MC05g_new0047 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g_new0047
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncyclic nucleotide-gated ion channel 1-like
LocationMC05: 3187365 .. 3190345 (+)
RNA-Seq ExpressionMC05g_new0047
SyntenyMC05g_new0047
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCAGTTTTATGCTTCAAGCATGAATATGATCAGCCGAGGAAGAAACTGGAACTTTCGAAGAAAATTCTTGATCCACAGAAGCCATTTCTTCAGCAATGGAACAAGATATTTGTGCTCTCCTGTGTCCTAGCTGTAGCTGTGGACCCTCTCTTTTTTTACATCCCAGTGATTGATAGGCAACACCAATGCCTAACCATGGACCGGCTCCTTATGATCATTGCTTGCATTCTTCGTTCATTCATCGACATATTCTATCTACTTCACATTATCTTTGAATTCCGAACGGGCTTTCTACCTCCTTCTTTGCCAGTATTTGGAAGTGGCGAACTAGTTGAAGATCCAGCAGCTATAGCAAAGAGATACCTGTCCTCAAACTTCATAATTGATATCTTATCCATTATCCCTCTGCCTCAGGTATAATTTTAGTATAGTTTTAAGATCCTAGCAGCATCTGGTCTTTGCGTCAAGAGCATAACATTTGGCTATTCTCCTCATTTGGAATGCTAAATATTGGATGATAAGCTCTTTAAAACTCAGTCCACAATCTTCTCTTTCTCGATATTGTATTATCATTTTGTGTTTGTTCTGTTCTGTACGCTGTTTGAGAGTGGATTATTGTCGGAATCATTTGAGTTGATTCTTGTGAATCCTTTGTTTTAAGTAGAATTGACTGCCTCACAGAAGTGAACTTACTATTTACTTTTCATGTCATTCAGCTTTTGGTATTAGTTATCATACCTGCAGCGAAAGGCCCGATTGCATTGAGGACAAAGGACTTCGTGAAGGTTGCGGTTCTTTTGCAGTACATTCCAAGGCTTCTCCGAATTTATCTGCTATACAGAGAAGTAACCAGAACTTCTGGCATACTGACAGAAAGGGCCTGGAGTGGAGCTGCCTTCAATCTTCTTATTTATATGCTAGCTAGTCATGTATGTTGTGTTCATTTGATTTTCTAGCAATACAAAAATCAGCAGTTTTTTACATTTTGTTTAAAAGGGAGTTCTCATATGAGCCAGTGAATGTGATGAATTAGGTAGTTGGTGCCATTTGGTATTTGTTTTCAGTAGAACAAGAAGAAAGATGCTGGCTTGAGGCATGCAGAACACGTAACTGTAATAGCAAGGATCTGTGCTGTGGAGATCCCAGCAGAGCGGCATACATTATAAAGCAGTATTGCTCTCATAAAAAATCTGAAGATGACAAGACCTTCAACTTTGGAATGTATCTAGAAGCCCTTAAGTTTCATTTAACCGATCAGACGACGAATTTTCGCGACAAATTCTTTTATTGCTTCTGGTGGGCTCTGCGAAATGTCGGGTCTGTACAAGTTGCCCCTGTGTTTGTTCACTACTCTAGTGTGGAATGAGATATGAGATATGTGAATCTATTGTTATAACTTCCTATCTCTATCATCCATGTAGCAGATTTATTACAGTTTTTTAAGTTCATTGCTTTGGAACAACATACACCCTAAAAGAACTTGCCTGTCTTTTTGCAGCTCTTGTGGACAGAACCTTCAAATAAGCAATTACTTGGGGGAGGTTCTTTTTGCTGTCTTCATTGCCATCCTTGGTCTGCTCTTGTTTGCGTTACTCATTAGCAATATTCAGGTAATACATGACACTCATTCGTCTGTTTGTAGTAACTTCTCAAAGTTCAAGAGGAAAACTTATTATGCTCCAGCCATCTGATGATGTGACTTTTTGAATTGATGGTTTCTGGATTATACTATTTAGTTACTAAGGATTCAAGTTTCTTATCAAATCTTTGCCAAATTGCACTTGTAGAGTTGGGCTTTGTATATCTGATTCCATCCCTTATTCTGCTTCAACTTTCAATTGTGGATTGTTCCCCTTGACAGAAATATCTGCAATCAACAACTGTTAAAGTAGAGCAGATGAGAATTAGCAGAAGAGACGCCGAGCATTGGATGGCGCATCGTATGCTCCCCGAGGACTTGAGGCAGCGTATTAGACGATACGATCAGTACAAATGGCAGCTAAACAGGGGAGTTAGGGAAGAGGAACTCATCAGTAACCTTCCTAAAGACCTTAGGAGAGACATCAATCGCCATCTTTGCTTGGCTCTGCTTAAGAAAGTGAGTTCTTTCCTTCTACATTTTTCATATTTCTCCAATGAAATTGTAAATTTTCCTTATCTCGGAACGCCTTAGGTGCCACTTTTTAGCAGCATGGATAAGCAATTGCTGGATGCAATGTGCGAATATCTCAAGCCAGTGTTGTTCACAGAGAACAGCAACATTCTGCAAGAGGGTGACCCCATTGATATGATGTTGTTCATCATGAAAGGAAATCTGGCTACAATTATTACTTATGGTTGGAAAAACGACTCTCACTCGGACGTTCTCAAGGCTGGCGACTTCTGCGGTGAAGAGCTAGTTCCGTGGGCAATGGATCCGCAGTCCACTTCTCTTCCCATTTAAACCAGAACAGTCAAAACTCTAACTGAGGTTGAAGCCTTTGCTCTGAAGGCTAAAGAGCTGCAATCTGTGGTTAGTCAGTTCCACTTCCAGCGCTTCAATAGCCAGCAGTTCCAACTCTCAGTGAGGCATGCTCTTTCTACTGAACTCATACACAGATTTCTCTACCATAACGTTAGTTCAACCACTGATAAACTAAGTAACTGATTTGTAATCCTTGGTCTCTAGGTTCTACTCTCATCAATGGAAAGTTTGGGCTGCCTATAAAATACAAGAAGCTTGGCATGATTACTGTGAAAGAAAGCGGAGAGGGGGTGGAGATGGCAGGTTTCAAGATGCATTGGCTAAAACCGCTGGCGCCTCGGCGAGCTTTGGCGCCACTCTATATGCATCCATATTCATATCCCATCTACTGCAAGTTGTGCAGCGAGATCAACACCAGCAGACGTCCCAACTCTCAAGAGTGATGACGCTCCCACCGCCTCCGAAGCCGCTCGAGCAAGACAAGCGAGATTTCACCATCTTGGATCTTTGA

mRNA sequence

ATGGAAGCAGTTTTATGCTTCAAGCATGAATATGATCAGCCGAGGAAGAAACTGGAACTTTCGAAGAAAATTCTTGATCCACAGAAGCCATTTCTTCAGCAATGGAACAAGATATTTGTGCTCTCCTGTGTCCTAGCTGTAGCTGTGGACCCTCTCTTTTTTTACATCCCAGTGATTGATAGGCAACACCAATGCCTAACCATGGACCGGCTCCTTATGATCATTGCTTGCATTCTTCGTTCATTCATCGACATATTCTATCTACTTCACATTATCTTTGAATTCCGAACGGGCTTTCTACCTCCTTCTTTGCCAGTATTTGGAAGTGGCGAACTAGTTGAAGATCCAGCAGCTATAGCAAAGAGATACCTGTCCTCAAACTTCATAATTGATATCTTATCCATTATCCCTCTGCCTCAGCTTTTGGTATTAGTTATCATACCTGCAGCGAAAGGCCCGATTGCATTGAGGACAAAGGACTTCGTGAAGGTTGCGGTTCTTTTGCAGTACATTCCAAGGCTTCTCCGAATTTATCTGCTATACAGAGAAGTAACCAGAACTTCTGGCATACTGACAGAAAGGGCCTGGAGTGGAGCTGCCTTCAATCTTCTTATTTATATGCTAGCTAGTCATGTAGTTGGTGCCATTTGGTATTTGTTTTCAGTAGAACAAGAAGAAAGATGCTGGCTTGAGGCATGCAGAACACGTAACTGTAATAGCAAGGATCTGTGCTGTGGAGATCCCAGCAGAGCGGCATACATTATAAAGCAGTATTGCTCTCATAAAAAATCTGAAGATGACAAGACCTTCAACTTTGGAATGTATCTAGAAGCCCTTAAGTTTCATTTAACCGATCAGACGACGAATTTTCGCGACAAATTCTTTTATTGCTTCTGGTGGGCTCTGCGAAATGTCGGCTCTTGTGGACAGAACCTTCAAATAAGCAATTACTTGGGGGAGGTTCTTTTTGCTGTCTTCATTGCCATCCTTGGTCTGCTCTTGTTTGCGTTACTCATTAGCAATATTCAGAAATATCTGCAATCAACAACTGTTAAAGTAGAGCAGATGAGAATTAGCAGAAGAGACGCCGAGCATTGGATGGCGCATCGTATGCTCCCCGAGGACTTGAGGCAGCGTATTAGACGATACGATCAGTACAAATGGCAGCTAAACAGGGGAGTTAGGGAAGAGGAACTCATCAGTAACCTTCCTAAAGACCTTAGGAGAGACATCAATCGCCATCTTTGCTTGGCTCTGCTTAAGAAAGTGCCACTTTTTAGCAGCATGGATAAGCAATTGCTGGATGCAATGTGCGAATATCTCAAGCCAGTGTTGTTCACAGAGAACAGCAACATTCTGCAAGAGGGTGACCCCATTGATATGATGTTGTTCATCATGAAAGGAAATCTGGCTACAATTATTACTTATGGTTGGAAAAACGACTCTCACTCGGACGTTCTCAAGGCTGGCGACTTCTGCGGTGAAGAGCTAGTTCCGCTAAAGAGCTGCAATCTGTGGTTAGTCAGTTCCACTTCCAGCGCTTCAATAGCCAGCAGTTCCAACTCTCAGTTCTACTCTCATCAATGGAAAGTTTGGGCTGCCTATAAAATACAAGAAGCTTGGCATGATTACTGTGAAAGAAAGCGGAGAGGGGGTGGAGATGGCAGGTTTCAAGATGCATTGGCTAAAACCGCTGGCGCCTCGGCGAGCTTTGGCGCCACTCTATATGCATCCATATTCATATCCCATCTACTGCAAGTTGTGCAGCGAGATCAACACCAGCAGACGTCCCAACTCTCAAGAGTGATGACGCTCCCACCGCCTCCGAAGCCGCTCGAGCAAGACAAGCGAGATTTCACCATCTTGGATCTTTGA

Coding sequence (CDS)

ATGGAAGCAGTTTTATGCTTCAAGCATGAATATGATCAGCCGAGGAAGAAACTGGAACTTTCGAAGAAAATTCTTGATCCACAGAAGCCATTTCTTCAGCAATGGAACAAGATATTTGTGCTCTCCTGTGTCCTAGCTGTAGCTGTGGACCCTCTCTTTTTTTACATCCCAGTGATTGATAGGCAACACCAATGCCTAACCATGGACCGGCTCCTTATGATCATTGCTTGCATTCTTCGTTCATTCATCGACATATTCTATCTACTTCACATTATCTTTGAATTCCGAACGGGCTTTCTACCTCCTTCTTTGCCAGTATTTGGAAGTGGCGAACTAGTTGAAGATCCAGCAGCTATAGCAAAGAGATACCTGTCCTCAAACTTCATAATTGATATCTTATCCATTATCCCTCTGCCTCAGCTTTTGGTATTAGTTATCATACCTGCAGCGAAAGGCCCGATTGCATTGAGGACAAAGGACTTCGTGAAGGTTGCGGTTCTTTTGCAGTACATTCCAAGGCTTCTCCGAATTTATCTGCTATACAGAGAAGTAACCAGAACTTCTGGCATACTGACAGAAAGGGCCTGGAGTGGAGCTGCCTTCAATCTTCTTATTTATATGCTAGCTAGTCATGTAGTTGGTGCCATTTGGTATTTGTTTTCAGTAGAACAAGAAGAAAGATGCTGGCTTGAGGCATGCAGAACACGTAACTGTAATAGCAAGGATCTGTGCTGTGGAGATCCCAGCAGAGCGGCATACATTATAAAGCAGTATTGCTCTCATAAAAAATCTGAAGATGACAAGACCTTCAACTTTGGAATGTATCTAGAAGCCCTTAAGTTTCATTTAACCGATCAGACGACGAATTTTCGCGACAAATTCTTTTATTGCTTCTGGTGGGCTCTGCGAAATGTCGGCTCTTGTGGACAGAACCTTCAAATAAGCAATTACTTGGGGGAGGTTCTTTTTGCTGTCTTCATTGCCATCCTTGGTCTGCTCTTGTTTGCGTTACTCATTAGCAATATTCAGAAATATCTGCAATCAACAACTGTTAAAGTAGAGCAGATGAGAATTAGCAGAAGAGACGCCGAGCATTGGATGGCGCATCGTATGCTCCCCGAGGACTTGAGGCAGCGTATTAGACGATACGATCAGTACAAATGGCAGCTAAACAGGGGAGTTAGGGAAGAGGAACTCATCAGTAACCTTCCTAAAGACCTTAGGAGAGACATCAATCGCCATCTTTGCTTGGCTCTGCTTAAGAAAGTGCCACTTTTTAGCAGCATGGATAAGCAATTGCTGGATGCAATGTGCGAATATCTCAAGCCAGTGTTGTTCACAGAGAACAGCAACATTCTGCAAGAGGGTGACCCCATTGATATGATGTTGTTCATCATGAAAGGAAATCTGGCTACAATTATTACTTATGGTTGGAAAAACGACTCTCACTCGGACGTTCTCAAGGCTGGCGACTTCTGCGGTGAAGAGCTAGTTCCGCTAAAGAGCTGCAATCTGTGGTTAGTCAGTTCCACTTCCAGCGCTTCAATAGCCAGCAGTTCCAACTCTCAGTTCTACTCTCATCAATGGAAAGTTTGGGCTGCCTATAAAATACAAGAAGCTTGGCATGATTACTGTGAAAGAAAGCGGAGAGGGGGTGGAGATGGCAGGTTTCAAGATGCATTGGCTAAAACCGCTGGCGCCTCGGCGAGCTTTGGCGCCACTCTATATGCATCCATATTCATATCCCATCTACTGCAAGTTGTGCAGCGAGATCAACACCAGCAGACGTCCCAACTCTCAAGAGTGATGACGCTCCCACCGCCTCCGAAGCCGCTCGAGCAAGACAAGCGAGATTTCACCATCTTGGATCTTTGA

Protein sequence

MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDLCCGDPSRAAYIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSHSDVLKAGDFCGEELVPLKSCNLWLVSSTSSASIASSSNSQFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL
Homology
BLAST of MC05g_new0047 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 4.5e-176
Identity = 344/654 (52.60%), Postives = 448/654 (68.50%), Query Frame = 0

Query: 7   FKHEYDQPRKKLELSK------KILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 66
           FK  Y     K  +SK      KILDPQ PFLQ+WNKIFVL+C++AV++DPLFFY+P+ID
Sbjct: 61  FKRSYKSYSFKEAVSKGIGSTHKILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIID 120

Query: 67  RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 126
              +CL +D+ + I A +LRSF D+FY+LHIIF+FRTGF+ PS  VFG G LVED   IA
Sbjct: 121 DAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIA 180

Query: 127 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 186
           KRYLSS+FIIDIL+++PLPQ+++L+IIP  +G  +L TK+ +K  V  QYIPR +RIY L
Sbjct: 181 KRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNTKNMLKFIVFFQYIPRFIRIYPL 240

Query: 187 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRN--C 246
           Y+EVTRTSGILTE AW+GAAFNL +YMLASHV GA WYLFS+E+E  CW +AC   N  C
Sbjct: 241 YKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPC 300

Query: 247 NSKDLCCGDPSRAA--YIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFY 306
            SK L C DP  A     + + C   ++ +   F+FG++L+AL+  +  ++ +F  KFFY
Sbjct: 301 ISKLLYC-DPETAGGNAFLNESCP-IQTPNTTLFDFGIFLDALQSGVV-ESQDFPQKFFY 360

Query: 307 CFWWALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQM 366
           CFWW L+N+ S GQNL+ S Y+ E+ FAVFI+I GL+LF+ LI N+Q YLQSTT ++E+M
Sbjct: 361 CFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEM 420

Query: 367 RISRRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLC 426
           R+ RRDAE WM+HR+LPE+LR+RIRRY+QYKWQ  RGV EE L+SNLPKDLRRDI RHLC
Sbjct: 421 RVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLC 480

Query: 427 LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITY 486
           LALL +VP+F  MD+QLLDA+C+ L+PVL+TE S I++EGDP+D MLFIM+G L TI T 
Sbjct: 481 LALLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTN 540

Query: 487 GWKND-SHSDVLKAGDFCGEELV-----PLKSCNLWLVSSTSSA---------------S 546
           G +    +S+ L AGDFCGEEL+     P  S NL + + T  A                
Sbjct: 541 GGRTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPISTRTVRALMEVEAFALKADDLKF 600

Query: 547 IASS----------SNSQFYSHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALA 606
           +AS              ++YS QWK WAA  IQ AW  Y ++K        + R QDALA
Sbjct: 601 VASQFRRLHSKQLRHTFRYYSQQWKTWAACFIQAAWRRYIKKKLEESLKEEENRLQDALA 660

Query: 607 KTA-GASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQD 616
           K A G+S S GAT+YAS F +++L+ ++R    +  ++   M      KP E D
Sbjct: 661 KEACGSSPSLGATIYASRFAANILRTIRRSGSVRKPRMPERMPPMLLQKPAEPD 711

BLAST of MC05g_new0047 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 599.0 bits (1543), Expect = 6.3e-170
Identity = 325/642 (50.62%), Postives = 432/642 (67.29%), Query Frame = 0

Query: 22  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLTMDRLLMIIACILRS 81
           K I++PQ  FLQ WNKIF+ +CV+A+A+DPLFFYIP++D    CLT+D  L I A +LR+
Sbjct: 66  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 125

Query: 82  FIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQL 141
            ID FY++HI+F+FRT ++ PS  VFG GELV+D  AIA +YLSS FIID+LSI+PLPQ+
Sbjct: 126 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 185

Query: 142 LVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRTSGILTERAWSGAAF 201
           +VL +IP+   P++L TKD++K +++ QY+PR+LR+Y LY EVTRTSGI+TE AW+GAA+
Sbjct: 186 VVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAW 245

Query: 202 NLLIYMLASHVVGAIWYLFSVEQEERCWLEAC-RTRNCNSKDLCC-GDPSRAAYIIKQYC 261
           NL +YMLASHV GA+WYL SVE+E+RCW EAC +T+ CN K L C  D + +   +   C
Sbjct: 246 NLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSC 305

Query: 262 SHKKSED---DKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNVGSCGQNLQISN 321
                 D      FNFG++ +ALK  +  ++ +F  KFFYCFWW LRN+ + GQNLQ S 
Sbjct: 306 PFLDPGDITNSTIFNFGIFTDALKSGVV-ESHDFWKKFFYCFWWGLRNLSALGQNLQTSK 365

Query: 322 YLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEHWMAHRMLPEDL 381
           ++GE++FA+ I I GL+LFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLPEDL
Sbjct: 366 FVGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDL 425

Query: 382 RQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPLFSSMDKQLLDA 441
           R+RIRRY+QY+WQ  RGV EE L+ NLPKDLRRDI RHLCL LLKKVPLF  MD+QLLDA
Sbjct: 426 RKRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDA 485

Query: 442 MCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGE 501
           +C+ L+PVL+TENS +++EGDP+  MLF+M+G L +  T G ++   + V LKA DFCGE
Sbjct: 486 VCDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGE 545

Query: 502 ELVP--------------------LKSCNLWLVSSTSSASIAS----------SSNSQFY 561
           +L+P                    L     + +++    S+AS              +FY
Sbjct: 546 DLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFY 605

Query: 562 SHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALAK--------TAGASASFGAT 616
           S QW+ W+   IQ AW  YC RK        + R ++ALA         T  +S S G  
Sbjct: 606 SVQWRTWSVSFIQAAWRRYCRRKLAKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGA 665

BLAST of MC05g_new0047 vs. ExPASy Swiss-Prot
Match: Q9LD40 (Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 GN=CNGC13 PE=3 SV=2)

HSP 1 Score: 586.6 bits (1511), Expect = 3.2e-166
Identity = 321/637 (50.39%), Postives = 427/637 (67.03%), Query Frame = 0

Query: 22  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLTMDRLLMIIACILRS 81
           K I++PQ  FLQ WNKIF+ + V+A+A+DPLFFYIP++D +  CL + R L I A +LR+
Sbjct: 66  KNIINPQGSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRT 125

Query: 82  FIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQL 141
           FID FY++HI+F+FRT ++ PS  VFG GELV+DP AIA +YLSS FIID+LSI+PLPQL
Sbjct: 126 FIDAFYIIHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQL 185

Query: 142 LVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRTSGILTERAWSGAAF 201
           +VL +IP    P++L TKD++   +  QYIPR+LRIY LY EVTRTSGI+TE AW+GAA+
Sbjct: 186 VVLAVIPNVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAW 245

Query: 202 NLLIYMLASHVVGAIWYLFSVEQEERCWLEACR--TRNCNSKDLCC-GDPSRAAYIIKQY 261
           NL +YMLASHV GA+WYL SVE+E+RCW EAC      CN + L C G+ S     +   
Sbjct: 246 NLSLYMLASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTS 305

Query: 262 CSHKKSED---DKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNVGSCGQNLQIS 321
           C     +D      FNFG++ +ALK  +  ++ +F  KFFYCFWW LRN+ + GQNL  S
Sbjct: 306 CPFINPDDITNSTVFNFGIFTDALKSGIV-ESDDFWKKFFYCFWWGLRNLSALGQNLNTS 365

Query: 322 NYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEHWMAHRMLPED 381
            ++GE++FAV I I GL+LFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLP+D
Sbjct: 366 KFVGEIIFAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDD 425

Query: 382 LRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPLFSSMDKQLLD 441
           LR+RIRRY+QYKWQ  RGV EE L+ NLPKDLRRDI RH CL LLKKVPLF  MD+QLLD
Sbjct: 426 LRKRIRRYEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLD 485

Query: 442 AMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCG 501
           A+C+ LKPVL+TENS  ++EGDP++ MLF+M+G L +  T G +    + V LK  DFCG
Sbjct: 486 AVCDKLKPVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCG 545

Query: 502 EELV------------PLKSCNLWLVSSTSSASIASSS------------------NSQF 561
           E+L+            P+ +  +  ++   + ++A+                      +F
Sbjct: 546 EDLLTWALDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQFRRLHSKQLQHTFRF 605

Query: 562 YSHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALAK---TAGASASFGATLYAS 616
           YS QW+ W A  IQ AW  +C RK        + RF++A+ K    A +S+S  ATLYAS
Sbjct: 606 YSVQWRTWGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKRERNAASSSSLVATLYAS 665

BLAST of MC05g_new0047 vs. ExPASy Swiss-Prot
Match: Q9SKD7 (Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 GN=CNGC3 PE=2 SV=2)

HSP 1 Score: 547.4 bits (1409), Expect = 2.2e-154
Identity = 300/649 (46.22%), Postives = 427/649 (65.79%), Query Frame = 0

Query: 8   KHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLT 67
           K++ +  +KK+    ++++P   +LQ WNKIF+L  V+A+A DPLFFYIP +  +  CL 
Sbjct: 59  KNKENNKKKKI---LRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLN 118

Query: 68  MDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 127
           +D+ L  IAC+ R+FID FY++H++F+F TGF+ PS   FG GEL E    IA RYL S 
Sbjct: 119 LDKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRYLGSY 178

Query: 128 FIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRT 187
           F+ID+LSI+P+PQ++VL I+P  + P +L  K+ +K  +  QY+PR+ RIY L++EVTRT
Sbjct: 179 FLIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRT 238

Query: 188 SGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEAC-RTRNCNSKDLCC- 247
           SG++TE AW+GAA NL +YMLASHV G+ WYL S+E+++RCW EAC + +NC    L C 
Sbjct: 239 SGLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCS 298

Query: 248 --GDPSRAAYIIKQYC---SHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW 307
             G+ +R    +   C     ++  +   FNFG++ +AL+  +  ++ +F  KFFYCFWW
Sbjct: 299 PTGEDNR--LFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVV-ESRDFPKKFFYCFWW 358

Query: 308 ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR 367
            LRN+ + GQNL+ S + GE++FA+ I I GL+LFALLI N+QKYLQSTTV+VE+MR+ R
Sbjct: 359 GLRNLSALGQNLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKR 418

Query: 368 RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL 427
           RDAE WM+HRMLP+DLR+RIR+Y+QYKWQ  +GV EE L+S+LPKDLR+DI RHLCL LL
Sbjct: 419 RDAEQWMSHRMLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCLKLL 478

Query: 428 KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN 487
           KKVP F +MD +LLDA+C  LK VL+TE S I++EG+P++ MLFIM+GNL +  TYG + 
Sbjct: 479 KKVPWFQAMDDRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRT 538

Query: 488 DSHSDV-LKAGDFCGEELV----------PLKSCNL--------WLVSSTSSASIASSSN 547
              + V L AGDFCG+ L           P+ S  +        +L+S+     +A+   
Sbjct: 539 GFFNSVDLVAGDFCGDLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQYR 598

Query: 548 S----------QFYSHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALAK--TAG 607
                      +FYS QW+ WAA  IQ AW  +C RK        +G+  + L    + G
Sbjct: 599 RLHSKQLRHMFRFYSVQWQTWAACFIQAAWKRHCRRKLSKALREEEGKLHNTLQNDDSGG 658

Query: 608 ASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQD 616
              + GA +YAS F SH L+ ++ +   + S+   ++TL  P KP + +
Sbjct: 659 NKLNLGAAIYASRFASHALRNLRANAAARNSRFPHMLTL-LPQKPADPE 700

BLAST of MC05g_new0047 vs. ExPASy Swiss-Prot
Match: Q8RWS9 (Probable cyclic nucleotide-gated ion channel 5 OS=Arabidopsis thaliana OX=3702 GN=CNGC5 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 2.0e-152
Identity = 288/634 (45.43%), Postives = 402/634 (63.41%), Query Frame = 0

Query: 22  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLTMDRLLMIIACILRS 81
           KKI DPQ  FL   NK+FV SC+L+V VDP FFY+PVI+ + +CL +DR L I A  LR+
Sbjct: 86  KKIFDPQDKFLLYCNKLFVASCILSVFVDPFFFYLPVINAESKCLGIDRKLAITASTLRT 145

Query: 82  FIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQL 141
           FID+FYL H+  + RT ++ PS  VFG GELV DPA IAKRYL   FIID LS++PLPQ+
Sbjct: 146 FIDVFYLAHMALQLRTAYIAPSSRVFGRGELVIDPAQIAKRYLQRWFIIDFLSVLPLPQI 205

Query: 142 LVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRTSGILTERAWSGAAF 201
           +V   + ++ G   L TK  +   VL+QYIPR LR+  L  E+ RT+G+  E AW+GAA+
Sbjct: 206 VVWRFLQSSNGSDVLATKQALLFIVLVQYIPRFLRVLPLTSELKRTAGVFAETAWAGAAY 265

Query: 202 NLLIYMLASHVVGAIWYLFSVEQEERCWLEAC-RTRNCNSKDLCCGDPSRAAY------- 261
            LL+YMLASH+VGA WYL ++E+ + CW EAC    NC++  L CG+ +   Y       
Sbjct: 266 YLLLYMLASHIVGAFWYLLALERNDACWQEACIDAGNCSTDFLYCGNQNMDGYAVWNRAK 325

Query: 262 --IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNVGSCGQN 321
             ++K  C     +++  F+FG+Y +AL   +   + NF  K+ YC WW L+N+ + GQ 
Sbjct: 326 ESVLKSKCRADLDDNNPPFDFGIYTQALSSGIV-SSQNFIVKYCYCLWWGLQNLSTLGQG 385

Query: 322 LQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEHWMAHRM 381
           L+ S Y  E++F++ +AI GL+LFALLI N+Q YLQS T+++E+MR+ RRD+E WM HRM
Sbjct: 386 LETSTYPMEIIFSISLAISGLILFALLIGNMQTYLQSLTIRLEEMRVKRRDSEQWMHHRM 445

Query: 382 LPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPLFSSMDK 441
           LP+DLR+R+RRYDQYKW   RGV EE L+ NLPKDLRRDI RHLCLAL+++VPLF SMD 
Sbjct: 446 LPQDLRERVRRYDQYKWLETRGVDEEYLVQNLPKDLRRDIKRHLCLALVRRVPLFKSMDD 505

Query: 442 QLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKND-SHSDVLKAG 501
           +LLDA+C  LKP LFTE++ +++EGDP+D MLFI++G L ++ T G ++   +  +LK G
Sbjct: 506 KLLDAICMRLKPCLFTESTYLVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRSLLKEG 565

Query: 502 DFCGEELVPLKSCNLWLVSSTSSASIASSSNS---------------------------- 561
           +FCGEEL+       W +   S  ++ SS+ +                            
Sbjct: 566 EFCGEELL------TWALDPKSGVNLPSSTRTVKALTEVEAFALTSEELKFVASQFRRLH 625

Query: 562 --------QFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASASFGAT 609
                   +FYSHQW+ WAA  IQ AW  YC+RK+    +       + TAG S S GA 
Sbjct: 626 SRQVQHTFRFYSHQWRTWAACFIQAAWRRYCKRKKMEEAEAEAAAVSSSTAGPSYSIGAA 685

BLAST of MC05g_new0047 vs. NCBI nr
Match: XP_022147437.1 (LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 1177 bits (3044), Expect = 0.0
Identity = 606/655 (92.52%), Postives = 610/655 (93.13%), Query Frame = 0

Query: 1   MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 60
           MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID
Sbjct: 60  MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 119

Query: 61  RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 120
           RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA
Sbjct: 120 RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 179

Query: 121 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 180
           KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL
Sbjct: 180 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 239

Query: 181 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS 240
           YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS
Sbjct: 240 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS 299

Query: 241 KDLCCGDPSRAAYIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW 300
           KDLCCGDPSRAAYIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW
Sbjct: 300 KDLCCGDPSRAAYIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW 359

Query: 301 ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR 360
           ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR
Sbjct: 360 ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR 419

Query: 361 RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL 420
           RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL
Sbjct: 420 RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL 479

Query: 421 KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN 480
           KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN
Sbjct: 480 KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN 539

Query: 481 DSHSDVLKAGDFCGEELVP-------------------LKSCNLWLVSSTSSASIASSS- 540
           DSHSDVLKAGDFCGEELVP                   L     + + +    S+ S   
Sbjct: 540 DSHSDVLKAGDFCGEELVPWAMDPQSTSLPIXTRTVKTLTEVEAFALKAKELQSVVSQFH 599

Query: 541 ----NSQ-------FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASA 600
               NSQ       FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASA
Sbjct: 600 FQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASA 659

Query: 601 SFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 624
           SFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL
Sbjct: 660 SFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 714

BLAST of MC05g_new0047 vs. NCBI nr
Match: XP_022147438.1 (cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 1056 bits (2731), Expect = 0.0
Identity = 553/656 (84.30%), Postives = 572/656 (87.20%), Query Frame = 0

Query: 1   MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 60
           M A L FKHE DQ RKK E  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPV+D
Sbjct: 58  MVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMD 117

Query: 61  RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 120
           RQHQCLTMDRLLMIIAC+LRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA
Sbjct: 118 RQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 177

Query: 121 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 180
           KRYLSSNFIIDILSIIPLPQLL LVIIPAAKGPI L+TK+F+KVAVLLQY+PRL RIY L
Sbjct: 178 KRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPL 237

Query: 181 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS 240
           YREVTRTSGILTE AWSGAAFNLLIYMLASHVVGAIWYLFSVEQE RCW EACR  NCNS
Sbjct: 238 YREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKNNCNS 297

Query: 241 KDLCCGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFW 300
           K L CGD SR AY  IK+YC HK+SEDDKTFNFG+YLEALKF LTDQTTNF  KFFYCFW
Sbjct: 298 KYLYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFW 357

Query: 301 WALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRIS 360
           WALRNVGS GQNLQISNYLGE+ FAVFIAILGL+LFALLISNIQKYLQSTTVKVEQMRI+
Sbjct: 358 WALRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRIN 417

Query: 361 RRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLAL 420
           RRDAEHWM HRMLPEDLRQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDI RHLCLAL
Sbjct: 418 RRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLAL 477

Query: 421 LKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWK 480
           LKKVPLFSSMDKQLLDAMCEYLKPVLFTE S+ILQEGDP+DMMLFIMKGNLATIITYGWK
Sbjct: 478 LKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWK 537

Query: 481 NDSHSDVLKAGDFCGEELVP-------------------LKSCNLWLVSSTSSASIAS-- 540
           NDSHSDVLKAGDFCGEELVP                   L     + + +    S+ S  
Sbjct: 538 NDSHSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF 597

Query: 541 ---SSNSQ-------FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGAS 600
                NSQ       FYSHQWKVWAAYKIQEAWHDYCERKRRGGGD RFQ ALAKT GAS
Sbjct: 598 RFQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGAS 657

Query: 601 ASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 624
           ASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKP+E   RDFTILDL
Sbjct: 658 ASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIE---RDFTILDL 710

BLAST of MC05g_new0047 vs. NCBI nr
Match: XP_022924449.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_022924450.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >KAG6582669.1 Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 489/653 (74.89%), Postives = 535/653 (81.93%), Query Frame = 0

Query: 5   LCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQ 64
           L FKH  DQ  KK +  KKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPVI+ + Q
Sbjct: 58  LSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQ 117

Query: 65  CLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYL 124
           CLT+DR LMIIAC+LRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL
Sbjct: 118 CLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYL 177

Query: 125 SSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREV 184
            SNF+IDILS++PLPQLLVL IIPAA+GP+ L+TKD VK+AVLLQY+PRLLRIY LYREV
Sbjct: 178 FSNFLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREV 237

Query: 185 TRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDLC 244
           TRT GILTE AWSGAAFNLLIYM  SHVVGAIWYL SVE++ +CW+EACR   C    L 
Sbjct: 238 TRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLY 297

Query: 245 CGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALR 304
           CG+  R     I QYCS K+SED KTFNFGMY+EALKF LT  TT+FR KFFY FWWALR
Sbjct: 298 CGNQRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLTS-TTSFRRKFFYSFWWALR 357

Query: 305 NVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDA 364
           NV S GQNLQ+SNY+GEV FAVFIAILGL+LFALLISNIQKYLQS TVKVEQMRI+RRDA
Sbjct: 358 NVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDA 417

Query: 365 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKV 424
           EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGV+EEELI NLPKDLRRDI RHLC ALLKKV
Sbjct: 418 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKV 477

Query: 425 PLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSH 484
           PLFS MDKQLLDAMCEYLKPVLFTE S ILQEGDPIDM+LFIMKG LATIITYGWKND+ 
Sbjct: 478 PLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTL 537

Query: 485 SDVLKAGDFCGEELVP---------------------------LKSCNLWLVSSTSSASI 544
           S VL AGDFCGEELV                            LK+  L  V+S      
Sbjct: 538 SGVLNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQH 597

Query: 545 ASSSNSQ----FYSHQWKVWAAYKIQEAWHDYCERKRRG-GGDGRFQDALAKTAGASASF 604
            +S   Q    FYSHQW VWAAYK+QEAW DY ERKRRG  GDGRFQDALAKTAGAS  F
Sbjct: 598 LNSKQFQLSVRFYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDALAKTAGASVGF 657

Query: 605 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQ-DKRDFTILD 623
           GATLYASIFISHLLQVVQRDQHQ+T+QL++VMTLPPPPKP ++ D  +FTIL+
Sbjct: 658 GATLYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of MC05g_new0047 vs. NCBI nr
Match: XP_022980030.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980031.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980033.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980034.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 491/654 (75.08%), Postives = 533/654 (81.50%), Query Frame = 0

Query: 5   LCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQ 64
           L FKH  DQ  KK +  KKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPVID + Q
Sbjct: 58  LSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDGERQ 117

Query: 65  CLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYL 124
           CLT+DR LMIIAC+LRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL
Sbjct: 118 CLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYL 177

Query: 125 SSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREV 184
            SNF+IDILS++PLPQLLVL IIPAAKGPI L+TKD VK+AVLLQY+PRLLRIY LYREV
Sbjct: 178 LSNFLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREV 237

Query: 185 TRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDLC 244
           TRT GILTE AWSGAAFNLLIYM  SHVVGAIWYL SVE++ +CW+EACR   C    L 
Sbjct: 238 TRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLY 297

Query: 245 CGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALR 304
           CG+  R     I QYCS K+ ED KTFNFGMY+EALKF LT  TT+FR KFFY FWWALR
Sbjct: 298 CGNQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTS-TTSFRRKFFYSFWWALR 357

Query: 305 NVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDA 364
           NV S GQNLQ+SNY+GEV FAVFIAILGL+LFALLISNIQKYLQS TVKVEQMRI+RRDA
Sbjct: 358 NVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDA 417

Query: 365 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKV 424
           EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGV+EEELI NLPKDLRRDI RHLC ALLKKV
Sbjct: 418 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKV 477

Query: 425 PLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSH 484
           PLFS MDKQLLDAMCEYLKPVLFTE S ILQEGDPIDMMLFIMKG LATIITYGWKND+ 
Sbjct: 478 PLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKNDTF 537

Query: 485 SDVLKAGDFCGEELVP---------------------------LKSCNLWLVSSTSSASI 544
           S VL AGDFCGEELV                            LK+  L  V+S      
Sbjct: 538 SAVLNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQR 597

Query: 545 ASSSNSQ----FYSHQWKVWAAYKIQEAWHDYCERKRRG-GGDGRFQDALAKTAGASASF 604
            +S   Q    FYSHQW VWAAYKIQEAW +Y ERKRRG  GDGRFQ ALAKTAGAS  F
Sbjct: 598 LNSKQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQAALAKTAGASVGF 657

Query: 605 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQ-DKRDFTILDL 624
           GATLYASIFISHLLQVVQRDQH++T QL+RVMTLPPPPKP ++ D+ + TIL+L
Sbjct: 658 GATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVTILNL 710

BLAST of MC05g_new0047 vs. NCBI nr
Match: XP_023526877.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526878.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526879.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 485/651 (74.50%), Postives = 532/651 (81.72%), Query Frame = 0

Query: 7   FKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCL 66
           FKH  DQ  KK +  KKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPVI+ + QCL
Sbjct: 60  FKHPGDQSVKKPKFRKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCL 119

Query: 67  TMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSS 126
           T+DR LMIIAC+LRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL S
Sbjct: 120 TIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFS 179

Query: 127 NFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTR 186
           NF+IDILS++PLPQLLVL IIPAAKGPI L+TKD VK+AVLLQY+PRLLRIY LYREVTR
Sbjct: 180 NFLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTR 239

Query: 187 TSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDLCCG 246
           T GILTE AWSGAAFNLLIYM  SHVVGAIWYL SVE++ +CW+EACR   C    L CG
Sbjct: 240 TVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKGKCKDNFLYCG 299

Query: 247 DPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNV 306
           +  R  +  I QYCS K+SED+KTF FGM++EALKF LT  TT+FR KFFY FWWALRNV
Sbjct: 300 NQRRQIHPYIDQYCSPKESEDEKTFKFGMFVEALKFQLTS-TTSFRRKFFYSFWWALRNV 359

Query: 307 GSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEH 366
            S GQNLQ+SNY+G+V FAVFIAILGL+LFALLISNIQKYLQS TVKVEQMRI+RRDAEH
Sbjct: 360 SSSGQNLQVSNYMGDVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEH 419

Query: 367 WMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPL 426
           WMAHRMLPEDLRQRIRRYDQYKWQLNRGV+EEELI NLPKDLRRDI RHLC ALLKKVPL
Sbjct: 420 WMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPL 479

Query: 427 FSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSHSD 486
           FS MDKQLLDAMCEYLKPVLFTE S ILQEGDPIDMMLFIMKG LAT ITYGWKN++ S 
Sbjct: 480 FSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATSITYGWKNNTFSG 539

Query: 487 VLKAGDFCGEELVP---------------------------LKSCNLWLVSSTSSASIAS 546
           VL AGDFCGEELV                            LK+  L  V+S       +
Sbjct: 540 VLNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLN 599

Query: 547 SSNSQ----FYSHQWKVWAAYKIQEAWHDYCERKRRG-GGDGRFQDALAKTAGASASFGA 606
           S   Q    FYSHQW VWAAYKIQEAW +Y ERKRRG  GDGRFQDALAK AGAS  FGA
Sbjct: 600 SKQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQDALAKMAGASVGFGA 659

Query: 607 TLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQ-DKRDFTILD 623
           TLYASIFISHLLQVVQRDQ Q+T+QL+RVMTLPPPPKP ++ D  +FTIL+
Sbjct: 660 TLYASIFISHLLQVVQRDQPQETAQLTRVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of MC05g_new0047 vs. ExPASy TrEMBL
Match: A0A6J1D2C8 (LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111016362 PE=4 SV=1)

HSP 1 Score: 1177 bits (3044), Expect = 0.0
Identity = 606/655 (92.52%), Postives = 610/655 (93.13%), Query Frame = 0

Query: 1   MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 60
           MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID
Sbjct: 60  MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 119

Query: 61  RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 120
           RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA
Sbjct: 120 RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 179

Query: 121 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 180
           KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL
Sbjct: 180 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 239

Query: 181 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS 240
           YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS
Sbjct: 240 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS 299

Query: 241 KDLCCGDPSRAAYIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW 300
           KDLCCGDPSRAAYIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW
Sbjct: 300 KDLCCGDPSRAAYIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW 359

Query: 301 ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR 360
           ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR
Sbjct: 360 ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR 419

Query: 361 RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL 420
           RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL
Sbjct: 420 RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL 479

Query: 421 KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN 480
           KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN
Sbjct: 480 KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN 539

Query: 481 DSHSDVLKAGDFCGEELVP-------------------LKSCNLWLVSSTSSASIASSS- 540
           DSHSDVLKAGDFCGEELVP                   L     + + +    S+ S   
Sbjct: 540 DSHSDVLKAGDFCGEELVPWAMDPQSTSLPIXTRTVKTLTEVEAFALKAKELQSVVSQFH 599

Query: 541 ----NSQ-------FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASA 600
               NSQ       FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASA
Sbjct: 600 FQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASA 659

Query: 601 SFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 624
           SFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL
Sbjct: 660 SFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 714

BLAST of MC05g_new0047 vs. ExPASy TrEMBL
Match: A0A6J1D1B4 (cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111016363 PE=4 SV=1)

HSP 1 Score: 1056 bits (2731), Expect = 0.0
Identity = 553/656 (84.30%), Postives = 572/656 (87.20%), Query Frame = 0

Query: 1   MEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 60
           M A L FKHE DQ RKK E  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPV+D
Sbjct: 58  MVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMD 117

Query: 61  RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 120
           RQHQCLTMDRLLMIIAC+LRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA
Sbjct: 118 RQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 177

Query: 121 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 180
           KRYLSSNFIIDILSIIPLPQLL LVIIPAAKGPI L+TK+F+KVAVLLQY+PRL RIY L
Sbjct: 178 KRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPL 237

Query: 181 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNS 240
           YREVTRTSGILTE AWSGAAFNLLIYMLASHVVGAIWYLFSVEQE RCW EACR  NCNS
Sbjct: 238 YREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKNNCNS 297

Query: 241 KDLCCGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFW 300
           K L CGD SR AY  IK+YC HK+SEDDKTFNFG+YLEALKF LTDQTTNF  KFFYCFW
Sbjct: 298 KYLYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFW 357

Query: 301 WALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRIS 360
           WALRNVGS GQNLQISNYLGE+ FAVFIAILGL+LFALLISNIQKYLQSTTVKVEQMRI+
Sbjct: 358 WALRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRIN 417

Query: 361 RRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLAL 420
           RRDAEHWM HRMLPEDLRQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDI RHLCLAL
Sbjct: 418 RRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLAL 477

Query: 421 LKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWK 480
           LKKVPLFSSMDKQLLDAMCEYLKPVLFTE S+ILQEGDP+DMMLFIMKGNLATIITYGWK
Sbjct: 478 LKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWK 537

Query: 481 NDSHSDVLKAGDFCGEELVP-------------------LKSCNLWLVSSTSSASIAS-- 540
           NDSHSDVLKAGDFCGEELVP                   L     + + +    S+ S  
Sbjct: 538 NDSHSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF 597

Query: 541 ---SSNSQ-------FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGAS 600
                NSQ       FYSHQWKVWAAYKIQEAWHDYCERKRRGGGD RFQ ALAKT GAS
Sbjct: 598 RFQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGAS 657

Query: 601 ASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 624
           ASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKP+E   RDFTILDL
Sbjct: 658 ASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIE---RDFTILDL 710

BLAST of MC05g_new0047 vs. ExPASy TrEMBL
Match: A0A6J1EF20 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431950 PE=4 SV=1)

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 489/653 (74.89%), Postives = 535/653 (81.93%), Query Frame = 0

Query: 5   LCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQ 64
           L FKH  DQ  KK +  KKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPVI+ + Q
Sbjct: 58  LSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQ 117

Query: 65  CLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYL 124
           CLT+DR LMIIAC+LRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL
Sbjct: 118 CLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYL 177

Query: 125 SSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREV 184
            SNF+IDILS++PLPQLLVL IIPAA+GP+ L+TKD VK+AVLLQY+PRLLRIY LYREV
Sbjct: 178 FSNFLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREV 237

Query: 185 TRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDLC 244
           TRT GILTE AWSGAAFNLLIYM  SHVVGAIWYL SVE++ +CW+EACR   C    L 
Sbjct: 238 TRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLY 297

Query: 245 CGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALR 304
           CG+  R     I QYCS K+SED KTFNFGMY+EALKF LT  TT+FR KFFY FWWALR
Sbjct: 298 CGNQRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLTS-TTSFRRKFFYSFWWALR 357

Query: 305 NVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDA 364
           NV S GQNLQ+SNY+GEV FAVFIAILGL+LFALLISNIQKYLQS TVKVEQMRI+RRDA
Sbjct: 358 NVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDA 417

Query: 365 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKV 424
           EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGV+EEELI NLPKDLRRDI RHLC ALLKKV
Sbjct: 418 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKV 477

Query: 425 PLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSH 484
           PLFS MDKQLLDAMCEYLKPVLFTE S ILQEGDPIDM+LFIMKG LATIITYGWKND+ 
Sbjct: 478 PLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTL 537

Query: 485 SDVLKAGDFCGEELVP---------------------------LKSCNLWLVSSTSSASI 544
           S VL AGDFCGEELV                            LK+  L  V+S      
Sbjct: 538 SGVLNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQH 597

Query: 545 ASSSNSQ----FYSHQWKVWAAYKIQEAWHDYCERKRRG-GGDGRFQDALAKTAGASASF 604
            +S   Q    FYSHQW VWAAYK+QEAW DY ERKRRG  GDGRFQDALAKTAGAS  F
Sbjct: 598 LNSKQFQLSVRFYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDALAKTAGASVGF 657

Query: 605 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQ-DKRDFTILD 623
           GATLYASIFISHLLQVVQRDQHQ+T+QL++VMTLPPPPKP ++ D  +FTIL+
Sbjct: 658 GATLYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of MC05g_new0047 vs. ExPASy TrEMBL
Match: A0A6J1IV06 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479553 PE=4 SV=1)

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 491/654 (75.08%), Postives = 533/654 (81.50%), Query Frame = 0

Query: 5   LCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQ 64
           L FKH  DQ  KK +  KKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPVID + Q
Sbjct: 58  LSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDGERQ 117

Query: 65  CLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYL 124
           CLT+DR LMIIAC+LRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL
Sbjct: 118 CLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYL 177

Query: 125 SSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREV 184
            SNF+IDILS++PLPQLLVL IIPAAKGPI L+TKD VK+AVLLQY+PRLLRIY LYREV
Sbjct: 178 LSNFLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREV 237

Query: 185 TRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDLC 244
           TRT GILTE AWSGAAFNLLIYM  SHVVGAIWYL SVE++ +CW+EACR   C    L 
Sbjct: 238 TRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLY 297

Query: 245 CGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALR 304
           CG+  R     I QYCS K+ ED KTFNFGMY+EALKF LT  TT+FR KFFY FWWALR
Sbjct: 298 CGNQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTS-TTSFRRKFFYSFWWALR 357

Query: 305 NVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDA 364
           NV S GQNLQ+SNY+GEV FAVFIAILGL+LFALLISNIQKYLQS TVKVEQMRI+RRDA
Sbjct: 358 NVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDA 417

Query: 365 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKV 424
           EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGV+EEELI NLPKDLRRDI RHLC ALLKKV
Sbjct: 418 EHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKV 477

Query: 425 PLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSH 484
           PLFS MDKQLLDAMCEYLKPVLFTE S ILQEGDPIDMMLFIMKG LATIITYGWKND+ 
Sbjct: 478 PLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKNDTF 537

Query: 485 SDVLKAGDFCGEELVP---------------------------LKSCNLWLVSSTSSASI 544
           S VL AGDFCGEELV                            LK+  L  V+S      
Sbjct: 538 SAVLNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQR 597

Query: 545 ASSSNSQ----FYSHQWKVWAAYKIQEAWHDYCERKRRG-GGDGRFQDALAKTAGASASF 604
            +S   Q    FYSHQW VWAAYKIQEAW +Y ERKRRG  GDGRFQ ALAKTAGAS  F
Sbjct: 598 LNSKQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQAALAKTAGASVGF 657

Query: 605 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQ-DKRDFTILDL 624
           GATLYASIFISHLLQVVQRDQH++T QL+RVMTLPPPPKP ++ D+ + TIL+L
Sbjct: 658 GATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVTILNL 710

BLAST of MC05g_new0047 vs. ExPASy TrEMBL
Match: A0A1S3AUR7 (cyclic nucleotide-gated ion channel 1 OS=Cucumis melo OX=3656 GN=LOC103483195 PE=4 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 475/658 (72.19%), Postives = 528/658 (80.24%), Query Frame = 0

Query: 4   VLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQH 63
           +L FKH  DQ  KK +  KKILDP KPFLQ+WNKIFVLS V+AVAVDPLFFY+PV D + 
Sbjct: 50  ILAFKHSGDQSVKKPKFWKKILDPHKPFLQRWNKIFVLSSVIAVAVDPLFFYVPVFDGKD 109

Query: 64  QCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 123
           QCL MDR L+IIAC+LRSFID+FYLLH+IFEFRTGFLPPSLPVFGSGEL++DP  IAK+Y
Sbjct: 110 QCLAMDRQLIIIACVLRSFIDLFYLLHMIFEFRTGFLPPSLPVFGSGELIKDPVKIAKKY 169

Query: 124 LSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYRE 183
           L SNF+ID LSIIPLPQLLVL IIPA KGPI L+TKD VK+AVLLQYIPRLLRIY LYRE
Sbjct: 170 LFSNFLIDFLSIIPLPQLLVLAIIPAVKGPIPLKTKDAVKMAVLLQYIPRLLRIYPLYRE 229

Query: 184 VTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTR--NCNSK 243
           VTRTSGILTE AWSGAAFNLLIYMLASHVVGAIWYL S+E++ +CW++AC+    NC +K
Sbjct: 230 VTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLLSIERQAKCWVQACKEDDINCTAK 289

Query: 244 DLCCGDPSRAAY-IIKQYCSHKKSEDDKT-FNFGMYLEALKFHLTDQTTNFRDKFFYCFW 303
            L CG+  R AY  I + C  K SED K  F FG+Y EALKF+LTD T +FR KF Y FW
Sbjct: 290 FLYCGNHKRNAYPSINKTCFPKDSEDGKDGFEFGIYAEALKFNLTD-TMSFRRKFCYSFW 349

Query: 304 WALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRIS 363
           WALRNVGS GQNL++S+Y+GEV FAVFIAILGL+LFA LISNIQKYLQS TVKVEQMRI+
Sbjct: 350 WALRNVGSSGQNLEVSHYMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKVEQMRIN 409

Query: 364 RRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLAL 423
           RRDAEHWMAHRMLP++LR RIRRYDQYKWQLNRGV+EEELISNLPKDLRRDI RHLCLA 
Sbjct: 410 RRDAEHWMAHRMLPDELRYRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLAQ 469

Query: 424 LKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWK 483
           LKKVPLFSSMDKQLLDAMCEYL+PVLFTE S I+QEGDPIDMMLFIMKG LATII   WK
Sbjct: 470 LKKVPLFSSMDKQLLDAMCEYLRPVLFTEKSFIMQEGDPIDMMLFIMKGKLATIIGCDWK 529

Query: 484 NDSHSDVLKAGDFCGEELV-----------------------------PLKSCNLWLVSS 543
           ND +S  L AGDFCGEELV                              LK+  L  V+S
Sbjct: 530 NDLYSATLNAGDFCGEELVHWAMDPTTNTSSLPISKRTVETLTEVEAFALKANELKFVTS 589

Query: 544 TSSASIASSSNSQF----YSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAG 603
              +   +S + QF    +SHQW VWAAYKIQEAWHDY ERK+RGGGDGRFQDA AK  G
Sbjct: 590 QFHSQQLNSKHFQFSVRFHSHQWNVWAAYKIQEAWHDYRERKQRGGGDGRFQDAFAKIVG 649

Query: 604 ASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPL-EQDKRDFTILD 623
            S SF ATLYASIFISHLLQV+QR+Q QQT Q++RVMTLPPPPKP  EQ+K DFTILD
Sbjct: 650 PSTSFIATLYASIFISHLLQVMQRNQPQQTPQVTRVMTLPPPPKPEDEQNKADFTILD 706

BLAST of MC05g_new0047 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 619.4 bits (1596), Expect = 3.2e-177
Identity = 344/654 (52.60%), Postives = 448/654 (68.50%), Query Frame = 0

Query: 7   FKHEYDQPRKKLELSK------KILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVID 66
           FK  Y     K  +SK      KILDPQ PFLQ+WNKIFVL+C++AV++DPLFFY+P+ID
Sbjct: 61  FKRSYKSYSFKEAVSKGIGSTHKILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIID 120

Query: 67  RQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIA 126
              +CL +D+ + I A +LRSF D+FY+LHIIF+FRTGF+ PS  VFG G LVED   IA
Sbjct: 121 DAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIA 180

Query: 127 KRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLL 186
           KRYLSS+FIIDIL+++PLPQ+++L+IIP  +G  +L TK+ +K  V  QYIPR +RIY L
Sbjct: 181 KRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNTKNMLKFIVFFQYIPRFIRIYPL 240

Query: 187 YREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRN--C 246
           Y+EVTRTSGILTE AW+GAAFNL +YMLASHV GA WYLFS+E+E  CW +AC   N  C
Sbjct: 241 YKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPC 300

Query: 247 NSKDLCCGDPSRAA--YIIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFY 306
            SK L C DP  A     + + C   ++ +   F+FG++L+AL+  +  ++ +F  KFFY
Sbjct: 301 ISKLLYC-DPETAGGNAFLNESCP-IQTPNTTLFDFGIFLDALQSGVV-ESQDFPQKFFY 360

Query: 307 CFWWALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQM 366
           CFWW L+N+ S GQNL+ S Y+ E+ FAVFI+I GL+LF+ LI N+Q YLQSTT ++E+M
Sbjct: 361 CFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEM 420

Query: 367 RISRRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLC 426
           R+ RRDAE WM+HR+LPE+LR+RIRRY+QYKWQ  RGV EE L+SNLPKDLRRDI RHLC
Sbjct: 421 RVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLC 480

Query: 427 LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITY 486
           LALL +VP+F  MD+QLLDA+C+ L+PVL+TE S I++EGDP+D MLFIM+G L TI T 
Sbjct: 481 LALLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTN 540

Query: 487 GWKND-SHSDVLKAGDFCGEELV-----PLKSCNLWLVSSTSSA---------------S 546
           G +    +S+ L AGDFCGEEL+     P  S NL + + T  A                
Sbjct: 541 GGRTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPISTRTVRALMEVEAFALKADDLKF 600

Query: 547 IASS----------SNSQFYSHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALA 606
           +AS              ++YS QWK WAA  IQ AW  Y ++K        + R QDALA
Sbjct: 601 VASQFRRLHSKQLRHTFRYYSQQWKTWAACFIQAAWRRYIKKKLEESLKEEENRLQDALA 660

Query: 607 KTA-GASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQD 616
           K A G+S S GAT+YAS F +++L+ ++R    +  ++   M      KP E D
Sbjct: 661 KEACGSSPSLGATIYASRFAANILRTIRRSGSVRKPRMPERMPPMLLQKPAEPD 711

BLAST of MC05g_new0047 vs. TAIR 10
Match: AT1G01340.1 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 599.0 bits (1543), Expect = 4.4e-171
Identity = 325/642 (50.62%), Postives = 432/642 (67.29%), Query Frame = 0

Query: 22  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLTMDRLLMIIACILRS 81
           K I++PQ  FLQ WNKIF+ +CV+A+A+DPLFFYIP++D    CLT+D  L I A +LR+
Sbjct: 61  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 120

Query: 82  FIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQL 141
            ID FY++HI+F+FRT ++ PS  VFG GELV+D  AIA +YLSS FIID+LSI+PLPQ+
Sbjct: 121 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 180

Query: 142 LVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRTSGILTERAWSGAAF 201
           +VL +IP+   P++L TKD++K +++ QY+PR+LR+Y LY EVTRTSGI+TE AW+GAA+
Sbjct: 181 VVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAW 240

Query: 202 NLLIYMLASHVVGAIWYLFSVEQEERCWLEAC-RTRNCNSKDLCC-GDPSRAAYIIKQYC 261
           NL +YMLASHV GA+WYL SVE+E+RCW EAC +T+ CN K L C  D + +   +   C
Sbjct: 241 NLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSC 300

Query: 262 SHKKSED---DKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNVGSCGQNLQISN 321
                 D      FNFG++ +ALK  +  ++ +F  KFFYCFWW LRN+ + GQNLQ S 
Sbjct: 301 PFLDPGDITNSTIFNFGIFTDALKSGVV-ESHDFWKKFFYCFWWGLRNLSALGQNLQTSK 360

Query: 322 YLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEHWMAHRMLPEDL 381
           ++GE++FA+ I I GL+LFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLPEDL
Sbjct: 361 FVGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDL 420

Query: 382 RQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPLFSSMDKQLLDA 441
           R+RIRRY+QY+WQ  RGV EE L+ NLPKDLRRDI RHLCL LLKKVPLF  MD+QLLDA
Sbjct: 421 RKRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDA 480

Query: 442 MCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGE 501
           +C+ L+PVL+TENS +++EGDP+  MLF+M+G L +  T G ++   + V LKA DFCGE
Sbjct: 481 VCDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGE 540

Query: 502 ELVP--------------------LKSCNLWLVSSTSSASIAS----------SSNSQFY 561
           +L+P                    L     + +++    S+AS              +FY
Sbjct: 541 DLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFY 600

Query: 562 SHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALAK--------TAGASASFGAT 616
           S QW+ W+   IQ AW  YC RK        + R ++ALA         T  +S S G  
Sbjct: 601 SVQWRTWSVSFIQAAWRRYCRRKLAKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGA 660

BLAST of MC05g_new0047 vs. TAIR 10
Match: AT1G01340.2 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 599.0 bits (1543), Expect = 4.4e-171
Identity = 325/642 (50.62%), Postives = 432/642 (67.29%), Query Frame = 0

Query: 22  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLTMDRLLMIIACILRS 81
           K I++PQ  FLQ WNKIF+ +CV+A+A+DPLFFYIP++D    CLT+D  L I A +LR+
Sbjct: 66  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 125

Query: 82  FIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQL 141
            ID FY++HI+F+FRT ++ PS  VFG GELV+D  AIA +YLSS FIID+LSI+PLPQ+
Sbjct: 126 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 185

Query: 142 LVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRTSGILTERAWSGAAF 201
           +VL +IP+   P++L TKD++K +++ QY+PR+LR+Y LY EVTRTSGI+TE AW+GAA+
Sbjct: 186 VVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAW 245

Query: 202 NLLIYMLASHVVGAIWYLFSVEQEERCWLEAC-RTRNCNSKDLCC-GDPSRAAYIIKQYC 261
           NL +YMLASHV GA+WYL SVE+E+RCW EAC +T+ CN K L C  D + +   +   C
Sbjct: 246 NLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSC 305

Query: 262 SHKKSED---DKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNVGSCGQNLQISN 321
                 D      FNFG++ +ALK  +  ++ +F  KFFYCFWW LRN+ + GQNLQ S 
Sbjct: 306 PFLDPGDITNSTIFNFGIFTDALKSGVV-ESHDFWKKFFYCFWWGLRNLSALGQNLQTSK 365

Query: 322 YLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEHWMAHRMLPEDL 381
           ++GE++FA+ I I GL+LFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLPEDL
Sbjct: 366 FVGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDL 425

Query: 382 RQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPLFSSMDKQLLDA 441
           R+RIRRY+QY+WQ  RGV EE L+ NLPKDLRRDI RHLCL LLKKVPLF  MD+QLLDA
Sbjct: 426 RKRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDA 485

Query: 442 MCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGE 501
           +C+ L+PVL+TENS +++EGDP+  MLF+M+G L +  T G ++   + V LKA DFCGE
Sbjct: 486 VCDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGE 545

Query: 502 ELVP--------------------LKSCNLWLVSSTSSASIAS----------SSNSQFY 561
           +L+P                    L     + +++    S+AS              +FY
Sbjct: 546 DLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFY 605

Query: 562 SHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALAK--------TAGASASFGAT 616
           S QW+ W+   IQ AW  YC RK        + R ++ALA         T  +S S G  
Sbjct: 606 SVQWRTWSVSFIQAAWRRYCRRKLAKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGA 665

BLAST of MC05g_new0047 vs. TAIR 10
Match: AT4G01010.1 (cyclic nucleotide-gated channel 13 )

HSP 1 Score: 586.6 bits (1511), Expect = 2.3e-167
Identity = 321/637 (50.39%), Postives = 427/637 (67.03%), Query Frame = 0

Query: 22  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLTMDRLLMIIACILRS 81
           K I++PQ  FLQ WNKIF+ + V+A+A+DPLFFYIP++D +  CL + R L I A +LR+
Sbjct: 66  KNIINPQGSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRT 125

Query: 82  FIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQL 141
           FID FY++HI+F+FRT ++ PS  VFG GELV+DP AIA +YLSS FIID+LSI+PLPQL
Sbjct: 126 FIDAFYIIHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQL 185

Query: 142 LVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRTSGILTERAWSGAAF 201
           +VL +IP    P++L TKD++   +  QYIPR+LRIY LY EVTRTSGI+TE AW+GAA+
Sbjct: 186 VVLAVIPNVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAW 245

Query: 202 NLLIYMLASHVVGAIWYLFSVEQEERCWLEACR--TRNCNSKDLCC-GDPSRAAYIIKQY 261
           NL +YMLASHV GA+WYL SVE+E+RCW EAC      CN + L C G+ S     +   
Sbjct: 246 NLSLYMLASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTS 305

Query: 262 CSHKKSED---DKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWALRNVGSCGQNLQIS 321
           C     +D      FNFG++ +ALK  +  ++ +F  KFFYCFWW LRN+ + GQNL  S
Sbjct: 306 CPFINPDDITNSTVFNFGIFTDALKSGIV-ESDDFWKKFFYCFWWGLRNLSALGQNLNTS 365

Query: 322 NYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRDAEHWMAHRMLPED 381
            ++GE++FAV I I GL+LFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLP+D
Sbjct: 366 KFVGEIIFAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDD 425

Query: 382 LRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKKVPLFSSMDKQLLD 441
           LR+RIRRY+QYKWQ  RGV EE L+ NLPKDLRRDI RH CL LLKKVPLF  MD+QLLD
Sbjct: 426 LRKRIRRYEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLD 485

Query: 442 AMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCG 501
           A+C+ LKPVL+TENS  ++EGDP++ MLF+M+G L +  T G +    + V LK  DFCG
Sbjct: 486 AVCDKLKPVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCG 545

Query: 502 EELV------------PLKSCNLWLVSSTSSASIASSS------------------NSQF 561
           E+L+            P+ +  +  ++   + ++A+                      +F
Sbjct: 546 EDLLTWALDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQFRRLHSKQLQHTFRF 605

Query: 562 YSHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALAK---TAGASASFGATLYAS 616
           YS QW+ W A  IQ AW  +C RK        + RF++A+ K    A +S+S  ATLYAS
Sbjct: 606 YSVQWRTWGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKRERNAASSSSLVATLYAS 665

BLAST of MC05g_new0047 vs. TAIR 10
Match: AT2G46430.1 (cyclic nucleotide gated channel 3 )

HSP 1 Score: 547.4 bits (1409), Expect = 1.5e-155
Identity = 300/649 (46.22%), Postives = 427/649 (65.79%), Query Frame = 0

Query: 8   KHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQHQCLT 67
           K++ +  +KK+    ++++P   +LQ WNKIF+L  V+A+A DPLFFYIP +  +  CL 
Sbjct: 59  KNKENNKKKKI---LRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLN 118

Query: 68  MDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 127
           +D+ L  IAC+ R+FID FY++H++F+F TGF+ PS   FG GEL E    IA RYL S 
Sbjct: 119 LDKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRYLGSY 178

Query: 128 FIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYREVTRT 187
           F+ID+LSI+P+PQ++VL I+P  + P +L  K+ +K  +  QY+PR+ RIY L++EVTRT
Sbjct: 179 FLIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRT 238

Query: 188 SGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEAC-RTRNCNSKDLCC- 247
           SG++TE AW+GAA NL +YMLASHV G+ WYL S+E+++RCW EAC + +NC    L C 
Sbjct: 239 SGLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCS 298

Query: 248 --GDPSRAAYIIKQYC---SHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWW 307
             G+ +R    +   C     ++  +   FNFG++ +AL+  +  ++ +F  KFFYCFWW
Sbjct: 299 PTGEDNR--LFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVV-ESRDFPKKFFYCFWW 358

Query: 308 ALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISR 367
            LRN+ + GQNL+ S + GE++FA+ I I GL+LFALLI N+QKYLQSTTV+VE+MR+ R
Sbjct: 359 GLRNLSALGQNLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKR 418

Query: 368 RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALL 427
           RDAE WM+HRMLP+DLR+RIR+Y+QYKWQ  +GV EE L+S+LPKDLR+DI RHLCL LL
Sbjct: 419 RDAEQWMSHRMLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCLKLL 478

Query: 428 KKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKN 487
           KKVP F +MD +LLDA+C  LK VL+TE S I++EG+P++ MLFIM+GNL +  TYG + 
Sbjct: 479 KKVPWFQAMDDRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRT 538

Query: 488 DSHSDV-LKAGDFCGEELV----------PLKSCNL--------WLVSSTSSASIASSSN 547
              + V L AGDFCG+ L           P+ S  +        +L+S+     +A+   
Sbjct: 539 GFFNSVDLVAGDFCGDLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQYR 598

Query: 548 S----------QFYSHQWKVWAAYKIQEAWHDYCERKRRGG---GDGRFQDALAK--TAG 607
                      +FYS QW+ WAA  IQ AW  +C RK        +G+  + L    + G
Sbjct: 599 RLHSKQLRHMFRFYSVQWQTWAACFIQAAWKRHCRRKLSKALREEEGKLHNTLQNDDSGG 658

Query: 608 ASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQD 616
              + GA +YAS F SH L+ ++ +   + S+   ++TL  P KP + +
Sbjct: 659 NKLNLGAAIYASRFASHALRNLRANAAARNSRFPHMLTL-LPQKPADPE 700

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O657174.5e-17652.60Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 P... [more]
Q9LNJ06.3e-17050.62Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LD403.2e-16650.39Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SKD72.2e-15446.22Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8RWS92.0e-15245.43Probable cyclic nucleotide-gated ion channel 5 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
XP_022147437.10.092.52LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like [Momordica chara... [more]
XP_022147438.10.084.30cyclic nucleotide-gated ion channel 1-like [Momordica charantia][more]
XP_022924449.10.074.89cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_0... [more]
XP_022980030.10.075.08cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022... [more]
XP_023526877.10.074.50cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
A0A6J1D2C80.092.52LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like OS=Momordica cha... [more]
A0A6J1D1B40.084.30cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1EF200.074.89cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1IV060.075.08cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A1S3AUR70.072.19cyclic nucleotide-gated ion channel 1 OS=Cucumis melo OX=3656 GN=LOC103483195 PE... [more]
Match NameE-valueIdentityDescription
AT5G53130.13.2e-17752.60cyclic nucleotide gated channel 1 [more]
AT1G01340.14.4e-17150.62cyclic nucleotide gated channel 10 [more]
AT1G01340.24.4e-17150.62cyclic nucleotide gated channel 10 [more]
AT4G01010.12.3e-16750.39cyclic nucleotide-gated channel 13 [more]
AT2G46430.11.5e-15546.22cyclic nucleotide gated channel 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000595Cyclic nucleotide-binding domainSMARTSM00100cnmp_10coord: 425..543
e-value: 8.6E-4
score: 28.6
IPR000595Cyclic nucleotide-binding domainPROSITEPS50042CNMP_BINDING_3coord: 425..526
score: 11.7666
IPR000595Cyclic nucleotide-binding domainCDDcd00038CAP_EDcoord: 425..498
e-value: 7.88628E-11
score: 57.721
IPR005821Ion transport domainPFAMPF00520Ion_transcoord: 34..353
e-value: 3.7E-34
score: 118.1
NoneNo IPR availableGENE3D1.10.287.630Helix hairpin bincoord: 351..416
e-value: 7.0E-18
score: 66.7
NoneNo IPR availableGENE3D1.10.287.70coord: 37..349
e-value: 3.1E-12
score: 48.4
NoneNo IPR availablePANTHERPTHR45651:SF5CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1coord: 12..499
coord: 518..606
NoneNo IPR availablePANTHERPTHR45651CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATEDcoord: 12..499
coord: 518..606
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 26..347
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 423..536
e-value: 1.1E-21
score: 78.8
IPR018490Cyclic nucleotide-binding-likeSUPERFAMILY51206cAMP-binding domain-likecoord: 355..498

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g_new0047.1MC05g_new0047.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005216 ion channel activity