MC05g1516 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1516 vs. ExPASy Swiss-Prot
Match: Q9M394 (Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.6e-192
Identity = 343/482 (71.16%), Postives = 393/482 (81.54%), Query Frame = 0

Query: 187 SLRTNEVMIPNSSKSPR--LNPARASVGDDG--NGVV--ETASPTRRGRKKGMTKSSSSS 246
           SL   EV++   S++ R  L P +AS+   G  NG     T+ P+R+GRKK  T      
Sbjct: 16  SLDRREVLVVRHSQASRRFLTP-KASINGSGITNGAAAETTSKPSRKGRKKKQT------ 75

Query: 247 TTTQKKTKRSKKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGA 306
                       T++E + TE +P         ++     YDDG++FPY+DPPL+CCFGA
Sbjct: 76  -----------STVIEKDNTETDP--------ELNPELADYDDGIEFPYDDPPLVCCFGA 135

Query: 307 AQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMG 366
            QKEFVP VRVHDN MH D YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMG
Sbjct: 136 VQKEFVPVVRVHDNPMHPDMYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMG 195

Query: 367 KVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSL 426
           KVG+DDFGDELVLMMN+E+VQTRAVKFD NS+TACT +KIKF+DGK+  ETVKE  EDSL
Sbjct: 196 KVGEDDFGDELVLMMNQERVQTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSL 255

Query: 427 LSSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSR 486
            +SELNL+VLKEARIFHFNSE L S  M+STLF AIQ SKKFGGLIFFDLNL LPLWRSR
Sbjct: 256 FASELNLAVLKEARIFHFNSEVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSR 315

Query: 487 DETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHY 546
           +ETR+ IKKAWNEA+IIEVS+QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRDYYHY
Sbjct: 316 NETRKLIKKAWNEANIIEVSQQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHY 375

Query: 547 TPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVA 606
           TPEEI  LWHD+L+LL VTDGT+R+HYY+P+F GVVIGTEDVLITPFTCDRTGSGDAVVA
Sbjct: 376 TPEEIKSLWHDKLKLLVVTDGTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVA 435

Query: 607 GIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPS 663
           GIMRKLTT PEMFE+QDV+ERQLRFA+AAGII+QWTIGAVRGFPTESATQNLKEQVYVPS
Sbjct: 436 GIMRKLTTCPEMFEDQDVMERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPS 471

BLAST of MC05g1516 vs. ExPASy Swiss-Prot
Match: F4I0K2 (Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 SV=2)

HSP 1 Score: 325.5 bits (833), Expect = 1.4e-87
Identity = 184/451 (40.80%), Postives = 269/451 (59.65%), Query Frame = 0

Query: 214 DGNGVVETASPTR-RGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGN--------- 273
           D   +V    P + R RKK    SS       +K  R K+T+ +D+  E +         
Sbjct: 107 DKESIVSALKPKKTRTRKKAAAASSDVEEVKTEKKVRRKRTVKKDKDVEDDLATIMDAEV 166

Query: 274 ----------PFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHD 333
                       +  SE+E ID +++   + +   Y  PPL+CCFG+AQ  FVPS R  +
Sbjct: 167 SDVEEALAVESTDTESEEEEID-LSKHEGEDISHTYGWPPLVCCFGSAQHAFVPSGRPAN 226

Query: 334 NQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVL 393
             +  + +   +  +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++ 
Sbjct: 227 RLLDYELHERMRDAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAMLY 286

Query: 394 MMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEA 453
            +N  KVQTR+VK D    TAC+ MKI  + G+LK   +K  AEDSL  SE+N+ VLKEA
Sbjct: 287 YLNVCKVQTRSVKIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKEA 346

Query: 454 RIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNE 513
           ++F+F++ +L+   M ST  +AI++SK+ G +IF+DLNL LPLW S +ET+ +I++ WN 
Sbjct: 347 KMFYFSTHSLLDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNL 406

Query: 514 ADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRL 573
           AD+IE+++QELEFL   E  E       ++  +  + +K    + HY PE +  LWH+ L
Sbjct: 407 ADVIEITKQELEFLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHENL 466

Query: 574 RLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMF 633
           ++LFVT+GT +IHYY+   +G V G EDV ITPFT D + SGD +VAG++R LT  P++ 
Sbjct: 467 KVLFVTNGTSKIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLM 526

Query: 634 ENQDVLERQLRFAIAAGIISQWTIGAVRGFP 645
            N+  LER  R+AI  GII QW +   RG+P
Sbjct: 527 NNKGYLERTARYAIECGIIDQWLLAQTRGYP 544

BLAST of MC05g1516 vs. ExPASy Swiss-Prot
Match: Q42896 (Fructokinase-2 OS=Solanum lycopersicum OX=4081 GN=FRK2 PE=2 SV=2)

HSP 1 Score: 193.7 bits (491), Expect = 6.4e-48
Identity = 122/360 (33.89%), Postives = 186/360 (51.67%), Query Frame = 0

Query: 294 LICCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLG 353
           LI  FG    +FVP+V                +   + P F +APGGAP+NVAI+  RLG
Sbjct: 11  LIVSFGEMLIDFVPTVS--------------GVSLAEAPGFLKAPGGAPANVAIAVTRLG 70

Query: 354 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVK 413
           G++AF+GK+G D+FG  L  ++    VQ   + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 71  GKSAFVGKLGDDEFGHMLAGILKTNGVQAEGINFDKGARTALAFVTLR-ADGEREFMFYR 130

Query: 414 ESAEDSLLS-SELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 473
             + D LL+ +ELNL +++ A++FH+ S +L+     +   KA++++K+ G L+ +D NL
Sbjct: 131 NPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPCRAAHMKAMEVAKEAGALLSYDPNL 190

Query: 474 LLPLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 533
            LPLW S +E ++ IK  W+ AD+I+VS  ELEFL                         
Sbjct: 191 RLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLTG----------------------S 250

Query: 534 NRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRT 593
           N+ D      E    LWH  L+LL VT G    +YY+  FHG V G         T D T
Sbjct: 251 NKID-----DESAMSLWHPNLKLLLVTLGEKGCNYYTKKFHGTVGG-----FHVKTVDTT 310

Query: 594 GSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNL 653
           G+GD+ V  ++ K+     + E++  L+  LRF+ A G I+    GA+   PT S    L
Sbjct: 311 GAGDSFVGALLTKIVDDQTILEDEARLKEVLRFSCACGAITTTKKGAIPALPTASEALTL 323

BLAST of MC05g1516 vs. ExPASy Swiss-Prot
Match: Q7XJ81 (Fructokinase-2 OS=Solanum habrochaites OX=62890 GN=FRK2 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.4e-47
Identity = 122/360 (33.89%), Postives = 185/360 (51.39%), Query Frame = 0

Query: 294 LICCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLG 353
           LI  FG    +FVP+V                +   + P F +APGGAP+NVAI+  RLG
Sbjct: 11  LIVSFGEMLIDFVPTVS--------------GVSLAEAPGFLKAPGGAPANVAIAVTRLG 70

Query: 354 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVK 413
           GR+AF+GK+G D+FG  L  ++    VQ   + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 71  GRSAFVGKLGDDEFGHMLAGILKTNGVQADGINFDKGARTALAFVTLR-ADGEREFMFYR 130

Query: 414 ESAEDSLLS-SELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 473
             + D LL+ +ELNL +++ A++FH+ S +L+     +   KA++++K+ G L+ +D NL
Sbjct: 131 NPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPCRAAHMKAMEVAKEAGALLSYDPNL 190

Query: 474 LLPLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 533
            LPLW S +E ++ IK  W+ AD+I+VS  ELEFL                         
Sbjct: 191 RLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLTG----------------------S 250

Query: 534 NRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRT 593
           N+ D      E    LWH  L+LL VT G    +YY+  FHG V G         T D T
Sbjct: 251 NKID-----DESAMSLWHPNLKLLLVTLGEKGCNYYTKKFHGTVGG-----FHVKTVDTT 310

Query: 594 GSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNL 653
           G+GD+ V  ++ K+     +  ++  L+  LRF+ A G I+    GA+   PT S    L
Sbjct: 311 GAGDSFVGALLTKIVDDQTILADEARLKEVLRFSCACGAITTTKKGAIPALPTASEALTL 323

BLAST of MC05g1516 vs. ExPASy Swiss-Prot
Match: Q9M1B9 (Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.3e-47
Identity = 123/363 (33.88%), Postives = 184/363 (50.69%), Query Frame = 0

Query: 294 LICCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLG 353
           LI  FG    +FVP+V                +   D P F +APGGAP+NVAI+  RLG
Sbjct: 10  LIVSFGEMLIDFVPTVS--------------GVSLADAPGFIKAPGGAPANVAIAISRLG 69

Query: 354 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVK 413
           GRAAF+GK+G D+FG  L  ++ +  V    + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 70  GRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDTGARTALAFVTLR-SDGEREFMFYR 129

Query: 414 ESAEDSLL-SSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 473
             + D LL   ELNL V++ A++FH+ S +L+     S   KA++++K+ G L+ +D NL
Sbjct: 130 NPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNL 189

Query: 474 LLPLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 533
            LPLW S++E ++ I   W++A++I+VS +EL FL   +  +                  
Sbjct: 190 RLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLTGSDKVD------------------ 249

Query: 534 NRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPF---TC 593
                     E    LWH  L+LL VT G     YY+ SF G V         PF     
Sbjct: 250 ---------DETALSLWHSNLKLLLVTLGEKGCRYYTKSFRGSV--------DPFHVDAV 309

Query: 594 DRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESAT 653
           D TG+GD+ V  ++ K+     + E++  L   LR A A G I+    GA+   PTES  
Sbjct: 310 DTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANACGAITTTKKGAIPALPTESEV 322

BLAST of MC05g1516 vs. NCBI nr
Match: EOY07323.1 (Fructokinase-like 1 [Theobroma cacao])

HSP 1 Score: 961 bits (2484), Expect = 0.0
Identity = 500/668 (74.85%), Postives = 567/668 (84.88%), Query Frame = 0

Query: 1   MGSVFGKISVETPKFEVIQSTADYEIRLYEPSVVAQVTYDPSQFRGNRDGGFTLLAKYIG 60
           MG + GKISVETPK+EV+QS+ADYEIR Y PSVVA+VTYDPSQF+GN+DGGF++LA YIG
Sbjct: 1   MGMILGKISVETPKYEVVQSSADYEIRKYSPSVVAEVTYDPSQFKGNKDGGFSVLANYIG 60

Query: 61  AIGEPQNTKSEKVAMTAPVITKS-----ETIPMTAPVVTGGGGGEGKPVTMQFVLPSKYK 120
           A+G PQN K EK+AMTAPVITKS     E I MTAPV+T G   E   VTMQF+LP+ Y+
Sbjct: 61  ALGNPQNAKPEKIAMTAPVITKSPGSESEKIAMTAPVITKG---EKNMVTMQFLLPAMYE 120

Query: 121 KAEEAPKPADESVVIREEGEKKLAVVKFSGIATEGVVAQKVENLKQSLEKDGVKVIGDFV 180
           +AEEAPKP DE VVIREEGE+K  VVKF+G+AT+ VV +KVENLK+SLE+DG KVIG+F+
Sbjct: 121 RAEEAPKPLDERVVIREEGERKYGVVKFAGVATDQVVEEKVENLKKSLERDGHKVIGEFL 180

Query: 181 LARYNPPWTLPSLRTNEVMIPNSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTK 240
           LARYNPPWTLP+ RTNEVM+P  +    +   RASV  + N  VE+  PTRRGRKK  T 
Sbjct: 181 LARYNPPWTLPAFRTNEVMLPVQT----VGLPRASV--ETNEAVESPKPTRRGRKKKPTS 240

Query: 241 SSSSSTTTQKKTKRSKKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLI 300
           SS ++     KT+R++KT  E+ + E    E   E+E +      +DDGMDFPYE+PPLI
Sbjct: 241 SSPAT-----KTRRTRKTQNENGSIES---ESQGEEEELGD----FDDGMDFPYEEPPLI 300

Query: 301 CCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGR 360
           CCFGAAQKEFVP+VRV++ +MH DKYS WKMLQWDPPEF RAPGG PSNVAISHVRLGGR
Sbjct: 301 CCFGAAQKEFVPTVRVNEEKMHPDKYSHWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGR 360

Query: 361 AAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFED-GKLKMETVKE 420
           AAFMGKVG D++GDELVLMMNKEKVQTR VKFD N +T CTYMK+KF++ G+LKMETVKE
Sbjct: 361 AAFMGKVGDDEYGDELVLMMNKEKVQTRGVKFDENVKTGCTYMKVKFDEKGRLKMETVKE 420

Query: 421 SAEDSLLSSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLL 480
           SAEDSLLSSELNL+VLKE RIFHFNSE L S +M+STLFKAI  SKKFGGLIFFDLNL L
Sbjct: 421 SAEDSLLSSELNLAVLKETRIFHFNSEVLTSPSMQSTLFKAIAWSKKFGGLIFFDLNLPL 480

Query: 481 PLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNR 540
            LWRSRDETRE IK+AWN+ADIIEVSRQELEFLLDEEYYE+KRNYRPQYYA++ +QTKNR
Sbjct: 481 LLWRSRDETREVIKEAWNKADIIEVSRQELEFLLDEEYYERKRNYRPQYYAESFDQTKNR 540

Query: 541 RDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGS 600
           RD YHYT EEISPLWHD L+ LFVTDGT+RIHYY+PSF GVV+GTEDVLITPFTCDRTGS
Sbjct: 541 RDCYHYTSEEISPLWHDGLKYLFVTDGTLRIHYYTPSFDGVVVGTEDVLITPFTCDRTGS 600

Query: 601 GDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKE 660
           GDAVVAGIMRKLTT+PEMFENQD LERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKE
Sbjct: 601 GDAVVAGIMRKLTTYPEMFENQDTLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKE 647

Query: 661 QVYVPSMW 662
           QVYVPSMW
Sbjct: 661 QVYVPSMW 647

BLAST of MC05g1516 vs. NCBI nr
Match: KAF7825590.1 (fructokinase-like 1, chloroplastic [Senna tora])

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 496/678 (73.16%), Postives = 560/678 (82.60%), Query Frame = 0

Query: 1   MGSVFGKISVETPKFEVIQSTADYEIRLYEPSVVAQVTYDPSQFRGNRDGGFTLLAKYIG 60
           MG VFGKISVETPK+EVI+ST+DYEIR Y PSV+AQVTYDPSQF+G++DGGF +LA YIG
Sbjct: 1   MGMVFGKISVETPKYEVIKSTSDYEIRKYPPSVIAQVTYDPSQFKGDKDGGFFILANYIG 60

Query: 61  AIGEPQNTKSEKVAMTAPVITKSETIPMTAPVVTGGGGGEGKPVTMQFVLPSKYKKAEEA 120
           A G+PQN K E++AMTAPVITKSE + MTAPVVT     + K VTM+F+LPSKY KAEEA
Sbjct: 61  AFGKPQNAKPEQIAMTAPVITKSEAVAMTAPVVTRE---KDKRVTMEFILPSKYGKAEEA 120

Query: 121 PKPADESVVIREEGEKKLAVVKFSGIATEGVVAQKVENLKQSLEKDGVKVIGDFVLARYN 180
           PKP DE V IREEGE K  VV+F G+A+E VV +K+E LK+SLE+DG KVIGD+VLARYN
Sbjct: 121 PKPLDERVKIREEGEVKYGVVRFGGVASENVVQEKIEKLKKSLERDGYKVIGDYVLARYN 180

Query: 181 PPWTLPSLRTNEVMIP----NSSKS-----------PRLNPARASVGDDGNGVVETASPT 240
           PPWTLP  RTN+VMIP    N+S             P   P +ASV  D NG +E+ SP 
Sbjct: 181 PPWTLPMFRTNDVMIPVDLRNASSKVLEFQTHFKLKPFHRPVKASVNGD-NGAIESPSPP 240

Query: 241 RRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGNPF-EQVSEDEPIDSIAEKYDDG 300
           RRGRKK   KSSSS +   K++KRS+KT  E+   E  P  E V+E E     AE YDDG
Sbjct: 241 RRGRKK---KSSSSPS---KQSKRSRKTQSENGAMEAKPATETVNEAEITLEDAENYDDG 300

Query: 301 MDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSN 360
           +D PYE PPL+CCFGA QKEFVP+VRVH+  M  D YSEWKMLQW PPEF RAPGG  SN
Sbjct: 301 IDLPYEFPPLVCCFGAVQKEFVPTVRVHEYPMDPDIYSEWKMLQWKPPEFGRAPGGPSSN 360

Query: 361 VAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFED 420
           VAI+HVRLGGR+AF+GKVG DDFGDELVLMMNKE+VQTRAVKFD + +T CTYMK+KFED
Sbjct: 361 VAIAHVRLGGRSAFIGKVGDDDFGDELVLMMNKERVQTRAVKFDSSVKTGCTYMKVKFED 420

Query: 421 GKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGG 480
           GK+KMETVKESAEDSL +SELNLSVLKEAR+FHFNSE L S +M  TLF AI LSKKFGG
Sbjct: 421 GKMKMETVKESAEDSLRTSELNLSVLKEARVFHFNSEVLTSPSMRPTLFHAIALSKKFGG 480

Query: 481 LIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYY 540
           LIFFDLNL LPLW+S DET+E IKKAWNEAD+IEVS+ ELEFLLDEEYYE+KRNYRPQYY
Sbjct: 481 LIFFDLNLPLPLWKSPDETKELIKKAWNEADVIEVSKTELEFLLDEEYYERKRNYRPQYY 540

Query: 541 AQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLI 600
           A+  EQTKNR DYYHYTPEE+SPLWH+ L+ LFVTDGT+RIHYY+P F GVV+GTEDVLI
Sbjct: 541 AENYEQTKNRVDYYHYTPEEVSPLWHNGLKFLFVTDGTLRIHYYTPHFDGVVVGTEDVLI 600

Query: 601 TPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFP 660
           TP+TCDRTGSGDAVVAGI+RKLTT PEMFENQDVLERQLRFAIAAGII+QWTIGAVRGFP
Sbjct: 601 TPYTCDRTGSGDAVVAGIIRKLTTCPEMFENQDVLERQLRFAIAAGIIAQWTIGAVRGFP 660

Query: 661 TESATQNLKEQVYVPSMW 662
           TESATQNLKEQVYVPSMW
Sbjct: 661 TESATQNLKEQVYVPSMW 668

BLAST of MC05g1516 vs. NCBI nr
Match: XP_004302649.2 (PREDICTED: LOW QUALITY PROTEIN: fructokinase-like 1, chloroplastic [Fragaria vesca subsp. vesca])

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 483/665 (72.63%), Postives = 551/665 (82.86%), Query Frame = 0

Query: 1   MGSVFGKISVETPKFEVIQSTADYEIRLYEPSVVAQVTYDPSQFRGNRDGGFTLLAKYIG 60
           MG VFGKI VETPK++V++STADYEIR Y PSV+AQVTYDPS F+G++DGGF++LA YIG
Sbjct: 1   MGMVFGKICVETPKYQVLKSTADYEIRQYPPSVIAQVTYDPSVFKGDKDGGFSVLANYIG 60

Query: 61  AIGEPQNTKSEKVAMTAPVITKSET---IPMTAPVVTGGGGGEGKPVTMQFVLPSKYKKA 120
           A G+PQNTK EK+AMTAPVIT+  T   I MTAPVVT   G + K VTMQF+LP+KY+KA
Sbjct: 61  AFGKPQNTKPEKIAMTAPVITQQSTAEKIAMTAPVVTKEAG-DAKTVTMQFLLPAKYQKA 120

Query: 121 EEAPKPADESVVIREEGEKKLAVVKFSGIATEGVVAQKVENLKQSLEKDGVKVIGDFVLA 180
           EEAPKP DE VVIREEG +K  VV F G A+E VV  +VE LK++LEKDG K+ G+++LA
Sbjct: 121 EEAPKPVDERVVIREEGGRKYGVVTFGGSASEQVVEDRVEKLKRALEKDGFKITGEYLLA 180

Query: 181 RYNPPWTLPSLRTNEVMIPNSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSS 240
           R+NPPWTLP+ RTNEVMIP       LNP RAS G+D   + E   P RRGRKK  T +S
Sbjct: 181 RFNPPWTLPAFRTNEVMIPVEX----LNPLRAS-GEDNGSIEEPKQPARRGRKKSSTSAS 240

Query: 241 SSSTTTQKKTKRSKKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICC 300
           SS     KKT+R+KK  VE+      P E  S+D    S  E YDDG+DFPY+DPPL+CC
Sbjct: 241 SSPP---KKTQRAKKAQVEN-----GPVEPESDD----SELEDYDDGIDFPYDDPPLVCC 300

Query: 301 FGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAA 360
           FGAAQKEFVP+VRV DN MH D YS WKMLQWDPPEF+RAPG +PSNVAISHVRLGGRAA
Sbjct: 301 FGAAQKEFVPTVRVSDNPMHPDMYSRWKMLQWDPPEFSRAPGSSPSNVAISHVRLGGRAA 360

Query: 361 FMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAE 420
           FMGKVG D+FG E++LMMNKE VQTR VK D +++TA TYMKI+F+D ++  ETVKESAE
Sbjct: 361 FMGKVGGDEFGSEVLLMMNKENVQTRGVKVDADAKTARTYMKIRFDDERMWTETVKESAE 420

Query: 421 DSLLSSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLW 480
           DSLL SELNL+VLKEAR+FHF SE L S  M+  L++AI  +KKFGGL+FFDLNL LPLW
Sbjct: 421 DSLLRSELNLAVLKEARMFHFTSEVLTSAPMQPALYRAIAYAKKFGGLVFFDLNLPLPLW 480

Query: 481 RSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDY 540
           +SRDETRE+IKKAW EAD+IEVSRQELEFLLDEEYY +KRNYRPQYYAQ  EQTK RRDY
Sbjct: 481 KSRDETREFIKKAWYEADVIEVSRQELEFLLDEEYYVRKRNYRPQYYAQEYEQTKKRRDY 540

Query: 541 YHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDA 600
           YHYTPEEI+PLWHD+L+ LFVTDGT+RIHYYSPSF GVVIGTEDVLITPFTCDRTGSGDA
Sbjct: 541 YHYTPEEIAPLWHDKLKFLFVTDGTLRIHYYSPSFDGVVIGTEDVLITPFTCDRTGSGDA 600

Query: 601 VVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVY 660
           VVA IMRKLTTFPEM+ENQDV ERQLRFA+AAGIISQWTIGAVRGFPTESATQNLKEQVY
Sbjct: 601 VVAAIMRKLTTFPEMYENQDVCERQLRFAVAAGIISQWTIGAVRGFPTESATQNLKEQVY 647

Query: 661 VPSMW 662
           VPSMW
Sbjct: 661 VPSMW 647

BLAST of MC05g1516 vs. NCBI nr
Match: XP_022145754.1 (fructokinase-like 1, chloroplastic [Momordica charantia])

HSP 1 Score: 921 bits (2380), Expect = 0.0
Identity = 466/472 (98.73%), Postives = 467/472 (98.94%), Query Frame = 0

Query: 191 NEVMIPNSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRS 250
           N   + NSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRS
Sbjct: 46  NSRYVQNSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRS 105

Query: 251 KKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVR 310
           KKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVR
Sbjct: 106 KKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVR 165

Query: 311 VHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDE 370
           VHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDE
Sbjct: 166 VHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDE 225

Query: 371 LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVL 430
           LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVL
Sbjct: 226 LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVL 285

Query: 431 KEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKA 490
           KEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKA
Sbjct: 286 KEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKA 345

Query: 491 WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWH 550
           WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWH
Sbjct: 346 WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWH 405

Query: 551 DRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP 610
           DRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP
Sbjct: 406 DRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP 465

Query: 611 EMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 662
           EMFENQD LERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 466 EMFENQDXLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

BLAST of MC05g1516 vs. NCBI nr
Match: XP_038906230.1 (fructokinase-like 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 837 bits (2162), Expect = 1.74e-300
Identity = 421/466 (90.34%), Postives = 440/466 (94.42%), Query Frame = 0

Query: 197 NSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVE 256
           +SS+S + N  RASV  DGNGVVET SPTRRGRKKG T  SSSST  QKKTKRSKK  + 
Sbjct: 47  SSSQSLKQNTPRASVNGDGNGVVETPSPTRRGRKKGTTSPSSSST--QKKTKRSKKPQIT 106

Query: 257 DETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQM 316
           DETTEGNPF+  SEDEPIDSIA+ YDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQM
Sbjct: 107 DETTEGNPFDHASEDEPIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQM 166

Query: 317 HADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMN 376
           H DKYSEWKMLQWDPPEFARAPGG+PSNVAISHVRLGGRAAFMGKVGKDDFGDE+VLMMN
Sbjct: 167 HPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDEVVLMMN 226

Query: 377 KEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIF 436
           KEKVQTRAVKFDLNSRTACTYMKIKFEDG+LKMETVKE AEDSL+SSELNL+VLKEARIF
Sbjct: 227 KEKVQTRAVKFDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIF 286

Query: 437 HFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADI 496
           HFNSEAL+STAM+STLFKAIQLSKKFGGLIFFDLNL LPLW+SRDETREYIKKAWNEADI
Sbjct: 287 HFNSEALLSTAMKSTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADI 346

Query: 497 IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLL 556
           IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEISPLWHDRL+LL
Sbjct: 347 IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLL 406

Query: 557 FVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQ 616
           FVTDGT+RIHYYSPSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPE+  NQ
Sbjct: 407 FVTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEILVNQ 466

Query: 617 DVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 662
           D LERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 467 DALERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 510

BLAST of MC05g1516 vs. ExPASy TrEMBL
Match: A0A061ERN1 (Fructokinase-like 1 OS=Theobroma cacao OX=3641 GN=TCM_021784 PE=3 SV=1)

HSP 1 Score: 961 bits (2484), Expect = 0.0
Identity = 500/668 (74.85%), Postives = 567/668 (84.88%), Query Frame = 0

Query: 1   MGSVFGKISVETPKFEVIQSTADYEIRLYEPSVVAQVTYDPSQFRGNRDGGFTLLAKYIG 60
           MG + GKISVETPK+EV+QS+ADYEIR Y PSVVA+VTYDPSQF+GN+DGGF++LA YIG
Sbjct: 1   MGMILGKISVETPKYEVVQSSADYEIRKYSPSVVAEVTYDPSQFKGNKDGGFSVLANYIG 60

Query: 61  AIGEPQNTKSEKVAMTAPVITKS-----ETIPMTAPVVTGGGGGEGKPVTMQFVLPSKYK 120
           A+G PQN K EK+AMTAPVITKS     E I MTAPV+T G   E   VTMQF+LP+ Y+
Sbjct: 61  ALGNPQNAKPEKIAMTAPVITKSPGSESEKIAMTAPVITKG---EKNMVTMQFLLPAMYE 120

Query: 121 KAEEAPKPADESVVIREEGEKKLAVVKFSGIATEGVVAQKVENLKQSLEKDGVKVIGDFV 180
           +AEEAPKP DE VVIREEGE+K  VVKF+G+AT+ VV +KVENLK+SLE+DG KVIG+F+
Sbjct: 121 RAEEAPKPLDERVVIREEGERKYGVVKFAGVATDQVVEEKVENLKKSLERDGHKVIGEFL 180

Query: 181 LARYNPPWTLPSLRTNEVMIPNSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTK 240
           LARYNPPWTLP+ RTNEVM+P  +    +   RASV  + N  VE+  PTRRGRKK  T 
Sbjct: 181 LARYNPPWTLPAFRTNEVMLPVQT----VGLPRASV--ETNEAVESPKPTRRGRKKKPTS 240

Query: 241 SSSSSTTTQKKTKRSKKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLI 300
           SS ++     KT+R++KT  E+ + E    E   E+E +      +DDGMDFPYE+PPLI
Sbjct: 241 SSPAT-----KTRRTRKTQNENGSIES---ESQGEEEELGD----FDDGMDFPYEEPPLI 300

Query: 301 CCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGR 360
           CCFGAAQKEFVP+VRV++ +MH DKYS WKMLQWDPPEF RAPGG PSNVAISHVRLGGR
Sbjct: 301 CCFGAAQKEFVPTVRVNEEKMHPDKYSHWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGR 360

Query: 361 AAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFED-GKLKMETVKE 420
           AAFMGKVG D++GDELVLMMNKEKVQTR VKFD N +T CTYMK+KF++ G+LKMETVKE
Sbjct: 361 AAFMGKVGDDEYGDELVLMMNKEKVQTRGVKFDENVKTGCTYMKVKFDEKGRLKMETVKE 420

Query: 421 SAEDSLLSSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLL 480
           SAEDSLLSSELNL+VLKE RIFHFNSE L S +M+STLFKAI  SKKFGGLIFFDLNL L
Sbjct: 421 SAEDSLLSSELNLAVLKETRIFHFNSEVLTSPSMQSTLFKAIAWSKKFGGLIFFDLNLPL 480

Query: 481 PLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNR 540
            LWRSRDETRE IK+AWN+ADIIEVSRQELEFLLDEEYYE+KRNYRPQYYA++ +QTKNR
Sbjct: 481 LLWRSRDETREVIKEAWNKADIIEVSRQELEFLLDEEYYERKRNYRPQYYAESFDQTKNR 540

Query: 541 RDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGS 600
           RD YHYT EEISPLWHD L+ LFVTDGT+RIHYY+PSF GVV+GTEDVLITPFTCDRTGS
Sbjct: 541 RDCYHYTSEEISPLWHDGLKYLFVTDGTLRIHYYTPSFDGVVVGTEDVLITPFTCDRTGS 600

Query: 601 GDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKE 660
           GDAVVAGIMRKLTT+PEMFENQD LERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKE
Sbjct: 601 GDAVVAGIMRKLTTYPEMFENQDTLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKE 647

Query: 661 QVYVPSMW 662
           QVYVPSMW
Sbjct: 661 QVYVPSMW 647

BLAST of MC05g1516 vs. ExPASy TrEMBL
Match: A0A6J1CW73 (fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015134 PE=4 SV=1)

HSP 1 Score: 921 bits (2380), Expect = 0.0
Identity = 466/472 (98.73%), Postives = 467/472 (98.94%), Query Frame = 0

Query: 191 NEVMIPNSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRS 250
           N   + NSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRS
Sbjct: 46  NSRYVQNSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRS 105

Query: 251 KKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVR 310
           KKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVR
Sbjct: 106 KKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVR 165

Query: 311 VHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDE 370
           VHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDE
Sbjct: 166 VHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDE 225

Query: 371 LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVL 430
           LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVL
Sbjct: 226 LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVL 285

Query: 431 KEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKA 490
           KEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKA
Sbjct: 286 KEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKA 345

Query: 491 WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWH 550
           WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWH
Sbjct: 346 WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWH 405

Query: 551 DRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP 610
           DRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP
Sbjct: 406 DRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP 465

Query: 611 EMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 662
           EMFENQD LERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 466 EMFENQDXLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

BLAST of MC05g1516 vs. ExPASy TrEMBL
Match: F6HKY0 (PfkB domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_08s0007g01640 PE=3 SV=1)

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 482/747 (64.52%), Postives = 552/747 (73.90%), Query Frame = 0

Query: 1   MGSVFGKISVETPKFEVIQSTADYEIRLYEPSVVAQVTYDPSQFRGNRDGGFTLLAKYIG 60
           MG VFGKI VETPKF+VIQS+ADYEIR Y P+V+A+VTYDPSQFRG++DGGFTLLA YIG
Sbjct: 1   MGMVFGKICVETPKFQVIQSSADYEIRKYPPTVIAEVTYDPSQFRGDKDGGFTLLANYIG 60

Query: 61  AIGEPQNTKSEKVAMTAPVITK-SETIPMTAPVVTGGG-GGEGKPVTMQFVLPSKYKKAE 120
           A+G PQNTK EK+ MTAPV+TK +E I MTAPVVT  G GGEGK VTMQF+LPSKY KAE
Sbjct: 61  ALGNPQNTKPEKIEMTAPVVTKYAEKIAMTAPVVTKSGEGGEGKTVTMQFLLPSKYTKAE 120

Query: 121 EAPKPADESVVIREEGEKKLAVVKFSGIATEGVVAQKVENLKQSLEKDGVKVIGDFVLAR 180
           EAP+P DE VVIREEGE+K  VVKF G+ATE VV  KVE+L++SLE+DG K+IG+FVLAR
Sbjct: 121 EAPRPVDERVVIREEGERKYGVVKFGGVATEKVVGAKVESLEKSLERDGFKLIGEFVLAR 180

Query: 181 YNPPWTLPSLRTNEVMIPNSSKSPRL---------------------------------- 240
           YNPPWTLP+ RTNEVMIP    SP L                                  
Sbjct: 181 YNPPWTLPAFRTNEVMIPIELVSPILFSLFSVKVSYYVPLSFCTLMQKADSFDAHQCLCC 240

Query: 241 -------------------------------------------------NPARASVGDDG 300
                                                             P RA++    
Sbjct: 241 RTHTSEKYIQTMAAFHLLPPPQRSYQILFNPIKLTCLSSTFHLQKPQNPKPLRAAISTQ- 300

Query: 301 NGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGNPFEQVSEDEPID 360
           +G ++T  PTRRGR+KG T SSSS TT  K++ +  +T  E E T+  P E         
Sbjct: 301 DGALDTTKPTRRGRQKGTTTSSSSQTTRPKRSPKKNQTEAEVEITKQEPAEH-------- 360

Query: 361 SIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFA 420
             ++  +D +DFPY+DPPLICCFGAAQKEFVP+VRV ++QMH D YSEWKMLQWDPPEF 
Sbjct: 361 --SDDNNDEIDFPYDDPPLICCFGAAQKEFVPTVRVSEHQMHPDIYSEWKMLQWDPPEFV 420

Query: 421 RAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTAC 480
           RAPGG PSNVAISHVRLGGRAAFMGKVG+D+FG+ELVL MNKEKVQTRAVKFD N+RT C
Sbjct: 421 RAPGGPPSNVAISHVRLGGRAAFMGKVGRDEFGEELVLTMNKEKVQTRAVKFDSNARTGC 480

Query: 481 TYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEALMSTAMESTLFKA 540
           +YM+IKFE+GK+ METVKES EDSLLSSELN++VLKEARIFHFNSE L S +M S+LF A
Sbjct: 481 SYMRIKFENGKMMMETVKESPEDSLLSSELNIAVLKEARIFHFNSEVLTSPSMHSSLFGA 540

Query: 541 IQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEK 600
           I+LSKK GGL+FFDLNL LPLWRSRDETR+ I+KAWN+ADIIE+                
Sbjct: 541 IELSKKHGGLVFFDLNLPLPLWRSRDETRKLIEKAWNQADIIEM---------------- 600

Query: 601 KRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGV 660
           +RNYRPQYY+ + EQTKNRRDYYHYT +EISPLWH+ L+ LFVTDGT+R+HYYSPSF GV
Sbjct: 601 RRNYRPQYYSDSYEQTKNRRDYYHYTRDEISPLWHEGLKFLFVTDGTLRVHYYSPSFDGV 660

Query: 661 VIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQW 662
           V+GTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEM+ENQD LERQLRFAIAAGIISQW
Sbjct: 661 VVGTEDVLITPFTCDRTGSGDAIVAGIMRKLTTFPEMYENQDTLERQLRFAIAAGIISQW 720

BLAST of MC05g1516 vs. ExPASy TrEMBL
Match: A0A5A7TRC7 (Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001140 PE=4 SV=1)

HSP 1 Score: 829 bits (2142), Expect = 9.64e-298
Identity = 417/466 (89.48%), Postives = 439/466 (94.21%), Query Frame = 0

Query: 197 NSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVE 256
           +SSK+ +LNP RAS   +GNGVVET SPTRRGRKKG T S SSST+ QKKTKRSKK  + 
Sbjct: 47  SSSKTLKLNPPRASTNGEGNGVVETPSPTRRGRKKG-TASPSSSTSAQKKTKRSKKPQIT 106

Query: 257 DETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQM 316
           D+TT  NPF+ +SEDE IDSIA+ YDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQM
Sbjct: 107 DKTTVANPFDHISEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQM 166

Query: 317 HADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMN 376
            ADKYSEWKMLQWDPPEFARAPGG+PSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMN
Sbjct: 167 DADKYSEWKMLQWDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMN 226

Query: 377 KEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIF 436
           KEKVQTRAVKFDLNS+TACTYMKIKFEDGKLKMETVKE AEDSLLSSELNL+VLKEARIF
Sbjct: 227 KEKVQTRAVKFDLNSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIF 286

Query: 437 HFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADI 496
           HFNSEAL+ST +E TLFKAIQLSKKFGGLIFFDLNL LPLW+SR+ETREYIKKAWNEADI
Sbjct: 287 HFNSEALLSTVIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADI 346

Query: 497 IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLL 556
           IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEISPLWHD L+LL
Sbjct: 347 IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLL 406

Query: 557 FVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQ 616
           FVTDGT+RIHYYSPSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQ
Sbjct: 407 FVTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQ 466

Query: 617 DVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 662
           DVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 467 DVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of MC05g1516 vs. ExPASy TrEMBL
Match: A0A1S4DUM6 (fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 SV=1)

HSP 1 Score: 829 bits (2142), Expect = 9.64e-298
Identity = 417/466 (89.48%), Postives = 439/466 (94.21%), Query Frame = 0

Query: 197 NSSKSPRLNPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVE 256
           +SSK+ +LNP RAS   +GNGVVET SPTRRGRKKG T S SSST+ QKKTKRSKK  + 
Sbjct: 47  SSSKTLKLNPPRASTNGEGNGVVETPSPTRRGRKKG-TASPSSSTSAQKKTKRSKKPQIT 106

Query: 257 DETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQM 316
           D+TT  NPF+ +SEDE IDSIA+ YDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQM
Sbjct: 107 DKTTVANPFDHISEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQM 166

Query: 317 HADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMN 376
            ADKYSEWKMLQWDPPEFARAPGG+PSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMN
Sbjct: 167 DADKYSEWKMLQWDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMN 226

Query: 377 KEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIF 436
           KEKVQTRAVKFDLNS+TACTYMKIKFEDGKLKMETVKE AEDSLLSSELNL+VLKEARIF
Sbjct: 227 KEKVQTRAVKFDLNSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIF 286

Query: 437 HFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADI 496
           HFNSEAL+ST +E TLFKAIQLSKKFGGLIFFDLNL LPLW+SR+ETREYIKKAWNEADI
Sbjct: 287 HFNSEALLSTVIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADI 346

Query: 497 IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLL 556
           IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEISPLWHD L+LL
Sbjct: 347 IEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLL 406

Query: 557 FVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQ 616
           FVTDGT+RIHYYSPSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQ
Sbjct: 407 FVTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQ 466

Query: 617 DVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 662
           DVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 467 DVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of MC05g1516 vs. TAIR 10
Match: AT3G54090.1 (fructokinase-like 1 )

HSP 1 Score: 674.1 bits (1738), Expect = 1.2e-193
Identity = 343/482 (71.16%), Postives = 393/482 (81.54%), Query Frame = 0

Query: 187 SLRTNEVMIPNSSKSPR--LNPARASVGDDG--NGVV--ETASPTRRGRKKGMTKSSSSS 246
           SL   EV++   S++ R  L P +AS+   G  NG     T+ P+R+GRKK  T      
Sbjct: 16  SLDRREVLVVRHSQASRRFLTP-KASINGSGITNGAAAETTSKPSRKGRKKKQT------ 75

Query: 247 TTTQKKTKRSKKTLVEDETTEGNPFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGA 306
                       T++E + TE +P         ++     YDDG++FPY+DPPL+CCFGA
Sbjct: 76  -----------STVIEKDNTETDP--------ELNPELADYDDGIEFPYDDPPLVCCFGA 135

Query: 307 AQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMG 366
            QKEFVP VRVHDN MH D YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMG
Sbjct: 136 VQKEFVPVVRVHDNPMHPDMYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMG 195

Query: 367 KVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSL 426
           KVG+DDFGDELVLMMN+E+VQTRAVKFD NS+TACT +KIKF+DGK+  ETVKE  EDSL
Sbjct: 196 KVGEDDFGDELVLMMNQERVQTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSL 255

Query: 427 LSSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSR 486
            +SELNL+VLKEARIFHFNSE L S  M+STLF AIQ SKKFGGLIFFDLNL LPLWRSR
Sbjct: 256 FASELNLAVLKEARIFHFNSEVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSR 315

Query: 487 DETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHY 546
           +ETR+ IKKAWNEA+IIEVS+QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRDYYHY
Sbjct: 316 NETRKLIKKAWNEANIIEVSQQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHY 375

Query: 547 TPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVA 606
           TPEEI  LWHD+L+LL VTDGT+R+HYY+P+F GVVIGTEDVLITPFTCDRTGSGDAVVA
Sbjct: 376 TPEEIKSLWHDKLKLLVVTDGTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVA 435

Query: 607 GIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPS 663
           GIMRKLTT PEMFE+QDV+ERQLRFA+AAGII+QWTIGAVRGFPTESATQNLKEQVYVPS
Sbjct: 436 GIMRKLTTCPEMFEDQDVMERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPS 471

BLAST of MC05g1516 vs. TAIR 10
Match: AT1G69200.1 (fructokinase-like 2 )

HSP 1 Score: 325.5 bits (833), Expect = 1.0e-88
Identity = 184/451 (40.80%), Postives = 269/451 (59.65%), Query Frame = 0

Query: 214 DGNGVVETASPTR-RGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGN--------- 273
           D   +V    P + R RKK    SS       +K  R K+T+ +D+  E +         
Sbjct: 109 DKESIVSALKPKKTRTRKKAAAASSDVEEVKTEKKVRRKRTVKKDKDVEDDLATIMDAEV 168

Query: 274 ----------PFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHD 333
                       +  SE+E ID +++   + +   Y  PPL+CCFG+AQ  FVPS R  +
Sbjct: 169 SDVEEALAVESTDTESEEEEID-LSKHEGEDISHTYGWPPLVCCFGSAQHAFVPSGRPAN 228

Query: 334 NQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVL 393
             +  + +   +  +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++ 
Sbjct: 229 RLLDYELHERMRDAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAMLY 288

Query: 394 MMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEA 453
            +N  KVQTR+VK D    TAC+ MKI  + G+LK   +K  AEDSL  SE+N+ VLKEA
Sbjct: 289 YLNVCKVQTRSVKIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKEA 348

Query: 454 RIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNE 513
           ++F+F++ +L+   M ST  +AI++SK+ G +IF+DLNL LPLW S +ET+ +I++ WN 
Sbjct: 349 KMFYFSTHSLLDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNL 408

Query: 514 ADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRL 573
           AD+IE+++QELEFL   E  E       ++  +  + +K    + HY PE +  LWH+ L
Sbjct: 409 ADVIEITKQELEFLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHENL 468

Query: 574 RLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMF 633
           ++LFVT+GT +IHYY+   +G V G EDV ITPFT D + SGD +VAG++R LT  P++ 
Sbjct: 469 KVLFVTNGTSKIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLM 528

Query: 634 ENQDVLERQLRFAIAAGIISQWTIGAVRGFP 645
            N+  LER  R+AI  GII QW +   RG+P
Sbjct: 529 NNKGYLERTARYAIECGIIDQWLLAQTRGYP 546

BLAST of MC05g1516 vs. TAIR 10
Match: AT2G37970.1 (SOUL heme-binding family protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.8e-70
Identity = 138/213 (64.79%), Postives = 161/213 (75.59%), Query Frame = 0

Query: 1   MGSVFGKISVETPKFEVIQSTADYEIRLYEPSVVAQVTYDPSQFRGNRDGGFTLLAKYIG 60
           MG VFGKI+VETPK+ V +S   YEIR Y P+V A+VTYD S+F+G++DGGF LLAKYIG
Sbjct: 11  MGMVFGKIAVETPKYTVTKSGDGYEIREYPPAVAAEVTYDASEFKGDKDGGFQLLAKYIG 70

Query: 61  AIGEPQNTKSEKVAMTAPVITK---------------SETIPMTAPVVTGGGGGEG--KP 120
             G+P+N K EK+AMTAPVITK               SE I MT+PVVT  GGGEG  K 
Sbjct: 71  VFGKPENEKPEKIAMTAPVITKEGEKIAMTAPVITKESEKIEMTSPVVTKEGGGEGRKKL 130

Query: 121 VTMQFVLPSKYKKAEEAPKPADESVVIREEGEKKLAVVKFSGIATEGVVAQKVENLKQSL 180
           VTMQF+LPS YKKAEEAP+P DE VVI+EEG +K  V+KFSGIA+E VV++KV+ L   L
Sbjct: 131 VTMQFLLPSMYKKAEEAPRPTDERVVIKEEGGRKYGVIKFSGIASESVVSEKVKKLSSHL 190

Query: 181 EKDGVKVIGDFVLARYNPPWTLPSLRTNEVMIP 197
           EKDG K+ GDFVLARYNPPWTLP  RTNEVMIP
Sbjct: 191 EKDGFKITGDFVLARYNPPWTLPPFRTNEVMIP 223

BLAST of MC05g1516 vs. TAIR 10
Match: AT3G59480.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 189.9 bits (481), Expect = 6.6e-48
Identity = 123/363 (33.88%), Postives = 184/363 (50.69%), Query Frame = 0

Query: 294 LICCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLG 353
           LI  FG    +FVP+V                +   D P F +APGGAP+NVAI+  RLG
Sbjct: 10  LIVSFGEMLIDFVPTVS--------------GVSLADAPGFIKAPGGAPANVAIAISRLG 69

Query: 354 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVK 413
           GRAAF+GK+G D+FG  L  ++ +  V    + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 70  GRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDTGARTALAFVTLR-SDGEREFMFYR 129

Query: 414 ESAEDSLL-SSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 473
             + D LL   ELNL V++ A++FH+ S +L+     S   KA++++K+ G L+ +D NL
Sbjct: 130 NPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNL 189

Query: 474 LLPLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 533
            LPLW S++E ++ I   W++A++I+VS +EL FL   +  +                  
Sbjct: 190 RLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLTGSDKVD------------------ 249

Query: 534 NRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPF---TC 593
                     E    LWH  L+LL VT G     YY+ SF G V         PF     
Sbjct: 250 ---------DETALSLWHSNLKLLLVTLGEKGCRYYTKSFRGSV--------DPFHVDAV 309

Query: 594 DRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESAT 653
           D TG+GD+ V  ++ K+     + E++  L   LR A A G I+    GA+   PTES  
Sbjct: 310 DTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANACGAITTTKKGAIPALPTESEV 322

BLAST of MC05g1516 vs. TAIR 10
Match: AT1G06020.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 188.0 bits (476), Expect = 2.5e-47
Identity = 124/364 (34.07%), Postives = 188/364 (51.65%), Query Frame = 0

Query: 294 LICCFGAAQKEFVPSVRVHDNQMHADKYSEWKMLQWDPPEFARAPGGAPSNVAISHVRLG 353
           LI  FG    +FVP+V                +   + P F +APGGAP+NVAI+  RLG
Sbjct: 10  LIVSFGEMLIDFVPTVS--------------GVSLSESPGFLKAPGGAPANVAIAVSRLG 69

Query: 354 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKLKMETVK 413
           GRAAF+GK+G DDFG  L  ++ K  V  + + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 70  GRAAFVGKLGDDDFGHMLAGILRKNGVDDQGINFDEGARTALAFVTLR-SDGEREFMFYR 129

Query: 414 ESAEDSLL-SSELNLSVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 473
             + D LL   ELNL +++ A++FH+ S +L++    S   KA++++K+ G L+ +D NL
Sbjct: 130 NPSADMLLRPDELNLELIRSAKVFHYGSISLITEPCRSAHMKAMEVAKEAGALLSYDPNL 189

Query: 474 LLPLWRSRDETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 533
             PLW S +E R  I   W++ADII+VS  ELEFL + +  + K                
Sbjct: 190 REPLWPSPEEARTQIMSIWDKADIIKVSDVELEFLTENKTMDDK---------------- 249

Query: 534 NRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRT 593
                          LWH  L+LL VT G     Y++  FHG V   E   +     D T
Sbjct: 250 -----------TAMSLWHPNLKLLLVTLGEKGCTYFTKKFHGSV---ETFHVD--AVDTT 309

Query: 594 GSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTE-SATQN 653
           G+GD+ V  +++++     + E++  L + LRFA A G I+    GA+   PT+  A   
Sbjct: 310 GAGDSFVGALLQQIVDDQSVLEDEARLRKVLRFANACGAITTTKKGAIPALPTDIEALSF 326

Query: 654 LKEQ 656
           LK+Q
Sbjct: 370 LKDQ 326