MC05g_new0271 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g_new0271
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionferredoxin-dependent glutamate synthase, chloroplastic
LocationMC05: 13605811 .. 13611196 (-)
RNA-Seq ExpressionMC05g_new0271
SyntenyMC05g_new0271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACTAACAAGGGCTTGGGCTTGTTGCTTGATAGCGACTTATTTGGGATGACGAAATCACAAGATAATGGATCCGAATGAGCAGAAAAACTAACGGTGATAAAGGCATCTGACACATGAGAAAAGGAATGGGAGTTTCCACAGCAGAAAGCAGCCAAGTCGTTGGAGTGTCTGCAGAAGCGAATCGCCAACTATAAACATCAACAACAACAACAGCAAAATCTGGACCAACGAAGCACAAACACTACAACTACAACTACAACTACAACGCACTACTAAGAGGTGAGGCGCGGGTGGCTCCCAACATTTCTCCACTCTCCTTATCCTTCTCTTCTCTTCTCACATCCATAATTTCCCACTTCCCGATCCTCCTCCCATGGCTCTGCAATCCGTTCCCTCCTCCATTTCCCACCTCCGCACCTCTACTCCCTCCCTCTCTGCTCATTCTCTCCTTCTATCGCCTAATAATGCTCTTTCGCTCGTCGATTTCGTTGCTCTGTATGGGAAGCCCAAGAGGACTAGGCGGAAATCTGTGTCCTATTCCTCCTATTCCTTGTCCGCCGCCGGCTGCCGGAGTTTCCGCCAGTTGGGTGCTAAGAACTCTTCGCCCTCGATCAAGGCTGTTCTTGATCTTCGACATGGTGGGTGTGCTGTGGATAAGTCTGATGCTCCTTCCGGACCCGAGCCCGAGGTATGCCAACTGGGTTCTGATTCTGAGTCTGATTCTGATTCTGATTCGGGTTTCGTTTTTGCAATGAATCACGTGAAGTAAGAGAAGTTTGAAGTGTCTTTACGGGAAGCTTTATCTTGTCTGTAAATCGTTATTACTTTTATTATTTTTTTTTTGCTTTGAAGCACATTTCATACCACACTTGAGGTTGAATTCTCTGTAGTTATCCTCCATCTTACATTCGATTTTAAATTTGGGAGTTCGAAATAATTGGTCATATTATATGATTCCCTGTACATAGCTAAGGAGTTCGAAATAATAAACATTTGCGAATCATATTTTATTTTACCATAAAGAATGGCGACGACACAATGGGGAAAATGATCAATGCATTTATGTTGTGTATGTGTTGGGTGGGGGGAGGTGGTCTTGGACTCTTGGGATATAAAGTAGCAACTATCATCCTATAGTTAGTTATACTTCTCCACTGTATGATGATTGATGCGTGTCTCCTTTCCCCATGAAAAAGAAGAGAAAAATTGGTCATTCCATTAATGGATATTTGAGCTGAGGAACGGCATTGAACATCTAACCCTTTTTCTCAATGTTGTTACTGGGAACCGTAGCCTGTTTAAGTATTTTGGAGTAACCAAATTTTAAAGTTTTTGTTCCATGAGGATTTCTGTGAAATAGTGATTGAGAATCCTACAATGGCCTCCATGCTTGACTAGAATGGTATTTGGATAAAGAGGCCCCTCAAGGAAAAAAGAGAATAGATAACACGTGTATCATATGTGATGGTATAAAATTTTGTTGTAGAGTTTGAGAATCATGTGGTCATTGCTAATGAAGTTTATATTTATCTCTCTTATTTTAATCAAAAAACTAGCTCTCATTGAGAAAATAAAAGAGCACAAAAGTGACAACCAAAACAACAACTCAATAGAGGAGGCATCCAAAAACTAAACTAGCTATACAAAAAAGGGCTTAAATCTAAAAGAACAAGGTCTAGAGGATAGTTATAAAATTCCTTGAAAACAGAAGGCAAAGGAAAGCATTTAATCTAACACAAGATTAGATCTCTTCCTAAAGGCCACTCAACACCATTAAAAATTCTCCTATTTCTCTCAAATCAAATCTCCCACAAAACGATAAAGAGTGCAATGCACTAGAGTACCTAATCCTTGTCACAAAATGGGGGATGGAAGAGCACTTCCTCCATCATAAAACAACTATCTCTTGTTTAGAGCCTAATCAATGCCAAAAGATTCCAAACCAGTCCCATACTGAGTTAGCAACCGACAACTTCATAGGACATGGTCAAAGTTGTCTAAATGCTCCTTGCAAAGAATGGACCACCGCAGCCCCAACAACGAGGAATGTCTAAGCATTTGTTCCACAATGTTCATCCTCCATGCATAGCTTGTCAAGCAAAGAACTCAACCTTATATTTATCTTCTCGAAAGTGGATTTCCAGCCAGTTTAACTCTCAACGTCCAGAAGTTTTGTGGGAGGTAGATTTCTTTTAGCAATTATTGTAATGGTAGGAAGATTTTGGTCGGTAGCCTACAGGAAGATTAATACTAATGAAAGGGTGTGAAAAAGGTGTCCCTGCAATGAGCAATGTATTTCTTCTGAGAAAGTGCAGGATTTTATTTTATTATTGTTTTTTGTGTGATAAGAAACAAACACTTCTCATTAAGGGGGATTTGGAGCGCGGGTTCGGTATCGGTAGCCTGAGAATGTTAAGTAAATTTGGGGGTGTTGTTGGGAAGCCTATATTTGGGGTGTGTAGCCAGAGTTTGGGTTTCTTTGAACTTTTTTTCAGAACTATTCCACCCTTTTACAATATTGTATTTCATTTTCTAAATTCAAAAAAAAAAAAGGTAAAAAAAAAGAAAAGAAAAGAAGATGGTAGGTTTACTATTCAACCTCATGGGATTATGTTTAGCAAGCCAAAGTTCCCAAACCCATGCTCAAAACATGAGTTTGGCTTACAATGTCAAACCAACCTCATTCCAACTCCTCCCCCAAAGCACCCCATAAAGGGAGTAAAAAGTAAAAGAGACCAAGGAGCTAAGTAAAGGCGCTCTAATTAATTAGAAAAGTGTGGAAAGTGTGACTATAATTCTTTGAGGTCTAAAAGTGTGAAGTGCAAAAGAGTTCTTTTTTGGCATCATTCATGGTTCGGTGATGTTTTGTTGAAGGACAGATTCCCCAATATTTATAGAATTTCCACGTCCCCTTCTATATTGGTAGAAGATGCGTGGGACAGAGGAGAGTCTAGTTCGCAAATTCTTACTAGGCGTTTGGTGAAAGATGAGGAGATTATTGAGTTAGCAAATCTTTTGTCCTTACTCACCTCGATTTTGCTTCATGAGACAGAGGATGTTTGGATTTGGAGTTTAGAGAATTCGGGCGTTTTCACCGTTAGCTCTTCAGGAAAGATTACGGCTGTTGTCTCTCTTTTTCCTAAGGATGTGTACTCCTCCCTTTGGAATTCGAATTGTCCAAAAAGGGTCAATATTTTGACTTGGATTCTTCTTATGGGCAAAGTAAATACATCAGAAATCTTGCAAAAAAAGTTGCCCTTTATGTTTCTACAGCCTACGGTTTGTCTATTGTGTGCTGCTAGTGGTGTGTTCCAGCAGAATGTCTTCTATTCTTGTTCTTAGGCAGTTGATTGTTGGGCTCTCTTCTTCTCTTTGTTCAACATCTCTTGGGTTTGGAATAGAGACCTCTAGTAATATCCTACAGCTCTATTGCGGTGCCCGTTTTCATAATAAAGCTAATCTCCTTTGGCTTAATGGGTTTAAAGCCATCATCTCAAAATTATGGTATGAAAGGAATTAGCAGGTTTTTGAAGGCAAACACAGATCTTGTATGGATTGTTTTTCTACAGCCAAGTATAAAGCTTCATAATGGTGTGCTCCCTCCGATCTTTTTTCTAATATTCTCAAAGTATGATTTGTATGAATTCGGAAACCTTTATAACTTCCCCTTAGAGATGTTATTGTTTGTTGTTATCATTGTTTCTGTTTTTGCTTCTTTTTGTTCTTATTTCACTCCTTTCGGAGTCTGTATCTTTGAACGTTTCTCATTTTCATCGATCAATGAAAAGTTTGTTTCTTGTTAAAAAAAGGGTGAGACTTTTTTTAAGCACAAAATAGAAAAGTATTAATATTTGTTATATAAGAACAATTAGTATTCTGAATAGTAAGATTAAAAAAAAGGTAAGAAATTAGAGCCTATCCAAGGGATTCTTTTGAGAAATGAGGTTCTTGGAACATATATTTAATTTATTTTTGATTTAAAAATAAAAAAATCAAACCAAGGGTGTGGCGCATAATGAATACTTTCATTTTTGCTTAAAAAAATAGAAAAAAGGCTAAAACAAGAGTGCTTTTTGCCAACTCATGCTTTCAAATCATGGGTAGTAGCGATAGACAAAAATCATTTAAACAATCTCATTAATAGCTATTATGTGGCAATTGTGAAGAGTAGCTAAAAGGAGAGCTTTTGAAGATAAAAGTAGTAATACTGTAACGATTTGGAATCTAATGCACTTTGTGGCTTCCTTTTTTGTTTTTTCTTATAAAATAATTTTGTAAATTTTGGTTGTTTTTGGCCTATGACTAGTTGGTGAACTTAGTCCTTATCTTTGATAGAGTTGGAGCAATGTTTCTTAAAGCGGAGGATTACAATTTACGTGGACATTAGAAAAATAAAAATATTATCAGATATTAAAAAAGCATAGCATCATGAATGATTAGATAATTAAAAAAATTAAAGAAATGAGGGAAGTTTTAAGAGATTGAGAATGCCAAGATGTGAACCTGGAGATTTAATTAAAGGCCAACATTTTGTCAACAAAGATAAAGTTATTTGGATTAATTTTATTTTGGCAATCATTTGGAAATTTGGAATGAGTGAAATCTCAAAATCTGAAACATAGGAAACAGAGACTTGAGAAATCCAGGACTTGGGAAGATATTTAATCCTTCACTCATATTTCTGTATTCTTTTTGCACGTTAATGCAATTTTCTTAAAAAATTGGGACTTGTTTGAGCAGCTTATTCAGCATGCCATACTTTCTTAATTCATTGCGTGTTATGCCTGTAATCGAAGCATTTTGTTCCAACTGTATATATGATGTAATTTGATGCTGATCAGGTTGCAAATTTGGATGACATAATATCAGAAAGAGGAGCATGTGGAGTTGGATTTGTTGCCAACTTGGAAAACAAAGCATCCCACAAGATTGTCCAGGATGCTCTTACAGCACTAGGCTGTATGGAACATCGTGGTGGATGTGGAGCTGATAATGATTCTGGTGATGGTTCAGGATTGATGACTTCCATCCCATGGGATCTTTTTGATGATTGGGCCAACAGGCAAGGGATTCCTTCGTTTGATAAGTTGCATGCAGGCGTTGGCATGGTTTTTCTACCCAAAGATGATGGTCATAGTAACGAGGCAAAAGAAGGTGATTGTTCAATTATAATTGATCAAGTTGAAACAAATTAATTGTTTCTTTCTATTTATATAGGGGACAACTAAGGATTTTTGTGATTACTCTTTTATCTTTGACTTTTTTGTGATGGTGTAGTTTGTTAGTTTTTTCATTTTTTATCTTGGCTCCTTTTGTTTTTTTTTTACTAATCGTCCTTTATGTATTTAATTTCTTCTCAATGAAAGTTAGCTTCTTCATTAATA

mRNA sequence

ATGGCTCTGCAATCCGTTCCCTCCTCCATTTCCCACCTCCGCACCTCTACTCCCTCCCTCTCTGCTCATTCTCTCCTTCTATCGCCTAATAATGCTCTTTCGCTCGTCGATTTCGTTGCTCTGTATGGGAAGCCCAAGAGGACTAGGCGGAAATCTGTGTCCTATTCCTCCTATTCCTTGTCCGCCGCCGGCTGCCGGAGTTTCCGCCAGTTGGGTGCTAAGAACTCTTCGCCCTCGATCAAGGCTGTTCTTGATCTTCGACATGGTGGGTGTGCTGTGGATAAGTCTGATGCTCCTTCCGGACCCGAGCCCGAGGTTGCAAATTTGGATGACATAATATCAGAAAGAGGAGCATGTGGAGTTGGATTTGTTGCCAACTTGGAAAACAAAGCATCCCACAAGATTGTCCAGGATGCTCTTACAGCACTAGGCTGTATGGAACATCGTGGTGGATGTGGAGCTGATAATGATTCTGGTGATGGTTCAGGATTGATGACTTCCATCCCATGGGATCTTTTTGATGATTGGGCCAACAGGCAAGGGATTCCTTCGTTTGATAAGTTGCATGCAGGCGTTGGCATGGTTTTTCTACCCAAAGATGATGGTCATAGTAACGAGGCAAAAGAAGGTGATTGTTCAATTATAATTGATCAAGTTGAAACAAATTAA

Coding sequence (CDS)

ATGGCTCTGCAATCCGTTCCCTCCTCCATTTCCCACCTCCGCACCTCTACTCCCTCCCTCTCTGCTCATTCTCTCCTTCTATCGCCTAATAATGCTCTTTCGCTCGTCGATTTCGTTGCTCTGTATGGGAAGCCCAAGAGGACTAGGCGGAAATCTGTGTCCTATTCCTCCTATTCCTTGTCCGCCGCCGGCTGCCGGAGTTTCCGCCAGTTGGGTGCTAAGAACTCTTCGCCCTCGATCAAGGCTGTTCTTGATCTTCGACATGGTGGGTGTGCTGTGGATAAGTCTGATGCTCCTTCCGGACCCGAGCCCGAGGTTGCAAATTTGGATGACATAATATCAGAAAGAGGAGCATGTGGAGTTGGATTTGTTGCCAACTTGGAAAACAAAGCATCCCACAAGATTGTCCAGGATGCTCTTACAGCACTAGGCTGTATGGAACATCGTGGTGGATGTGGAGCTGATAATGATTCTGGTGATGGTTCAGGATTGATGACTTCCATCCCATGGGATCTTTTTGATGATTGGGCCAACAGGCAAGGGATTCCTTCGTTTGATAAGTTGCATGCAGGCGTTGGCATGGTTTTTCTACCCAAAGATGATGGTCATAGTAACGAGGCAAAAGAAGGTGATTGTTCAATTATAATTGATCAAGTTGAAACAAATTAA

Protein sequence

MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSLSAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSIIIDQVETN
Homology
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BLAST of MC05g_new0271 vs. ExPASy Swiss-Prot
Match: Q9ZNZ7 (Ferredoxin-dependent glutamate synthase 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLU1 PE=1 SV=3)

HSP 1 Score: 204.1 bits (518), Expect = 1.6e-51
Identity = 114/211 (54.03%), Postives = 141/211 (66.82%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60
           MA+QS+ S +  L ++TP     S +LS +     VDFV LY K KRTRR+        L
Sbjct: 1   MAMQSL-SPVPKLLSTTP-----SSVLSSDKNFFFVDFVGLYCKSKRTRRR--------L 60

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLR--HGGCAVDKSDAPSGPEPEVANLDDIISERGA 120
                 S R   + +   S++AV+DL   HG    D S +PS   P+VANL+DI+SERGA
Sbjct: 61  RGDSSSSSRSSSSLSRLSSVRAVIDLERVHGVSEKDLS-SPSALRPQVANLEDILSERGA 120

Query: 121 CGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWAN 180
           CGVGF+ANL+N  SH +V+DAL ALGCMEHRGGCGADNDSGDGSGLM+SIPWD F+ WA 
Sbjct: 121 CGVGFIANLDNIPSHGVVKDALIALGCMEHRGGCGADNDSGDGSGLMSSIPWDFFNVWAK 180

Query: 181 RQGIPSFDKLHAGVGMVFLPKDDGHSNEAKE 210
            Q +  FDKLH GVGM+FLP+DD    EAK+
Sbjct: 181 EQSLAPFDKLHTGVGMIFLPQDDTFMQEAKQ 196

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BLAST of MC05g_new0271 vs. ExPASy Swiss-Prot
Match: Q9T0P4 (Ferredoxin-dependent glutamate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLU2 PE=1 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 9.7e-49
Identity = 116/221 (52.49%), Postives = 142/221 (64.25%), Query Frame = 0 

Query: 1   MALQS-----VPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSY 60
           MALQS       SS+S L +S          LS    +  VDFV  Y   K T+R++   
Sbjct: 1   MALQSPGATGASSSVSRLLSSAK--------LSSTKTIFSVDFVRSYCISKGTKRRN--- 60

Query: 61  SSYSLSAAGCRSFRQL--GAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDII 120
                  +G R +  L   +  S  S+KA+L+      A D S + S  +P+VA L+DII
Sbjct: 61  -----ELSGFRGYSPLLKSSLRSPFSVKAILN--SDRAAGDASSSFSDLKPQVAYLEDII 120

Query: 121 SERGACGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLF 180
           SERGACGVGF+ANLENKA+HKIV DAL ALGCMEHRGGCG+DN SGDGSGLMTSIPWDLF
Sbjct: 121 SERGACGVGFIANLENKATHKIVNDALIALGCMEHRGGCGSDNTSGDGSGLMTSIPWDLF 180

Query: 181 DDWANRQGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSI 215
           ++WA +QGI SFD+ H GVGM+FLP+DD    EAK+   SI
Sbjct: 181 NEWAEKQGIASFDRTHTGVGMLFLPRDDNIRKEAKKVITSI 203

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BLAST of MC05g_new0271 vs. ExPASy Swiss-Prot
Match: P23225 (Ferredoxin-dependent glutamate synthase, chloroplastic OS=Zea mays OX=4577 GN=GLSF PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.0e-41
Identity = 88/159 (55.35%), Postives = 116/159 (72.96%), Query Frame = 0 

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCA-VDKSDAPSGPEPEVANLDDIISERGAC 120
           SAA  RS+  + A  +S S +AV+    GG A  +   AP  P  + A+L+ I+SERGAC
Sbjct: 45  SAAARRSW--VVASAASSSSRAVV----GGVARREAPPAPQKPTQQAADLNHILSERGAC 104

Query: 121 GVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANR 180
           GVGFVANL+N +S  IV+DAL ALGCMEHRGGCGAD+DSGDG+GLM+++PWDLFDDWA++
Sbjct: 105 GVGFVANLKNMSSFDIVRDALMALGCMEHRGGCGADSDSGDGAGLMSAVPWDLFDDWASK 164

Query: 181 QGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSIIIDQ 219
           QG+  FD+ + GVGMVFLP+D+    EAK     + +D+
Sbjct: 165 QGLALFDRRNTGVGMVFLPQDEKSMEEAKAATEKVFVDE 197

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BLAST of MC05g_new0271 vs. ExPASy Swiss-Prot
Match: Q69RJ0 (Ferredoxin-dependent glutamate synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=GLU PE=2 SV=2)

HSP 1 Score: 170.6 bits (431), Expect = 2.0e-41
Identity = 89/176 (50.57%), Postives = 115/176 (65.34%), Query Frame = 0 

Query: 45  PKRTRRKSVSYSSYSLSAAGCRSFRQLGAKN--SSPSIKAVLDLRHGGCAVDKSDAPSGP 104
           P    R +   ++  L A G R+     A+      S +AVLDL        + +A   P
Sbjct: 27  PLLAGRAAARSAARRLRARGTRAPPLAAARRGWGGVSPRAVLDLPR------RREAAEKP 86

Query: 105 EPEVANLDDIISERGACGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGS 164
             + A+L++I+SERGACGVGFVANL+N+ S  IV+DAL ALGCMEHRGGCGADNDSGDGS
Sbjct: 87  AQKAADLNEILSERGACGVGFVANLKNEPSFNIVRDALVALGCMEHRGGCGADNDSGDGS 146

Query: 165 GLMTSIPWDLFDDWANRQGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSIIIDQ 219
           GLM+ IPWDLF+DWAN+QG+   D+ + GVGMVFLP+D+    EAK     +  D+
Sbjct: 147 GLMSGIPWDLFNDWANKQGLAPLDRTNTGVGMVFLPQDENSMEEAKAVVAKVFTDE 196

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BLAST of MC05g_new0271 vs. ExPASy Swiss-Prot
Match: Q43155 (Ferredoxin-dependent glutamate synthase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FdGOGAT PE=1 SV=3)

HSP 1 Score: 153.7 bits (387), Expect = 2.5e-36
Identity = 68/91 (74.73%), Postives = 79/91 (86.81%), Query Frame = 0 

Query: 119 CGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWAN 178
           CGVGF+ANL+NK S +IV+DALTALGCMEHRGGCG+DNDSGDGSG+MT+IPWDLF+DW  
Sbjct: 1   CGVGFIANLDNKGSFQIVKDALTALGCMEHRGGCGSDNDSGDGSGVMTAIPWDLFNDWGK 60

Query: 179 RQGIPSFDKLHAGVGMVFLPKDDGHSNEAKE 210
            QGI  FD+ H GVGMVFLPKDD  + EAK+
Sbjct: 61  DQGIGPFDRSHTGVGMVFLPKDDLLAEEAKK 91

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BLAST of MC05g_new0271 vs. NCBI nr
Match: XP_022147781.1 (ferredoxin-dependent glutamate synthase, chloroplastic [Momordica charantia])

HSP 1 Score: 419 bits (1078), Expect = 7.14e-133
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60
           MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL
Sbjct: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG 120
           SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG
Sbjct: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG 120

Query: 121 VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ 180
           VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ
Sbjct: 121 VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ 180

Query: 181 GIPSFDKLHAGVGMVFLPKDDGHSNEAKE 209
           GIPSFDKLHAGVGMVFLPKDDGHSNEAKE
Sbjct: 181 GIPSFDKLHAGVGMVFLPKDDGHSNEAKE 209

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BLAST of MC05g_new0271 vs. NCBI nr
Match: KAG7017639.1 (Ferredoxin-dependent glutamate synthase 1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303 bits (775), Expect = 2.56e-98
Identity = 163/215 (75.81%), Postives = 176/215 (81.86%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKS-VSYSSYS 60
           MAL S  SSISHLR S+PSLS HS LLSP+  LSL+DFVA YG+  RTRRKS +SYSS S
Sbjct: 1   MALPSASSSISHLRPSSPSLSPHSPLLSPHG-LSLLDFVAFYGRSNRTRRKSFLSYSSSS 60

Query: 61  LSAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGAC 120
           LS    RS R   ++  S SIKAVLDL   G A+D SDAPS P P+VANL+DIISERGAC
Sbjct: 61  LSN---RSIRHFSSRKFSSSIKAVLDLPLPGSALDNSDAPSEPVPQVANLNDIISERGAC 120

Query: 121 GVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANR 180
           GVGFVANLENKASHKI+QDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFD+WAN 
Sbjct: 121 GVGFVANLENKASHKIIQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDNWANE 180

Query: 181 QGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSI 214
           QGIPSFDKLHAG+GMVFLP DDG   EAKE   SI
Sbjct: 181 QGIPSFDKLHAGIGMVFLPNDDGQYKEAKEVIASI 211

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BLAST of MC05g_new0271 vs. NCBI nr
Match: KAG6596173.1 (Ferredoxin-dependent glutamate synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 286 bits (731), Expect = 2.39e-94
Identity = 151/209 (72.25%), Postives = 165/209 (78.95%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60
           MAL SV SSIS L   +P       LLS N+ LSL+DF+A +G  KR  RKS+SYS +S 
Sbjct: 1   MALPSVSSSISRLTPPSP-------LLSHNHTLSLLDFIATHGTSKRIARKSLSYS-HSS 60

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG 120
           S    R+FR LG+ +SS SIKA L L     AVD SDAP   +P+VANLDDIISERGACG
Sbjct: 61  SLLPIRTFRHLGSSHSSSSIKARLHLPRAASAVDSSDAPPQHQPKVANLDDIISERGACG 120

Query: 121 VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ 180
           VGFVANLENKASHKI+QDALTAL CMEHRGGCGADNDSGDGSGLMTSIPWDLFD+WAN Q
Sbjct: 121 VGFVANLENKASHKIIQDALTALSCMEHRGGCGADNDSGDGSGLMTSIPWDLFDNWANEQ 180

Query: 181 GIPSFDKLHAGVGMVFLPKDDGHSNEAKE 209
           GIPSFDKLHAGVGMVFLPKDDGH  EAKE
Sbjct: 181 GIPSFDKLHAGVGMVFLPKDDGHIKEAKE 201

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BLAST of MC05g_new0271 vs. NCBI nr
Match: XP_022934447.1 (ferredoxin-dependent glutamate synthase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 310 bits (794), Expect = 1.98e-93
Identity = 164/219 (74.89%), Postives = 178/219 (81.28%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKS-VSYSSYS 60
           MAL S  SSISHLR S+PSLS HS LLSPN+ LSL+DFVA YG+  RTRRKS +SYSS S
Sbjct: 1   MALPSASSSISHLRPSSPSLSPHSPLLSPNHGLSLLDFVAFYGRSNRTRRKSFLSYSSSS 60

Query: 61  LSAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGAC 120
           LS    RS R   ++  S SIKAVLDL   G A+D SDAPS P P+VANL+DIISERGAC
Sbjct: 61  LSN---RSIRHFSSRKFSSSIKAVLDLPLPGSALDNSDAPSEPVPQVANLNDIISERGAC 120

Query: 121 GVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANR 180
           GVGFVANLENKASHKI+QDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFD+WAN 
Sbjct: 121 GVGFVANLENKASHKIIQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDNWANE 180

Query: 181 QGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSIIIDQ 218
           QGIPSFDKLHAG+GMVFLP DDG   EAKE   SI   +
Sbjct: 181 QGIPSFDKLHAGIGMVFLPNDDGQYKEAKEVIASIFTQE 216

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BLAST of MC05g_new0271 vs. NCBI nr
Match: XP_023526491.1 (ferredoxin-dependent glutamate synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 306 bits (783), Expect = 6.12e-92
Identity = 162/215 (75.35%), Postives = 176/215 (81.86%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKS-VSYSSYS 60
           MAL S  SSISHLR S+PSLS HS LLSPN+ LSL+DFVA YG+  RTRRKS +SYSS S
Sbjct: 1   MALPSASSSISHLRPSSPSLSPHSPLLSPNHGLSLLDFVAFYGRSNRTRRKSFLSYSSSS 60

Query: 61  LSAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGAC 120
           LS    R+     ++  S SIKAVLDL   G A+D SDAPS P P+VANL+DIISERGAC
Sbjct: 61  LSN---RNIPHFSSRKFSSSIKAVLDLPLPGSALDNSDAPSEPVPQVANLNDIISERGAC 120

Query: 121 GVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANR 180
           GVGFVANLENKASHKI+QDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFD+WAN 
Sbjct: 121 GVGFVANLENKASHKIIQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDNWANE 180

Query: 181 QGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSI 214
           QGIPSFDKLHAG+GMVFLP DDG   EAKE   SI
Sbjct: 181 QGIPSFDKLHAGIGMVFLPNDDGQYKEAKEVIASI 212

[Close]
BLAST of MC05g_new0271 vs. ExPASy TrEMBL
Match: A0A6J1D3E3 (ferredoxin-dependent glutamate synthase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016634 PE=3 SV=1)

HSP 1 Score: 419 bits (1078), Expect = 3.45e-133
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60
           MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL
Sbjct: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG 120
           SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG
Sbjct: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG 120

Query: 121 VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ 180
           VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ
Sbjct: 121 VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ 180

Query: 181 GIPSFDKLHAGVGMVFLPKDDGHSNEAKE 209
           GIPSFDKLHAGVGMVFLPKDDGHSNEAKE
Sbjct: 181 GIPSFDKLHAGVGMVFLPKDDGHSNEAKE 209

[Close]
BLAST of MC05g_new0271 vs. ExPASy TrEMBL
Match: A0A6J1F2S6 (ferredoxin-dependent glutamate synthase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441624 PE=3 SV=1)

HSP 1 Score: 310 bits (794), Expect = 9.56e-94
Identity = 164/219 (74.89%), Postives = 178/219 (81.28%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKS-VSYSSYS 60
           MAL S  SSISHLR S+PSLS HS LLSPN+ LSL+DFVA YG+  RTRRKS +SYSS S
Sbjct: 1   MALPSASSSISHLRPSSPSLSPHSPLLSPNHGLSLLDFVAFYGRSNRTRRKSFLSYSSSS 60

Query: 61  LSAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGAC 120
           LS    RS R   ++  S SIKAVLDL   G A+D SDAPS P P+VANL+DIISERGAC
Sbjct: 61  LSN---RSIRHFSSRKFSSSIKAVLDLPLPGSALDNSDAPSEPVPQVANLNDIISERGAC 120

Query: 121 GVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANR 180
           GVGFVANLENKASHKI+QDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFD+WAN 
Sbjct: 121 GVGFVANLENKASHKIIQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDNWANE 180

Query: 181 QGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSIIIDQ 218
           QGIPSFDKLHAG+GMVFLP DDG   EAKE   SI   +
Sbjct: 181 QGIPSFDKLHAGIGMVFLPNDDGQYKEAKEVIASIFTQE 216

[Close]
BLAST of MC05g_new0271 vs. ExPASy TrEMBL
Match: A0A6J1J320 (ferredoxin-dependent glutamate synthase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482225 PE=3 SV=1)

HSP 1 Score: 302 bits (773), Expect = 6.72e-91
Identity = 158/214 (73.83%), Postives = 173/214 (80.84%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60
           MAL S  SSISHLR S+P    HS LLSPN+ LSL+DFVA YG+  RTRRKS  + SYS 
Sbjct: 1   MALLSASSSISHLRPSSP----HSPLLSPNHGLSLLDFVAFYGRSNRTRRKS--FLSYSF 60

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACG 120
           S+   R+ RQ  ++  S SIKAVLDL   G A+D SDAPS P P+VANL+DIISERGACG
Sbjct: 61  SSLSNRNIRQFSSRKFSSSIKAVLDLPLPGSALDNSDAPSEPVPQVANLNDIISERGACG 120

Query: 121 VGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQ 180
           VGFVANLENKASHKI+QDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFD+WAN Q
Sbjct: 121 VGFVANLENKASHKIIQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDNWANEQ 180

Query: 181 GIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSI 214
           GIPSFDKLHAG+GMVFLP DDG   EAKE   SI
Sbjct: 181 GIPSFDKLHAGIGMVFLPNDDGQYKEAKEVIASI 208

[Close]
BLAST of MC05g_new0271 vs. ExPASy TrEMBL
Match: A0A0A0LCE0 (Glutamine amidotransferase type-2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G821030 PE=3 SV=1)

HSP 1 Score: 290 bits (741), Expect = 1.44e-86
Identity = 159/216 (73.61%), Postives = 174/216 (80.56%), Query Frame = 0 

Query: 1   MALQSVPSSISHLR-TSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRK-SVSYSSY 60
           MA  S+ SSISHL  +S+PSLS HS LLSPN +LSL+DFVA YG+  RTRRK S+SYSS 
Sbjct: 1   MAFPSISSSISHLPPSSSPSLSPHSPLLSPNPSLSLLDFVAFYGRSNRTRRKPSLSYSSS 60

Query: 61  SLSAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGA 120
           SLS    RSFR   + NSS SIKAVLDL         S + S P P+VANL+DIISERGA
Sbjct: 61  SLSTR--RSFRHFTSSNSSSSIKAVLDLP---LRPSSSSSSSEPVPKVANLEDIISERGA 120

Query: 121 CGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWAN 180
           CGVGFVANLENKASHKI+QDALTALGCMEHRGGCGADNDSGDGSGLM+SIPWDLFD+WAN
Sbjct: 121 CGVGFVANLENKASHKIIQDALTALGCMEHRGGCGADNDSGDGSGLMSSIPWDLFDNWAN 180

Query: 181 RQGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSI 214
            QGIPSFDKLH GVGMVFLPKDDG + EAKE   SI
Sbjct: 181 GQGIPSFDKLHTGVGMVFLPKDDGDNKEAKEVVASI 211

[Close]
BLAST of MC05g_new0271 vs. ExPASy TrEMBL
Match: A0A1S3B8C0 (ferredoxin-dependent glutamate synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486888 PE=3 SV=1)

HSP 1 Score: 286 bits (731), Expect = 3.21e-85
Identity = 158/216 (73.15%), Postives = 172/216 (79.63%), Query Frame = 0 

Query: 1   MALQSVPSSISHLR-TSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKS-VSYSSY 60
           MA  S+ SSISHL  +S+PSLS HS LLSPN +LSL+DFVA YG+  RTRRKS +SYSS 
Sbjct: 1   MAFPSISSSISHLPPSSSPSLSPHSPLLSPNPSLSLLDFVAFYGRSNRTRRKSSLSYSSS 60

Query: 61  SLSAAGCRSFRQLGAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGA 120
           S S    RSFR   + NSS SIKAVLDL         S + S P P+VANLDDIISERGA
Sbjct: 61  SFSTR--RSFRHFTSSNSSSSIKAVLDLP----LRTSSSSSSEPLPQVANLDDIISERGA 120

Query: 121 CGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWAN 180
           CGVGFVANLENKASHKI+QDALTALGCMEHRGGCGADNDSGDGSGLM+SIPWDLFD+WA+
Sbjct: 121 CGVGFVANLENKASHKIIQDALTALGCMEHRGGCGADNDSGDGSGLMSSIPWDLFDNWAS 180

Query: 181 RQGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSI 214
            QGIPSFDKLH GVGMVFLPKDDG   EAKE   SI
Sbjct: 181 GQGIPSFDKLHTGVGMVFLPKDDGDYKEAKEVVASI 210

[Close]
BLAST of MC05g_new0271 vs. TAIR 10
Match: AT5G04140.1 (glutamate synthase 1 )

HSP 1 Score: 204.1 bits (518), Expect = 1.1e-52
Identity = 114/211 (54.03%), Postives = 141/211 (66.82%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60
           MA+QS+ S +  L ++TP     S +LS +     VDFV LY K KRTRR+        L
Sbjct: 1   MAMQSL-SPVPKLLSTTP-----SSVLSSDKNFFFVDFVGLYCKSKRTRRR--------L 60

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLR--HGGCAVDKSDAPSGPEPEVANLDDIISERGA 120
                 S R   + +   S++AV+DL   HG    D S +PS   P+VANL+DI+SERGA
Sbjct: 61  RGDSSSSSRSSSSLSRLSSVRAVIDLERVHGVSEKDLS-SPSALRPQVANLEDILSERGA 120

Query: 121 CGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWAN 180
           CGVGF+ANL+N  SH +V+DAL ALGCMEHRGGCGADNDSGDGSGLM+SIPWD F+ WA 
Sbjct: 121 CGVGFIANLDNIPSHGVVKDALIALGCMEHRGGCGADNDSGDGSGLMSSIPWDFFNVWAK 180

Query: 181 RQGIPSFDKLHAGVGMVFLPKDDGHSNEAKE 210
            Q +  FDKLH GVGM+FLP+DD    EAK+
Sbjct: 181 EQSLAPFDKLHTGVGMIFLPQDDTFMQEAKQ 196

[Close]
BLAST of MC05g_new0271 vs. TAIR 10
Match: AT2G41220.1 (glutamate synthase 2 )

HSP 1 Score: 194.9 bits (494), Expect = 6.9e-50
Identity = 116/221 (52.49%), Postives = 142/221 (64.25%), Query Frame = 0 

Query: 1   MALQS-----VPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSY 60
           MALQS       SS+S L +S          LS    +  VDFV  Y   K T+R++   
Sbjct: 1   MALQSPGATGASSSVSRLLSSAK--------LSSTKTIFSVDFVRSYCISKGTKRRN--- 60

Query: 61  SSYSLSAAGCRSFRQL--GAKNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDII 120
                  +G R +  L   +  S  S+KA+L+      A D S + S  +P+VA L+DII
Sbjct: 61  -----ELSGFRGYSPLLKSSLRSPFSVKAILN--SDRAAGDASSSFSDLKPQVAYLEDII 120

Query: 121 SERGACGVGFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLF 180
           SERGACGVGF+ANLENKA+HKIV DAL ALGCMEHRGGCG+DN SGDGSGLMTSIPWDLF
Sbjct: 121 SERGACGVGFIANLENKATHKIVNDALIALGCMEHRGGCGSDNTSGDGSGLMTSIPWDLF 180

Query: 181 DDWANRQGIPSFDKLHAGVGMVFLPKDDGHSNEAKEGDCSI 215
           ++WA +QGI SFD+ H GVGM+FLP+DD    EAK+   SI
Sbjct: 181 NEWAEKQGIASFDRTHTGVGMLFLPRDDNIRKEAKKVITSI 203

[Close]
BLAST of MC05g_new0271 vs. TAIR 10
Match: AT5G04140.2 (glutamate synthase 1 )

HSP 1 Score: 189.9 bits (481), Expect = 2.2e-48
Identity = 114/237 (48.10%), Postives = 141/237 (59.49%), Query Frame = 0 

Query: 1   MALQSVPSSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSL 60
           MA+QS+ S +  L ++TP     S +LS +     VDFV LY K KRTRR+        L
Sbjct: 1   MAMQSL-SPVPKLLSTTP-----SSVLSSDKNFFFVDFVGLYCKSKRTRRR--------L 60

Query: 61  SAAGCRSFRQLGAKNSSPSIKAVLDLR--HGGCAVDKSDAPSGPEP-------------- 120
                 S R   + +   S++AV+DL   HG    D S +PS   P              
Sbjct: 61  RGDSSSSSRSSSSLSRLSSVRAVIDLERVHGVSEKDLS-SPSALRPQVRFFTDINFTNTQ 120

Query: 121 ------------EVANLDDIISERGACGVGFVANLENKASHKIVQDALTALGCMEHRGGC 180
                       +VANL+DI+SERGACGVGF+ANL+N  SH +V+DAL ALGCMEHRGGC
Sbjct: 121 RAKFHPLWGSFKQVANLEDILSERGACGVGFIANLDNIPSHGVVKDALIALGCMEHRGGC 180

Query: 181 GADNDSGDGSGLMTSIPWDLFDDWANRQGIPSFDKLHAGVGMVFLPKDDGHSNEAKE 210
           GADNDSGDGSGLM+SIPWD F+ WA  Q +  FDKLH GVGM+FLP+DD    EAK+
Sbjct: 181 GADNDSGDGSGLMSSIPWDFFNVWAKEQSLAPFDKLHTGVGMIFLPQDDTFMQEAKQ 222

[Close]
BLAST of MC05g_new0271 vs. TAIR 10
Match: AT5G53460.1 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 77.8 bits (190), Expect = 1.2e-14
Identity = 65/207 (31.40%), Postives = 95/207 (45.89%), Query Frame = 0 

Query: 8   SSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSLSAAGCRS 67
           SS+ HLRT+   LS  SL  S + A  L     +  +   T R SV       S      
Sbjct: 7   SSVLHLRTNQQLLSLRSLKNSTSVASQLAVTSGVSRRRSCTARCSVKKPVIPESPFLGTR 66

Query: 68  FRQLGA------KNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACGV 127
            R+ G+      ++  P   A L        V KS   + PE  +  L D   ++ +CGV
Sbjct: 67  VRRSGSETLQFWRSDGPGRSAKLR------TVVKSSFSAVPEKPL-GLYDPSYDKDSCGV 126

Query: 128 GFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQG 187
           GFVA L  + + K V D+L  L  M HRG CG ++++GDG+G++  +P D + + A   G
Sbjct: 127 GFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELG 186

Query: 188 IPSFDKLHAGVGMVFLPKDDGHSNEAK 209
                  +  VGM FLP  +    E+K
Sbjct: 187 FVLPSAGNYAVGMFFLPTVESRREESK 206

[Close]
BLAST of MC05g_new0271 vs. TAIR 10
Match: AT5G53460.2 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 77.8 bits (190), Expect = 1.2e-14
Identity = 65/207 (31.40%), Postives = 95/207 (45.89%), Query Frame = 0 

Query: 8   SSISHLRTSTPSLSAHSLLLSPNNALSLVDFVALYGKPKRTRRKSVSYSSYSLSAAGCRS 67
           SS+ HLRT+   LS  SL  S + A  L     +  +   T R SV       S      
Sbjct: 7   SSVLHLRTNQQLLSLRSLKNSTSVASQLAVTSGVSRRRSCTARCSVKKPVIPESPFLGTR 66

Query: 68  FRQLGA------KNSSPSIKAVLDLRHGGCAVDKSDAPSGPEPEVANLDDIISERGACGV 127
            R+ G+      ++  P   A L        V KS   + PE  +  L D   ++ +CGV
Sbjct: 67  VRRSGSETLQFWRSDGPGRSAKLR------TVVKSSFSAVPEKPL-GLYDPSYDKDSCGV 126

Query: 128 GFVANLENKASHKIVQDALTALGCMEHRGGCGADNDSGDGSGLMTSIPWDLFDDWANRQG 187
           GFVA L  + + K V D+L  L  M HRG CG ++++GDG+G++  +P D + + A   G
Sbjct: 127 GFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELG 186

Query: 188 IPSFDKLHAGVGMVFLPKDDGHSNEAK 209
                  +  VGM FLP  +    E+K
Sbjct: 187 FVLPSAGNYAVGMFFLPTVESRREESK 206

The following BLAST results are available for this feature:
BLAST of MC05g_new0271 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZNZ71.6e-5154.03Ferredoxin-dependent glutamate synthase 1, chloroplastic/mitochondrial OS=Arabid... [more]
Q9T0P49.7e-4952.49Ferredoxin-dependent glutamate synthase 2, chloroplastic OS=Arabidopsis thaliana... [more]
P232252.0e-4155.35Ferredoxin-dependent glutamate synthase, chloroplastic OS=Zea mays OX=4577 GN=GL... [more]
Q69RJ02.0e-4150.57Ferredoxin-dependent glutamate synthase, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q431552.5e-3674.73Ferredoxin-dependent glutamate synthase, chloroplastic OS=Spinacia oleracea OX=3... [more]
BLAST of MC05g_new0271 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022147781.17.14e-133100.00ferredoxin-dependent glutamate synthase, chloroplastic [Momordica charantia][more]
KAG7017639.12.56e-9875.81Ferredoxin-dependent glutamate synthase 1, chloroplastic/mitochondrial, partial ... [more]
KAG6596173.12.39e-9472.25Ferredoxin-dependent glutamate synthase 2, chloroplastic, partial [Cucurbita arg... [more]
XP_022934447.11.98e-9374.89ferredoxin-dependent glutamate synthase, chloroplastic-like isoform X1 [Cucurbit... [more]
XP_023526491.16.12e-9275.35ferredoxin-dependent glutamate synthase, chloroplastic-like [Cucurbita pepo subs... [more]
BLAST of MC05g_new0271 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D3E33.45e-133100.00ferredoxin-dependent glutamate synthase, chloroplastic OS=Momordica charantia OX... [more]
A0A6J1F2S69.56e-9474.89ferredoxin-dependent glutamate synthase, chloroplastic-like isoform X1 OS=Cucurb... [more]
A0A6J1J3206.72e-9173.83ferredoxin-dependent glutamate synthase, chloroplastic-like isoform X1 OS=Cucurb... [more]
A0A0A0LCE01.44e-8673.61Glutamine amidotransferase type-2 domain-containing protein OS=Cucumis sativus O... [more]
A0A1S3B8C03.21e-8573.15ferredoxin-dependent glutamate synthase, chloroplastic isoform X1 OS=Cucumis mel... [more]
BLAST of MC05g_new0271 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G04140.11.1e-5254.03glutamate synthase 1 [more]
AT2G41220.16.9e-5052.49glutamate synthase 2 [more]
AT5G04140.22.2e-4848.10glutamate synthase 1 [more]
AT5G53460.11.2e-1431.40NADH-dependent glutamate synthase 1 [more]
AT5G53460.21.2e-1431.40NADH-dependent glutamate synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017932Glutamine amidotransferase type 2 domainPFAMPF00310GATase_2coord: 119..209
e-value: 6.5E-30
score: 104.2
IPR017932Glutamine amidotransferase type 2 domainPROSITEPS51278GATASE_TYPE_2coord: 119..222
score: 10.201118
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 119..218
e-value: 2.3E-35
score: 124.1
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 119..201
NoneNo IPR availablePANTHERPTHR11938FAD NADPH DEHYDROGENASE/OXIDOREDUCTASEcoord: 76..214
NoneNo IPR availablePANTHERPTHR11938:SF134FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE 2, CHLOROPLASTICcoord: 76..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g_new0271.1MC05g_new0271.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019676 ammonia assimilation cycle
biological_process GO:0006537 glutamate biosynthetic process
molecular_function GO:0051538 3 iron, 4 sulfur cluster binding
molecular_function GO:0016040 glutamate synthase (NADH) activity
molecular_function GO:0046872 metal ion binding