

Query: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420
           FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN+
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480
           ASMALYNGSPLSSGFVKF+QDAK TMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFIQDAKTTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540
           SS+DEYLWLMGRACYKPIIEYCGGTEIGGGFVTGS+LQAQALAAFS PAMGCSLFILG+D
Sbjct: 481 SSVDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSMLQAQALAAFSTPAMGCSLFILGDD 540

Query: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600
            SPIPQ+MPGIGEL LGP+MFGASSSLLNADHYD+YFKGMP WNGM++RRHGDLFERS R
Sbjct: 541 GSPIPQNMPGIGELVLGPYMFGASSSLLNADHYDIYFKGMPIWNGMLMRRHGDLFERSLR 600

Query: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660
            +YRAHGRADDTMNLGGIKVSSVEIERICNTVD SILETAAIGVPP GGGPE+LV+ VVF
Sbjct: 601 GFYRAHGRADDTMNLGGIKVSSVEIERICNTVDNSILETAAIGVPPQGGGPERLVIAVVF 660

Query: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720
           KNPS+TS DLD+LK SFNSA+QKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQL +E
Sbjct: 661 KNPSETSLDLDQLKQSFNSAIQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLTLE 720

Query: 721 HKTKL 725
           HKTKL
Sbjct: 721 HKTKL 725

BLAST of MC06g0276 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 1048.5 bits (2710), Expect = 2.5e-306
Identity = 503/718 (70.06%), Postives = 602/718 (83.84%), Query Frame = 0

Query: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGT-PETWRHISKRVLSPDLP 60
           M YK+L+SIT  DIEALG++ +V+EKL   L +II  HG+ T PETW  IS+R+L P+LP
Sbjct: 1   MAYKSLNSITKSDIEALGISGDVSEKLLRDLEDII--HGSSTPPETWIQISRRILHPNLP 60

Query: 61  FSFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSF 120
           FSFHQMMYYGCYK +GPD PAWIP+P+ A  TNVG+LLE+RGKEFLG NY++P SSFSSF
Sbjct: 61  FSFHQMMYYGCYKDFGPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSFSSF 120

Query: 121 QEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDC 180
           QEFSVSNPEVYW+TVLDE++I FS PP CIL+ + S +    +PGG+WLPGA  NPA++C
Sbjct: 121 QEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGD----NPGGKWLPGAYLNPARNC 180

Query: 181 LSINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDM 240
           L+    R LDD+ I WRDEG D+LPV  +TL ELR++VWL AHAL+++G E+ +AIA+DM
Sbjct: 181 LTNGFKRRLDDIVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVDM 240

Query: 241 PMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLY 300
           PMNV +V+IYLAIVLAGHVVVSIADSF+ REISTRL ISKAKAIFTQD+IIRGDKSIPLY
Sbjct: 241 PMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPLY 300

Query: 301 SRIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFAAVEKSGESFTNI 360
            R++DA++PLAIV+P   +   MKLR+ D+SW+ FL   ++L+GVE+ A EK   ++TNI
Sbjct: 301 RRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNLRGVEYVAAEKPAGAYTNI 360

Query: 361 LFSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLN 420
           LFSSGTTGEPKAIPWT ++PLK+AADAWCH+D++ GDVVAWPTNLGWMMGPWLVYASL+N
Sbjct: 361 LFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASLIN 420

Query: 421 RASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGE 480
            A M LYNGSPL   F KFVQDA+V++LGVIPSIVR+W+++N TSGYDWS IRCF STGE
Sbjct: 421 GACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGSTGE 480

Query: 481 ASSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGN 540
           AS+IDEYLWLMGRA YKP+IEYCGGTEIGG F++GSLLQ Q+LAAFS  AMGC LFIL  
Sbjct: 481 ASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFILDE 540

Query: 541 DDSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSP 600
           D +PIP    G+GELAL P MFGASS+LLN +H+ VYF+GMP + G ILRRHGDLFER+ 
Sbjct: 541 DSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFERTS 600

Query: 601 RAYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVV 660
           + YYRAHGRADDTMNLGGIKV S+EIER+CN+VD+S+LETAAIGVPP  GGPEQLV+ VV
Sbjct: 601 KGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVV 660

Query: 661 FKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQL 718
           FK+P  ++PDL+ LK SFNS +QK LNPLF+V  VV  PSLPRTATNKVMRRVLRQQL
Sbjct: 661 FKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL 712

BLAST of MC06g0276 vs. TAIR 10
Match: AT1G55320.1 (acyl-activating enzyme 18 )

HSP 1 Score: 686.4 bits (1770), Expect = 2.5e-197
Identity = 350/729 (48.01%), Postives = 476/729 (65.29%), Query Frame = 0

Query: 3   YKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTP-ETWRH-ISKRVLSPDLPF 62
           +K++  ++  D    G+  E A++   L+S++I       P + W+  + + VL P  P 
Sbjct: 2   WKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHPH 61

Query: 63  SFHQMMYYGCYKH-----YGPDPPAWIPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSS 122
             HQ++YY  Y +     +GP P  W P    +  TN+G+L+E  G   LG +Y++P  S
Sbjct: 62  PLHQLLYYSVYSNWDSSVHGP-PLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 121

Query: 123 FSSFQEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNP 182
           F  F+ FSV +PEVYW  V+DE+ + F  PP CIL  +         P G WLP AV N 
Sbjct: 122 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS--------KPEGTWLPDAVLNI 181

Query: 183 AKDCL--SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGT 242
           A+ CL  S +  +  D VA++WR+EG D+ PV R+T++ELR +V LVA+A++   FEKG 
Sbjct: 182 AECCLMPSSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISG-SFEKGD 241

Query: 243 AIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGD 302
            IAIDMPM V+AV+IYLAI+LAG +VVSIADSFAA+EI+TRL ISKAK IFTQD I+RG 
Sbjct: 242 TIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGG 301

Query: 303 KSIPLYSRIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVE--FAAVEK 362
           +  PLYSR+++A     IV+P + T   ++LR+ D+SW  FL   K     E  +  +  
Sbjct: 302 RRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYL 361

Query: 363 SGESFTNILFSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPW 422
             ES  NILFSSGTTGEPKAIPWT ++P+++A D W H+D++ G    WPTNLGW+MGP 
Sbjct: 362 PVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPT 421

Query: 423 LVYASLLNRASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSI 482
           L+++  L  A++ALY+GSPL  GF KFVQDA VT+LG +PS+V++WK TNC  G +W+ I
Sbjct: 422 LMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKI 481

Query: 483 RCFASTGEASSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMG 542
           + FA+TGEAS++D+ LWL  +A YKP+IE CGGTE+   ++ GS LQ QA  AFS P+M 
Sbjct: 482 KFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMT 541

Query: 543 CSLFILGNDDSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRH 602
             + I   +  P P   P  GE+ L P   GA+  LLNA+H +VYFKGMP +    LRRH
Sbjct: 542 TRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRH 601

Query: 603 GDLFERSPRAYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGP 662
           GD+ +R+   YY   GRADDTMNLGGIK SS+EIER+C+  DE I ETAA+ + P  GGP
Sbjct: 602 GDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGP 661

Query: 663 EQLVVTVVFKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRR 721
           E LV+  V K         ++LK+ F+  +QK+LNPLF+V  V   P  PRTA++K++RR
Sbjct: 662 ELLVIFAVLKEGFKQQSG-EELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRR 719

BLAST of MC06g0276 vs. TAIR 10
Match: AT1G55320.2 (acyl-activating enzyme 18 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.8e-152
Identity = 268/504 (53.17%), Postives = 351/504 (69.64%), Query Frame = 0

Query: 219 LVAHALNSIGFEKGTAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNIS 278
           LVA+A++   FEKG  IAIDMPM V+AV+IYLAI+LAG +VVSIADSFAA+EI+TRL IS
Sbjct: 2   LVANAISG-SFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKIS 61

Query: 279 KAKAIFTQDLIIRGDKSIPLYSRIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERV 338
           KAK IFTQD I+RG +  PLYSR+++A     IV+P + T   ++LR+ D+SW  FL   
Sbjct: 62  KAKGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNA 121

Query: 339 KDLKGVE--FAAVEKSGESFTNILFSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGD 398
           K     E  +  +    ES  NILFSSGTTGEPKAIPWT ++P+++A D W H+D++ G 
Sbjct: 122 KPHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGH 181

Query: 399 VVAWPTNLGWMMGPWLVYASLLNRASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRS 458
              WPTNLGW+MGP L+++  L  A++ALY+GSPL  GF KFVQDA VT+LG +PS+V++
Sbjct: 182 TYCWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKT 241

Query: 459 WKSTNCTSGYDWSSIRCFASTGEASSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSL 518
           WK TNC  G +W+ I+ FA+TGEAS++D+ LWL  +A YKP+IE CGGTE+   ++ GS 
Sbjct: 242 WKRTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSP 301

Query: 519 LQAQALAAFSAPAMGCSLFILGNDDSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVY 578
           LQ QA  AFS P+M   + I   +  P P   P  GE+ L P   GA+  LLNA+H +VY
Sbjct: 302 LQPQAFGAFSTPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVY 361

Query: 579 FKGMPRWNGMILRRHGDLFERSPRAYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESI 638
           FKGMP +    LRRHGD+ +R+   YY   GRADDTMNLGGIK SS+EIER+C+  DE I
Sbjct: 362 FKGMPMYKETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECI 421

Query: 639 LETAAIGVPPHGGGPEQLVVTVVFKNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVP 698
            ETAA+ + P  GGPE LV+  V K         ++LK+ F+  +QK+LNPLF+V  V  
Sbjct: 422 SETAAVTLTPPNGGPELLVIFAVLKEGFKQQSG-EELKMKFSRTIQKDLNPLFKVSFVKI 481

Query: 699 YPSLPRTATNKVMRRVLRQQLAIE 721
            P  PRTA++K++RRVLR Q+  E
Sbjct: 482 VPEFPRTASSKLLRRVLRDQIKQE 503

BLAST of MC06g0276 vs. TAIR 10
Match: AT5G36880.1 (acetyl-CoA synthetase )

HSP 1 Score: 119.0 bits (297), Expect = 1.6e-26
Identity = 152/644 (23.60%), Postives = 253/644 (39.29%), Query Frame = 0

Query: 102 KEFLGSNYRDPFSSFSSFQEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFR 161
           KEF G         +    + S+ +P  +W  +  E +           +  D  +    
Sbjct: 46  KEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPIS 105

Query: 162 SPGGQWLPGAVFNPAKDCLSINENRSL-DDVAIIWRDEGCDNLPVKRLTLRELRAEVWLV 221
               +W  G + N   +CL  N    L D  AI W  EG +      LT  EL   V  +
Sbjct: 106 I---EWFKGGITNICYNCLDKNVEAGLGDKTAIHW--EGNELGVDASLTYSELLQRVCQL 165

Query: 222 AHALNSIGFEKGTAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKA 281
           A+ L   G +KG A+ I +PM +   +  LA    G V   +   F+A  ++ R+   K 
Sbjct: 166 ANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKP 225

Query: 282 KAIFTQDLIIRGDKSIPLYSRIIDA------QSPLAIVIPTNSTGFSMKLRDS------- 341
             I T + + RG K+I L   I+DA      +  +++ I           R++       
Sbjct: 226 NVILTCNAVKRGPKTINL-KAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGR 285

Query: 342 DISWHAFLERVKDLKGVEFAAVEKSGESFTNILFSSGTTGEPKAIPWTLVTPLKAAADAW 401
           D+ W   + +      VE+       E    +L++SG+TG+PK +  T    +   A  +
Sbjct: 286 DVWWQDVISQYPTSCEVEWV----DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTF 345

Query: 402 CH-MDIRHGDVVAWPTNLGWMMG-PWLVYASLLNRASMALYNGSPL---SSGFVKFVQDA 461
            +  D +  DV     + GW+ G  ++ Y  +LN A++ ++ G+P           V   
Sbjct: 346 KYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKY 405

Query: 462 KVTMLGVIPSIVRSW--KSTNCTSGYDWSSIRCFASTGEASSIDEYLWLMG----RACYK 521
           KV++    P++VRS         + +   S+R   S GE  +   + W         C  
Sbjct: 406 KVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC-- 465

Query: 522 PIIEYCGGTEIGGGFVT---GSLLQAQALAAFSAPAMGCSLFILGNDDSPIPQSMPGIGE 581
           PI +    TE GG  +T   G+  Q    A F  P  G    I+    + I     G   
Sbjct: 466 PISDTWWQTETGGFMITPLPGAWPQKPGSATF--PFFGVQPVIVDEKGNEIEGECSGY-- 525

Query: 582 LALGPFMFGASSSLL-NADHYD-VYFKGMPRWNGMILRRHGDLFERSPRAYYRAHGRADD 641
           L +     GA  +L  + + Y+  YFK    +        GD   R    YY   GR DD
Sbjct: 526 LCVKGSWPGAFRTLFGDHERYETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDD 585

Query: 642 TMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVFKNPSDTSPDLD 701
            +N+ G ++ + E+E     +     E A +G+     G         F    +  P  +
Sbjct: 586 VINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKGQG----IYAFVTLLEGVPYSE 645

Query: 702 KLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQ 716
           +L+ S    ++  +       R+   P LP+T + K+MRR+LR+
Sbjct: 646 ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 663

BLAST of MC06g0276 vs. TAIR 10
Match: AT5G36880.2 (acetyl-CoA synthetase )

HSP 1 Score: 119.0 bits (297), Expect = 1.6e-26
Identity = 152/644 (23.60%), Postives = 253/644 (39.29%), Query Frame = 0

Query: 102 KEFLGSNYRDPFSSFSSFQEFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFR 161
           KEF G         +    + S+ +P  +W  +  E +           +  D  +    
Sbjct: 96  KEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPIS 155

Query: 162 SPGGQWLPGAVFNPAKDCLSINENRSL-DDVAIIWRDEGCDNLPVKRLTLRELRAEVWLV 221
               +W  G + N   +CL  N    L D  AI W  EG +      LT  EL   V  +
Sbjct: 156 I---EWFKGGITNICYNCLDKNVEAGLGDKTAIHW--EGNELGVDASLTYSELLQRVCQL 215

Query: 222 AHALNSIGFEKGTAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKA 281
           A+ L   G +KG A+ I +PM +   +  LA    G V   +   F+A  ++ R+   K 
Sbjct: 216 ANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKP 275

Query: 282 KAIFTQDLIIRGDKSIPLYSRIIDA------QSPLAIVIPTNSTGFSMKLRDS------- 341
             I T + + RG K+I L   I+DA      +  +++ I           R++       
Sbjct: 276 NVILTCNAVKRGPKTINL-KAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGR 335

Query: 342 DISWHAFLERVKDLKGVEFAAVEKSGESFTNILFSSGTTGEPKAIPWTLVTPLKAAADAW 401
           D+ W   + +      VE+       E    +L++SG+TG+PK +  T    +   A  +
Sbjct: 336 DVWWQDVISQYPTSCEVEWV----DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTF 395

Query: 402 CH-MDIRHGDVVAWPTNLGWMMG-PWLVYASLLNRASMALYNGSPL---SSGFVKFVQDA 461
            +  D +  DV     + GW+ G  ++ Y  +LN A++ ++ G+P           V   
Sbjct: 396 KYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKY 455

Query: 462 KVTMLGVIPSIVRSW--KSTNCTSGYDWSSIRCFASTGEASSIDEYLWLMG----RACYK 521
           KV++    P++VRS         + +   S+R   S GE  +   + W         C  
Sbjct: 456 KVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC-- 515

Query: 522 PIIEYCGGTEIGGGFVT---GSLLQAQALAAFSAPAMGCSLFILGNDDSPIPQSMPGIGE 581
           PI +    TE GG  +T   G+  Q    A F  P  G    I+    + I     G   
Sbjct: 516 PISDTWWQTETGGFMITPLPGAWPQKPGSATF--PFFGVQPVIVDEKGNEIEGECSGY-- 575

Query: 582 LALGPFMFGASSSLL-NADHYD-VYFKGMPRWNGMILRRHGDLFERSPRAYYRAHGRADD 641
           L +     GA  +L  + + Y+  YFK    +        GD   R    YY   GR DD
Sbjct: 576 LCVKGSWPGAFRTLFGDHERYETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDD 635

Query: 642 TMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVFKNPSDTSPDLD 701
            +N+ G ++ + E+E     +     E A +G+     G         F    +  P  +
Sbjct: 636 VINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKGQG----IYAFVTLLEGVPYSE 695

Query: 702 KLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQ 716
           +L+ S    ++  +       R+   P LP+T + K+MRR+LR+
Sbjct: 696 ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713