MC06g0029 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0029
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Mitochondrial import inner membrane translocase subunit
Location	MC06: 194194 .. 194377 (+)
RNA-Seq Expression	MC06g0029
Synteny	MC06g0029

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGAGAAGAAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTTACCAGTGTGTGCTGGGACAAAGTGTATCACTGGTTCGCCAGGGAGTAAGTTCAGCTCCAGGGAATCCAACTGTCTATCAAACTGCGCCCAACGCTATATGGATATGAGCATCATCATTATGAAACGGTTCCAAAACAGT

mRNA sequence

GAAGAGAAGAAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTTACCAGTGTGTGCTGGGACTGTATCACTGGTTCGCCAGGGAGTAAGTTCAGCTCCAGGGAATCCAACTGTCTATCAAACTGCGCCCAACGCTATATGGATATGAGCATCATCATTATGAAACGGTTCCAAAACAGT

Coding sequence (CDS)

GAAGAGAAGAAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTTACCAGTGTGTGCTGGGACTGTATCACTGGTTCGCCAGGGAGTAAGTTCAGCTCCAGGGAATCCAACTGTCTATCAAACTGCGCCCAACGCTATATGGATATGAGCATCATCATTATGAAACGGTTCCAAAACAGT

Protein sequence

EEKKRAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQNS

Homology

[Close]

BLAST of MC06g0029 vs. ExPASy Swiss-Prot
Match: Q9XGY4 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thaliana OX=3702 GN=TIM8 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.8e-19
Identity = 45/60 (75.00%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          +EK+RAMV EMV+K+TSVCWD CIT +PGSKFSS ES+CL++CAQRYMDMS+IIMKRF +
Sbjct: 17 QEKERAMVNEMVSKMTSVCWDKCITSAPGSKFSSSESSCLTHCAQRYMDMSMIIMKRFNS 76

[Close]

BLAST of MC06g0029 vs. ExPASy Swiss-Prot
Match: P0CR95 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=TIM8 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.2e-07
Identity = 23/59 (38.98%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQ 59
          +E+ +A +   + +LT+ CW+ CITG   SKFS  E+ CL NC  R++D S+ I+++ +
Sbjct: 24 QEQAKAKLQASIHELTNTCWNTCITGGISSKFSKSEAQCLENCVDRFLDSSLYIVRQIE 82

[Close]

BLAST of MC06g0029 vs. ExPASy Swiss-Prot
Match: P0CR94 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=TIM8 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.2e-07
Identity = 23/59 (38.98%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQ 59
          +E+ +A +   + +LT+ CW+ CITG   SKFS  E+ CL NC  R++D S+ I+++ +
Sbjct: 24 QEQAKAKLQASIHELTNTCWNTCITGGISSKFSKSEAQCLENCVDRFLDSSLYIVRQIE 82

[Close]

BLAST of MC06g0029 vs. ExPASy Swiss-Prot
Match: Q9Y1A3 (Mitochondrial import inner membrane translocase subunit Tim8 OS=Drosophila melanogaster OX=7227 GN=Tim8 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.6e-06
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 0


Query: 2  EKKRAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRF 58
          EK++A V   + +   +CW+   G P +K       CLSNC  R++D S++I +RF
Sbjct: 21 EKQKAQVNAQIHEFNEICWEKCIGKPSTKLDHATETCLSNCVDRFIDTSLLITQRF 76

[Close]

BLAST of MC06g0029 vs. ExPASy Swiss-Prot
Match: P62077 (Mitochondrial import inner membrane translocase subunit Tim8 B OS=Mus musculus OX=10090 GN=Timm8b PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.4e-05
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 0


Query: 2  EKKRAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRF 58
          E+++A     V     +CWD     PGS+  SR  NCLS+C  R++D ++ I  RF
Sbjct: 19 EQQKAQFTAQVHHFMELCWDKCVEKPGSRLDSRTENCLSSCVDRFIDTTLAITGRF 74

[Close]

BLAST of MC06g0029 vs. NCBI nr
Match: XP_022135207.1 (mitochondrial import inner membrane translocase subunit TIM8 [Momordica charantia])

HSP 1 Score: 116 bits (290), Expect = 2.48e-32
Identity = 58/61 (95.08%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEK+RAMVAEMVAKLTSVCWD CITGSPGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. NCBI nr
Match: XP_008464391.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis melo] >XP_011649996.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucumis sativus] >XP_022921432.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita moschata] >XP_022924732.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita moschata] >XP_022974369.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita maxima] >XP_022988609.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita maxima] >XP_023516926.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita pepo subsp. pepo] >XP_023529319.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita pepo subsp. pepo] >XP_038878330.1 mitochondrial import inner membrane translocase subunit TIM8 [Benincasa hispida] >KGN63456.1 hypothetical protein Csa_014027 [Cucumis sativus])

HSP 1 Score: 115 bits (287), Expect = 7.11e-32
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEK+RAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. NCBI nr
Match: KAG7023044.1 (Mitochondrial import inner membrane translocase subunit TIM8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 110 bits (275), Expect = 1.76e-29
Identity = 54/60 (90.00%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 59
          EEK+RAMVAEMVAKLTSVCW  C+TG+PGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWTKCVTGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. NCBI nr
Match: XP_028764582.1 (mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba] >XP_028765220.1 mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba])

HSP 1 Score: 104 bits (259), Expect = 1.37e-27
Identity = 50/60 (83.33%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 59
          +EK+RAMV EMVAKLTS CWD CITG+PGSKFSS ES CLSNCAQRYMD+SI+IMKRFQN
Sbjct: 17 QEKERAMVNEMVAKLTSACWDKCITGTPGSKFSSSESTCLSNCAQRYMDLSILIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. NCBI nr
Match: XP_018830814.1 (mitochondrial import inner membrane translocase subunit TIM8 [Juglans regia] >XP_040988263.1 mitochondrial import inner membrane translocase subunit TIM8 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 103 bits (258), Expect = 1.95e-27
Identity = 50/60 (83.33%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 59
          +EK+RAMV EMVAKLT+VCWD CITG+PGSKFSS ES+CLSNCA+RYMDMSIII+KRFQN
Sbjct: 17 QEKERAMVNEMVAKLTNVCWDKCITGTPGSKFSSSESSCLSNCARRYMDMSIIIVKRFQN 76

[Close]

BLAST of MC06g0029 vs. ExPASy TrEMBL
Match: A0A6J1C212 (Mitochondrial import inner membrane translocase subunit OS=Momordica charantia OX=3673 GN=LOC111007226 PE=3 SV=1)

HSP 1 Score: 116 bits (290), Expect = 1.20e-32
Identity = 58/61 (95.08%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEK+RAMVAEMVAKLTSVCWD CITGSPGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. ExPASy TrEMBL
Match: A0A6J1IHF0 (Mitochondrial import inner membrane translocase subunit OS=Cucurbita maxima OX=3661 GN=LOC111472993 PE=3 SV=1)

HSP 1 Score: 115 bits (287), Expect = 3.44e-32
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEK+RAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. ExPASy TrEMBL
Match: A0A6J1E0G1 (Mitochondrial import inner membrane translocase subunit OS=Cucurbita moschata OX=3662 GN=LOC111429714 PE=3 SV=1)

HSP 1 Score: 115 bits (287), Expect = 3.44e-32
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEK+RAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. ExPASy TrEMBL
Match: A0A0A0LTY7 (Mitochondrial import inner membrane translocase subunit OS=Cucumis sativus OX=3659 GN=Csa_1G001280 PE=3 SV=1)

HSP 1 Score: 115 bits (287), Expect = 3.44e-32
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEK+RAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. ExPASy TrEMBL
Match: A0A1S3CLD0 (Mitochondrial import inner membrane translocase subunit OS=Cucumis melo OX=3656 GN=LOC103502294 PE=3 SV=1)

HSP 1 Score: 115 bits (287), Expect = 3.44e-32
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEK+RAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MC06g0029 vs. TAIR 10
Match: AT5G50810.1 (translocase inner membrane subunit 8 )

HSP 1 Score: 95.1 bits (235), Expect = 2.0e-20
Identity = 45/60 (75.00%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 1  EEKKRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMKRFQN 60
          +EK+RAMV EMV+K+TSVCWD CIT +PGSKFSS ES+CL++CAQRYMDMS+IIMKRF +
Sbjct: 17 QEKERAMVNEMVSKMTSVCWDKCITSAPGSKFSSSESSCLTHCAQRYMDMSMIIMKRFNS 76

[Close]

BLAST of MC06g0029 vs. TAIR 10
Match: AT1G61570.1 (translocase of the inner mitochondrial membrane 13 )

HSP 1 Score: 42.7 bits (99), Expect = 1.2e-04
Identity = 18/51 (35.29%), Postives = 29/51 (56.86%), Query Frame = 0


Query: 5  RAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIIIMK 56
          +A   E++  L + C+D     PGS     ES+C+S C +RYM+ + II +
Sbjct: 31 QAYAEELIETLRTKCFDKCVTKPGSSLGGSESSCISRCVERYMEATAIISR 81

The following BLAST results are available for this feature:

BLAST of MC06g0029 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGY4	2.8e-19	75.00	Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thal...	[more]
P0CR95	3.2e-07	38.98	Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neo...	[more]
P0CR94	3.2e-07	38.98	Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neo...	[more]
Q9Y1A3	3.6e-06	35.71	Mitochondrial import inner membrane translocase subunit Tim8 OS=Drosophila melan...	[more]
P62077	1.4e-05	37.50	Mitochondrial import inner membrane translocase subunit Tim8 B OS=Mus musculus O...	[more]

BLAST of MC06g0029 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135207.1	2.48e-32	95.08	mitochondrial import inner membrane translocase subunit TIM8 [Momordica charanti...	[more]
XP_008464391.1	7.11e-32	93.44	PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis...	[more]
KAG7023044.1	1.76e-29	90.00	Mitochondrial import inner membrane translocase subunit TIM8, partial [Cucurbita...	[more]
XP_028764582.1	1.37e-27	83.33	mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba...	[more]
XP_018830814.1	1.95e-27	83.33	mitochondrial import inner membrane translocase subunit TIM8 [Juglans regia] >XP...	[more]

BLAST of MC06g0029 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C212	1.20e-32	95.08	Mitochondrial import inner membrane translocase subunit OS=Momordica charantia O...	[more]
A0A6J1IHF0	3.44e-32	93.44	Mitochondrial import inner membrane translocase subunit OS=Cucurbita maxima OX=3...	[more]
A0A6J1E0G1	3.44e-32	93.44	Mitochondrial import inner membrane translocase subunit OS=Cucurbita moschata OX...	[more]
A0A0A0LTY7	3.44e-32	93.44	Mitochondrial import inner membrane translocase subunit OS=Cucumis sativus OX=36...	[more]
A0A1S3CLD0	3.44e-32	93.44	Mitochondrial import inner membrane translocase subunit OS=Cucumis melo OX=3656 ...	[more]

BLAST of MC06g0029 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50810.1	2.0e-20	75.00	translocase inner membrane subunit 8	[more]
AT1G61570.1	1.2e-04	35.29	translocase of the inner mitochondrial membrane 13	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035427	Tim10-like domain superfamily	GENE3D	1.10.287.810	Mitochondrial import inner membrane translocase subunit tim13 like domains	coord: 1..60 e-value: 7.0E-19 score: 69.0
IPR035427	Tim10-like domain superfamily	SUPERFAMILY	144122	Tim10-like	coord: 6..59
IPR004217	Tim10-like	PFAM	PF02953	zf-Tim10_DDP	coord: 2..59 e-value: 1.8E-19 score: 69.1
None	No IPR available	PANTHER	PTHR19338:SF15	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8-LIKE	coord: 2..59
IPR039238	Mitochondrial import inner membrane translocase subunit Tim8/13	PANTHER	PTHR19338	TRANSLOCASE OF INNER MITOCHONDRIAL MEMBRANE 13 HOMOLOG	coord: 2..59

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0029.1	MC06g0029.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0005758	mitochondrial intermembrane space
molecular_function	GO:0046872	metal ion binding