MC05g1504 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1504 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 1.0e-167
Identity = 320/484 (66.12%), Postives = 377/484 (77.89%), Query Frame = 0

Query: 111 DEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG-WPLNQTVGEEGGGDKLHFDDRE 170
           +EK  +    SS ++     E + S  E+ ++S +   W +    GE+    K+  D R 
Sbjct: 12  EEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEK---KKIRSDSR- 71

Query: 171 LEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEP 230
                  VSE+E+M ERFSKLLLGEDMSG GNGV TALAISNAITNLCATLFGQLWRLEP
Sbjct: 72  -------VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEP 131

Query: 231 LAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKL 290
           L  E+K MWRREMEWLLCVS+HIVE+ P WQTFPDG+KLEIMTCRPRSDLY NLPALRKL
Sbjct: 132 LPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKL 191

Query: 291 DHMLLDILDSFVDSEFWYVDQGILAAE--ADG-SSFRKLLERQEDKWWLPVPRVPSGGLS 350
           D+MLL+ILDSF ++EFWYVDQGI+A E  ADG SSFRK  +RQEDKWWLPVPRV  GGL 
Sbjct: 192 DNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQ 251

Query: 351 EASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISS 410
           E SR+QLQHKRDCTNQILKAAMAINS+TLADM++P+SYLE+LP+ GR+ LG+ IYR+ISS
Sbjct: 252 ENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISS 311

Query: 411 DEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKE 470
           D+FSP CLL+CLD+SSEHQAIEIANRVE+++Y W K   SKP  N+     K+SWEM+KE
Sbjct: 312 DQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNT-----KTSWEMVKE 371

Query: 471 LVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESL 530
           L++DADK EL A+RAES+L  LKQ+FP LPQT LDMSKIQ+NKD+GK+ILESYSRVLESL
Sbjct: 372 LMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESL 431

Query: 531 AFNIVARIDDLLYVDDLAKH-SDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKP 589
           AFNIVARIDDLL+VDDL +H SD+IP  + LG    N  N    S+    S YTT    P
Sbjct: 432 AFNIVARIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTT----P 469

BLAST of MC05g1504 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 5.2e-151
Identity = 298/596 (50.00%), Postives = 400/596 (67.11%), Query Frame = 0

Query: 86  MEIRVEDKEGKEEK-CDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSST 145
           ME  V+   G E+K  ++  +   +  E + +  S S +S +  ++    S       S 
Sbjct: 1   MENLVKSCAGIEKKRSNLTPSMEDILTESK-ERSSTSGASYESSSTTTVASSSPPPPPSQ 60

Query: 146 LMGWPLNQTVGEEGGGDKLHFDDREL---------EREASSVSEMEIMERFSKLLLGEDM 205
           ++GWP+ +    +   + ++FD ++L          +E +S     + ERF+KLLLGEDM
Sbjct: 61  ILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDM 120

Query: 206 SGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELI 265
           SG G GV TALAISNAITNLCAT+FGQLWRLEPL++E+K MWRREMEW+L VS+HIVEL 
Sbjct: 121 SGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELT 180

Query: 266 PVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAE 325
           P  QT+PDG+K E+MTCRPR DL+ NLPALRKLD+MLLDIL SF  +EFWYVDQGI+A+E
Sbjct: 181 PSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASE 240

Query: 326 ADGS-SFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTL 385
            DGS SFR+ ++RQE+KWWLPVPR+   GL+E +R +L HKR+C  QILKAAMAINS+ L
Sbjct: 241 NDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLAL 300

Query: 386 ADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEA 445
            +MDVP SYLE LPKNGR+ LG+ IYR+++SD+FS   LL+CLD+SSEH A++IANRVEA
Sbjct: 301 TEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEA 360

Query: 446 AMYAWRKGMGSK--PTNNSLRSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFP 505
           ++Y WR+ + +K    NN+  ++ K +WEM+KEL+   DK  L  ER+E++L CLKQ+FP
Sbjct: 361 SIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFP 420

Query: 506 NLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRI 565
           +L QT+LD+SKIQ NKD+GK+ILESYSR LESLA NI+ARIDDLLYVDDL K SD+   +
Sbjct: 421 SLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLL 480

Query: 566 SQ---LGIVAHNTSNRIQFSVPFSSSPYTTTF-TKPSFSPIPIISQ-------------- 625
           S      I+AH     + + +  S +PY T+F T P FSP  I  +              
Sbjct: 481 SSPAVSSIIAHKKVVPLPYLISASGTPYRTSFSTTPGFSPSMISPKKGERRTPYSSKDTN 540

Query: 626 ------TPHRGIGVN---NESIDNVASMKGCGNFIEKLD--LVAEIAEDEGSERSK 640
                  P RG GV    N  +   + +K C N  +  D  ++ +I++D   E+ +
Sbjct: 541 KIIEKGLPSRGYGVRRVLNNYLGMESKLKICVNPSDNADTAVINQISKDVEEEKKR 595

BLAST of MC05g1504 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.1e-131
Identity = 271/510 (53.14%), Postives = 358/510 (70.20%), Query Frame = 0

Query: 119 SASSSSSDLLTSEATGSHDEESSS-----STLMGWPLNQTVGEEGGG-----DKLHFDDR 178
           S SSSSS + +S    S  E S S     S ++GWPL Q    +  G          DD 
Sbjct: 37  SLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDF 96

Query: 179 ELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLE 238
             +R  +  SE+E++ ER +KLLLGEDMSG G GV  ALAISNAITNL A + GQ WRLE
Sbjct: 97  GFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLE 156

Query: 239 PLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRK 298
           P+ +E+K MWRRE+E LL VS+HIVEL+P +Q FP+G+K+E+M CRPRSDL+  LPALRK
Sbjct: 157 PIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRK 216

Query: 299 LDHMLLDILDSFVDSEFWYVDQGILAAE-ADGSSFRKLLERQEDKWWLPVPRVPSGGLSE 358
           LD+ML++ILDSF ++EFWYVDQGI+AAE A  +SFR+      DKWWLP+PRVPS GL+E
Sbjct: 217 LDNMLIEILDSFGETEFWYVDQGIVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTE 276

Query: 359 ASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSD 418
            +R++L H R+ TNQILKA M+INS+ LA+M+VP SYLEALPKNGR+ LG+ +YR+I+SD
Sbjct: 277 QTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSD 336

Query: 419 EFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKEL 478
            FS   LLE +D+SSE   +E+ANRVEA+MY WR+   S+   +  RS++     ++KE+
Sbjct: 337 NFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEM 396

Query: 479 VI---DADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 538
           ++   D DK E+FAERAES+L  LKQ+FP L QT LD SKIQ+NKDVGK+ILESYSRVLE
Sbjct: 397 MMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLE 456

Query: 539 SLAFNIVARIDDLLYVDDLAKH---------SDEIPRISQ------LGIVAHNTSNRIQF 594
           SLA++I  RI+++L++DD++K          SD++  +S+       G +    S    F
Sbjct: 457 SLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLF 516

BLAST of MC05g1504 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 467.6 bits (1202), Expect = 2.3e-130
Identity = 262/515 (50.87%), Postives = 349/515 (67.77%), Query Frame = 0

Query: 91  EDKEGKEEKCDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG--- 150
           ED E   E+C  +  +  +        ES SSSS      +  G+     SS  ++    
Sbjct: 8   EDDEVSSERCGSYSPSADI-------SESESSSSFSCHRFDGEGASSSIPSSPRVVAGRG 67

Query: 151 --WPLNQTVGEEGGGDKLHFDDRELEREASSVSEMEIMERFSKLLLGEDMSGCGNGVSTA 210
             +P    +   GG D + +DD++ + + S +  M+  ERF+KLLLGEDMSG G GV TA
Sbjct: 68  FYFPAPVMLPVIGGKDVV-WDDKQPDNDLSEIEMMK--ERFAKLLLGEDMSGGGKGVCTA 127

Query: 211 LAISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGS 270
           LAISNAITNL AT+FG+LWRLEPLA ++K MWRRE+EWLLCVS+ IVELIP  Q FP G 
Sbjct: 128 LAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGG 187

Query: 271 KLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLL 330
             EIM  RPRSDLYANLPAL+KLD ML+D+LD+F D+EFWY D+GI+  + D  S+    
Sbjct: 188 TYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPA 247

Query: 331 E-RQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYL 390
             RQEDKWWLP P+VP  GLSE +R++LQ  RD  NQILKAA+AINS  LA+M++PD YL
Sbjct: 248 SVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYL 307

Query: 391 EALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMG 450
           E LPK+G+  LGE IY+++++++FSP CLL+CLD+SSEHQ +EIANR+EAA++ WR+  G
Sbjct: 308 ETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNG 367

Query: 451 SKPTNNSLRSSAKSSW-EMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSK 510
            +  +        SSW   +K LV D ++++   +RAE++L  L+ +FP LPQT LDM+K
Sbjct: 368 RR--HKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNK 427

Query: 511 IQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTS 570
           IQ+NKDVG++ILESYSRV+ES+AFNI ARIDD+LYVDD  + S  I     L + + N  
Sbjct: 428 IQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRS--ISVTESLSLFSINGL 487

Query: 571 NRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHR 599
           N  Q +    SSP+ + F  P+ S   + S++P R
Sbjct: 488 NP-QKAFSVQSSPHGSPFATPALS---VASRSPRR 504

BLAST of MC05g1504 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 4.9e-117
Identity = 217/381 (56.96%), Postives = 293/381 (76.90%), Query Frame = 0

Query: 176 SVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERK 235
           S  E+E M ERF+KLLLGEDMSG G GV TA+ ISNAITNL AT+FGQ  RLEPL  E++
Sbjct: 101 SEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKR 160

Query: 236 GMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLD 295
            +W+REM  LL V ++IVE IP  Q   +G+ +E+M  RPR+D+Y NLPALRKLD ML++
Sbjct: 161 ALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLME 220

Query: 296 ILDSFVDSEFWYVDQGIL---AAEADGSSFRK-LLERQEDKWWLPVPRVPSGGLSEASRR 355
            LDSF ++EFWY ++G L   +A +   SFRK +++R+E+KWWLPVP VPS GLS+ +R+
Sbjct: 221 ALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARK 280

Query: 356 QLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHIS-SDEFS 415
           QL++KR+ TNQI KAAMAINS  L++M++PDSY+  LPK G++S+G+ IYR++S S  F 
Sbjct: 281 QLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFF 340

Query: 416 PVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVID 475
           P  LL+CL++SSEH+A+++A+RVEA+MY WR+         S  S++K+SW M+K+L+  
Sbjct: 341 PEQLLDCLNISSEHEAVQLADRVEASMYTWRR--------KSCLSNSKNSWNMVKDLMST 400

Query: 476 ---ADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLA 535
               DK+ + AERAE++L+CLKQ++P L QT+LD+ KIQ+NKDVGKA+LESYSRVLE LA
Sbjct: 401 TERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLA 460

Query: 536 FNIVARIDDLLYVDDLAKHSD 548
           FNIVA IDD+LYVD   + S+
Sbjct: 461 FNIVAWIDDVLYVDKTMRGSE 473

BLAST of MC05g1504 vs. NCBI nr
Match: XP_022145651.1 (rop guanine nucleotide exchange factor 7-like isoform X2 [Momordica charantia])

HSP 1 Score: 1310 bits (3391), Expect = 0.0
Identity = 655/655 (100.00%), Postives = 655/655 (100.00%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV 300

Query: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360
           DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN
Sbjct: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360

Query: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420
           QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS
Sbjct: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420

Query: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480
           SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA
Sbjct: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480

Query: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540
           ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD
Sbjct: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540

Query: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHRGI 600
           DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHRGI
Sbjct: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHRGI 600

Query: 601 GVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERSKFGKTQDFSKCGDSIMN 655
           GVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERSKFGKTQDFSKCGDSIMN
Sbjct: 601 GVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERSKFGKTQDFSKCGDSIMN 655

BLAST of MC05g1504 vs. NCBI nr
Match: XP_022145650.1 (rop guanine nucleotide exchange factor 7-like isoform X1 [Momordica charantia])

HSP 1 Score: 1295 bits (3352), Expect = 0.0
Identity = 655/683 (95.90%), Postives = 655/683 (95.90%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLE----------------------------IMTC 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLE                            IMTC
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEVQFKISKYLFTKFLSLQLFFIFFLLADQIMTC 300

Query: 301 RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360
           RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW
Sbjct: 301 RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360

Query: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420
           WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR
Sbjct: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420

Query: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480
           ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL
Sbjct: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480

Query: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540
           RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK
Sbjct: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540

Query: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 600
           AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF
Sbjct: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 600

Query: 601 SSSPYTTTFTKPSFSPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAED 655
           SSSPYTTTFTKPSFSPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAED
Sbjct: 601 SSSPYTTTFTKPSFSPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAED 660

BLAST of MC05g1504 vs. NCBI nr
Match: XP_038905547.1 (rop guanine nucleotide exchange factor 7-like isoform X2 [Benincasa hispida])

HSP 1 Score: 809 bits (2090), Expect = 3.56e-288
Identity = 441/613 (71.94%), Postives = 488/613 (79.61%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQ Q+  PI   NHS           DC NPFS L  WV KSARK+     F +
Sbjct: 1   MDLTFTHQ-QDTEPIPLTNHSP-------TRSDCFNPFSNLGYWVSKSARKLCQKARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI     EGKEEKCD+FGN+ KVFDE  G
Sbjct: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EGKEEKCDVFGNSQKVFDENTG 120

Query: 121 DGESASSSSSDLLTSEATGS------HDEESSSSTL-------MGWPLNQTVGEEGGGDK 180
                S+SSSDLLTS+ TG        DEESSSS+        MGWPL++T GE   G  
Sbjct: 121 -----STSSSDLLTSKETGEMEEQRMQDEESSSSSSSSSSAASMGWPLHKTGGEGEEG-- 180

Query: 181 LHFDDRELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFG 240
                  +ERE S+ SE E+M ERFSKLLLGEDMSGCGNGVSTALAISNAITNLC ++FG
Sbjct: 181 -------VEREISTDSETEMMKERFSKLLLGEDMSGCGNGVSTALAISNAITNLCGSVFG 240

Query: 241 QLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYAN 300
           QLWRLEPL AERK MW REME LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPRSDLY N
Sbjct: 241 QLWRLEPLEAERKAMWEREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVN 300

Query: 301 LPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEA--DGSSFRKLLERQEDKWWLPVPRV 360
           LPALRKLDHMLL ILDSFVDSEFWYVDQGILAA+     SSFRK+LERQ++KWWLP+PRV
Sbjct: 301 LPALRKLDHMLLGILDSFVDSEFWYVDQGILAADQVDASSSFRKVLERQDEKWWLPIPRV 360

Query: 361 PSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGI 420
           P+GGLSE+S RQL HKRDCTNQILKAAMAINS+TLA+MD+P SYLE+LPKNGRASLGE I
Sbjct: 361 PNGGLSESSTRQLHHKRDCTNQILKAAMAINSLTLAEMDIPISYLESLPKNGRASLGEAI 420

Query: 421 YRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSS 480
           Y+HISSDEFSPV LLECLD+SSEHQAIEIANRVE AMY WRKG+ S   NNS     KSS
Sbjct: 421 YKHISSDEFSPVWLLECLDISSEHQAIEIANRVEGAMYVWRKGIAS---NNS-----KSS 480

Query: 481 WEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYS 540
           WEMLKELVID DK E+ AERAE VL CLKQ+FPNLPQT+LDMSKIQ+NKDVGKAILESYS
Sbjct: 481 WEMLKELVIDGDKIEVLAERAELVLLCLKQRFPNLPQTSLDMSKIQYNKDVGKAILESYS 540

Query: 541 RVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTT 592
           RVLESLA+NIVARI+DLLYV+++ KHSD+IP ISQLGIVAHN+SNRIQ SVPFSSSPYTT
Sbjct: 541 RVLESLAYNIVARIEDLLYVNEMTKHSDQIPGISQLGIVAHNSSNRIQISVPFSSSPYTT 572

BLAST of MC05g1504 vs. NCBI nr
Match: KAG6596381.1 (Rop guanine nucleotide exchange factor 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027930.1 Rop guanine nucleotide exchange factor 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 793 bits (2049), Expect = 3.74e-282
Identity = 443/608 (72.86%), Postives = 489/608 (80.43%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQE ER P+   NHSL     N    D  NPF  L  WV KS++K+     F +
Sbjct: 1   MDLIFTHQE-ERQPVPITNHSL-----NRSGSDRFNPFLTLSCWVSKSSQKLRHRARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCC G+EYKGMVVNNSTFCSSP FLEEVA+E          EKCD FG+A KVFDEK G
Sbjct: 61  GFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETG--------EKCDGFGDARKVFDEKTG 120

Query: 121 DGESA-SSSSSDLLTSEATG----SHDEESSSS---TLMGWPLNQTVGEEGGGDKLHFDD 180
            GES+ S SSSDLL+SEATG    SH+EESSSS   TLMGWPL++T  EE      +FD 
Sbjct: 121 VGESSLSDSSSDLLSSEATGHEGQSHEEESSSSSSSTLMGWPLHRTAAEER-----NFDG 180

Query: 181 RELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240
           R+LE + S VSE E+M ERF+KLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL
Sbjct: 181 RQLETQVSPVSETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240

Query: 241 EPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALR 300
           +PLA   K MW+REME LL V+NHIV+LIPV QTFPDG+KLEIMT RPRSDLY +LPALR
Sbjct: 241 QPLAPHIKAMWQREMELLLSVTNHIVQLIPVSQTFPDGTKLEIMTSRPRSDLYVSLPALR 300

Query: 301 KLDHMLLDILDSFVDSEFWYVDQGILAA-EADGSSFRKLLERQEDKWWLPVPRVPSG-GL 360
           KLDHMLLDILDSFVDSEFWYVDQGILAA +AD SS      RQEDKWWLP+PRVPSG GL
Sbjct: 301 KLDHMLLDILDSFVDSEFWYVDQGILAAADADRSS-----SRQEDKWWLPIPRVPSGSGL 360

Query: 361 SEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHIS 420
           SEA+RRQLQHKRD TNQILKA MAINS+TLADMDVP SYLEALPKNGR SLGE IYR+IS
Sbjct: 361 SEAARRQLQHKRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYIS 420

Query: 421 SDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLK 480
           SDEFSPV +LECLD+SSEHQA+EIANRVEAAMYAWR         NS RS++KSSWEMLK
Sbjct: 421 SDEFSPVYVLECLDISSEHQAVEIANRVEAAMYAWR---------NSSRSTSKSSWEMLK 480

Query: 481 ELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLES 540
           ELV+DA+KS++FAERAES+L CLKQQFPNLPQT+LDMSKIQ NKDVGKAILESYSRVLES
Sbjct: 481 ELVVDAEKSQVFAERAESLLLCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLES 540

Query: 541 LAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKP 592
           LAFNIVARIDDL+YVDDL KHSD+IP ISQLGI     S RIQF VPFSSSP    FTKP
Sbjct: 541 LAFNIVARIDDLVYVDDLTKHSDQIPGISQLGI-----SGRIQFPVPFSSSP----FTKP 566

BLAST of MC05g1504 vs. NCBI nr
Match: XP_022971231.1 (rop guanine nucleotide exchange factor 7-like [Cucurbita maxima])

HSP 1 Score: 793 bits (2049), Expect = 3.88e-282
Identity = 438/609 (71.92%), Postives = 490/609 (80.46%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQE ER P+   NHSL     N ++ D  NPF  L  WV KSA+K+     F +
Sbjct: 1   MDLIFTHQE-EREPVPITNHSL-----NRISSDRFNPFLTLSYWVSKSAQKLRHRARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCC G+EYKGMVVNNSTFCSSP FLEEVA+E          EKCD FG+A KVFDEK G
Sbjct: 61  GFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETG--------EKCDGFGDALKVFDEKSG 120

Query: 121 DGESA-SSSSSDLLTSEATG----SHDEE-----SSSSTLMGWPLNQTVGEEGGGDKLHF 180
            GES+ S SS DLL+SEATG    SH+EE     SSSSTLMGWPL++T  EE      +F
Sbjct: 121 VGESSLSDSSPDLLSSEATGHEGQSHEEEPSSSSSSSSTLMGWPLHRTAAEER-----NF 180

Query: 181 DDRELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLW 240
           DDR+LE + S VSE E+M ERF+KLLLGEDMSGCGNGVSTALAISNAITNLCA+LFGQLW
Sbjct: 181 DDRQLETQVSPVSETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCASLFGQLW 240

Query: 241 RLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPA 300
           RL+PLA  +K MW+REME LL V+NHIV+LIP+ QTFPDG+KLEIMT RPRSDLY +LPA
Sbjct: 241 RLQPLAPHKKAMWQREMELLLSVTNHIVQLIPISQTFPDGTKLEIMTSRPRSDLYVSLPA 300

Query: 301 LRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSG-G 360
           LRKLD MLLDILDSFVDSEFWYVDQGILAA+ D SS      RQEDKWWLP+PRVPSG G
Sbjct: 301 LRKLDRMLLDILDSFVDSEFWYVDQGILAADTDRSS-----SRQEDKWWLPIPRVPSGSG 360

Query: 361 LSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHI 420
           LSEA+RRQLQHKRD TNQILKA MAINS+TLADMDVP SYLEALPKNGR SLGE IYR+I
Sbjct: 361 LSEAARRQLQHKRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYI 420

Query: 421 SSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEML 480
           SSDEFSPV +LEC+D+SSEHQA+EIANRVEAAMYAWR         NS RS++KSSWEML
Sbjct: 421 SSDEFSPVYVLECVDISSEHQAVEIANRVEAAMYAWR---------NSSRSTSKSSWEML 480

Query: 481 KELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 540
           KELV+DA+KS++FAERAESVL+CLKQQFPNLPQT+LDMSKIQ NKDVGKAILESYSRVLE
Sbjct: 481 KELVVDAEKSQVFAERAESVLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLE 540

Query: 541 SLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTK 592
           SLAFNIVARIDDL+YVDDL KHSD+IP ISQLG+     S RIQF VPFSSSP    FTK
Sbjct: 541 SLAFNIVARIDDLVYVDDLTKHSDQIPGISQLGV-----SGRIQFPVPFSSSP----FTK 567

BLAST of MC05g1504 vs. ExPASy TrEMBL
Match: A0A6J1CWY5 (rop guanine nucleotide exchange factor 7-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 1310 bits (3391), Expect = 0.0
Identity = 655/655 (100.00%), Postives = 655/655 (100.00%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV 300

Query: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360
           DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN
Sbjct: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360

Query: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420
           QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS
Sbjct: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420

Query: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480
           SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA
Sbjct: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480

Query: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540
           ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD
Sbjct: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540

Query: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHRGI 600
           DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHRGI
Sbjct: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHRGI 600

Query: 601 GVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERSKFGKTQDFSKCGDSIMN 655
           GVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERSKFGKTQDFSKCGDSIMN
Sbjct: 601 GVNNESIDNVASMKGCGNFIEKLDLVAEIAEDEGSERSKFGKTQDFSKCGDSIMN 655

BLAST of MC05g1504 vs. ExPASy TrEMBL
Match: A0A6J1CV30 (rop guanine nucleotide exchange factor 7-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 1295 bits (3352), Expect = 0.0
Identity = 655/683 (95.90%), Postives = 655/683 (95.90%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLE----------------------------IMTC 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLE                            IMTC
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEVQFKISKYLFTKFLSLQLFFIFFLLADQIMTC 300

Query: 301 RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360
           RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW
Sbjct: 301 RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360

Query: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420
           WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR
Sbjct: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420

Query: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480
           ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL
Sbjct: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480

Query: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540
           RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK
Sbjct: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540

Query: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 600
           AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF
Sbjct: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 600

Query: 601 SSSPYTTTFTKPSFSPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAED 655
           SSSPYTTTFTKPSFSPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAED
Sbjct: 601 SSSPYTTTFTKPSFSPIPIISQTPHRGIGVNNESIDNVASMKGCGNFIEKLDLVAEIAED 660

BLAST of MC05g1504 vs. ExPASy TrEMBL
Match: A0A6J1I2R8 (rop guanine nucleotide exchange factor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111470019 PE=4 SV=1)

HSP 1 Score: 793 bits (2049), Expect = 1.88e-282
Identity = 438/609 (71.92%), Postives = 490/609 (80.46%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQE ER P+   NHSL     N ++ D  NPF  L  WV KSA+K+     F +
Sbjct: 1   MDLIFTHQE-EREPVPITNHSL-----NRISSDRFNPFLTLSYWVSKSAQKLRHRARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCC G+EYKGMVVNNSTFCSSP FLEEVA+E          EKCD FG+A KVFDEK G
Sbjct: 61  GFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETG--------EKCDGFGDALKVFDEKSG 120

Query: 121 DGESA-SSSSSDLLTSEATG----SHDEE-----SSSSTLMGWPLNQTVGEEGGGDKLHF 180
            GES+ S SS DLL+SEATG    SH+EE     SSSSTLMGWPL++T  EE      +F
Sbjct: 121 VGESSLSDSSPDLLSSEATGHEGQSHEEEPSSSSSSSSTLMGWPLHRTAAEER-----NF 180

Query: 181 DDRELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLW 240
           DDR+LE + S VSE E+M ERF+KLLLGEDMSGCGNGVSTALAISNAITNLCA+LFGQLW
Sbjct: 181 DDRQLETQVSPVSETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCASLFGQLW 240

Query: 241 RLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPA 300
           RL+PLA  +K MW+REME LL V+NHIV+LIP+ QTFPDG+KLEIMT RPRSDLY +LPA
Sbjct: 241 RLQPLAPHKKAMWQREMELLLSVTNHIVQLIPISQTFPDGTKLEIMTSRPRSDLYVSLPA 300

Query: 301 LRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSG-G 360
           LRKLD MLLDILDSFVDSEFWYVDQGILAA+ D SS      RQEDKWWLP+PRVPSG G
Sbjct: 301 LRKLDRMLLDILDSFVDSEFWYVDQGILAADTDRSS-----SRQEDKWWLPIPRVPSGSG 360

Query: 361 LSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHI 420
           LSEA+RRQLQHKRD TNQILKA MAINS+TLADMDVP SYLEALPKNGR SLGE IYR+I
Sbjct: 361 LSEAARRQLQHKRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYI 420

Query: 421 SSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEML 480
           SSDEFSPV +LEC+D+SSEHQA+EIANRVEAAMYAWR         NS RS++KSSWEML
Sbjct: 421 SSDEFSPVYVLECVDISSEHQAVEIANRVEAAMYAWR---------NSSRSTSKSSWEML 480

Query: 481 KELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 540
           KELV+DA+KS++FAERAESVL+CLKQQFPNLPQT+LDMSKIQ NKDVGKAILESYSRVLE
Sbjct: 481 KELVVDAEKSQVFAERAESVLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLE 540

Query: 541 SLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTK 592
           SLAFNIVARIDDL+YVDDL KHSD+IP ISQLG+     S RIQF VPFSSSP    FTK
Sbjct: 541 SLAFNIVARIDDLVYVDDLTKHSDQIPGISQLGV-----SGRIQFPVPFSSSP----FTK 567

BLAST of MC05g1504 vs. ExPASy TrEMBL
Match: A0A6J1FND9 (rop guanine nucleotide exchange factor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111447031 PE=4 SV=1)

HSP 1 Score: 792 bits (2045), Expect = 7.34e-282
Identity = 442/608 (72.70%), Postives = 489/608 (80.43%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQE ER P+   NHSL     N    D  NPF  L  WV KS++K+     F +
Sbjct: 1   MDLIFTHQE-ERQPVPITNHSL-----NRSGSDRFNPFLTLSCWVSKSSQKLRHRARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCC G+EYKGMVVNNSTFCSSP FLEEVA+E          EKCD FG+A KVFDEK G
Sbjct: 61  GFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIETG--------EKCDGFGDARKVFDEKTG 120

Query: 121 DGESA-SSSSSDLLTSEATG----SHDEESSSS---TLMGWPLNQTVGEEGGGDKLHFDD 180
            GES+ S SSSDLL+SEATG    SH+EESSSS   TLMGWPL++T  EE      +FD 
Sbjct: 121 VGESSLSDSSSDLLSSEATGHEGQSHEEESSSSSSSTLMGWPLHRTAAEER-----NFDG 180

Query: 181 RELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240
           R+LE + S VSE E+M ERF+KLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL
Sbjct: 181 RQLETQVSPVSETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRL 240

Query: 241 EPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALR 300
           +PLA+  K MW+REME LL V+NHIV+LIPV QTFPDG+KLEIMT RPRSDLY +LPALR
Sbjct: 241 QPLASHIKAMWQREMELLLSVTNHIVQLIPVSQTFPDGTKLEIMTSRPRSDLYVSLPALR 300

Query: 301 KLDHMLLDILDSFVDSEFWYVDQGILAA-EADGSSFRKLLERQEDKWWLPVPRVPSG-GL 360
           KLDHMLLDILDSFVDS FWYVDQGILAA +AD SS      RQEDKWWLP+PRVPSG GL
Sbjct: 301 KLDHMLLDILDSFVDSAFWYVDQGILAAADADRSS-----SRQEDKWWLPIPRVPSGSGL 360

Query: 361 SEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHIS 420
           SEA+RRQLQHKRD TNQILKA MAINS+TLADMDVP SYLEALPKNGR SLGE IYR+IS
Sbjct: 361 SEAARRQLQHKRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYIS 420

Query: 421 SDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLK 480
           SDEFSPV +LECLD+SSEHQA+EIANRVEAAMYAWR         NS RS++KSSWEMLK
Sbjct: 421 SDEFSPVYVLECLDISSEHQAVEIANRVEAAMYAWR---------NSSRSTSKSSWEMLK 480

Query: 481 ELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLES 540
           ELV+DA+KS++FAERAES+L CLKQQFPNLPQT+LDMSKIQ NKDVGKAILESYSRVLES
Sbjct: 481 ELVVDAEKSQVFAERAESLLLCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLES 540

Query: 541 LAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKP 592
           LAFNIVARIDDL+YVDDL KHSD+IP ISQLGI     S RIQF VPFSSSP    FTKP
Sbjct: 541 LAFNIVARIDDLVYVDDLTKHSDQIPGISQLGI-----SGRIQFPVPFSSSP----FTKP 566

BLAST of MC05g1504 vs. ExPASy TrEMBL
Match: A0A5A7TMM7 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004810 PE=4 SV=1)

HSP 1 Score: 792 bits (2046), Expect = 1.11e-281
Identity = 442/620 (71.29%), Postives = 488/620 (78.71%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVF-----PS 60
           MD+ F HQ Q   PI   NHS           DC NPFS L  WV KSARK+       +
Sbjct: 1   MDLTFTHQ-QHIEPIPLTNHSP-------TPSDCFNPFSNLGNWVSKSARKLCHRARSST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI     E KEEKCD+F NA KV DEK  
Sbjct: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EAKEEKCDVFENARKVSDEK-- 120

Query: 121 DGESASSSSSDLLTS------EATGSHDEESSSSTL-------------MGWPLNQTVGE 180
              +AS+SSSDLL S      E   S DEESSSS+              MGWPL++ VGE
Sbjct: 121 ---TASNSSSDLLKSVETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGE 180

Query: 181 EGGGDKLHFDDRELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNL 240
                    ++  +E+E S+ SEME+M ERFSKLLLGEDMSG GNGV TALAISNAITNL
Sbjct: 181 --------GEEDGVEKEVSTDSEMEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNL 240

Query: 241 CATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPR 300
           C ++FGQLWRLEPL AERK MW REME+LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPR
Sbjct: 241 CGSVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPR 300

Query: 301 SDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAE-ADGSS-FRKLLERQEDKWW 360
           SDLY NLPALRKLDHMLLD+LDSFVDSEFWY+DQGILA + AD SS FRKLLERQ+DKWW
Sbjct: 301 SDLYVNLPALRKLDHMLLDMLDSFVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWW 360

Query: 361 LPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRA 420
           LP+PRVP+GGLSEAS R L HKRDCTNQILKAAMAINSVTLADMDVP SYLEALPKNGRA
Sbjct: 361 LPIPRVPNGGLSEASTRHLHHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRA 420

Query: 421 SLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWR-KGMGSKPTNNSL 480
           SLGE IY++ISSDEFSP  LLECLD+SSEHQAIEIANRVE+AMYAWR KG+ S   NNS 
Sbjct: 421 SLGEAIYKYISSDEFSPAWLLECLDISSEHQAIEIANRVESAMYAWRSKGIAS---NNS- 480

Query: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540
               KSSWEMLKEL+IDADKSE+ AERAE VL CLKQQFPNLPQT+LDMSKIQ+NKDVGK
Sbjct: 481 ----KSSWEMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGK 540

Query: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 592
           AILESYSRVLESLA+NIVARI+DLLYV++L KHSD+IP ISQLGIVAHN+SNRIQ SVPF
Sbjct: 541 AILESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIQISVPF 580

BLAST of MC05g1504 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 591.7 bits (1524), Expect = 7.4e-169
Identity = 320/484 (66.12%), Postives = 377/484 (77.89%), Query Frame = 0

Query: 111 DEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG-WPLNQTVGEEGGGDKLHFDDRE 170
           +EK  +    SS ++     E + S  E+ ++S +   W +    GE+    K+  D R 
Sbjct: 12  EEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEK---KKIRSDSR- 71

Query: 171 LEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEP 230
                  VSE+E+M ERFSKLLLGEDMSG GNGV TALAISNAITNLCATLFGQLWRLEP
Sbjct: 72  -------VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEP 131

Query: 231 LAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKL 290
           L  E+K MWRREMEWLLCVS+HIVE+ P WQTFPDG+KLEIMTCRPRSDLY NLPALRKL
Sbjct: 132 LPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKL 191

Query: 291 DHMLLDILDSFVDSEFWYVDQGILAAE--ADG-SSFRKLLERQEDKWWLPVPRVPSGGLS 350
           D+MLL+ILDSF ++EFWYVDQGI+A E  ADG SSFRK  +RQEDKWWLPVPRV  GGL 
Sbjct: 192 DNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQ 251

Query: 351 EASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISS 410
           E SR+QLQHKRDCTNQILKAAMAINS+TLADM++P+SYLE+LP+ GR+ LG+ IYR+ISS
Sbjct: 252 ENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISS 311

Query: 411 DEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKE 470
           D+FSP CLL+CLD+SSEHQAIEIANRVE+++Y W K   SKP  N+     K+SWEM+KE
Sbjct: 312 DQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNT-----KTSWEMVKE 371

Query: 471 LVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESL 530
           L++DADK EL A+RAES+L  LKQ+FP LPQT LDMSKIQ+NKD+GK+ILESYSRVLESL
Sbjct: 372 LMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESL 431

Query: 531 AFNIVARIDDLLYVDDLAKH-SDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKP 589
           AFNIVARIDDLL+VDDL +H SD+IP  + LG    N  N    S+    S YTT    P
Sbjct: 432 AFNIVARIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTT----P 469

BLAST of MC05g1504 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 536.2 bits (1380), Expect = 3.7e-152
Identity = 298/596 (50.00%), Postives = 400/596 (67.11%), Query Frame = 0

Query: 86  MEIRVEDKEGKEEK-CDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSST 145
           ME  V+   G E+K  ++  +   +  E + +  S S +S +  ++    S       S 
Sbjct: 1   MENLVKSCAGIEKKRSNLTPSMEDILTESK-ERSSTSGASYESSSTTTVASSSPPPPPSQ 60

Query: 146 LMGWPLNQTVGEEGGGDKLHFDDREL---------EREASSVSEMEIMERFSKLLLGEDM 205
           ++GWP+ +    +   + ++FD ++L          +E +S     + ERF+KLLLGEDM
Sbjct: 61  ILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDM 120

Query: 206 SGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELI 265
           SG G GV TALAISNAITNLCAT+FGQLWRLEPL++E+K MWRREMEW+L VS+HIVEL 
Sbjct: 121 SGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELT 180

Query: 266 PVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAE 325
           P  QT+PDG+K E+MTCRPR DL+ NLPALRKLD+MLLDIL SF  +EFWYVDQGI+A+E
Sbjct: 181 PSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASE 240

Query: 326 ADGS-SFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTL 385
            DGS SFR+ ++RQE+KWWLPVPR+   GL+E +R +L HKR+C  QILKAAMAINS+ L
Sbjct: 241 NDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLAL 300

Query: 386 ADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEA 445
            +MDVP SYLE LPKNGR+ LG+ IYR+++SD+FS   LL+CLD+SSEH A++IANRVEA
Sbjct: 301 TEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEA 360

Query: 446 AMYAWRKGMGSK--PTNNSLRSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFP 505
           ++Y WR+ + +K    NN+  ++ K +WEM+KEL+   DK  L  ER+E++L CLKQ+FP
Sbjct: 361 SIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFP 420

Query: 506 NLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRI 565
           +L QT+LD+SKIQ NKD+GK+ILESYSR LESLA NI+ARIDDLLYVDDL K SD+   +
Sbjct: 421 SLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLL 480

Query: 566 SQ---LGIVAHNTSNRIQFSVPFSSSPYTTTF-TKPSFSPIPIISQ-------------- 625
           S      I+AH     + + +  S +PY T+F T P FSP  I  +              
Sbjct: 481 SSPAVSSIIAHKKVVPLPYLISASGTPYRTSFSTTPGFSPSMISPKKGERRTPYSSKDTN 540

Query: 626 ------TPHRGIGVN---NESIDNVASMKGCGNFIEKLD--LVAEIAEDEGSERSK 640
                  P RG GV    N  +   + +K C N  +  D  ++ +I++D   E+ +
Sbjct: 541 KIIEKGLPSRGYGVRRVLNNYLGMESKLKICVNPSDNADTAVINQISKDVEEEKKR 595

BLAST of MC05g1504 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 471.1 bits (1211), Expect = 1.5e-132
Identity = 271/510 (53.14%), Postives = 358/510 (70.20%), Query Frame = 0

Query: 119 SASSSSSDLLTSEATGSHDEESSS-----STLMGWPLNQTVGEEGGG-----DKLHFDDR 178
           S SSSSS + +S    S  E S S     S ++GWPL Q    +  G          DD 
Sbjct: 37  SLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDF 96

Query: 179 ELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLE 238
             +R  +  SE+E++ ER +KLLLGEDMSG G GV  ALAISNAITNL A + GQ WRLE
Sbjct: 97  GFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLE 156

Query: 239 PLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRK 298
           P+ +E+K MWRRE+E LL VS+HIVEL+P +Q FP+G+K+E+M CRPRSDL+  LPALRK
Sbjct: 157 PIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRK 216

Query: 299 LDHMLLDILDSFVDSEFWYVDQGILAAE-ADGSSFRKLLERQEDKWWLPVPRVPSGGLSE 358
           LD+ML++ILDSF ++EFWYVDQGI+AAE A  +SFR+      DKWWLP+PRVPS GL+E
Sbjct: 217 LDNMLIEILDSFGETEFWYVDQGIVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTE 276

Query: 359 ASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSD 418
            +R++L H R+ TNQILKA M+INS+ LA+M+VP SYLEALPKNGR+ LG+ +YR+I+SD
Sbjct: 277 QTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSD 336

Query: 419 EFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKEL 478
            FS   LLE +D+SSE   +E+ANRVEA+MY WR+   S+   +  RS++     ++KE+
Sbjct: 337 NFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEM 396

Query: 479 VI---DADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 538
           ++   D DK E+FAERAES+L  LKQ+FP L QT LD SKIQ+NKDVGK+ILESYSRVLE
Sbjct: 397 MMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLE 456

Query: 539 SLAFNIVARIDDLLYVDDLAKH---------SDEIPRISQ------LGIVAHNTSNRIQF 594
           SLA++I  RI+++L++DD++K          SD++  +S+       G +    S    F
Sbjct: 457 SLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLF 516

BLAST of MC05g1504 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 467.6 bits (1202), Expect = 1.6e-131
Identity = 262/515 (50.87%), Postives = 349/515 (67.77%), Query Frame = 0

Query: 91  EDKEGKEEKCDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG--- 150
           ED E   E+C  +  +  +        ES SSSS      +  G+     SS  ++    
Sbjct: 8   EDDEVSSERCGSYSPSADI-------SESESSSSFSCHRFDGEGASSSIPSSPRVVAGRG 67

Query: 151 --WPLNQTVGEEGGGDKLHFDDRELEREASSVSEMEIMERFSKLLLGEDMSGCGNGVSTA 210
             +P    +   GG D + +DD++ + + S +  M+  ERF+KLLLGEDMSG G GV TA
Sbjct: 68  FYFPAPVMLPVIGGKDVV-WDDKQPDNDLSEIEMMK--ERFAKLLLGEDMSGGGKGVCTA 127

Query: 211 LAISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGS 270
           LAISNAITNL AT+FG+LWRLEPLA ++K MWRRE+EWLLCVS+ IVELIP  Q FP G 
Sbjct: 128 LAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGG 187

Query: 271 KLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLL 330
             EIM  RPRSDLYANLPAL+KLD ML+D+LD+F D+EFWY D+GI+  + D  S+    
Sbjct: 188 TYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPA 247

Query: 331 E-RQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYL 390
             RQEDKWWLP P+VP  GLSE +R++LQ  RD  NQILKAA+AINS  LA+M++PD YL
Sbjct: 248 SVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYL 307

Query: 391 EALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMG 450
           E LPK+G+  LGE IY+++++++FSP CLL+CLD+SSEHQ +EIANR+EAA++ WR+  G
Sbjct: 308 ETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNG 367

Query: 451 SKPTNNSLRSSAKSSW-EMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSK 510
            +  +        SSW   +K LV D ++++   +RAE++L  L+ +FP LPQT LDM+K
Sbjct: 368 RR--HKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNK 427

Query: 511 IQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTS 570
           IQ+NKDVG++ILESYSRV+ES+AFNI ARIDD+LYVDD  + S  I     L + + N  
Sbjct: 428 IQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRS--ISVTESLSLFSINGL 487

Query: 571 NRIQFSVPFSSSPYTTTFTKPSFSPIPIISQTPHR 599
           N  Q +    SSP+ + F  P+ S   + S++P R
Sbjct: 488 NP-QKAFSVQSSPHGSPFATPALS---VASRSPRR 504

BLAST of MC05g1504 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 423.3 bits (1087), Expect = 3.5e-118
Identity = 217/381 (56.96%), Postives = 293/381 (76.90%), Query Frame = 0

Query: 176 SVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERK 235
           S  E+E M ERF+KLLLGEDMSG G GV TA+ ISNAITNL AT+FGQ  RLEPL  E++
Sbjct: 101 SEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKR 160

Query: 236 GMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLD 295
            +W+REM  LL V ++IVE IP  Q   +G+ +E+M  RPR+D+Y NLPALRKLD ML++
Sbjct: 161 ALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLME 220

Query: 296 ILDSFVDSEFWYVDQGIL---AAEADGSSFRK-LLERQEDKWWLPVPRVPSGGLSEASRR 355
            LDSF ++EFWY ++G L   +A +   SFRK +++R+E+KWWLPVP VPS GLS+ +R+
Sbjct: 221 ALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARK 280

Query: 356 QLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHIS-SDEFS 415
           QL++KR+ TNQI KAAMAINS  L++M++PDSY+  LPK G++S+G+ IYR++S S  F 
Sbjct: 281 QLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFF 340

Query: 416 PVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVID 475
           P  LL+CL++SSEH+A+++A+RVEA+MY WR+         S  S++K+SW M+K+L+  
Sbjct: 341 PEQLLDCLNISSEHEAVQLADRVEASMYTWRR--------KSCLSNSKNSWNMVKDLMST 400

Query: 476 ---ADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLA 535
               DK+ + AERAE++L+CLKQ++P L QT+LD+ KIQ+NKDVGKA+LESYSRVLE LA
Sbjct: 401 TERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLA 460

Query: 536 FNIVARIDDLLYVDDLAKHSD 548
           FNIVA IDD+LYVD   + S+
Sbjct: 461 FNIVAWIDDVLYVDKTMRGSE 473