MC05g1489 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1489
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	tropinone reductase homolog
Location	MC05: 18821374 .. 18823049 (+)
RNA-Seq Expression	MC05g1489
Synteny	MC05g1489

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTTCAACAACTCCGACTAAAAAAAGCTGCGAGTTGAAATCACCGCACACACATGTTATTCAATCATATCTTTTAATGGCGTTGAATATGAATTAAATATCAAGAGGCTTCTTAGAGAAACAAACAAATGAATCAAGAGGGGGAAAAAAAAGAAAAAAAAAATTGTAAAAAAGTAATGAGACATATCTGAGAAGAGAAGGCACATGAATCACGTTGCTGCACACGAGTAAGATCCATTAGCGATGCTGTAATTAATTAGCGGCGTTGAATCAGAACGAACAGCAGATGGCGGAAGCAGAGCAGCGGAGCAGAGGCCAAAGATGGTCTCTGAAGGGGATGACCGCTCTCGTTACCGGCGGAACCCGCGGCATTGGGCGGGCCACGGTGGAAGAACTAGCGGCGTTCGGGGCCGTGGTTCACACGTGCTCTCGAAGCCAGCAAGATCTTGACCAATGCCTCAAAGAATGGCAAGAAATGGGGTTCGAAGTCACCGGATCTGTGTGCGATGTGCACATCAGAGAACAAAGGGAGAAGCTTATGGAAACCGTCTCCTCTCTCTTCAATGCCACACTCAACATTCTGGTGAAACCAAAAACATGCTCATAAATCCCAAAATCTGTTCGTGATTTGATTTTGGATGAGAGTATTTTTGTACTTTGCAGGTGAATAATGCAGGGACTATGGTGATCAAGAGCGCAGCAGAAATTACAGAGGCAGAGCTTTCGAGGGTAATGAGTACAAATTTCGAGGCCTCCTTCCATTTTTGCCAACTTGCACATCCGCTGCTCAAAGCTTCTGGCTATGGAAGCATTGTCTTCATCTCCTCTGTTTCAGGCTTGATGTCCCTTCCATTATCTACTGCCTATGCTGCATCCAAAGGTAACACATTTCACACTTTTTTTTTTTTTTTGGGAATTGACATTTTTGTACCACGTTTTGGACTTTAGGATTCTTTGTTACAAAGGGAGTTTCTCATAAGGGATTGTTTGTTAAGGAAGGTGCCATAAATCAAATCACGAAGAACTTGGCTTGTGAATGGGCAAAGGATAACATCAGAGCAAACGCAGTGGCTCCATGGATCATAAAAACTCGCTTTGTGGAACCTCCCTCTGTAAAATTTCAACTTTTCTTTTTGGATCTCTTTCGAATTTAATTGAACAAATTATATTATTGCTGATTTTTGTTTGTTTGTTTGTTTTGGTGACAGGATAATCCGGTACATGTTGAAACAATGAAGTACATGCTATCTGTGACACCTCTGAAACGAGGTGGAGAGCCACATGAAGTGTCATCGTTGGTGGCGTTTCTTTGTCTTCCAGCTGCGTCTTACATCACTGGCCAAGTTATTTGTGTTGACGGTGGCCATACTGTGAAGGCTTTCCCCTTCCCTGATCTTTGAAATTCTCCCAAGTTTAAGTTCTCTTTTTCACACTAGGGCATCTATTTATTCATGTTTGGTTGAAAATCTGGGAACATATCTTATCAATATTTATAGGTTCTATTTATTTCAACACTAATGTTATATACGAATTTAGGTGGAATATGTGGTGCCTCTACTAATTCCTTAGCGGTGAATACAAATAATTTATTCTTTTGCTAATTGGTTGTACAAGGCAAACTAGGTTTGAGTCTCGATAGCGATAGCATTTATGTTGTAATAGATGTATTAAATTTCC

mRNA sequence

AGTTCAACAACTCCGACTAAAAAAAGCTGCGAGTTGAAATCACCGCACACACATGTTATTCAATCATATCTTTTAATGGCGTTGAATATGAATTAAATATCAAGAGGCTTCTTAGAGAAACAAACAAATGAATCAAGAGGGGGAAAAAAAAGAAAAAAAAAATTGTAAAAAAGTAATGAGACATATCTGAGAAGAGAAGGCACATGAATCACGTTGCTGCACACGAGTAAGATCCATTAGCGATGCTGTAATTAATTAGCGGCGTTGAATCAGAACGAACAGCAGATGGCGGAAGCAGAGCAGCGGAGCAGAGGCCAAAGATGGTCTCTGAAGGGGATGACCGCTCTCGTTACCGGCGGAACCCGCGGCATTGGGCGGGCCACGGTGGAAGAACTAGCGGCGTTCGGGGCCGTGGTTCACACGTGCTCTCGAAGCCAGCAAGATCTTGACCAATGCCTCAAAGAATGGCAAGAAATGGGGTTCGAAGTCACCGGATCTGTGTGCGATGTGCACATCAGAGAACAAAGGGAGAAGCTTATGGAAACCGTCTCCTCTCTCTTCAATGCCACACTCAACATTCTGGTGAATAATGCAGGGACTATGGTGATCAAGAGCGCAGCAGAAATTACAGAGGCAGAGCTTTCGAGGGTAATGAGTACAAATTTCGAGGCCTCCTTCCATTTTTGCCAACTTGCACATCCGCTGCTCAAAGCTTCTGGCTATGGAAGCATTGTCTTCATCTCCTCTGTTTCAGGCTTGATGTCCCTTCCATTATCTACTGCCTATGCTGCATCCAAAGGTGCCATAAATCAAATCACGAAGAACTTGGCTTGTGAATGGGCAAAGGATAACATCAGAGCAAACGCAGTGGCTCCATGGATCATAAAAACTCGCTTTGTGGAACCTCCCTCTGATAATCCGGTACATGTTGAAACAATGAAGTACATGCTATCTGTGACACCTCTGAAACGAGGTGGAGAGCCACATGAAGTGTCATCGTTGGTGGCGTTTCTTTGTCTTCCAGCTGCGTCTTACATCACTGGCCAAGTTATTTGTGTTGACGGTGGCCATACTGTGAAGGCTTTCCCCTTCCCTGATCTTTGAAATTCTCCCAAGTTTAAGTTCTCTTTTTCACACTAGGGCATCTATTTATTCATGTTTGGTTGAAAATCTGGGAACATATCTTATCAATATTTATAGGTTCTATTTATTTCAACACTAATGTTATATACGAATTTAGGTGGAATATGTGGTGCCTCTACTAATTCCTTAGCGGTGAATACAAATAATTTATTCTTTTGCTAATTGGTTGTACAAGGCAAACTAGGTTTGAGTCTCGATAGCGATAGCATTTATGTTGTAATAGATGTATTAAATTTCC

Coding sequence (CDS)

ATGGCGGAAGCAGAGCAGCGGAGCAGAGGCCAAAGATGGTCTCTGAAGGGGATGACCGCTCTCGTTACCGGCGGAACCCGCGGCATTGGGCGGGCCACGGTGGAAGAACTAGCGGCGTTCGGGGCCGTGGTTCACACGTGCTCTCGAAGCCAGCAAGATCTTGACCAATGCCTCAAAGAATGGCAAGAAATGGGGTTCGAAGTCACCGGATCTGTGTGCGATGTGCACATCAGAGAACAAAGGGAGAAGCTTATGGAAACCGTCTCCTCTCTCTTCAATGCCACACTCAACATTCTGGTGAATAATGCAGGGACTATGGTGATCAAGAGCGCAGCAGAAATTACAGAGGCAGAGCTTTCGAGGGTAATGAGTACAAATTTCGAGGCCTCCTTCCATTTTTGCCAACTTGCACATCCGCTGCTCAAAGCTTCTGGCTATGGAAGCATTGTCTTCATCTCCTCTGTTTCAGGCTTGATGTCCCTTCCATTATCTACTGCCTATGCTGCATCCAAAGGTGCCATAAATCAAATCACGAAGAACTTGGCTTGTGAATGGGCAAAGGATAACATCAGAGCAAACGCAGTGGCTCCATGGATCATAAAAACTCGCTTTGTGGAACCTCCCTCTGATAATCCGGTACATGTTGAAACAATGAAGTACATGCTATCTGTGACACCTCTGAAACGAGGTGGAGAGCCACATGAAGTGTCATCGTTGGTGGCGTTTCTTTGTCTTCCAGCTGCGTCTTACATCACTGGCCAAGTTATTTGTGTTGACGGTGGCCATACTGTGAAGGCTTTCCCCTTCCCTGATCTTTGA

Protein sequence

MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPDL

Homology

[Close]

BLAST of MC05g1489 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 4.3e-91
Identity = 167/267 (62.55%), Postives = 204/267 (76.40%), Query Frame = 0


Query: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60
           MA  E     +RWSL+GMTALVTGGTRGIG A VEELA FGA V+TCSRSQ DLD+CL++
Sbjct: 1   MAGREIGGGDRRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEK 60

Query: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120
           W+  GF+V+G VCDV    QR+ LME+V+S FN  LNIL+NNAGT + K A   T  + S
Sbjct: 61  WRRKGFKVSGPVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIPKEATNFTAEDYS 120

Query: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN 180
            +M TNFEAS++ CQLAHPLLKASG  SIVF SS +G++++PLS+ YAASKGAINQ+TK+
Sbjct: 121 IIMGTNFEASYNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKS 180

Query: 181 LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV 240
           LACEWAKD+IR NAVAPWII T  +E     P   + ++ ++   P+KR GEP EVSSLV
Sbjct: 181 LACEWAKDSIRVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLV 240

Query: 241 AFLCLPAASYITGQVICVDGGHTVKAF 268
            +LCLP ASYITGQ+ICVDGG+TV  F
Sbjct: 241 TYLCLPTASYITGQIICVDGGYTVNGF 267

[Close]

BLAST of MC05g1489 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 1.9e-86
Identity = 162/259 (62.55%), Postives = 195/259 (75.29%), Query Frame = 0


Query: 11  QRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTG 70
           +RWSL G TALVTGGTRGIGRA VEELA FGA VHTCSR+Q++L+ CL +W+  G  V+G
Sbjct: 5   KRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSG 64

Query: 71  SVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEAS 130
           SVCD  +R+QREKL++  SS F+  LNIL+NN GT V K   E +  E +++MSTN E++
Sbjct: 65  SVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESA 124

Query: 131 FHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNI 190
           FH  Q+AHPLLKASG GSIVFISSV+GL+ L   + Y A+KGA+NQ+T+NLACEWA DNI
Sbjct: 125 FHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNI 184

Query: 191 RANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPAASY 250
           R N VAPW IKT  VE   +    VE    ++S TPL R GEP EVSSLVAFLCLPA+SY
Sbjct: 185 RTNCVAPWYIKTSLVETLLEKKEFVEA---VVSRTPLGRVGEPEEVSSLVAFLCLPASSY 244

Query: 251 ITGQVICVDGGHTVKAFPF 270
           ITGQVI VDGG TV  F +
Sbjct: 245 ITGQVISVDGGFTVNGFSY 260

[Close]

BLAST of MC05g1489 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.2e-86
Identity = 163/260 (62.69%), Postives = 195/260 (75.00%), Query Frame = 0


Query: 11  QRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTG 70
           +RWSL GM+ALVTGGTRGIGRA VEELA  GA VHTC+R++ +L+ CL +W   GF V G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 71  SVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEAS 130
           SVCDV  R QRE LMETVSS+F   L+ILVNN GT + K   E T  E S +MSTNFE+ 
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESV 183

Query: 131 FHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNI 190
           FH CQLA+PLL+ S  GS+VFISSVSG +SL   +  +++KGAINQ+T++LACEWAKDNI
Sbjct: 184 FHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNI 243

Query: 191 RANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPAASY 250
           R NAVAPW IKT  VE    N  ++E    + SVTPL R GEP EVSS VAFLCLPA+SY
Sbjct: 244 RINAVAPWYIKTSMVEQVLSNKEYLEE---VYSVTPLGRLGEPREVSSAVAFLCLPASSY 303

Query: 251 ITGQVICVDGGHTVKAFPFP 271
           ITGQ++CVDGG ++  F FP
Sbjct: 304 ITGQILCVDGGMSINGF-FP 319

[Close]

BLAST of MC05g1489 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 3.3e-83
Identity = 157/260 (60.38%), Postives = 195/260 (75.00%), Query Frame = 0


Query: 11  QRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTG 70
           +RWSL+GM ALVTGGT+GIG A VEEL+  GA VHTC+R +  L + L+EWQE GF+VT 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 71  SVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEAS 130
           S+CDV +REQREKLMETVSSLF   LNILVNN GT+++K   E T  E S +M+TN +++
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 131 FHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNI 190
           FH  QLAHPLLKASG GSIV +SS++G++ + + + Y A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 191 RANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSV-TPLKRGGEPHEVSSLVAFLCLPAAS 250
           R NA+ PW+I T    P   + + VE MK      TP+ R GE +EVS LVAFLCLPAAS
Sbjct: 183 RTNAICPWLITT----PLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAAS 242

Query: 251 YITGQVICVDGGHTVKAFPF 270
           YITGQVICVDGG TV  F +
Sbjct: 243 YITGQVICVDGGLTVNGFSY 258

[Close]

BLAST of MC05g1489 vs. ExPASy Swiss-Prot
Match: P0DKI3 (Tropinone reductase homolog At1g07440 OS=Arabidopsis thaliana OX=3702 GN=At1g07440 PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.3e-82
Identity = 155/269 (57.62%), Postives = 199/269 (73.98%), Query Frame = 0


Query: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60
           MA AEQ    QRWSLK  T LVTGGT+GIG A VEE A FGAV+HTC+R++ +L++CL +
Sbjct: 1   MAGAEQ---SQRWSLKAKTVLVTGGTKGIGHAIVEEFAGFGAVIHTCARNEYELNECLSK 60

Query: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120
           WQ+ GF+VTGSVCD  +R +REKLM+TVSS+F   L+IL+NN G +  K   + T  + S
Sbjct: 61  WQKKGFQVTGSVCDASLRPEREKLMQTVSSMFGGKLDILINNLGAIRSKPTLDYTAEDFS 120

Query: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN 180
             +STN E+++H  QLAHPLLKASG G+I+F+SS++G++S  + + Y+A+KGA+NQ+ +N
Sbjct: 121 FHISTNLESAYHLSQLAHPLLKASGCGNIIFMSSIAGVVSASVGSIYSATKGALNQLARN 180

Query: 181 LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV 240
           LACEWA D IRANAVAP +I T   E   D+    E  K ++S  PL R GEP EVSSLV
Sbjct: 181 LACEWASDGIRANAVAPAVIATPLAEAVYDD----EFKKVVISRKPLGRFGEPEEVSSLV 240

Query: 241 AFLCLPAASYITGQVICVDGGHTVKAFPF 270
           AFLC+PAASYITGQ ICVDGG TV  F +
Sbjct: 241 AFLCMPAASYITGQTICVDGGLTVNGFSY 262

[Close]

BLAST of MC05g1489 vs. NCBI nr
Match: XP_022145731.1 (tropinone reductase homolog isoform X2 [Momordica charantia])

HSP 1 Score: 542 bits (1396), Expect = 2.58e-194
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0


Query: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60
           MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE
Sbjct: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60

Query: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120
           WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS
Sbjct: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120

Query: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN 180
           RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN
Sbjct: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN 180

Query: 181 LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV 240
           LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV
Sbjct: 181 LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV 240

Query: 241 AFLCLPAASYITGQVICVDGGHTVKAFPFPDL 272
           AFLCLPAASYITGQVICVDGGHTVKAFPFPDL
Sbjct: 241 AFLCLPAASYITGQVICVDGGHTVKAFPFPDL 272

[Close]

BLAST of MC05g1489 vs. NCBI nr
Match: XP_022145730.1 (tropinone reductase homolog isoform X1 [Momordica charantia])

HSP 1 Score: 531 bits (1368), Expect = 8.97e-190
Identity = 272/289 (94.12%), Postives = 272/289 (94.12%), Query Frame = 0


Query: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60
           MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE
Sbjct: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60

Query: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120
           WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS
Sbjct: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120

Query: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKG-------- 180
           RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKG        
Sbjct: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGFFVTKGVS 180

Query: 181 ---------AINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLS 240
                    AINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLS
Sbjct: 181 HKGLFVKEGAINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLS 240

Query: 241 VTPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPDL 272
           VTPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPDL
Sbjct: 241 VTPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPDL 289

[Close]

BLAST of MC05g1489 vs. NCBI nr
Match: XP_022971600.1 (tropinone reductase homolog [Cucurbita maxima])

HSP 1 Score: 447 bits (1151), Expect = 5.05e-157
Identity = 217/264 (82.20%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 8   SRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFE 67
           SR QRWSL+GMTALVTGGT GIGRATVEELAAFGA+VHTCSRSQ DLD+CLKEW+EMG  
Sbjct: 5   SRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEEMGLN 64

Query: 68  VTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNF 127
           V+GSVCDVH REQR++LMETVSSLFN +LNILVNNAGT   KS+ E+TE ELS VMSTNF
Sbjct: 65  VSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAGTSAYKSSLEVTEEELSSVMSTNF 124

Query: 128 EASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAK 187
           EASFHF QLAHPL+KASG GSIVF+SSV+GLMSLP ST YAASK AINQIT+NLACEWAK
Sbjct: 125 EASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLACEWAK 184

Query: 188 DNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPA 247
           DNIR NAVAPWII+TRFV+PP+D+P+HVE M +MLSVTPLKR GEPHEVSS+VAFLCLPA
Sbjct: 185 DNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLCLPA 244

Query: 248 ASYITGQVICVDGGHTVKAFPFPD 271
           ASYITGQV C+DGGHTV AFP PD
Sbjct: 245 ASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of MC05g1489 vs. NCBI nr
Match: XP_022941371.1 (tropinone reductase homolog [Cucurbita moschata])

HSP 1 Score: 446 bits (1148), Expect = 1.45e-156
Identity = 217/264 (82.20%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 8   SRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFE 67
           SR QRWSL+GMTALVTGGT GIGRATVEELAAFGA VHTCSRSQ DLD+CLKEW+EMGF 
Sbjct: 5   SRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFN 64

Query: 68  VTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNF 127
           V+GSVCDVH REQR +L+ETVSSLFN +LNILVNNAGT   KS+ E+TE ELS VMSTNF
Sbjct: 65  VSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAGTSAYKSSLEVTEEELSSVMSTNF 124

Query: 128 EASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAK 187
           EA+FHF QLAHPL+KASG GSIVF+SSV+GLMSLP ST YAASK AINQIT+NLACEWAK
Sbjct: 125 EAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLACEWAK 184

Query: 188 DNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPA 247
           DNIR NAVAPWII+TRFV+PP+D+P+HVE M +MLSVTPLKR GEPHEVSS+VAFLCLPA
Sbjct: 185 DNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLCLPA 244

Query: 248 ASYITGQVICVDGGHTVKAFPFPD 271
           ASYITGQV C+DGGHTVKAFP PD
Sbjct: 245 ASYITGQVFCIDGGHTVKAFPVPD 268

[Close]

BLAST of MC05g1489 vs. NCBI nr
Match: XP_023540048.1 (tropinone reductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 443 bits (1139), Expect = 3.40e-155
Identity = 216/264 (81.82%), Postives = 238/264 (90.15%), Query Frame = 0


Query: 8   SRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFE 67
           SR QRWSL+GMTALVTGGT GIGRATVEELAAFGA VHTCSRSQ DLD+ LKEW+EMGF 
Sbjct: 5   SRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKFLKEWEEMGFN 64

Query: 68  VTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNF 127
           V+GSVCDVH REQR +LMETVSSL+N +LNILVNNAGT   KS+ E+TE ELS VMSTNF
Sbjct: 65  VSGSVCDVHSREQRMQLMETVSSLYNGSLNILVNNAGTSAYKSSLEVTEEELSSVMSTNF 124

Query: 128 EASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAK 187
           EA+FHF QLAHPL+KASG GSIVF+SSV+GLMSLP ST YAASK AINQIT+NLACEWAK
Sbjct: 125 EAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLACEWAK 184

Query: 188 DNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPA 247
           DNIR NAVAPWII+TRFV+PP+D+P+HVE M +MLSVTPLKR GEPHEVSS+VAFLCLPA
Sbjct: 185 DNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLCLPA 244

Query: 248 ASYITGQVICVDGGHTVKAFPFPD 271
           ASYITGQV C+DGGHTVKAFP PD
Sbjct: 245 ASYITGQVFCIDGGHTVKAFPIPD 268

[Close]

BLAST of MC05g1489 vs. ExPASy TrEMBL
Match: A0A6J1CX69 (tropinone reductase homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015115 PE=4 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 1.25e-194
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0


Query: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60
           MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE
Sbjct: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60

Query: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120
           WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS
Sbjct: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120

Query: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN 180
           RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN
Sbjct: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN 180

Query: 181 LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV 240
           LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV
Sbjct: 181 LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV 240

Query: 241 AFLCLPAASYITGQVICVDGGHTVKAFPFPDL 272
           AFLCLPAASYITGQVICVDGGHTVKAFPFPDL
Sbjct: 241 AFLCLPAASYITGQVICVDGGHTVKAFPFPDL 272

[Close]

BLAST of MC05g1489 vs. ExPASy TrEMBL
Match: A0A6J1CVA8 (tropinone reductase homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015115 PE=4 SV=1)

HSP 1 Score: 531 bits (1368), Expect = 4.34e-190
Identity = 272/289 (94.12%), Postives = 272/289 (94.12%), Query Frame = 0


Query: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60
           MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE
Sbjct: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60

Query: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120
           WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS
Sbjct: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120

Query: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKG-------- 180
           RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKG        
Sbjct: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGFFVTKGVS 180

Query: 181 ---------AINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLS 240
                    AINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLS
Sbjct: 181 HKGLFVKEGAINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLS 240

Query: 241 VTPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPDL 272
           VTPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPDL
Sbjct: 241 VTPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPDL 289

[Close]

BLAST of MC05g1489 vs. ExPASy TrEMBL
Match: A0A6J1I667 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=1)

HSP 1 Score: 447 bits (1151), Expect = 2.44e-157
Identity = 217/264 (82.20%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 8   SRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFE 67
           SR QRWSL+GMTALVTGGT GIGRATVEELAAFGA+VHTCSRSQ DLD+CLKEW+EMG  
Sbjct: 5   SRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEEMGLN 64

Query: 68  VTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNF 127
           V+GSVCDVH REQR++LMETVSSLFN +LNILVNNAGT   KS+ E+TE ELS VMSTNF
Sbjct: 65  VSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAGTSAYKSSLEVTEEELSSVMSTNF 124

Query: 128 EASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAK 187
           EASFHF QLAHPL+KASG GSIVF+SSV+GLMSLP ST YAASK AINQIT+NLACEWAK
Sbjct: 125 EASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLACEWAK 184

Query: 188 DNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPA 247
           DNIR NAVAPWII+TRFV+PP+D+P+HVE M +MLSVTPLKR GEPHEVSS+VAFLCLPA
Sbjct: 185 DNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLCLPA 244

Query: 248 ASYITGQVICVDGGHTVKAFPFPD 271
           ASYITGQV C+DGGHTV AFP PD
Sbjct: 245 ASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of MC05g1489 vs. ExPASy TrEMBL
Match: A0A6J1FKX7 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 7.00e-157
Identity = 217/264 (82.20%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 8   SRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFE 67
           SR QRWSL+GMTALVTGGT GIGRATVEELAAFGA VHTCSRSQ DLD+CLKEW+EMGF 
Sbjct: 5   SRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFN 64

Query: 68  VTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNF 127
           V+GSVCDVH REQR +L+ETVSSLFN +LNILVNNAGT   KS+ E+TE ELS VMSTNF
Sbjct: 65  VSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAGTSAYKSSLEVTEEELSSVMSTNF 124

Query: 128 EASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAK 187
           EA+FHF QLAHPL+KASG GSIVF+SSV+GLMSLP ST YAASK AINQIT+NLACEWAK
Sbjct: 125 EAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNLACEWAK 184

Query: 188 DNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPA 247
           DNIR NAVAPWII+TRFV+PP+D+P+HVE M +MLSVTPLKR GEPHEVSS+VAFLCLPA
Sbjct: 185 DNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLCLPA 244

Query: 248 ASYITGQVICVDGGHTVKAFPFPD 271
           ASYITGQV C+DGGHTVKAFP PD
Sbjct: 245 ASYITGQVFCIDGGHTVKAFPVPD 268

[Close]

BLAST of MC05g1489 vs. ExPASy TrEMBL
Match: A0A6J1FN65 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446688 PE=4 SV=1)

HSP 1 Score: 440 bits (1131), Expect = 2.72e-154
Identity = 215/263 (81.75%), Postives = 237/263 (90.11%), Query Frame = 0


Query: 9   RGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEV 68
           R QRWSL+GMTALVTGGT GIGRATVEELAAFGA VHTCSRSQ DLD+CLKEW+EMGF V
Sbjct: 6   RDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNV 65

Query: 69  TGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFE 128
           +GSVCDVH REQR +LMETVSSLFN +LNILVNNAGT   K++ ++TE ELS VMSTNFE
Sbjct: 66  SGSVCDVHSREQRMQLMETVSSLFNGSLNILVNNAGTWAYKNSEDVTEEELSSVMSTNFE 125

Query: 129 ASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKD 188
           ASFHF QLAHPL+KASG GSIVF+SSV+G MSLPLSTAYAASK AINQIT+NLACEWAKD
Sbjct: 126 ASFHFSQLAHPLMKASGNGSIVFMSSVAGSMSLPLSTAYAASKAAINQITRNLACEWAKD 185

Query: 189 NIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPAA 248
           NIR NAVAPWIIKTRFV+PP+D+P+HVE M +MLSVTPLKR GEPHEVSS+VAFLCLP A
Sbjct: 186 NIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLCLPTA 245

Query: 249 SYITGQVICVDGGHTVKAFPFPD 271
           SYITGQV+ VDGGHTV AFP P+
Sbjct: 246 SYITGQVLYVDGGHTVNAFPIPN 268

[Close]

BLAST of MC05g1489 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 1.3e-87
Identity = 162/259 (62.55%), Postives = 195/259 (75.29%), Query Frame = 0


Query: 11  QRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTG 70
           +RWSL G TALVTGGTRGIGRA VEELA FGA VHTCSR+Q++L+ CL +W+  G  V+G
Sbjct: 5   KRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSG 64

Query: 71  SVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEAS 130
           SVCD  +R+QREKL++  SS F+  LNIL+NN GT V K   E +  E +++MSTN E++
Sbjct: 65  SVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESA 124

Query: 131 FHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNI 190
           FH  Q+AHPLLKASG GSIVFISSV+GL+ L   + Y A+KGA+NQ+T+NLACEWA DNI
Sbjct: 125 FHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNI 184

Query: 191 RANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPAASY 250
           R N VAPW IKT  VE   +    VE    ++S TPL R GEP EVSSLVAFLCLPA+SY
Sbjct: 185 RTNCVAPWYIKTSLVETLLEKKEFVEA---VVSRTPLGRVGEPEEVSSLVAFLCLPASSY 244

Query: 251 ITGQVICVDGGHTVKAFPF 270
           ITGQVI VDGG TV  F +
Sbjct: 245 ITGQVISVDGGFTVNGFSY 260

[Close]

BLAST of MC05g1489 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 2.3e-87
Identity = 163/260 (62.69%), Postives = 195/260 (75.00%), Query Frame = 0


Query: 11  QRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTG 70
           +RWSL GM+ALVTGGTRGIGRA VEELA  GA VHTC+R++ +L+ CL +W   GF V G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 71  SVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEAS 130
           SVCDV  R QRE LMETVSS+F   L+ILVNN GT + K   E T  E S +MSTNFE+ 
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESV 183

Query: 131 FHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNI 190
           FH CQLA+PLL+ S  GS+VFISSVSG +SL   +  +++KGAINQ+T++LACEWAKDNI
Sbjct: 184 FHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNI 243

Query: 191 RANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVAFLCLPAASY 250
           R NAVAPW IKT  VE    N  ++E    + SVTPL R GEP EVSS VAFLCLPA+SY
Sbjct: 244 RINAVAPWYIKTSMVEQVLSNKEYLEE---VYSVTPLGRLGEPREVSSAVAFLCLPASSY 303

Query: 251 ITGQVICVDGGHTVKAFPFP 271
           ITGQ++CVDGG ++  F FP
Sbjct: 304 ITGQILCVDGGMSINGF-FP 319

[Close]

BLAST of MC05g1489 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 2.3e-84
Identity = 157/260 (60.38%), Postives = 195/260 (75.00%), Query Frame = 0


Query: 11  QRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTG 70
           +RWSL+GM ALVTGGT+GIG A VEEL+  GA VHTC+R +  L + L+EWQE GF+VT 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 71  SVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEAS 130
           S+CDV +REQREKLMETVSSLF   LNILVNN GT+++K   E T  E S +M+TN +++
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 131 FHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNI 190
           FH  QLAHPLLKASG GSIV +SS++G++ + + + Y A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 191 RANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSV-TPLKRGGEPHEVSSLVAFLCLPAAS 250
           R NA+ PW+I T    P   + + VE MK      TP+ R GE +EVS LVAFLCLPAAS
Sbjct: 183 RTNAICPWLITT----PLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAAS 242

Query: 251 YITGQVICVDGGHTVKAFPF 270
           YITGQVICVDGG TV  F +
Sbjct: 243 YITGQVICVDGGLTVNGFSY 258

[Close]

BLAST of MC05g1489 vs. TAIR 10
Match: AT1G07440.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 8.9e-84
Identity = 155/269 (57.62%), Postives = 199/269 (73.98%), Query Frame = 0


Query: 1   MAEAEQRSRGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKE 60
           MA AEQ    QRWSLK  T LVTGGT+GIG A VEE A FGAV+HTC+R++ +L++CL +
Sbjct: 1   MAGAEQ---SQRWSLKAKTVLVTGGTKGIGHAIVEEFAGFGAVIHTCARNEYELNECLSK 60

Query: 61  WQEMGFEVTGSVCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELS 120
           WQ+ GF+VTGSVCD  +R +REKLM+TVSS+F   L+IL+NN G +  K   + T  + S
Sbjct: 61  WQKKGFQVTGSVCDASLRPEREKLMQTVSSMFGGKLDILINNLGAIRSKPTLDYTAEDFS 120

Query: 121 RVMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKN 180
             +STN E+++H  QLAHPLLKASG G+I+F+SS++G++S  + + Y+A+KGA+NQ+ +N
Sbjct: 121 FHISTNLESAYHLSQLAHPLLKASGCGNIIFMSSIAGVVSASVGSIYSATKGALNQLARN 180

Query: 181 LACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLV 240
           LACEWA D IRANAVAP +I T   E   D+    E  K ++S  PL R GEP EVSSLV
Sbjct: 181 LACEWASDGIRANAVAPAVIATPLAEAVYDD----EFKKVVISRKPLGRFGEPEEVSSLV 240

Query: 241 AFLCLPAASYITGQVICVDGGHTVKAFPF 270
           AFLC+PAASYITGQ ICVDGG TV  F +
Sbjct: 241 AFLCMPAASYITGQTICVDGGLTVNGFSY 262

[Close]

BLAST of MC05g1489 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.2e-82
Identity = 155/260 (59.62%), Postives = 196/260 (75.38%), Query Frame = 0


Query: 12  RWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEVTGS 71
           +WSL+GMTALVTGG++G+G+A VEELA  GA VHTC+R +  L + L+EWQ  G +VT S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 72  VCDVHIREQREKLMETVSSLFNATLNILVNNAGTMVIKSAAEITEAELSRVMSTNFEASF 131
           VCDV  R+QREKLMETVSSLF   L+ILV N G  V+K   E T  E S +++TN E++F
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIATNLESTF 132

Query: 132 HFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNLACEWAKDNIR 191
           HF QLAHPLLKASG G+IV +SSV+G+++L  ++ Y A+KGA+NQ+ +NLACEWA DNIR
Sbjct: 133 HFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNIR 192

Query: 192 ANAVAPWIIKTRFVEPPSDNPVHVETMKYML-SVTPLKRGGEPHEVSSLVAFLCLPAASY 251
           AN+V PW I T    P + + +  + +K  + SVTPL+R GE +EVSSLVAFLCLPAASY
Sbjct: 193 ANSVCPWFITT----PSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASY 252

Query: 252 ITGQVICVDGGHTVKAFPFP 271
           ITGQ ICVDGG T+  F  P
Sbjct: 253 ITGQTICVDGGFTINGFSLP 268

The following BLAST results are available for this feature:

BLAST of MC05g1489 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50165	4.3e-91	62.55	Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
Q9LHT0	1.9e-86	62.55	Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060...	[more]
Q9ZW12	3.2e-86	62.69	Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=...	[more]
Q9ZW13	3.3e-83	60.38	Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292...	[more]
P0DKI3	1.3e-82	57.62	Tropinone reductase homolog At1g07440 OS=Arabidopsis thaliana OX=3702 GN=At1g074...	[more]

BLAST of MC05g1489 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145731.1	2.58e-194	100.00	tropinone reductase homolog isoform X2 [Momordica charantia]	[more]
XP_022145730.1	8.97e-190	94.12	tropinone reductase homolog isoform X1 [Momordica charantia]	[more]
XP_022971600.1	5.05e-157	82.20	tropinone reductase homolog [Cucurbita maxima]	[more]
XP_022941371.1	1.45e-156	82.20	tropinone reductase homolog [Cucurbita moschata]	[more]
XP_023540048.1	3.40e-155	81.82	tropinone reductase homolog [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC05g1489 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CX69	1.25e-194	100.00	tropinone reductase homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CVA8	4.34e-190	94.12	tropinone reductase homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1I667	2.44e-157	82.20	tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=...	[more]
A0A6J1FKX7	7.00e-157	82.20	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 S...	[more]
A0A6J1FN65	2.72e-154	81.75	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446688 PE=4 S...	[more]

BLAST of MC05g1489 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06060.1	1.3e-87	62.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29260.1	2.3e-87	62.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29290.2	2.3e-84	60.38	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G07440.1	8.9e-84	57.62	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	2.2e-82	59.62	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 94..105 score: 48.44 coord: 167..186 score: 38.33 coord: 147..155 score: 53.59
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 188..205 score: 36.69 coord: 226..246 score: 33.82 coord: 167..186 score: 41.85 coord: 94..105 score: 49.49 coord: 141..157 score: 35.2 coord: 19..36 score: 50.2
None	No IPR available	GENE3D	3.40.50.720		coord: 2..267 e-value: 5.2E-82 score: 276.9
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 26..263 e-value: 4.1E-55 score: 186.9
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 21..206 e-value: 1.1E-20 score: 71.8
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 15..266 e-value: 1.4E-11 score: 41.9
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 7..267
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 154..182
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 16..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1489.1	MC05g1489.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity