MC05g1162 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g1162
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionF-box/kelch-repeat protein
LocationMC05: 15806964 .. 15807983 (+)
RNA-Seq ExpressionMC05g1162
SyntenyMC05g1162
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTTGTGGCCGGTGGCCACGACCAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAAATATGGGTGGTTAGCGGCTACGGGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGTGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGATGCGGCGGCGGCGACGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

mRNA sequence

GAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTTGTGGCCGGTGGCCACGACCAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAAATATGGGTGGTTAGCGGCTACGGGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGTGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGATGCGGCGGCGGCGACGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

Coding sequence (CDS)

GAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTTGTGGCCGGTGGCCACGACCAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAAATATGGGTGGTTAGCGGCTACGGGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGTGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGATGCGGCGGCGGCGACGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

Protein sequence

ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKTGTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAKEGMVGVELGERAMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI
Homology
BLAST of MC05g1162 vs. ExPASy Swiss-Prot
Match: O80582 (F-box/kelch-repeat protein At2g44130 OS=Arabidopsis thaliana OX=3702 GN=At2g44130 PE=2 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 5.1e-49
Identity = 109/283 (38.52%), Postives = 154/283 (54.42%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+P E+ALECL R  +        VCR WR L     F + R + G+T   + L+Q
Sbjct: 18  ELIPGLPSELALECLVRVPFQFQSAMRSVCRSWRSLLSDSSFIQERRRCGKTELLLCLVQ 77

Query: 61  SLSAQAGP----------DGAKAATSAN-------AYGVSVFDPAAGNWTRIEPIGKYP- 120
            L+               D  K+   ++        +G+SV++ A   W R+     +P 
Sbjct: 78  PLTPPIPASKSVDETLMVDEKKSEDESHPRVFCTPRFGLSVYNAAMSTWHRV----AFPE 137

Query: 121 -NGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFG 180
              +PLFC   ++   GK+++IGGWDP T +P  DV+V EFA  +WR+G  M E RSFF 
Sbjct: 138 EEQIPLFCECVVLQDAGKILLIGGWDPETLQPTRDVYVLEFAGRKWRRGAPMKESRSFFA 197

Query: 181 -AAAHGGEIFVAGGHDQGKNAAATAWAYDVRQDEWRELPAMGAARDECE--AVAIGSEIW 240
            A+    +++VAGGHD  KNA  +A  YDV +DEW  +  M   RDEC+  AV +G    
Sbjct: 198 CASVSPTKVYVAGGHDDQKNALRSAEVYDVEKDEWSSVTPMTEGRDECQGFAVGMGLRFC 257

Query: 241 VVSGYGTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERCPRG 259
           V+SGYGTE+QG F    E++D  T +W R+D  W+  +  PRG
Sbjct: 258 VLSGYGTESQGRFRSDGEIYDPATDSWSRIDNVWRFPDTSPRG 296

BLAST of MC05g1162 vs. ExPASy Swiss-Prot
Match: Q9LMR5 (F-box/kelch-repeat protein At1g15670 OS=Arabidopsis thaliana OX=3702 GN=At1g15670 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-48
Identity = 123/363 (33.88%), Postives = 172/363 (47.38%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIP +PE +A ECL RS Y    + A VC+ W++     DF++ R  SG + + V L  
Sbjct: 2   ELIPDLPETVAYECLLRSSYKQFPLMASVCKLWQREISLSDFFRHRKASGHSQELVVL-- 61

Query: 61  SLSAQAGPDGAKAATSAN------AYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISS 120
              +QA  D  K   S N       Y +SV +   G  + + P+  + NGLPLFCR++S 
Sbjct: 62  ---SQARVDPVKELVSGNKTIPTPVYRISVLELGTGLRSELPPVPGHSNGLPLFCRLVSV 121

Query: 121 KGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHG-GEIFVAGG 180
              LVV+ G DP T+R  + VFV+ F T  WR GK+MP   RSFF  A+     +FVAGG
Sbjct: 122 GSDLVVLCGLDPVTWRTSDSVFVFSFLTSTWRVGKSMPGGPRSFFACASDSQRNVFVAGG 181

Query: 181 HDQGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDS 240
           HD+ KNA  +A  YDV +D W  LP MG  RDEC A+    +  V+ GY TE QG F  +
Sbjct: 182 HDEDKNAMMSALVYDVAEDRWAFLPDMGRERDECTAIFHAGKFHVIGGYSTEEQGQFSKT 241

Query: 241 AEVFDTKTGTWRRVDGAWKGERCPR--GVVGVRGGGGIY-------------NWAEADAA 300
           AE FD  T  W      +          +      G +Y              W +    
Sbjct: 242 AESFDVTTWRWSPQGEEFLSSEMTMWPPICAAGENGDLYACCRRDLMMMKDDTWYKVGNL 301

Query: 301 AATTAKEGMVGVELGERAMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACY 341
            A       V +      +V G    G     +  +  N ++ ++E   ++ G VQ+ C+
Sbjct: 302 PADVCNVSYVAIRRSGNLVVIGSARYGEPSVGYNWDMSNSRWLKLETHDKYEGHVQAGCF 359

BLAST of MC05g1162 vs. ExPASy Swiss-Prot
Match: Q9M8L2 (F-box/kelch-repeat protein At1g80440 OS=Arabidopsis thaliana OX=3702 GN=At1g80440 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.7e-47
Identity = 102/245 (41.63%), Postives = 136/245 (55.51%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIP +P+++A ECL RS Y    V A VCR W +      F   R  S  + + + L Q
Sbjct: 2   ELIPNLPDDVARECLLRSSYQQFPVIASVCRAWNREVSLSQFLHQRKASRHSQELLILSQ 61

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
              A+  P G+    +   Y +SV +  +G WT + PI     GLPLFCR++S    L+V
Sbjct: 62  ---ARVDPAGSGKIIATPEYRISVLESGSGLWTELPPIPGQTKGLPLFCRLVSVGSDLIV 121

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHGGE-IFVAGGHDQGKN 180
           +GG DP T++  + VFV+ F T +WR G  MP  RRSFFG A+     + VAGGH++ K 
Sbjct: 122 LGGLDPITWQAHDSVFVFSFLTSKWRVGATMPGVRRSFFGCASDSDRTVLVAGGHNEEKC 181

Query: 181 AAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDT 240
           A  +A  YDV +D+W  LP M   RDEC+AV       V+ GY TE QG F  +AE FD 
Sbjct: 182 ALTSAMVYDVSEDKWTFLPDMARERDECKAVFHAGRFHVIGGYATEEQGQFSKTAESFDV 241

Query: 241 KTGTW 244
            T  W
Sbjct: 242 STWEW 243

BLAST of MC05g1162 vs. ExPASy Swiss-Prot
Match: Q9M1Y1 (F-box/kelch-repeat protein SKIP20 OS=Arabidopsis thaliana OX=3702 GN=SKIP20 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.4e-45
Identity = 106/294 (36.05%), Postives = 152/294 (51.70%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           +LIPG+PEE+A+ECL R  +  H     VCR W+ +     F K R   G+    + L+Q
Sbjct: 14  DLIPGLPEELAIECLVRVPFQFHSSIKSVCRSWKCVISSRSFIKERIGFGKAESLLCLVQ 73

Query: 61  SLSAQAGP---DGAKAA----------------------TSANAYGVSVFDPAAGNWTRI 120
            L++   P   +G + +                      T    YG+SV++     W R+
Sbjct: 74  PLTSPPSPAMMEGGEMSQKKKEEEEGESQMTQQLLQPRITGTPLYGLSVYNATLDTWHRV 133

Query: 121 EPIGKYPNGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEF-----ATERWRQG 180
                 P  +PLFC    I   GK+++IGGWDP T +PV DVFV +F     +  R+R+G
Sbjct: 134 ----AIPERIPLFCECVAIQDAGKVLLIGGWDPETLQPVRDVFVLDFFAGEGSGRRFRRG 193

Query: 181 KAMPERRSFFGAAAHGG-EIFVAGGHDQGKNAAATAWAYDVRQDEWRELPAMGAARDECE 240
           + M   RSFF  A+ G  +++VAGGHD  KNA  +A  YDV +DEW  LP M   RDEC 
Sbjct: 194 RPMSAARSFFACASVGSTKVYVAGGHDDQKNALRSAEVYDVEKDEWSMLPPMTEGRDECH 253

Query: 241 AVAIGSE--IWVVSGYGTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERCPRG 259
             ++ ++    V+SGYGTE QG F    E++D  T +W  ++  W   +  PRG
Sbjct: 254 GFSMATDPGFCVLSGYGTETQGQFRSDGEIYDPITNSWSTIENVWPFPDLSPRG 303

BLAST of MC05g1162 vs. ExPASy Swiss-Prot
Match: Q0WW40 (F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g16250 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.5e-16
Identity = 74/292 (25.34%), Postives = 127/292 (43.49%), Query Frame = 0

Query: 2   LIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQS 61
           +IPG+P+++AL C+ +  +  H V   V R WR L  G D+   + ++G +   + ++  
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVL-- 67

Query: 62  LSAQAGPDGAKAATSANAYGVSVFDPAAGNW-----TRIEPIGKYPNGLPLFCRIISSKG 121
                        T  +      +DP A  W     TR    G + +G    C    S  
Sbjct: 68  -------------TERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVC---VSNC 127

Query: 122 KLVVIGGWDPAT-----YRPV--EDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFV 181
            LV+ G + P+       +PV  +DV  ++   ++W+   +M   R+ F   +  G+++V
Sbjct: 128 LLVIGGCYAPSVSSFPHQKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYV 187

Query: 182 AGGHD-QGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVS---GYGTEN 241
           AGG +        +A  YD   D W ELPAM   + +C  ++      V+S   G+  +N
Sbjct: 188 AGGRNLTHSRGIPSAEVYDPVADRWEELPAMPRPQMDCSGLSYRGCFHVLSDQVGFAEQN 247

Query: 242 QGAFVDSAEVFDTKTGTWRRVDGAWKGERCPRGVVGVRGGGGIY---NWAEA 275
                 S+EVF+ +  TW  V+  W   R  +  V V     +Y   +W E+
Sbjct: 248 ------SSEVFNPRDMTWSTVEDVWPFSRAMQFAVQVMKNDRVYTIVDWGES 275

BLAST of MC05g1162 vs. NCBI nr
Match: XP_038904459.1 (F-box/kelch-repeat protein At2g44130-like [Benincasa hispida])

HSP 1 Score: 503 bits (1296), Expect = 7.18e-177
Identity = 254/341 (74.49%), Postives = 279/341 (81.82%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRWR LFL  DFY LR  SGRTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWRHLFLSRDFYNLRKLSGRTHKAVFAVQ 63

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL A    DGAK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCR+I   GKLVV
Sbjct: 64  SLPAPVS-DGAKSAVPV-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRLIGVDGKLVV 123

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDPA++RPVEDVFVYEFA E+WRQGK MPE+RSFFGAA +GGEIFVAGGHD+GKNAA
Sbjct: 124 IGGWDPASFRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGAAEYGGEIFVAGGHDEGKNAA 183

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           ATAWAY+VR DEWRELPAM   RDECEAVAIGSEIWVVSGYGTE QG F  +AEVFDTKT
Sbjct: 184 ATAWAYNVRNDEWRELPAMSGGRDECEAVAIGSEIWVVSGYGTEIQGNFETTAEVFDTKT 243

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAK-EGMVGVELGERAMVFG 300
           G WRRVD AW GER PRGVVGV   G +++WAE  AA  T    EG+VGV + ++AMVF 
Sbjct: 244 GEWRRVDSAWCGERSPRGVVGVGREGELFSWAEVMAATTTAVTAEGIVGVNMMDKAMVFV 303

Query: 301 WEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
                G+YGIF  EGQNGKFE++EVP EFSGFVQSACYTEI
Sbjct: 304 -----GRYGIFTAEGQNGKFEKIEVPEEFSGFVQSACYTEI 337

BLAST of MC05g1162 vs. NCBI nr
Match: XP_004136552.3 (F-box/kelch-repeat protein At2g44130 [Cucumis sativus])

HSP 1 Score: 475 bits (1222), Expect = 1.45e-165
Identity = 242/342 (70.76%), Postives = 271/342 (79.24%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 6   ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 65

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 66  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 125

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHD+GKNAA
Sbjct: 126 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 185

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 186 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 245

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAK--EGMVGVELGERAMVF 300
           G WRRV+ AW  ER PR VVGV   G ++NWA A AAA TTA   EG+VGV + E+A+VF
Sbjct: 246 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 305

Query: 301 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 306 M-----GRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 340

BLAST of MC05g1162 vs. NCBI nr
Match: KGN59267.1 (hypothetical protein Csa_002357 [Cucumis sativus])

HSP 1 Score: 475 bits (1222), Expect = 1.93e-165
Identity = 242/342 (70.76%), Postives = 271/342 (79.24%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 14  ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 73

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 74  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 133

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHD+GKNAA
Sbjct: 134 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 193

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 194 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 253

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAK--EGMVGVELGERAMVF 300
           G WRRV+ AW  ER PR VVGV   G ++NWA A AAA TTA   EG+VGV + E+A+VF
Sbjct: 254 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 313

Query: 301 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 314 M-----GRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 348

BLAST of MC05g1162 vs. NCBI nr
Match: XP_023528326.1 (F-box/kelch-repeat protein At2g44130-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 468 bits (1203), Expect = 9.78e-163
Identity = 233/340 (68.53%), Postives = 266/340 (78.24%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DFY LR  S RTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFYNLRRISDRTHKAVFAVQ 63

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL   AG DG K+ T+  A+G+S FDPA+G WT I+PIGKYPNGLPLFCR+I   GKLVV
Sbjct: 64  SLLLPAG-DGDKS-TAPPAFGLSAFDPASGKWTWIKPIGKYPNGLPLFCRLIGVDGKLVV 123

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDPA+YRPVEDVFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHD+ KNAA
Sbjct: 124 IGGWDPASYRPVEDVFVYDIAAEKWRQGKEMPATRSFFSAVECGGEIFVAGGHDEWKNAA 183

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           +TAWAY+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  SAE+F+T+T
Sbjct: 184 STAWAYNIKNDEWRELPAMSCGRDECEAVAIGSDIWVVSGYRTENQGNFEASAEIFNTET 243

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAKEGMVGVELGERAMVFGW 300
             WRRVD AW+ ER PRGVVGV   G +++WA A         EG+VGV +  +AMVF  
Sbjct: 244 EEWRRVDSAWREERSPRGVVGVSREGELFSWAAAVETTTAVTAEGVVGVNMEGKAMVFV- 303

Query: 301 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
               G++GIF REGQN KFERMEVP EF GFVQSACYTEI
Sbjct: 304 ----GRHGIFTREGQNSKFERMEVPEEFRGFVQSACYTEI 336

BLAST of MC05g1162 vs. NCBI nr
Match: XP_008443007.1 (PREDICTED: F-box/kelch-repeat protein At2g44130-like [Cucumis melo] >KAA0043760.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa] >TYK25373.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa])

HSP 1 Score: 468 bits (1203), Expect = 1.13e-162
Identity = 235/341 (68.91%), Postives = 264/341 (77.42%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKAV  +Q
Sbjct: 7   ELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKAVFAVQ 66

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL      +   AA  A  +GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 67  SLPLPVSDEAKSAAPIA--FGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 126

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHD+GKNAA
Sbjct: 127 IGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 186

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           ATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 187 ATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEVYETKT 246

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAKEGMVGVELGER-AMVFG 300
           G WRRV+ AW  ER PR VVGV   G ++NWA   A       EG+VGV + E+ AMVF 
Sbjct: 247 GKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKGAMVFM 306

Query: 301 WEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
                G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 307 -----GRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

BLAST of MC05g1162 vs. ExPASy TrEMBL
Match: A0A0A0LEY5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G792070 PE=4 SV=1)

HSP 1 Score: 475 bits (1222), Expect = 9.37e-166
Identity = 242/342 (70.76%), Postives = 271/342 (79.24%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 14  ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 73

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 74  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 133

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHD+GKNAA
Sbjct: 134 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 193

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 194 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 253

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAK--EGMVGVELGERAMVF 300
           G WRRV+ AW  ER PR VVGV   G ++NWA A AAA TTA   EG+VGV + E+A+VF
Sbjct: 254 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 313

Query: 301 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 314 M-----GRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 348

BLAST of MC05g1162 vs. ExPASy TrEMBL
Match: A0A5D3DP02 (F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005130 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 5.46e-163
Identity = 235/341 (68.91%), Postives = 264/341 (77.42%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKAV  +Q
Sbjct: 7   ELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKAVFAVQ 66

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL      +   AA  A  +GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 67  SLPLPVSDEAKSAAPIA--FGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 126

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHD+GKNAA
Sbjct: 127 IGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 186

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           ATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 187 ATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEVYETKT 246

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAKEGMVGVELGER-AMVFG 300
           G WRRV+ AW  ER PR VVGV   G ++NWA   A       EG+VGV + E+ AMVF 
Sbjct: 247 GKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKGAMVFM 306

Query: 301 WEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
                G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 307 -----GRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

BLAST of MC05g1162 vs. ExPASy TrEMBL
Match: A0A1S3B7S9 (F-box/kelch-repeat protein At2g44130-like OS=Cucumis melo OX=3656 GN=LOC103486736 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 5.46e-163
Identity = 235/341 (68.91%), Postives = 264/341 (77.42%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKAV  +Q
Sbjct: 7   ELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKAVFAVQ 66

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL      +   AA  A  +GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 67  SLPLPVSDEAKSAAPIA--FGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 126

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHD+GKNAA
Sbjct: 127 IGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 186

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           ATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 187 ATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEVYETKT 246

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAKEGMVGVELGER-AMVFG 300
           G WRRV+ AW  ER PR VVGV   G ++NWA   A       EG+VGV + E+ AMVF 
Sbjct: 247 GKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKGAMVFM 306

Query: 301 WEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
                G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 307 -----GRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

BLAST of MC05g1162 vs. ExPASy TrEMBL
Match: A0A6J1J0Y8 (F-box/kelch-repeat protein At2g44130-like OS=Cucurbita maxima OX=3661 GN=LOC111481691 PE=4 SV=1)

HSP 1 Score: 463 bits (1192), Expect = 2.23e-161
Identity = 231/340 (67.94%), Postives = 266/340 (78.24%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DF+ LR  S RTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFHNLRRISDRTHKAVFAVQ 63

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL   AG DG K+ T+  A+G+S FDPA G WT I+PI KY NGLPLFCR+I   GKLVV
Sbjct: 64  SLLVPAG-DGDKS-TAPLAFGLSAFDPATGKWTWIKPIEKYSNGLPLFCRLIGVDGKLVV 123

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDPA+YRPVEDVFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHD+ KNAA
Sbjct: 124 IGGWDPASYRPVEDVFVYDMAAEKWRQGKGMPATRSFFSAVECGGEIFVAGGHDEWKNAA 183

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           +TAWAY+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  +AE+FDT+T
Sbjct: 184 STAWAYNIKNDEWRELPAMSCGRDECEAVAIGSDIWVVSGYRTENQGNFEATAEIFDTET 243

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAKEGMVGVELGERAMVFGW 300
             WRRVD AW+ ER PRGVVGV   GG+++WA A AA      E +VGV +  +AMVF  
Sbjct: 244 EKWRRVDSAWREERSPRGVVGVSREGGLFSWAAAVAATTAVTAEEVVGVNMEGKAMVFV- 303

Query: 301 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
               G++GIF REGQN KFERMEVP +F+GFVQSACYTEI
Sbjct: 304 ----GRHGIFTREGQNSKFERMEVPEDFTGFVQSACYTEI 336

BLAST of MC05g1162 vs. ExPASy TrEMBL
Match: A0A6J1F4W9 (F-box/kelch-repeat protein At2g44130-like OS=Cucurbita moschata OX=3662 GN=LOC111442196 PE=4 SV=1)

HSP 1 Score: 454 bits (1169), Expect = 7.02e-158
Identity = 226/340 (66.47%), Postives = 261/340 (76.76%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DFY LR  S RTHK V  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFYNLRRISDRTHKVVFAVQ 63

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
           SL   AG DG K+ T+  A+G+S FDPA G WT I+PI KYPN LPLFCR+I   GKLVV
Sbjct: 64  SLLVPAG-DGDKS-TAPPAFGLSAFDPATGKWTWIKPIEKYPNRLPLFCRLIGVDGKLVV 123

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDQGKNAA 180
           IGGWDPA+YRPVE+VFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHD+ KNAA
Sbjct: 124 IGGWDPASYRPVEEVFVYDIAAEKWRQGKGMPATRSFFSAVEFGGEIFVAGGHDEWKNAA 183

Query: 181 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDTKT 240
           +TAW Y+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  +AE+F+T+T
Sbjct: 184 STAWVYNIKNDEWRELPAMSYGRDECEAVAIGSDIWVVSGYRTENQGNFEATAEIFNTET 243

Query: 241 GTWRRVDGAWKGERCPRGVVGVRGGGGIYNWAEADAAAATTAKEGMVGVELGERAMVFGW 300
             WRRVD AW+ ER PRGVVGV   G +++WA A         EG+VGV +  +AMVF  
Sbjct: 244 EEWRRVDSAWREERSPRGVVGVSREGELFSWAAAVETTTAVTAEGVVGVTMEGKAMVFV- 303

Query: 301 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 340
               G++GIF REG N KFERMEVP EF+GFVQSACYTEI
Sbjct: 304 ----GRHGIFTREGHNSKFERMEVPEEFTGFVQSACYTEI 336

BLAST of MC05g1162 vs. TAIR 10
Match: AT2G44130.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 196.4 bits (498), Expect = 3.6e-50
Identity = 109/283 (38.52%), Postives = 154/283 (54.42%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIPG+P E+ALECL R  +        VCR WR L     F + R + G+T   + L+Q
Sbjct: 18  ELIPGLPSELALECLVRVPFQFQSAMRSVCRSWRSLLSDSSFIQERRRCGKTELLLCLVQ 77

Query: 61  SLSAQAGP----------DGAKAATSAN-------AYGVSVFDPAAGNWTRIEPIGKYP- 120
            L+               D  K+   ++        +G+SV++ A   W R+     +P 
Sbjct: 78  PLTPPIPASKSVDETLMVDEKKSEDESHPRVFCTPRFGLSVYNAAMSTWHRV----AFPE 137

Query: 121 -NGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFG 180
              +PLFC   ++   GK+++IGGWDP T +P  DV+V EFA  +WR+G  M E RSFF 
Sbjct: 138 EEQIPLFCECVVLQDAGKILLIGGWDPETLQPTRDVYVLEFAGRKWRRGAPMKESRSFFA 197

Query: 181 -AAAHGGEIFVAGGHDQGKNAAATAWAYDVRQDEWRELPAMGAARDECE--AVAIGSEIW 240
            A+    +++VAGGHD  KNA  +A  YDV +DEW  +  M   RDEC+  AV +G    
Sbjct: 198 CASVSPTKVYVAGGHDDQKNALRSAEVYDVEKDEWSSVTPMTEGRDECQGFAVGMGLRFC 257

Query: 241 VVSGYGTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERCPRG 259
           V+SGYGTE+QG F    E++D  T +W R+D  W+  +  PRG
Sbjct: 258 VLSGYGTESQGRFRSDGEIYDPATDSWSRIDNVWRFPDTSPRG 296

BLAST of MC05g1162 vs. TAIR 10
Match: AT1G15670.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 195.3 bits (495), Expect = 8.1e-50
Identity = 123/363 (33.88%), Postives = 172/363 (47.38%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIP +PE +A ECL RS Y    + A VC+ W++     DF++ R  SG + + V L  
Sbjct: 2   ELIPDLPETVAYECLLRSSYKQFPLMASVCKLWQREISLSDFFRHRKASGHSQELVVL-- 61

Query: 61  SLSAQAGPDGAKAATSAN------AYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISS 120
              +QA  D  K   S N       Y +SV +   G  + + P+  + NGLPLFCR++S 
Sbjct: 62  ---SQARVDPVKELVSGNKTIPTPVYRISVLELGTGLRSELPPVPGHSNGLPLFCRLVSV 121

Query: 121 KGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHG-GEIFVAGG 180
              LVV+ G DP T+R  + VFV+ F T  WR GK+MP   RSFF  A+     +FVAGG
Sbjct: 122 GSDLVVLCGLDPVTWRTSDSVFVFSFLTSTWRVGKSMPGGPRSFFACASDSQRNVFVAGG 181

Query: 181 HDQGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDS 240
           HD+ KNA  +A  YDV +D W  LP MG  RDEC A+    +  V+ GY TE QG F  +
Sbjct: 182 HDEDKNAMMSALVYDVAEDRWAFLPDMGRERDECTAIFHAGKFHVIGGYSTEEQGQFSKT 241

Query: 241 AEVFDTKTGTWRRVDGAWKGERCPR--GVVGVRGGGGIY-------------NWAEADAA 300
           AE FD  T  W      +          +      G +Y              W +    
Sbjct: 242 AESFDVTTWRWSPQGEEFLSSEMTMWPPICAAGENGDLYACCRRDLMMMKDDTWYKVGNL 301

Query: 301 AATTAKEGMVGVELGERAMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACY 341
            A       V +      +V G    G     +  +  N ++ ++E   ++ G VQ+ C+
Sbjct: 302 PADVCNVSYVAIRRSGNLVVIGSARYGEPSVGYNWDMSNSRWLKLETHDKYEGHVQAGCF 359

BLAST of MC05g1162 vs. TAIR 10
Match: AT1G80440.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.6e-48
Identity = 102/245 (41.63%), Postives = 136/245 (55.51%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           ELIP +P+++A ECL RS Y    V A VCR W +      F   R  S  + + + L Q
Sbjct: 2   ELIPNLPDDVARECLLRSSYQQFPVIASVCRAWNREVSLSQFLHQRKASRHSQELLILSQ 61

Query: 61  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 120
              A+  P G+    +   Y +SV +  +G WT + PI     GLPLFCR++S    L+V
Sbjct: 62  ---ARVDPAGSGKIIATPEYRISVLESGSGLWTELPPIPGQTKGLPLFCRLVSVGSDLIV 121

Query: 121 IGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHGGE-IFVAGGHDQGKN 180
           +GG DP T++  + VFV+ F T +WR G  MP  RRSFFG A+     + VAGGH++ K 
Sbjct: 122 LGGLDPITWQAHDSVFVFSFLTSKWRVGATMPGVRRSFFGCASDSDRTVLVAGGHNEEKC 181

Query: 181 AAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYGTENQGAFVDSAEVFDT 240
           A  +A  YDV +D+W  LP M   RDEC+AV       V+ GY TE QG F  +AE FD 
Sbjct: 182 ALTSAMVYDVSEDKWTFLPDMARERDECKAVFHAGRFHVIGGYATEEQGQFSKTAESFDV 241

Query: 241 KTGTW 244
            T  W
Sbjct: 242 STWEW 243

BLAST of MC05g1162 vs. TAIR 10
Match: AT3G59940.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-46
Identity = 106/294 (36.05%), Postives = 152/294 (51.70%), Query Frame = 0

Query: 1   ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 60
           +LIPG+PEE+A+ECL R  +  H     VCR W+ +     F K R   G+    + L+Q
Sbjct: 14  DLIPGLPEELAIECLVRVPFQFHSSIKSVCRSWKCVISSRSFIKERIGFGKAESLLCLVQ 73

Query: 61  SLSAQAGP---DGAKAA----------------------TSANAYGVSVFDPAAGNWTRI 120
            L++   P   +G + +                      T    YG+SV++     W R+
Sbjct: 74  PLTSPPSPAMMEGGEMSQKKKEEEEGESQMTQQLLQPRITGTPLYGLSVYNATLDTWHRV 133

Query: 121 EPIGKYPNGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEF-----ATERWRQG 180
                 P  +PLFC    I   GK+++IGGWDP T +PV DVFV +F     +  R+R+G
Sbjct: 134 ----AIPERIPLFCECVAIQDAGKVLLIGGWDPETLQPVRDVFVLDFFAGEGSGRRFRRG 193

Query: 181 KAMPERRSFFGAAAHGG-EIFVAGGHDQGKNAAATAWAYDVRQDEWRELPAMGAARDECE 240
           + M   RSFF  A+ G  +++VAGGHD  KNA  +A  YDV +DEW  LP M   RDEC 
Sbjct: 194 RPMSAARSFFACASVGSTKVYVAGGHDDQKNALRSAEVYDVEKDEWSMLPPMTEGRDECH 253

Query: 241 AVAIGSE--IWVVSGYGTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERCPRG 259
             ++ ++    V+SGYGTE QG F    E++D  T +W  ++  W   +  PRG
Sbjct: 254 GFSMATDPGFCVLSGYGTETQGQFRSDGEIYDPITNSWSTIENVWPFPDLSPRG 303

BLAST of MC05g1162 vs. TAIR 10
Match: AT1G16250.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-17
Identity = 74/292 (25.34%), Postives = 127/292 (43.49%), Query Frame = 0

Query: 2   LIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQS 61
           +IPG+P+++AL C+ +  +  H V   V R WR L  G D+   + ++G +   + ++  
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVL-- 67

Query: 62  LSAQAGPDGAKAATSANAYGVSVFDPAAGNW-----TRIEPIGKYPNGLPLFCRIISSKG 121
                        T  +      +DP A  W     TR    G + +G    C    S  
Sbjct: 68  -------------TERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVC---VSNC 127

Query: 122 KLVVIGGWDPAT-----YRPV--EDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFV 181
            LV+ G + P+       +PV  +DV  ++   ++W+   +M   R+ F   +  G+++V
Sbjct: 128 LLVIGGCYAPSVSSFPHQKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYV 187

Query: 182 AGGHD-QGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVS---GYGTEN 241
           AGG +        +A  YD   D W ELPAM   + +C  ++      V+S   G+  +N
Sbjct: 188 AGGRNLTHSRGIPSAEVYDPVADRWEELPAMPRPQMDCSGLSYRGCFHVLSDQVGFAEQN 247

Query: 242 QGAFVDSAEVFDTKTGTWRRVDGAWKGERCPRGVVGVRGGGGIY---NWAEA 275
                 S+EVF+ +  TW  V+  W   R  +  V V     +Y   +W E+
Sbjct: 248 ------SSEVFNPRDMTWSTVEDVWPFSRAMQFAVQVMKNDRVYTIVDWGES 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O805825.1e-4938.52F-box/kelch-repeat protein At2g44130 OS=Arabidopsis thaliana OX=3702 GN=At2g4413... [more]
Q9LMR51.1e-4833.88F-box/kelch-repeat protein At1g15670 OS=Arabidopsis thaliana OX=3702 GN=At1g1567... [more]
Q9M8L23.7e-4741.63F-box/kelch-repeat protein At1g80440 OS=Arabidopsis thaliana OX=3702 GN=At1g8044... [more]
Q9M1Y13.4e-4536.05F-box/kelch-repeat protein SKIP20 OS=Arabidopsis thaliana OX=3702 GN=SKIP20 PE=1... [more]
Q0WW401.5e-1625.34F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g1625... [more]
Match NameE-valueIdentityDescription
XP_038904459.17.18e-17774.49F-box/kelch-repeat protein At2g44130-like [Benincasa hispida][more]
XP_004136552.31.45e-16570.76F-box/kelch-repeat protein At2g44130 [Cucumis sativus][more]
KGN59267.11.93e-16570.76hypothetical protein Csa_002357 [Cucumis sativus][more]
XP_023528326.19.78e-16368.53F-box/kelch-repeat protein At2g44130-like [Cucurbita pepo subsp. pepo][more]
XP_008443007.11.13e-16268.91PREDICTED: F-box/kelch-repeat protein At2g44130-like [Cucumis melo] >KAA0043760.... [more]
Match NameE-valueIdentityDescription
A0A0A0LEY59.37e-16670.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G792070 PE=4 SV=1[more]
A0A5D3DP025.46e-16368.91F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3B7S95.46e-16368.91F-box/kelch-repeat protein At2g44130-like OS=Cucumis melo OX=3656 GN=LOC10348673... [more]
A0A6J1J0Y82.23e-16167.94F-box/kelch-repeat protein At2g44130-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F4W97.02e-15866.47F-box/kelch-repeat protein At2g44130-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT2G44130.13.6e-5038.52Galactose oxidase/kelch repeat superfamily protein [more]
AT1G15670.18.1e-5033.88Galactose oxidase/kelch repeat superfamily protein [more]
AT1G80440.12.6e-4841.63Galactose oxidase/kelch repeat superfamily protein [more]
AT3G59940.12.4e-4636.05Galactose oxidase/kelch repeat superfamily protein [more]
AT1G16250.11.1e-1725.34Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 166..213
e-value: 8.7E-5
score: 31.9
coord: 214..264
e-value: 6.3
score: 4.7
coord: 117..165
e-value: 8.6E-9
score: 45.2
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 113..152
e-value: 7.3E-7
score: 28.7
coord: 154..199
e-value: 7.5E-12
score: 44.6
coord: 204..246
e-value: 1.5E-5
score: 24.4
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 51..301
e-value: 2.1E-41
score: 143.8
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 80..262
NoneNo IPR availablePANTHERPTHR46407:SF6F-BOX/KELCH-REPEAT PLANT-LIKE PROTEINcoord: 1..340
IPR044595F-box/kelch-repeat protein KMD1/2-likePANTHERPTHR46407OS02G0208700 PROTEINcoord: 1..340
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 3..46

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g1162.1MC05g1162.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0080037 negative regulation of cytokinin-activated signaling pathway
biological_process GO:2000762 regulation of phenylpropanoid metabolic process
molecular_function GO:0005515 protein binding