MC05g_new0080 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g_new0080 vs. ExPASy Swiss-Prot
Match: F4JAU2 (SAC3 family protein B OS=Arabidopsis thaliana OX=3702 GN=SAC3B PE=1 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 4.3e-308
Identity = 718/1684 (42.64%), Postives = 968/1684 (57.48%), Query Frame = 0

Query: 7    GKASGPS---APP-----KLQHSFGSSARPVSVS--------PSPSTF--PAFSDASPPR 66
            G  SGPS   APP       Q S G   RP  V         PSPS F  P+ S   P +
Sbjct: 72   GIRSGPSIQRAPPLSASQNPQLSIGKPYRPGGVQSVPPINRIPSPSAFQNPSPSSGQPYQ 131

Query: 67   APRVQSHPMAFQTTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSR 126
               +Q  P  F   G A         GP A            S  S +   Y +P  G +
Sbjct: 132  PGGIQRIPEPF--NGIA--------WGPEA------------SRTSPSVRPYQFP--GVQ 191

Query: 127  GPILFPTRSVE---------EQHSSFKAVDAQSH--PSRGITSVASPQTSDHAVGEVAHL 186
             P L P    +          +HS   +  A SH     G  +V   ++ D      + +
Sbjct: 192  RPNLNPQYGHDGSRNFLKDHGEHSRATSPPATSHILSRMGTDAVEIGRSQDSKRKSRSDI 251

Query: 187  L-------TKRSRSPTYMPGDEKLSKIAGHPVI--RRPASSSHTFDNQPKMRGIHVNSHS 246
            L       ++R++SP  + G E  + + G   +  R    S  + +N P     + N   
Sbjct: 252  LPDQNMGFSRRNQSP--VSGFENGNLVDGFQPLSSRTWMRSPSSAENNPVRSRSNPNQLI 311

Query: 247  YQDQ-----YPSKRSYDQDTNILTGFGNVQAPKGNRSPEQPSASDLRYAQSNLQRSSISP 306
            +Q+Q     +P      +         +  AP      ++P   D   +Q + QR S SP
Sbjct: 312  HQEQTGNSSFPYAHEVAEIQEATRRKSSAVAPS-----DKPLGDDPILSQHDSQRFSTSP 371

Query: 307  PRS------FSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRTRSPPLPSSDLVFGGNSH 366
            P S       SR +  +  G   +V S + A K NS    KRTRSPP+   +     NS 
Sbjct: 372  PTSGTKSYTLSRSSDSQFPGQPSSVNSFNNARKTNSSPATKRTRSPPVYPIEEDIPRNSF 431

Query: 367  STCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNKNEHSMADPNKYLSNQSL 426
             + D  E E  A+AKRL RFK EL   A   +     +   NK    +   NK   N SL
Sbjct: 432  PSQDCTEGEEQARAKRLARFKGELEPIADRPVDIQLTKSPVNKTMKPL--DNKQTFN-SL 491

Query: 427  KLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERERKGDLDHYERLDGDRNHSS 486
            + S +   G+ +PDYE  E  S+I+G+CPDMCPESER ERERKGDLDHYER+DGDRN +S
Sbjct: 492  ESSRDALKGDALPDYENSEQPSLIIGVCPDMCPESERGERERKGDLDHYERVDGDRNQTS 551

Query: 487  KLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRM 546
            K LAVKKY RTAEREA LIRPMPIL+ T++YLL+LL +PY+E FLG YNFLWDRMRAIRM
Sbjct: 552  KSLAVKKYTRTAEREAILIRPMPILQNTMEYLLSLLDRPYNENFLGMYNFLWDRMRAIRM 611

Query: 547  DLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELF 606
            DLRMQH+FN++AIT+LEQMIRLHIIAMHELCE++KGEGF+EGFDAHLNIEQMNKTSVELF
Sbjct: 612  DLRMQHIFNQEAITLLEQMIRLHIIAMHELCEYTKGEGFSEGFDAHLNIEQMNKTSVELF 671

Query: 607  QMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKF 666
            QMYDDHRK+GI VP+EKEFRGYYALLKLDKHPGYKVEP+ELSLDLA MTPE+RQT EV F
Sbjct: 672  QMYDDHRKKGITVPTEKEFRGYYALLKLDKHPGYKVEPSELSLDLANMTPEIRQTSEVLF 731

Query: 667  ARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALASLHSGLQNNQGLPIAH 726
            AR+VARACRT NFIAFFRL RKASYLQACLMHAHF+KLRTQALASLHSGLQ NQGLP++ 
Sbjct: 732  ARNVARACRTGNFIAFFRLARKASYLQACLMHAHFSKLRTQALASLHSGLQINQGLPVSD 791

Query: 727  VCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDFATKCSKLVHMKRSRMI 786
            +  WIGMEEEDIE LLEYHGF IKVFEEPYMV+   FL++DKD+ TKCSKLVHMK+SR I
Sbjct: 792  MSNWIGMEEEDIEALLEYHGFSIKVFEEPYMVKNDLFLHADKDYKTKCSKLVHMKKSRTI 851

Query: 787  VNDVSPCSQNENLITGATKEIPLPRIRKES-----QTFSFEKISSPRTVSAKKESSIRVT 846
            V DVS  +  E++ T      PLP +  E+     Q  +  K   P   S KK++S+R+ 
Sbjct: 852  VEDVSAPTVVEDVST----PFPLPSLITEATIGNQQCITAHKHEMPPARSLKKQTSMRLF 911

Query: 847  NEEMTEF-------DDQP--------------TPVDHKQVQPMIE--------------- 906
            ++E+ +        +D+P               PV H++ Q  +                
Sbjct: 912  DKEVADSKTSLLAEEDKPMGTFVMNPPGPFVINPVVHQEKQNDLTSAGGFHSPVKLYSPF 971

Query: 907  -TPKVSQLHEYNHEV---DGALVQQSGLRSCEPLKTEIKF-----VGNQNYDGLFTTSSM 966
             +PK  Q    N E    D  +    G         EIKF     V   +  G     S 
Sbjct: 972  GSPKFPQTKSSNLEKQPNDDRIGMSPG---------EIKFSIIGDVYTNHVPGPALQQSP 1031

Query: 967  KNISTGIGVSLPLVTEASLQNISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQD 1026
            K++   I   +P+ T A     SV   N  A+E   P+  +   +E +       +  +D
Sbjct: 1032 KSMPMEI---MPVTTIAECPT-SV--ENKYALEESVPEAAMICTLEKEFNDIDEEDEDED 1091

Query: 1027 IVMESCQDEEIANARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLN-NH 1086
             V+ +  DEE+A A+LKLI+RLW+R + ++ +LRE+R LAA AA ++LS+G P++ +   
Sbjct: 1092 GVILNQYDEEVAKAKLKLIIRLWKRWSSRQSELRERRQLAAAAALNSLSLGTPIRFSKTD 1151

Query: 1087 KIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDN 1146
            + R+ G F+ID  +R R++++E SWS +N+SDV+A  +  RN + KCI WK+V+C+Q  +
Sbjct: 1152 QSRACGEFNIDQAMRRRFEEREKSWSRLNISDVIADILVGRNPESKCISWKVVLCTQTKS 1211

Query: 1147 VGDLDQMGQDSRFAAGYWLLSKLMPS------KADDLVLSSSFLSIWRSWISSEIGANLS 1206
            V       Q +  AA  WL SKLMP         D+L+ S+  +S+W  W+++    + +
Sbjct: 1212 VNSSSSASQVTHSAASRWLSSKLMPHAEHSSLNDDNLLFSAPGVSVWNKWVANGSDIDFT 1271

Query: 1207 CFLSIVKDTEFDN-LTETVHGASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLIL 1266
            C LS+ +D E +N + ET  GASA+LF+A+  +P  LQR QL+ +L S+P+GS LPLL++
Sbjct: 1272 CCLSVARDVEAENDMCETTCGASAVLFLASGGLPLNLQREQLNLILESVPNGSVLPLLVV 1331

Query: 1267 --SDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHL-RHLGFFSDEKLKEGLK 1326
              S + + +   + +V+ LGL+DIDKS+I SF +VS+       + + FF+D +L++G K
Sbjct: 1332 ISSCNGEHMEPDTDIVSGLGLHDIDKSKIASFSIVSIANKSQKGQEVHFFNDSRLRDGFK 1391

Query: 1327 WLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDV 1386
            WLA+ SP QP LH VK+  L +TH + S+E+L  M + +V PN CISAFN AL+ S  ++
Sbjct: 1392 WLASNSPLQPNLHHVKLRELFLTHFSFSLELLKQMPDQEVGPNICISAFNDALETSRRNI 1451

Query: 1387 TAAAKENPSNWPCPEIALLE-STDEHTITTHALPPVGWSSVENVEPLRQALMDLKLPSF- 1446
            T+AA+ NP  WPCPE  LLE +  E  +    LP + WSS ENVE L   L + KLP F 
Sbjct: 1452 TSAAEANPIGWPCPETMLLEDNRKECLMVKRYLPNLDWSSAENVELLSSVLENCKLPDFE 1511

Query: 1447 PDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLEL- 1506
             D++WL+ G     EI NH   LE CLI YL Q S +MG  LA +E  +ML+++ +LEL 
Sbjct: 1512 DDLTWLTVGCASGAEIENHTQRLEGCLIEYLTQRSNLMGVSLATKETGVMLERNTRLELH 1571

Query: 1507 QKFKYFIVPDWVTIFRRIFNWRLRYL---AGRSSYVHIVDCYHSVSPTSGVKL-ERREPP 1566
               +Y I P W+ IF+RIFNWR+  L   +  S+YV   D   S S  +   L E    P
Sbjct: 1572 NSSRYHITPRWIGIFQRIFNWRIMGLFDASSSSAYVLKSDLNMSTSSYADKFLAEDASYP 1631

Query: 1567 SYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAIDFSDNDRD 1571
            S  P+ PLL E+IE +CS L    ++  P   +    + +     +   +     + +R+
Sbjct: 1632 SCPPNLPLLHEMIEISCSPL----KSPPPYDDKAQRVVETGMLIDDHRDIEESMLEKNRE 1691

BLAST of MC05g_new0080 vs. ExPASy Swiss-Prot
Match: Q67XV2 (SAC3 family protein C OS=Arabidopsis thaliana OX=3702 GN=SAC3C PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.3e-43
Identity = 113/298 (37.92%), Postives = 174/298 (58.39%), Query Frame = 0

Query: 398 SIIVGLCPDMCPESERAERERKGDLDHYERLDGDRNHSSKLLAVKKYNRT---AEREANL 457
           S+IVG C  MCPE ER  RER  DL  +ERL G+ + SS  +AVKK+ RT   A+ +A+ 
Sbjct: 68  SLIVGTCSSMCPERERVTRERLRDLAVFERLYGNPSKSSTEIAVKKFCRTLSAADVQASD 127

Query: 458 IRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQ 517
           +RP+P+L +T+ YLL+LL    +  F   ++F++DR R+IR DL +Q+L NE  I + E+
Sbjct: 128 VRPLPVLEETLRYLLSLLDSK-EHPFEVVHDFIFDRTRSIRQDLSIQNLANERVIYLYEE 187

Query: 518 MIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKE 577
           M++ H+I+ HE  +   G   +     HLN+EQ+ KT   L+ +YD +RK   I  +E E
Sbjct: 188 MVKFHVIS-HERLQSCSGTSISSMH--HLNMEQLAKTLTSLYNIYDANRKPDYIYENEAE 247

Query: 578 FRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFF- 637
           FR  Y LL L+   G   EP  LSL   K+T  + ++ E+ F R++ R  R  N+  F  
Sbjct: 248 FRSLYVLLHLNPSSGVMGEP--LSLWFRKLTFALVKSKEICFVRNLLRLYRMGNYKNFLS 307

Query: 638 RLVRKASYLQACLMHAHFAKLRTQALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGL 692
           R   +A+YLQ C+   H  ++R  A+  +++     Q  P+  + + + M+E D+E L
Sbjct: 308 RTASEATYLQYCISEHHIREMRLVAVQYINNVCYKLQPYPLLRLSQNLKMKELDVESL 359

BLAST of MC05g_new0080 vs. ExPASy Swiss-Prot
Match: Q9WUU9 (Germinal-center associated nuclear protein OS=Mus musculus OX=10090 GN=Mcm3ap PE=1 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 3.0e-43
Identity = 108/315 (34.29%), Postives = 169/315 (53.65%), Query Frame = 0

Query: 394 LESSSIIVGLCPDMCPESERAERERKGDLDHYERLDGDRNHSSKLLAVKKYNR-TAEREA 453
           L+ +   VG CPDMCPE ER  RE +  L  +E + G  +      AVK+Y+R +A++E 
Sbjct: 620 LDKARAFVGTCPDMCPEKERYLRETRSQLSVFEVVPG-TDQVDHAAAVKEYSRSSADQEE 679

Query: 454 NL---IRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAI 513
            L   +RP  +L +T+DYL+  +    +      Y+F+W+R R IR D+  QHL +   +
Sbjct: 680 PLPHELRPSAVLSRTMDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTV 739

Query: 514 TMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELFQMYDDHRKRGIIV 573
           +++E+  R HI   H +CE          FDA +N E M K    L +MY D R +G+  
Sbjct: 740 SLIEKCTRFHIHCAHFMCEEP-----MSSFDAKINNENMTKCLQSLKEMYQDLRNKGVFC 799

Query: 574 PSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKFARDVARACRTSNF 633
            SE EF+GY  LL L+K         ++  ++ +  P++R +PEV FA     A  ++NF
Sbjct: 800 ASEAEFQGYNVLLNLNK--------GDILREVQQFHPDVRNSPEVNFAVQAFAALNSNNF 859

Query: 634 IAFFRLVRKASYLQACLMHAHFAKLRTQALASLH----SGLQNNQGLPIAHVCKW-IGME 693
           + FF+LV+ ASYL ACL+H +F ++R  AL +L+       Q +   P+  V +  +  +
Sbjct: 860 VRFFKLVQSASYLNACLLHCYFNQIRKDALRALNVAYTVSTQRSTVFPLDGVVRMLLFRD 919

Query: 694 EEDIEGLLEYHGFLI 700
            E+    L YHG  +
Sbjct: 920 SEEATNFLNYHGLTV 920

BLAST of MC05g_new0080 vs. ExPASy Swiss-Prot
Match: O60318 (Germinal-center associated nuclear protein OS=Homo sapiens OX=9606 GN=MCM3AP PE=1 SV=2)

HSP 1 Score: 174.5 bits (441), Expect = 9.6e-42
Identity = 107/315 (33.97%), Postives = 169/315 (53.65%), Query Frame = 0

Query: 394 LESSSIIVGLCPDMCPESERAERERKGDLDHYERLDGDRNHSSKLLAVKKYNR-TAEREA 453
           L+ +   VG C DMCPE ER  RE +  L  +E + G  +      AVK+Y+R +A++E 
Sbjct: 627 LDKARTFVGTCLDMCPEKERYMRETRSQLSVFEVVPG-TDQVDHAAAVKEYSRSSADQEE 686

Query: 454 NL---IRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAI 513
            L   +RP+P+L +T+DYL+  +    +      Y+F+W+R R IR D+  QHL +   +
Sbjct: 687 PLPHELRPLPVLSRTMDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTV 746

Query: 514 TMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELFQMYDDHRKRGIIV 573
           +++E+  R HI   H +CE          FDA +N E M K    L +MY D R +G+  
Sbjct: 747 SLIEKCTRFHIHCAHFMCEEP-----MSSFDAKINNENMTKCLQSLKEMYQDLRNKGVFC 806

Query: 574 PSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKFARDVARACRTSNF 633
            SE EF+GY  LL L+K         ++  ++ +  P +R + EVKFA     A  ++NF
Sbjct: 807 ASEAEFQGYNVLLSLNK--------GDILREVQQFHPAVRNSSEVKFAVQAFAALNSNNF 866

Query: 634 IAFFRLVRKASYLQACLMHAHFAKLRTQALASLH----SGLQNNQGLPIAHVCKWIGMEE 693
           + FF+LV+ ASYL ACL+H +F+++R  AL +L+       Q +   P+  V + +   +
Sbjct: 867 VRFFKLVQSASYLNACLLHCYFSQIRKDALRALNFAYTVSTQRSTIFPLDGVVRMLLFRD 926

Query: 694 -EDIEGLLEYHGFLI 700
            E+    L  HG  +
Sbjct: 927 CEEATDFLTCHGLTV 927

BLAST of MC05g_new0080 vs. ExPASy Swiss-Prot
Match: O74889 (SAC3 family protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC576.05 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.1e-27
Identity = 131/474 (27.64%), Postives = 223/474 (47.05%), Query Frame = 0

Query: 352 MDERDNGNKNEHSMADPNKYLSNQSLK--------LSGNVADGNMPDYEALESSSIIVGL 411
           ++ +D+  K   S  + N++   +SL+         +G + D   P    L+ +   VG 
Sbjct: 50  VEAQDSRQKRFSSTLEGNRFEELRSLREKEREVAIQNGLIDDPTKP--RQLDEAVTFVGT 109

Query: 412 CPDMCPESERAERERKGDLDHYERLDGDRNHSSKLLAVKKYNRTAEREANL----IRPMP 471
           CPDMCPE ER +RE + +L+ +E ++ +     K LAVK ++R A          +RP P
Sbjct: 110 CPDMCPEYEREQREYQNNLERWE-INPETGRVDKNLAVKAFHRPAAGNEQALPSDVRPPP 169

Query: 472 ILRKTIDYLLN-LLSQPYDEKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRL 531
           +L+K++DYL++ ++  P  +    T+ F+ DR R+IR D  +Q+  + DA+   E++ R 
Sbjct: 170 VLKKSLDYLVDKIVCGP--DPLENTHFFVRDRTRSIRQDFTLQNCRDLDAVACHERIARY 229

Query: 532 HIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVE-LFQMYDDHRKRGIIVPSEKEFRG 591
           HI+ +H+LCE        + F A   +EQ+ K  ++ L + YDD RK  I  P+E EFR 
Sbjct: 230 HILCIHQLCE-------KKQFSAQQEVEQLRKGILQSLCEFYDDLRKVKIRCPNEPEFRS 289

Query: 592 YYALLKL---DKHPGYKVEPAEL----SLDLAKMTPEMRQTPEVKFARDVAR---ACRTS 651
           Y  +  L   D     ++ P E+     + LA     + Q    +    + R   AC  +
Sbjct: 290 YAIITHLRDPDVVRQSQILPIEIFDDQRVQLALRLSALAQKNNERVGHILPRNTEAC-PN 349

Query: 652 NFIAFFRLVRK--ASYLQACLMHAHFAKLRTQALASLHSGLQN-NQGLPIAHVCKWIGME 711
            +  FF+LV+    +YL ACL+ +HF  +R  AL ++     + +   P   + + +  +
Sbjct: 350 LYTRFFKLVQSPAVTYLMACLLESHFMSIRKGALKAMRKAFMSAHANFPCGDLKRILHFD 409

Query: 712 E-EDIEGLLEYHGFLIK--VFEEPYMVREGPFLNSDK-DFATKCSK-LVHMK-RSRMIVN 771
             E       Y+G  +     E    + +  F N  K DF    S+ LV  K ++R   +
Sbjct: 410 TVEQAASFSRYYGLEVSDDNGELSINLNKTAFFNDSKPDFRQLFSQTLVESKLQNRSFAD 469

Query: 772 DVSPCSQNENLI---TGATKEIP--LPRIRKESQTFSFEKISSPRTVSAKKESS 788
            ++    N + +   T  +  IP  LP   KE Q  +  K ++P T    K  S
Sbjct: 470 IINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPIAGFKKNTPETSVVSKNLS 510

BLAST of MC05g_new0080 vs. NCBI nr
Match: XP_022137840.1 (SAC3 family protein B isoform X1 [Momordica charantia])

HSP 1 Score: 3094 bits (8021), Expect = 0.0
Identity = 1570/1570 (100.00%), Postives = 1570/1570 (100.00%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60
            MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60

Query: 61   TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE 120
            TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE
Sbjct: 61   TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE 120

Query: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180
            QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH
Sbjct: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180

Query: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240
            PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS
Sbjct: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240

Query: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300
            PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT
Sbjct: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300

Query: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360
            RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK
Sbjct: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360

Query: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420
            NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG
Sbjct: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420

Query: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480
            DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF
Sbjct: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480

Query: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540
            LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD
Sbjct: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540

Query: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600
            AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD
Sbjct: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600

Query: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660
            LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA
Sbjct: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660

Query: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720
            SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF
Sbjct: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720

Query: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780
            ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV
Sbjct: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780

Query: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840
            SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR
Sbjct: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840

Query: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900
            SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER
Sbjct: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900

Query: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960
            VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR
Sbjct: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960

Query: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020
            EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA
Sbjct: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020

Query: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080
            STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS
Sbjct: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080

Query: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140
            SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL
Sbjct: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140

Query: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200
            HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR
Sbjct: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200

Query: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260
            HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC
Sbjct: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260

Query: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320
            ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP
Sbjct: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320

Query: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380
            LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA
Sbjct: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380

Query: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440
            HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV
Sbjct: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440

Query: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500
            KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI
Sbjct: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500

Query: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1560
            DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD
Sbjct: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1560

Query: 1561 AIEKKLSIYF 1570
            AIEKKLSIYF
Sbjct: 1561 AIEKKLSIYF 1570

BLAST of MC05g_new0080 vs. NCBI nr
Match: XP_022137841.1 (SAC3 family protein B isoform X2 [Momordica charantia])

HSP 1 Score: 3011 bits (7805), Expect = 0.0
Identity = 1535/1570 (97.77%), Postives = 1535/1570 (97.77%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60
            MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60

Query: 61   TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE 120
            TTGAAPSLHHQYDR                                   PILFPTRSVEE
Sbjct: 61   TTGAAPSLHHQYDR-----------------------------------PILFPTRSVEE 120

Query: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180
            QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH
Sbjct: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180

Query: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240
            PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS
Sbjct: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240

Query: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300
            PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT
Sbjct: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300

Query: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360
            RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK
Sbjct: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360

Query: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420
            NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG
Sbjct: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420

Query: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480
            DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF
Sbjct: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480

Query: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540
            LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD
Sbjct: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540

Query: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600
            AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD
Sbjct: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600

Query: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660
            LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA
Sbjct: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660

Query: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720
            SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF
Sbjct: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720

Query: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780
            ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV
Sbjct: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780

Query: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840
            SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR
Sbjct: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840

Query: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900
            SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER
Sbjct: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900

Query: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960
            VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR
Sbjct: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960

Query: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020
            EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA
Sbjct: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020

Query: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080
            STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS
Sbjct: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080

Query: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140
            SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL
Sbjct: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140

Query: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200
            HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR
Sbjct: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200

Query: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260
            HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC
Sbjct: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260

Query: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320
            ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP
Sbjct: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320

Query: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380
            LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA
Sbjct: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380

Query: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440
            HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV
Sbjct: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440

Query: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500
            KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI
Sbjct: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500

Query: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1560
            DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD
Sbjct: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1535

Query: 1561 AIEKKLSIYF 1570
            AIEKKLSIYF
Sbjct: 1561 AIEKKLSIYF 1535

BLAST of MC05g_new0080 vs. NCBI nr
Match: XP_038891498.1 (SAC3 family protein B isoform X1 [Benincasa hispida])

HSP 1 Score: 2094 bits (5426), Expect = 0.0
Identity = 1119/1592 (70.29%), Postives = 1236/1592 (77.64%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRA--PRVQSHPMA 60
            MSYQGFGKASGPSAPPKLQHSFG+SA P SVSP         D+S        VQSH MA
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGNSAIPDSVSP-------LRDSSQHEVLGSIVQSHSMA 60

Query: 61   FQTTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSV 120
            FQTTG A +LHHQY                                              
Sbjct: 61   FQTTGTASNLHHQY---------------------------------------------- 120

Query: 121  EEQHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIA 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  GHPVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGN 240
                  RP S S TF++QPK+RGI  NS +YQ + PS  SYD DTNILT  GNVQAPK  
Sbjct: 181  -----HRPVSPSPTFEDQPKVRGILPNSQAYQVRSPSGGSYDHDTNILTESGNVQAPKRT 240

Query: 241  RSPEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPK 300
            +SPE+P  S LRYAQ+NLQR S SPPRSFSR N HEV GSM+N+ +ES  T   SV VPK
Sbjct: 241  KSPEKPFVS-LRYAQTNLQRPSTSPPRSFSRTNTHEVVGSMRNIDAESVGTGSPSVPVPK 300

Query: 301  RTRSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNG 360
            RTRS  LPSSD V GGNSH T DD ERERLAKAKRL RFKDEL E   + LGS+D RDN 
Sbjct: 301  RTRSLALPSSDQVSGGNSHPTHDDTERERLAKAKRLARFKDELDEVTHNKLGSVDVRDNT 360

Query: 361  NKNEHSMADPNKYLSNQSLKLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERE 420
            N+N  S  D +KY+S+QSL+ S N+A GN MPDYEALESSSII+GLCPDMCPESER ERE
Sbjct: 361  NRNGQSTTDRDKYMSSQSLESSRNLAHGNSMPDYEALESSSIIIGLCPDMCPESERGERE 420

Query: 421  RKGDLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYD 480
            RKGDLDHYERLDGDRN +SKLLAVKKY RTAEREANLIRPMP+L KTIDYLL+LLSQPYD
Sbjct: 421  RKGDLDHYERLDGDRNQTSKLLAVKKYTRTAEREANLIRPMPVLLKTIDYLLDLLSQPYD 480

Query: 481  EKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540
            EKFLG YNFLWDRMRAIRMDLRMQHLFNE AITMLEQMIRLHIIAMHELCEFSKGEGFAE
Sbjct: 481  EKFLGIYNFLWDRMRAIRMDLRMQHLFNEKAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540

Query: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600
            GFDAHLNIEQMNKTSVELFQMY+DHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL
Sbjct: 541  GFDAHLNIEQMNKTSVELFQMYEDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600

Query: 601  SLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660
            SLDLAKMTPEMRQT EVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ
Sbjct: 601  SLDLAKMTPEMRQTAEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660

Query: 661  ALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSD 720
            ALASLHSG+QNNQGLPIAHV KWIGMEEEDIEGLLEYHGF IKVFEEPYMVREGPFL+SD
Sbjct: 661  ALASLHSGVQNNQGLPIAHVRKWIGMEEEDIEGLLEYHGFSIKVFEEPYMVREGPFLSSD 720

Query: 721  KDFATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATK-------------------EIP 780
            KDFATKCSKLVH+KRS MIVNDVSP S+NE LI+GATK                   +IP
Sbjct: 721  KDFATKCSKLVHLKRSNMIVNDVSPKSKNEYLISGATKKIPLTKKSKSEYLIPGATKQIP 780

Query: 781  LPRIRKESQTFSFEKISSPRTVSAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPK 840
            L R +KES+TFSF KI SPR ++ +KESS+   +EEMTEFDDQ  P DHKQVQP+IE  +
Sbjct: 781  LTRTKKESKTFSFGKIGSPRPIATEKESSVHEIDEEMTEFDDQSVPADHKQVQPIIEMSE 840

Query: 841  VSQLHEYNHEVDGALVQQSGLRSCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLP 900
            V QLHEYNHE +GAL+Q SG RSCEP +TE+KFVGNQ YDGLF TS  +N S  IG+SLP
Sbjct: 841  VCQLHEYNHEANGALLQ-SGPRSCEPSRTEVKFVGNQGYDGLFMTSPARNNSARIGLSLP 900

Query: 901  LVTEASLQNISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIA 960
            LV++AS Q I  CGYND+ +   +P+ +VNNVMED+E L A+ ENK DIV+ESC DEEIA
Sbjct: 901  LVSDASHQKIPTCGYNDNTIRSAKPESIVNNVMEDEEILNATQENKHDIVIESCPDEEIA 960

Query: 961  NARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHT 1020
            +ARLKLILRLWRRRALK+KQLR+QR+LAA+AAF+TLSVGPP+QLNNHKI+SIG+FDIDH 
Sbjct: 961  DARLKLILRLWRRRALKRKQLRQQRLLAAEAAFNTLSVGPPIQLNNHKIKSIGIFDIDHI 1020

Query: 1021 LRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRF 1080
            +RERWK+Q+LSWS+VNVS+VVAS +SRRNV+ KCICWKLV+CSQ           +DS F
Sbjct: 1021 VRERWKRQKLSWSVVNVSEVVASILSRRNVEEKCICWKLVVCSQRT---------EDSHF 1080

Query: 1081 AAGYWLLSKLMPSKADDLVLSSSFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVH 1140
            AAG WLLSKLMPSKA+DL+ SSSFLSIW+S +  E G +LSCFLSIV+   F NL ETVH
Sbjct: 1081 AAGSWLLSKLMPSKANDLIFSSSFLSIWKSCLLDETGVDLSCFLSIVRHANFGNLPETVH 1140

Query: 1141 GASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLY 1200
            GASA+LFVATESIP +LQRVQLHKLL SIPSGSCLPLLILSD HDEVSAS  L NKL LY
Sbjct: 1141 GASAVLFVATESIPLDLQRVQLHKLLASIPSGSCLPLLILSDFHDEVSAS--LANKLDLY 1200

Query: 1201 DIDKSRIHSFQVVSLLENPHLRHLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIIT 1260
            DIDKSRIHSFQVVSLL+NPHLRHLGFFSDEKLKEGLKWLANES  QPVLHRVKVL+LII+
Sbjct: 1201 DIDKSRIHSFQVVSLLDNPHLRHLGFFSDEKLKEGLKWLANESLSQPVLHRVKVLDLIIS 1260

Query: 1261 HLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTD 1320
            HL+ S+E+LDSM E DVSPN CISAFNLALDQS+ D+TAA K NPSNWPCPEIALLES +
Sbjct: 1261 HLDLSMEVLDSMNEKDVSPNHCISAFNLALDQSVADITAAVKANPSNWPCPEIALLESCN 1320

Query: 1321 EHTITTHALPPVGWSSVENVEPLRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLES 1380
            E    T ALPPVGWS VENVEPL+QALM LKLP+FPD+SWL+K SN + EIP  RD LES
Sbjct: 1321 EPAFMTDALPPVGWSLVENVEPLKQALMGLKLPTFPDISWLTKCSNTIKEIPTVRDNLES 1380

Query: 1381 CLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYL 1440
            CL  YL QTSEIMGQQLA+EEAHIMLQK AKLEL  F YFIVP W TIFRRIF+WRLRY 
Sbjct: 1381 CLRCYLTQTSEIMGQQLALEEAHIMLQKCAKLELHNFNYFIVPHWGTIFRRIFSWRLRYF 1440

Query: 1441 AGRSSYVHIVDCYHSVSPTSGVKLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPD 1500
              RSSYVHIV+C H  S +S V+LE RE PSYRP+QPLLDE+IE ACSSLSIDQ     +
Sbjct: 1441 PSRSSYVHIVNCCHGASVSSSVRLESRERPSYRPNQPLLDEVIEVACSSLSIDQERHFSE 1461

Query: 1501 AHQPLAAMTSNSRPPEVAAVAIDFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVAS 1560
            AHQPLA +TSNSRP EV   AIDF+++D +STR+IGFVSSE +PNLG+ L+CTGKELV S
Sbjct: 1501 AHQPLATITSNSRPCEVVIRAIDFANDDSNSTRQIGFVSSESLPNLGRELTCTGKELVIS 1461

Query: 1561 STVYSEAVRLNELLDRCNELQDAIEKKLSIYF 1570
             T YSEA RL ELLD+CN+ QDAIEK LSIYF
Sbjct: 1561 GTGYSEAARLKELLDQCNKRQDAIEKMLSIYF 1461

BLAST of MC05g_new0080 vs. NCBI nr
Match: XP_022955623.1 (SAC3 family protein B [Cucurbita moschata])

HSP 1 Score: 2090 bits (5414), Expect = 0.0
Identity = 1119/1598 (70.03%), Postives = 1241/1598 (77.66%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSP--SPSTFPAFSDASPPRAPRVQSHPMA 60
            MSYQGFGKASGPSAPPKLQHSFG+SA P SVSP  + S   AF         RVQSHPMA
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGNSAIPDSVSPLRASSHHEAFGS-------RVQSHPMA 60

Query: 61   FQTTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSV 120
            FQT G A SLHHQY                                              
Sbjct: 61   FQTPGTASSLHHQY---------------------------------------------- 120

Query: 121  EEQHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIA 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  GHPVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGN 240
                  RP SSS  F+ QPK+RGI  NS +YQD+   +RS+D DTNI       QAPK  
Sbjct: 181  -----HRPVSSSPIFEEQPKVRGILPNSQAYQDRALPERSHDHDTNI-------QAPKRT 240

Query: 241  RSPEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPK 300
            +SPE+P  S+LR AQ+NL R S SPPR FS  N  E  GSM+N+ +ES  T+P  VLV K
Sbjct: 241  KSPEKPFVSNLRSAQTNLLRLSTSPPRPFSWSNTQEAVGSMRNINAESVVTEPTGVLVSK 300

Query: 301  RTRSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNG 360
            RT+SP L SSD V GGNS  T DD ERERLAKAKRL RFK ELVE A S LGSMD RDN 
Sbjct: 301  RTKSPTLSSSDQVSGGNSDPTPDDTERERLAKAKRLARFKVELVEVAHSKLGSMDARDNS 360

Query: 361  NKNEHSMADPNKYLSNQSLKLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERE 420
            N+NEHS  + +K +SNQSL+ S N+A GN MPDYEALESSSII+GLCPDMCPESERAERE
Sbjct: 361  NRNEHSTTERDKCMSNQSLESSTNLAQGNSMPDYEALESSSIIIGLCPDMCPESERAERE 420

Query: 421  RKGDLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYD 480
            RKGDLDHYERLDGDRN +SKLLAVKKY RTAEREANLIRPMP+L KTI+YLL LLSQPYD
Sbjct: 421  RKGDLDHYERLDGDRNQTSKLLAVKKYTRTAEREANLIRPMPVLLKTIEYLLELLSQPYD 480

Query: 481  EKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540
            EKFLG YNFLWDRMRAIRMDLRMQHLFNE+AITMLEQMIRLHIIAMHELCEFSKGEGFAE
Sbjct: 481  EKFLGIYNFLWDRMRAIRMDLRMQHLFNENAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540

Query: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600
            GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL
Sbjct: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600

Query: 601  SLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660
            SLDLAKMTPEMRQT EVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ
Sbjct: 601  SLDLAKMTPEMRQTAEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660

Query: 661  ALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSD 720
            ALASLHSG+QNNQGLPI+HV KWIGMEEEDIEGLLEYHGF IKVFEEPYMVREGPFLNSD
Sbjct: 661  ALASLHSGVQNNQGLPISHVRKWIGMEEEDIEGLLEYHGFSIKVFEEPYMVREGPFLNSD 720

Query: 721  KDFATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLP----------------- 780
            KDFATKCSKLVHMKRS MIV DVSP S+NE LITGATK+ PL                  
Sbjct: 721  KDFATKCSKLVHMKRSGMIVTDVSPKSRNEYLITGATKQTPLTWKSKNEYLSTSAIKQIP 780

Query: 781  --RIRKESQTFSFEKISSPRTVSAKKE--SSIRVTNEEMTEFDDQPTPVDHKQVQPM--- 840
                +K+   FSFEKISS R VS +KE  SS+   +E+M EFDD   P+D KQVQPM   
Sbjct: 781  SKMTKKQLPIFSFEKISSSRPVSTEKEKESSVHEIDEDMVEFDDPLIPIDPKQVQPMQPM 840

Query: 841  IETPKVSQLHEYNHEVDGALVQQSGLRSCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGI 900
            IET +V QLHEYNH V+GAL+ QSG RSCEPL+TE+KFVGNQ YDG+F TS ++NISTG+
Sbjct: 841  IETSEVGQLHEYNHGVNGALLLQSGPRSCEPLRTEVKFVGNQCYDGVFVTSPVRNISTGM 900

Query: 901  GVSLPLVTEASLQNISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQDIVMESCQ 960
            G+SLPLV++A  Q ISVCGYND+A+  V+PQ +VNN+MED+E LYA+ ENK DIV E C 
Sbjct: 901  GMSLPLVSDAPPQKISVCGYNDNAIGSVKPQSIVNNMMEDEEILYATQENKHDIVTEGCP 960

Query: 961  DEEIANARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVF 1020
            DEEIA+ARLKLILRLWRRRA+K+KQLREQR+LAAKAAF TLSVGPPVQL NHKIRS+GVF
Sbjct: 961  DEEIADARLKLILRLWRRRAVKRKQLREQRLLAAKAAFDTLSVGPPVQLKNHKIRSVGVF 1020

Query: 1021 DIDHTLRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDNVGDLDQMG 1080
            DIDH LRERWK+Q+LS S+VNVS+VVAS++S +NVD KCICWKLV+CSQMD+ GDL Q  
Sbjct: 1021 DIDHILRERWKRQKLSCSVVNVSEVVASSLSGKNVDGKCICWKLVVCSQMDSAGDLVQGT 1080

Query: 1081 QDSRFAAGYWLLSKLMPSKADDLVLSSSFLSIWRSWISSEIGANLSCFLSIVKDTEFDNL 1140
             DS  +AG WLLSKLMPS+A+DLV SSSFLSIW+SW+S + G + SCFLS+VK   FDNL
Sbjct: 1081 GDSHISAGSWLLSKLMPSEANDLVFSSSFLSIWKSWLSGKTGVDSSCFLSVVKHANFDNL 1140

Query: 1141 TETVHGASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLILSDSHDEVSASSMLVN 1200
             ETV GASAILFVAT+S P +LQRVQLHKLL+SIPSGSCLPLLILSD HDE SASSMLVN
Sbjct: 1141 PETVRGASAILFVATQSTPLDLQRVQLHKLLLSIPSGSCLPLLILSDLHDEASASSMLVN 1200

Query: 1201 KLGLYDIDKSRIHSFQVVSLLENPHLRHLGFFSDEKLKEGLKWLANESPPQPVLHRVKVL 1260
            KLGLYDIDKSRI SFQVVSLL+NPHLRHLGFFSDEKLKEGLKWLANESP QPVLHRVKVL
Sbjct: 1201 KLGLYDIDKSRICSFQVVSLLDNPHLRHLGFFSDEKLKEGLKWLANESPSQPVLHRVKVL 1260

Query: 1261 NLIITHLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDVTAAAKENPSNWPCPEIAL 1320
            +LIITHL+ S+EMLDSM E DVSPNQCISAFNLALDQSL D+TAA K NPSNWPCPEIA 
Sbjct: 1261 DLIITHLDLSMEMLDSMNEKDVSPNQCISAFNLALDQSLADITAAVKANPSNWPCPEIAS 1320

Query: 1321 LESTDEHTITTHALPPVGWSSVENVEPLRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHR 1380
            L S +EH I T ALPPVGWSS ENVEPL++ALMDLKLP+FPD+SWL+KGSNM  EIP  R
Sbjct: 1321 LASCNEHGIITDALPPVGWSSAENVEPLKKALMDLKLPTFPDISWLTKGSNMAKEIPTLR 1380

Query: 1381 DYLESCLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNW 1440
            D LE+CL  YL QTS+IM QQLAMEEAHIMLQK AKLEL KF YFIVP WV IFRRI NW
Sbjct: 1381 DNLENCLSCYLTQTSKIMEQQLAMEEAHIMLQKCAKLELHKFCYFIVPHWVKIFRRISNW 1440

Query: 1441 RLRYLAGRSSYVHIVDCYHSVSPTSGVKLERREPPSYRPDQPLLDELIEAACSSLSIDQR 1500
            RLRY +GRSSYV+IVDC H  S  S V+LE REPPSY P+QPLLDE+IE ACSSLSID+ 
Sbjct: 1441 RLRYFSGRSSYVNIVDCCHGASAVSSVRLESREPPSYTPNQPLLDEVIEVACSSLSIDEG 1473

Query: 1501 TQLPDAHQPLAAMTSNSRPPEVAAVAIDFSDNDRDSTREIGFVSSEC-VPNLGQRLSCTG 1560
              L +AHQPLAA+TSNSRP E+    I+F+D+  ++TR++GFVSS+  VPN G+ L+CTG
Sbjct: 1501 RPLSEAHQPLAAITSNSRPRELVVTTINFADDGSNNTRQVGFVSSDSIVPNSGRELNCTG 1473

Query: 1561 KELVASSTVYSEAVRLNELLDRCNELQDAIEKKLSIYF 1570
            KE+VA+ T YSEA RL +LLD+C++ QDAIEK LSIY+
Sbjct: 1561 KEVVANDTRYSEAARLKKLLDQCDKRQDAIEKVLSIYY 1473

BLAST of MC05g_new0080 vs. NCBI nr
Match: KAG6582261.1 (SAC3 family protein B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2088 bits (5409), Expect = 0.0
Identity = 1118/1600 (69.88%), Postives = 1241/1600 (77.56%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSP--SPSTFPAFSDASPPRAPRVQSHPMA 60
            MSYQGFGKASGPSAPPKLQHSFG+SA P SVSP  + S   AF         RVQSHPMA
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGNSAIPDSVSPLRASSQHEAFGS-------RVQSHPMA 60

Query: 61   FQTTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSV 120
            FQT G A SLHHQY                                              
Sbjct: 61   FQTPGTASSLHHQY---------------------------------------------- 120

Query: 121  EEQHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIA 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  GHPVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGN 240
                  RP SSS  F+ QPK+RGI  NS +YQD+   +RS+D DTNI       QAPK  
Sbjct: 181  -----HRPVSSSPIFEEQPKVRGILPNSQAYQDRALPERSHDHDTNI-------QAPKRT 240

Query: 241  RSPEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPK 300
            +SPE+P  S+LR AQ+NL R S SPPR FS  N  E  GSM+N+ +ES  T+P  VLV K
Sbjct: 241  KSPEKPFVSNLRSAQTNLLRLSTSPPRPFSWSNTQEAVGSMRNINAESVVTEPTGVLVSK 300

Query: 301  RTRSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNG 360
            RT+SP L SSD V GGNS  T DD ERERLAKAKRL RFK ELVE A S LGSMD RDN 
Sbjct: 301  RTKSPTLSSSDQVSGGNSDPTPDDTERERLAKAKRLARFKVELVEVAHSKLGSMDARDNS 360

Query: 361  NKNEHSMADPNKYLSNQSLKLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERE 420
            N+NEHS  + +K +SNQSL+ S N+A GN MPDYEALESSSII+GLCPDMCPESERAERE
Sbjct: 361  NRNEHSTTERDKCMSNQSLESSTNLAQGNSMPDYEALESSSIIIGLCPDMCPESERAERE 420

Query: 421  RKGDLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYD 480
            RKGDLDHYERLDGDRN +SKLLAVKKY RTAEREANLIRPMP+L KTI+YLL LLSQPYD
Sbjct: 421  RKGDLDHYERLDGDRNQTSKLLAVKKYTRTAEREANLIRPMPVLLKTIEYLLELLSQPYD 480

Query: 481  EKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540
            EKFLG YNFLWDRMRAIRMDLRMQHLFNE+AITMLEQMIRLHIIAMHELCEFSKGEGFAE
Sbjct: 481  EKFLGIYNFLWDRMRAIRMDLRMQHLFNENAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540

Query: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600
            GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL
Sbjct: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600

Query: 601  SLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660
            SLDLAKMTPEMRQT EVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ
Sbjct: 601  SLDLAKMTPEMRQTAEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660

Query: 661  ALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSD 720
            ALASLHSG+QNNQGLPI+HV KWIGMEEEDIEGLLEYHGF IKVFEEPYMVREGPFLNSD
Sbjct: 661  ALASLHSGVQNNQGLPISHVRKWIGMEEEDIEGLLEYHGFSIKVFEEPYMVREGPFLNSD 720

Query: 721  KDFATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLP----------------- 780
            KDFATKCSKLVHMKRS MIV DVSP S+NE LITGATK+ PL                  
Sbjct: 721  KDFATKCSKLVHMKRSGMIVTDVSPKSRNEYLITGATKQTPLTWKSKNEYLSTSAIKQIP 780

Query: 781  --RIRKESQTFSFEKISSPRTVSAKKE----SSIRVTNEEMTEFDDQPTPVDHKQVQPM- 840
                +K+   FSFEKISS R VS +KE    SS+   +E+M EFDD   P+D KQVQPM 
Sbjct: 781  SKMTKKQLPIFSFEKISSSRPVSTEKEKEKESSVHEIDEDMVEFDDPLIPIDPKQVQPMQ 840

Query: 841  --IETPKVSQLHEYNHEVDGALVQQSGLRSCEPLKTEIKFVGNQNYDGLFTTSSMKNIST 900
              IET +V QLHEYNH V+GAL+ QSG RSCEPL+TE+KFVGNQ +DG+F TS ++NIST
Sbjct: 841  PMIETSEVGQLHEYNHGVNGALLLQSGPRSCEPLRTEVKFVGNQCFDGVFVTSPVRNIST 900

Query: 901  GIGVSLPLVTEASLQNISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQDIVMES 960
            G+G+SLPLV++A  Q ISVCGYND+A+  V+PQ +VNN+MED+E LYA+ ENK DIV E 
Sbjct: 901  GMGMSLPLVSDAPPQKISVCGYNDNAIGSVKPQSIVNNMMEDEEILYATQENKHDIVTEG 960

Query: 961  CQDEEIANARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLNNHKIRSIG 1020
            C DEEIA+ARLKLILRLWRRRA+K+KQLREQR+LAAKAAF TLSVGPPVQL NHKIRS+G
Sbjct: 961  CPDEEIADARLKLILRLWRRRAVKRKQLREQRLLAAKAAFDTLSVGPPVQLKNHKIRSVG 1020

Query: 1021 VFDIDHTLRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDNVGDLDQ 1080
            VFDIDH LRERWK+Q+LS S+VNVS+VVAS++S +NVD KCICWKLV+CSQMD+ GDL Q
Sbjct: 1021 VFDIDHILRERWKRQKLSCSVVNVSEVVASSLSGKNVDGKCICWKLVVCSQMDSAGDLVQ 1080

Query: 1081 MGQDSRFAAGYWLLSKLMPSKADDLVLSSSFLSIWRSWISSEIGANLSCFLSIVKDTEFD 1140
               DS  +AG WLLSKLMPS+A+DLV SSSFLSIW+SW+S + G + SCFLS+VK   FD
Sbjct: 1081 GTGDSHISAGSWLLSKLMPSEANDLVFSSSFLSIWKSWLSGKTGVDSSCFLSVVKHANFD 1140

Query: 1141 NLTETVHGASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLILSDSHDEVSASSML 1200
            NL ETV GASAILFVAT+S P +LQRVQLHKLL+SIPSGSCLPLLILSD HDE SASSML
Sbjct: 1141 NLPETVRGASAILFVATQSTPLDLQRVQLHKLLLSIPSGSCLPLLILSDLHDEASASSML 1200

Query: 1201 VNKLGLYDIDKSRIHSFQVVSLLENPHLRHLGFFSDEKLKEGLKWLANESPPQPVLHRVK 1260
            VNKLGLYDIDKSRI SFQVVSLL+NPHLRHLGFFSDEKLKEGLKWLANESP QPVLHRVK
Sbjct: 1201 VNKLGLYDIDKSRICSFQVVSLLDNPHLRHLGFFSDEKLKEGLKWLANESPSQPVLHRVK 1260

Query: 1261 VLNLIITHLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDVTAAAKENPSNWPCPEI 1320
            VL+LIITHL+ S+EMLDSM E DVSPNQCISAFNLALDQSL D+TAA K NPSNWPCPEI
Sbjct: 1261 VLDLIITHLDLSMEMLDSMNEKDVSPNQCISAFNLALDQSLADITAAVKANPSNWPCPEI 1320

Query: 1321 ALLESTDEHTITTHALPPVGWSSVENVEPLRQALMDLKLPSFPDMSWLSKGSNMVNEIPN 1380
            A L S +EH I T ALPPVGWSS ENVEPL++ALMDLKLP+FPD+SWL+KGSNM  EIP 
Sbjct: 1321 ASLASCNEHGIITDALPPVGWSSAENVEPLKKALMDLKLPTFPDISWLTKGSNMAKEIPT 1380

Query: 1381 HRDYLESCLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLELQKFKYFIVPDWVTIFRRIF 1440
             RD LE+CL  YL QTS+IM QQLAMEEAHIMLQK AKLEL KF YFIVP WV IFRRI 
Sbjct: 1381 LRDNLENCLSCYLTQTSKIMEQQLAMEEAHIMLQKCAKLELHKFCYFIVPHWVKIFRRIS 1440

Query: 1441 NWRLRYLAGRSSYVHIVDCYHSVSPTSGVKLERREPPSYRPDQPLLDELIEAACSSLSID 1500
            NWRLRY +GRSSYV+IVDC H  S  S V+LE REPPSY P+QPLLDE+IE ACSSLSID
Sbjct: 1441 NWRLRYFSGRSSYVNIVDCCHGASAVSSVRLESREPPSYTPNQPLLDEVIEVACSSLSID 1475

Query: 1501 QRTQLPDAHQPLAAMTSNSRPPEVAAVAIDFSDNDRDSTREIGFVSSEC-VPNLGQRLSC 1560
            +   L +AHQPLAA+TSNSRP E+    I+F+D+  ++TR++GFVSS+  VPN G+ L+C
Sbjct: 1501 EGRPLSEAHQPLAAITSNSRPRELVVTTINFADDGSNNTRQVGFVSSDSIVPNSGRELNC 1475

Query: 1561 TGKELVASSTVYSEAVRLNELLDRCNELQDAIEKKLSIYF 1570
            TGKE+VA+ T YSEA RL +LLD+C++ QDAIEK LSIY+
Sbjct: 1561 TGKEVVANDTRYSEAARLKKLLDQCDKRQDAIEKVLSIYY 1475

BLAST of MC05g_new0080 vs. ExPASy TrEMBL
Match: A0A6J1C7T2 (SAC3 family protein B isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009180 PE=4 SV=1)

HSP 1 Score: 3094 bits (8021), Expect = 0.0
Identity = 1570/1570 (100.00%), Postives = 1570/1570 (100.00%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60
            MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60

Query: 61   TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE 120
            TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE
Sbjct: 61   TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE 120

Query: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180
            QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH
Sbjct: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180

Query: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240
            PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS
Sbjct: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240

Query: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300
            PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT
Sbjct: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300

Query: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360
            RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK
Sbjct: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360

Query: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420
            NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG
Sbjct: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420

Query: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480
            DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF
Sbjct: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480

Query: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540
            LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD
Sbjct: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540

Query: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600
            AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD
Sbjct: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600

Query: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660
            LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA
Sbjct: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660

Query: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720
            SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF
Sbjct: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720

Query: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780
            ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV
Sbjct: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780

Query: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840
            SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR
Sbjct: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840

Query: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900
            SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER
Sbjct: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900

Query: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960
            VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR
Sbjct: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960

Query: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020
            EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA
Sbjct: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020

Query: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080
            STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS
Sbjct: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080

Query: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140
            SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL
Sbjct: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140

Query: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200
            HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR
Sbjct: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200

Query: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260
            HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC
Sbjct: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260

Query: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320
            ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP
Sbjct: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320

Query: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380
            LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA
Sbjct: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380

Query: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440
            HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV
Sbjct: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440

Query: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500
            KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI
Sbjct: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500

Query: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1560
            DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD
Sbjct: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1560

Query: 1561 AIEKKLSIYF 1570
            AIEKKLSIYF
Sbjct: 1561 AIEKKLSIYF 1570

BLAST of MC05g_new0080 vs. ExPASy TrEMBL
Match: A0A6J1C7V5 (SAC3 family protein B isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009180 PE=4 SV=1)

HSP 1 Score: 3011 bits (7805), Expect = 0.0
Identity = 1535/1570 (97.77%), Postives = 1535/1570 (97.77%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60
            MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSPSPSTFPAFSDASPPRAPRVQSHPMAFQ 60

Query: 61   TTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSVEE 120
            TTGAAPSLHHQYDR                                   PILFPTRSVEE
Sbjct: 61   TTGAAPSLHHQYDR-----------------------------------PILFPTRSVEE 120

Query: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180
            QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH
Sbjct: 121  QHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIAGH 180

Query: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240
            PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS
Sbjct: 181  PVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRS 240

Query: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300
            PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT
Sbjct: 241  PEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRT 300

Query: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360
            RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK
Sbjct: 301  RSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNK 360

Query: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420
            NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG
Sbjct: 361  NEHSMADPNKYLSNQSLKLSGNVADGNMPDYEALESSSIIVGLCPDMCPESERAERERKG 420

Query: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480
            DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF
Sbjct: 421  DLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKF 480

Query: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540
            LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD
Sbjct: 481  LGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFD 540

Query: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600
            AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD
Sbjct: 541  AHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLD 600

Query: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660
            LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA
Sbjct: 601  LAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALA 660

Query: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720
            SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF
Sbjct: 661  SLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDF 720

Query: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780
            ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV
Sbjct: 721  ATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLPRIRKESQTFSFEKISSPRTV 780

Query: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840
            SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR
Sbjct: 781  SAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHEYNHEVDGALVQQSGLR 840

Query: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900
            SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER
Sbjct: 841  SCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTEASLQNISVCGYNDSAMER 900

Query: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960
            VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR
Sbjct: 901  VEPQILVNNVMEDKETLYASVENKQDIVMESCQDEEIANARLKLILRLWRRRALKKKQLR 960

Query: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020
            EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA
Sbjct: 961  EQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVA 1020

Query: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080
            STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS
Sbjct: 1021 STMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDSRFAAGYWLLSKLMPSKADDLVLSS 1080

Query: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140
            SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL
Sbjct: 1081 SFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTETVHGASAILFVATESIPWELQRVQL 1140

Query: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200
            HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR
Sbjct: 1141 HKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHLR 1200

Query: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260
            HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC
Sbjct: 1201 HLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQC 1260

Query: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320
            ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP
Sbjct: 1261 ISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLESTDEHTITTHALPPVGWSSVENVEP 1320

Query: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380
            LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA
Sbjct: 1321 LRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEA 1380

Query: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440
            HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV
Sbjct: 1381 HIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLRYLAGRSSYVHIVDCYHSVSPTSGV 1440

Query: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500
            KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI
Sbjct: 1441 KLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAI 1500

Query: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1560
            DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD
Sbjct: 1501 DFSDNDRDSTREIGFVSSECVPNLGQRLSCTGKELVASSTVYSEAVRLNELLDRCNELQD 1535

Query: 1561 AIEKKLSIYF 1570
            AIEKKLSIYF
Sbjct: 1561 AIEKKLSIYF 1535

BLAST of MC05g_new0080 vs. ExPASy TrEMBL
Match: A0A6J1GUH5 (SAC3 family protein B OS=Cucurbita moschata OX=3662 GN=LOC111457572 PE=4 SV=1)

HSP 1 Score: 2090 bits (5414), Expect = 0.0
Identity = 1119/1598 (70.03%), Postives = 1241/1598 (77.66%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSP--SPSTFPAFSDASPPRAPRVQSHPMA 60
            MSYQGFGKASGPSAPPKLQHSFG+SA P SVSP  + S   AF         RVQSHPMA
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGNSAIPDSVSPLRASSHHEAFGS-------RVQSHPMA 60

Query: 61   FQTTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSV 120
            FQT G A SLHHQY                                              
Sbjct: 61   FQTPGTASSLHHQY---------------------------------------------- 120

Query: 121  EEQHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIA 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  GHPVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGN 240
                  RP SSS  F+ QPK+RGI  NS +YQD+   +RS+D DTNI       QAPK  
Sbjct: 181  -----HRPVSSSPIFEEQPKVRGILPNSQAYQDRALPERSHDHDTNI-------QAPKRT 240

Query: 241  RSPEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPK 300
            +SPE+P  S+LR AQ+NL R S SPPR FS  N  E  GSM+N+ +ES  T+P  VLV K
Sbjct: 241  KSPEKPFVSNLRSAQTNLLRLSTSPPRPFSWSNTQEAVGSMRNINAESVVTEPTGVLVSK 300

Query: 301  RTRSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNG 360
            RT+SP L SSD V GGNS  T DD ERERLAKAKRL RFK ELVE A S LGSMD RDN 
Sbjct: 301  RTKSPTLSSSDQVSGGNSDPTPDDTERERLAKAKRLARFKVELVEVAHSKLGSMDARDNS 360

Query: 361  NKNEHSMADPNKYLSNQSLKLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERE 420
            N+NEHS  + +K +SNQSL+ S N+A GN MPDYEALESSSII+GLCPDMCPESERAERE
Sbjct: 361  NRNEHSTTERDKCMSNQSLESSTNLAQGNSMPDYEALESSSIIIGLCPDMCPESERAERE 420

Query: 421  RKGDLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYD 480
            RKGDLDHYERLDGDRN +SKLLAVKKY RTAEREANLIRPMP+L KTI+YLL LLSQPYD
Sbjct: 421  RKGDLDHYERLDGDRNQTSKLLAVKKYTRTAEREANLIRPMPVLLKTIEYLLELLSQPYD 480

Query: 481  EKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540
            EKFLG YNFLWDRMRAIRMDLRMQHLFNE+AITMLEQMIRLHIIAMHELCEFSKGEGFAE
Sbjct: 481  EKFLGIYNFLWDRMRAIRMDLRMQHLFNENAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540

Query: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600
            GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL
Sbjct: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600

Query: 601  SLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660
            SLDLAKMTPEMRQT EVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ
Sbjct: 601  SLDLAKMTPEMRQTAEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660

Query: 661  ALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSD 720
            ALASLHSG+QNNQGLPI+HV KWIGMEEEDIEGLLEYHGF IKVFEEPYMVREGPFLNSD
Sbjct: 661  ALASLHSGVQNNQGLPISHVRKWIGMEEEDIEGLLEYHGFSIKVFEEPYMVREGPFLNSD 720

Query: 721  KDFATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLP----------------- 780
            KDFATKCSKLVHMKRS MIV DVSP S+NE LITGATK+ PL                  
Sbjct: 721  KDFATKCSKLVHMKRSGMIVTDVSPKSRNEYLITGATKQTPLTWKSKNEYLSTSAIKQIP 780

Query: 781  --RIRKESQTFSFEKISSPRTVSAKKE--SSIRVTNEEMTEFDDQPTPVDHKQVQPM--- 840
                +K+   FSFEKISS R VS +KE  SS+   +E+M EFDD   P+D KQVQPM   
Sbjct: 781  SKMTKKQLPIFSFEKISSSRPVSTEKEKESSVHEIDEDMVEFDDPLIPIDPKQVQPMQPM 840

Query: 841  IETPKVSQLHEYNHEVDGALVQQSGLRSCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGI 900
            IET +V QLHEYNH V+GAL+ QSG RSCEPL+TE+KFVGNQ YDG+F TS ++NISTG+
Sbjct: 841  IETSEVGQLHEYNHGVNGALLLQSGPRSCEPLRTEVKFVGNQCYDGVFVTSPVRNISTGM 900

Query: 901  GVSLPLVTEASLQNISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQDIVMESCQ 960
            G+SLPLV++A  Q ISVCGYND+A+  V+PQ +VNN+MED+E LYA+ ENK DIV E C 
Sbjct: 901  GMSLPLVSDAPPQKISVCGYNDNAIGSVKPQSIVNNMMEDEEILYATQENKHDIVTEGCP 960

Query: 961  DEEIANARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVF 1020
            DEEIA+ARLKLILRLWRRRA+K+KQLREQR+LAAKAAF TLSVGPPVQL NHKIRS+GVF
Sbjct: 961  DEEIADARLKLILRLWRRRAVKRKQLREQRLLAAKAAFDTLSVGPPVQLKNHKIRSVGVF 1020

Query: 1021 DIDHTLRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDNVGDLDQMG 1080
            DIDH LRERWK+Q+LS S+VNVS+VVAS++S +NVD KCICWKLV+CSQMD+ GDL Q  
Sbjct: 1021 DIDHILRERWKRQKLSCSVVNVSEVVASSLSGKNVDGKCICWKLVVCSQMDSAGDLVQGT 1080

Query: 1081 QDSRFAAGYWLLSKLMPSKADDLVLSSSFLSIWRSWISSEIGANLSCFLSIVKDTEFDNL 1140
             DS  +AG WLLSKLMPS+A+DLV SSSFLSIW+SW+S + G + SCFLS+VK   FDNL
Sbjct: 1081 GDSHISAGSWLLSKLMPSEANDLVFSSSFLSIWKSWLSGKTGVDSSCFLSVVKHANFDNL 1140

Query: 1141 TETVHGASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLILSDSHDEVSASSMLVN 1200
             ETV GASAILFVAT+S P +LQRVQLHKLL+SIPSGSCLPLLILSD HDE SASSMLVN
Sbjct: 1141 PETVRGASAILFVATQSTPLDLQRVQLHKLLLSIPSGSCLPLLILSDLHDEASASSMLVN 1200

Query: 1201 KLGLYDIDKSRIHSFQVVSLLENPHLRHLGFFSDEKLKEGLKWLANESPPQPVLHRVKVL 1260
            KLGLYDIDKSRI SFQVVSLL+NPHLRHLGFFSDEKLKEGLKWLANESP QPVLHRVKVL
Sbjct: 1201 KLGLYDIDKSRICSFQVVSLLDNPHLRHLGFFSDEKLKEGLKWLANESPSQPVLHRVKVL 1260

Query: 1261 NLIITHLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDVTAAAKENPSNWPCPEIAL 1320
            +LIITHL+ S+EMLDSM E DVSPNQCISAFNLALDQSL D+TAA K NPSNWPCPEIA 
Sbjct: 1261 DLIITHLDLSMEMLDSMNEKDVSPNQCISAFNLALDQSLADITAAVKANPSNWPCPEIAS 1320

Query: 1321 LESTDEHTITTHALPPVGWSSVENVEPLRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHR 1380
            L S +EH I T ALPPVGWSS ENVEPL++ALMDLKLP+FPD+SWL+KGSNM  EIP  R
Sbjct: 1321 LASCNEHGIITDALPPVGWSSAENVEPLKKALMDLKLPTFPDISWLTKGSNMAKEIPTLR 1380

Query: 1381 DYLESCLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNW 1440
            D LE+CL  YL QTS+IM QQLAMEEAHIMLQK AKLEL KF YFIVP WV IFRRI NW
Sbjct: 1381 DNLENCLSCYLTQTSKIMEQQLAMEEAHIMLQKCAKLELHKFCYFIVPHWVKIFRRISNW 1440

Query: 1441 RLRYLAGRSSYVHIVDCYHSVSPTSGVKLERREPPSYRPDQPLLDELIEAACSSLSIDQR 1500
            RLRY +GRSSYV+IVDC H  S  S V+LE REPPSY P+QPLLDE+IE ACSSLSID+ 
Sbjct: 1441 RLRYFSGRSSYVNIVDCCHGASAVSSVRLESREPPSYTPNQPLLDEVIEVACSSLSIDEG 1473

Query: 1501 TQLPDAHQPLAAMTSNSRPPEVAAVAIDFSDNDRDSTREIGFVSSEC-VPNLGQRLSCTG 1560
              L +AHQPLAA+TSNSRP E+    I+F+D+  ++TR++GFVSS+  VPN G+ L+CTG
Sbjct: 1501 RPLSEAHQPLAAITSNSRPRELVVTTINFADDGSNNTRQVGFVSSDSIVPNSGRELNCTG 1473

Query: 1561 KELVASSTVYSEAVRLNELLDRCNELQDAIEKKLSIYF 1570
            KE+VA+ T YSEA RL +LLD+C++ QDAIEK LSIY+
Sbjct: 1561 KEVVANDTRYSEAARLKKLLDQCDKRQDAIEKVLSIYY 1473

BLAST of MC05g_new0080 vs. ExPASy TrEMBL
Match: A0A6J1IQW1 (SAC3 family protein B OS=Cucurbita maxima OX=3661 GN=LOC111479178 PE=4 SV=1)

HSP 1 Score: 2082 bits (5394), Expect = 0.0
Identity = 1113/1595 (69.78%), Postives = 1236/1595 (77.49%), Query Frame = 0

Query: 1    MSYQGFGKASGPSAPPKLQHSFGSSARPVSVSP--SPSTFPAFSDASPPRAPRVQSHPMA 60
            MSYQGFGKASGPSAPPKLQHSFG+SA P SVSP  + S   AF         RVQSH MA
Sbjct: 1    MSYQGFGKASGPSAPPKLQHSFGNSAIPDSVSPLRASSQHEAFGS-------RVQSHSMA 60

Query: 61   FQTTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSRGPILFPTRSV 120
            FQT G A SLHHQY                                              
Sbjct: 61   FQTPGTASSLHHQY---------------------------------------------- 120

Query: 121  EEQHSSFKAVDAQSHPSRGITSVASPQTSDHAVGEVAHLLTKRSRSPTYMPGDEKLSKIA 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  GHPVIRRPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGN 240
                  RP SSS  F+ QPK+RG+  NS +YQDQ  S+RS+D DTNI       QAPK  
Sbjct: 181  -----YRPVSSSPIFEEQPKVRGMLPNSQAYQDQALSERSHDHDTNI-------QAPKRT 240

Query: 241  RSPEQPSASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPK 300
             SPE+P  S+LR AQ+NL R S SPPR FS  N  E  GSM+N+ +ES  T+P  VLV K
Sbjct: 241  ESPEKPFVSNLRSAQTNLLRPSTSPPRPFSWSNTQEAAGSMRNINTESVVTEPTGVLVSK 300

Query: 301  RTRSPPLPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNG 360
            RT+SP L SSD V GGNS  T DD ERERLAKAKRL RFK ELVE A S LGS+D RDN 
Sbjct: 301  RTKSPTLSSSDQVSGGNSDPTPDDTERERLAKAKRLARFKVELVEVAHSKLGSVDARDNS 360

Query: 361  NKNEHSMADPNKYLSNQSLKLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERE 420
            N+NEHS  + +K +SNQSL+ S N+A GN MPDYEALESSSII+GLCPDMCPESERAERE
Sbjct: 361  NRNEHSTTERDKCMSNQSLESSTNLAQGNSMPDYEALESSSIIIGLCPDMCPESERAERE 420

Query: 421  RKGDLDHYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYD 480
            RKGDLDHYERLDGDRN +SKLLAVKKY RTAEREANLIRPMP+L KTI YLL LLSQPY 
Sbjct: 421  RKGDLDHYERLDGDRNQTSKLLAVKKYTRTAEREANLIRPMPVLLKTIKYLLELLSQPYG 480

Query: 481  EKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540
            EKFLG YNFLWDRMRAIRMDLRMQHLFNE+AITMLEQMIRLHIIAMHELCEFSKGEGFAE
Sbjct: 481  EKFLGIYNFLWDRMRAIRMDLRMQHLFNENAITMLEQMIRLHIIAMHELCEFSKGEGFAE 540

Query: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600
            GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL
Sbjct: 541  GFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAEL 600

Query: 601  SLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660
            SLDLAKMTPEMRQT EVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ
Sbjct: 601  SLDLAKMTPEMRQTAEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQ 660

Query: 661  ALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSD 720
            ALASLHSG+QNNQGLPI+HV KWIGMEEEDIEGLLEYHGF IKVFEEPYMVREGPFLNSD
Sbjct: 661  ALASLHSGVQNNQGLPISHVRKWIGMEEEDIEGLLEYHGFSIKVFEEPYMVREGPFLNSD 720

Query: 721  KDFATKCSKLVHMKRSRMIVNDVSPCSQNENLITGATKEIPLP----------------- 780
            KDFATKCSKLVHMKRS MIV DVSP S+NE LITGATK+ PL                  
Sbjct: 721  KDFATKCSKLVHMKRSGMIVTDVSPKSRNEYLITGATKQTPLTWKSKNEYLSTSAIKQIP 780

Query: 781  --RIRKESQTFSFEKISSPRTVSAKKE--SSIRVTNEEMTEFDDQPTPVDHKQVQPMIET 840
                +K+   FSFEKI+SPR VS +KE  SS+   +E+M EFDD   P+D KQVQPMIET
Sbjct: 781  SKMTKKQLPIFSFEKITSPRPVSPEKEKESSVNEIDEDMVEFDDPLIPIDPKQVQPMIET 840

Query: 841  PKVSQLHEYNHEVDGALVQQSGLRSCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVS 900
             +V QLHEYNH V+GAL+ QSG RSCEPL+TE+KFVGNQ YDG+F TS ++NISTG+G+S
Sbjct: 841  SEVGQLHEYNHGVNGALLLQSGPRSCEPLRTEVKFVGNQGYDGVFVTSPVRNISTGMGMS 900

Query: 901  LPLVTEASLQNISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQDIVMESCQDEE 960
            LPLV++AS Q ISVCGYND+A+  VEPQ +VNN+MED+E LYA+ ENK DIV E C DEE
Sbjct: 901  LPLVSDASPQKISVCGYNDNAIGSVEPQSIVNNMMEDEEILYATQENKHDIVTEGCPDEE 960

Query: 961  IANARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLNNHKIRSIGVFDID 1020
            IA+ARLKLILRLWRRRA+++KQLREQR+LAAKAAF TLSVGPPVQL NHKIRS+GVFDID
Sbjct: 961  IADARLKLILRLWRRRAVRRKQLREQRLLAAKAAFDTLSVGPPVQLKNHKIRSVGVFDID 1020

Query: 1021 HTLRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDNVGDLDQMGQDS 1080
            H LRERWK+Q+LS S+VNVS+VVAS++  +NV+ KCICWKLV+CSQMD+ GDL Q   DS
Sbjct: 1021 HILRERWKRQKLSCSVVNVSEVVASSLGGKNVNGKCICWKLVVCSQMDSAGDLVQGTGDS 1080

Query: 1081 RFAAGYWLLSKLMPSKADDLVLSSSFLSIWRSWISSEIGANLSCFLSIVKDTEFDNLTET 1140
              +AG WLLSKLMPS+A+DLV SSSFLSIW+SW+S + G + SCFLS+VK   FDNL ET
Sbjct: 1081 HISAGSWLLSKLMPSEANDLVFSSSFLSIWKSWLSGKTGVDSSCFLSVVKHANFDNLPET 1140

Query: 1141 VHGASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLILSDSHDEVSASSMLVNKLG 1200
            V GASAILFVAT+S P +LQRVQLHKLL+SIPSGSCLPLLILSD HDE SASSMLVNKLG
Sbjct: 1141 VRGASAILFVATQSTPLDLQRVQLHKLLLSIPSGSCLPLLILSDLHDEASASSMLVNKLG 1200

Query: 1201 LYDIDKSRIHSFQVVSLLENPHLRHLGFFSDEKLKEGLKWLANESPPQPVLHRVKVLNLI 1260
            LYDIDKSRI SFQV+SLL+NPHLRH GF SDEKLKEGLKWLANESP QPVLH VKVL+LI
Sbjct: 1201 LYDIDKSRICSFQVISLLDNPHLRHSGFLSDEKLKEGLKWLANESPSQPVLHSVKVLDLI 1260

Query: 1261 ITHLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDVTAAAKENPSNWPCPEIALLES 1320
            ITHL+ S+EMLDSM E DVSPNQCISAFNLALDQSL D+TAA K NPSNWPCPEIA L S
Sbjct: 1261 ITHLDLSMEMLDSMNEKDVSPNQCISAFNLALDQSLADITAAVKANPSNWPCPEIASLVS 1320

Query: 1321 TDEHTITTHALPPVGWSSVENVEPLRQALMDLKLPSFPDMSWLSKGSNMVNEIPNHRDYL 1380
             +EH I T+ALPPVGWSS ENVEPL++ALMDLKLP+FPD+SWL+KGSNM  EIP  RD L
Sbjct: 1321 CNEHGIITNALPPVGWSSAENVEPLKKALMDLKLPTFPDISWLTKGSNMAKEIPTLRDNL 1380

Query: 1381 ESCLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLELQKFKYFIVPDWVTIFRRIFNWRLR 1440
            E+CL  YL QTS+IM QQLAMEEAHIMLQK AKLEL KF YFIVP WV IFRRI NWRLR
Sbjct: 1381 ENCLSCYLTQTSKIMEQQLAMEEAHIMLQKCAKLELHKFCYFIVPHWVKIFRRISNWRLR 1440

Query: 1441 YLAGRSSYVHIVDCYHSVSPTSGVKLERREPPSYRPDQPLLDELIEAACSSLSIDQRTQL 1500
            Y AGRSSYV+IVDC H  S  S V+LE REPPSY P+QPLLDE+IE ACSSLSID+   L
Sbjct: 1441 YFAGRSSYVNIVDCCHGASVVSSVRLESREPPSYTPNQPLLDEVIEVACSSLSIDEGRPL 1470

Query: 1501 PDAHQPLAAMTSNSRPPEVAAVAIDFSDNDRDSTREIGFVSSECV-PNLGQRLSCTGKEL 1560
             +AHQPLAA+TSNSRP E+    I+F+D+  ++TR++GFVSS+ V PN  + L+CTGKEL
Sbjct: 1501 SEAHQPLAAITSNSRPCELIVTTINFADDGSNNTRQVGFVSSDSVVPNSDRELNCTGKEL 1470

Query: 1561 VASSTVYSEAVRLNELLDRCNELQDAIEKKLSIYF 1570
            VAS T YSEA +L +LLD+C++ QDAIEK LSIY+
Sbjct: 1561 VASDTRYSEAAKLKKLLDQCDKRQDAIEKILSIYY 1470

BLAST of MC05g_new0080 vs. ExPASy TrEMBL
Match: A0A0A0L8V9 (PCI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G150220 PE=4 SV=1)

HSP 1 Score: 1918 bits (4968), Expect = 0.0
Identity = 1014/1421 (71.36%), Postives = 1145/1421 (80.58%), Query Frame = 0

Query: 185  RPASSSHTFDNQPKMRGIHVNSHSYQDQYPSKRSYDQDTNILTGFGNVQAPKGNRSPEQP 244
            R  S S T ++QPK++GI  N  +YQ +  SK+SYD ++NILT FGNVQAPK  +SPE+ 
Sbjct: 39   RTVSPSPTVEDQPKVQGILPNVQAYQVRSTSKQSYDLESNILTEFGNVQAPKRTKSPER- 98

Query: 245  SASDLRYAQSNLQRSSISPPRSFSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRTRSPP 304
                LR AQ+NL R S SPPRSFSR NAHEV  SM+   +ES A    SVLVPKRTRSP 
Sbjct: 99   ----LRSAQTNLPRPSTSPPRSFSRANAHEVMSSMRTTDAESVA----SVLVPKRTRSPT 158

Query: 305  LPSSDLVFGGNSHSTCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNKNEHS 364
            LPSSD V G N + T DD ERERLAKAKRL RFK EL +  ++ +G +D  DN N+NE S
Sbjct: 159  LPSSDQVSGPNFYPTHDDTERERLAKAKRLARFKVELDDVTQNKMGGVDVMDNTNRNECS 218

Query: 365  MADPNKYLSNQSLKLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERERKGDLD 424
              + +K++S+QSL  S N+A GN +PD + +ESSSII+GLCPDMCPESER ERERKGDLD
Sbjct: 219  TTERDKFMSSQSLDSSRNLAHGNSIPDND-VESSSIIIGLCPDMCPESERGERERKGDLD 278

Query: 425  HYERLDGDRNHSSKLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKFLGT 484
            HYERLDGDRN +SKLLAVKKY RTAEREA LIRPMP+L KTIDYLL+LLSQPYDEKFLG 
Sbjct: 279  HYERLDGDRNQTSKLLAVKKYTRTAEREAILIRPMPVLLKTIDYLLDLLSQPYDEKFLGI 338

Query: 485  YNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHL 544
            YNFLWDRMRAIRMDLRMQHLFNE+AITMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHL
Sbjct: 339  YNFLWDRMRAIRMDLRMQHLFNENAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHL 398

Query: 545  NIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAK 604
            NIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAK
Sbjct: 399  NIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAK 458

Query: 605  MTPEMRQTPEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALASLH 664
            MTPEMRQT EVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALASLH
Sbjct: 459  MTPEMRQTAEVKFARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALASLH 518

Query: 665  SGLQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDFATK 724
            SG+QNNQGLPIAHVCKWIGMEEEDIEGLLEYHGF IKVFEEPYMVREGPFLN DKDF TK
Sbjct: 519  SGVQNNQGLPIAHVCKWIGMEEEDIEGLLEYHGFSIKVFEEPYMVREGPFLNGDKDFPTK 578

Query: 725  CSKLVHMKRSRMIVNDVSP-----C--------------SQNENLITGATKEIPLPRIRK 784
            CSKLVHMKRSRMIVNDV P     C              S+NE+LI  ATK+IPL R +K
Sbjct: 579  CSKLVHMKRSRMIVNDVLPKSKTECLINGATKMIPLTRKSKNEHLIPDATKQIPLTRTKK 638

Query: 785  ESQTFSFEKISSPRTVSAKKESSIRVTNEEMTEFDDQPTPVDHKQVQPMIETPKVSQLHE 844
            E +TF+FEKISSPR +S KKESSI   +EEMTEFDDQ  PVDHKQVQP IET +V QLHE
Sbjct: 639  EPKTFAFEKISSPRPISTKKESSIHEIDEEMTEFDDQLIPVDHKQVQPKIETLEVRQLHE 698

Query: 845  --YNHEVDGALVQQSGLRSCEPLKTEIKFVGNQNYDGLFTTSSMKNISTGIGVSLPLVTE 904
              YNHE +G L+Q S  RSCEPL+TE++FVGNQ YDGLF TS + + S  +G+ LPLV++
Sbjct: 699  NKYNHEENGGLLQSSP-RSCEPLRTEVQFVGNQGYDGLFMTSPVGSNSARMGLPLPLVSD 758

Query: 905  ASLQ-------------NISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQDIVM 964
            A LQ              IS  GYN++ +  VEPQI+VNNVMED+E L A+ E+K DI+ 
Sbjct: 759  APLQMISASGYKQAPLQKISAFGYNNNTIRSVEPQIIVNNVMEDEEILNATQESKIDIIT 818

Query: 965  ESCQDEEIANARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLNNHKIRS 1024
            +SC DEEIANARLKLILRLW+RRALK+KQLREQR+LAAKAAF  LSVGPP+QLN+HKIRS
Sbjct: 819  DSCPDEEIANARLKLILRLWKRRALKRKQLREQRLLAAKAAFDILSVGPPIQLNSHKIRS 878

Query: 1025 IGVFDIDHTLRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDNVGDL 1084
             G+FDIDH + ERWK+Q++S S+VNVS+VVAS +SRRNVD KC+CWKLV+CSQ       
Sbjct: 879  NGIFDIDHIVSERWKRQKMSCSVVNVSEVVASILSRRNVDGKCVCWKLVVCSQGT----- 938

Query: 1085 DQMGQDSRFAAGYWLLSKLMPSKADDLVLSSSFLSIWRSWISSEIGANLSCFLSIVKDTE 1144
                +DS F AG WLLSKLMPS+A+DLV SSSFLSIW+SW+S   G +LSCFLSIV+   
Sbjct: 939  ----RDSHFVAGSWLLSKLMPSEANDLVFSSSFLSIWKSWLSGITGLDLSCFLSIVRHAN 998

Query: 1145 FDNLTETVHGASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLILSDSHDEVSASS 1204
            FDNL ETVHGA AILFVATESIP +LQRVQLH+L+ SI SGSCLPLLILSD  DE+SAS 
Sbjct: 999  FDNLPETVHGAGAILFVATESIPLDLQRVQLHELVASISSGSCLPLLILSDFDDEISAS- 1058

Query: 1205 MLVNKLGLYDIDKSRIHSFQVVSLLENPHLRHLGFFSDEKLKEGLKWLANESPPQPVLHR 1264
             L NKL LY+IDKSRIHSFQ+VSLL+NPHLRHLGFFSDEKLKEGLKWLANESP QPVLHR
Sbjct: 1059 -LANKLDLYNIDKSRIHSFQIVSLLDNPHLRHLGFFSDEKLKEGLKWLANESPTQPVLHR 1118

Query: 1265 VKVLNLIITHLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDVTAAAKENPSNWPCP 1324
            V+VL+LII+HL+SS+++LDSM E DVSPN CISAFNLALDQS+ D+TAA K NPSNWPCP
Sbjct: 1119 VRVLDLIISHLDSSMKVLDSMNEKDVSPNHCISAFNLALDQSVADITAAVKANPSNWPCP 1178

Query: 1325 EIALLESTDEHTITTHALPPVGWSSVENVEPLRQALMDLKLPSFPDMSWLSKGSNMVNEI 1384
            EIALLES  E    T ALPPVGWS VENVEPL+QALMDLKLP+F D+SWL+K SN + EI
Sbjct: 1179 EIALLESCSEPAFVTDALPPVGWSFVENVEPLKQALMDLKLPTFLDISWLTKVSNTIKEI 1238

Query: 1385 PNHRDYLESCLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLELQKFKYFIVPDWVTIFRR 1444
            P  RD LESCL  YL +TSEIM QQLA+EEAHIMLQK AKLE   F Y IVP WVTIFRR
Sbjct: 1239 PTVRDNLESCLRCYLTKTSEIMVQQLALEEAHIMLQKCAKLEHHNFNYSIVPRWVTIFRR 1298

Query: 1445 IFNWRLRYLAGRSSYVHIVDCYHSVSPTSGVKLERREPPSYRPDQPLLDELIEAACSSLS 1504
            IFNWRLR    RSSY HIV+C H  S +S  +LE REPP YRP+QPLLDE+IE A SSL 
Sbjct: 1299 IFNWRLRCFPSRSSYAHIVNCCHGASVSSSTRLESREPPLYRPNQPLLDEVIEVAFSSLL 1358

Query: 1505 IDQRTQLPDAHQPLAAMTSNSRPPEVAAVAIDFSDNDRDSTREIGFVSSECVPNLGQRLS 1564
            I+Q    P+AHQP    TSN RP EV    I+F +++   T+++GF S   V N  + L+
Sbjct: 1359 INQERDFPEAHQPPVTTTSNGRPHEVVVATINFPNDNGYRTQQMGFGSPNSVANSDRELN 1418

Query: 1565 CTGKELVASSTVYSEAVRLNELLDRCNELQDAIEKKLSIYF 1570
            C GKE+V S   YSEA RL ELLD+CN+ Q+AIEK LS+YF
Sbjct: 1419 CAGKEVVVSDRGYSEAERLKELLDQCNKRQNAIEKMLSVYF 1437

BLAST of MC05g_new0080 vs. TAIR 10
Match: AT3G06290.1 (SAC3/GANP/Nin1/mts3/eIF-3 p25 family )

HSP 1 Score: 1059.3 bits (2738), Expect = 3.0e-309
Identity = 718/1684 (42.64%), Postives = 968/1684 (57.48%), Query Frame = 0

Query: 7    GKASGPS---APP-----KLQHSFGSSARPVSVS--------PSPSTF--PAFSDASPPR 66
            G  SGPS   APP       Q S G   RP  V         PSPS F  P+ S   P +
Sbjct: 72   GIRSGPSIQRAPPLSASQNPQLSIGKPYRPGGVQSVPPINRIPSPSAFQNPSPSSGQPYQ 131

Query: 67   APRVQSHPMAFQTTGAAPSLHHQYDRGPRATVRMGSPSLAFESTRSAATESYSYPSVGSR 126
               +Q  P  F   G A         GP A            S  S +   Y +P  G +
Sbjct: 132  PGGIQRIPEPF--NGIA--------WGPEA------------SRTSPSVRPYQFP--GVQ 191

Query: 127  GPILFPTRSVE---------EQHSSFKAVDAQSH--PSRGITSVASPQTSDHAVGEVAHL 186
             P L P    +          +HS   +  A SH     G  +V   ++ D      + +
Sbjct: 192  RPNLNPQYGHDGSRNFLKDHGEHSRATSPPATSHILSRMGTDAVEIGRSQDSKRKSRSDI 251

Query: 187  L-------TKRSRSPTYMPGDEKLSKIAGHPVI--RRPASSSHTFDNQPKMRGIHVNSHS 246
            L       ++R++SP  + G E  + + G   +  R    S  + +N P     + N   
Sbjct: 252  LPDQNMGFSRRNQSP--VSGFENGNLVDGFQPLSSRTWMRSPSSAENNPVRSRSNPNQLI 311

Query: 247  YQDQ-----YPSKRSYDQDTNILTGFGNVQAPKGNRSPEQPSASDLRYAQSNLQRSSISP 306
            +Q+Q     +P      +         +  AP      ++P   D   +Q + QR S SP
Sbjct: 312  HQEQTGNSSFPYAHEVAEIQEATRRKSSAVAPS-----DKPLGDDPILSQHDSQRFSTSP 371

Query: 307  PRS------FSRLNAHEVGGSMKNVYSESEATKPNSVLVPKRTRSPPLPSSDLVFGGNSH 366
            P S       SR +  +  G   +V S + A K NS    KRTRSPP+   +     NS 
Sbjct: 372  PTSGTKSYTLSRSSDSQFPGQPSSVNSFNNARKTNSSPATKRTRSPPVYPIEEDIPRNSF 431

Query: 367  STCDDAERERLAKAKRLVRFKDELVEAARSNLGSMDERDNGNKNEHSMADPNKYLSNQSL 426
             + D  E E  A+AKRL RFK EL   A   +     +   NK    +   NK   N SL
Sbjct: 432  PSQDCTEGEEQARAKRLARFKGELEPIADRPVDIQLTKSPVNKTMKPL--DNKQTFN-SL 491

Query: 427  KLSGNVADGN-MPDYEALESSSIIVGLCPDMCPESERAERERKGDLDHYERLDGDRNHSS 486
            + S +   G+ +PDYE  E  S+I+G+CPDMCPESER ERERKGDLDHYER+DGDRN +S
Sbjct: 492  ESSRDALKGDALPDYENSEQPSLIIGVCPDMCPESERGERERKGDLDHYERVDGDRNQTS 551

Query: 487  KLLAVKKYNRTAEREANLIRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRM 546
            K LAVKKY RTAEREA LIRPMPIL+ T++YLL+LL +PY+E FLG YNFLWDRMRAIRM
Sbjct: 552  KSLAVKKYTRTAEREAILIRPMPILQNTMEYLLSLLDRPYNENFLGMYNFLWDRMRAIRM 611

Query: 547  DLRMQHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELF 606
            DLRMQH+FN++AIT+LEQMIRLHIIAMHELCE++KGEGF+EGFDAHLNIEQMNKTSVELF
Sbjct: 612  DLRMQHIFNQEAITLLEQMIRLHIIAMHELCEYTKGEGFSEGFDAHLNIEQMNKTSVELF 671

Query: 607  QMYDDHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKF 666
            QMYDDHRK+GI VP+EKEFRGYYALLKLDKHPGYKVEP+ELSLDLA MTPE+RQT EV F
Sbjct: 672  QMYDDHRKKGITVPTEKEFRGYYALLKLDKHPGYKVEPSELSLDLANMTPEIRQTSEVLF 731

Query: 667  ARDVARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALASLHSGLQNNQGLPIAH 726
            AR+VARACRT NFIAFFRL RKASYLQACLMHAHF+KLRTQALASLHSGLQ NQGLP++ 
Sbjct: 732  ARNVARACRTGNFIAFFRLARKASYLQACLMHAHFSKLRTQALASLHSGLQINQGLPVSD 791

Query: 727  VCKWIGMEEEDIEGLLEYHGFLIKVFEEPYMVREGPFLNSDKDFATKCSKLVHMKRSRMI 786
            +  WIGMEEEDIE LLEYHGF IKVFEEPYMV+   FL++DKD+ TKCSKLVHMK+SR I
Sbjct: 792  MSNWIGMEEEDIEALLEYHGFSIKVFEEPYMVKNDLFLHADKDYKTKCSKLVHMKKSRTI 851

Query: 787  VNDVSPCSQNENLITGATKEIPLPRIRKES-----QTFSFEKISSPRTVSAKKESSIRVT 846
            V DVS  +  E++ T      PLP +  E+     Q  +  K   P   S KK++S+R+ 
Sbjct: 852  VEDVSAPTVVEDVST----PFPLPSLITEATIGNQQCITAHKHEMPPARSLKKQTSMRLF 911

Query: 847  NEEMTEF-------DDQP--------------TPVDHKQVQPMIE--------------- 906
            ++E+ +        +D+P               PV H++ Q  +                
Sbjct: 912  DKEVADSKTSLLAEEDKPMGTFVMNPPGPFVINPVVHQEKQNDLTSAGGFHSPVKLYSPF 971

Query: 907  -TPKVSQLHEYNHEV---DGALVQQSGLRSCEPLKTEIKF-----VGNQNYDGLFTTSSM 966
             +PK  Q    N E    D  +    G         EIKF     V   +  G     S 
Sbjct: 972  GSPKFPQTKSSNLEKQPNDDRIGMSPG---------EIKFSIIGDVYTNHVPGPALQQSP 1031

Query: 967  KNISTGIGVSLPLVTEASLQNISVCGYNDSAMERVEPQILVNNVMEDKETLYASVENKQD 1026
            K++   I   +P+ T A     SV   N  A+E   P+  +   +E +       +  +D
Sbjct: 1032 KSMPMEI---MPVTTIAECPT-SV--ENKYALEESVPEAAMICTLEKEFNDIDEEDEDED 1091

Query: 1027 IVMESCQDEEIANARLKLILRLWRRRALKKKQLREQRVLAAKAAFSTLSVGPPVQLN-NH 1086
             V+ +  DEE+A A+LKLI+RLW+R + ++ +LRE+R LAA AA ++LS+G P++ +   
Sbjct: 1092 GVILNQYDEEVAKAKLKLIIRLWKRWSSRQSELRERRQLAAAAALNSLSLGTPIRFSKTD 1151

Query: 1087 KIRSIGVFDIDHTLRERWKKQELSWSIVNVSDVVASTMSRRNVDVKCICWKLVICSQMDN 1146
            + R+ G F+ID  +R R++++E SWS +N+SDV+A  +  RN + KCI WK+V+C+Q  +
Sbjct: 1152 QSRACGEFNIDQAMRRRFEEREKSWSRLNISDVIADILVGRNPESKCISWKVVLCTQTKS 1211

Query: 1147 VGDLDQMGQDSRFAAGYWLLSKLMPS------KADDLVLSSSFLSIWRSWISSEIGANLS 1206
            V       Q +  AA  WL SKLMP         D+L+ S+  +S+W  W+++    + +
Sbjct: 1212 VNSSSSASQVTHSAASRWLSSKLMPHAEHSSLNDDNLLFSAPGVSVWNKWVANGSDIDFT 1271

Query: 1207 CFLSIVKDTEFDN-LTETVHGASAILFVATESIPWELQRVQLHKLLMSIPSGSCLPLLIL 1266
            C LS+ +D E +N + ET  GASA+LF+A+  +P  LQR QL+ +L S+P+GS LPLL++
Sbjct: 1272 CCLSVARDVEAENDMCETTCGASAVLFLASGGLPLNLQREQLNLILESVPNGSVLPLLVV 1331

Query: 1267 --SDSHDEVSASSMLVNKLGLYDIDKSRIHSFQVVSLLENPHL-RHLGFFSDEKLKEGLK 1326
              S + + +   + +V+ LGL+DIDKS+I SF +VS+       + + FF+D +L++G K
Sbjct: 1332 ISSCNGEHMEPDTDIVSGLGLHDIDKSKIASFSIVSIANKSQKGQEVHFFNDSRLRDGFK 1391

Query: 1327 WLANESPPQPVLHRVKVLNLIITHLNSSIEMLDSMKENDVSPNQCISAFNLALDQSLTDV 1386
            WLA+ SP QP LH VK+  L +TH + S+E+L  M + +V PN CISAFN AL+ S  ++
Sbjct: 1392 WLASNSPLQPNLHHVKLRELFLTHFSFSLELLKQMPDQEVGPNICISAFNDALETSRRNI 1451

Query: 1387 TAAAKENPSNWPCPEIALLE-STDEHTITTHALPPVGWSSVENVEPLRQALMDLKLPSF- 1446
            T+AA+ NP  WPCPE  LLE +  E  +    LP + WSS ENVE L   L + KLP F 
Sbjct: 1452 TSAAEANPIGWPCPETMLLEDNRKECLMVKRYLPNLDWSSAENVELLSSVLENCKLPDFE 1511

Query: 1447 PDMSWLSKGSNMVNEIPNHRDYLESCLISYLNQTSEIMGQQLAMEEAHIMLQKSAKLEL- 1506
             D++WL+ G     EI NH   LE CLI YL Q S +MG  LA +E  +ML+++ +LEL 
Sbjct: 1512 DDLTWLTVGCASGAEIENHTQRLEGCLIEYLTQRSNLMGVSLATKETGVMLERNTRLELH 1571

Query: 1507 QKFKYFIVPDWVTIFRRIFNWRLRYL---AGRSSYVHIVDCYHSVSPTSGVKL-ERREPP 1566
               +Y I P W+ IF+RIFNWR+  L   +  S+YV   D   S S  +   L E    P
Sbjct: 1572 NSSRYHITPRWIGIFQRIFNWRIMGLFDASSSSAYVLKSDLNMSTSSYADKFLAEDASYP 1631

Query: 1567 SYRPDQPLLDELIEAACSSLSIDQRTQLPDAHQPLAAMTSNSRPPEVAAVAIDFSDNDRD 1571
            S  P+ PLL E+IE +CS L    ++  P   +    + +     +   +     + +R+
Sbjct: 1632 SCPPNLPLLHEMIEISCSPL----KSPPPYDDKAQRVVETGMLIDDHRDIEESMLEKNRE 1691

BLAST of MC05g_new0080 vs. TAIR 10
Match: AT3G54380.1 (SAC3/GANP/Nin1/mts3/eIF-3 p25 family )

HSP 1 Score: 179.9 bits (455), Expect = 1.6e-44
Identity = 113/298 (37.92%), Postives = 174/298 (58.39%), Query Frame = 0

Query: 398 SIIVGLCPDMCPESERAERERKGDLDHYERLDGDRNHSSKLLAVKKYNRT---AEREANL 457
           S+IVG C  MCPE ER  RER  DL  +ERL G+ + SS  +AVKK+ RT   A+ +A+ 
Sbjct: 68  SLIVGTCSSMCPERERVTRERLRDLAVFERLYGNPSKSSTEIAVKKFCRTLSAADVQASD 127

Query: 458 IRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQ 517
           +RP+P+L +T+ YLL+LL    +  F   ++F++DR R+IR DL +Q+L NE  I + E+
Sbjct: 128 VRPLPVLEETLRYLLSLLDSK-EHPFEVVHDFIFDRTRSIRQDLSIQNLANERVIYLYEE 187

Query: 518 MIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKE 577
           M++ H+I+ HE  +   G   +     HLN+EQ+ KT   L+ +YD +RK   I  +E E
Sbjct: 188 MVKFHVIS-HERLQSCSGTSISSMH--HLNMEQLAKTLTSLYNIYDANRKPDYIYENEAE 247

Query: 578 FRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFF- 637
           FR  Y LL L+   G   EP  LSL   K+T  + ++ E+ F R++ R  R  N+  F  
Sbjct: 248 FRSLYVLLHLNPSSGVMGEP--LSLWFRKLTFALVKSKEICFVRNLLRLYRMGNYKNFLS 307

Query: 638 RLVRKASYLQACLMHAHFAKLRTQALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGL 692
           R   +A+YLQ C+   H  ++R  A+  +++     Q  P+  + + + M+E D+E L
Sbjct: 308 RTASEATYLQYCISEHHIREMRLVAVQYINNVCYKLQPYPLLRLSQNLKMKELDVESL 359

BLAST of MC05g_new0080 vs. TAIR 10
Match: AT3G54380.2 (SAC3/GANP/Nin1/mts3/eIF-3 p25 family )

HSP 1 Score: 171.8 bits (434), Expect = 4.4e-42
Identity = 109/291 (37.46%), Postives = 169/291 (58.08%), Query Frame = 0

Query: 398 SIIVGLCPDMCPESERAERERKGDLDHYERLDGDRNHSSKLLAVKKYNRT---AEREANL 457
           S+IVG C  MCPE ER  RER  DL  +ERL G+ + SS  +AVKK+ RT   A+ +A+ 
Sbjct: 68  SLIVGTCSSMCPERERVTRERLRDLAVFERLYGNPSKSSTEIAVKKFCRTLSAADVQASD 127

Query: 458 IRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQ 517
           +RP+P+L +T+ YLL+LL    +  F   ++F++DR R+IR DL +Q+L NE  I + E+
Sbjct: 128 VRPLPVLEETLRYLLSLLDSK-EHPFEVVHDFIFDRTRSIRQDLSIQNLANERVIYLYEE 187

Query: 518 MIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKE 577
           M++ H+I+ HE  +   G   +     HLN+EQ+ KT   L+ +YD +RK   I  +E E
Sbjct: 188 MVKFHVIS-HERLQSCSGTSISSMH--HLNMEQLAKTLTSLYNIYDANRKPDYIYENEAE 247

Query: 578 FRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFF- 637
           FR  Y LL L+   G   EP  LSL   K+T  + ++ E+ F R++ R  R  N+  F  
Sbjct: 248 FRSLYVLLHLNPSSGVMGEP--LSLWFRKLTFALVKSKEICFVRNLLRLYRMGNYKNFLS 307

Query: 638 RLVRKASYLQACLMHAHFAKLRTQALASLHSGLQNNQGLPIAHVCKWIGME 685
           R   +A+YLQ C+   H  ++R  A+  +++     Q  P+  + + + M+
Sbjct: 308 RTASEATYLQYCISEHHIREMRLVAVQYINNVCYKLQPYPLLRLSQNLKMK 352

BLAST of MC05g_new0080 vs. TAIR 10
Match: AT3G54380.3 (SAC3/GANP/Nin1/mts3/eIF-3 p25 family )

HSP 1 Score: 159.1 bits (401), Expect = 3.0e-38
Identity = 104/284 (36.62%), Postives = 164/284 (57.75%), Query Frame = 0

Query: 412 ERAERERKGDLDHYERLDGDRNHSSKLLAVKKYNRT---AEREANLIRPMPILRKTIDYL 471
           ER  RER  DL  +ERL G+ + SS  +AVKK+ RT   A+ +A+ +RP+P+L +T+ YL
Sbjct: 10  ERVTRERLRDLAVFERLYGNPSKSSTEIAVKKFCRTLSAADVQASDVRPLPVLEETLRYL 69

Query: 472 LNLLSQPYDEKFLGTYNFLWDRMRAIRMDLRMQHLFNEDAITMLEQMIRLHIIAMHELCE 531
           L+LL    +  F   ++F++DR R+IR DL +Q+L NE  I + E+M++ H+I+ HE  +
Sbjct: 70  LSLLDSK-EHPFEVVHDFIFDRTRSIRQDLSIQNLANERVIYLYEEMVKFHVIS-HERLQ 129

Query: 532 FSKGEGFAEGFDAHLNIEQMNKTSVELFQMYDDHRKRGIIVPSEKEFRGYYALLKLDKHP 591
              G   +     HLN+EQ+ KT   L+ +YD +RK   I  +E EFR  Y LL L+   
Sbjct: 130 SCSGTSISSMH--HLNMEQLAKTLTSLYNIYDANRKPDYIYENEAEFRSLYVLLHLNPSS 189

Query: 592 GYKVEPAELSLDLAKMTPEMRQTPEVKFARDVARACRTSNFIAFF-RLVRKASYLQACLM 651
           G   EP  LSL   K+T  + ++ E+ F R++ R  R  N+  F  R   +A+YLQ C+ 
Sbjct: 190 GVMGEP--LSLWFRKLTFALVKSKEICFVRNLLRLYRMGNYKNFLSRTASEATYLQYCIS 249

Query: 652 HAHFAKLRTQALASLHSGLQNNQGLPIAHVCKWIGMEEEDIEGL 692
             H  ++R  A+  +++     Q  P+  + + + M+E D+E L
Sbjct: 250 EHHIREMRLVAVQYINNVCYKLQPYPLLRLSQNLKMKELDVESL 287

BLAST of MC05g_new0080 vs. TAIR 10
Match: AT2G39340.1 (SAC3/GANP/Nin1/mts3/eIF-3 p25 family )

HSP 1 Score: 48.9 bits (115), Expect = 4.3e-05
Identity = 54/250 (21.60%), Postives = 109/250 (43.60%), Query Frame = 0

Query: 442 KKYNR-TAEREANLIRPMPILRKTIDYLLNLLSQPYDEKFLGTYNFLWDRMRAIRMDLRM 501
           K+Y R T+  +   +RP  +L K +     ++ Q   + +L    F  D++++IR DL +
Sbjct: 730 KRYLRLTSAPDPATVRPEDVLEKAL-----IMVQDSQKNYL----FKCDQLKSIRQDLTV 789

Query: 502 QHLFNEDAITMLEQMIRLHIIAMHELCEFSKGEGFAEGFDAHLNIEQMNKTSVELFQMYD 561
           Q + N     + E   RL + A                     ++ + N+   +L  +Y 
Sbjct: 790 QRIHNHLTAKVYETHARLALEAG--------------------DLPEYNQCLSQLKTLYA 849

Query: 562 DHRKRGIIVPSEKEFRGYYALLKLDKHPGYKVEPAELSLDLAKMTPEMRQTPEVKFARDV 621
           +      +     EF   Y+LL +  H        EL   +++++ E ++   V+ A  V
Sbjct: 850 EG-----VEGCSLEFAA-YSLLYITLHSN---NNRELLSSMSRLSEEDKKDEAVRHALSV 909

Query: 622 ARACRTSNFIAFFRLVRKASYLQACLMHAHFAKLRTQALASLHSGLQNNQGLPIAHVCKW 681
             A  + N++ FFRL + A  + +CLM  +  K+R +A+  +    +    +P++++ + 
Sbjct: 910 RAAVTSGNYVMFFRLYKTAPNMNSCLMDLYVEKMRYKAVNFMSRSCRPT--IPVSYIVQV 939

Query: 682 IGMEEEDIEG 691
           +G      EG
Sbjct: 970 LGFTGAASEG 939