MC05g0669 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0669 vs. ExPASy Swiss-Prot
Match: Q5SP90 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Danio rerio OX=7955 GN=smek1 PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 9.0e-114
Identity = 250/714 (35.01%), Postives = 401/714 (56.16%), Query Frame = 0

Query: 11  SNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDI 70
           +++ +RVKVY LN+D +WDD+GTGHV+  Y+ER + + L V  E D   LL  +I+ +  
Sbjct: 2   TDTRRRVKVYTLNEDRQWDDRGTGHVSSAYVERLKGMSLLVRAESDGSLLLESKINPNTA 61

Query: 71  YQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQ--------RNLHFSTLSNE 130
           YQKQ+DT+I W + E + +LALSFQE  GC  IW+ IC VQ                  E
Sbjct: 62  YQKQQDTLIVWSEAE-NYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQELIDESEEE 121

Query: 131 TFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTE--LLLNDQDFFRKLMNLFRI 190
            F  ++S   ELP  +L+ L  + + +V S +   +R  +  L L ++ + RKL+ LFR+
Sbjct: 122 RFDDMSSPGLELPPCELSRLEEVAE-LVASSLPSPLRREKLALALENEGYIRKLLELFRV 181

Query: 191 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 250
           CEDL++ +GLH ++ I++GI LLN   +FE +FS+E IMD+IG LE+DP +P  +  R F
Sbjct: 182 CEDLENREGLHHLYDIIKGIFLLNRTALFEVMFSEECIMDVIGCLEFDPSLPQPRRHREF 241

Query: 251 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVL--TRILDDSMVANLNSIIHANNAI 310
           L     FKE IPI +P +  KIHQTY+V Y++D+VL    + +++M++ L+S I  N   
Sbjct: 242 LTTTARFKEVIPISDPELRQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVE 301

Query: 311 VVSLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMN 370
           +V +L+DD  F+ ELFA+L   AT DD + +LV FL EFC+ S++LQ   +   F+ L N
Sbjct: 302 IVGMLQDDEKFLTELFAQLTDEATDDDKRHELVNFLKEFCAFSQTLQPQNRDAFFKTLSN 361

Query: 371 EGIFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQ-----EGFPLFGLLVK 430
            GI   +  +L   D ++    TDI    +  +P+++R  V+++     +   L  L+++
Sbjct: 362 MGILQALEVILGMDDVQVRGAATDIFSYLVEYNPSMVREFVMQESQQNDDDILLINLIIE 421

Query: 431 GMVTDFGDNM--HCQFLEILRNLLD---TYALSGVQRDTIIEIFYEKHLGQLIDIITASC 490
            M+ D    +    Q + +LR L+D     A +     T    F+ KH   ++     + 
Sbjct: 422 HMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLSFFYKHCMHVLSAPLLAN 481

Query: 491 PTEGFQSTKKIEGAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKV 550
            TE   S    +              ++L+ I ELL FCV  H Y IK+  +  + + +V
Sbjct: 482 TTEEKPSKDDFQTC------------QLLALIVELLTFCVEHHTYHIKNYIINKDILRRV 541

Query: 551 VLLTQRREKYLVVAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSA 610
           ++LT  +  +L + A+RF+R I+   DE    Y ++N + +P+V AF+ NGSRYNL+NSA
Sbjct: 542 LVLTASQHAFLALCALRFMRKIIGLKDEFYNRYIMRNFLFEPVVKAFLNNGSRYNLINSA 601

Query: 611 VLELLEYIRKENLKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPN-- 670
           ++E+ EY+R E++K L  +II+++W  L   +   + + LK++YEQ  E  +     +  
Sbjct: 602 IIEMFEYVRVEDVKSLTAHIIENYWKGLEDVDYVQTFKGLKLRYEQQRERQDNPKLDSMR 661

Query: 671 --TLDPRKRNDERALEKEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVT 699
               + R R D R LE EEE +FN D ++ +   A V P  K+ +   L + ++
Sbjct: 662 SILRNHRFRRDARTLEDEEEMWFNTDEEDLEDGEAVVPPSDKMKNDEDLMDPIS 701

BLAST of MC05g0669 vs. ExPASy Swiss-Prot
Match: Q4S6U8 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Tetraodon nigroviridis OX=99883 GN=smek1 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.2e-113
Identity = 247/714 (34.59%), Postives = 403/714 (56.44%), Query Frame = 0

Query: 11  SNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDI 70
           +++ +RVKVY LN+D +WDD+GTGHV+  Y+ER +   L V  E D   LL  +I+ +  
Sbjct: 2   TDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERFKGTSLLVRAESDGSLLLESKINPNTA 61

Query: 71  YQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS--------NE 130
           YQKQ+DT+I W + E + +LALSFQE  GC  IW+ IC VQ       ++        +E
Sbjct: 62  YQKQQDTLIVWSEAE-NYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDVVDESEDE 121

Query: 131 TFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLL--NDQDFFRKLMNLFRI 190
            F  ++S   ELP  +L  L  + + +V S +   +R  +L L   ++ + RKL++LF +
Sbjct: 122 RFDDMSSPGLELPPCELNRLEDLAE-LVASSLPSPLRREKLALAVENEGYIRKLLDLFHV 181

Query: 191 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 250
           CEDL++ +GLH I++I++GI LLN   +FE +FSDE IMD+IG LE+DP +P  +  R F
Sbjct: 182 CEDLENREGLHHIYEIIKGIFLLNRTALFEVMFSDECIMDVIGCLEFDPALPQPRRHREF 241

Query: 251 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVL--TRILDDSMVANLNSIIHANNAI 310
           L +   FKE IPI +P +  KIHQTY+V Y++D+VL    + +++M++ L+S I  N   
Sbjct: 242 LTKTAHFKEVIPISDPELRQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVE 301

Query: 311 VVSLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMN 370
           +V +L+DD  F+ +LFA+L   AT DD + +LV FL EFC+ S++LQ   +   F+ L N
Sbjct: 302 IVGMLQDDEKFLTDLFAQLTDEATDDDKRHELVNFLKEFCAFSQTLQPQNRDAFFKTLSN 361

Query: 371 EGIFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQ-----EGFPLFGLLVK 430
            GI   +  +L   D ++    TDI    +  +P+++R  V+++     +   L  L+++
Sbjct: 362 MGILPALEVILGMDDVQVRAAATDIFSYLVEYNPSMVREFVMQESQQNDDDILLINLIIE 421

Query: 431 GMVTDFGDNM--HCQFLEILRNLLD---TYALSGVQRDTIIEIFYEKHLGQLIDIITASC 490
            M+ D    +    Q + +LR L+D     A +     T    F+ KH   ++     + 
Sbjct: 422 HMICDTDPELGGAVQLISLLRTLVDPENMLATANKTEKTEFLSFFYKHCMHVLSAPLLAN 481

Query: 491 PTEGFQSTKKIEGAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKV 550
            TE   S    + +            ++L+ I ELL FCV  H Y IK+  +  + + +V
Sbjct: 482 TTEDKPSKDDFQTS------------QLLALILELLTFCVEHHTYHIKNYIINKDILRRV 541

Query: 551 VLLTQRREKYLVVAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSA 610
           ++LT  +  +L + A+RF+R I+   DE    Y ++N + +P++ AF+ NGSRYNL+NSA
Sbjct: 542 LVLTASKHAFLALCALRFMRRIIGLKDEFYNRYIMRNFLFEPVIKAFLNNGSRYNLMNSA 601

Query: 611 VLELLEYIRKENLKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPN-- 670
           ++E+ EY+R E++K L  +I++++W  L   +   + + LK++YEQ  E  +     +  
Sbjct: 602 IIEMFEYVRVEDVKSLTAHIVENYWKALEDVDYVQTFKGLKLRYEQQRERQDNPKLDSMR 661

Query: 671 --TLDPRKRNDERALEKEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVT 699
               + R R D R LE EEE +FN D D+ +     V    K+ ++  L   ++
Sbjct: 662 SILRNHRFRRDARTLEDEEEMWFNTDEDDLEDGETVVRTSDKIKTEEDLMEPIS 701

BLAST of MC05g0669 vs. ExPASy Swiss-Prot
Match: Q6INN7 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Xenopus laevis OX=8355 GN=smek1 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.6e-113
Identity = 248/714 (34.73%), Postives = 405/714 (56.72%), Query Frame = 0

Query: 11  SNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDI 70
           +++ +RVKVY LN+D +WDD+GTGHV+  Y+ER + + L V  E D   LL  +I+++  
Sbjct: 2   TDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINTNTA 61

Query: 71  YQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS--------NE 130
           YQKQ+DT+I W + E + +LALSFQE  GC  IW+ IC VQ       ++         E
Sbjct: 62  YQKQQDTLIVWSEAE-NYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEE 121

Query: 131 TFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTEL--LLNDQDFFRKLMNLFRI 190
            F  ++S   ELP+ +L+ L  I + VV S +   +R  +L   L ++ + +KL+ LF +
Sbjct: 122 RFDDMSSPGLELPSCELSRLEEIAE-VVASSLPSPLRREKLAVALENEGYLKKLLELFHV 181

Query: 191 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 250
           CEDL++ +GLH ++++++GI LLN   +FE +FS++ +MDIIG LEYDP +P  +  R F
Sbjct: 182 CEDLENIEGLHHLYEVIKGIFLLNRTALFEVMFSEDCMMDIIGCLEYDPSLPTQRKHREF 241

Query: 251 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVL--TRILDDSMVANLNSIIHANNAI 310
           L +   FKE IPI +P +  KIHQTY+V Y++DVVL    + +++M++ L+S I  N   
Sbjct: 242 LTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDVVLPTPSVFEENMLSTLHSFIFFNKVE 301

Query: 311 VVSLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMN 370
           +V +L++D  F+ ELFA L   AT D+ +++LV FL EFC+ S++LQ   +   F+ L N
Sbjct: 302 IVGMLQEDEKFLTELFAHLTDEATDDEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSN 361

Query: 371 EGIFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQ-----EGFPLFGLLVK 430
            GI   +  +L   D ++    TDI    +  +P+++R  V+++     +   L  L+++
Sbjct: 362 MGILPALEVILGMDDPQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIE 421

Query: 431 GMVTDFGDNM--HCQFLEILRNLLD---TYALSGVQRDTIIEIFYEKHLGQLIDIITASC 490
            M+ D    +    Q   +LR L+D     A +     T    F+ KH   ++     + 
Sbjct: 422 HMICDTDPELGGAVQLTGLLRTLVDPENMLATTNKTEKTEFLGFFYKHCMHVLTAPLLAN 481

Query: 491 PTEGFQSTKKIEGAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKV 550
            TE   S    + A            ++L+ I ELL FCV  H Y IK+  +  + + +V
Sbjct: 482 TTEEKPSKDDFQAA------------QLLALILELLTFCVEHHTYHIKNYIINKDILRRV 541

Query: 551 VLLTQRREKYLVVAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSA 610
           ++L      +L + A+RF+R I+   DE    Y VK+ + +P+V AF+ NGSRYNL+NSA
Sbjct: 542 LVLMSSMHSFLGLCALRFMRKIVGLKDEFYNRYIVKSFLFEPVVKAFLNNGSRYNLMNSA 601

Query: 611 VLELLEYIRKENLKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPN-- 670
           +LE+ EYIR E++K L  ++++++W  L   E   + + LK++Y+Q  E  +     +  
Sbjct: 602 ILEIFEYIRVEDIKSLTAHVVENYWKALEDVEYVQTFKGLKLRYDQQRERQDNPKLDSMR 661

Query: 671 --TLDPRKRNDERALEKEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVT 699
               + R R D R++E EEE +FN D D+ +     V P +K+ +   L + ++
Sbjct: 662 SILRNHRYRRDARSIEDEEEMWFNTDEDDLEDGEPVVLPSEKIKNSEDLMDPIS 701

BLAST of MC05g0669 vs. ExPASy Swiss-Prot
Match: Q6DFT3 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Xenopus tropicalis OX=8364 GN=ppp4r3b PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.2e-112
Identity = 254/718 (35.38%), Postives = 405/718 (56.41%), Query Frame = 0

Query: 11  SNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDI 70
           S++ +RVKVY LN+D +WDD+GTGHV+  Y+ER + + L V  E D   LL  +I+ +  
Sbjct: 2   SDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVERLKGMSLLVRAESDGSLLLESKINPNTA 61

Query: 71  YQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQ--------RNLHFSTLSNE 130
           YQKQ+DT+I W + E + +LALSFQE  GC  IW+ IC VQ                  E
Sbjct: 62  YQKQQDTLIVWSEAE-NYDLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDPIDESEEE 121

Query: 131 TFHSV--NSELRELPAVDLTTLPLIHKTVVESGIADQMRLTE--LLLNDQDFFRKLMNLF 190
            F  +   S L +LP  +L+ L  I   +V S ++  +R  +  L L ++ + +KL+ LF
Sbjct: 122 RFEEMPETSNLIDLPTCELSKLEEI-ADLVTSVLSSPIRREKLALALENEGYIKKLLQLF 181

Query: 191 RICEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFR 250
           + CE+LD+T+GLH +++I+RGI+ LN   +FE +FSDE IMD++G LEYDP +   +  R
Sbjct: 182 QTCENLDNTEGLHHLYEIIRGILFLNKAALFEVMFSDECIMDVVGCLEYDPALAQPKRHR 241

Query: 251 NFLKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVL--TRILDDSMVANLNSIIHANN 310
            FL +   FKE IPI +  +  KIHQTY+V Y++DV+L    + +++ ++ L S I  N 
Sbjct: 242 EFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDVILPTPSVFEENFLSTLTSFIFFNK 301

Query: 311 AIVVSLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDL 370
             +VS+L++D  F+ E+FA+L   AT DD +++LV F  EFC+ S++LQ   +   F+ L
Sbjct: 302 VEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQNRDAFFKTL 361

Query: 371 MNEGIFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQ-----EGFPLFGLL 430
            N GI   +  V+   D ++    TDI    +   P+++R  V+++     +   L  ++
Sbjct: 362 ANLGILPALEIVMGMDDLQVRAAATDIFSYLVEFSPSMVREFVMQEAQQSDDDILLINVV 421

Query: 431 VKGMVTDFGDNM--HCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCP 490
           ++ M+ D    +    Q + +LR L+D   +      T    F        + ++TA  P
Sbjct: 422 IEQMICDSDPELGGAVQLMGLLRTLIDPENMLATANKTEKSEFLNFFYNHCMHVLTA--P 481

Query: 491 TEGFQSTKKIEGAGVILGD--TNSVKP------EILSNICELLCFCVLQHPYRIKSNFLL 550
                S  K+E    +LG   T++V P      ++L+ I ELL FCV  H Y IK+  + 
Sbjct: 482 LLANTSEDKLE-KDAVLGSIKTSTVCPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMN 541

Query: 551 NNTIDKVVLLTQRREKYLVVAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSR 610
            + + +V++L   +  +L + A+RF+R I+   DE    Y +K N+ +P+++A + NG+R
Sbjct: 542 KDLLRRVLILMNSKHTFLALCALRFMRRIIGLKDEFYNRYIIKGNLFEPVINALLDNGTR 601

Query: 611 YNLLNSAVLELLEYIRKENLKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCET 670
           YNLLNSA++EL E+IR E++K L  +I+++F+  L   E   + + LK +YEQ  +    
Sbjct: 602 YNLLNSAIIELFEFIRVEDIKSLTSHIVENFYKALESIEYVQTFKGLKTRYEQEKDRQSQ 661

Query: 671 K--GTPNTL-DPRKRNDERALEKEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNG 697
           K    P+ L   R R D RALE +EE +FNED +E+    A V P +K  ++     G
Sbjct: 662 KLSSVPSILRSNRFRRDARALEDDEELWFNEDEEEE--GEAVVPPVEKTKTEDDFPEG 712

BLAST of MC05g0669 vs. ExPASy Swiss-Prot
Match: Q922R5 (Serine/threonine-protein phosphatase 4 regulatory subunit 3B OS=Mus musculus OX=10090 GN=Ppp4r3b PE=1 SV=2)

HSP 1 Score: 405.6 bits (1041), Expect = 1.4e-111
Identity = 250/708 (35.31%), Postives = 402/708 (56.78%), Query Frame = 0

Query: 11  SNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDI 70
           S++ +RVKVY LN+D +WDD+GTGHV+  Y+E  + + L V  E D   LL  +I+ +  
Sbjct: 2   SDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVEELKGMSLLVRAESDGSLLLESKINPNTA 61

Query: 71  YQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQ---------RNLHFSTLSN 130
           YQKQ+DT+I W + E + +LALSFQE  GC  IW+ IC VQ         ++L       
Sbjct: 62  YQKQQDTLIVWSEAE-NYDLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDL-IDESEE 121

Query: 131 ETFHSV--NSELRELPAVDLTTLPLIHKTVVESGIADQMRLTE--LLLNDQDFFRKLMNL 190
           E F  +   S L +LPA +L+ L  I   +V S ++  +R  +  L L ++ + +KL+ L
Sbjct: 122 ERFEEMPETSHLIDLPACELSKLEEI-ADLVTSVLSSPIRREKLALALENEGYIKKLLQL 181

Query: 191 FRICEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHF 250
           F+ CE+L++T+GLH +++I+RGI+ LN   +FE +FSDE IMD++G LEYDP +   +  
Sbjct: 182 FQACENLENTEGLHHLYEIIRGILFLNKATLFEVMFSDECIMDVVGCLEYDPALAQPKRH 241

Query: 251 RNFLKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVL--TRILDDSMVANLNSIIHAN 310
           R FL +   FKE IPI +  +  KIHQTY+V Y++D++L    + +++ ++ L S I  N
Sbjct: 242 REFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDIILPTPSVFEENFLSTLTSFIFFN 301

Query: 311 NAIVVSLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRD 370
              +VS+L++D  F+ E+FA+L   AT DD +++LV F  EFC+ S++LQ   +   F+ 
Sbjct: 302 KVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQNRDAFFKT 361

Query: 371 LMNEGIFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQ-----EGFPLFGL 430
           L   GI   +  V+   D ++    TDI    +   P+++R  V+++     +   L  +
Sbjct: 362 LAKLGILPALEIVMGMDDLQVRSAATDIFSYLVEFSPSMVREFVMQEAQQSDDDVLLINV 421

Query: 431 LVKGMVTDFGDNM--HCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASC 490
           +++ M+ D    +    Q + +LR L+D   +      T    F        + ++TA  
Sbjct: 422 VIEQMICDTDPELGGAVQLMGLLRTLIDPENMLATTNKTEKSEFLNFFYNHCMHVLTAPL 481

Query: 491 PTEGFQSTKKIEGAGVI-LGDTNSVKP------EILSNICELLCFCVLQHPYRIKSNFLL 550
            T    S  K E   ++    TN++ P      ++L+ I ELL FCV  H Y IK+  + 
Sbjct: 482 LTN--TSEDKCEKDNMLGSNTTNTICPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMN 541

Query: 551 NNTIDKVVLLTQRREKYLVVAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSR 610
            + + +V++L   +  +L + A+RF+R I+   DE    Y  K N+ +P+++A + NG+R
Sbjct: 542 KDLLRRVLVLMNSKHTFLALCALRFMRRIIGLKDEFYNRYITKGNLFEPVINALLDNGTR 601

Query: 611 YNLLNSAVLELLEYIRKENLKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQ--CLENC 670
           YNLLNSAV+EL E+IR E++K L  +I+++F+  L   E   + + LK KYEQ    +N 
Sbjct: 602 YNLLNSAVIELFEFIRVEDIKSLTAHIVENFYKALESIEYVQTFKGLKTKYEQEKDRQNQ 661

Query: 671 ETKGTPNTL-DPRKRNDERALEKEEEDYFNEDSDEDDTASASVAPKQK 687
           +    P+ L   R R D +ALE++EE +FNED DE+  A  +   K K
Sbjct: 662 KLNSVPSILRSNRFRRDAKALEEDEEMWFNEDDDEEGKAVITPVEKSK 704

BLAST of MC05g0669 vs. NCBI nr
Match: XP_022138258.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X1 [Momordica charantia])

HSP 1 Score: 1721 bits (4456), Expect = 0.0
Identity = 886/886 (100.00%), Postives = 886/886 (100.00%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS
Sbjct: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI
Sbjct: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF
Sbjct: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG
Sbjct: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV
Sbjct: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN
Sbjct: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE
Sbjct: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD
Sbjct: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS
Sbjct: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780

Query: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840
           TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK
Sbjct: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840

Query: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS
Sbjct: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886

BLAST of MC05g0669 vs. NCBI nr
Match: XP_022138259.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X2 [Momordica charantia])

HSP 1 Score: 1694 bits (4388), Expect = 0.0
Identity = 871/873 (99.77%), Postives = 873/873 (100.00%), Query Frame = 0

Query: 14  MQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYQK 73
           ++RVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYQK
Sbjct: 12  LKRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYQK 71

Query: 74  QEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLSNETFHSVNSELRE 133
           QEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLSNETFHSVNSELRE
Sbjct: 72  QEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLSNETFHSVNSELRE 131

Query: 134 LPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRICEDLDDTDGLHMI 193
           LPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRICEDLDDTDGLHMI
Sbjct: 132 LPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRICEDLDDTDGLHMI 191

Query: 194 FKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNFLKEHVVFKEAIPI 253
           FKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNFLKEHVVFKEAIPI
Sbjct: 192 FKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNFLKEHVVFKEAIPI 251

Query: 254 KNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVVSLLKDDNTFIQEL 313
           KNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVVSLLKDDNTFIQEL
Sbjct: 252 KNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVVSLLKDDNTFIQEL 311

Query: 314 FARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSGD 373
           FARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSGD
Sbjct: 312 FARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSGD 371

Query: 374 EKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRN 433
           EKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRN
Sbjct: 372 EKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRN 431

Query: 434 LLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIEGAGVILGDTNSVK 493
           LLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIEGAGVILGDTNSVK
Sbjct: 432 LLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIEGAGVILGDTNSVK 491

Query: 494 PEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLVVAAVRFIRTILSR 553
           PEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLVVAAVRFIRTILSR
Sbjct: 492 PEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLVVAAVRFIRTILSR 551

Query: 554 HDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKENLKLLVRYIIDSFW 613
           HDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKENLKLLVRYIIDSFW
Sbjct: 552 HDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKENLKLLVRYIIDSFW 611

Query: 614 NQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALEKEEEDYFNEDSDE 673
           NQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALEKEEEDYFNEDSDE
Sbjct: 612 NQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALEKEEEDYFNEDSDE 671

Query: 674 DDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDDEDYKPPQRKQPDT 733
           DDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDDEDYKPPQRKQPDT
Sbjct: 672 DDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDDEDYKPPQRKQPDT 731

Query: 734 SDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCSTLSQAVLPGKKTM 793
           SDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCSTLSQAVLPGKKTM
Sbjct: 732 SDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCSTLSQAVLPGKKTM 791

Query: 794 NASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREKESAASRSYSSSSV 853
           NASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREKESAASRSYSSSSV
Sbjct: 792 NASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREKESAASRSYSSSSV 851

Query: 854 LHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           LHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS
Sbjct: 852 LHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 884

BLAST of MC05g0669 vs. NCBI nr
Match: XP_022979704.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3A-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1511 bits (3913), Expect = 0.0
Identity = 783/886 (88.37%), Postives = 825/886 (93.12%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIY+KQEDTIISW+DPEYSTELALSFQEATGCSFIWD+ICSVQRNLHFS LS
Sbjct: 61  LLHRISSDDIYRKQEDTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           NE FHSVNSELRELPAV+L+TLPLI KTVVESGIADQMRLTELLLNDQDFFRKL NLFR+
Sbjct: 121 NEAFHSVNSELRELPAVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRV 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIFKI RGIVLLNS QIFEKIFSDE+IMDIIGSLEYDPEVPH Q FRNF
Sbjct: 181 CEDLENIDGLHMIFKIFRGIVLLNSQQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTY+VGYLKDVVL R++DDSMVA+LNSIIHANNA VV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           + LKDD+TFIQELFARLRSP+T DDSKKDLVYFLHEFC LSKSLQMV QLRLFRDLMNEG
Sbjct: 301 TSLKDDSTFIQELFARLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQS D+K VLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSQDKKFVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+S KKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GA   LGD N VKPEILSNICELLCFCVLQHPYRIKSNFLLNN IDKVVLLTQRREKYLV
Sbjct: 481 GAVEGLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRF+RT+LSRHDEHLINYFVKN+ LKPI+DAFVANG+RYNLLNS VL+LLE+IR EN
Sbjct: 541 VAAVRFVRTVLSRHDEHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLL+RYIIDSFWNQLSQFEN  SIQALKVKYEQCLEN E KGT  T DPRKRNDERALE
Sbjct: 601 LKLLIRYIIDSFWNQLSQFENLPSIQALKVKYEQCLENYEMKGTLETSDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDE DTASA  APKQ V SQPALSNG T +YP  S RSGGLVDYDDD+DD
Sbjct: 661 KEEEDYFNEDSDEVDTASA--APKQNVQSQPALSNGGTTNYPLVSLRSGGLVDYDDDEDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDY+PPQR QPD+SDEDNEMME+L +KRKLAPQEEP+M KKQ L KK+KPKDGV ASLCS
Sbjct: 721 EDYRPPQRDQPDSSDEDNEMMESLGQKRKLAPQEEPQMIKKQLLSKKYKPKDGVLASLCS 780

Query: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840
           TLSQA+LPGKKT NA+ TSPCT YENKSS E NH+T PAISSSSSDSS+ NSDEEN REK
Sbjct: 781 TLSQAILPGKKTTNATQTSPCTPYENKSSGEANHETGPAISSSSSDSSSSNSDEENRREK 840

Query: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           ES ASRSY +SS+LHITSENRQLGGDD+PLIPPKSSPEMTVNGSLS
Sbjct: 841 ESTASRSYPNSSILHITSENRQLGGDDHPLIPPKSSPEMTVNGSLS 884

BLAST of MC05g0669 vs. NCBI nr
Match: XP_023529074.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1510 bits (3910), Expect = 0.0
Identity = 780/886 (88.04%), Postives = 826/886 (93.23%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIY+KQEDTIISW+DPEYSTELALSFQEATGCSFIWD+ICSVQRNLHFS LS
Sbjct: 61  LLHRISSDDIYRKQEDTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           +E FHSVNSELRELPAV+L+TLPLI KTVVESGIADQMRLTELLLNDQDFFRKL NLFR+
Sbjct: 121 SEAFHSVNSELRELPAVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRV 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIFKI RGIVLLNSPQIFEKIFSDE+IMDIIGSLEYDPEVPH Q FRNF
Sbjct: 181 CEDLENIDGLHMIFKIFRGIVLLNSPQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTY+VGYLKDVVL R++DDSMVA+LNSIIHANNA VV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           + LKDD+TFIQELFARLRSP+T DDSKKDLVYFLHEFC LSKSLQMV QLRLFRDLMNEG
Sbjct: 301 TSLKDDSTFIQELFARLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQS D+K VLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSQDKKFVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+S KKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GA   LGD N VKPEILSNICELLCFCVLQHPYRIKSNFLLNN IDKVVLLTQRREKYLV
Sbjct: 481 GAVEGLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRF+RT+LSRHDEHLINYFVKN+ LKPI+DAF+ANG+RYNLLNS VL+LLE+IR EN
Sbjct: 541 VAAVRFVRTVLSRHDEHLINYFVKNSTLKPIIDAFIANGNRYNLLNSVVLDLLEFIRTEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLL+RYIIDSFWNQLSQFEN  SIQALKVKYEQCLEN E KGT  T DPRKRNDERALE
Sbjct: 601 LKLLIRYIIDSFWNQLSQFENLPSIQALKVKYEQCLENYEMKGTLETSDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDE+DTASA  APKQ V SQPAL NGVT +YP  S RSGGLVDYDDD+DD
Sbjct: 661 KEEEDYFNEDSDEEDTASA--APKQNVQSQPALCNGVTTNYPLVSLRSGGLVDYDDDEDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDY+PPQR QPD+SDEDNEMME+L +KRKLAPQEEP+M KKQQL KK+KPKDGV ASLC+
Sbjct: 721 EDYRPPQRDQPDSSDEDNEMMESLGQKRKLAPQEEPQMVKKQQLSKKYKPKDGVLASLCT 780

Query: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840
           TLSQA+LPGKKT NA+ TS CT YENKSS E NH+T PAISSSSSDSS+ NSDEEN REK
Sbjct: 781 TLSQAILPGKKTTNATQTSLCTPYENKSSGEANHETSPAISSSSSDSSSSNSDEENRREK 840

Query: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           ES ASRSY +SS+LHITSENRQLGGDD+PLIPPKSS EMTVNGSLS
Sbjct: 841 ESTASRSYPNSSILHITSENRQLGGDDHPLIPPKSSTEMTVNGSLS 884

BLAST of MC05g0669 vs. NCBI nr
Match: XP_022955486.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1509 bits (3906), Expect = 0.0
Identity = 783/888 (88.18%), Postives = 826/888 (93.02%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIY+KQEDTIISW+DPEYSTELALSFQEATGCSFIWD+ICSVQRNLHFS LS
Sbjct: 61  LLHRISSDDIYRKQEDTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           NE FHSVNSELRELPAV+L+TLPLI KTVVESGIADQMRLTELLLNDQDFFRKL NLFR+
Sbjct: 121 NEAFHSVNSELRELPAVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRV 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIFKI RGIVLLNSPQIFEKIFSDE+IMDIIGSLEYDPEVPH Q FRNF
Sbjct: 181 CEDLENIDGLHMIFKIFRGIVLLNSPQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTY+VGYLKDVVL R++DDSMVA+LNSIIHANNA VV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           + LKDD+TFIQELFARLRSP+T DDSKKDLVYFLHEFC LSKSLQMV QLRLFRDLMNEG
Sbjct: 301 TSLKDDSTFIQELFARLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQS D+K VLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSQDKKFVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+S KKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GA   LGD N VKPEILSNICELLCFCVLQHPYRIKSNFLLNN IDKVVLLTQRREKYLV
Sbjct: 481 GAVEGLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRF+RTILSRHDEHLINYFVKN+ LKPI+DAFVANG+RYNLLNS VL+LLE+IR EN
Sbjct: 541 VAAVRFVRTILSRHDEHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLL+RYIIDSFWNQLSQFEN  SIQALKVKYEQCLEN E KG   T DPRKRNDERALE
Sbjct: 601 LKLLIRYIIDSFWNQLSQFENLPSIQALKVKYEQCLENYEMKGALETSDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDE+DTASA  APKQ V SQPAL NGVT +YP  S RSGGLVDYDDD+DD
Sbjct: 661 KEEEDYFNEDSDEEDTASA--APKQNVQSQPALCNGVTTNYPLVSLRSGGLVDYDDDEDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDY+PPQR QPD+SDEDNEMME+L +KRKLAPQEEP+M KKQ L KK+KPKDGV ASLC+
Sbjct: 721 EDYRPPQRDQPDSSDEDNEMMESLGQKRKLAPQEEPQMVKKQLLSKKYKPKDGVLASLCT 780

Query: 781 TLSQAVLPGKKTMNAS--HTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHR 840
           TLSQA+LPGKKT NA+   TS CT YENKSS EENH+T PAISSSSSDSS+ NSDEEN R
Sbjct: 781 TLSQAILPGKKTTNATPTQTSLCTPYENKSSGEENHETSPAISSSSSDSSSSNSDEENRR 840

Query: 841 EKESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           EKES ASRSY +SS+LHITSENRQLGGDD+PLIPPKSSPEMTVNGSLS
Sbjct: 841 EKESTASRSYPNSSILHITSENRQLGGDDHPLIPPKSSPEMTVNGSLS 886

BLAST of MC05g0669 vs. ExPASy TrEMBL
Match: A0A6J1CCJ6 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009477 PE=4 SV=1)

HSP 1 Score: 1721 bits (4456), Expect = 0.0
Identity = 886/886 (100.00%), Postives = 886/886 (100.00%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS
Sbjct: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI
Sbjct: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF
Sbjct: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG
Sbjct: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV
Sbjct: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN
Sbjct: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE
Sbjct: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD
Sbjct: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS
Sbjct: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780

Query: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840
           TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK
Sbjct: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840

Query: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS
Sbjct: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886

BLAST of MC05g0669 vs. ExPASy TrEMBL
Match: A0A6J1C9M8 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009477 PE=4 SV=1)

HSP 1 Score: 1694 bits (4388), Expect = 0.0
Identity = 871/873 (99.77%), Postives = 873/873 (100.00%), Query Frame = 0

Query: 14  MQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYQK 73
           ++RVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYQK
Sbjct: 12  LKRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYQK 71

Query: 74  QEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLSNETFHSVNSELRE 133
           QEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLSNETFHSVNSELRE
Sbjct: 72  QEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLSNETFHSVNSELRE 131

Query: 134 LPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRICEDLDDTDGLHMI 193
           LPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRICEDLDDTDGLHMI
Sbjct: 132 LPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRICEDLDDTDGLHMI 191

Query: 194 FKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNFLKEHVVFKEAIPI 253
           FKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNFLKEHVVFKEAIPI
Sbjct: 192 FKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNFLKEHVVFKEAIPI 251

Query: 254 KNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVVSLLKDDNTFIQEL 313
           KNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVVSLLKDDNTFIQEL
Sbjct: 252 KNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVVSLLKDDNTFIQEL 311

Query: 314 FARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSGD 373
           FARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSGD
Sbjct: 312 FARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSGD 371

Query: 374 EKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRN 433
           EKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRN
Sbjct: 372 EKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRN 431

Query: 434 LLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIEGAGVILGDTNSVK 493
           LLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIEGAGVILGDTNSVK
Sbjct: 432 LLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIEGAGVILGDTNSVK 491

Query: 494 PEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLVVAAVRFIRTILSR 553
           PEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLVVAAVRFIRTILSR
Sbjct: 492 PEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLVVAAVRFIRTILSR 551

Query: 554 HDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKENLKLLVRYIIDSFW 613
           HDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKENLKLLVRYIIDSFW
Sbjct: 552 HDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKENLKLLVRYIIDSFW 611

Query: 614 NQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALEKEEEDYFNEDSDE 673
           NQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALEKEEEDYFNEDSDE
Sbjct: 612 NQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALEKEEEDYFNEDSDE 671

Query: 674 DDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDDEDYKPPQRKQPDT 733
           DDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDDEDYKPPQRKQPDT
Sbjct: 672 DDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDDEDYKPPQRKQPDT 731

Query: 734 SDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCSTLSQAVLPGKKTM 793
           SDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCSTLSQAVLPGKKTM
Sbjct: 732 SDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCSTLSQAVLPGKKTM 791

Query: 794 NASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREKESAASRSYSSSSV 853
           NASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREKESAASRSYSSSSV
Sbjct: 792 NASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREKESAASRSYSSSSV 851

Query: 854 LHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           LHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS
Sbjct: 852 LHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 884

BLAST of MC05g0669 vs. ExPASy TrEMBL
Match: A0A6J1IXB5 (serine/threonine-protein phosphatase 4 regulatory subunit 3A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479339 PE=4 SV=1)

HSP 1 Score: 1511 bits (3913), Expect = 0.0
Identity = 783/886 (88.37%), Postives = 825/886 (93.12%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIY+KQEDTIISW+DPEYSTELALSFQEATGCSFIWD+ICSVQRNLHFS LS
Sbjct: 61  LLHRISSDDIYRKQEDTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           NE FHSVNSELRELPAV+L+TLPLI KTVVESGIADQMRLTELLLNDQDFFRKL NLFR+
Sbjct: 121 NEAFHSVNSELRELPAVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRV 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIFKI RGIVLLNS QIFEKIFSDE+IMDIIGSLEYDPEVPH Q FRNF
Sbjct: 181 CEDLENIDGLHMIFKIFRGIVLLNSQQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTY+VGYLKDVVL R++DDSMVA+LNSIIHANNA VV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           + LKDD+TFIQELFARLRSP+T DDSKKDLVYFLHEFC LSKSLQMV QLRLFRDLMNEG
Sbjct: 301 TSLKDDSTFIQELFARLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQS D+K VLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSQDKKFVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+S KKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GA   LGD N VKPEILSNICELLCFCVLQHPYRIKSNFLLNN IDKVVLLTQRREKYLV
Sbjct: 481 GAVEGLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRF+RT+LSRHDEHLINYFVKN+ LKPI+DAFVANG+RYNLLNS VL+LLE+IR EN
Sbjct: 541 VAAVRFVRTVLSRHDEHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLL+RYIIDSFWNQLSQFEN  SIQALKVKYEQCLEN E KGT  T DPRKRNDERALE
Sbjct: 601 LKLLIRYIIDSFWNQLSQFENLPSIQALKVKYEQCLENYEMKGTLETSDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDE DTASA  APKQ V SQPALSNG T +YP  S RSGGLVDYDDD+DD
Sbjct: 661 KEEEDYFNEDSDEVDTASA--APKQNVQSQPALSNGGTTNYPLVSLRSGGLVDYDDDEDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDY+PPQR QPD+SDEDNEMME+L +KRKLAPQEEP+M KKQ L KK+KPKDGV ASLCS
Sbjct: 721 EDYRPPQRDQPDSSDEDNEMMESLGQKRKLAPQEEPQMIKKQLLSKKYKPKDGVLASLCS 780

Query: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840
           TLSQA+LPGKKT NA+ TSPCT YENKSS E NH+T PAISSSSSDSS+ NSDEEN REK
Sbjct: 781 TLSQAILPGKKTTNATQTSPCTPYENKSSGEANHETGPAISSSSSDSSSSNSDEENRREK 840

Query: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           ES ASRSY +SS+LHITSENRQLGGDD+PLIPPKSSPEMTVNGSLS
Sbjct: 841 ESTASRSYPNSSILHITSENRQLGGDDHPLIPPKSSPEMTVNGSLS 884

BLAST of MC05g0669 vs. ExPASy TrEMBL
Match: A0A6J1GTW5 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457499 PE=4 SV=1)

HSP 1 Score: 1509 bits (3906), Expect = 0.0
Identity = 783/888 (88.18%), Postives = 826/888 (93.02%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIY+KQEDTIISW+DPEYSTELALSFQEATGCSFIWD+ICSVQRNLHFS LS
Sbjct: 61  LLHRISSDDIYRKQEDTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           NE FHSVNSELRELPAV+L+TLPLI KTVVESGIADQMRLTELLLNDQDFFRKL NLFR+
Sbjct: 121 NEAFHSVNSELRELPAVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRV 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIFKI RGIVLLNSPQIFEKIFSDE+IMDIIGSLEYDPEVPH Q FRNF
Sbjct: 181 CEDLENIDGLHMIFKIFRGIVLLNSPQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTY+VGYLKDVVL R++DDSMVA+LNSIIHANNA VV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           + LKDD+TFIQELFARLRSP+T DDSKKDLVYFLHEFC LSKSLQMV QLRLFRDLMNEG
Sbjct: 301 TSLKDDSTFIQELFARLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQS D+K VLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSQDKKFVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+S KKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GA   LGD N VKPEILSNICELLCFCVLQHPYRIKSNFLLNN IDKVVLLTQRREKYLV
Sbjct: 481 GAVEGLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRF+RTILSRHDEHLINYFVKN+ LKPI+DAFVANG+RYNLLNS VL+LLE+IR EN
Sbjct: 541 VAAVRFVRTILSRHDEHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLL+RYIIDSFWNQLSQFEN  SIQALKVKYEQCLEN E KG   T DPRKRNDERALE
Sbjct: 601 LKLLIRYIIDSFWNQLSQFENLPSIQALKVKYEQCLENYEMKGALETSDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDE+DTASA  APKQ V SQPAL NGVT +YP  S RSGGLVDYDDD+DD
Sbjct: 661 KEEEDYFNEDSDEEDTASA--APKQNVQSQPALCNGVTTNYPLVSLRSGGLVDYDDDEDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDY+PPQR QPD+SDEDNEMME+L +KRKLAPQEEP+M KKQ L KK+KPKDGV ASLC+
Sbjct: 721 EDYRPPQRDQPDSSDEDNEMMESLGQKRKLAPQEEPQMVKKQLLSKKYKPKDGVLASLCT 780

Query: 781 TLSQAVLPGKKTMNAS--HTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHR 840
           TLSQA+LPGKKT NA+   TS CT YENKSS EENH+T PAISSSSSDSS+ NSDEEN R
Sbjct: 781 TLSQAILPGKKTTNATPTQTSLCTPYENKSSGEENHETSPAISSSSSDSSSSNSDEENRR 840

Query: 841 EKESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           EKES ASRSY +SS+LHITSENRQLGGDD+PLIPPKSSPEMTVNGSLS
Sbjct: 841 EKESTASRSYPNSSILHITSENRQLGGDDHPLIPPKSSPEMTVNGSLS 886

BLAST of MC05g0669 vs. ExPASy TrEMBL
Match: A0A6J1IU29 (serine/threonine-protein phosphatase 4 regulatory subunit 3A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479339 PE=4 SV=1)

HSP 1 Score: 1505 bits (3896), Expect = 0.0
Identity = 782/886 (88.26%), Postives = 824/886 (93.00%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL
Sbjct: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           LLHRISSDDIY+KQEDTIISW+DPEYSTELALSFQEATGCSFIWD+ICSVQRNLHFS LS
Sbjct: 61  LLHRISSDDIYRKQEDTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLS 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           NE FHSVNSELRELPAV+L+TLPLI KTVVESGIADQMRLTELLLND DFFRKL NLFR+
Sbjct: 121 NEAFHSVNSELRELPAVELSTLPLILKTVVESGIADQMRLTELLLND-DFFRKLTNLFRV 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIFKI RGIVLLNS QIFEKIFSDE+IMDIIGSLEYDPEVPH Q FRNF
Sbjct: 181 CEDLENIDGLHMIFKIFRGIVLLNSQQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIKNPLVLSKIHQTY+VGYLKDVVL R++DDSMVA+LNSIIHANNA VV
Sbjct: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           + LKDD+TFIQELFARLRSP+T DDSKKDLVYFLHEFC LSKSLQMV QLRLFRDLMNEG
Sbjct: 301 TSLKDDSTFIQELFARLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IFDIITDVLQS D+K VLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG
Sbjct: 361 IFDIITDVLQSQDKKFVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKIE 480
           DNMHCQFLEILRNLLDTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+S KKIE
Sbjct: 421 DNMHCQFLEILRNLLDTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIE 480

Query: 481 GAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLV 540
           GA   LGD N VKPEILSNICELLCFCVLQHPYRIKSNFLLNN IDKVVLLTQRREKYLV
Sbjct: 481 GAVEGLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLV 540

Query: 541 VAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKEN 600
           VAAVRF+RT+LSRHDEHLINYFVKN+ LKPI+DAFVANG+RYNLLNS VL+LLE+IR EN
Sbjct: 541 VAAVRFVRTVLSRHDEHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTEN 600

Query: 601 LKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERALE 660
           LKLL+RYIIDSFWNQLSQFEN  SIQALKVKYEQCLEN E KGT  T DPRKRNDERALE
Sbjct: 601 LKLLIRYIIDSFWNQLSQFENLPSIQALKVKYEQCLENYEMKGTLETSDPRKRNDERALE 660

Query: 661 KEEEDYFNEDSDEDDTASASVAPKQKVPSQPALSNGVTISYPPASPRSGGLVDYDDDDDD 720
           KEEEDYFNEDSDE DTASA  APKQ V SQPALSNG T +YP  S RSGGLVDYDDD+DD
Sbjct: 661 KEEEDYFNEDSDEVDTASA--APKQNVQSQPALSNGGTTNYPLVSLRSGGLVDYDDDEDD 720

Query: 721 EDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPKDGVFASLCS 780
           EDY+PPQR QPD+SDEDNEMME+L +KRKLAPQEEP+M KKQ L KK+KPKDGV ASLCS
Sbjct: 721 EDYRPPQRDQPDSSDEDNEMMESLGQKRKLAPQEEPQMIKKQLLSKKYKPKDGVLASLCS 780

Query: 781 TLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCNSDEENHREK 840
           TLSQA+LPGKKT NA+ TSPCT YENKSS E NH+T PAISSSSSDSS+ NSDEEN REK
Sbjct: 781 TLSQAILPGKKTTNATQTSPCTPYENKSSGEANHETGPAISSSSSDSSSSNSDEENRREK 840

Query: 841 ESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGSLS 886
           ES ASRSY +SS+LHITSENRQLGGDD+PLIPPKSSPEMTVNGSLS
Sbjct: 841 ESTASRSYPNSSILHITSENRQLGGDDHPLIPPKSSPEMTVNGSLS 883

BLAST of MC05g0669 vs. TAIR 10
Match: AT3G06670.1 (binding )

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 586/893 (65.62%), Postives = 717/893 (80.29%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGA E+SQ+N+NSMQRVKVY LN+DGKWDD+GTGHV++D++ERSEEL L VIDEEDNETL
Sbjct: 1   MGAPEKSQSNTNSMQRVKVYHLNEDGKWDDRGTGHVSIDFVERSEELSLCVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           L+H I+ +DIY+KQEDTIISW+DPE STELALSFQE  GCS++WD IC++QRNLHFS+L+
Sbjct: 61  LVHPINPEDIYRKQEDTIISWRDPERSTELALSFQETAGCSYVWDQICTMQRNLHFSSLN 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           +ETFHS+NSELRELPAV+LTTLPLI K V ESGI DQMRLTEL+L D DFFR LM +F+I
Sbjct: 121 SETFHSLNSELRELPAVELTTLPLILKIVTESGITDQMRLTELILKDHDFFRNLMGVFKI 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIF IV+GI+LLNS QI EKIF DE+IM+IIG LEYDP VPH+QH RNF
Sbjct: 181 CEDLENVDGLHMIFNIVKGIILLNSSQILEKIFGDELIMEIIGCLEYDPGVPHSQHHRNF 240

Query: 241 LKEHVVFKEAIPIKNPLVLSKIHQTYKVGYLKDVVLTRILDDSMVANLNSIIHANNAIVV 300
           LKEHVVFKEAIPIK+PLVLSKIHQTY++GYLKDVVL R+LDD++VANLNS+IHANNAIVV
Sbjct: 241 LKEHVVFKEAIPIKDPLVLSKIHQTYRIGYLKDVVLARVLDDAIVANLNSVIHANNAIVV 300

Query: 301 SLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCSLSKSLQMVQQLRLFRDLMNEG 360
           SLLKDD+TFIQELFARLRSP+T  +SKK+LVYFLHEFCSLSKSLQ+VQQLRLFRDL+NEG
Sbjct: 301 SLLKDDSTFIQELFARLRSPSTSMESKKNLVYFLHEFCSLSKSLQVVQQLRLFRDLINEG 360

Query: 361 IFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFG 420
           IF +I +VLQ  D+KLVLTGTDILILFL QDPNLLR++VVR EG PL GLLVKGM+ DFG
Sbjct: 361 IFHVIEEVLQIPDKKLVLTGTDILILFLTQDPNLLRSYVVRTEGNPLLGLLVKGMMEDFG 420

Query: 421 DNMHCQFLEILRNLLDTYALS-GVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSTKKI 480
           D MHCQFLEI+R LLD  ALS G QR  I++IFYEKHL +L+D+ITASCP    +S+   
Sbjct: 421 DKMHCQFLEIIRTLLDANALSGGAQRANIMDIFYEKHLPELVDVITASCPE---KSSNAS 480

Query: 481 EGAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYL 540
           EGA   +      KPE+L NICELLCFC++Q   R K +FL NN  +KV+ LT+R+EKYL
Sbjct: 481 EGAARRI----FTKPEVLLNICELLCFCIMQDASRTKCSFLQNNVTEKVLHLTRRKEKYL 540

Query: 541 VVAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKE 600
           VVAA+RF+RT+LS HD+++ NY VKNN+LKPI+D F+ANG+RYNLLNSAVL+LLE+IRK 
Sbjct: 541 VVAAIRFVRTLLSVHDDYVQNYVVKNNLLKPIIDVFIANGTRYNLLNSAVLDLLEHIRKG 600

Query: 601 NLKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRKRNDERAL 660
           N  LL++YI+D+FW+QL+ F+  +SIQA KVKYEQCLE+   K T + +DPR+R DERAL
Sbjct: 601 NATLLLKYIVDTFWDQLAPFQCLTSIQAFKVKYEQCLESAGPKSTSDAVDPRRRVDERAL 660

Query: 661 EKEEEDYFNEDSDEDDTASASVAPKQKVPS-------QPALSNGVTISYPPASPRSGGLV 720
           EKEEEDYFNEDSDE+D+ASAS   K+K  S       +P LSNGV  S   +SPRSGGLV
Sbjct: 661 EKEEEDYFNEDSDEEDSASASNTQKEKPASNIQKEQPKPHLSNGVAASPTSSSPRSGGLV 720

Query: 721 DYDDDDDDEDYKPPQRKQPDTS-DEDNEMMEALRKKRKLAPQEEPEMAKKQQLVKKHKPK 780
           DY+DD+DDEDYKPP RKQP+ S DE+ E++   RK   +  ++EP  +KK +L K  K +
Sbjct: 721 DYEDDEDDEDYKPPPRKQPEASEDEEGELLRLKRKSALVEREQEP--SKKPRLGKSSK-R 780

Query: 781 DGVFASLCSTLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSSDSSNCN 840
           + VFA LCSTLS AVL GKK+   +  S   S   K + +         S SS ++++ +
Sbjct: 781 ENVFAVLCSTLSHAVLTGKKSPGPA-GSAARSIVAKGAED---------SKSSEENNSSS 840

Query: 841 SDEENHREKESAASRSYSSSSVLHITSENRQLGGDDYPLIPPKSSPEMTVNGS 885
           SD+ENH++   ++S         H TS+N +L G++  ++ PKSSPEM VNGS
Sbjct: 841 SDDENHKDDGVSSSE--------HETSDNGKLNGEESLVVAPKSSPEMAVNGS 865

BLAST of MC05g0669 vs. TAIR 10
Match: AT3G06670.2 (binding )

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 586/914 (64.11%), Postives = 717/914 (78.45%), Query Frame = 0

Query: 1   MGALERSQTNSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETL 60
           MGA E+SQ+N+NSMQRVKVY LN+DGKWDD+GTGHV++D++ERSEEL L VIDEEDNETL
Sbjct: 1   MGAPEKSQSNTNSMQRVKVYHLNEDGKWDDRGTGHVSIDFVERSEELSLCVIDEEDNETL 60

Query: 61  LLHRISSDDIYQKQEDTIISWKDPEYSTELALSFQEATGCSFIWDNICSVQRNLHFSTLS 120
           L+H I+ +DIY+KQEDTIISW+DPE STELALSFQE  GCS++WD IC++QRNLHFS+L+
Sbjct: 61  LVHPINPEDIYRKQEDTIISWRDPERSTELALSFQETAGCSYVWDQICTMQRNLHFSSLN 120

Query: 121 NETFHSVNSELRELPAVDLTTLPLIHKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRI 180
           +ETFHS+NSELRELPAV+LTTLPLI K V ESGI DQMRLTEL+L D DFFR LM +F+I
Sbjct: 121 SETFHSLNSELRELPAVELTTLPLILKIVTESGITDQMRLTELILKDHDFFRNLMGVFKI 180

Query: 181 CEDLDDTDGLHMIFKIVRGIVLLNSPQIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNF 240
           CEDL++ DGLHMIF IV+GI+LLNS QI EKIF DE+IM+IIG LEYDP VPH+QH RNF
Sbjct: 181 CEDLENVDGLHMIFNIVKGIILLNSSQILEKIFGDELIMEIIGCLEYDPGVPHSQHHRNF 240

Query: 241 LKEHVVFKE---------------------AIPIKNPLVLSKIHQTYKVGYLKDVVLTRI 300
           LKEHVVFKE                     AIPIK+PLVLSKIHQTY++GYLKDVVL R+
Sbjct: 241 LKEHVVFKERQSHVFFVRKEHAHYGCFGISAIPIKDPLVLSKIHQTYRIGYLKDVVLARV 300

Query: 301 LDDSMVANLNSIIHANNAIVVSLLKDDNTFIQELFARLRSPATFDDSKKDLVYFLHEFCS 360
           LDD++VANLNS+IHANNAIVVSLLKDD+TFIQELFARLRSP+T  +SKK+LVYFLHEFCS
Sbjct: 301 LDDAIVANLNSVIHANNAIVVSLLKDDSTFIQELFARLRSPSTSMESKKNLVYFLHEFCS 360

Query: 361 LSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSGDEKLVLTGTDILILFLNQDPNLLRTHV 420
           LSKSLQ+VQQLRLFRDL+NEGIF +I +VLQ  D+KLVLTGTDILILFL QDPNLLR++V
Sbjct: 361 LSKSLQVVQQLRLFRDLINEGIFHVIEEVLQIPDKKLVLTGTDILILFLTQDPNLLRSYV 420

Query: 421 VRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLLDTYALS-GVQRDTIIEIFYEKHLG 480
           VR EG PL GLLVKGM+ DFGD MHCQFLEI+R LLD  ALS G QR  I++IFYEKHL 
Sbjct: 421 VRTEGNPLLGLLVKGMMEDFGDKMHCQFLEIIRTLLDANALSGGAQRANIMDIFYEKHLP 480

Query: 481 QLIDIITASCPTEGFQSTKKIEGAGVILGDTNSVKPEILSNICELLCFCVLQHPYRIKSN 540
           +L+D+ITASCP    +S+   EGA   +      KPE+L NICELLCFC++Q   R K +
Sbjct: 481 ELVDVITASCPE---KSSNASEGAARRI----FTKPEVLLNICELLCFCIMQDASRTKCS 540

Query: 541 FLLNNTIDKVVLLTQRREKYLVVAAVRFIRTILSRHDEHLINYFVKNNVLKPIVDAFVAN 600
           FL NN  +KV+ LT+R+EKYLVVAA+RF+RT+LS HD+++ NY VKNN+LKPI+D F+AN
Sbjct: 541 FLQNNVTEKVLHLTRRKEKYLVVAAIRFVRTLLSVHDDYVQNYVVKNNLLKPIIDVFIAN 600

Query: 601 GSRYNLLNSAVLELLEYIRKENLKLLVRYIIDSFWNQLSQFENWSSIQALKVKYEQCLEN 660
           G+RYNLLNSAVL+LLE+IRK N  LL++YI+D+FW+QL+ F+  +SIQA KVKYEQCLE+
Sbjct: 601 GTRYNLLNSAVLDLLEHIRKGNATLLLKYIVDTFWDQLAPFQCLTSIQAFKVKYEQCLES 660

Query: 661 CETKGTPNTLDPRKRNDERALEKEEEDYFNEDSDEDDTASASVAPKQKVPS-------QP 720
              K T + +DPR+R DERALEKEEEDYFNEDSDE+D+ASAS   K+K  S       +P
Sbjct: 661 AGPKSTSDAVDPRRRVDERALEKEEEDYFNEDSDEEDSASASNTQKEKPASNIQKEQPKP 720

Query: 721 ALSNGVTISYPPASPRSGGLVDYDDDDDDEDYKPPQRKQPDTS-DEDNEMMEALRKKRKL 780
            LSNGV  S   +SPRSGGLVDY+DD+DDEDYKPP RKQP+ S DE+ E++   RK   +
Sbjct: 721 HLSNGVAASPTSSSPRSGGLVDYEDDEDDEDYKPPPRKQPEASEDEEGELLRLKRKSALV 780

Query: 781 APQEEPEMAKKQQLVKKHKPKDGVFASLCSTLSQAVLPGKKTMNASHTSPCTSYENKSSA 840
             ++EP  +KK +L K  K ++ VFA LCSTLS AVL GKK+   +  S   S   K + 
Sbjct: 781 EREQEP--SKKPRLGKSSK-RENVFAVLCSTLSHAVLTGKKSPGPA-GSAARSIVAKGAE 840

Query: 841 EENHKTDPAISSSSSDSSNCNSDEENHREKESAASRSYSSSSVLHITSENRQLGGDDYPL 885
           +         S SS ++++ +SD+ENH++   ++S         H TS+N +L G++  +
Sbjct: 841 D---------SKSSEENNSSSSDDENHKDDGVSSSE--------HETSDNGKLNGEESLV 886

BLAST of MC05g0669 vs. TAIR 10
Match: AT5G49390.1 (LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 11 growth stages; BEST Arabidopsis thaliana protein match is: binding (TAIR:AT3G06670.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 208.4 bits (529), Expect = 2.4e-53
Identity = 185/496 (37.30%), Postives = 258/496 (52.02%), Query Frame = 0

Query: 371 SGDEKLVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEI 430
           S D+KLVL G  IL + L QD   L ++VVR E + L GLLVKGM+ DFGD M   F++I
Sbjct: 2   SSDKKLVLMGAKILSVLLAQDSIRLCSYVVRPETY-LLGLLVKGMMEDFGDEMESLFVDI 61

Query: 431 LRNLLDTYALSGVQRDT-----IIEIFYEKHLGQLIDIITASCPTEGFQSTKKIEGAGVI 490
           ++N+L         + T     I +IF EKHL +L+D I ASCP     ++   EGA V 
Sbjct: 62  IQNVLGCGGAQVSDKKTNMQRIIWDIFCEKHLPELVDFIMASCPERPGDTS---EGAFVR 121

Query: 491 LGDTNSVKPEILSNICELLCFCVLQHPYRIKSNFLLNNTIDKVVLLTQRREKYLVVAAVR 550
           +G     KP IL  ICELLC CV   P R  +NFL N+ I+KV+LLT+R+EK LV AAVR
Sbjct: 122 VGSHRGTKPGILLRICELLCSCVQLDPSR--TNFLHNSVIEKVLLLTRRKEKTLVAAAVR 181

Query: 551 FIRTILSRHDEHLINYFVKNNVLKPIVDAFVANGSRYNLLNSAVLELLEYIRKENLKLLV 610
           F+RT+LS H++++ +Y V+NN LKPI++ FVAN  R NL+ +AVLELLE+IRKE   +LV
Sbjct: 182 FVRTLLSVHNDNVQSYIVENNTLKPIIEVFVANRHRDNLMTAAVLELLEHIRKE-YAVLV 241

Query: 611 RYIIDSFWNQLSQFENWSSIQALKVKYEQCLENCETKGTPNTLDPRK--RNDERAL-EKE 670
           +Y+ D+FW+QL+ FEN  SI+ LK KYE        KG  +T D     R D RAL EK+
Sbjct: 242 KYVGDTFWDQLAPFENLRSIKDLKTKYE--------KGPKSTTDDESDMRQDGRALDEKK 301

Query: 671 EEDYFNEDSDEDD------TASASVAPKQKVPSQP------ALSNGVTISYPPASPRSGG 730
                +   +E D      TA++S +  Q   ++P      A S+  +     A P + G
Sbjct: 302 PASASSTQMEEADPHNSNATAASSASSTQMEDAEPYNPDVTAASSTCSTQMEVAEPYNPG 361

Query: 731 LVDYDDD-----DDDEDYKPPQRKQPDTSDEDNEMMEALRKKRKLAPQEEPEMAKKQQLV 790
           +           ++ + Y P       TS    E+ E        A           ++ 
Sbjct: 362 VTAASSTSSTQMEEADPYNPDVTAASSTSITPMEVAEPYNPDVTAASSTS---ITPMEVA 421

Query: 791 KKHKPKDGVFASLCSTLSQAVLPGKKTMNASHTSPCTSYENKSSAEENHKTDPAISSSSS 842
           + + P  GV      T + +V P K+      +     YE+     E  K       SS+
Sbjct: 422 EPYNP--GV------TAASSVSPSKR------SGGLVDYEDDEDELEKSKRQ---KLSST 462

BLAST of MC05g0669 vs. TAIR 10
Match: AT5G49380.1 (CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF625 (InterPro:IPR006887); BEST Arabidopsis thaliana protein match is: binding (TAIR:AT3G06670.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 202.2 bits (513), Expect = 1.7e-51
Identity = 106/190 (55.79%), Postives = 143/190 (75.26%), Query Frame = 0

Query: 147 KTVVESGIADQMRLTELLLNDQDFFRKLMNLFRICEDLDDTDGLHMIFKIVRGIVLLNSP 206
           + V + G  DQMRLTEL+L +  FF+KL+++F  CE+  D DGLHM+F IV+ I+ +N+ 
Sbjct: 9   QVVADYGNTDQMRLTELMLKNGAFFQKLIDVFDDCENRKDIDGLHMMFNIVKEIISVNNY 68

Query: 207 QIFEKIFSDEMIMDIIGSLEYDPEVPHTQHFRNFLKEHVVFKEAIPIKNPLVLSKIHQTY 266
           QI E I  D++ M I G LEYDP+VP ++  R  L+++VVF E IPIKNPLVLSKIHQTY
Sbjct: 69  QILEIILGDQLFMKIFGCLEYDPDVPQSKDHRTSLRKNVVFVEDIPIKNPLVLSKIHQTY 128

Query: 267 KVGYLKDVVLTRILDDSMVANLNSIIHANNAIVVSLLKDDNTFIQELFARLRSPATFDDS 326
           ++ +LKDVVLT +LD +  A L+S+I+AN A V++LLKDD   IQE FARLRSP+T D+S
Sbjct: 129 RIDFLKDVVLTDVLDVATSAFLDSVINANKATVLTLLKDD---IQESFARLRSPSTSDES 188

Query: 327 KKDLVYFLHE 337
           + +LV+ LHE
Sbjct: 189 RNNLVFPLHE 195

BLAST of MC05g0669 vs. TAIR 10
Match: AT5G49370.1 (Pleckstrin homology (PH) domain superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 4.5e-36
Identity = 66/95 (69.47%), Postives = 84/95 (88.42%), Query Frame = 0

Query: 10  NSNSMQRVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDD 69
           N+NSMQRVKVYRLN+DG+WDD+GTGH+T+DYMERSE   L+VIDE+DN TLL HRIS D+
Sbjct: 6   NTNSMQRVKVYRLNEDGQWDDKGTGHITMDYMERSEVFNLYVIDEDDNATLLAHRISIDN 65

Query: 70  IYQKQEDTIISWKDPEYSTELALSFQEATGCSFIW 105
           IY++Q+D+IISW DP++S +LALSFQE  GC+ +W
Sbjct: 66  IYKQQDDSIISWIDPQHSAQLALSFQETAGCTIVW 100