MC06g0032 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0032
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPseudouridine synthase
LocationMC06: 208075 .. 212737 (-)
RNA-Seq ExpressionMC06g0032
SyntenyMC06g0032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACTATTTAGTTGTCGAACCGAGGAAGAAGACAAAGTAAGCGCTCGCGAAGGCAGACTGATTCGAAATCCACGCGGTCTTGACTTTATGTCACGTCAGTACTTGTGTTTAGTTCGGTTCGAAACAAATACGACGTCGCAGGGTTTAGGAACCATACGGCGTCGTTTTGGTCGTTCTCTCCGGTCAAAAGGTAAAACTCCGAGTGCGAGTGCGGGATGGGGAGCAAGAGAAAGAGGGAAGAAGAGGACATGGCAGTGGGAGAGATCGTATGGCAAACCCCTGCCAACCCCCCACAACGAAGCGATTACATACTTCTAAATGGTACAAATGAAAAGCCCTCTTTCTGGGTTTTTGTTTTCGTTTTACATTTCAGCATGTTTTAATCAGAGTTCTTTGATTAGGGAGACGGCATGTTAGACCCTACTACTTCGAGTTCATTGCTCATGTAAGTGACTTGTAGCCTCTCTTTCGTATTTTACAGTTGAACCGAAATCCAAATTGGAAGTTTATATCTGCAATTTGCAGGTTAAGAATCGATGGGCCGGAAAAACCATTGTCGACTTGTTTGCTGAAGAGTTTAAAGGCCGACCTTATGAGTACTATGTACACTTTTTCCTTTCCGTTTTGCTTGTATGATGTGATTATTTGGTTTCAGCTTTTGTTTGTTATTTTATTGTTTTGTTTGAATCAAAATATGTATTAGCCACGTGGACTGAATTATTTTCATTCCCTCGTAGATTTTGCCTTGTTTTCATTCCTTACTGGTTGGAACTCTGTTTCATAGATTGATGCTGTCAAATGTGGTCGTATACAAGTCGATGGGGAGCTGGTGCCTGTATCATATATAGTTAGATCATCTCAAAAGATAAGTCATTTCCTGCACAGGTATGTGGAGATTGCAAGTTCAATAAAGGCTTACAGTTTCACGATTGGTTTAATTTGGTTGAACTTACATTTGTTCCCAAGCGGGCTGCCCATGATTCCCTTGAATTTACATTTGTTAAGCTTTCTTTTGGTAAAAGCTGCACTATAGAGTTTATCAAAAAATTGTAATTGATTTCACAAGGGCTGTAGGCATGAACCGCCAGTGATGGCTTGTGATGTCAAAATTCTCAAAGAAGAAGCAGATGTGCTGACGGTTTGCAAGCCTGCATCTGTTCCAGTGAGTTTTATTGTAGTCTGTGCTTTATATTACATCATTAGTTATGCAAATAGTGCAATACATGTCCCGTAAACCTTTTTTTTTAGTGTATCTATTCCTCTTGTTTGCTACTGTTTGTTTTTCTTTTAAGTTGATTTTACCATCACTTTTAGTTCCATTTAACTTGCAAGCTCCAATGTAATATAGGTCCATCCATGTGGTCAATATAGGAAGAACACTGTTGTTGGTATACTTCAGGCAGAGCATGGTTTGGCACCTCTATTTCGTATCCAACTTCCATGTCCTTCTAGAAGCTATTATTCTCATGATTTAGCTCTGTTGAATTGATAATGAAACAAACTCTGTTAGCAAATTTTGTCCACTGGTTTGAAGGGCAAACCTCTTCCTTCCTTGACATAACCATGTCAGCTATACATCGACTAGACCGCCTTGTCTCAGGACTTCTCATCCTTGCTAGGAATTCTTCCAAAGCTGATCTTTTCAGACAACAGGTATTCCTCATACTTGAGAGTTTTTTACATTAGTTCTATTCTTTAGTGACAGTAAATAAAAAAAGGCATGAAATTTTTATGAGGTGTATTCTTCTGAAACATTTTAATCCAACTTGTACAATAGCTTTTCTTCAGTTGACAACTTCTAATTATTATGTAATTATATATTGTTGAGAACTGATTTACTTTACATTTACAACATTGCAGTGCTCGTAGTTGGACAATGCCTGGACTGACATTGTCAGTAAATAAAAGTTTAAATTAGAGAACCAGCAATAATTAATATTAATGGTTATATGATTTAATATAAAGATAATATGAGATAAAAAAAAGCTACTGATTGCACTTAAAGGATTATATAAATATCAAAGCCTTTACTATGAAATGGGTTGAATATGATTCTTACTTACAAAATATACAAATAATTATGGTGATAAATTGGTATGGCGATTACACAATTGGTAAGAAAAATGTAAGTTCATAAAATTTGAAAAATTTAGATGATATGTGGATTGAAATTTTTAAAAAACGTTTGAAACTGAAGAAAAGAGTATATCCTGATTAATAATTAGTCGTTTGTTAAACAGTAAAATTGTAAATAGAAATGTGAAAACCTAGGTAAATATCATAGAATACTAGATGATTTTTGAAAAAACGTTGACAAAAGATTGAACTCTAAATTACTTTTGCAGCTAAAGTGAAATTTTGTATAATTATAAAATCAACCAGAAATAAATATAAAACATATCACAGATTCACATATGGTGTAGTACCTGTAAAGATGGAAGGAAAATTATCTTTGAAGAAGACAAATGGTAGTTGTAATGATTATTTAATATGAAAATTAAATAATAAGAGAGAATTTGAAACAAAAATAATAAAAATTTCAATCTATGTAAAGATGCGATTTAAAGATTAAAAAAGTCCACTTGCATTGTTTCACTGTGTATTGTTAAATTCAATATGTGATTGATTTCACGTTCTTTTCGGCATATACACAAACTAGAAACTGAAGAAACTCATATATTAGGAATGTGTGCATATTGCAGATTGAGTCTGGGCTGGTGAAGAAACAGTACATTGCAAGGGTAGTGGGCGTATTTCCAGAGAAAGAGGTATTTCTTCTTTGGGCTAGTCAAGTCATGCATTTTCAAATTGTTCCAACTAATATGACCAGACCAGACTTAAGGGCTATACTTGGTCCTGGCTGCTCTAGGATCCTCTTTTCTGGCTTTTACCCATTGGGATGATGGGAATAAAGCCGTGGAATGTGGAACGTATTCAGACATGTACGTTGTGGGTCTGTGCTTAAAATCTTCCACAATTAGGTTGACTCTGCCTCTTAAAAGAGAGTGGTATATACTTTTTTGAATTCAAACAAGAGGGTGGGGAGTTTGAACCTCCAAGCTGTATGGAGGCCAGAGAAATTCTTGTATTATAGGCAGAGGAAAATGTTGTCAATGGAGATGTGCTCGAGTGTTTGTGGCTTGTGCTCTTTAATAGGCCATGCGGAATTCTAAAAGGAGTATGAAATAAAACACCGAGTTGCTTTTGACACGAGGGGGAAGCATGTTATCAAAATATTCTCAATCTCAAGCATGATATCCAGCCATCTTGGAGTACTGTGCGATTATGTTGTATCTTTATGTTCAGTGAAGTCACATCATAATACTGTTTATTCGATTTCTATTATGCATCTCTCTGATTATCTGCTTCATTTTCCCCTGGACTTAATTTCAGCAACTTGTTGATGTTAACATAAATTACAATGCTCGAGAAGGAAGGAGCACAGCAGAGGTGAGGCTTCATTTTTACCCTTGTTTATATGGCACTTCACTTGCTCAAGGAGTGAGAGTGAGTGATAAGGATGAGATTTACTTTGTAGCAGGATATTGTCCTGTTTTGATACAAGTAGTCTGCAAAAGCTTCTTAATTACTGAAAACTGTTGTTTTTTAATTCCATAATATTGACTTGGATAACAAGGGTGGCCAGTCACAGTCACTATTGGAATTGGAGTCATGTTGACTGGAAATTTGAGTTGCTTGCTCTTGAAACATTATTATAAAACAACTCGAAGTATATTGGGTTTAATTTAGTAGTGTTGTTTGATACCACTAGCAGATGGTTGATTCTTGCTCGGATGCTCGAACAAAGGGGAAGACCGCTCAAACGAAGTTTACCCGTATCAGTTCGGATGGAATTCATAGCATTGTTTTGTGCGAACCGATCACTGGGCGAACTCATCAAGTAAAATTCCAGTCCTGACTTATTGAAGGGGAAGGGGGTGGCATGCCCCATAATGTTATTTCATGTGTTAAAATGAGTGTGTTGTGGTAATGCAGATACGTGTGCATTTGCAGTACAGTGGGTATCCTATAGCGAATGACGCACTGTACCTCACAAAAGAAGCTTCTTGTCGTTCGGTGGAAAGAACAAATGCAGATAGAGCAGCAGCCCATTGTCCAGCACGTGATACTGAAGAAGATTGCGTTGATGGATGTAAAGAGAAGTCAGGTGAAGATTTTAGCATCGACTCCATGTGTACGAACTGTCCAAATTTGGGCCCAAAGGGGTAAGAAGAAACTTATATCCCACGCAGGTTATAGCTTGATTTTAGCATTAACCGTGGTTTGTTTGTTTGTTATGTGAAGATACGAGGGAGACGAAGAGGGTTTATGGCTGCATTGTGTTCGGTATTCTGGACCAGGATGGATCTACGAATGCCCATATCCAGAATGGGCTTCATTTTAATACACAGCCTCGCCATTCTCTGTATTTGTTATCAATGCTACAGCCCACAGTGATTTCGCTTTGGCCTGGGAAAGCCATTTTGGAAGGCTGGGTCGGGATTTCAATGTGATTCTTCCTGTCCCACATCTTAACAGTGTGTTTAGGGTGTCTTCACTTAGAATATTTGTATTAAATTTAATTTTATCATGCAAAGCATAATTTAATGTGTCACAAGTTTTCATTCTTTTCCCACAGAGGCTTTAATTTTATTGCTACTCC

mRNA sequence

ATACTATTTAGTTGTCGAACCGAGGAAGAAGACAAAGTAAGCGCTCGCGAAGGCAGACTGATTCGAAATCCACGCGGTCTTGACTTTATGTCACGTCAGTACTTGTGTTTAGTTCGGTTCGAAACAAATACGACGTCGCAGGGTTTAGGAACCATACGGCGTCGTTTTGGTCGTTCTCTCCGGTCAAAAGGTAAAACTCCGAGTGCGAGTGCGGGATGGGGAGCAAGAGAAAGAGGGAAGAAGAGGACATGGCAGTGGGAGAGATCGTATGGCAAACCCCTGCCAACCCCCCACAACGAAGCGATTACATACTTCTAAATGGGAGACGGCATGTTAGACCCTACTACTTCGAGTTCATTGCTCATGTTAAGAATCGATGGGCCGGAAAAACCATTGTCGACTTGTTTGCTGAAGAGTTTAAAGGCCGACCTTATGAGTACTATATTGATGCTGTCAAATGTGGTCGTATACAAGTCGATGGGGAGCTGGTGCCTGTATCATATATAGTTAGATCATCTCAAAAGATAAGTCATTTCCTGCACAGGCATGAACCGCCAGTGATGGCTTGTGATGTCAAAATTCTCAAAGAAGAAGCAGATGTGCTGACGGTTTGCAAGCCTGCATCTGTTCCAGTCCATCCATGTGGTCAATATAGGAAGAACACTGTTGTTGGTATACTTCAGGCAGAGCATGGTTTGGCACCTCTATTTCCTATACATCGACTAGACCGCCTTGTCTCAGGACTTCTCATCCTTGCTAGGAATTCTTCCAAAGCTGATCTTTTCAGACAACAGATTGAGTCTGGGCTGGTGAAGAAACAGTACATTGCAAGGGTAGTGGGCGTATTTCCAGAGAAAGAGCAACTTGTTGATGTTAACATAAATTACAATGCTCGAGAAGGAAGGAGCACAGCAGAGCAGATGGTTGATTCTTGCTCGGATGCTCGAACAAAGGGGAAGACCGCTCAAACGAAGTTTACCCGTATCAGTTCGGATGGAATTCATAGCATTGTTTTGTGCGAACCGATCACTGGGCGAACTCATCAAATACGTGTGCATTTGCAGTACAGTGGGTATCCTATAGCGAATGACGCACTGTACCTCACAAAAGAAGCTTCTTGTCGTTCGGTGGAAAGAACAAATGCAGATAGAGCAGCAGCCCATTGTCCAGCACGTGATACTGAAGAAGATTGCGTTGATGGATGTAAAGAGAAGTCAGGTGAAGATTTTAGCATCGACTCCATGTGTACGAACTGTCCAAATTTGGGCCCAAAGGGATACGAGGGAGACGAAGAGGGTTTATGGCTGCATTGTGTTCGGTATTCTGGACCAGGATGGATCTACGAATGCCCATATCCAGAATGGGCTTCATTTTAATACACAGCCTCGCCATTCTCTGTATTTGTTATCAATGCTACAGCCCACAGTGATTTCGCTTTGGCCTGGGAAAGCCATTTTGGAAGGCTGGGTCGGGATTTCAATGTGATTCTTCCTGTCCCACATCTTAACAGTGTGTTTAGGGTGTCTTCACTTAGAATATTTGTATTAAATTTAATTTTATCATGCAAAGCATAATTTAATGTGTCACAAGTTTTCATTCTTTTCCCACAGAGGCTTTAATTTTATTGCTACTCC

Coding sequence (CDS)

ATGGGGAGCAAGAGAAAGAGGGAAGAAGAGGACATGGCAGTGGGAGAGATCGTATGGCAAACCCCTGCCAACCCCCCACAACGAAGCGATTACATACTTCTAAATGGGAGACGGCATGTTAGACCCTACTACTTCGAGTTCATTGCTCATGTTAAGAATCGATGGGCCGGAAAAACCATTGTCGACTTGTTTGCTGAAGAGTTTAAAGGCCGACCTTATGAGTACTATATTGATGCTGTCAAATGTGGTCGTATACAAGTCGATGGGGAGCTGGTGCCTGTATCATATATAGTTAGATCATCTCAAAAGATAAGTCATTTCCTGCACAGGCATGAACCGCCAGTGATGGCTTGTGATGTCAAAATTCTCAAAGAAGAAGCAGATGTGCTGACGGTTTGCAAGCCTGCATCTGTTCCAGTCCATCCATGTGGTCAATATAGGAAGAACACTGTTGTTGGTATACTTCAGGCAGAGCATGGTTTGGCACCTCTATTTCCTATACATCGACTAGACCGCCTTGTCTCAGGACTTCTCATCCTTGCTAGGAATTCTTCCAAAGCTGATCTTTTCAGACAACAGATTGAGTCTGGGCTGGTGAAGAAACAGTACATTGCAAGGGTAGTGGGCGTATTTCCAGAGAAAGAGCAACTTGTTGATGTTAACATAAATTACAATGCTCGAGAAGGAAGGAGCACAGCAGAGCAGATGGTTGATTCTTGCTCGGATGCTCGAACAAAGGGGAAGACCGCTCAAACGAAGTTTACCCGTATCAGTTCGGATGGAATTCATAGCATTGTTTTGTGCGAACCGATCACTGGGCGAACTCATCAAATACGTGTGCATTTGCAGTACAGTGGGTATCCTATAGCGAATGACGCACTGTACCTCACAAAAGAAGCTTCTTGTCGTTCGGTGGAAAGAACAAATGCAGATAGAGCAGCAGCCCATTGTCCAGCACGTGATACTGAAGAAGATTGCGTTGATGGATGTAAAGAGAAGTCAGGTGAAGATTTTAGCATCGACTCCATGTGTACGAACTGTCCAAATTTGGGCCCAAAGGGATACGAGGGAGACGAAGAGGGTTTATGGCTGCATTGTGTTCGGTATTCTGGACCAGGATGGATCTACGAATGCCCATATCCAGAATGGGCTTCATTTTAA

Protein sequence

MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWASF
Homology
[Close]
BLAST of MC06g0032 vs. ExPASy Swiss-Prot
Match: Q0E0Y3 (RNA pseudouridine synthase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0512300 PE=2 SV=2)

HSP 1 Score: 535.0 bits (1377), Expect = 6.9e-151
Identity = 259/384 (67.45%), Postives = 306/384 (79.69%), Query Frame = 0 

Query: 12  MAVGE--IVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFK 71
           MA G   IVWQTPANPP+R DYI  +GRR+VRPYYFEFI+HVKNRWAGKTIVDLF +EFK
Sbjct: 1   MAAGPAGIVWQTPANPPERQDYIFRDGRRYVRPYYFEFISHVKNRWAGKTIVDLFTDEFK 60

Query: 72  GRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDVKILKEEADV 131
           GRP EYY+ AVKCGR+QVD ++V   Y+V+SSQKISHFLHRHEPPV+  D+ IL+ EADV
Sbjct: 61  GRPREYYVHAVKCGRLQVDDQMVHADYVVQSSQKISHFLHRHEPPVLGGDITILQNEADV 120

Query: 132 LTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLILARNSSKADL 191
           +TVCKPASVPVHPCGQYRKNTVVGILQAEHGL PLFP+HRLDRLVSGLLI A+N+ KA+ 
Sbjct: 121 VTVCKPASVPVHPCGQYRKNTVVGILQAEHGLVPLFPVHRLDRLVSGLLIFAKNADKAES 180

Query: 192 FRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVDSCSDARTKGKT 251
           FRQQIE+ L++K+Y+A+VVGVFP+ EQ V+ N+++NAREGRSTAE + D    A   GK 
Sbjct: 181 FRQQIEASLLQKEYVAKVVGVFPDGEQTVNANVHFNAREGRSTAE-VCDGDGKAPI-GKQ 240

Query: 252 AQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEASCRSVERTN 311
           A TKF RI +DGIHSIVLC+P+TGRTHQIRVHL++ GYPIAND +YL++  S RS + T 
Sbjct: 241 ACTKFQRICTDGIHSIVLCKPVTGRTHQIRVHLKHIGYPIANDEVYLSENFSPRSSKGTR 300

Query: 312 ADRA---AAHCPARDTEEDCVDGCKEKSGED------FSIDSMCTNCPNLGPKGYEGDEE 371
            +RA   A   P+ D  + C D     + ED      FSID MCTNCPNL P GY+ DEE
Sbjct: 301 INRATTLACSLPSSD-PDSCADLGNNDTNEDTEADEEFSIDPMCTNCPNLAPVGYDADEE 360

Query: 372 GLWLHCVRYSGPGWIYECPYPEWA 385
            LWLHCVRY+GP W YECPYP+WA
Sbjct: 361 ALWLHCVRYTGPDWSYECPYPDWA 381

[Close]
BLAST of MC06g0032 vs. ExPASy Swiss-Prot
Match: Q9LU60 (RNA pseudouridine synthase 7 OS=Arabidopsis thaliana OX=3702 GN=At5g51140 PE=2 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.1e-143
Identity = 253/400 (63.25%), Postives = 296/400 (74.00%), Query Frame = 0 

Query: 4   KRKREEEDMAVG------------------EIVWQTPANPPQRSDYILLNGRRHVRPYYF 63
           KRK++E+D   G                  +++ Q+ +   Q+ DYI   GRRHVRPYYF
Sbjct: 2   KRKQQEDDNDDGVEKAVSPVLATTSNTMSTDLLLQSSSKLSQKQDYIFHGGRRHVRPYYF 61

Query: 64  EFIAHVKNRWAGKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKIS 123
           EFI+HV  RW GKTIVDLFA+EFKGRP +YY+ AVK GRI+VDGE+VPVSYIV+SSQKI+
Sbjct: 62  EFISHVNKRWTGKTIVDLFADEFKGRPRDYYVGAVKSGRIKVDGEIVPVSYIVKSSQKIT 121

Query: 124 HFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLF 183
           HFLHRHEPPVM  DV IL +E DV+TVCKPASVPVHPCGQYRKNT+VGIL AEH L  LF
Sbjct: 122 HFLHRHEPPVMIDDVVILHQEPDVVTVCKPASVPVHPCGQYRKNTIVGILDAEHDLGTLF 181

Query: 184 PIHRLDRLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYN 243
           PIHRLDRLVSGLLI+AR ++KAD FRQQIE G+VKK+YIA+V+GVFPE E +VD NINYN
Sbjct: 182 PIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANINYN 241

Query: 244 AREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYS 303
             EGRSTAE    S  D + KGK A TKFTRI ++G HS+V CEP+TGRTHQIRVHLQY+
Sbjct: 242 GSEGRSTAEDANSSGDDKKVKGKPACTKFTRIDTNGTHSLVSCEPVTGRTHQIRVHLQYT 301

Query: 304 GYPIANDALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCT 363
           G+PIAND LYL +     ++E   A R  A      + +D V      S EDFSID MCT
Sbjct: 302 GHPIANDPLYLNQHID--NLETYIAKRIDAGERKIVSPDDYV-----YSSEDFSIDPMCT 361

Query: 364 NCPNLGPKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWAS 386
           NCP L P+GYE  +E LWLHCV+Y G GW YECPYP WAS
Sbjct: 362 NCPKLIPQGYEEHDEALWLHCVQYCGTGWEYECPYPSWAS 394

[Close]
BLAST of MC06g0032 vs. ExPASy Swiss-Prot
Match: Q09709 (Uncharacterized protein C18B11.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC18B11.02c PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.9e-60
Identity = 133/382 (34.82%), Postives = 200/382 (52.36%), Query Frame = 0 

Query: 24  NPPQRSDYILL-NGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYYIDAVKC 83
           +P +  +Y+   +G R V PYY+E+I   K RW GKT++++F  EF+ R   YY  A++ 
Sbjct: 8   DPLKDCEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRN 67

Query: 84  GRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHP 143
           G+++V+ ++  V  ++ +   +SH  HRHEPPV    V I+ E+   + + KPA VPVHP
Sbjct: 68  GQVKVNNQIGNVDTLIENGYIVSHHAHRHEPPVSDQPVGIVHEDESYVVIDKPAGVPVHP 127

Query: 144 CGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLILARNSSKADLFRQQIESGLVKKQ 203
            G+Y  NTV+ IL  E+  + L+P +RLDRL SGL+  +++   A+  R  + S  +KK+
Sbjct: 128 TGRYNHNTVLHILMKENKCSLLYPCNRLDRLTSGLMFFSKSPKAAEEMRVHMISKSLKKE 187

Query: 204 YIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGI 263
           Y+ARV+G FP   +++         +            +     GK A T F RI+ DG 
Sbjct: 188 YVARVIGEFPNSGEVI--------CDAPLLTVAPTLGLNRIHPDGKEASTVFKRIAFDGH 247

Query: 264 HSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTK-----------EASCRSV------ 323
            S+VLC+P+TGRTHQ+RVHLQY GYPIAND +Y  K           E S  SV      
Sbjct: 248 TSLVLCKPLTGRTHQLRVHLQYLGYPIANDPIYANKRVWGPSLGKNGEGSNESVIMRLSE 307

Query: 324 ---ERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEEGL 383
                T +          +TE +      ++ GE       C+ C          +E G+
Sbjct: 308 IGKTETASPLLQYEWYTNETEAEAKARRDKRLGE-LLTGKHCSECGTPLYSDPSPEELGI 367

Query: 384 WLHCVRYSGPGWIYECPYPEWA 385
           WLH ++Y    W Y+   P+WA
Sbjct: 368 WLHALKYESKDWSYKTQLPKWA 380

[Close]
BLAST of MC06g0032 vs. ExPASy Swiss-Prot
Match: Q149F1 (RNA pseudouridylate synthase domain-containing protein 2 OS=Mus musculus OX=10090 GN=Rpusd2 PE=1 SV=2)

HSP 1 Score: 232.3 bits (591), Expect = 9.5e-60
Identity = 137/411 (33.33%), Postives = 207/411 (50.36%), Query Frame = 0 

Query: 31  YILLNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGE 90
           Y    G R VRPYYF+F  + K RW G++++ +F+ EF+ +P  YY  AV+ GR+ ++ E
Sbjct: 146 YYFEGGLRKVRPYYFDFQTYCKGRWVGRSLLHVFSTEFRSQPLSYYEAAVRAGRLHLNEE 205

Query: 91  LV-PVSYIVRSSQKISHFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKN 150
            V  +S +++ +  + + +HRHEPPV A  + +L E  DV+ + KP+S+PVHPCG++R N
Sbjct: 206 PVQDLSIVLKDNDFLRNTVHRHEPPVTAEPIHLLAENNDVVVIDKPSSIPVHPCGRFRHN 265

Query: 151 TVVGILQAEHGLAPLFPIHRLDRLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVG 210
           TV+ IL  EH L  L P+HRLDRL SG+L+ A+ ++ ++   +Q+    ++K+Y+ RV G
Sbjct: 266 TVIFILGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSEKIHEQVRDRQLEKEYVCRVAG 325

Query: 211 VFPEKEQLVDVNINYNAREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCE 270
            FP+KE +         +E        V  C     +GK  +T F R+S +G  S+V C 
Sbjct: 326 EFPDKEVI--------CKEPILVVSYKVGVCR-VDPRGKPCETVFQRLSYNGRSSVVQCR 385

Query: 271 PITGRTHQIRVHLQYSGYPIANDALYLTKEAS---------CRSVERTNADRAAAH---- 330
           P+TGRTHQIRVHLQ+ G+PI ND +Y +              ++ E    D  A H    
Sbjct: 386 PLTGRTHQIRVHLQFLGHPILNDPIYNSTAWGPSRGQGGHIPKTDEELLRDLVAEHQAKE 445

Query: 331 --------------------CPARDTEEDC----------VDGCKE-------------K 385
                                P+ +  +D           +DG  E             K
Sbjct: 446 SLSMLDLCESDLTPGLIDSTAPSSELAKDSLEGLATAAQKIDGIAEAAPQHLDTPEKAAK 505

[Close]
BLAST of MC06g0032 vs. ExPASy Swiss-Prot
Match: Q8IZ73 (RNA pseudouridylate synthase domain-containing protein 2 OS=Homo sapiens OX=9606 GN=RPUSD2 PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.4e-58
Identity = 110/268 (41.04%), Postives = 168/268 (62.69%), Query Frame = 0 

Query: 29  SDYILLNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVD 88
           + Y    G R VRPYYF+F  + K RW G +++ +F+ EF+ +P  YY  AV+ GR+Q++
Sbjct: 131 TSYYFEGGLRKVRPYYFDFRTYCKGRWVGHSLLHVFSTEFRAQPLAYYEAAVRAGRLQLN 190

Query: 89  GELV-PVSYIVRSSQKISHFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYR 148
            + V  ++ +++ +  + + +HRHEPPV A  +++L E  DV+ V KP+S+PVHPCG++R
Sbjct: 191 EKPVQDLNIVLKDNDFLRNTVHRHEPPVTAEPIRLLAENEDVVVVDKPSSIPVHPCGRFR 250

Query: 149 KNTVVGILQAEHGLAPLFPIHRLDRLVSGLLILARNSSKADLFRQQIESGLVKKQYIARV 208
            NTV+ IL  EH L  L P+HRLDRL SG+L+ A+ ++ ++   +Q+    ++K+Y+ RV
Sbjct: 251 HNTVIFILGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSERIHEQVRDRQLEKEYVCRV 310

Query: 209 VGVFPEKEQLVDVNINYNAREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVL 268
            G FP +E           +E        V  C     +GK  +T F R+S +G  S+V 
Sbjct: 311 EGEFPTEE--------VTCKEPILVVSYKVGVCR-VDPRGKPCETVFQRLSYNGQSSVVR 370

Query: 269 CEPITGRTHQIRVHLQYSGYPIANDALY 296
           C P+TGRTHQIRVHLQ+ G+PI ND +Y
Sbjct: 371 CRPLTGRTHQIRVHLQFLGHPILNDPIY 389

[Close]
BLAST of MC06g0032 vs. NCBI nr
Match: XP_022134656.1 (RNA pseudouridine synthase 7 isoform X4 [Momordica charantia])

HSP 1 Score: 799 bits (2063), Expect = 3.73e-292
Identity = 385/386 (99.74%), Postives = 385/386 (99.74%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60
           MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI
Sbjct: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60

Query: 61  VDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDV 120
           VDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDV
Sbjct: 61  VDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDV 120

Query: 121 KILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLIL 180
           KILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLIL
Sbjct: 121 KILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLIL 180

Query: 181 ARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVDSC 240
           ARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAE MVDSC
Sbjct: 181 ARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAE-MVDSC 240

Query: 241 SDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEA 300
           SDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEA
Sbjct: 241 SDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEA 300

Query: 301 SCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEE 360
           SCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEE
Sbjct: 301 SCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEE 360

Query: 361 GLWLHCVRYSGPGWIYECPYPEWASF 386
           GLWLHCVRYSGPGWIYECPYPEWASF
Sbjct: 361 GLWLHCVRYSGPGWIYECPYPEWASF 385

[Close]
BLAST of MC06g0032 vs. NCBI nr
Match: XP_022134655.1 (RNA pseudouridine synthase 7 isoform X3 [Momordica charantia])

HSP 1 Score: 791 bits (2043), Expect = 5.89e-289
Identity = 385/395 (97.47%), Postives = 385/395 (97.47%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60
           MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI
Sbjct: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60

Query: 61  VDLFAEEFKGRPYEYY---------IDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRH 120
           VDLFAEEFKGRPYEYY         IDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRH
Sbjct: 61  VDLFAEEFKGRPYEYYVHFFLSVLLIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRH 120

Query: 121 EPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180
           EPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD
Sbjct: 121 EPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRS 240
           RLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRS
Sbjct: 181 RLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRS 240

Query: 241 TAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIAN 300
           TAE MVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIAN
Sbjct: 241 TAE-MVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIAN 300

Query: 301 DALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLG 360
           DALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLG
Sbjct: 301 DALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLG 360

Query: 361 PKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWASF 386
           PKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWASF
Sbjct: 361 PKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWASF 394

[Close]
BLAST of MC06g0032 vs. NCBI nr
Match: XP_008464394.1 (PREDICTED: RNA pseudouridine synthase 7 isoform X1 [Cucumis melo])

HSP 1 Score: 703 bits (1815), Expect = 3.07e-254
Identity = 342/390 (87.69%), Postives = 361/390 (92.56%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGE--IVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGK 60
           M SKRKREEE M++G   IVWQTPANPPQRSDYI  NGRRHVRPYYFEFIAHVKNRWAGK
Sbjct: 1   MKSKRKREEESMSMGMGGIVWQTPANPPQRSDYIFQNGRRHVRPYYFEFIAHVKNRWAGK 60

Query: 61  TIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMAC 120
           TIVDLFAEEFKGRPYEYY+DAVKCGRIQVDGE+VPVSYIV+SSQKISHFLHRHEPPVMA 
Sbjct: 61  TIVDLFAEEFKGRPYEYYVDAVKCGRIQVDGEIVPVSYIVKSSQKISHFLHRHEPPVMAW 120

Query: 121 DVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLL 180
            VK+LKEEA+VLTVCKPASVPVHPCGQYRKNTVVGIL+AEHGLAPLFPIHRLDRLVSGLL
Sbjct: 121 GVKVLKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLL 180

Query: 181 ILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVD 240
           ILARN+SKADLFRQQIESGLVKKQYIARVVGVFPE+EQ+VD NINYNAREGRSTAEQMVD
Sbjct: 181 ILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNAREGRSTAEQMVD 240

Query: 241 SCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTK 300
           S +D  TKGK AQTKFTRISS+G+HSIVLCEPITGRTHQIRVHLQY+GYPIANDALYLTK
Sbjct: 241 SSTDTPTKGKAAQTKFTRISSNGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTK 300

Query: 301 EASCRSVERTNADRAAA--HC-PARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGY 360
           EAS RSV++T ADRAAA   C PA D EEDC +  KE S EDFSID MCTNCPNL PKGY
Sbjct: 301 EASGRSVQKTTADRAAAISSCSPACDVEEDCANEWKENSSEDFSIDPMCTNCPNLAPKGY 360

Query: 361 EGDEEGLWLHCVRYSGPGWIYECPYPEWAS 385
           EG+EEGLWLHCVRYSGPGWIYECPYPEWAS
Sbjct: 361 EGEEEGLWLHCVRYSGPGWIYECPYPEWAS 390

[Close]
BLAST of MC06g0032 vs. NCBI nr
Match: XP_011650220.1 (RNA pseudouridine synthase 7 isoform X1 [Cucumis sativus])

HSP 1 Score: 697 bits (1800), Expect = 6.38e-252
Identity = 337/392 (85.97%), Postives = 357/392 (91.07%), Query Frame = 0 

Query: 1   MGSKRKREEEDM----AVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWA 60
           M SKRKREEE M     +G IVWQTP NPPQRSDYI  NGRRHVRPYYFEFIAHVKNRWA
Sbjct: 1   MRSKRKREEESMRMGMGMGGIVWQTPDNPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWA 60

Query: 61  GKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVM 120
           GKTIVDLFAEEFKGRPYEYY+DAV+CGRIQVDGE+VPVSYIV+SSQKISHFLHRHEPPVM
Sbjct: 61  GKTIVDLFAEEFKGRPYEYYVDAVECGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVM 120

Query: 121 ACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSG 180
           A  VK+LKEE +VLTVCKPASVPVHPCGQYRKNTVVGIL+AEHGLAPLFPIHRLDRLVSG
Sbjct: 121 AWGVKVLKEEEEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSG 180

Query: 181 LLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQM 240
           LLILARN+SKADLFRQQIESGLVKKQYIARVVGVFPE+EQ+VD NINYN REGRSTAEQM
Sbjct: 181 LLILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNPREGRSTAEQM 240

Query: 241 VDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYL 300
           VDS +D RTKGK AQTKFTRISSDG+HSIV CEPITGRTHQIRVHLQY+GYPIANDALYL
Sbjct: 241 VDSSTDTRTKGKAAQTKFTRISSDGVHSIVSCEPITGRTHQIRVHLQYTGYPIANDALYL 300

Query: 301 TKEASCRSVERTNADRAAA--HC-PARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPK 360
           TKEAS RSV++T ADRAAA   C PARD +EDC + CKE   EDF ID MCTNCPNL PK
Sbjct: 301 TKEASGRSVQKTTADRAAAISFCSPARDVQEDCANACKENWSEDFGIDPMCTNCPNLAPK 360

Query: 361 GYEGDEEGLWLHCVRYSGPGWIYECPYPEWAS 385
           GYEG+EEGLWLHCV+YSGPGWIYECPYPEWAS
Sbjct: 361 GYEGEEEGLWLHCVQYSGPGWIYECPYPEWAS 392

[Close]
BLAST of MC06g0032 vs. NCBI nr
Match: XP_008464395.1 (PREDICTED: RNA pseudouridine synthase 7 isoform X2 [Cucumis melo])

HSP 1 Score: 697 bits (1798), Expect = 1.15e-251
Identity = 341/390 (87.44%), Postives = 360/390 (92.31%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGE--IVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGK 60
           M SKRKREEE M++G   IVWQTPANPPQRSDYI  NGRRHVRPYYFEFIAHVKNRWAGK
Sbjct: 1   MKSKRKREEESMSMGMGGIVWQTPANPPQRSDYIFQNGRRHVRPYYFEFIAHVKNRWAGK 60

Query: 61  TIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMAC 120
           TIVDLFAEEFKGRPYEYY+DAVKCGRIQVDGE+VPVSYIV+SSQKISHFLHRHEPPVMA 
Sbjct: 61  TIVDLFAEEFKGRPYEYYVDAVKCGRIQVDGEIVPVSYIVKSSQKISHFLHRHEPPVMAW 120

Query: 121 DVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLL 180
            VK+LKEEA+VLTVCKPASVPVHPCGQYRKNTVVGIL+AEHGLAPLFPIHRLDRLVSGLL
Sbjct: 121 GVKVLKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLL 180

Query: 181 ILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVD 240
           ILARN+SKADLFRQQIESGLVKKQYIARVVGVFPE+EQ+VD NINYNAREGRSTAE MVD
Sbjct: 181 ILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNAREGRSTAE-MVD 240

Query: 241 SCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTK 300
           S +D  TKGK AQTKFTRISS+G+HSIVLCEPITGRTHQIRVHLQY+GYPIANDALYLTK
Sbjct: 241 SSTDTPTKGKAAQTKFTRISSNGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTK 300

Query: 301 EASCRSVERTNADRAAA--HC-PARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGY 360
           EAS RSV++T ADRAAA   C PA D EEDC +  KE S EDFSID MCTNCPNL PKGY
Sbjct: 301 EASGRSVQKTTADRAAAISSCSPACDVEEDCANEWKENSSEDFSIDPMCTNCPNLAPKGY 360

Query: 361 EGDEEGLWLHCVRYSGPGWIYECPYPEWAS 385
           EG+EEGLWLHCVRYSGPGWIYECPYPEWAS
Sbjct: 361 EGEEEGLWLHCVRYSGPGWIYECPYPEWAS 389

[Close]
BLAST of MC06g0032 vs. ExPASy TrEMBL
Match: A0A6J1BYF2 (Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111006876 PE=3 SV=1)

HSP 1 Score: 799 bits (2063), Expect = 1.81e-292
Identity = 385/386 (99.74%), Postives = 385/386 (99.74%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60
           MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI
Sbjct: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60

Query: 61  VDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDV 120
           VDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDV
Sbjct: 61  VDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMACDV 120

Query: 121 KILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLIL 180
           KILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLIL
Sbjct: 121 KILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLLIL 180

Query: 181 ARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVDSC 240
           ARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAE MVDSC
Sbjct: 181 ARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAE-MVDSC 240

Query: 241 SDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEA 300
           SDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEA
Sbjct: 241 SDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTKEA 300

Query: 301 SCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEE 360
           SCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEE
Sbjct: 301 SCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGYEGDEE 360

Query: 361 GLWLHCVRYSGPGWIYECPYPEWASF 386
           GLWLHCVRYSGPGWIYECPYPEWASF
Sbjct: 361 GLWLHCVRYSGPGWIYECPYPEWASF 385

[Close]
BLAST of MC06g0032 vs. ExPASy TrEMBL
Match: A0A6J1BYX0 (RNA pseudouridine synthase 7 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111006876 PE=4 SV=1)

HSP 1 Score: 791 bits (2043), Expect = 2.85e-289
Identity = 385/395 (97.47%), Postives = 385/395 (97.47%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60
           MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI
Sbjct: 1   MGSKRKREEEDMAVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGKTI 60

Query: 61  VDLFAEEFKGRPYEYY---------IDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRH 120
           VDLFAEEFKGRPYEYY         IDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRH
Sbjct: 61  VDLFAEEFKGRPYEYYVHFFLSVLLIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRH 120

Query: 121 EPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180
           EPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD
Sbjct: 121 EPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRS 240
           RLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRS
Sbjct: 181 RLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRS 240

Query: 241 TAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIAN 300
           TAE MVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIAN
Sbjct: 241 TAE-MVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIAN 300

Query: 301 DALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLG 360
           DALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLG
Sbjct: 301 DALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLG 360

Query: 361 PKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWASF 386
           PKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWASF
Sbjct: 361 PKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWASF 394

[Close]
BLAST of MC06g0032 vs. ExPASy TrEMBL
Match: A0A1S3CLH7 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1)

HSP 1 Score: 703 bits (1815), Expect = 1.48e-254
Identity = 342/390 (87.69%), Postives = 361/390 (92.56%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGE--IVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGK 60
           M SKRKREEE M++G   IVWQTPANPPQRSDYI  NGRRHVRPYYFEFIAHVKNRWAGK
Sbjct: 1   MKSKRKREEESMSMGMGGIVWQTPANPPQRSDYIFQNGRRHVRPYYFEFIAHVKNRWAGK 60

Query: 61  TIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMAC 120
           TIVDLFAEEFKGRPYEYY+DAVKCGRIQVDGE+VPVSYIV+SSQKISHFLHRHEPPVMA 
Sbjct: 61  TIVDLFAEEFKGRPYEYYVDAVKCGRIQVDGEIVPVSYIVKSSQKISHFLHRHEPPVMAW 120

Query: 121 DVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLL 180
            VK+LKEEA+VLTVCKPASVPVHPCGQYRKNTVVGIL+AEHGLAPLFPIHRLDRLVSGLL
Sbjct: 121 GVKVLKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLL 180

Query: 181 ILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVD 240
           ILARN+SKADLFRQQIESGLVKKQYIARVVGVFPE+EQ+VD NINYNAREGRSTAEQMVD
Sbjct: 181 ILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNAREGRSTAEQMVD 240

Query: 241 SCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTK 300
           S +D  TKGK AQTKFTRISS+G+HSIVLCEPITGRTHQIRVHLQY+GYPIANDALYLTK
Sbjct: 241 SSTDTPTKGKAAQTKFTRISSNGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTK 300

Query: 301 EASCRSVERTNADRAAA--HC-PARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGY 360
           EAS RSV++T ADRAAA   C PA D EEDC +  KE S EDFSID MCTNCPNL PKGY
Sbjct: 301 EASGRSVQKTTADRAAAISSCSPACDVEEDCANEWKENSSEDFSIDPMCTNCPNLAPKGY 360

Query: 361 EGDEEGLWLHCVRYSGPGWIYECPYPEWAS 385
           EG+EEGLWLHCVRYSGPGWIYECPYPEWAS
Sbjct: 361 EGEEEGLWLHCVRYSGPGWIYECPYPEWAS 390

[Close]
BLAST of MC06g0032 vs. ExPASy TrEMBL
Match: A0A1S3CLD1 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1)

HSP 1 Score: 697 bits (1798), Expect = 5.57e-252
Identity = 341/390 (87.44%), Postives = 360/390 (92.31%), Query Frame = 0 

Query: 1   MGSKRKREEEDMAVGE--IVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWAGK 60
           M SKRKREEE M++G   IVWQTPANPPQRSDYI  NGRRHVRPYYFEFIAHVKNRWAGK
Sbjct: 1   MKSKRKREEESMSMGMGGIVWQTPANPPQRSDYIFQNGRRHVRPYYFEFIAHVKNRWAGK 60

Query: 61  TIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVMAC 120
           TIVDLFAEEFKGRPYEYY+DAVKCGRIQVDGE+VPVSYIV+SSQKISHFLHRHEPPVMA 
Sbjct: 61  TIVDLFAEEFKGRPYEYYVDAVKCGRIQVDGEIVPVSYIVKSSQKISHFLHRHEPPVMAW 120

Query: 121 DVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSGLL 180
            VK+LKEEA+VLTVCKPASVPVHPCGQYRKNTVVGIL+AEHGLAPLFPIHRLDRLVSGLL
Sbjct: 121 GVKVLKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLL 180

Query: 181 ILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQMVD 240
           ILARN+SKADLFRQQIESGLVKKQYIARVVGVFPE+EQ+VD NINYNAREGRSTAE MVD
Sbjct: 181 ILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNAREGRSTAE-MVD 240

Query: 241 SCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYLTK 300
           S +D  TKGK AQTKFTRISS+G+HSIVLCEPITGRTHQIRVHLQY+GYPIANDALYLTK
Sbjct: 241 SSTDTPTKGKAAQTKFTRISSNGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTK 300

Query: 301 EASCRSVERTNADRAAA--HC-PARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPKGY 360
           EAS RSV++T ADRAAA   C PA D EEDC +  KE S EDFSID MCTNCPNL PKGY
Sbjct: 301 EASGRSVQKTTADRAAAISSCSPACDVEEDCANEWKENSSEDFSIDPMCTNCPNLAPKGY 360

Query: 361 EGDEEGLWLHCVRYSGPGWIYECPYPEWAS 385
           EG+EEGLWLHCVRYSGPGWIYECPYPEWAS
Sbjct: 361 EGEEEGLWLHCVRYSGPGWIYECPYPEWAS 389

[Close]
BLAST of MC06g0032 vs. ExPASy TrEMBL
Match: A0A0A0LR57 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G001310 PE=3 SV=1)

HSP 1 Score: 691 bits (1783), Expect = 1.16e-249
Identity = 336/392 (85.71%), Postives = 356/392 (90.82%), Query Frame = 0 

Query: 1   MGSKRKREEEDM----AVGEIVWQTPANPPQRSDYILLNGRRHVRPYYFEFIAHVKNRWA 60
           M SKRKREEE M     +G IVWQTP NPPQRSDYI  NGRRHVRPYYFEFIAHVKNRWA
Sbjct: 1   MRSKRKREEESMRMGMGMGGIVWQTPDNPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWA 60

Query: 61  GKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKISHFLHRHEPPVM 120
           GKTIVDLFAEEFKGRPYEYY+DAV+CGRIQVDGE+VPVSYIV+SSQKISHFLHRHEPPVM
Sbjct: 61  GKTIVDLFAEEFKGRPYEYYVDAVECGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVM 120

Query: 121 ACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDRLVSG 180
           A  VK+LKEE +VLTVCKPASVPVHPCGQYRKNTVVGIL+AEHGLAPLFPIHRLDRLVSG
Sbjct: 121 AWGVKVLKEEEEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSG 180

Query: 181 LLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQM 240
           LLILARN+SKADLFRQQIESGLVKKQYIARVVGVFPE+EQ+VD NINYN REGRSTAE M
Sbjct: 181 LLILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNPREGRSTAE-M 240

Query: 241 VDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYSGYPIANDALYL 300
           VDS +D RTKGK AQTKFTRISSDG+HSIV CEPITGRTHQIRVHLQY+GYPIANDALYL
Sbjct: 241 VDSSTDTRTKGKAAQTKFTRISSDGVHSIVSCEPITGRTHQIRVHLQYTGYPIANDALYL 300

Query: 301 TKEASCRSVERTNADRAAA--HC-PARDTEEDCVDGCKEKSGEDFSIDSMCTNCPNLGPK 360
           TKEAS RSV++T ADRAAA   C PARD +EDC + CKE   EDF ID MCTNCPNL PK
Sbjct: 301 TKEASGRSVQKTTADRAAAISFCSPARDVQEDCANACKENWSEDFGIDPMCTNCPNLAPK 360

Query: 361 GYEGDEEGLWLHCVRYSGPGWIYECPYPEWAS 385
           GYEG+EEGLWLHCV+YSGPGWIYECPYPEWAS
Sbjct: 361 GYEGEEEGLWLHCVQYSGPGWIYECPYPEWAS 391

[Close]
BLAST of MC06g0032 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 509.6 bits (1311), Expect = 2.2e-144
Identity = 253/400 (63.25%), Postives = 296/400 (74.00%), Query Frame = 0 

Query: 4   KRKREEEDMAVG------------------EIVWQTPANPPQRSDYILLNGRRHVRPYYF 63
           KRK++E+D   G                  +++ Q+ +   Q+ DYI   GRRHVRPYYF
Sbjct: 2   KRKQQEDDNDDGVEKAVSPVLATTSNTMSTDLLLQSSSKLSQKQDYIFHGGRRHVRPYYF 61

Query: 64  EFIAHVKNRWAGKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKIS 123
           EFI+HV  RW GKTIVDLFA+EFKGRP +YY+ AVK GRI+VDGE+VPVSYIV+SSQKI+
Sbjct: 62  EFISHVNKRWTGKTIVDLFADEFKGRPRDYYVGAVKSGRIKVDGEIVPVSYIVKSSQKIT 121

Query: 124 HFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLF 183
           HFLHRHEPPVM  DV IL +E DV+TVCKPASVPVHPCGQYRKNT+VGIL AEH L  LF
Sbjct: 122 HFLHRHEPPVMIDDVVILHQEPDVVTVCKPASVPVHPCGQYRKNTIVGILDAEHDLGTLF 181

Query: 184 PIHRLDRLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYN 243
           PIHRLDRLVSGLLI+AR ++KAD FRQQIE G+VKK+YIA+V+GVFPE E +VD NINYN
Sbjct: 182 PIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANINYN 241

Query: 244 AREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYS 303
             EGRSTAE    S  D + KGK A TKFTRI ++G HS+V CEP+TGRTHQIRVHLQY+
Sbjct: 242 GSEGRSTAEDANSSGDDKKVKGKPACTKFTRIDTNGTHSLVSCEPVTGRTHQIRVHLQYT 301

Query: 304 GYPIANDALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCT 363
           G+PIAND LYL +     ++E   A R  A      + +D V      S EDFSID MCT
Sbjct: 302 GHPIANDPLYLNQHID--NLETYIAKRIDAGERKIVSPDDYV-----YSSEDFSIDPMCT 361

Query: 364 NCPNLGPKGYEGDEEGLWLHCVRYSGPGWIYECPYPEWAS 386
           NCP L P+GYE  +E LWLHCV+Y G GW YECPYP WAS
Sbjct: 362 NCPKLIPQGYEEHDEALWLHCVQYCGTGWEYECPYPSWAS 394

[Close]
BLAST of MC06g0032 vs. TAIR 10
Match: AT5G51140.2 (Pseudouridine synthase family protein )

HSP 1 Score: 490.3 bits (1261), Expect = 1.4e-138
Identity = 246/392 (62.76%), Postives = 289/392 (73.72%), Query Frame = 0 

Query: 4   KRKREEEDMAVG------------------EIVWQTPANPPQRSDYILLNGRRHVRPYYF 63
           KRK++E+D   G                  +++ Q+ +   Q+ DYI   GRRHVRPYYF
Sbjct: 2   KRKQQEDDNDDGVEKAVSPVLATTSNTMSTDLLLQSSSKLSQKQDYIFHGGRRHVRPYYF 61

Query: 64  EFIAHVKNRWAGKTIVDLFAEEFKGRPYEYYIDAVKCGRIQVDGELVPVSYIVRSSQKIS 123
           EFI+HV  RW GKTIVDLFA+EFKGRP +YY+ AVK GRI+VDGE+VPVSYIV+SSQKI+
Sbjct: 62  EFISHVNKRWTGKTIVDLFADEFKGRPRDYYVGAVKSGRIKVDGEIVPVSYIVKSSQKIT 121

Query: 124 HFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLF 183
           HFLHRHEPPVM  DV IL +E DV+TVCKPASVPVHPCGQYRKNT+VGIL AEH L  LF
Sbjct: 122 HFLHRHEPPVMIDDVVILHQEPDVVTVCKPASVPVHPCGQYRKNTIVGILDAEHDLGTLF 181

Query: 184 PIHRLDRLVSGLLILARNSSKADLFRQQIESGLVKKQYIARVVGVFPEKEQLVDVNINYN 243
           PIHRLDRLVSGLLI+AR ++KAD FRQQIE G+VKK+YIA+V+GVFPE E +VD NINYN
Sbjct: 182 PIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANINYN 241

Query: 244 AREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIHSIVLCEPITGRTHQIRVHLQYS 303
             EGRSTAE    S  D + KGK A TKFTRI ++G HS+V CEP+TGRTHQIRVHLQY+
Sbjct: 242 GSEGRSTAEDANSSGDDKKVKGKPACTKFTRIDTNGTHSLVSCEPVTGRTHQIRVHLQYT 301

Query: 304 GYPIANDALYLTKEASCRSVERTNADRAAAHCPARDTEEDCVDGCKEKSGEDFSIDSMCT 363
           G+PIAND LYL +     ++E   A R  A      + +D V      S EDFSID MCT
Sbjct: 302 GHPIANDPLYLNQHID--NLETYIAKRIDAGERKIVSPDDYV-----YSSEDFSIDPMCT 361

Query: 364 NCPNLGPKGYEGDEEGLWLHCVRYSGPGWIYE 378
           NCP L P+GYE  +E LWLHCV+Y G GW YE
Sbjct: 362 NCPKLIPQGYEEHDEALWLHCVQYCGTGWEYE 386

[Close]
BLAST of MC06g0032 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 80.9 bits (198), Expect = 2.5e-15
Identity = 72/269 (26.77%), Postives = 124/269 (46.10%), Query Frame = 0 

Query: 59  TIVDLFAEEFK-GRPYEYYIDAVKCGRIQVDGELV-PVSYIVRSSQKISHFLHRHEPPVM 118
           T+ + +  ++K   P   +I  ++ G+IQ+DGE+V   + ++RS  K+ +     + P  
Sbjct: 34  TVSEFYFTKYKSSAPLLGWIQRIQNGQIQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDT 93

Query: 119 ACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLF----------P 178
              +++L E+ D++ + KP+ + V P G +++ TV+  LQ   G    +          P
Sbjct: 94  PYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVP 153

Query: 179 IHRLDRLVSGLLILARNS-SKADL---------------FRQQIESG-LVKKQYIARVVG 238
           +HRL R  SG+L+ A+   +K  L                 Q+  +G  + K Y A   G
Sbjct: 154 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 213

Query: 239 VFPEKEQLVDVNINYNAREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIH--SIVL 297
           +  E E ++   I      G      +      A  +GK A +K   +  D     S+V 
Sbjct: 214 IVEEDEVVIKQPIGVVRYPG------VAQGLYVASPEGKPAFSKVFVLERDREKNCSLVK 273

[Close]
BLAST of MC06g0032 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 73.9 bits (180), Expect = 3.1e-13
Identity = 69/262 (26.34%), Postives = 120/262 (45.80%), Query Frame = 0 

Query: 59  TIVDLFAEEFK-GRPYEYYIDAVKCGRIQVDGELV-PVSYIVRSSQKISHFLHRHEPPVM 118
           T+ + +  ++K   P   +I  ++ G+IQ+DGE+V   + ++RS  K+ +     + P  
Sbjct: 34  TVSEFYFTKYKSSAPLLGWIQRIQNGQIQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDT 93

Query: 119 ACDVKILKEEADVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLF----------P 178
              +++L E+ D++ + KP+ + V P G +++ TV+  LQ   G    +          P
Sbjct: 94  PYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVP 153

Query: 179 IHRLDRLVSGLLILARNS-SKADL---------------FRQQIESG-LVKKQYIARVVG 238
           +HRL R  SG+L+ A+   +K  L                 Q+  +G  + K Y A   G
Sbjct: 154 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 213

Query: 239 VFPEKEQLVDVNINYNAREGRSTAEQMVDSCSDARTKGKTAQTKFTRISSDGIH--SIVL 290
           +  E E ++   I      G      +      A  +GK A +K   +  D     S+V 
Sbjct: 214 IVEEDEVVIKQPIGVVRYPG------VAQGLYVASPEGKPAFSKVFVLERDREKNCSLVK 273

[Close]
BLAST of MC06g0032 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 2.2e-11
Identity = 67/257 (26.07%), Postives = 114/257 (44.36%), Query Frame = 0 

Query: 85  IQVDGELV-PVSYIVRSSQKISHFLHRHEPPVMACDVKILKEEADVLTVCKPASVPVHPC 144
           IQ+DGE+V   + ++RS  K+ +     + P     +++L E+ D++ + KP+ + V P 
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQVLPG 150

Query: 145 GQYRKNTVVGILQAEHGLAPLF----------PIHRLDRLVSGLLILARNS-SKADL--- 204
           G +++ TV+  LQ   G    +          P+HRL R  SG+L+ A+   +K  L   
Sbjct: 151 GLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLGRGTSGILLCAKTKLAKTKLAAY 210

Query: 205 ------------FRQQIESG-LVKKQYIARVVGVFPEKEQLVDVNINYNAREGRSTAEQM 264
                         Q+  +G  + K Y A   G+  E E ++   I      G      +
Sbjct: 211 FAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEEDEVVIKQPIGVVRYPG------V 270

Query: 265 VDSCSDARTKGKTAQTKFTRISSDGIH--SIVLCEPITGRTHQIRVHLQYSGYPIANDAL 312
                 A  +GK A +K   +  D     S+V  E  +GR HQIR+HL Y G+P+    +
Sbjct: 271 AQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVEMTM 330

The following BLAST results are available for this feature:
BLAST of MC06g0032 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q0E0Y36.9e-15167.45RNA pseudouridine synthase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g05... [more]
Q9LU603.1e-14363.25RNA pseudouridine synthase 7 OS=Arabidopsis thaliana OX=3702 GN=At5g51140 PE=2 S... [more]
Q097091.9e-6034.82Uncharacterized protein C18B11.02c OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Q149F19.5e-6033.33RNA pseudouridylate synthase domain-containing protein 2 OS=Mus musculus OX=1009... [more]
Q8IZ731.4e-5841.04RNA pseudouridylate synthase domain-containing protein 2 OS=Homo sapiens OX=9606... [more]
BLAST of MC06g0032 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022134656.13.73e-29299.74RNA pseudouridine synthase 7 isoform X4 [Momordica charantia][more]
XP_022134655.15.89e-28997.47RNA pseudouridine synthase 7 isoform X3 [Momordica charantia][more]
XP_008464394.13.07e-25487.69PREDICTED: RNA pseudouridine synthase 7 isoform X1 [Cucumis melo][more]
XP_011650220.16.38e-25285.97RNA pseudouridine synthase 7 isoform X1 [Cucumis sativus][more]
XP_008464395.11.15e-25187.44PREDICTED: RNA pseudouridine synthase 7 isoform X2 [Cucumis melo][more]
BLAST of MC06g0032 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BYF21.81e-29299.74Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111006876 PE=3 SV=1[more]
A0A6J1BYX02.85e-28997.47RNA pseudouridine synthase 7 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3CLH71.48e-25487.69Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1[more]
A0A1S3CLD15.57e-25287.44Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1[more]
A0A0A0LR571.16e-24985.71Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G001310 PE=3 SV=1[more]
BLAST of MC06g0032 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G51140.12.2e-14463.25Pseudouridine synthase family protein [more]
AT5G51140.21.4e-13862.76Pseudouridine synthase family protein [more]
AT3G52260.22.5e-1526.77Pseudouridine synthase family protein [more]
AT3G52260.13.1e-1326.34Pseudouridine synthase family protein [more]
AT3G52260.32.2e-1126.07Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006225Pseudouridine synthase, RluC/RluDTIGRFAMTIGR00005TIGR00005coord: 54..314
e-value: 2.2E-59
score: 199.2
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 129..283
e-value: 5.5E-30
score: 104.6
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 113..330
e-value: 1.1E-48
score: 167.6
NoneNo IPR availablePANTHERPTHR21600:SF62PSEUDOURIDINE SYNTHASEcoord: 12..385
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 12..385
NoneNo IPR availableCDDcd02557PseudoU_synth_ScRIB2coord: 105..385
e-value: 1.16267E-100
score: 295.308
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 167..181
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 112..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0032.1MC06g0032.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding