MC05g1172 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g1172
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
LocationMC05: 15898397 .. 15899647 (+)
RNA-Seq ExpressionMC05g1172
SyntenyMC05g1172
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTCCGTTTCTCTGAAAATCGGAGATGGAACCGCCAGATTCAAGAGGGCCACTCTCTGTTCTTCAGTGCTCAACATTCTCATGCTCATTTCCGTAATCACCACCAATCTCTTTGCAATTTACGCCTTCACTTACTCTCCCAGAGATCGCCAAAATCTCTCGCTTCATCCCGCCCACAAGAACATTTCCCTCATCTCGGAGCAGGTTTCTTTGATTCTCAGAGAGATCGATGTATCGCAGAAGAAGCTTGCTCAGATGGAGAAGGAGATTCTTGGCTATGAGACCATCGACCTCTCCAGATCCAACGTCGCTAACGAGCTCAAACTCTTTCTTCACCGCCACCCGCTCCCTCTCGGCAAGGATTCCAAGAGTGGGATCACTGAAATGGTGGCATCTGTGGGGCATTCGTGTGAGAAATCGATGGACCTTTTGTCGCAGTATATGAATTACAAGGTCTCTGGACCTTGCCCTGACGATTGGAGCCTCGCTCAGAAGCTGATTTTGCGCGGCTGCGAGCCCTTGCCGCGGCGGAGGTGCTTGGCCAAATCTGTGCCCAAGGTAGGTCTGCAACCTTTTCCTGCTTCTCTATGGAAACCTGTTAGCGATAAGCTTGTTATGTGGAGTGGCCTTGGGTGTAAAAATTTCCAGTTTCTGAATAATAAGAAATTGGGCAGGGATTGTGTTGGCTGCTTTGATTTGGTTAGTGGATTTGAGAATCAGAGATTTGTTAAGGCCAGAGGTAAAAATGACTTTCCCATTGATGATGTTTTAGCCTTAACTAGTGGAGGAATCAGGATAGGTTTTGATATTGGAGGAGGGTCTGGGACTTTTGCTGCTAGAATGGCTGAGAAAAATGTCACAGTCATCACATCTACTCTGAACATTGATGCCCCATTCAGTGAATTCATTGCTGCAAGAGGCTTGTTTCCTCTGTTTTTGAGTTTAGATCATAGATTCCCTTTCTATGACAATGTGTTCGATTTGGTTCACGCTTCGACTCGACTGGATATTGGTGGGAAGCCTGAAAAGTTGGAGTTCCTGATGTTCGATATCGACAGGATCTTAAGGGCAGGTGGTTTGCTTTGGTTGGATAACTTCTATTGTGCTAATGATGAGAAGAAAAAATTTTTAACACGTTTGATCGAGCGGTTCGGGTTCAAAAAACTGAAGTGGGTTGTCGGGGAGAAATCTGAGGCGGGAAAATCCGAGGTTTATCTGTCTGCTGTTTTACAGAAGCCAGTAAGAGTA

mRNA sequence

ATGGGGTCCGTTTCTCTGAAAATCGGAGATGGAACCGCCAGATTCAAGAGGGCCACTCTCTGTTCTTCAGTGCTCAACATTCTCATGCTCATTTCCGTAATCACCACCAATCTCTTTGCAATTTACGCCTTCACTTACTCTCCCAGAGATCGCCAAAATCTCTCGCTTCATCCCGCCCACAAGAACATTTCCCTCATCTCGGAGCAGGTTTCTTTGATTCTCAGAGAGATCGATGTATCGCAGAAGAAGCTTGCTCAGATGGAGAAGGAGATTCTTGGCTATGAGACCATCGACCTCTCCAGATCCAACGTCGCTAACGAGCTCAAACTCTTTCTTCACCGCCACCCGCTCCCTCTCGGCAAGGATTCCAAGAGTGGGATCACTGAAATGGTGGCATCTGTGGGGCATTCGTGTGAGAAATCGATGGACCTTTTGTCGCAGTATATGAATTACAAGGTCTCTGGACCTTGCCCTGACGATTGGAGCCTCGCTCAGAAGCTGATTTTGCGCGGCTGCGAGCCCTTGCCGCGGCGGAGGTGCTTGGCCAAATCTGTGCCCAAGGTAGGTCTGCAACCTTTTCCTGCTTCTCTATGGAAACCTGTTAGCGATAAGCTTGTTATGTGGAGTGGCCTTGGGTGTAAAAATTTCCAGTTTCTGAATAATAAGAAATTGGGCAGGGATTGTGTTGGCTGCTTTGATTTGGTTAGTGGATTTGAGAATCAGAGATTTGTTAAGGCCAGAGGTAAAAATGACTTTCCCATTGATGATGTTTTAGCCTTAACTAGTGGAGGAATCAGGATAGGTTTTGATATTGGAGGAGGGTCTGGGACTTTTGCTGCTAGAATGGCTGAGAAAAATGTCACAGTCATCACATCTACTCTGAACATTGATGCCCCATTCAGTGAATTCATTGCTGCAAGAGGCTTGTTTCCTCTGTTTTTGAGTTTAGATCATAGATTCCCTTTCTATGACAATGTGTTCGATTTGGTTCACGCTTCGACTCGACTGGATATTGGTGGGAAGCCTGAAAAGTTGGAGTTCCTGATGTTCGATATCGACAGGATCTTAAGGGCAGGTGGTTTGCTTTGGTTGGATAACTTCTATTGTGCTAATGATGAGAAGAAAAAATTTTTAACACGTTTGATCGAGCGGTTCGGGTTCAAAAAACTGAAGTGGGTTGTCGGGGAGAAATCTGAGGCGGGAAAATCCGAGGTTTATCTGTCTGCTGTTTTACAGAAGCCAGTAAGAGTA

Coding sequence (CDS)

ATGGGGTCCGTTTCTCTGAAAATCGGAGATGGAACCGCCAGATTCAAGAGGGCCACTCTCTGTTCTTCAGTGCTCAACATTCTCATGCTCATTTCCGTAATCACCACCAATCTCTTTGCAATTTACGCCTTCACTTACTCTCCCAGAGATCGCCAAAATCTCTCGCTTCATCCCGCCCACAAGAACATTTCCCTCATCTCGGAGCAGGTTTCTTTGATTCTCAGAGAGATCGATGTATCGCAGAAGAAGCTTGCTCAGATGGAGAAGGAGATTCTTGGCTATGAGACCATCGACCTCTCCAGATCCAACGTCGCTAACGAGCTCAAACTCTTTCTTCACCGCCACCCGCTCCCTCTCGGCAAGGATTCCAAGAGTGGGATCACTGAAATGGTGGCATCTGTGGGGCATTCGTGTGAGAAATCGATGGACCTTTTGTCGCAGTATATGAATTACAAGGTCTCTGGACCTTGCCCTGACGATTGGAGCCTCGCTCAGAAGCTGATTTTGCGCGGCTGCGAGCCCTTGCCGCGGCGGAGGTGCTTGGCCAAATCTGTGCCCAAGGTAGGTCTGCAACCTTTTCCTGCTTCTCTATGGAAACCTGTTAGCGATAAGCTTGTTATGTGGAGTGGCCTTGGGTGTAAAAATTTCCAGTTTCTGAATAATAAGAAATTGGGCAGGGATTGTGTTGGCTGCTTTGATTTGGTTAGTGGATTTGAGAATCAGAGATTTGTTAAGGCCAGAGGTAAAAATGACTTTCCCATTGATGATGTTTTAGCCTTAACTAGTGGAGGAATCAGGATAGGTTTTGATATTGGAGGAGGGTCTGGGACTTTTGCTGCTAGAATGGCTGAGAAAAATGTCACAGTCATCACATCTACTCTGAACATTGATGCCCCATTCAGTGAATTCATTGCTGCAAGAGGCTTGTTTCCTCTGTTTTTGAGTTTAGATCATAGATTCCCTTTCTATGACAATGTGTTCGATTTGGTTCACGCTTCGACTCGACTGGATATTGGTGGGAAGCCTGAAAAGTTGGAGTTCCTGATGTTCGATATCGACAGGATCTTAAGGGCAGGTGGTTTGCTTTGGTTGGATAACTTCTATTGTGCTAATGATGAGAAGAAAAAATTTTTAACACGTTTGATCGAGCGGTTCGGGTTCAAAAAACTGAAGTGGGTTGTCGGGGAGAAATCTGAGGCGGGAAAATCCGAGGTTTATCTGTCTGCTGTTTTACAGAAGCCAGTAAGAGTA

Protein sequence

MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAHKNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLGKDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRCLAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFENQRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPFSEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGGLLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV
Homology
BLAST of MC05g1172 vs. ExPASy Swiss-Prot
Match: Q8RWB7 (Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.1e-56
Identity = 134/396 (33.84%), Postives = 210/396 (53.03%), Query Frame = 0

Query: 25  LNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAHKNISLISEQVSLILREIDVSQKKL 84
           LN+L+L++++ TN+ ++Y  + +    Q+ ++  +  ++  + + +   L  I  +   L
Sbjct: 8   LNLLLLVAMVATNILSLYHLSSTTNFFQS-TVKSSQSSVPTVPDHLLRQLHTIRAAINHL 67

Query: 85  AQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLGKDSKSGITEMVASVGHSCEKSMDL 144
              + +     +   SR+ V++       +  L         I   ++ +  +C    DL
Sbjct: 68  TTHQPD--KSTSTSTSRAAVSSSSSSTAPKELL---------IYSKLSPIASACHNYPDL 127

Query: 145 LSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRCLAKSVPKVGLQPFPASLWKPVSDK 204
           L +YMNY     CP D  L +KLILRGC PLPRRRC +++       P   S  KP S+ 
Sbjct: 128 LHEYMNYTPFSLCPSDTDLVEKLILRGCHPLPRRRCFSRT-------PRNPSDSKPESN- 187

Query: 205 LVMWSGLGCKNFQFLNNK--KLGRDCVGCFDLVSGFENQRFVKARGKNDFPIDDVLALTS 264
            V+WS   CK+F  L  K   LG      FDL       +F   + + D PI  +L +  
Sbjct: 188 -VLWSYYSCKSFDCLITKFSDLG------FDLSLEKSKSQFSAYKSELDLPISQLLQIAK 247

Query: 265 GG---IRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPFSEFIAARGLFPLFLSLDHR 324
                +R+G D+GGG+G+FAA M  +NVTV+T+T+N +AP+SE +A RGL PL + L  R
Sbjct: 248 SANSVLRLGIDVGGGTGSFAAAMKARNVTVLTTTMNFNAPYSEAVAMRGLVPLHVPLQQR 307

Query: 325 FPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGGLLWLDNFYCANDEKKKFLT 384
            P +D V DLV     ++       +EF  FD+DRILR GG LWLD F+    + +    
Sbjct: 308 LPVFDGVVDLVRCGRAVNRWIPVTVMEFFFFDLDRILRGGGYLWLDRFFSKKVDLENVYA 367

Query: 385 RLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPV 416
            +I + G+KK+KW V  K+++   EV+L+A+LQKPV
Sbjct: 368 PMIGKLGYKKVKWAVANKADSKHGEVFLTALLQKPV 376

BLAST of MC05g1172 vs. ExPASy Swiss-Prot
Match: O80844 (Probable methyltransferase PMT16 OS=Arabidopsis thaliana OX=3702 GN=At2g45750 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.9e-05
Identity = 39/134 (29.10%), Postives = 62/134 (46.27%), Query Frame = 0

Query: 238 FENQRFVKARGKNDFP------IDDV---LALTSGGIRIGFDIGGGSGTFAARMAEKNVT 297
           +EN RF    G   FP      IDD+   + L+ G IR   D G G  +F A +  +N+T
Sbjct: 173 YENDRFWFPGGGTMFPRGADAYIDDIGRLIDLSDGSIRTAIDTGCGVASFGAYLLSRNIT 232

Query: 298 VIT-STLNIDAPFSEFIAARGLFPLF-LSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLE 357
            ++ +  +      +F   RG+  +  +    R P+    FDL H S  L   G+ +   
Sbjct: 233 TMSFAPRDTHEAQVQFALERGVPAMIGIMATIRLPYPSRAFDLAHCSRCLIPWGQNDGA- 292

Query: 358 FLMFDIDRILRAGG 361
             + ++DR+LR GG
Sbjct: 293 -YLMEVDRVLRPGG 304

BLAST of MC05g1172 vs. ExPASy Swiss-Prot
Match: Q8H118 (Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=2 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 1.2e-04
Identity = 42/146 (28.77%), Postives = 63/146 (43.15%), Query Frame = 0

Query: 260 LTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPFSEFIAARGLFPLFLSLDHR 319
           + S  +R   D+    G+FAA + EK+V V+           + I  RGL     S    
Sbjct: 450 IQSDTVRNIMDMKASMGSFAAALKEKDVWVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEA 509

Query: 320 FPFYDNVFDLVHASTRL-DIGGKPEKLEFLMFDIDRILRAGGLLWLDNFYCANDEKKKFL 379
           F  Y   +DL+HA   + DI  +    E L+ ++DRILR  G + + +     D  KK+L
Sbjct: 510 FSTYPRTYDLLHAWDIISDIKKRGCSAEDLLLEMDRILRPSGFILIRDKQSVVDLVKKYL 569

Query: 380 TRLIERFGFKKLKWVVGEKSEAGKSE 405
                    K L W   E   A +S+
Sbjct: 570 ---------KALHWEAVETKTASESD 586

BLAST of MC05g1172 vs. ExPASy Swiss-Prot
Match: Q9FG39 (Probable methyltransferase PMT12 OS=Arabidopsis thaliana OX=3702 GN=At5g06050 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.6e-04
Identity = 32/103 (31.07%), Postives = 51/103 (49.51%), Query Frame = 0

Query: 264 GIRIGFDIGGGSGTFAARMAEKNVTV-ITSTLNIDAPFS-EFIAARGLFPLFLSLDHRFP 323
           G+R   D+  G G FAA +AE  V   + + + +  P +   I  RGL  +       F 
Sbjct: 518 GLRNVLDMRAGFGGFAAALAELKVDCWVLNVIPVSGPNTLPVIYDRGLLGVMHDWCEPFD 577

Query: 324 FYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGGLLWL 365
            Y   +DL+HA+    I  K   +  +M ++DRILR GG +++
Sbjct: 578 TYPRTYDLLHAAGLFSIERKRCNMTTMMLEMDRILRPGGRVYI 620

BLAST of MC05g1172 vs. NCBI nr
Match: XP_022151416.1 (uncharacterized protein LOC111019353 [Momordica charantia])

HSP 1 Score: 833 bits (2153), Expect = 8.43e-305
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN
Sbjct: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. NCBI nr
Match: XP_004136545.1 (uncharacterized protein LOC101215265 [Cucumis sativus] >KGN59257.1 hypothetical protein Csa_001674 [Cucumis sativus])

HSP 1 Score: 790 bits (2041), Expect = 9.92e-288
Identity = 393/417 (94.24%), Postives = 406/417 (97.36%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSS LNILMLISV+TTNLFA+YAFTYSP+DR+  +L+  H
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSALNILMLISVVTTNLFALYAFTYSPKDREIHTLNRTH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYE+IDLSRSNVANELKLFL RHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYESIDLSRSNVANELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP SLWKPVSDK+VMWSGLGCKNFQ LN+KKLGRDCVGCFDL++GFEN
Sbjct: 181 LAKSVPKVGLQPFPISLWKPVSDKIVMWSGLGCKNFQCLNSKKLGRDCVGCFDLINGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS  LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASNGLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFGFKKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGFKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. NCBI nr
Match: XP_038906243.1 (uncharacterized protein LOC120092107 [Benincasa hispida])

HSP 1 Score: 789 bits (2037), Expect = 4.04e-287
Identity = 394/417 (94.48%), Postives = 406/417 (97.36%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSS LNILMLISVITTNLFA+YAFTYSP+DR+  SL+  H
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSALNILMLISVITTNLFALYAFTYSPKDREIHSLNRTH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYE+IDLSRS+VANELKLFL RHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYESIDLSRSSVANELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP SLW PVSDK+VMWSGLGCKNFQ LN+KKLGRDCVGCFDLV+GFEN
Sbjct: 181 LAKSVPKVGLQPFPKSLWNPVSDKIVMWSGLGCKNFQCLNSKKLGRDCVGCFDLVNGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS+ LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASSGLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFGFKKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGFKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. NCBI nr
Match: XP_008442993.1 (PREDICTED: uncharacterized protein LOC103486724 [Cucumis melo] >KAA0043768.1 Methyltransf_29 domain-containing protein [Cucumis melo var. makuwa] >TYK25366.1 Methyltransf_29 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 788 bits (2035), Expect = 8.15e-287
Identity = 392/417 (94.00%), Postives = 405/417 (97.12%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSS LNILMLISV+TTNLFA+YAFTYSP+DR+  +L   H
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSALNILMLISVVTTNLFALYAFTYSPKDREIHTLDRTH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYE+IDLSRSNVANELKLFL RHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYESIDLSRSNVANELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP SLWKPVSDK+VMWSGLGCKNFQ LN+KKLGRDCVGCFDL++GFEN
Sbjct: 181 LAKSVPKVGLQPFPISLWKPVSDKIVMWSGLGCKNFQCLNSKKLGRDCVGCFDLINGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLAL+SGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALSSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS  LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASNGLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFGFKKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGFKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. NCBI nr
Match: XP_022982960.1 (uncharacterized protein LOC111481651 [Cucurbita maxima])

HSP 1 Score: 780 bits (2013), Expect = 1.83e-283
Identity = 388/417 (93.05%), Postives = 405/417 (97.12%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRA++CSS LNILMLISVITTNLFA+YAFTYSP+DR+  SL+ AH
Sbjct: 1   MGSVSLKIGDGTARFKRASICSSALNILMLISVITTNLFALYAFTYSPKDREVHSLNRAH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KN S+ISEQVSLILREI+VSQKKLAQMEKEILGY++IDLSRSNVA+ELKLFL RHPLPLG
Sbjct: 61  KNFSVISEQVSLILREIEVSQKKLAQMEKEILGYQSIDLSRSNVADELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP+SLWKPVSDK+VMWSGLGCKNF  LN+KKLGRDCVGCFDLVSGFEN
Sbjct: 181 LAKSVPKVGLQPFPSSLWKPVSDKIVMWSGLGCKNFNCLNSKKLGRDCVGCFDLVSGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDF IDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFSIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS+ LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASSALDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFG KKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGLKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. ExPASy TrEMBL
Match: A0A6J1DEK9 (uncharacterized protein LOC111019353 OS=Momordica charantia OX=3673 GN=LOC111019353 PE=3 SV=1)

HSP 1 Score: 833 bits (2153), Expect = 4.08e-305
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN
Sbjct: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. ExPASy TrEMBL
Match: A0A0A0LEX5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G791480 PE=3 SV=1)

HSP 1 Score: 790 bits (2041), Expect = 4.80e-288
Identity = 393/417 (94.24%), Postives = 406/417 (97.36%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSS LNILMLISV+TTNLFA+YAFTYSP+DR+  +L+  H
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSALNILMLISVVTTNLFALYAFTYSPKDREIHTLNRTH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYE+IDLSRSNVANELKLFL RHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYESIDLSRSNVANELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP SLWKPVSDK+VMWSGLGCKNFQ LN+KKLGRDCVGCFDL++GFEN
Sbjct: 181 LAKSVPKVGLQPFPISLWKPVSDKIVMWSGLGCKNFQCLNSKKLGRDCVGCFDLINGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS  LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASNGLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFGFKKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGFKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. ExPASy TrEMBL
Match: A0A5A7TLP5 (Methyltransf_29 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005050 PE=3 SV=1)

HSP 1 Score: 788 bits (2035), Expect = 3.94e-287
Identity = 392/417 (94.00%), Postives = 405/417 (97.12%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSS LNILMLISV+TTNLFA+YAFTYSP+DR+  +L   H
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSALNILMLISVVTTNLFALYAFTYSPKDREIHTLDRTH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYE+IDLSRSNVANELKLFL RHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYESIDLSRSNVANELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP SLWKPVSDK+VMWSGLGCKNFQ LN+KKLGRDCVGCFDL++GFEN
Sbjct: 181 LAKSVPKVGLQPFPISLWKPVSDKIVMWSGLGCKNFQCLNSKKLGRDCVGCFDLINGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLAL+SGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALSSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS  LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASNGLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFGFKKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGFKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. ExPASy TrEMBL
Match: A0A1S3B731 (uncharacterized protein LOC103486724 OS=Cucumis melo OX=3656 GN=LOC103486724 PE=3 SV=1)

HSP 1 Score: 788 bits (2035), Expect = 3.94e-287
Identity = 392/417 (94.00%), Postives = 405/417 (97.12%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRATLCSS LNILMLISV+TTNLFA+YAFTYSP+DR+  +L   H
Sbjct: 1   MGSVSLKIGDGTARFKRATLCSSALNILMLISVVTTNLFALYAFTYSPKDREIHTLDRTH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KNISLISEQVSLILREIDVSQKKLAQMEKEILGYE+IDLSRSNVANELKLFL RHPLPLG
Sbjct: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYESIDLSRSNVANELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP SLWKPVSDK+VMWSGLGCKNFQ LN+KKLGRDCVGCFDL++GFEN
Sbjct: 181 LAKSVPKVGLQPFPISLWKPVSDKIVMWSGLGCKNFQCLNSKKLGRDCVGCFDLINGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDFPIDDVLAL+SGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFPIDDVLALSSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS  LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASNGLDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFGFKKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGFKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. ExPASy TrEMBL
Match: A0A6J1J4B7 (uncharacterized protein LOC111481651 OS=Cucurbita maxima OX=3661 GN=LOC111481651 PE=3 SV=1)

HSP 1 Score: 780 bits (2013), Expect = 8.88e-284
Identity = 388/417 (93.05%), Postives = 405/417 (97.12%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKRA++CSS LNILMLISVITTNLFA+YAFTYSP+DR+  SL+ AH
Sbjct: 1   MGSVSLKIGDGTARFKRASICSSALNILMLISVITTNLFALYAFTYSPKDREVHSLNRAH 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
           KN S+ISEQVSLILREI+VSQKKLAQMEKEILGY++IDLSRSNVA+ELKLFL RHPLPLG
Sbjct: 61  KNFSVISEQVSLILREIEVSQKKLAQMEKEILGYQSIDLSRSNVADELKLFLQRHPLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC
Sbjct: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAKSVPKVGLQPFP+SLWKPVSDK+VMWSGLGCKNF  LN+KKLGRDCVGCFDLVSGFEN
Sbjct: 181 LAKSVPKVGLQPFPSSLWKPVSDKIVMWSGLGCKNFNCLNSKKLGRDCVGCFDLVSGFEN 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
           QRFVKARGKNDF IDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF
Sbjct: 241 QRFVKARGKNDFSIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHAS+ LDIGGKPEKLEFLMFDIDRILRAGG
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASSALDIGGKPEKLEFLMFDIDRILRAGG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 417
           LLWLDNFYCANDEKKK LTRLIERFG KKLKWV+GEKSE+GKSEVYLSAVLQKPVRV
Sbjct: 361 LLWLDNFYCANDEKKKALTRLIERFGLKKLKWVIGEKSESGKSEVYLSAVLQKPVRV 417

BLAST of MC05g1172 vs. TAIR 10
Match: AT3G27230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 609.0 bits (1569), Expect = 2.9e-174
Identity = 306/416 (73.56%), Postives = 348/416 (83.65%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARF+R ++CSS +N+LML SV+TTNLFA+YAF  S   + N  L  + 
Sbjct: 1   MGSVSLKIGDGTARFRRTSICSSAVNLLMLFSVVTTNLFALYAF--SSHSQANSPLLHSS 60

Query: 61  KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPLG 120
            NISL+S+ +SLILREID SQ+KLAQMEK++LGYE+ID+SR N+  ELKLFL RH LPLG
Sbjct: 61  NNISLVSQHLSLILREIDSSQRKLAQMEKQMLGYESIDISRPNIVPELKLFLQRHQLPLG 120

Query: 121 KDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRRC 180
           KDS++GITEMV+SVGHSC KS DLLSQYM+YKV   CPDDWSL QKLILR CEPLPRRRC
Sbjct: 121 KDSRTGITEMVSSVGHSCGKSTDLLSQYMSYKVFDRCPDDWSLGQKLILRACEPLPRRRC 180

Query: 181 LAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFEN 240
           LAK+V K  L   P SLW+ VS+K V WSGLGCK+F  L  KKL ++CVGCFDL  G E 
Sbjct: 181 LAKTVQKQDLSKSPDSLWRSVSNKSVNWSGLGCKSFDCLKGKKLSKECVGCFDL--GVEK 240

Query: 241 QRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAPF 300
            RFVK +GKNDF IDDVL L SG IRIGFDI GGSGTFAARMAEKNVTVIT+TLN  APF
Sbjct: 241 DRFVKVKGKNDFLIDDVLGLGSGKIRIGFDISGGSGTFAARMAEKNVTVITNTLNNGAPF 300

Query: 301 SEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGKPEKLEFLMFDIDRILRAGG 360
           SEFIAARGLFPLFLSLDHRFPF DNVFDL+HAS+ LD+ GK EKLEFLMFD+DR+L+  G
Sbjct: 301 SEFIAARGLFPLFLSLDHRFPFLDNVFDLIHASSGLDVEGKAEKLEFLMFDLDRVLKPRG 360

Query: 361 LLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVR 417
           L WLDNFYCANDEKKK LTR+IERFG+KKLKWV+GEK++A   +VYLSAVLQKPVR
Sbjct: 361 LFWLDNFYCANDEKKKELTRMIERFGYKKLKWVIGEKADA---QVYLSAVLQKPVR 409

BLAST of MC05g1172 vs. TAIR 10
Match: AT5G40830.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 598.2 bits (1541), Expect = 5.1e-171
Identity = 298/420 (70.95%), Postives = 343/420 (81.67%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKR+TL SS +N+LML S++TTNLFA+YAF+      Q+ + HP H
Sbjct: 1   MGSVSLKIGDGTARFKRSTLFSSAINLLMLFSIVTTNLFALYAFS---SRSQSHTPHPLH 60

Query: 61  -KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPL 120
             N+SL+S+ +SLILREID S   L QMEK+I+GYE++DLS+  V  ELKLFL +H LPL
Sbjct: 61  SNNVSLVSQHLSLILREIDSSHHTLTQMEKQIIGYESLDLSQQEVPQELKLFLQQHQLPL 120

Query: 121 GKDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRR 180
           GKDS++GIT+MVASVGHSCE S+DLLSQYM+Y V   CPDDWSLAQKLILR CEPLPRRR
Sbjct: 121 GKDSRTGITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRR 180

Query: 181 CLAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFE 240
           CLAK+V K GL  FP SLW+PV +  V WSGLGCK+F+ L  KKL RDCVGCFDL +  E
Sbjct: 181 CLAKTVHKPGLALFPDSLWRPVGNSSVNWSGLGCKSFECLKGKKLSRDCVGCFDLATSHE 240

Query: 241 NQRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAP 300
             RFVK  GK DF IDDVL L  G IRIGFDI  GSGTFAARMAEKNV +I++TLNIDAP
Sbjct: 241 KDRFVKVNGKTDFLIDDVLDLGDGKIRIGFDISSGSGTFAARMAEKNVNIISNTLNIDAP 300

Query: 301 FSEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLD--IGGKPEKLEFLMFDIDRILR 360
           FSEFIAARG+FPLF+SLD R PFYDNVFDL+HAS  LD  +  KPEKLEFLMFD+DRIL+
Sbjct: 301 FSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDLDRILK 360

Query: 361 AGGLLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 418
            GGL WLDNFYC NDEKK+ LTRLIERFG+KKLKWVVGEK++A   EV+LSAVLQKP R+
Sbjct: 361 PGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEKTDA---EVFLSAVLQKPARI 414

BLAST of MC05g1172 vs. TAIR 10
Match: AT5G40830.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 598.2 bits (1541), Expect = 5.1e-171
Identity = 298/420 (70.95%), Postives = 343/420 (81.67%), Query Frame = 0

Query: 1   MGSVSLKIGDGTARFKRATLCSSVLNILMLISVITTNLFAIYAFTYSPRDRQNLSLHPAH 60
           MGSVSLKIGDGTARFKR+TL SS +N+LML S++TTNLFA+YAF+      Q+ + HP H
Sbjct: 1   MGSVSLKIGDGTARFKRSTLFSSAINLLMLFSIVTTNLFALYAFS---SRSQSHTPHPLH 60

Query: 61  -KNISLISEQVSLILREIDVSQKKLAQMEKEILGYETIDLSRSNVANELKLFLHRHPLPL 120
             N+SL+S+ +SLILREID S   L QMEK+I+GYE++DLS+  V  ELKLFL +H LPL
Sbjct: 61  SNNVSLVSQHLSLILREIDSSHHTLTQMEKQIIGYESLDLSQQEVPQELKLFLQQHQLPL 120

Query: 121 GKDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQKLILRGCEPLPRRR 180
           GKDS++GIT+MVASVGHSCE S+DLLSQYM+Y V   CPDDWSLAQKLILR CEPLPRRR
Sbjct: 121 GKDSRTGITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRR 180

Query: 181 CLAKSVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLGRDCVGCFDLVSGFE 240
           CLAK+V K GL  FP SLW+PV +  V WSGLGCK+F+ L  KKL RDCVGCFDL +  E
Sbjct: 181 CLAKTVHKPGLALFPDSLWRPVGNSSVNWSGLGCKSFECLKGKKLSRDCVGCFDLATSHE 240

Query: 241 NQRFVKARGKNDFPIDDVLALTSGGIRIGFDIGGGSGTFAARMAEKNVTVITSTLNIDAP 300
             RFVK  GK DF IDDVL L  G IRIGFDI  GSGTFAARMAEKNV +I++TLNIDAP
Sbjct: 241 KDRFVKVNGKTDFLIDDVLDLGDGKIRIGFDISSGSGTFAARMAEKNVNIISNTLNIDAP 300

Query: 301 FSEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLD--IGGKPEKLEFLMFDIDRILR 360
           FSEFIAARG+FPLF+SLD R PFYDNVFDL+HAS  LD  +  KPEKLEFLMFD+DRIL+
Sbjct: 301 FSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDLDRILK 360

Query: 361 AGGLLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAGKSEVYLSAVLQKPVRV 418
            GGL WLDNFYC NDEKK+ LTRLIERFG+KKLKWVVGEK++A   EV+LSAVLQKP R+
Sbjct: 361 PGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEKTDA---EVFLSAVLQKPARI 414

BLAST of MC05g1172 vs. TAIR 10
Match: AT3G05390.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF248, methyltransferase putative (InterPro:IPR004159); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G01240.1); Has 507 Blast hits to 498 proteins in 33 species: Archae - 4; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 493; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 260.4 bits (664), Expect = 2.5e-69
Identity = 135/315 (42.86%), Postives = 193/315 (61.27%), Query Frame = 0

Query: 107 ELKLFLHRHPLPLGKDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQK 166
           E++ ++      +GK +  G   +  ++GH+C      L +YM+Y V   C DDW+LAQK
Sbjct: 153 EIRQYITPKENRVGKINMFGTERVYNTIGHACALMKMELEKYMDYDVGAYCDDDWNLAQK 212

Query: 167 LILRGCEPLPRRRCLAK-SVPKVGLQPFPASLWKPVSDKLVMWSGLGCKNFQFLNNKKLG 226
           L+L GC+PLPRRRCL + S+      P   SLWK   D+ V W    C+NF  L++K   
Sbjct: 213 LMLNGCDPLPRRRCLTRASMTYQKPYPINESLWKLPDDRNVRWGNYQCRNFACLSSKNPK 272

Query: 227 R---DCVGCFDLVSGFENQRFVKARG-KNDFPIDDVLALTSGGIRIGFDIGGGSGTFAAR 286
           R    C GCF++    E  ++VK      DF I+DVL +    IRIG D G G+GTFAAR
Sbjct: 273 RGYTKCSGCFEMEK--EKDKWVKNSSLLVDFMIEDVLRVKPSEIRIGLDYGVGTGTFAAR 332

Query: 287 MAEKNVTVITSTLNIDAPFSEFIAARGLFPLFLSLDHRFPFYDNVFDLVHASTRLDIGGK 346
           M EKNVT++T+ LN+ APF+E IA RGL PL++SL+ R PF+DN  D++H +  +D    
Sbjct: 333 MREKNVTIVTTALNLGAPFNEMIALRGLIPLYISLNQRLPFFDNTMDMIHTTGLMDGWID 392

Query: 347 PEKLEFLMFDIDRILRAGGLLWLDNFYCANDEKKKFLTRLIERFGFKKLKWVVGEKSEAG 406
              ++F+++D DR+LR GGLLW+D F+C   +   ++   ++ F +KK KW +  KS   
Sbjct: 393 LLLMDFVLYDWDRVLRPGGLLWIDRFFCKKKDLDDYMYMFLQ-FRYKKHKWAISPKS--- 452

Query: 407 KSEVYLSAVLQKPVR 417
           K EVYLSA+L+KP R
Sbjct: 453 KDEVYLSALLEKPPR 461

BLAST of MC05g1172 vs. TAIR 10
Match: AT4G01240.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 6.8e-67
Identity = 134/324 (41.36%), Postives = 195/324 (60.19%), Query Frame = 0

Query: 107 ELKLFLHRHPLPLGKDSKSGITEMVASVGHSCEKSMDLLSQYMNYKVSGPCPDDWSLAQK 166
           E+K ++   P  LGK +  G      S+GH+C      L +YM+Y V   C DDW LAQK
Sbjct: 337 EIKKYIKIKPNRLGKQNFMGANGTFTSIGHACFAMKKDLEEYMDYDVGEICNDDWRLAQK 396

Query: 167 LILRGCEPLPRRRCLAKSVPKVGLQPFP--ASLWKPVSDKLVMWSGLGCKNFQFLNNKKL 226
           L++ GC+PLPRRRC ++  P++  +PFP   SLWK   ++ V W    CKNF  L +   
Sbjct: 397 LMVHGCDPLPRRRCFSRG-PQLYHKPFPINESLWKLPDNRNVRWGQYKCKNFACLASNTT 456

Query: 227 GR----DCVGCFDLVSGFENQRFVKARGK--------NDFPIDDVLALTSGGIRIGFDIG 286
            R     C  CF+L +  E+ R++  RG+         DF I +VL +  G IRIG D  
Sbjct: 457 ARKGFFKCTDCFNL-THHESPRWLN-RGEIDPETNQTADFSIAEVLEIKPGEIRIGLDFS 516

Query: 287 GGSGTFAARMAEKNVTVITSTLNIDAPFSEFIAARGLFPLFLSLDHRFPFYDNVFDLVHA 346
            G+GTFAARM E+NVT++++T+N+ APF+E IA RGL PL+L+++ R PF+D+  D++H 
Sbjct: 517 IGTGTFAARMREQNVTIVSATINLGAPFNEMIALRGLVPLYLTVNQRLPFFDSTLDMIHT 576

Query: 347 STRLDIGGKPEKLEFLMFDIDRILRAGGLLWLDNFYCANDEKKKFLTRLIERFGFKKLKW 406
           +  LD       L+F++FD DR+LR GGLLW+D F+C  ++   ++    +   ++K KW
Sbjct: 577 TRFLDGWIDLILLDFVLFDWDRVLRPGGLLWIDGFFCLKEDVSDYM-EAFKALRYRKHKW 636

Query: 407 VVGEKSEAGKSEVYLSAVLQKPVR 417
           VV  K +    EV+ SAVL+KP R
Sbjct: 637 VVVPKKDKDDKEVFFSAVLEKPPR 656

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWB71.1e-5633.84Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE... [more]
O808441.9e-0529.10Probable methyltransferase PMT16 OS=Arabidopsis thaliana OX=3702 GN=At2g45750 PE... [more]
Q8H1181.2e-0428.77Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=... [more]
Q9FG393.6e-0431.07Probable methyltransferase PMT12 OS=Arabidopsis thaliana OX=3702 GN=At5g06050 PE... [more]
Match NameE-valueIdentityDescription
XP_022151416.18.43e-305100.00uncharacterized protein LOC111019353 [Momordica charantia][more]
XP_004136545.19.92e-28894.24uncharacterized protein LOC101215265 [Cucumis sativus] >KGN59257.1 hypothetical ... [more]
XP_038906243.14.04e-28794.48uncharacterized protein LOC120092107 [Benincasa hispida][more]
XP_008442993.18.15e-28794.00PREDICTED: uncharacterized protein LOC103486724 [Cucumis melo] >KAA0043768.1 Met... [more]
XP_022982960.11.83e-28393.05uncharacterized protein LOC111481651 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1DEK94.08e-305100.00uncharacterized protein LOC111019353 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A0A0LEX54.80e-28894.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G791480 PE=3 SV=1[more]
A0A5A7TLP53.94e-28794.00Methyltransf_29 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3B7313.94e-28794.00uncharacterized protein LOC103486724 OS=Cucumis melo OX=3656 GN=LOC103486724 PE=... [more]
A0A6J1J4B78.88e-28493.05uncharacterized protein LOC111481651 OS=Cucurbita maxima OX=3661 GN=LOC111481651... [more]
Match NameE-valueIdentityDescription
AT3G27230.12.9e-17473.56S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.15.1e-17170.95S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.25.1e-17170.95S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G05390.12.5e-6942.86FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT4G01240.16.8e-6741.36S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 70..90
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 239..405
e-value: 2.2E-8
score: 35.7
NoneNo IPR availablePANTHERPTHR44067S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN-RELATEDcoord: 1..417
NoneNo IPR availablePANTHERPTHR44067:SF3S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEINcoord: 1..417
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 263..364
e-value: 1.5E-6
score: 27.0
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 269..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g1172.1MC05g1172.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity