MC05g1030 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1030 vs. ExPASy Swiss-Prot
Match: Q940A2 (Protein kinase and PP2C-like domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=At2g40860/At2g40870 PE=2 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 1.6e-256
Identity = 426/649 (65.64%), Postives = 525/649 (80.89%), Query Frame = 0

Query: 3   MGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKKPI 62
           M ++I  PNTC+RGCC+S+SIPLHL SS+FTLLSPIA+G+ES VYE  LDG++VA KKPI
Sbjct: 1   MVMEIVKPNTCIRGCCTSESIPLHLPSSSFTLLSPIAKGSESVVYEAILDGRRVAAKKPI 60

Query: 63  LSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEEWN 122
           LSTS+DLDKFH+ LQL C L+HP + KL+AA+AKPP+YMFFF+FYES  LAEKLH EEW+
Sbjct: 61  LSTSDDLDKFHRNLQLSCNLNHPGVAKLLAAHAKPPNYMFFFDFYESGTLAEKLHVEEWS 120

Query: 123 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHRKY 182
           PSI+QVL+ITL LAKALQYLHN GIVHRDVKPANV     LD+   PYLADFGLA ++K 
Sbjct: 121 PSIDQVLLITLHLAKALQYLHNNGIVHRDVKPANV----LLDEKFFPYLADFGLAEYKKN 180

Query: 183 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELLTG 242
           L+ ++ +NW+SSGKPTGGF+KK+MVGTLIYMAPEIL+K++++E +D+YSFGILINELLTG
Sbjct: 181 LREVNLQNWRSSGKPTGGFHKKNMVGTLIYMAPEILRKDMYTEKADIYSFGILINELLTG 240

Query: 243 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCWDA 302
           VVPYTD R EAQAHTVLEMNYTEQ LT AIVS GLRPAL  +    P   LSLI+ CW++
Sbjct: 241 VVPYTDRRAEAQAHTVLEMNYTEQQLTVAIVSSGLRPALAEIGLHLPKSLLSLIQNCWES 300

Query: 303 NPKNRPSFSDIVMDLESIWEDNKG------LESVTPVHPDLCGRLCDTNKNGYAYREDIN 362
           +P  RPS  ++V++LESIWE  +G      LE  +    D  G   D  KN   YR+ +N
Sbjct: 301 DPSKRPSSDNVVLELESIWEQVRGKQQGHLLEKTSNSQSDTDG--ADIIKNSGDYRDTVN 360

Query: 363 WSNQGEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPH 422
           W +QGE  S+++S +    V +W  S+     Y P++SCGSF+TCGRRE+MED+HF++PH
Sbjct: 361 WFSQGECLSKKSSVSTVFDVKLWSSSTDEPSRYVPVISCGSFATCGRRESMEDTHFIIPH 420

Query: 423 MCNEEDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKE 482
           MCNEE IH+F IFDGHRGAAAAEFSA+ +PG +++ CSTS+  +AL + FV+TD+ FR+E
Sbjct: 421 MCNEESIHLFAIFDGHRGAAAAEFSAQVLPGLVQSLCSTSA-GEALSQAFVRTDLAFRQE 480

Query: 483 LDFFRQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASC 542
           LD  RQSK+V QKDWHPGCTA+ +L+V ++LFVAN GD R ILCRAG+P  LSK H+A+C
Sbjct: 481 LDSHRQSKRVSQKDWHPGCTAIASLLVENKLFVANVGDSRAILCRAGHPFALSKAHLATC 540

Query: 543 LEERERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDY 602
           ++ER RVI  GG++ WLVDTWRV PA LQVTRSIGDDDLKPAVTAEPEI+ET+LS+ D++
Sbjct: 541 IDERNRVIGEGGRIEWLVDTWRVAPAGLQVTRSIGDDDLKPAVTAEPEISETILSADDEF 600

Query: 603 LVMASDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 646
           LVMASDGLWDV+++ EV+ IIRDTVKEP MCSKRLATEAAARGS DNIT
Sbjct: 601 LVMASDGLWDVMNDEEVIGIIRDTVKEPSMCSKRLATEAAARGSGDNIT 642

BLAST of MC05g1030 vs. ExPASy Swiss-Prot
Match: Q5JJY4 (Protein kinase and PP2C-like domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0541900 PE=2 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 7.6e-246
Identity = 419/650 (64.46%), Postives = 516/650 (79.38%), Query Frame = 0

Query: 3   MGLQI--ADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 62
           MG+++   + N CVRGCC S +IPLHL  S+F+LLSPIA+G+ESTVYE RL G++VA KK
Sbjct: 1   MGVEVPPEESNRCVRGCCRSAAIPLHLPPSSFSLLSPIAKGSESTVYEARLGGERVAAKK 60

Query: 63  PILSTSEDLDKFHKELQLL-----CELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEK 122
           P+LSTS+DLDKFH +LQLL      ELDHP + +LVAA+A+PP+Y+ FF+F+E PNLA+K
Sbjct: 61  PVLSTSDDLDKFHYQLQLLWWVLPIELDHPGLARLVAAHARPPNYLMFFDFFEPPNLADK 120

Query: 123 LHAEEWNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFG 182
           +H EEWNPS+ QV+ I  +LAKALQYL+ LGIVHRD+KPAN+     +DKD HP+LADFG
Sbjct: 121 IHVEEWNPSVQQVVTIATDLAKALQYLNILGIVHRDIKPANI----LIDKDFHPHLADFG 180

Query: 183 LAVHRKYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGIL 242
           LA+++K +K +S ENW+SSGKPTGGF+KK+MVGTLIYMAPEIL+K+IH+E SDVYSF I 
Sbjct: 181 LAMYQKDIKHVSVENWRSSGKPTGGFHKKNMVGTLIYMAPEILRKDIHTEKSDVYSFAIS 240

Query: 243 INELLTGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSL 302
           INELLTGVVPYTDLR EAQAHTVLEM YTEQ LTAAIVS GLRPAL   ESG P   LSL
Sbjct: 241 INELLTGVVPYTDLRAEAQAHTVLEMTYTEQQLTAAIVSQGLRPALALPESGAPPSLLSL 300

Query: 303 IRRCWDANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDI 362
           I+RCWD++P+ RPSF DI  +L+ I E +  + S +   P    +  + N   + Y+E +
Sbjct: 301 IQRCWDSDPQQRPSFKDITEELKII-EKHIAVNSCSLASP--ANKSQNGNTEVHHYQEAL 360

Query: 363 NWSNQGEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLP 422
           +W NQGE F++   +     V+ W D       Y P LS GSF+TCGRRETMED+HF+LP
Sbjct: 361 SWLNQGELFAK--GNKLDSTVDHWSDIFDQSSKYCPTLSWGSFATCGRRETMEDTHFMLP 420

Query: 423 HMCNEEDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRK 482
           HM  E+D+H FGIFDGHRG+AAAEFS RA+PGFL+   S +SP DAL E FV+TDI FR+
Sbjct: 421 HMSEEKDLHAFGIFDGHRGSAAAEFSVRAVPGFLKQFNSNTSPTDALTEAFVRTDIAFRE 480

Query: 483 ELDFFRQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVAS 542
           EL   ++SK++ QK+WHPGCTAV ALIVR++LFVANAGDCR IL RAG P  +++DHVAS
Sbjct: 481 ELILHQKSKRITQKNWHPGCTAVTALIVRNKLFVANAGDCRAILNRAGEPFPMTRDHVAS 540

Query: 543 CLEERERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDD 602
           C +ERER++  G +V W +DTWRVG AALQVTRSIGDDDLKPAVTA+PE+ ET+LS  D+
Sbjct: 541 CPKERERIVKEGTEVKWQIDTWRVGAAALQVTRSIGDDDLKPAVTAQPEVIETILSPDDE 600

Query: 603 YLVMASDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 646
           +LVMASDGLWDV+SN +V+SII+DTVKEPGMCSKRLATEAAARGS DNIT
Sbjct: 601 FLVMASDGLWDVMSNEDVLSIIKDTVKEPGMCSKRLATEAAARGSKDNIT 641

BLAST of MC05g1030 vs. ExPASy Swiss-Prot
Match: Q67UX7 (Probable protein phosphatase 2C 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0149800 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.4e-42
Identity = 112/290 (38.62%), Postives = 157/290 (54.14%), Query Frame = 0

Query: 357 WSNQGEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPH 416
           W    E    +   A  C+       S   L+ +   S G  S  G+R +MED +     
Sbjct: 50  WLEGTEELEEEMGFAGDCS-----PVSGGGLSENGKFSYGYASAPGKRASMEDFYETRID 109

Query: 417 MCNEEDIHVFGIFDGHRGAAAAEF-SARAIPGFLRNSCSTSSPADALVETFVKTDIEFRK 476
             + E I +FG+FDGH GA AAE+         +++    S    A+ ET+  TD EF K
Sbjct: 110 GVDGETIGLFGVFDGHGGARAAEYVKQHLFSNLIKHPKFISDIKSAIAETYNHTDSEFLK 169

Query: 477 ELDFFRQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVAS 536
                  ++    +D   G TA  A++V DRL VAN GD R ++CR G+ I +S+DH   
Sbjct: 170 -------AESSHTRD--AGSTASTAILVGDRLLVANVGDSRAVVCRGGDAIAVSRDHKPD 229

Query: 537 CLEERERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDD 596
             +ER+R+  AGG V W   TWRVG   L V+R+ GD  LK  V A+PEI E ++ S  +
Sbjct: 230 QSDERQRIEDAGGFVMW-AGTWRVG-GVLAVSRAFGDKLLKQYVVADPEIKEEIVDSSLE 289

Query: 597 YLVMASDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 646
           +L++ASDGLWDV+SN E V ++R  +++P   +KRL  EA  RGS DNIT
Sbjct: 290 FLILASDGLWDVVSNKEAVDMVR-PIQDPEQAAKRLLQEAYQRGSADNIT 322

BLAST of MC05g1030 vs. ExPASy Swiss-Prot
Match: Q8RXV3 (Probable protein phosphatase 2C 59 OS=Arabidopsis thaliana OX=3702 GN=WIN2 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 3.2e-42
Identity = 108/264 (40.91%), Postives = 151/264 (57.20%), Query Frame = 0

Query: 383 STADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEEDIHVFGIFDGHRGAAAAEF-S 442
           S   L+ +   S G  S+ G+R +MED +         E + +FG+FDGH GA AAE+  
Sbjct: 22  SGGGLSQNGKFSYGYASSPGKRSSMEDFYETRIDGVEGEIVGLFGVFDGHGGARAAEYVK 81

Query: 443 ARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQSKKVKQKDWHPGCTAVVAL 502
                  +R+    S    A+ + + +TD EF K       S+  + +D   G TA  A+
Sbjct: 82  QNLFSNLIRHPKFISDTTAAIADAYNQTDSEFLK-------SENSQNRD--AGSTASTAI 141

Query: 503 IVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERERVISAGGQVTWLVDTWRVGP 562
           +V DRL VAN GD R ++CR GN I +S+DH     +ER+R+  AGG V W   TWRVG 
Sbjct: 142 LVGDRLLVANVGDSRAVICRGGNAIAVSRDHKPDQSDERQRIEDAGGFVMW-AGTWRVG- 201

Query: 563 AALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASDGLWDVLSNSEVVSIIRDTV 622
             L V+R+ GD  LK  V A+PEI E  + S  ++L++ASDGLWDV+SN E V +I+  +
Sbjct: 202 GVLAVSRAFGDRLLKQYVVADPEIQEEKVDSSLEFLILASDGLWDVVSNEEAVGMIK-AI 261

Query: 623 KEPGMCSKRLATEAAARGSNDNIT 646
           ++P   +KRL  EA  RGS DNIT
Sbjct: 262 EDPEEGAKRLMMEAYQRGSADNIT 273

BLAST of MC05g1030 vs. ExPASy Swiss-Prot
Match: Q5Z6F5 (Probable protein phosphatase 2C 59 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0698300 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.5e-41
Identity = 102/253 (40.32%), Postives = 147/253 (58.10%), Query Frame = 0

Query: 394 SCGSFSTCGRRETMEDSHFLLPHMCNEEDIHVFGIFDGHRGAAAAEF-SARAIPGFLRNS 453
           S G  S+ G+R +MED +       + E + +FG+FDGH GA AAEF         +++ 
Sbjct: 64  SYGYASSPGKRSSMEDFYDTRIDGVDGETVGLFGVFDGHGGARAAEFVKQNLFTNLIKHP 123

Query: 454 CSTSSPADALVETFVKTDIEFRKELDFFRQSKKVKQKDWHPGCTAVVALIVRDRLFVANA 513
              S    A+ ET+  TD E  K       ++    +D   G TA  A++V DRL VAN 
Sbjct: 124 KLFSDTKSAIAETYTSTDSELLK-------AETSHNRD--AGSTASTAILVGDRLLVANV 183

Query: 514 GDCRTILCRAGNPIVLSKDHVASCLEERERVISAGGQVTWLVDTWRVGPAALQVTRSIGD 573
           GD R ++CR G+ I +S+DH     +ER+R+  AGG V W   TWRVG   L V+R+ GD
Sbjct: 184 GDSRAVICRGGDAIAVSRDHKPDQSDERQRIEDAGGFVMW-AGTWRVG-GVLAVSRAFGD 243

Query: 574 DDLKPAVTAEPEITETVLSSQDDYLVMASDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLA 633
             LK  V A+PEI E V+ S  ++L++ASDGLWDV++N E V++++  + +    +K+L 
Sbjct: 244 KLLKQYVVADPEIKEEVVDSSLEFLILASDGLWDVVTNEEAVAMVK-PILDSEQAAKKLL 303

Query: 634 TEAAARGSNDNIT 646
            EA+ RGS DNIT
Sbjct: 304 QEASQRGSADNIT 304

BLAST of MC05g1030 vs. NCBI nr
Match: XP_022147785.1 (protein kinase and PP2C-like domain-containing protein isoform X1 [Momordica charantia])

HSP 1 Score: 1291 bits (3342), Expect = 0.0
Identity = 640/645 (99.22%), Postives = 640/645 (99.22%), Query Frame = 0

Query: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60
           MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK
Sbjct: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60

Query: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120
           PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE
Sbjct: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120

Query: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHR 180
           WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV     LDKDLHPYLADFGLAVHR
Sbjct: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV----LLDKDLHPYLADFGLAVHR 180

Query: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240
           KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL
Sbjct: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240

Query: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300
           TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW
Sbjct: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300

Query: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360
           DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ
Sbjct: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360

Query: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420
           GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE
Sbjct: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420

Query: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480
           EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF
Sbjct: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480

Query: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540
           RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER
Sbjct: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540

Query: 541 ERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMA 600
           ERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMA
Sbjct: 541 ERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMA 600

Query: 601 SDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           SDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT
Sbjct: 601 SDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 641

BLAST of MC05g1030 vs. NCBI nr
Match: XP_038903907.1 (protein kinase and PP2C-like domain-containing protein [Benincasa hispida])

HSP 1 Score: 1142 bits (2955), Expect = 0.0
Identity = 563/645 (87.29%), Postives = 600/645 (93.02%), Query Frame = 0

Query: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60
           MDMGL+I  PNTC+RGCCSSKSIPLHLQ SAFTLLSPIARGAES VYEGRLDGKKVAVKK
Sbjct: 1   MDMGLEIVVPNTCIRGCCSSKSIPLHLQRSAFTLLSPIARGAESIVYEGRLDGKKVAVKK 60

Query: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120
           P LSTSE+LDKFHKELQLLCELDHP+IVKLVAANAKPP+YMFFFEFYESPNLAEKLHAEE
Sbjct: 61  PTLSTSEELDKFHKELQLLCELDHPAIVKLVAANAKPPNYMFFFEFYESPNLAEKLHAEE 120

Query: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHR 180
           WNPSINQVL +TL+LAKALQYLHNLGIVHRDVKPANV     LDKDLHPYLADFGLA+HR
Sbjct: 121 WNPSINQVLTVTLQLAKALQYLHNLGIVHRDVKPANV----LLDKDLHPYLADFGLAIHR 180

Query: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240
           KYLK ISAENW+SSGKPTGGFYKKSMVGTLIYMAPEIL+KEI+++LSDVYSFGIL+NELL
Sbjct: 181 KYLKEISAENWESSGKPTGGFYKKSMVGTLIYMAPEILRKEIYTDLSDVYSFGILMNELL 240

Query: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300
           TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVS GLRPALP  ESGTPSR LSLIRRCW
Sbjct: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSDGLRPALPSDESGTPSRLLSLIRRCW 300

Query: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360
           DANP NRPSFSDIVMDLESIWE+ KGLES TP+  DLCGRL D N++ Y YREDINWS+Q
Sbjct: 301 DANPINRPSFSDIVMDLESIWENRKGLESDTPIQSDLCGRLSDANEHDYVYREDINWSHQ 360

Query: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420
           GEH S+++SDA+RCA+N W D S   LAY P LS GSFSTCGRRETMED+HFLLPH C+E
Sbjct: 361 GEHCSQKSSDADRCALNEWLDFSADQLAYRPTLSWGSFSTCGRRETMEDTHFLLPHFCSE 420

Query: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480
           +DIHVFGIFDGHRGAAAAEFS +AIPGFLR S ST SP DAL+E FVKTDIEFRK+L+FF
Sbjct: 421 KDIHVFGIFDGHRGAAAAEFSDQAIPGFLRTSFSTRSPVDALMEAFVKTDIEFRKQLEFF 480

Query: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540
           R+SKKVKQKDWHPGCTAVVALIVR+RLFVANAGDCRTILCRAG+P+VLSKDHVASCLEER
Sbjct: 481 RKSKKVKQKDWHPGCTAVVALIVRERLFVANAGDCRTILCRAGDPVVLSKDHVASCLEER 540

Query: 541 ERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMA 600
           ERVISAGGQV W VDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVL+SQDDYLVMA
Sbjct: 541 ERVISAGGQVNWQVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLTSQDDYLVMA 600

Query: 601 SDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           SDGLWDVLSNSEVV IIRDTVK+PGMCSKRLATEAAARGSNDNIT
Sbjct: 601 SDGLWDVLSNSEVVGIIRDTVKDPGMCSKRLATEAAARGSNDNIT 641

BLAST of MC05g1030 vs. NCBI nr
Match: XP_022147786.1 (protein kinase and PP2C-like domain-containing protein isoform X2 [Momordica charantia])

HSP 1 Score: 1139 bits (2947), Expect = 0.0
Identity = 560/566 (98.94%), Postives = 561/566 (99.12%), Query Frame = 0

Query: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60
           MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK
Sbjct: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60

Query: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120
           PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE
Sbjct: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120

Query: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHR 180
           WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV     LDKDLHPYLADFGLAVHR
Sbjct: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV----LLDKDLHPYLADFGLAVHR 180

Query: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240
           KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL
Sbjct: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240

Query: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300
           TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW
Sbjct: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300

Query: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360
           DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ
Sbjct: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360

Query: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420
           GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE
Sbjct: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420

Query: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480
           EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF
Sbjct: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480

Query: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540
           RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER
Sbjct: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540

Query: 541 ERVISAGGQVTWLVDTWRVGPAALQV 566
           ERVISAGGQVTWLVDTWRVGPAALQ+
Sbjct: 541 ERVISAGGQVTWLVDTWRVGPAALQL 562

BLAST of MC05g1030 vs. NCBI nr
Match: XP_022971185.1 (protein kinase and PP2C-like domain-containing protein isoform X2 [Cucurbita maxima])

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 553/643 (86.00%), Postives = 594/643 (92.38%), Query Frame = 0

Query: 3   MGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKKPI 62
           MGLQI DPNTC+RGCCSSKSIPLHL  SAF+LLSPIARGAESTVYEGRLDGK+VA+KKPI
Sbjct: 1   MGLQIGDPNTCIRGCCSSKSIPLHLPPSAFSLLSPIARGAESTVYEGRLDGKRVAIKKPI 60

Query: 63  LSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEEWN 122
           LSTS+DLDKFH+ELQ+LCELDHP IVKL A NA+PP+Y+FFFEFYE PNLAEKLHAEEWN
Sbjct: 61  LSTSDDLDKFHRELQILCELDHPGIVKLFAVNARPPNYLFFFEFYEFPNLAEKLHAEEWN 120

Query: 123 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHRKY 182
           PSINQVL IT+ELAKALQYLHNLGIVHRDVKPANV     L KDL PYLADFGLAVH+K+
Sbjct: 121 PSINQVLTITIELAKALQYLHNLGIVHRDVKPANV----LLGKDLRPYLADFGLAVHQKH 180

Query: 183 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELLTG 242
           LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEIL+KEIH++LSDVYSFGIL+NELLTG
Sbjct: 181 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILRKEIHTDLSDVYSFGILMNELLTG 240

Query: 243 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCWDA 302
           VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVS GLRPALP  ESG PSR LSLIRRCWD 
Sbjct: 241 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSDGLRPALPNDESGAPSRLLSLIRRCWDE 300

Query: 303 NPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQGE 362
           NPKNRPSFSDIV+DLESIWED KGLESVTP+HP+LCG + D ++N YAY+EDINWSNQGE
Sbjct: 301 NPKNRPSFSDIVVDLESIWEDRKGLESVTPMHPNLCGSVSDASENDYAYQEDINWSNQGE 360

Query: 363 HFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEED 422
            FSR+AS+A+ CAVNVW DSS+   AY PILS GSFSTCGRRETMEDSHFLLP+MCN+ED
Sbjct: 361 QFSRKASNADECAVNVWLDSSSDHSAYRPILSWGSFSTCGRRETMEDSHFLLPNMCNKED 420

Query: 423 IHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQ 482
           IH FGIFDGHRGAAAAEFSA+AIPGFL+ SCST+SP DAL E FVKTD+EFRKEL+FFR+
Sbjct: 421 IHAFGIFDGHRGAAAAEFSAQAIPGFLQTSCSTTSPVDALKEAFVKTDVEFRKELNFFRK 480

Query: 483 SKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERER 542
           SKKVKQKDWHPGCT+ VALIVRDRLFVANAGDCRTILCRAG+P+VLSKDHVASC+EERER
Sbjct: 481 SKKVKQKDWHPGCTSAVALIVRDRLFVANAGDCRTILCRAGDPVVLSKDHVASCVEERER 540

Query: 543 VISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASD 602
           VI AGGQV W VDTWRVGPAALQVTRSIGDDDLKPAV+AEPEITETVLSSQDDYLVM SD
Sbjct: 541 VIKAGGQVKWQVDTWRVGPAALQVTRSIGDDDLKPAVSAEPEITETVLSSQDDYLVMGSD 600

Query: 603 GLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           GLWD LSNSEVV IIRDTVKE GMCSKRLATEA ARGS DNIT
Sbjct: 601 GLWDALSNSEVVGIIRDTVKEAGMCSKRLATEAVARGSKDNIT 639

BLAST of MC05g1030 vs. NCBI nr
Match: XP_022971187.1 (protein kinase and PP2C-like domain-containing protein isoform X3 [Cucurbita maxima])

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 553/643 (86.00%), Postives = 594/643 (92.38%), Query Frame = 0

Query: 3   MGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKKPI 62
           MGLQI DPNTC+RGCCSSKSIPLHL  SAF+LLSPIARGAESTVYEGRLDGK+VA+KKPI
Sbjct: 1   MGLQIGDPNTCIRGCCSSKSIPLHLPPSAFSLLSPIARGAESTVYEGRLDGKRVAIKKPI 60

Query: 63  LSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEEWN 122
           LSTS+DLDKFH+ELQ+LCELDHP IVKL A NA+PP+Y+FFFEFYE PNLAEKLHAEEWN
Sbjct: 61  LSTSDDLDKFHRELQILCELDHPGIVKLFAVNARPPNYLFFFEFYEFPNLAEKLHAEEWN 120

Query: 123 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHRKY 182
           PSINQVL IT+ELAKALQYLHNLGIVHRDVKPANV     L KDL PYLADFGLAVH+K+
Sbjct: 121 PSINQVLTITIELAKALQYLHNLGIVHRDVKPANV----LLGKDLRPYLADFGLAVHQKH 180

Query: 183 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELLTG 242
           LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEIL+KEIH++LSDVYSFGIL+NELLTG
Sbjct: 181 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILRKEIHTDLSDVYSFGILMNELLTG 240

Query: 243 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCWDA 302
           VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVS GLRPALP  ESG PSR LSLIRRCWD 
Sbjct: 241 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSDGLRPALPNDESGAPSRLLSLIRRCWDE 300

Query: 303 NPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQGE 362
           NPKNRPSFSDIV+DLESIWED KGLESVTP+HP+LCG + D ++N YAY+EDINWSNQGE
Sbjct: 301 NPKNRPSFSDIVVDLESIWEDRKGLESVTPMHPNLCGSVSDASENDYAYQEDINWSNQGE 360

Query: 363 HFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEED 422
            FSR+AS+A+ CAVNVW DSS+   AY PILS GSFSTCGRRETMEDSHFLLP+MCN+ED
Sbjct: 361 QFSRKASNADECAVNVWLDSSSDHSAYRPILSWGSFSTCGRRETMEDSHFLLPNMCNKED 420

Query: 423 IHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQ 482
           IH FGIFDGHRGAAAAEFSA+AIPGFL+ SCST+SP DAL E FVKTD+EFRKEL+FFR+
Sbjct: 421 IHAFGIFDGHRGAAAAEFSAQAIPGFLQTSCSTTSPVDALKEAFVKTDVEFRKELNFFRK 480

Query: 483 SKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERER 542
           SKKVKQKDWHPGCT+ VALIVRDRLFVANAGDCRTILCRAG+P+VLSKDHVASC+EERER
Sbjct: 481 SKKVKQKDWHPGCTSAVALIVRDRLFVANAGDCRTILCRAGDPVVLSKDHVASCVEERER 540

Query: 543 VISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASD 602
           VI AGGQV W VDTWRVGPAALQVTRSIGDDDLKPAV+AEPEITETVLSSQDDYLVM SD
Sbjct: 541 VIKAGGQVKWQVDTWRVGPAALQVTRSIGDDDLKPAVSAEPEITETVLSSQDDYLVMGSD 600

Query: 603 GLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           GLWD LSNSEVV IIRDTVKE GMCSKRLATEA ARGS DNIT
Sbjct: 601 GLWDALSNSEVVGIIRDTVKEAGMCSKRLATEAVARGSKDNIT 639

BLAST of MC05g1030 vs. ExPASy TrEMBL
Match: A0A6J1D140 (protein kinase and PP2C-like domain-containing protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016636 PE=4 SV=1)

HSP 1 Score: 1291 bits (3342), Expect = 0.0
Identity = 640/645 (99.22%), Postives = 640/645 (99.22%), Query Frame = 0

Query: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60
           MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK
Sbjct: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60

Query: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120
           PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE
Sbjct: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120

Query: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHR 180
           WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV     LDKDLHPYLADFGLAVHR
Sbjct: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV----LLDKDLHPYLADFGLAVHR 180

Query: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240
           KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL
Sbjct: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240

Query: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300
           TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW
Sbjct: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300

Query: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360
           DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ
Sbjct: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360

Query: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420
           GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE
Sbjct: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420

Query: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480
           EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF
Sbjct: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480

Query: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540
           RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER
Sbjct: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540

Query: 541 ERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMA 600
           ERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMA
Sbjct: 541 ERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMA 600

Query: 601 SDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           SDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT
Sbjct: 601 SDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 641

BLAST of MC05g1030 vs. ExPASy TrEMBL
Match: A0A6J1D3F0 (protein kinase and PP2C-like domain-containing protein isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016636 PE=4 SV=1)

HSP 1 Score: 1139 bits (2947), Expect = 0.0
Identity = 560/566 (98.94%), Postives = 561/566 (99.12%), Query Frame = 0

Query: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60
           MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK
Sbjct: 1   MDMGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKK 60

Query: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120
           PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE
Sbjct: 61  PILSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEE 120

Query: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHR 180
           WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV     LDKDLHPYLADFGLAVHR
Sbjct: 121 WNPSINQVLMITLELAKALQYLHNLGIVHRDVKPANV----LLDKDLHPYLADFGLAVHR 180

Query: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240
           KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL
Sbjct: 181 KYLKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELL 240

Query: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300
           TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW
Sbjct: 241 TGVVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCW 300

Query: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360
           DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ
Sbjct: 301 DANPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQ 360

Query: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420
           GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE
Sbjct: 361 GEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNE 420

Query: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480
           EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF
Sbjct: 421 EDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFF 480

Query: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540
           RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER
Sbjct: 481 RQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEER 540

Query: 541 ERVISAGGQVTWLVDTWRVGPAALQV 566
           ERVISAGGQVTWLVDTWRVGPAALQ+
Sbjct: 541 ERVISAGGQVTWLVDTWRVGPAALQL 562

BLAST of MC05g1030 vs. ExPASy TrEMBL
Match: A0A6J1I1A2 (protein kinase and PP2C-like domain-containing protein isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111469984 PE=4 SV=1)

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 553/643 (86.00%), Postives = 594/643 (92.38%), Query Frame = 0

Query: 3   MGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKKPI 62
           MGLQI DPNTC+RGCCSSKSIPLHL  SAF+LLSPIARGAESTVYEGRLDGK+VA+KKPI
Sbjct: 1   MGLQIGDPNTCIRGCCSSKSIPLHLPPSAFSLLSPIARGAESTVYEGRLDGKRVAIKKPI 60

Query: 63  LSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEEWN 122
           LSTS+DLDKFH+ELQ+LCELDHP IVKL A NA+PP+Y+FFFEFYE PNLAEKLHAEEWN
Sbjct: 61  LSTSDDLDKFHRELQILCELDHPGIVKLFAVNARPPNYLFFFEFYEFPNLAEKLHAEEWN 120

Query: 123 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHRKY 182
           PSINQVL IT+ELAKALQYLHNLGIVHRDVKPANV     L KDL PYLADFGLAVH+K+
Sbjct: 121 PSINQVLTITIELAKALQYLHNLGIVHRDVKPANV----LLGKDLRPYLADFGLAVHQKH 180

Query: 183 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELLTG 242
           LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEIL+KEIH++LSDVYSFGIL+NELLTG
Sbjct: 181 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILRKEIHTDLSDVYSFGILMNELLTG 240

Query: 243 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCWDA 302
           VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVS GLRPALP  ESG PSR LSLIRRCWD 
Sbjct: 241 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSDGLRPALPNDESGAPSRLLSLIRRCWDE 300

Query: 303 NPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQGE 362
           NPKNRPSFSDIV+DLESIWED KGLESVTP+HP+LCG + D ++N YAY+EDINWSNQGE
Sbjct: 301 NPKNRPSFSDIVVDLESIWEDRKGLESVTPMHPNLCGSVSDASENDYAYQEDINWSNQGE 360

Query: 363 HFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEED 422
            FSR+AS+A+ CAVNVW DSS+   AY PILS GSFSTCGRRETMEDSHFLLP+MCN+ED
Sbjct: 361 QFSRKASNADECAVNVWLDSSSDHSAYRPILSWGSFSTCGRRETMEDSHFLLPNMCNKED 420

Query: 423 IHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQ 482
           IH FGIFDGHRGAAAAEFSA+AIPGFL+ SCST+SP DAL E FVKTD+EFRKEL+FFR+
Sbjct: 421 IHAFGIFDGHRGAAAAEFSAQAIPGFLQTSCSTTSPVDALKEAFVKTDVEFRKELNFFRK 480

Query: 483 SKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERER 542
           SKKVKQKDWHPGCT+ VALIVRDRLFVANAGDCRTILCRAG+P+VLSKDHVASC+EERER
Sbjct: 481 SKKVKQKDWHPGCTSAVALIVRDRLFVANAGDCRTILCRAGDPVVLSKDHVASCVEERER 540

Query: 543 VISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASD 602
           VI AGGQV W VDTWRVGPAALQVTRSIGDDDLKPAV+AEPEITETVLSSQDDYLVM SD
Sbjct: 541 VIKAGGQVKWQVDTWRVGPAALQVTRSIGDDDLKPAVSAEPEITETVLSSQDDYLVMGSD 600

Query: 603 GLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           GLWD LSNSEVV IIRDTVKE GMCSKRLATEA ARGS DNIT
Sbjct: 601 GLWDALSNSEVVGIIRDTVKEAGMCSKRLATEAVARGSKDNIT 639

BLAST of MC05g1030 vs. ExPASy TrEMBL
Match: A0A6J1I519 (protein kinase and PP2C-like domain-containing protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469984 PE=4 SV=1)

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 553/643 (86.00%), Postives = 594/643 (92.38%), Query Frame = 0

Query: 3   MGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKKPI 62
           MGLQI DPNTC+RGCCSSKSIPLHL  SAF+LLSPIARGAESTVYEGRLDGK+VA+KKPI
Sbjct: 1   MGLQIGDPNTCIRGCCSSKSIPLHLPPSAFSLLSPIARGAESTVYEGRLDGKRVAIKKPI 60

Query: 63  LSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEEWN 122
           LSTS+DLDKFH+ELQ+LCELDHP IVKL A NA+PP+Y+FFFEFYE PNLAEKLHAEEWN
Sbjct: 61  LSTSDDLDKFHRELQILCELDHPGIVKLFAVNARPPNYLFFFEFYEFPNLAEKLHAEEWN 120

Query: 123 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHRKY 182
           PSINQVL IT+ELAKALQYLHNLGIVHRDVKPANV     L KDL PYLADFGLAVH+K+
Sbjct: 121 PSINQVLTITIELAKALQYLHNLGIVHRDVKPANV----LLGKDLRPYLADFGLAVHQKH 180

Query: 183 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELLTG 242
           LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEIL+KEIH++LSDVYSFGIL+NELLTG
Sbjct: 181 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILRKEIHTDLSDVYSFGILMNELLTG 240

Query: 243 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCWDA 302
           VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVS GLRPALP  ESG PSR LSLIRRCWD 
Sbjct: 241 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSDGLRPALPNDESGAPSRLLSLIRRCWDE 300

Query: 303 NPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLCDTNKNGYAYREDINWSNQGE 362
           NPKNRPSFSDIV+DLESIWED KGLESVTP+HP+LCG + D ++N YAY+EDINWSNQGE
Sbjct: 301 NPKNRPSFSDIVVDLESIWEDRKGLESVTPMHPNLCGSVSDASENDYAYQEDINWSNQGE 360

Query: 363 HFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEED 422
            FSR+AS+A+ CAVNVW DSS+   AY PILS GSFSTCGRRETMEDSHFLLP+MCN+ED
Sbjct: 361 QFSRKASNADECAVNVWLDSSSDHSAYRPILSWGSFSTCGRRETMEDSHFLLPNMCNKED 420

Query: 423 IHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQ 482
           IH FGIFDGHRGAAAAEFSA+AIPGFL+ SCST+SP DAL E FVKTD+EFRKEL+FFR+
Sbjct: 421 IHAFGIFDGHRGAAAAEFSAQAIPGFLQTSCSTTSPVDALKEAFVKTDVEFRKELNFFRK 480

Query: 483 SKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERER 542
           SKKVKQKDWHPGCT+ VALIVRDRLFVANAGDCRTILCRAG+P+VLSKDHVASC+EERER
Sbjct: 481 SKKVKQKDWHPGCTSAVALIVRDRLFVANAGDCRTILCRAGDPVVLSKDHVASCVEERER 540

Query: 543 VISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASD 602
           VI AGGQV W VDTWRVGPAALQVTRSIGDDDLKPAV+AEPEITETVLSSQDDYLVM SD
Sbjct: 541 VIKAGGQVKWQVDTWRVGPAALQVTRSIGDDDLKPAVSAEPEITETVLSSQDDYLVMGSD 600

Query: 603 GLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           GLWD LSNSEVV IIRDTVKE GMCSKRLATEA ARGS DNIT
Sbjct: 601 GLWDALSNSEVVGIIRDTVKEAGMCSKRLATEAVARGSKDNIT 639

BLAST of MC05g1030 vs. ExPASy TrEMBL
Match: A0A6J1F4X8 (protein kinase and PP2C-like domain-containing protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440856 PE=4 SV=1)

HSP 1 Score: 1127 bits (2916), Expect = 0.0
Identity = 554/644 (86.02%), Postives = 595/644 (92.39%), Query Frame = 0

Query: 3   MGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKKPI 62
           MGLQI DPNTC+RGCCSSKSIPLHL  SAF+LLSPIARGAESTVYEGRLDGK+VAVKKPI
Sbjct: 1   MGLQIGDPNTCIRGCCSSKSIPLHLPPSAFSLLSPIARGAESTVYEGRLDGKRVAVKKPI 60

Query: 63  LSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEEWN 122
           LSTS+DLDKFH+ELQ+LCELDHP IVKL+A NA+PP+Y+FFFEFYE PNL+EK+HAEEWN
Sbjct: 61  LSTSDDLDKFHRELQILCELDHPGIVKLIAVNARPPNYLFFFEFYEFPNLSEKVHAEEWN 120

Query: 123 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHRKY 182
           PSINQVLMITLELAKALQYLHNLGIVHRDVKPANV     L KDL PYLADFGLAVH+K+
Sbjct: 121 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANV----LLGKDLRPYLADFGLAVHQKH 180

Query: 183 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELLTG 242
           LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEIL+KEIH++LSDVYSFGIL+NELLTG
Sbjct: 181 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILRKEIHTDLSDVYSFGILMNELLTG 240

Query: 243 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCWDA 302
           VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVS GLRPALP  ESG PSR LSLIRRCWD 
Sbjct: 241 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSDGLRPALPNDESGAPSRLLSLIRRCWDE 300

Query: 303 NPKNRPSFSDIVMDLESIWEDNKGLESVTPVHPDLCGRLC-DTNKNGYAYREDINWSNQG 362
           NPKNRPSFSDIV+DLESIWED KGLESVTP+HP+LCG +  D ++N YAY+EDINWSNQG
Sbjct: 301 NPKNRPSFSDIVVDLESIWEDRKGLESVTPMHPNLCGSVVSDASENDYAYQEDINWSNQG 360

Query: 363 EHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEE 422
           E FSR+AS+A+ CAVNVW DSS+  LAY PILS GSFSTCGRRETMEDSHFLLP+MCN+E
Sbjct: 361 EQFSRKASNADGCAVNVWLDSSSDHLAYRPILSWGSFSTCGRRETMEDSHFLLPNMCNKE 420

Query: 423 DIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFR 482
           DIH FGIFDGHRGAAAAEFSA+AIPGFL+ SCST SP DAL E FVKTD+EFRKELDFFR
Sbjct: 421 DIHAFGIFDGHRGAAAAEFSAQAIPGFLQASCSTRSPVDALKEAFVKTDVEFRKELDFFR 480

Query: 483 QSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERE 542
           +SKKVKQKDWHPGCT+ VALIVRDRLFVANAGDCRTILCRAG+P+VLSKDHVASC+EERE
Sbjct: 481 KSKKVKQKDWHPGCTSAVALIVRDRLFVANAGDCRTILCRAGDPVVLSKDHVASCVEERE 540

Query: 543 RVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMAS 602
           RVI AGGQV W VDTWRVGPAALQVTRSIGDDDLKPAV+AEPEITETVL+SQDDYLVM S
Sbjct: 541 RVIRAGGQVRWQVDTWRVGPAALQVTRSIGDDDLKPAVSAEPEITETVLTSQDDYLVMGS 600

Query: 603 DGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 645
           DGLWD LSNSEVV IIRDTVKE  MCSKRLATEA ARGS DNIT
Sbjct: 601 DGLWDALSNSEVVGIIRDTVKEAAMCSKRLATEAVARGSKDNIT 640

BLAST of MC05g1030 vs. TAIR 10
Match: AT2G40860.1 (protein kinase family protein / protein phosphatase 2C ( PP2C) family protein )

HSP 1 Score: 886.7 bits (2290), Expect = 1.2e-257
Identity = 426/649 (65.64%), Postives = 525/649 (80.89%), Query Frame = 0

Query: 3   MGLQIADPNTCVRGCCSSKSIPLHLQSSAFTLLSPIARGAESTVYEGRLDGKKVAVKKPI 62
           M ++I  PNTC+RGCC+S+SIPLHL SS+FTLLSPIA+G+ES VYE  LDG++VA KKPI
Sbjct: 1   MVMEIVKPNTCIRGCCTSESIPLHLPSSSFTLLSPIAKGSESVVYEAILDGRRVAAKKPI 60

Query: 63  LSTSEDLDKFHKELQLLCELDHPSIVKLVAANAKPPSYMFFFEFYESPNLAEKLHAEEWN 122
           LSTS+DLDKFH+ LQL C L+HP + KL+AA+AKPP+YMFFF+FYES  LAEKLH EEW+
Sbjct: 61  LSTSDDLDKFHRNLQLSCNLNHPGVAKLLAAHAKPPNYMFFFDFYESGTLAEKLHVEEWS 120

Query: 123 PSINQVLMITLELAKALQYLHNLGIVHRDVKPANVGFLMQLDKDLHPYLADFGLAVHRKY 182
           PSI+QVL+ITL LAKALQYLHN GIVHRDVKPANV     LD+   PYLADFGLA ++K 
Sbjct: 121 PSIDQVLLITLHLAKALQYLHNNGIVHRDVKPANV----LLDEKFFPYLADFGLAEYKKN 180

Query: 183 LKGISAENWKSSGKPTGGFYKKSMVGTLIYMAPEILKKEIHSELSDVYSFGILINELLTG 242
           L+ ++ +NW+SSGKPTGGF+KK+MVGTLIYMAPEIL+K++++E +D+YSFGILINELLTG
Sbjct: 181 LREVNLQNWRSSGKPTGGFHKKNMVGTLIYMAPEILRKDMYTEKADIYSFGILINELLTG 240

Query: 243 VVPYTDLRTEAQAHTVLEMNYTEQLLTAAIVSGGLRPALPGLESGTPSRFLSLIRRCWDA 302
           VVPYTD R EAQAHTVLEMNYTEQ LT AIVS GLRPAL  +    P   LSLI+ CW++
Sbjct: 241 VVPYTDRRAEAQAHTVLEMNYTEQQLTVAIVSSGLRPALAEIGLHLPKSLLSLIQNCWES 300

Query: 303 NPKNRPSFSDIVMDLESIWEDNKG------LESVTPVHPDLCGRLCDTNKNGYAYREDIN 362
           +P  RPS  ++V++LESIWE  +G      LE  +    D  G   D  KN   YR+ +N
Sbjct: 301 DPSKRPSSDNVVLELESIWEQVRGKQQGHLLEKTSNSQSDTDG--ADIIKNSGDYRDTVN 360

Query: 363 WSNQGEHFSRQASDANRCAVNVWPDSSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPH 422
           W +QGE  S+++S +    V +W  S+     Y P++SCGSF+TCGRRE+MED+HF++PH
Sbjct: 361 WFSQGECLSKKSSVSTVFDVKLWSSSTDEPSRYVPVISCGSFATCGRRESMEDTHFIIPH 420

Query: 423 MCNEEDIHVFGIFDGHRGAAAAEFSARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKE 482
           MCNEE IH+F IFDGHRGAAAAEFSA+ +PG +++ CSTS+  +AL + FV+TD+ FR+E
Sbjct: 421 MCNEESIHLFAIFDGHRGAAAAEFSAQVLPGLVQSLCSTSA-GEALSQAFVRTDLAFRQE 480

Query: 483 LDFFRQSKKVKQKDWHPGCTAVVALIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASC 542
           LD  RQSK+V QKDWHPGCTA+ +L+V ++LFVAN GD R ILCRAG+P  LSK H+A+C
Sbjct: 481 LDSHRQSKRVSQKDWHPGCTAIASLLVENKLFVANVGDSRAILCRAGHPFALSKAHLATC 540

Query: 543 LEERERVISAGGQVTWLVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDY 602
           ++ER RVI  GG++ WLVDTWRV PA LQVTRSIGDDDLKPAVTAEPEI+ET+LS+ D++
Sbjct: 541 IDERNRVIGEGGRIEWLVDTWRVAPAGLQVTRSIGDDDLKPAVTAEPEISETILSADDEF 600

Query: 603 LVMASDGLWDVLSNSEVVSIIRDTVKEPGMCSKRLATEAAARGSNDNIT 646
           LVMASDGLWDV+++ EV+ IIRDTVKEP MCSKRLATEAAARGS DNIT
Sbjct: 601 LVMASDGLWDVMNDEEVIGIIRDTVKEPSMCSKRLATEAAARGSGDNIT 642

BLAST of MC05g1030 vs. TAIR 10
Match: AT4G31750.1 (HOPW1-1-interacting 2 )

HSP 1 Score: 174.9 bits (442), Expect = 2.3e-43
Identity = 108/264 (40.91%), Postives = 151/264 (57.20%), Query Frame = 0

Query: 383 STADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEEDIHVFGIFDGHRGAAAAEF-S 442
           S   L+ +   S G  S+ G+R +MED +         E + +FG+FDGH GA AAE+  
Sbjct: 22  SGGGLSQNGKFSYGYASSPGKRSSMEDFYETRIDGVEGEIVGLFGVFDGHGGARAAEYVK 81

Query: 443 ARAIPGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQSKKVKQKDWHPGCTAVVAL 502
                  +R+    S    A+ + + +TD EF K       S+  + +D   G TA  A+
Sbjct: 82  QNLFSNLIRHPKFISDTTAAIADAYNQTDSEFLK-------SENSQNRD--AGSTASTAI 141

Query: 503 IVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERERVISAGGQVTWLVDTWRVGP 562
           +V DRL VAN GD R ++CR GN I +S+DH     +ER+R+  AGG V W   TWRVG 
Sbjct: 142 LVGDRLLVANVGDSRAVICRGGNAIAVSRDHKPDQSDERQRIEDAGGFVMW-AGTWRVG- 201

Query: 563 AALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASDGLWDVLSNSEVVSIIRDTV 622
             L V+R+ GD  LK  V A+PEI E  + S  ++L++ASDGLWDV+SN E V +I+  +
Sbjct: 202 GVLAVSRAFGDRLLKQYVVADPEIQEEKVDSSLEFLILASDGLWDVVSNEEAVGMIK-AI 261

Query: 623 KEPGMCSKRLATEAAARGSNDNIT 646
           ++P   +KRL  EA  RGS DNIT
Sbjct: 262 EDPEEGAKRLMMEAYQRGSADNIT 273

BLAST of MC05g1030 vs. TAIR 10
Match: AT5G24940.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 171.4 bits (433), Expect = 2.5e-42
Identity = 104/265 (39.25%), Postives = 150/265 (56.60%), Query Frame = 0

Query: 382 SSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEEDIHVFGIFDGHRGAAAAEFS 441
           +S   L+ +   S G  S+ G+R +MED         + E + +FG+FDGH G+ AAE+ 
Sbjct: 21  ASGGGLSQNGKFSYGYASSAGKRSSMEDFFETRIDGIDGEIVGLFGVFDGHGGSRAAEYV 80

Query: 442 ARAI-PGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQSKKVKQKDWHPGCTAVVA 501
            R +    + +    S    A+ + +  TD E  K       S+    +D   G TA  A
Sbjct: 81  KRHLFSNLITHPKFISDTKSAIADAYTHTDSELLK-------SENSHTRD--AGSTASTA 140

Query: 502 LIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERERVISAGGQVTWLVDTWRVG 561
           ++V DRL VAN GD R ++CR GN   +S+DH     +ERER+ +AGG V W   TWRVG
Sbjct: 141 ILVGDRLLVANVGDSRAVICRGGNAFAVSRDHKPDQSDERERIENAGGFVMW-AGTWRVG 200

Query: 562 PAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASDGLWDVLSNSEVVSIIRDT 621
              L V+R+ GD  LK  V A+PEI E  +    ++L++ASDGLWDV SN E V+++++ 
Sbjct: 201 -GVLAVSRAFGDRLLKQYVVADPEIQEEKIDDSLEFLILASDGLWDVFSNEEAVAVVKE- 260

Query: 622 VKEPGMCSKRLATEAAARGSNDNIT 646
           V++P   +K+L  EA  RGS DNIT
Sbjct: 261 VEDPEESTKKLVGEAIKRGSADNIT 273

BLAST of MC05g1030 vs. TAIR 10
Match: AT5G10740.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 164.9 bits (416), Expect = 2.3e-40
Identity = 104/265 (39.25%), Postives = 148/265 (55.85%), Query Frame = 0

Query: 382 SSTADLAYHPILSCGSFSTCGRRETMEDSHFLLPHMCNEEDIHVFGIFDGHRGAAAAEFS 441
           +S   L+ +   S G  S+ G+R +MED         N E + +FG+FDGH GA AAE+ 
Sbjct: 21  ASGGGLSQNGKFSYGYASSAGKRSSMEDFFETRIDGINGEIVGLFGVFDGHGGARAAEYV 80

Query: 442 ARAI-PGFLRNSCSTSSPADALVETFVKTDIEFRKELDFFRQSKKVKQKDWHPGCTAVVA 501
            R +    + +    S    A+ + +  TD E  K       S+    +D   G TA  A
Sbjct: 81  KRHLFSNLITHPKFISDTKSAITDAYNHTDSELLK-------SENSHNRD--AGSTASTA 140

Query: 502 LIVRDRLFVANAGDCRTILCRAGNPIVLSKDHVASCLEERERVISAGGQVTWLVDTWRVG 561
           ++V DRL VAN GD R ++ R G  I +S+DH     +ERER+ +AGG V W   TWRVG
Sbjct: 141 ILVGDRLVVANVGDSRAVISRGGKAIAVSRDHKPDQSDERERIENAGGFVMW-AGTWRVG 200

Query: 562 PAALQVTRSIGDDDLKPAVTAEPEITETVLSSQDDYLVMASDGLWDVLSNSEVVSIIRDT 621
              L V+R+ GD  LK  V A+PEI E  +    ++L++ASDGLWDV SN   V+++++ 
Sbjct: 201 -GVLAVSRAFGDRLLKQYVVADPEIQEEKIDDTLEFLILASDGLWDVFSNEAAVAMVKE- 260

Query: 622 VKEPGMCSKRLATEAAARGSNDNIT 646
           V++P   +K+L  EA  RGS DNIT
Sbjct: 261 VEDPEDSAKKLVGEAIKRGSADNIT 273

BLAST of MC05g1030 vs. TAIR 10
Match: AT5G53140.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 163.7 bits (413), Expect = 5.2e-40
Identity = 100/255 (39.22%), Postives = 147/255 (57.65%), Query Frame = 0

Query: 393 LSCGSFSTCGRRETMEDSHFLLPHMCNEEDIHVFGIFDGHRGAAAAEF-SARAIPGFLRN 452
           LSCG  S  G+R TMED + +       + + +FGIFDGH G+ AAE+         +++
Sbjct: 100 LSCGYCSFRGKRSTMEDFYDIKASTIEGQAVCMFGIFDGHGGSRAAEYLKEHLFNNLMKH 159

Query: 453 SCSTSSPADALVETFVKTDIEF-RKELDFFRQSKKVKQKDWHPGCTAVVALIVRDRLFVA 512
               +    AL ET+ +TD+ F   E D +R            G TA  A++V + L+VA
Sbjct: 160 PQFLTDTKLALNETYKQTDVAFLESEKDTYRDD----------GSTASAAVLVGNHLYVA 219

Query: 513 NAGDCRTILCRAGNPIVLSKDHVASCLEERERVISAGGQVTWLVDTWRVGPAALQVTRSI 572
           N GD RTI+ +AG  I LS DH  +  +ER+R+ SAGG + W   TWRVG   L ++R+ 
Sbjct: 220 NVGDSRTIVSKAGKAIALSDDHKPNRSDERKRIESAGGVIMW-AGTWRVG-GVLAMSRAF 279

Query: 573 GDDDLKPAVTAEPEITETVLSSQDDYLVMASDGLWDVLSNSEVVSIIRDTVKEPGMCSKR 632
           G+  LK  V AEPEI +  +  + + LV+ASDGLWDV+ N + V++ + + +EP   +++
Sbjct: 280 GNRMLKQFVVAEPEIQDLEIDHEAELLVLASDGLWDVVPNEDAVALAQ-SEEEPEAAARK 339

Query: 633 LATEAAARGSNDNIT 646
           L   A +RGS DNIT
Sbjct: 340 LTDTAFSRGSADNIT 341