MC05g1130 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1130
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Delta(12)-fatty-acid desaturase
Location	MC05: 15442829 .. 15446641 (+)
RNA-Seq Expression	MC05g1130
Synteny	MC05g1130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCCAAGGCAGAGAGGCGATTGCGCAAAGCAAGATAAGGAACCAATAAGATAACGACACGTTAGTACCAGTTGTGCTGAGCCATGGAAATGGGCGGGCCCACAGCCTCACGTGCCGCTAGTGCTTAAATACCAGATGCTCATATTCCAAGAAGTGCCTCCAAAATAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTTCGTTGCCGGCGATCTGAAGGCGGCCATAGCAGAGAGAGATTGAGAGAGCGGAGAGTGCGAGGCTCTCTGAAACTTCGGTCCTCTTCTTCTCTTTCAGGTACTGTTCCGATCACATTCTGTACTTTTCCTTCTTTTGTTTGCTCTTTGATTCTCTAGTTTTTGTTTTGAGGCCAATGTGATGGGACTTGTTGGTTGCTTTTCATTTACGTTCGTGGTGATGCACAGATCTATGTCGAGATTAGATTACCGTTCACTGAGGTGTTTCTTGGCTTTTGCTTTCGACGTTTTTTTACCTCTGTATAATTTTGATTTTACAGAGATTATAGTTCGTATAATGCTTAGAGTTTAGGCTATAACGGAGCTTTTGAGCATTGTCGAAATGGTAGAGAGTTAGTCTGTAGGTTGTAGCTTGTTGTTGCTGAGATCGCGTGGTTTCGATCCGGATGCTTGGATTCGACTCTTTTCTGCAATGGCCACGCTATTGCGTCTGTAAATTTGTGATACCAAGGATTTGTCGATTTCTGAGCAAATCGCTAACTCGTATGCATTTTTCCCCTTTGTACGGATTTAGTCGTCGATTTGTTGTTGATCGGCGATATCTCGTACTCAACTTCCGAAAACATTTCCAAGAGAAGAAATTCTGGATGTCTTTCCCGGTTGGAAATCTTTGAAATCATAACTGAACTAATTTTTTTTACCAAATTATTTTTTGTCGTCTGTTTGTGATACTAATCCTTTATTTAAGGCAAATTTTTATAGATGAATTTTATTTATTTATTTTGTTATTTATGGCTCTAACTTTTAATGCTTTTTTCCTTTTGGCAAAGGTGCGTTTGGTGCTTTCCCTGTCTGTTCTCAAGTCAATTAGGGTAGGCGCCGTTTCGGTTTTAGCTTCCGTATCATTCAACGTGTGGTATTATGTGCTCAAAAATGAAAAGAAATTTCTTCTTTCCTTCAGTGTTTTGAGTTGAGCACCCGGCGATTATTAGAGGATAATTTATAGTCTTTCTGCAGAATGAATTTTAAAATTGTTTTTAACTCTGATTAGGTGAAATTAAATTGTTATAATAAATAAGCCATGTGTGCTATCAAATTTTAGGAGTTTTTTTTTTTTTTTCAAATAATGGACAATCAAACATATTTTTAATCAAATTTTATTTTTAAAAAAATCCCCATCAGTCCTCAAATCTACTTGAAAACTAATAAATCACAAAAGATCTTGGTCAATTGTGCAGCTTTGTGTTCTTTGTTCATTTTAGTTTGGGACATTCTTGCTTTCGTTTTAAATATGGAGAAAATAGGGTAGCCAGACAAGTCGAAATTATTAAAGACATTTTGGTTCTTAAAGAAACTGAAAACATAAACACAAAAGGAGAACGGAATTTACACGTTACGTCTCAGGTTTAGCTTTGAAACTCCAGTATTAAGGGATTCTATTGATACTGTAGATGGACAATATAAATTAGCTTACGGCATTAGAGAGTTGGAATTGGTCTTTAAACCTTATATTGATTTAATTGAGCTGTGTAATGAGCAGTTCTTTTTTCTCTCTTTTTTAAATGTTAGGTAGGATCAACTTGCGTCACTGTGAGAAGTGACAGCAATGTGATCGCTCTTTTAGGCTTTTTTCATGTACTTAGCTTGTCTAGTTGATAACCCGGACGGTTCCACTTGCGCATTAAATTATGTTAGAGAATCAGCGTAGACTTTGCCTACTAGTATTTTTATTTCCCTTTTTTCGCCTCAAGTGGGCGGCGAATGTCTAGCTTTGAAAGTTGGTTTTCTTTGTTTTGCCACTCCTAATTTCCTGAAAGGCGTGGTTGAACTATTATGTTGGTATTGAATTCACGTGTTCCCAAATTTTCCAATCCATACATGTGGGCCTGGTGTATTGACTCTTATTTGATGAATGAAGGTATCTTGAGGTCTAGAGTTTAATTACCCATTATATCTGAAAGTTTAATTTGTAGAGTTTAGGTAAATTATTGTAACATCTGTAGTCTACTAGATTTGACAGAAGGAGAAACTGTTTTCATAGTTTCAGAGATTTGAATAGGTCAATTATTTCCTTGGGTTTTTGTGAATGATTGTTTGTTTGCCTTTTGTGTACTTATGACTGGCTGTTTAATGATGCAATTTTTCCAGTCTCCTCAAACTCTTGCTTATTTTCAGGACAACATTCTTGAAAAATGGGTGCTGGAGGCCGAATGTCCTCTCCTCCATCCCTCAAGAAGTCAGATTCTGACCACTTGAAGCGAGTTCCATCCACAAAGCCCCCATTCACCCTTGGTGAGCTCAAAAAAGCCATTCCCCCACATTGCTTCCAACGCTCTGTTATCCGTTCATTCTCTTATGTCGTTTACGACATTACCATAGCTTCTATCTTCTATTACGTTGCAACCACTTACTTCCAAACTCTTCCTAGCCACTTCCAATACCCAGCCTGGGCTCTATACTGGGCCGCCCAAGGCTGCATCCTTACTGGCTTATGGGTCATTGCCCACGAGTGTGGCCACCACGCCTTCAGCGATTACCAGTGGCTCGATGACACCGTTGGCCTTATCCTCCACTCGTTCCTCATGGTCCCCTACTTCTCTTGGAAGTACAGCCACCGCAGACACCATTCCAACACCGGTTCTCTCGAGCGAGATGAAGTCTTTGTCCCTAAACAGAAATCCGCCATCAAATGGTACTCCAAATACCTCAACAACCCACCCGGCCGAGTCTTCACCCTCATCATCCAACTCGTCCTAGGCTGGCCTCTCTATTTGGCCTTCAACGTCTCCGGCCGACCCTACAACCGTTTCGCCTGCCACTACGACCCATACGGTCCCATCTACTCCGACCGAGAGCGCCTGCAGATATTCATCTCGGACGCCGGGTTGCTGGCCGTCTCGTACGGGCTCTACCGCCTCGTGGTGGCCAAGGGCCTGGCGTGGGTGGTGTGTGTGTATGGAGTGCCGCTCCTGGTGGTGAACGGGTTCCTGGTCCTAATCACATACTTGCAGCACACCCACCCGGCGCTGCCCCACTACGACTCCTCGGAGTGGGACTGGCTGAGAGGCGCGCTCGCAACAGTGGACAGAGACTACGGCATCTTGAACAAGGTGTTCCACAACATCACCGACACTCACGTGGCCCACCATTTGGTGTCCACCATGCCCCATTACCACGCCATGGAAGCAACGAAGGCGATAAAGCCAATCTTGGGGCAATACTACCACTTCGATGGCACCCCAATCTTCAAGGCCATGTATAGGGAGGCGAAGGAGTGCGTTTACGTGGAGCCTGACGAAGGTGAGAAGAATAAAGGCGTCTTCTGGTACAACAACAAGTTGGAATAAAACGTGTGGAAGCAGAGGGAAAGGTGCTTTCGGCTTTTGGCCTTTTGGAGGGCTCTTTAGACCTTTACCTATCCTGGTTTTAGTATTGTTTCCTTCAACTTAAAACCTTTGGATCTTAGTTTCTACTATGGGTTAAACTTTACTTACATAATCCCAACCTCGCTTTAGAACAGTTGAAGTTGTTTCTTTTTGATTGGTTTGCTTTGTAAACGTGGGTTCGGTTCTAGATTACGAGAGACTTATAAACTATTAAGTGCA

mRNA sequence

GGCCAAGGCAGAGAGGCGATTGCGCAAAGCAAGATAAGGAACCAATAAGATAACGACACGTTAGTACCAGTTGTGCTGAGCCATGGAAATGGGCGGGCCCACAGCCTCACGTGCCGCTAGTGCTTAAATACCAGATGCTCATATTCCAAGAAGTGCCTCCAAAATAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTTCGTTGCCGGCGATCTGAAGGCGGCCATAGCAGAGAGAGATTGAGAGAGCGGAGAGTGCGAGGCTCTCTGAAACTTCGGTCCTCTTCTTCTCTTTCAGGACAACATTCTTGAAAAATGGGTGCTGGAGGCCGAATGTCCTCTCCTCCATCCCTCAAGAAGTCAGATTCTGACCACTTGAAGCGAGTTCCATCCACAAAGCCCCCATTCACCCTTGGTGAGCTCAAAAAAGCCATTCCCCCACATTGCTTCCAACGCTCTGTTATCCGTTCATTCTCTTATGTCGTTTACGACATTACCATAGCTTCTATCTTCTATTACGTTGCAACCACTTACTTCCAAACTCTTCCTAGCCACTTCCAATACCCAGCCTGGGCTCTATACTGGGCCGCCCAAGGCTGCATCCTTACTGGCTTATGGGTCATTGCCCACGAGTGTGGCCACCACGCCTTCAGCGATTACCAGTGGCTCGATGACACCGTTGGCCTTATCCTCCACTCGTTCCTCATGGTCCCCTACTTCTCTTGGAAGTACAGCCACCGCAGACACCATTCCAACACCGGTTCTCTCGAGCGAGATGAAGTCTTTGTCCCTAAACAGAAATCCGCCATCAAATGGTACTCCAAATACCTCAACAACCCACCCGGCCGAGTCTTCACCCTCATCATCCAACTCGTCCTAGGCTGGCCTCTCTATTTGGCCTTCAACGTCTCCGGCCGACCCTACAACCGTTTCGCCTGCCACTACGACCCATACGGTCCCATCTACTCCGACCGAGAGCGCCTGCAGATATTCATCTCGGACGCCGGGTTGCTGGCCGTCTCGTACGGGCTCTACCGCCTCGTGGTGGCCAAGGGCCTGGCGTGGGTGGTGTGTGTGTATGGAGTGCCGCTCCTGGTGGTGAACGGGTTCCTGGTCCTAATCACATACTTGCAGCACACCCACCCGGCGCTGCCCCACTACGACTCCTCGGAGTGGGACTGGCTGAGAGGCGCGCTCGCAACAGTGGACAGAGACTACGGCATCTTGAACAAGGTGTTCCACAACATCACCGACACTCACGTGGCCCACCATTTGGTGTCCACCATGCCCCATTACCACGCCATGGAAGCAACGAAGGCGATAAAGCCAATCTTGGGGCAATACTACCACTTCGATGGCACCCCAATCTTCAAGGCCATGTATAGGGAGGCGAAGGAGTGCGTTTACGTGGAGCCTGACGAAGGTGAGAAGAATAAAGGCGTCTTCTGGTACAACAACAAGTTGGAATAAAACGTGTGGAAGCAGAGGGAAAGGTGCTTTCGGCTTTTGGCCTTTTGGAGGGCTCTTTAGACCTTTACCTATCCTGGTTTTAGTATTGTTTCCTTCAACTTAAAACCTTTGGATCTTAGTTTCTACTATGGGTTAAACTTTACTTACATAATCCCAACCTCGCTTTAGAACAGTTGAAGTTGTTTCTTTTTGATTGGTTTGCTTTGTAAACGTGGGTTCGGTTCTAGATTACGAGAGACTTATAAACTATTAAGTGCA

Coding sequence (CDS)

ATGGGTGCTGGAGGCCGAATGTCCTCTCCTCCATCCCTCAAGAAGTCAGATTCTGACCACTTGAAGCGAGTTCCATCCACAAAGCCCCCATTCACCCTTGGTGAGCTCAAAAAAGCCATTCCCCCACATTGCTTCCAACGCTCTGTTATCCGTTCATTCTCTTATGTCGTTTACGACATTACCATAGCTTCTATCTTCTATTACGTTGCAACCACTTACTTCCAAACTCTTCCTAGCCACTTCCAATACCCAGCCTGGGCTCTATACTGGGCCGCCCAAGGCTGCATCCTTACTGGCTTATGGGTCATTGCCCACGAGTGTGGCCACCACGCCTTCAGCGATTACCAGTGGCTCGATGACACCGTTGGCCTTATCCTCCACTCGTTCCTCATGGTCCCCTACTTCTCTTGGAAGTACAGCCACCGCAGACACCATTCCAACACCGGTTCTCTCGAGCGAGATGAAGTCTTTGTCCCTAAACAGAAATCCGCCATCAAATGGTACTCCAAATACCTCAACAACCCACCCGGCCGAGTCTTCACCCTCATCATCCAACTCGTCCTAGGCTGGCCTCTCTATTTGGCCTTCAACGTCTCCGGCCGACCCTACAACCGTTTCGCCTGCCACTACGACCCATACGGTCCCATCTACTCCGACCGAGAGCGCCTGCAGATATTCATCTCGGACGCCGGGTTGCTGGCCGTCTCGTACGGGCTCTACCGCCTCGTGGTGGCCAAGGGCCTGGCGTGGGTGGTGTGTGTGTATGGAGTGCCGCTCCTGGTGGTGAACGGGTTCCTGGTCCTAATCACATACTTGCAGCACACCCACCCGGCGCTGCCCCACTACGACTCCTCGGAGTGGGACTGGCTGAGAGGCGCGCTCGCAACAGTGGACAGAGACTACGGCATCTTGAACAAGGTGTTCCACAACATCACCGACACTCACGTGGCCCACCATTTGGTGTCCACCATGCCCCATTACCACGCCATGGAAGCAACGAAGGCGATAAAGCCAATCTTGGGGCAATACTACCACTTCGATGGCACCCCAATCTTCAAGGCCATGTATAGGGAGGCGAAGGAGTGCGTTTACGTGGAGCCTGACGAAGGTGAGAAGAATAAAGGCGTCTTCTGGTACAACAACAAGTTGGAATAA

Protein sequence

MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDITIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVFTLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLYRLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAKECVYVEPDEGEKNKGVFWYNNKLE

Homology

[Close]

BLAST of MC05g1130 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 3.1e-196
Identity = 309/382 (80.89%), Postives = 349/382 (91.36%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMS PP  KK +S+ LKRVP +KPPFTLG+LKKAIPPHCFQRSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSVPPPPKKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+A IFYY+AT YF  LP    Y AW +YWA QGC+LTG+WVIAHECGHHAFSDYQ LDD
Sbjct: 61  TVAFIFYYIATNYFHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
            VGL+LHS L+VPYFSWK+SHRRHHSNT SLERDEVFVPK+KS+I+W+SKYLNNPPGR+F
Sbjct: 121 IVGLVLHSCLLVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLF 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL I L LGWPLYLAFNVSGRPY+RFACHYDPYGPIY+DRER +I+ISDAG+LAV++GLY
Sbjct: 181 TLTITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RL  AKGLAWV+CVYGVPLL+VN FLV+ITYLQHTHP++PHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLAAAKGLAWVICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHL STMPHYHAMEATKAIKPILG+YY FDGTP +KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNK 383
           EC+YVE D+G+++KGV+WYN K
Sbjct: 361 ECIYVEADDGDESKGVYWYNKK 382

[Close]

BLAST of MC05g1130 vs. ExPASy Swiss-Prot
Match: P48631 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX=3847 GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 9.4e-193
Identity = 314/383 (81.98%), Postives = 340/383 (88.77%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGR   PP+ +KS+ D LKRVP  KP F+L ++KKAIPPHCFQRSV+RSFSYVVYD+
Sbjct: 1   MGAGGRTDVPPANRKSEVDPLKRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           TIA   YYVAT YF  LP    +   A+YWA QGCILTG+WVIAHECGHHAFSDYQ LDD
Sbjct: 61  TIAFCLYYVATHYFHLLPGPLSFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
            VGLILHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS IKWYSKYLNNPPGRV 
Sbjct: 121 IVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL + L LGWPLYLA NVSGRPY+RFACHYDPYGPIYSDRERLQI+ISDAG+LAV YGL+
Sbjct: 181 TLAVTLTLGWPLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLF 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RL +AKGLAWVVCVYGVPLLVVNGFLVLIT+LQHTHPALPHY SSEWDWLRGALATVDRD
Sbjct: 241 RLAMAKGLAWVVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHL STMPHYHAMEATKAIKPILG+YY FD TP  KAM+REA+
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREAR 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 384
           EC+YVEPD+  ++KGVFWYNNKL
Sbjct: 361 ECIYVEPDQSTESKGVFWYNNKL 383

[Close]

BLAST of MC05g1130 vs. ExPASy Swiss-Prot
Match: P46313 (Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 9.1e-188
Identity = 302/383 (78.85%), Postives = 334/383 (87.21%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRM  P S KKS++D  KRVP  KPPF++G+LKKAIPPHCF+RS+ RSFSY++ DI
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
            IAS FYYVAT YF  LP    Y AW LYWA QGC+LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWY KYLNNP GR+ 
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
            L +Q VLGWPLYLAFNVSGRPY+ FACH+ P  PIY+DRERLQI++SDAG+LAV +GLY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           R   A+G+A ++C+YGVPLL+VN FLVLITYLQHTHP+LPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHL STMPHY+AMEATKAIKPILG YY FDGTP + AMYREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 384
           EC+YVEPD     KGV+WYNNKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383

[Close]

BLAST of MC05g1130 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 652.5 bits (1682), Expect = 2.9e-186
Identity = 293/387 (75.71%), Postives = 336/387 (86.82%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLK----KSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYV 60
           MGAGGRM+ P   +    +     ++RVPS KPPFTL E+KKAIPPHCF+RS+++SFSYV
Sbjct: 1   MGAGGRMTVPNKWEGEGDEKSQKPVQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYV 60

Query: 61  VYDITIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQ 120
           +YD+T+ +IFYYVATTY   LP   +Y AW +YWA QGC+LTG+WVIAHECGHHAFSDYQ
Sbjct: 61  LYDLTLVAIFYYVATTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQ 120

Query: 121 WLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPP 180
           W+DD VGL+LHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPK KS + W+SKYLNNPP
Sbjct: 121 WVDDCVGLVLHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNPP 180

Query: 181 GRVFTLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVS 240
           GRV TLI+ L LGWPLYLA NVSG PY+RFACH+DPYGPIY+DRERLQI+ISD G++A +
Sbjct: 181 GRVMTLIVTLTLGWPLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAAT 240

Query: 241 YGLYRLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALAT 300
           Y LY++  A+GLAW+VCVYGVPLL+VN FLV ITYLQHTHPALPHYDSSEWDWLRGALAT
Sbjct: 241 YTLYKIAAARGLAWLVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALAT 300

Query: 301 VDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMY 360
            DRDYGILNKVFHNITDTHVAHHL STMPHYHAMEATKAIKPILG YY FDGTP++KAM+
Sbjct: 301 ADRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAMW 360

Query: 361 REAKECVYVEPDEGEKNKGVFWYNNKL 384
           REA+EC+YVEPD+G  +KGVFWY   L
Sbjct: 361 REARECLYVEPDDGANSKGVFWYKKNL 387

[Close]

BLAST of MC05g1130 vs. ExPASy Swiss-Prot
Match: Q39287 (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 PE=2 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 3.2e-185
Identity = 302/384 (78.65%), Postives = 330/384 (85.94%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRM   PS KKS++D LKRVP   PPFT+GELKKAIPPHCF+RS+ RSFSY+++DI
Sbjct: 1   MGAGGRMQVSPSPKKSETDTLKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
            +AS FYYVATTYF  LP    Y AW LYWA QG +LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  IVASCFYYVATTYFPLLPHPLSYVAWPLYWACQGVVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS IKWY KYLNNP GR  
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYGKYLNNPLGRTV 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPY-NRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGL 240
            L +Q  LGWPLY AFNVSGRPY   FACH+ P  PIY+DRERLQI++SDAG+LAV YGL
Sbjct: 181 MLTVQFTLGWPLYWAFNVSGRPYPEGFACHFHPNAPIYNDRERLQIYVSDAGILAVCYGL 240

Query: 241 YRLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDR 300
           YR   A+G+A +VC+YGVPLL+VN FLVLITYLQHTHP+LPHYDSSEWDWLRGALATVDR
Sbjct: 241 YRYAAAQGVASMVCLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300

Query: 301 DYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREA 360
           DYGILNKVFHNITDTHVAHHL STMPHYHAME TKAIKPILG YY FDGTP  KAM+REA
Sbjct: 301 DYGILNKVFHNITDTHVAHHLFSTMPHYHAMEVTKAIKPILGDYYQFDGTPWVKAMWREA 360

Query: 361 KECVYVEPDEGEKNKGVFWYNNKL 384
           KEC+YVEPD   + KGVFWYNNKL
Sbjct: 361 KECIYVEPDRQGEKKGVFWYNNKL 384

[Close]

BLAST of MC05g1130 vs. NCBI nr
Match: XP_022158335.1 (delta(12)-fatty-acid desaturase FAD2 [Momordica charantia])

HSP 1 Score: 810 bits (2093), Expect = 8.84e-297
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI
Sbjct: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY
Sbjct: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKLE 384
           ECVYVEPDEGEKNKGVFWYNNKLE
Sbjct: 361 ECVYVEPDEGEKNKGVFWYNNKLE 384

[Close]

BLAST of MC05g1130 vs. NCBI nr
Match: XP_008443062.2 (PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa] >TYK25404.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 754 bits (1947), Expect = 1.48e-274
Identity = 354/383 (92.43%), Postives = 370/383 (96.61%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP SLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYYVATTYFQ+LPS   YPAWALYWAAQGC+LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL IQLVLGWPLYLAFNVSGRPY+RFACHYDPYGPIYSDRERLQIFISDAGLLAV+YGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKG AWVVCVYGVPLL+VNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTP+ KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPDEGEK KG++WY NKL
Sbjct: 361 ECIYVEPDEGEK-KGIYWYKNKL 382

[Close]

BLAST of MC05g1130 vs. NCBI nr
Match: XP_022934859.1 (delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata])

HSP 1 Score: 754 bits (1947), Expect = 1.54e-274
Identity = 350/383 (91.38%), Postives = 368/383 (96.08%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP S+KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCF RSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYY+ATTYFQ LP+   YPAWALYWAAQGC+LTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL I LVLGWPLYLAFNVSGRPY+RFACHYDPYGPIY+DRERLQIFISDAGLLAVSYGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKGLAWVVCVYGVPLL+VNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP  KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPDEGEK KGVFWYNNK 
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNKF 383

[Close]

BLAST of MC05g1130 vs. NCBI nr
Match: KAG6580810.1 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 752 bits (1942), Expect = 8.91e-274
Identity = 349/383 (91.12%), Postives = 367/383 (95.82%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP S+KKSDSDHLKRVPS KPPFTLGELKKAIPPHCF RSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYY+ATTYFQ LP+   YPAWALYWAAQGC+LTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL I LVLGWPLYLAFNVSGRPY+RFACHYDPYGPIY+DRERLQIFISDAGLLAVSYGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKGLAWVVCVYGVPLL+VNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP  KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPDEGEK KGVFWYNNK 
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNKF 383

[Close]

BLAST of MC05g1130 vs. NCBI nr
Match: XP_038903906.1 (delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida])

HSP 1 Score: 752 bits (1941), Expect = 1.22e-273
Identity = 351/383 (91.64%), Postives = 369/383 (96.34%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP SLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYY+ATTYFQ LPS   YPAWA+YWAAQGC+LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWAVYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPLGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
            L IQLVLGWPLYLAFNVSGRPY+RFACHYDPYGPIYSDRERLQIFISDAGLLA++YGLY
Sbjct: 181 VLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAIAYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKG AWVVCVYGVPLL+VNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTP+ KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPDEG+K +GVFWY NKL
Sbjct: 361 ECIYVEPDEGDK-RGVFWYKNKL 382

[Close]

BLAST of MC05g1130 vs. ExPASy TrEMBL
Match: A0A6J1DVJ6 (delta(12)-fatty-acid desaturase FAD2 OS=Momordica charantia OX=3673 GN=LOC111024845 PE=3 SV=1)

HSP 1 Score: 810 bits (2093), Expect = 4.28e-297
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI
Sbjct: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY
Sbjct: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKLE 384
           ECVYVEPDEGEKNKGVFWYNNKLE
Sbjct: 361 ECVYVEPDEGEKNKGVFWYNNKLE 384

[Close]

BLAST of MC05g1130 vs. ExPASy TrEMBL
Match: A0A5A7TLK3 (Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005460 PE=3 SV=1)

HSP 1 Score: 754 bits (1947), Expect = 7.19e-275
Identity = 354/383 (92.43%), Postives = 370/383 (96.61%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP SLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYYVATTYFQ+LPS   YPAWALYWAAQGC+LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL IQLVLGWPLYLAFNVSGRPY+RFACHYDPYGPIYSDRERLQIFISDAGLLAV+YGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKG AWVVCVYGVPLL+VNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTP+ KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPDEGEK KG++WY NKL
Sbjct: 361 ECIYVEPDEGEK-KGIYWYKNKL 382

[Close]

BLAST of MC05g1130 vs. ExPASy TrEMBL
Match: A0A1S3B762 (delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=3 SV=1)

HSP 1 Score: 754 bits (1947), Expect = 7.19e-275
Identity = 354/383 (92.43%), Postives = 370/383 (96.61%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP SLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYYVATTYFQ+LPS   YPAWALYWAAQGC+LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL IQLVLGWPLYLAFNVSGRPY+RFACHYDPYGPIYSDRERLQIFISDAGLLAV+YGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKG AWVVCVYGVPLL+VNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTP+ KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPDEGEK KG++WY NKL
Sbjct: 361 ECIYVEPDEGEK-KGIYWYKNKL 382

[Close]

BLAST of MC05g1130 vs. ExPASy TrEMBL
Match: A0A6J1F3S1 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC111441899 PE=3 SV=1)

HSP 1 Score: 754 bits (1947), Expect = 7.46e-275
Identity = 350/383 (91.38%), Postives = 368/383 (96.08%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP S+KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCF RSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYY+ATTYFQ LP+   YPAWALYWAAQGC+LTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL I LVLGWPLYLAFNVSGRPY+RFACHYDPYGPIY+DRERLQIFISDAGLLAVSYGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKGLAWVVCVYGVPLL+VNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP  KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPDEGEK KGVFWYNNK 
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNKF 383

[Close]

BLAST of MC05g1130 vs. ExPASy TrEMBL
Match: A0A6J1J0W0 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC111481665 PE=3 SV=1)

HSP 1 Score: 751 bits (1939), Expect = 1.24e-273
Identity = 349/383 (91.12%), Postives = 366/383 (95.56%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRMSSP S+KKSDSDHLKRVPS KPPFTLGELKKAIPPHCF RSV+RSFSYVVYD+
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
           T+ASIFYY+ATTYFQ LPS   YPAWALYWAAQGC+LTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSA+KWYS YLNNP GRV 
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
           TL I LVLGWPLYLAFNVSGRPY+RFACHYDPYGPIY+DRERLQIFISDAGLLAVSYGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           RLV+AKGLAWVVCVYGVPLL+VNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP  KAM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 383
           EC+YVEPD GEK KGVFWYNNK 
Sbjct: 361 ECIYVEPDAGEKTKGVFWYNNKF 383

[Close]

BLAST of MC05g1130 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 657.5 bits (1695), Expect = 6.5e-189
Identity = 302/383 (78.85%), Postives = 334/383 (87.21%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRM  P S KKS++D  KRVP  KPPF++G+LKKAIPPHCF+RS+ RSFSY++ DI
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
            IAS FYYVAT YF  LP    Y AW LYWA QGC+LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWY KYLNNP GR+ 
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
            L +Q VLGWPLYLAFNVSGRPY+ FACH+ P  PIY+DRERLQI++SDAG+LAV +GLY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           R   A+G+A ++C+YGVPLL+VN FLVLITYLQHTHP+LPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHL STMPHY+AMEATKAIKPILG YY FDGTP + AMYREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 384
           EC+YVEPD     KGV+WYNNKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383

[Close]

BLAST of MC05g1130 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 657.5 bits (1695), Expect = 6.5e-189
Identity = 302/383 (78.85%), Postives = 334/383 (87.21%), Query Frame = 0


Query: 1   MGAGGRMSSPPSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDI 60
           MGAGGRM  P S KKS++D  KRVP  KPPF++G+LKKAIPPHCF+RS+ RSFSY++ DI
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  TIASIFYYVATTYFQTLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDD 120
            IAS FYYVAT YF  LP    Y AW LYWA QGC+LTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVF 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWY KYLNNP GR+ 
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 TLIIQLVLGWPLYLAFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLY 240
            L +Q VLGWPLYLAFNVSGRPY+ FACH+ P  PIY+DRERLQI++SDAG+LAV +GLY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RLVVAKGLAWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
           R   A+G+A ++C+YGVPLL+VN FLVLITYLQHTHP+LPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPIFKAMYREAK 360
           YGILNKVFHNITDTHVAHHL STMPHY+AMEATKAIKPILG YY FDGTP + AMYREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECVYVEPDEGEKNKGVFWYNNKL 384
           EC+YVEPD     KGV+WYNNKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383

[Close]

BLAST of MC05g1130 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 237.3 bits (604), Expect = 2.1e-62
Identity = 132/322 (40.99%), Postives = 186/322 (57.76%), Query Frame = 0


Query: 25  PSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDITIASIFYYVATTYFQTLPSHFQYP 84
           PS +PPF +G+++ AIP HC+ +S +RS SYVV DI IA     +A  Y  +      + 
Sbjct: 28  PSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDI-IAVAALAIAAVYVDS------WF 87

Query: 85  AWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRH 144
            W LYWAAQG +   ++V+ H+CGH +FSD   L+  VG ILHSF++VPY  W+ SHR H
Sbjct: 88  LWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTH 147

Query: 145 HSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVFTLIIQLVLGWPLYLAFNVSGRPYN 204
           H N G +E DE +VP  +   + Y K  ++     +T+ + + L +PLYL +   G+   
Sbjct: 148 HQNHGHVENDESWVPLPE---RVYKKLPHSTRMLRYTVPLPM-LAYPLYLCYRSPGKE-- 207

Query: 205 RFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLYRLVVAKGLAWVVCVYGVPLLVVNG 264
               H++PY  +++  ER  I  S      +   L  L    G   V+ VYGVP ++   
Sbjct: 208 --GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGVPYIIFVM 267

Query: 265 FLVLITYLQH--THPALPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLVS 324
           +L  +TYL H      LP Y   EW +LRG L T+DRDYGI N + H+I  THV HHL  
Sbjct: 268 WLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDI-GTHVIHHLFP 327

Query: 325 TMPHYHAMEATKAIKPILGQYY 345
            +PHYH ++ATKA K +LG+YY
Sbjct: 328 QIPHYHLVDATKAAKHVLGRYY 333

[Close]

BLAST of MC05g1130 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 219.9 bits (559), Expect = 3.5e-57
Identity = 131/356 (36.80%), Postives = 187/356 (52.53%), Query Frame = 0


Query: 25  PSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDITIASIFYYVATTYFQTLPSHFQYP 84
           P   PPF L +++ AIP HC+ ++  +S SYVV D+ I  +F   A   +        + 
Sbjct: 90  PGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAI--VFALAAGAAYLN-----NWI 149

Query: 85  AWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRH 144
            W LYW AQG +   L+V+ H+CGH +FS+   L+  VG +LHS ++VPY  W+ SHR H
Sbjct: 150 VWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTH 209

Query: 145 HSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVFTLIIQLV-LGWPLYLAFNVSGRPY 204
           H N G +E DE + P  +       K     P R F   + LV L +P YL     G+  
Sbjct: 210 HQNHGHVENDESWHPMSEKIYNTLDK-----PTRFFRFTLPLVMLAYPFYLWARSPGKK- 269

Query: 205 NRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLYRLVVAKGLAWVVCVYGVPLLVVN 264
                HY P   ++  +ER  +  S A   A++  L  L    G   ++ +YG+P  +  
Sbjct: 270 ---GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINV 329

Query: 265 GFLVLITYLQH--THPALPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLV 324
            +L  +TYL H      LP Y   EW +LRG L T+DRDYG++N + H+I  THV HHL 
Sbjct: 330 MWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVIHHLF 389

Query: 325 STMPHYHAMEATKAIKPILGQYYHFDGTP------IFKAMYREAKECVYVEPDEGE 372
             +PHYH +EAT+A KP+LG+YY            + + + +  KE  YV  DEGE
Sbjct: 390 PQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS-DEGE 427

[Close]

BLAST of MC05g1130 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 210.3 bits (534), Expect = 2.7e-54
Identity = 123/332 (37.05%), Postives = 177/332 (53.31%), Query Frame = 0


Query: 16  SDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVIRSFSYVVYDITIASIFYYVATTYFQ 75
           S+ D  +  P   PPF L +++ AIP HC+ ++   S SYVV D+ I      VA  YF 
Sbjct: 74  SEEDTERFDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVA-AYFN 133

Query: 76  TLPSHFQYPAWALYWAAQGCILTGLWVIAHECGHHAFSDYQWLDDTVGLILHSFLMVPYF 135
                  +  W LYW AQG +   L+V+ H+CGH +FS+   L+   G +LHS ++VPY 
Sbjct: 134 ------NWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYH 193

Query: 136 SWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYSKYLNNPPGRVFTLIIQL-VLGWPLYL 195
            W+ SHR HH N G +E DE + P  +S  K   K       ++F   +   +L +P YL
Sbjct: 194 GWRISHRTHHQNHGHVENDESWHPLPESIYKNLEK-----TTQMFRFTLPFPMLAYPFYL 253

Query: 196 AFNVSGRPYNRFACHYDPYGPIYSDRERLQIFISDAGLLAVSYGLYRLVVAKGLAWVVCV 255
                G+       HY P   ++  +E+  +  S A   A++  L  L    G   ++ +
Sbjct: 254 WNRSPGKQ----GSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKL 313

Query: 256 YGVPLLVVNGFLVLITYLQH--THPALPHYDSSEWDWLRGALATVDRDYGILNKVFHNIT 315
           YG+P  +   +L  +TYL H      LP Y   EW +LRG L T+DRDYG +N + H+I 
Sbjct: 314 YGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI- 373

Query: 316 DTHVAHHLVSTMPHYHAMEATKAIKPILGQYY 345
            THV HHL   +PHYH +EAT+A KP+LG+YY
Sbjct: 374 GTHVIHHLFPQIPHYHLVEATEAAKPVLGKYY 388

The following BLAST results are available for this feature:

BLAST of MC05g1130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GZC3	3.1e-196	80.89	Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV...	[more]
P48631	9.4e-193	81.98	Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX...	[more]
P46313	9.1e-188	78.85	Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=...	[more]
Q84VT2	2.9e-186	75.71	Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2	[more]
Q39287	3.2e-185	78.65	Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 ...	[more]

BLAST of MC05g1130 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158335.1	8.84e-297	100.00	delta(12)-fatty-acid desaturase FAD2 [Momordica charantia]	[more]
XP_008443062.2	1.48e-274	92.43	PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 del...	[more]
XP_022934859.1	1.54e-274	91.38	delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata]	[more]
KAG6580810.1	8.91e-274	91.12	Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbi...	[more]
XP_038903906.1	1.22e-273	91.64	delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida]	[more]

BLAST of MC05g1130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVJ6	4.28e-297	100.00	delta(12)-fatty-acid desaturase FAD2 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A5A7TLK3	7.19e-275	92.43	Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B762	7.19e-275	92.43	delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=...	[more]
A0A6J1F3S1	7.46e-275	91.38	delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J0W0	1.24e-273	91.12	delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of MC05g1130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12120.1	6.5e-189	78.85	fatty acid desaturase 2	[more]
AT3G12120.2	6.5e-189	78.85	fatty acid desaturase 2	[more]
AT2G29980.1	2.1e-62	40.99	fatty acid desaturase 3	[more]
AT3G11170.1	3.5e-57	36.80	fatty acid desaturase 7	[more]
AT5G05580.1	2.7e-54	37.05	fatty acid desaturase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 84..345 e-value: 4.3E-31 score: 108.7
IPR021863	Fatty acid desaturase, N-terminal	PFAM	PF11960	DUF3474	coord: 24..62 e-value: 2.2E-8 score: 34.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	PANTHER	PTHR32100	OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC	coord: 1..383
None	No IPR available	PANTHER	PTHR32100:SF40	OLEATE HYDROXYLASE FAH12	coord: 1..383
None	No IPR available	CDD	cd03507	Delta12-FADS-like	coord: 47..329 e-value: 1.55664E-81 score: 247.139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1130.1	MC05g1130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006636	unsaturated fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045485	omega-6 fatty acid desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water