MC06g0249 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0249
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAdenine nucleotide alpha hydrolases-like superfamily protein
LocationMC06: 2057842 .. 2059058 (+)
RNA-Seq ExpressionMC06g0249
SyntenyMC06g0249
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGGAAACACGCCAGATTGCAGAGGGTGAGAGTTTCTCGATCGCCGGCCTGTTTCCAGGTCTGTTTCCGGCAGATTCCAGCCAGACCCGCCGCTAATTCCATTGGGAGTTTTCAGAGGGCGGAGCTTTTAGGGCATTCCGCGGATGAGCACAGTGCAGATAGTGCGAAATATGTGGCCGAGATTGGTAATAGAATAATGGTTGTGGTTGATTCTAGTTTTGAGGGTAAAGGAGCTCTTGAATGGGCCTTGTCTCATGCGGTTCAGAGTCGGGATTCTATCGTTCTTCTTCACGTTTCCAAGCCTTCAAAACAAGGTTAAACACAAGGTCTAATGTGTGTTGATTAGCTACTTTTCGATGGAGGGTTTTTCTGATGGTGTTCTTGTTTGTGCAGGTTTGGTGTTCGATGAAAAGCTTGACATGAAGGCGTACGAGCTTCTTCTGTCTATGAAGAATATGTGCCAAATGAGAAGGCCAGGAGTAAGTTTCTGTCATGTCTTCCATTTTTTCCTTTCCCAATTGACTCCAGAGATGAAAGGAATAGTTCTTGACAGAAAAAAAAGAGTAGTTCATAATCAAATTTAGTGTAAGAATGAGCTGGTAAGACAATGGGCTCTTCATATTTCCATAGGATATTTTCTACTTTGGCCTCAGCCCATTTCTTAGAGAGGCCGAAGAACTTCCCCACTTTGAATGCATATGGAACTAGAGTTGGGCTGAGGTTTCTACCATATCAATCGTTGCCATATTATATTATCAACTCTGTGTTGTATCTGTGAATAGGTGCAAATATGAGATTCTTGGCTTAGAAATCAGTTGAATGTGGATTAAGCAGAATGAGTTAAAACATGGCACTAAAAAAGAATCCAAATGGGATTACTCTTAGAACACAAATAAATTGGGATGCGTTCCTGTTTGATTTTAGGTTCAAGTAGAGATGGAATTTCTACAGGGGAAGGAGATGGGTCGTGTGATTGTGGAAGAAGCAAAGAAACAGAGAGTGTCTCTGCTGGTTCTTGGGCAAAGAAAGCAATCTCCATTCTGGTCTCTGGTAAAGAAATTCAGCACAAGCAAGAGGAGGAATCATGGTGGGATTGTGGATTACTGTATCCAAAACTCATCATGCCCGACCATCGCGGTGAGGAGGAAAAGCAAGAAACTTGGAGGATATTTGATCACCACAAAAAGTCACAAGAATTTCTGGCTTTTGGCT

mRNA sequence

ATGGCGAGGAAACACGCCAGATTGCAGAGGGTGAGAGTTTCTCGATCGCCGGCCTGTTTCCAGGTCTGTTTCCGGCAGATTCCAGCCAGACCCGCCGCTAATTCCATTGGGAGTTTTCAGAGGGCGGAGCTTTTAGGGCATTCCGCGGATGAGCACAGTGCAGATAGTGCGAAATATGTGGCCGAGATTGGTAATAGAATAATGGTTGTGGTTGATTCTAGTTTTGAGGGTAAAGGAGCTCTTGAATGGGCCTTGTCTCATGCGGTTCAGAGTCGGGATTCTATCGTTCTTCTTCACGTTTCCAAGCCTTCAAAACAAGGTTTGGTGTTCGATGAAAAGCTTGACATGAAGGCGTACGAGCTTCTTCTGTCTATGAAGAATATGTGCCAAATGAGAAGGCCAGGAGTTCAAGTAGAGATGGAATTTCTACAGGGGAAGGAGATGGGTCGTGTGATTGTGGAAGAAGCAAAGAAACAGAGAGTGTCTCTGCTGGTTCTTGGGCAAAGAAAGCAATCTCCATTCTGGTCTCTGGTAAAGAAATTCAGCACAAGCAAGAGGAGGAATCATGGTGGGATTGTGGATTACTGTATCCAAAACTCATCATGCCCGACCATCGCGGTGAGGAGGAAAAGCAAGAAACTTGGAGGATATTTGATCACCACAAAAAGTCACAAGAATTTCTGGCTTTTGGCT

Coding sequence (CDS)

ATGGCGAGGAAACACGCCAGATTGCAGAGGGTGAGAGTTTCTCGATCGCCGGCCTGTTTCCAGGTCTGTTTCCGGCAGATTCCAGCCAGACCCGCCGCTAATTCCATTGGGAGTTTTCAGAGGGCGGAGCTTTTAGGGCATTCCGCGGATGAGCACAGTGCAGATAGTGCGAAATATGTGGCCGAGATTGGTAATAGAATAATGGTTGTGGTTGATTCTAGTTTTGAGGGTAAAGGAGCTCTTGAATGGGCCTTGTCTCATGCGGTTCAGAGTCGGGATTCTATCGTTCTTCTTCACGTTTCCAAGCCTTCAAAACAAGGTTTGGTGTTCGATGAAAAGCTTGACATGAAGGCGTACGAGCTTCTTCTGTCTATGAAGAATATGTGCCAAATGAGAAGGCCAGGAGTTCAAGTAGAGATGGAATTTCTACAGGGGAAGGAGATGGGTCGTGTGATTGTGGAAGAAGCAAAGAAACAGAGAGTGTCTCTGCTGGTTCTTGGGCAAAGAAAGCAATCTCCATTCTGGTCTCTGGTAAAGAAATTCAGCACAAGCAAGAGGAGGAATCATGGTGGGATTGTGGATTACTGTATCCAAAACTCATCATGCCCGACCATCGCGGTGAGGAGGAAAAGCAAGAAACTTGGAGGATATTTGATCACCACAAAAAGTCACAAGAATTTCTGGCTTTTGGCT

Protein sequence

MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYVAEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYELLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKKFSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Homology
BLAST of MC06g0249 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.2e-06
Identity = 49/201 (24.38%), Postives = 83/201 (41.29%), Query Frame = 0

Query: 48  SADEHSADSAKYVAEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVS------ 107
           S+   SA +    A    +I V VD S E   A+ WA+ H ++  D++VLLHVS      
Sbjct: 31  SSTPSSAATPTPTAGARRKIGVAVDLSEESSFAVRWAVDHYIRPGDAVVLLHVSPTSVLF 90

Query: 108 ------------------KPSKQGLVFDEKLDMKAYELLLSMKNMCQMRRPGVQVEMEFL 167
                             +P      FD     K  +L   +K +      G   ++  +
Sbjct: 91  GADWGPLPLKTQIEDPNAQPQPSQEDFDAFTSTKVADLAKPLKEL------GFPYKIHIV 150

Query: 168 QGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKKFSTSKRRNHGGIVDYCIQNSSCP 224
           +  +M   +  E ++  +S +++G R    F +  K+ S  K    G + DYC+ +  CP
Sbjct: 151 KDHDMRERLCLEIERLGLSAVIMGSR---GFGAEKKRGSDGKL---GSVSDYCVHHCVCP 210

BLAST of MC06g0249 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 4.0e-05
Identity = 46/193 (23.83%), Postives = 78/193 (40.41%), Query Frame = 0

Query: 47  HSADEHSADSAKYVAEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVS----- 106
           HS+   SA +    A    +I V VD S E   A+ WA+ H ++  D++V+LHVS     
Sbjct: 27  HSSSTPSAATPTPTAGARRKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSVL 86

Query: 107 --------------------------KPSKQGLVFDEKLDMKAYELLLSMKNMCQMRRPG 166
                                     KPS++   FD     K  +L   +K        G
Sbjct: 87  FGADWGPLPLQTPPPPSAATDPGAQPKPSQED--FDAFTSSKVADLAKPLK------EAG 146

Query: 167 VQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKKFSTSKRRNHGGIVDY 209
              ++  ++  +M   +  E ++  +S +++G R     +   K+ S  K    G + DY
Sbjct: 147 FPHKIHIVKDHDMRERLCLETERLNLSAVIMGSRG----FGAEKRGSDGKL---GSVSDY 204

BLAST of MC06g0249 vs. NCBI nr
Match: XP_022135256.1 (universal stress protein PHOS32-like [Momordica charantia])

HSP 1 Score: 454 bits (1169), Expect = 4.43e-161
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV
Sbjct: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE
Sbjct: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK
Sbjct: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Sbjct: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231

BLAST of MC06g0249 vs. NCBI nr
Match: XP_023531669.1 (uncharacterized protein LOC111793841 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388 bits (996), Expect = 1.03e-134
Identity = 194/231 (83.98%), Postives = 212/231 (91.77%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MA+KH RLQR+RVS+S ACF+VCF+QI  RP  + I +FQ+ ELLGH ADE+  DSAK V
Sbjct: 1   MAKKHTRLQRLRVSQSSACFKVCFQQIRTRPTGDLIKTFQKEELLGHGADEYCTDSAKSV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           AEIGNRIMVVVDSSF GKGALEWALSHAVQ++DSI+LLHV+K SKQG+VFDE LDMK YE
Sbjct: 61  AEIGNRIMVVVDSSFGGKGALEWALSHAVQNQDSIILLHVAKSSKQGVVFDEMLDMKVYE 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
            LL MKNMCQMRRPGV+VEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK
Sbjct: 121 FLLCMKNMCQMRRPGVKVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FST+KRR+HGGIV+YCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Sbjct: 181 FSTNKRRSHGGIVEYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231

BLAST of MC06g0249 vs. NCBI nr
Match: XP_022933390.1 (uncharacterized protein LOC111440820 [Cucurbita moschata] >KAG7021564.1 hypothetical protein SDJN02_15289, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 387 bits (993), Expect = 2.96e-134
Identity = 193/231 (83.55%), Postives = 212/231 (91.77%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MA+KH RLQR+RVS+S ACF++CF+QI  RP  + I +FQ+ ELLGH ADE+  DSAK V
Sbjct: 1   MAKKHTRLQRLRVSQSSACFKLCFQQIRTRPTGDLIKTFQKEELLGHGADEYCTDSAKSV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           AEIGNRIMVVVDSSF GKGALEW LSHAVQ++DSI+LLHV+K SKQG+VFDE LDMK YE
Sbjct: 61  AEIGNRIMVVVDSSFGGKGALEWVLSHAVQNQDSIILLHVAKSSKQGVVFDEMLDMKVYE 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLL MKNMCQMRRPGV+VEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK
Sbjct: 121 LLLCMKNMCQMRRPGVKVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FST+KRR+HGGIV+YCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Sbjct: 181 FSTNKRRSHGGIVEYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231

BLAST of MC06g0249 vs. NCBI nr
Match: XP_022966082.1 (uncharacterized protein LOC111465801 [Cucurbita maxima])

HSP 1 Score: 385 bits (989), Expect = 1.21e-133
Identity = 193/231 (83.55%), Postives = 212/231 (91.77%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MA+KH RLQR+RVS+S ACF+VCF+QI  RP  + I + Q+ ELLGH ADE+  DSAK V
Sbjct: 1   MAKKHTRLQRLRVSQSSACFKVCFQQIRTRPTGDLIKTCQKEELLGHGADEYCTDSAKSV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           AEIGNRIMVVVDSSF GKGALEWALSHAVQ++DSI+LLHV+K SKQG+VFDE LDMK Y+
Sbjct: 61  AEIGNRIMVVVDSSFGGKGALEWALSHAVQNQDSIILLHVAKSSKQGVVFDEMLDMKVYQ 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLL MKNMCQMRRPGV+VEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK
Sbjct: 121 LLLCMKNMCQMRRPGVKVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FST+KRR+HGGIV+YCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Sbjct: 181 FSTNKRRSHGGIVEYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231

BLAST of MC06g0249 vs. NCBI nr
Match: XP_008453308.1 (PREDICTED: uncharacterized protein LOC103494065 [Cucumis melo])

HSP 1 Score: 375 bits (964), Expect = 6.97e-130
Identity = 193/231 (83.55%), Postives = 208/231 (90.04%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MARKH RLQRVR+S+S  CFQVCFRQI  RP  +SI +FQR ELLG   D +   S K V
Sbjct: 1   MARKHTRLQRVRISQSSGCFQVCFRQIRTRPTVDSIQNFQREELLG---DGYCTGSVKSV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           A+IGN+IMVVVDSSFEGKGALEWALSHAVQS DSI+LLHVSK SKQG+VFDEKLDMKAY+
Sbjct: 61  AQIGNKIMVVVDSSFEGKGALEWALSHAVQSHDSIILLHVSKSSKQGVVFDEKLDMKAYQ 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLLS+KNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPF SL+KK
Sbjct: 121 LLLSLKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFRSLIKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FST+KRRNHGGIV+YCIQ SSC TIAVRRKSKK+GGYLITTKSHKNFWLLA
Sbjct: 181 FSTNKRRNHGGIVEYCIQTSSCLTIAVRRKSKKVGGYLITTKSHKNFWLLA 228

BLAST of MC06g0249 vs. ExPASy TrEMBL
Match: A0A6J1C057 (universal stress protein PHOS32-like OS=Momordica charantia OX=3673 GN=LOC111007264 PE=4 SV=1)

HSP 1 Score: 454 bits (1169), Expect = 2.14e-161
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV
Sbjct: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE
Sbjct: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK
Sbjct: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Sbjct: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231

BLAST of MC06g0249 vs. ExPASy TrEMBL
Match: A0A6J1F4R8 (uncharacterized protein LOC111440820 OS=Cucurbita moschata OX=3662 GN=LOC111440820 PE=4 SV=1)

HSP 1 Score: 387 bits (993), Expect = 1.43e-134
Identity = 193/231 (83.55%), Postives = 212/231 (91.77%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MA+KH RLQR+RVS+S ACF++CF+QI  RP  + I +FQ+ ELLGH ADE+  DSAK V
Sbjct: 1   MAKKHTRLQRLRVSQSSACFKLCFQQIRTRPTGDLIKTFQKEELLGHGADEYCTDSAKSV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           AEIGNRIMVVVDSSF GKGALEW LSHAVQ++DSI+LLHV+K SKQG+VFDE LDMK YE
Sbjct: 61  AEIGNRIMVVVDSSFGGKGALEWVLSHAVQNQDSIILLHVAKSSKQGVVFDEMLDMKVYE 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLL MKNMCQMRRPGV+VEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK
Sbjct: 121 LLLCMKNMCQMRRPGVKVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FST+KRR+HGGIV+YCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Sbjct: 181 FSTNKRRSHGGIVEYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231

BLAST of MC06g0249 vs. ExPASy TrEMBL
Match: A0A6J1HM19 (uncharacterized protein LOC111465801 OS=Cucurbita maxima OX=3661 GN=LOC111465801 PE=4 SV=1)

HSP 1 Score: 385 bits (989), Expect = 5.83e-134
Identity = 193/231 (83.55%), Postives = 212/231 (91.77%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MA+KH RLQR+RVS+S ACF+VCF+QI  RP  + I + Q+ ELLGH ADE+  DSAK V
Sbjct: 1   MAKKHTRLQRLRVSQSSACFKVCFQQIRTRPTGDLIKTCQKEELLGHGADEYCTDSAKSV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           AEIGNRIMVVVDSSF GKGALEWALSHAVQ++DSI+LLHV+K SKQG+VFDE LDMK Y+
Sbjct: 61  AEIGNRIMVVVDSSFGGKGALEWALSHAVQNQDSIILLHVAKSSKQGVVFDEMLDMKVYQ 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLL MKNMCQMRRPGV+VEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK
Sbjct: 121 LLLCMKNMCQMRRPGVKVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FST+KRR+HGGIV+YCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA
Sbjct: 181 FSTNKRRSHGGIVEYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231

BLAST of MC06g0249 vs. ExPASy TrEMBL
Match: A0A1S3BVX2 (uncharacterized protein LOC103494065 OS=Cucumis melo OX=3656 GN=LOC103494065 PE=4 SV=1)

HSP 1 Score: 375 bits (964), Expect = 3.38e-130
Identity = 193/231 (83.55%), Postives = 208/231 (90.04%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPAANSIGSFQRAELLGHSADEHSADSAKYV 60
           MARKH RLQRVR+S+S  CFQVCFRQI  RP  +SI +FQR ELLG   D +   S K V
Sbjct: 1   MARKHTRLQRVRISQSSGCFQVCFRQIRTRPTVDSIQNFQREELLG---DGYCTGSVKSV 60

Query: 61  AEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAYE 120
           A+IGN+IMVVVDSSFEGKGALEWALSHAVQS DSI+LLHVSK SKQG+VFDEKLDMKAY+
Sbjct: 61  AQIGNKIMVVVDSSFEGKGALEWALSHAVQSHDSIILLHVSKSSKQGVVFDEKLDMKAYQ 120

Query: 121 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 180
           LLLS+KNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPF SL+KK
Sbjct: 121 LLLSLKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFRSLIKK 180

Query: 181 FSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 231
           FST+KRRNHGGIV+YCIQ SSC TIAVRRKSKK+GGYLITTKSHKNFWLLA
Sbjct: 181 FSTNKRRNHGGIVEYCIQTSSCLTIAVRRKSKKVGGYLITTKSHKNFWLLA 228

BLAST of MC06g0249 vs. ExPASy TrEMBL
Match: A0A6P9ELK1 (uncharacterized protein LOC109010079 OS=Juglans regia OX=51240 GN=LOC109010079 PE=4 SV=1)

HSP 1 Score: 245 bits (626), Expect = 1.43e-78
Identity = 138/247 (55.87%), Postives = 175/247 (70.85%), Query Frame = 0

Query: 1   MARKHARLQRVRVSRSPACFQVCFRQIPARPA-ANSIGSFQRAELLGHSADEHSADSAK- 60
           M RK  R+  + V R+ ACF+V      ++P   +SI + Q+ E L +S ++H+  SA  
Sbjct: 1   MGRKRVRVASLSVGRATACFRVGSTPFQSKPRNLDSIEAAQKTEFLSNSREDHNEFSAGG 60

Query: 61  -------------YVAEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSK 120
                          AE+GN+IMVVVDSS E +GALEWALSH VQS DSI+LLHVSK SK
Sbjct: 61  GPEDPDRQVKDHGVPAEVGNKIMVVVDSSLEAQGALEWALSHTVQSHDSIILLHVSKLSK 120

Query: 121 QGLVFDEKLDMKAYELLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLV 180
           QG     K  + A+ELL SMKNMCQM+RPGVQVE+  ++G+E G +IVEEA++QRVSLLV
Sbjct: 121 QGS--KGKGSLMAHELLYSMKNMCQMKRPGVQVEIALVEGREKGPIIVEEARQQRVSLLV 180

Query: 181 LGQRKQSPF-WSLVKKFSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSH 231
           +GQ KQS   WSL+K+++   +RN GG+ +YCIQN+ C TIAVRRKS+KLGGYLITTK H
Sbjct: 181 IGQGKQSSMLWSLMKRWAG--KRNRGGVAEYCIQNAYCMTIAVRRKSRKLGGYLITTKRH 240

BLAST of MC06g0249 vs. TAIR 10
Match: AT5G17390.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 193.4 bits (490), Expect = 2.1e-49
Identity = 90/181 (49.72%), Postives = 137/181 (75.69%), Query Frame = 0

Query: 53  SADSAKYVAEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDE 112
           + ++ +  AE GNR+MVVVD +    GALEWA++H +Q +D++ LL+ +KP ++    + 
Sbjct: 105 AVEAVEEEAEGGNRVMVVVDKALASTGALEWAITHTLQPQDTLFLLYFAKPFRKSKRKNR 164

Query: 113 KLDMKAYELLLSMKNMCQMRRPGVQVEMEFLQG--KEMGRVIVEEAKKQRVSLLVLGQRK 172
           K ++K  EL+ ++K +CQ +RPG++VE+  L+G  K+ G+ IVEE+KKQ+VSLLV+GQ K
Sbjct: 165 KREVKTDELVHTLKKLCQTKRPGIEVEIRRLEGKDKDKGQKIVEESKKQQVSLLVVGQEK 224

Query: 173 QSPFWSLVKKFSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLL 232
           + P W L+K+++  +RR H G++ YC++N+SC TIAV+ K++KLGGYLITTK HKNFWLL
Sbjct: 225 KPPVWRLLKRWAWKRRRGHEGVLKYCLENASCMTIAVKPKNRKLGGYLITTKRHKNFWLL 284

BLAST of MC06g0249 vs. TAIR 10
Match: AT3G03290.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 4.6e-49
Identity = 93/174 (53.45%), Postives = 127/174 (72.99%), Query Frame = 0

Query: 60  VAEIGNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDEKLDMKAY 119
           + E GNR+MVVVD      GALEWAL H +QS+D + LL+ SKP ++G   + K ++K  
Sbjct: 101 ITEAGNRVMVVVDKVIASTGALEWALKHTLQSQDYLFLLYFSKPFRKGKRKNRKSEVKTD 160

Query: 120 ELLLSMKNMCQMRRPGVQVEMEFLQG--KEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSL 179
           EL+ ++K +CQ +RPG++VE+  LQG  KE G  IVEEAK+Q+VSLLV+G+ K+ P W L
Sbjct: 161 ELVHTLKKLCQTKRPGIEVEIRRLQGKEKEKGEKIVEEAKEQQVSLLVVGKEKKPPVWRL 220

Query: 180 VKKFSTSKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 232
           +K++   KRR   G + YC++ +SC TIAV+ K++KLGGYLITTK HKNFWLLA
Sbjct: 221 LKRWGWKKRRGRAGTLKYCLEKASCMTIAVKPKNRKLGGYLITTKRHKNFWLLA 274

BLAST of MC06g0249 vs. TAIR 10
Match: AT2G03720.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.1e-37
Identity = 83/168 (49.40%), Postives = 118/168 (70.24%), Query Frame = 0

Query: 68  MVVVDSSFEGKGALEWALSHAVQSRDSIVLLHVSKPSKQGLVFDE---KLDMKAYELLLS 127
           MVVVD++ + K AL+WAL+H VQ  D+I LLHV++ +  G   DE   + + +A+EL+  
Sbjct: 1   MVVVDTTSQTKNALQWALTHCVQDEDNITLLHVTR-TPVGQAIDETQRERNSRAHELVHP 60

Query: 128 MKNMCQMRRPGVQVEMEFLQ-GKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKKFST 187
           +KN CQ+++P V+ E+  ++  +E G+ IVEE+KKQ   +LVLGQRK++  W ++ K+ T
Sbjct: 61  LKNFCQLKKPNVKTEIVVVETAEEKGKTIVEESKKQGAGVLVLGQRKRTSKWRVIWKWRT 120

Query: 188 SKRRNHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 232
            K    GG+V+YCI NS C  IAVR+KS   GGYLITTK HK+FWLLA
Sbjct: 121 -KGGMGGGVVEYCIHNSDCMAIAVRKKSNN-GGYLITTKRHKDFWLLA 165

BLAST of MC06g0249 vs. TAIR 10
Match: AT1G69080.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 2.0e-36
Identity = 84/181 (46.41%), Postives = 116/181 (64.09%), Query Frame = 0

Query: 64  GNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHV--SKPSKQGLVF------DEKLD 123
           G RI+VVVDS  E K AL W LSH  Q +DSI+LLH   +K S+ G +       DE  D
Sbjct: 43  GRRIIVVVDSCSEAKNALLWTLSHCAQPQDSILLLHFLKAKTSQSGDLANKEEGEDESCD 102

Query: 124 ----MKAYELLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQ 183
                +A + + ++K MC+++RP V+ E+ F++G E G  IV+EA+++  SLLVLGQ+KQ
Sbjct: 103 KPTTSRADKKVSALKTMCELKRPEVKTEVVFVKGDEKGPTIVKEAREREASLLVLGQKKQ 162

Query: 184 SPFWSLVKKFSTSKRR-NHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLL 232
              W L+  +++  R       V+YCI NS C  IAVR++ KKLGGY +TTK HK+FWLL
Sbjct: 163 HATWRLLMVWASQARPVTKHDFVEYCINNSPCMAIAVRKRGKKLGGYTLTTKRHKDFWLL 222

BLAST of MC06g0249 vs. TAIR 10
Match: AT1G69080.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 7.4e-31
Identity = 78/172 (45.35%), Postives = 106/172 (61.63%), Query Frame = 0

Query: 64  GNRIMVVVDSSFEGKGALEWALSHAVQSRDSIVLLHV--SKPSKQG-LVFDEKLDMKAYE 123
           G RI+VVVDS  E K AL W LSH  Q +DSI+LLH   +K S+ G L   E+ + ++ +
Sbjct: 43  GRRIIVVVDSCSEAKNALLWTLSHCAQPQDSILLLHFLKAKTSQSGDLANKEEGEDESCD 102

Query: 124 LLLSMKNMCQMRRPGVQVEMEFLQGKEMGRVIVEEAKKQRVSLLVLGQRKQSPFWSLVKK 183
                K         V+ E+ F++G E G  IV+EA+++  SLLVLGQ+KQ   W L+  
Sbjct: 103 -----KPTTSRADKKVKTEVVFVKGDEKGPTIVKEAREREASLLVLGQKKQHATWRLLMV 162

Query: 184 FSTSKRR-NHGGIVDYCIQNSSCPTIAVRRKSKKLGGYLITTKSHKNFWLLA 232
           +++  R       V+YCI NS C  IAVR++ KKLGGY +TTK HK+FWLLA
Sbjct: 163 WASQARPVTKHDFVEYCINNSPCMAIAVRKRGKKLGGYTLTTKRHKDFWLLA 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYN96.2e-0624.38Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S... [more]
Q8L4N14.0e-0523.83Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S... [more]
Match NameE-valueIdentityDescription
XP_022135256.14.43e-161100.00universal stress protein PHOS32-like [Momordica charantia][more]
XP_023531669.11.03e-13483.98uncharacterized protein LOC111793841 [Cucurbita pepo subsp. pepo][more]
XP_022933390.12.96e-13483.55uncharacterized protein LOC111440820 [Cucurbita moschata] >KAG7021564.1 hypothet... [more]
XP_022966082.11.21e-13383.55uncharacterized protein LOC111465801 [Cucurbita maxima][more]
XP_008453308.16.97e-13083.55PREDICTED: uncharacterized protein LOC103494065 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1C0572.14e-161100.00universal stress protein PHOS32-like OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A6J1F4R81.43e-13483.55uncharacterized protein LOC111440820 OS=Cucurbita moschata OX=3662 GN=LOC1114408... [more]
A0A6J1HM195.83e-13483.55uncharacterized protein LOC111465801 OS=Cucurbita maxima OX=3661 GN=LOC111465801... [more]
A0A1S3BVX23.38e-13083.55uncharacterized protein LOC103494065 OS=Cucumis melo OX=3656 GN=LOC103494065 PE=... [more]
A0A6P9ELK11.43e-7855.87uncharacterized protein LOC109010079 OS=Juglans regia OX=51240 GN=LOC109010079 P... [more]
Match NameE-valueIdentityDescription
AT5G17390.12.1e-4949.72Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G03290.14.6e-4953.45Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT2G03720.11.1e-3749.40Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT1G69080.12.0e-3646.41Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT1G69080.27.4e-3145.35Adenine nucleotide alpha hydrolases-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006016UspAPFAMPF00582Uspcoord: 66..208
e-value: 2.0E-23
score: 83.5
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 64..215
e-value: 3.6E-22
score: 81.1
NoneNo IPR availablePANTHERPTHR47000ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEINcoord: 1..231
NoneNo IPR availableCDDcd00293USP_Likecoord: 66..207
e-value: 2.98349E-22
score: 86.2698
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 65..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0249.1MC06g0249.1mRNA