MC05g0552 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0552 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 9.7e-162
Identity = 292/427 (68.38%), Postives = 344/427 (80.56%), Query Frame = 0

Query: 10  FFFFTSISATTSFRPKSLVLPVIKDP-SLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCN 69
           F   T+  + TSFRPK L+LP+ KD  +LQY+TQ++QRTP VP+ LT+DLGGQFLWVDC+
Sbjct: 17  FITSTTAQSETSFRPKGLILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCD 76

Query: 70  HSYVSSTYKPARCRSAQCSLANSRT-CGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEV 129
             YVSSTY+PARCRSAQCSLA + + CG+CFSPPKPGCNN+TC  LPDNT+    T GE+
Sbjct: 77  QGYVSSTYRPARCRSAQCSLAGAGSGCGQCFSPPKPGCNNNTCGLLPDNTITRTATSGEL 136

Query: 130 ASDVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSA 189
           ASD V +QSS+G NPGR VS  +FLFVCG+TFLLEGLA GV GMAG GRTRISLP QFSA
Sbjct: 137 ASDTVQVQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSA 196

Query: 190 AFSFNRKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIST--LIYTPLLINPVSTAGAFF 249
            FSF RKFAVCLSSST + G + F G+G Y  LPN E +     YTPL INPVSTA AF 
Sbjct: 197 EFSFPRKFAVCLSSSTNSKGVVLF-GDGPYTFLPNREFANNDFSYTPLFINPVSTASAFS 256

Query: 250 DGDRSAEYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVK 309
             + S+EYFIGVKSI I EK VP+N +LL ID+ G+GGTKISTVNPYT+LE+SIYNAV  
Sbjct: 257 SREPSSEYFIGVKSIKINEKVVPINTTLLSIDNQGVGGTKISTVNPYTILETSIYNAVTN 316

Query: 310 IFVEQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANS 369
            FV++L       RVA+VAPF ACFD +NI+STRVGPAVP+IDLVLQ++ V WRIFGANS
Sbjct: 317 FFVKEL---VNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANS 376

Query: 370 MVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCAN 429
           MV+V+  VLCLGFVDGGV+PRT+IV+G + IEDNLL+FDLA SRLGF+S++LFRQTTCAN
Sbjct: 377 MVQVSENVLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCAN 436

Query: 430 FNFTSNA 433
           FNFTS A
Sbjct: 437 FNFTSIA 439

BLAST of MC05g0552 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 8.6e-94
Identity = 202/443 (45.60%), Postives = 272/443 (61.40%), Query Frame = 0

Query: 1   SFTFFFIFFFFFFTSISATTS-----FRPKSLVLPVIKDPSLQ-YITQLRQRTPFVPLKL 60
           +F  FF        + SAT S      RP  LVL V KD +   ++ Q+ +RTP V    
Sbjct: 7   NFLLFFCSLSLIILTSSATKSQTHVPIRPNKLVLKVQKDRATNLHVVQIHKRTPLVQFPF 66

Query: 61  TVDLGGQFLWVDCNHSYVSSTYKPARCRSAQCSLANSRTCGEC-FSPPKPGCNNDTCSHL 120
            +DL G+FL V+C + Y SSTYK   C S+QC+ ANS TC  C  S  +PGC+ + C  L
Sbjct: 67  VIDLTGRFLSVNCENQYTSSTYKAPVCHSSQCARANSHTCRTCSSSKTRPGCHTNACGLL 126

Query: 121 PDNTVVGLGTIGEVASDVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLE-GLAGGVHGMA 180
             N V      GE+A DV+ + S+ GS+PG +V+ P+FLF C  + +L+ GL   V G+A
Sbjct: 127 TTNPVTQQSAQGELAEDVLKIPSTQGSSPGPMVTYPHFLFACAPSNILQKGLPKNVQGVA 186

Query: 181 GFGRTRISLPPQFSAAFSFNRKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYT 240
           G G + ISLP Q ++ F F  KFAVCL+SS    GA+FF G G YF+ P I++S  L Y 
Sbjct: 187 GLGHSPISLPYQLASHFGFPPKFAVCLTSSPGKNGAVFF-GEGPYFMKPGIDVSRQLTYA 246

Query: 241 PLLINPVSTAGAFFDGDRSAEYFIGVKSILIAEKNVPLNNSLL-KIDSHGIGGTKISTVN 300
           P  I             +  EY+I V+S  I       NN++L  I   G GG  IST  
Sbjct: 247 PFTIG------------QQGEYYINVQSFKI-------NNAMLPSIPKGGFGGAMISTTT 306

Query: 301 PYTVLESSIYNAVVKIFVEQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLV 360
           PYT L++ I+ A+ ++F+ QLR       V  VAPFGACFD   I ++++GP VP+IDLV
Sbjct: 307 PYTTLQTPIFRALNQLFMNQLR---GVPHVKPVAPFGACFDANRIPTSKMGPTVPSIDLV 366

Query: 361 LQSKK-VIWRIFGANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSR 420
           L +KK ++WRIFGAN+M++   GV+CL FVDGG+ P+  IVIG  Q+EDNLL+FDL +SR
Sbjct: 367 LDNKKNIMWRIFGANAMIQPRPGVMCLAFVDGGMRPKAPIVIGTQQLEDNLLQFDLMNSR 426

Query: 421 LGFSSTLLFRQTTCANFNFTSNA 433
           LGFSS+LLFR+T CANFNF +++
Sbjct: 427 LGFSSSLLFRRTNCANFNFGTSS 426

BLAST of MC05g0552 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 292.7 bits (748), Expect = 6.6e-78
Identity = 170/410 (41.46%), Postives = 230/410 (56.10%), Query Frame = 0

Query: 27  LVLPVIKDPSLQ-YITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVSSTYKPARCRSAQ 86
           L+ P+ KD + Q Y   +  +TP  P KL + LG    WV C+ +Y SS+     C +  
Sbjct: 28  LIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLCDSTYTSSSSHHIPCNTPL 87

Query: 87  CSLANSRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVVSLQSSDGSNPGRV 146
           C+   S  C           N+  C+  P+N V     +     D ++L + D S+   +
Sbjct: 88  CNSFPSNACSN---------NSSLCALFPENPVTRNTLLDTALIDSLALPTYDASS--SL 147

Query: 147 VSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFNRKFAVCLSSSTAA 206
           V I +F+F C T  LL+GLA    G+A  GR+  SLP Q S + +  R F +CL +S+A 
Sbjct: 148 VLISDFIFSCATAHLLQGLAANALGLASLGRSNYSLPAQISTSLTSPRSFTLCLPASSAN 207

Query: 207 PGAIFFGGNGSYFLLPNIEISTLIYTPLLINPVSTAGAFFDGDRSAEYFIGVKSILIAEK 266
            GA  F    S FL  +     L YT L++NPV+      +   S EYFI + SI I  K
Sbjct: 208 TGAAIFASTASSFLFSS--KIDLTYTQLIVNPVADTVVTDNPQPSDEYFINLTSIKINGK 267

Query: 267 NVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQLRLQGEEIRVAAVAP 326
            + +N+S+L +D  G GGTKIST  PYTVLE+SIY   V+ FV +       +   AV P
Sbjct: 268 PLYINSSILTVDQTGFGGTKISTAEPYTVLETSIYRLFVQRFVNESSAFNLTV-TEAVEP 327

Query: 327 FGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNGG---VLCLGFVDGG 386
           FG C+   +++ TRVGPAVPT+DLV+ S+ V WRIFG NSMV V  G   V CLGFVDGG
Sbjct: 328 FGVCYPAGDLTETRVGPAVPTVDLVMHSEDVFWRIFGGNSMVRVAKGGVDVWCLGFVDGG 387

Query: 387 VNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTSNA 433
              RT IVIG HQ+EDNL++FDL S+R GF+STLL +   C+N    + A
Sbjct: 388 TRGRTPIVIGGHQLEDNLMQFDLDSNRFGFTSTLLLQDAKCSNLKVNNFA 423

BLAST of MC05g0552 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 9.6e-77
Identity = 171/426 (40.14%), Postives = 238/426 (55.87%), Query Frame = 0

Query: 19  TTSFRPKSLVLPVIKDPSLQ-YITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVSSTYK 78
           T+S +P  LVLP+ +D S + +   + +RTP + + + +DL G+ LWV C+  Y SSTY+
Sbjct: 40  TSSSKPNLLVLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTYQ 99

Query: 79  PARCRSAQCSLANSRTCGEC--FSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVVSLQ 138
              C S QCS AN+  C  C   +  +PGC+N+TC  +  N V     +GE+A DV++L 
Sbjct: 100 APFCHSTQCSRANTHQCFTCTDSTTSRPGCHNNTCGLISSNPVTQESGLGELAQDVLALH 159

Query: 139 SSDGSNPGRVVSIPNFLFVCGTTFLLE-GLAGGVHGMAGFGRTRISLPPQFSAAFSFNRK 198
           S+ GS  G +V IP FLF C  TFL + GL   V G  G G   ISLP Q  + F   R+
Sbjct: 160 STHGSKLGSLVKIPQFLFSCAPTFLTQKGLPNNVQGALGLGHAPISLPNQLFSHFGLKRQ 219

Query: 199 FAVCLSSSTAAPGAIFFG-----GNGSYFLLPNIEISTLIYTPLLINPVSTAGAFFDGDR 258
           F +CLSS   + GAI FG      N +Y       +  ++YTPL I+            +
Sbjct: 220 FTMCLSSYPTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTPLTIS------------K 279

Query: 259 SAEYFIGV------KSILIAEKN---VPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIY 318
             EYFI V      K ++I  KN    P ++S    +S  IGG  I+T NPYTVL  SI+
Sbjct: 280 QGEYFIQVSAIRVNKHMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLRHSIF 339

Query: 319 NAVVKIFVEQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRI 378
               ++F   +  Q +   V AV PFG C+D K IS       VP++DL++    V+WRI
Sbjct: 340 EVFTQVFANNVPKQAQ---VKAVGPFGLCYDTKKISG-----GVPSVDLIMDKSDVVWRI 399

Query: 379 FGANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGF-SSTLLFR 426
            G N MV+   GV CLGFVDGGV+ R  I +G HQ+E+NL+ FDLA SR+GF +++L   
Sbjct: 400 SGENLMVQAQDGVSCLGFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTNSLKSH 445

BLAST of MC05g0552 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 3.4e-74
Identity = 169/426 (39.67%), Postives = 233/426 (54.69%), Query Frame = 0

Query: 17  SATTSFRPKSLVLPVIKDPSL-QYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVSST 76
           S  TS +P  LVLPV +D S   +   + +RTP + + L +DL G+ LWV C+  Y SST
Sbjct: 37  SQPTSSKPNLLVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSST 96

Query: 77  YKPARCRSAQCSLANSRTCGEC--FSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVVS 136
           Y+   C S QCS AN+  C  C   +  +PGC+N+TC  L  N V     +GE+A DV++
Sbjct: 97  YQAPFCHSTQCSRANTHQCFTCTDSTTTRPGCHNNTCGLLSSNPVTQESGLGELAQDVLA 156

Query: 137 LQSSDGSNPGRVVSIPNFLFVCGTTFLLE-GLAGGVHGMAGFGRTRISLPPQFSAAFSFN 196
           + S+ GS  G +V +P FLF C  +FL + GL   V G  G G+  ISL  Q  + F   
Sbjct: 157 IHSTHGSKLGPMVKVPQFLFSCAPSFLAQKGLPNNVQGALGLGQAPISLQNQLFSHFGLK 216

Query: 197 RKFAVCLSSSTAAPGAIFFG-----GNGSYFLLPNIEISTLIYTPLLINPVSTAGAFFDG 256
           R+F+VCLS  + + GAI FG      N +Y       +  L+YTPL I+           
Sbjct: 217 RQFSVCLSRYSTSNGAILFGDINDPNNNNYIHNSLDVLHDLVYTPLTIS----------- 276

Query: 257 DRSAEYFIGVKSILIAEKNV-PLNNSLLKIDS---HG---IGGTKISTVNPYTVLESSIY 316
            +  EYFI V +I + +  V P  N  +   S   HG   IGG  I+T +PYTVL  SI+
Sbjct: 277 -KQGEYFIQVNAIRVNKHLVIPTKNPFISPSSTSYHGSGEIGGALITTTHPYTVLSHSIF 336

Query: 317 NAVVKIFVEQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRI 376
               ++F   +  Q +   V AV PFG C+D + IS        P++DL+L     +WRI
Sbjct: 337 EVFTQVFANNMPKQAQ---VKAVGPFGLCYDSRKISG-----GAPSVDLILDKNDAVWRI 396

Query: 377 FGANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGF-SSTLLFR 426
              N MV+   GV CLGFVDGGV+ R  I +GAH +E+NL+ FDL  SR+GF S++L   
Sbjct: 397 SSENFMVQAQDGVSCLGFVDGGVHARAGIALGAHHLEENLVVFDLERSRVGFNSNSLKSY 442

BLAST of MC05g0552 vs. NCBI nr
Match: XP_038896289.1 (probable aspartic proteinase GIP2 [Benincasa hispida])

HSP 1 Score: 628 bits (1620), Expect = 4.54e-223
Identity = 319/417 (76.50%), Postives = 354/417 (84.89%), Query Frame = 0

Query: 18  ATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVSSTYK 77
           A TSFRPKSLVLPV K PSLQYITQ+ QRTP VP+KLTVDLGGQF+WVDC+  YVSSTYK
Sbjct: 23  AKTSFRPKSLVLPVTKHPSLQYITQIHQRTPLVPVKLTVDLGGQFMWVDCDRGYVSSTYK 82

Query: 78  PARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVVSLQS 137
           PARCRSAQCSLA+ S +CG+CFSPP+PGCNN+TC   P NT++ L T GEVASDVVS+ S
Sbjct: 83  PARCRSAQCSLASKSSSCGQCFSPPRPGCNNNTCGLFPGNTIIRLSTSGEVASDVVSVSS 142

Query: 138 SDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFNRKFA 197
           +DG NP R VS+PNFLFVCG+TFLLEGLAGGV GMAGFGRT ISLP QF+AAFSFNRKFA
Sbjct: 143 TDGFNPTRAVSLPNFLFVCGSTFLLEGLAGGVTGMAGFGRTGISLPSQFAAAFSFNRKFA 202

Query: 198 VCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSAEYFI 257
           VCLS ST  PG IF  GNG Y  LPN +++ +L YTPL INPVSTAG F  G++SAEYFI
Sbjct: 203 VCLSGSTRFPGVIF-SGNGPYHFLPNTDLTKSLTYTPLFINPVSTAGVFSAGEKSAEYFI 262

Query: 258 GVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQLRLQG 317
           GVKSI+I  K VPLN +LLKIDS+G GGTKISTVNPYTVLESSIYNAVVK F  +L    
Sbjct: 263 GVKSIVINSKTVPLNTTLLKIDSNGNGGTKISTVNPYTVLESSIYNAVVKTFTTEL---S 322

Query: 318 EEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNGGVLC 377
           +  RVAAVAPFG C++ K+ SSTR+GP VP+IDL+LQ+KKVIWRIFGANSMV VN  VLC
Sbjct: 323 KVPRVAAVAPFGVCYNSKSFSSTRLGPGVPSIDLILQNKKVIWRIFGANSMVFVNDNVLC 382

Query: 378 LGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTSNA 432
           LGFVDGGV PRTAIVIGAHQIEDNLLEFDLA+SRLGFSSTLL R TTCANFNFTSNA
Sbjct: 383 LGFVDGGVEPRTAIVIGAHQIEDNLLEFDLATSRLGFSSTLLGRMTTCANFNFTSNA 435

BLAST of MC05g0552 vs. NCBI nr
Match: XP_023538677.1 (basic 7S globulin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620 bits (1600), Expect = 4.83e-220
Identity = 310/415 (74.70%), Postives = 356/415 (85.78%), Query Frame = 0

Query: 18  ATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVSSTYK 77
           ATTSFRPK+LVLPV K PSLQYITQ+RQRTP VP+KLTVDLG QF+WVDC+  Y+SSTYK
Sbjct: 22  ATTSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLTVDLGSQFMWVDCDRGYISSTYK 81

Query: 78  PARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVVSLQS 137
           PARCRSAQC+LA+ S  CG+CFSPP+PGCNN+TCS  P NT++ L T GE+ASDVVS+ S
Sbjct: 82  PARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFPGNTIIHLSTSGELASDVVSVSS 141

Query: 138 SDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFNRKFA 197
           +DG NP + V++PNFLFVCG+TFLL+GLAGGV GMAGFGR  ISLP QFSAAFSFNRKFA
Sbjct: 142 TDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGFGRNGISLPSQFSAAFSFNRKFA 201

Query: 198 VCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSAEYFI 257
           VCLS ST  PG IF  GNG Y  LPNI+++ +L YTPL INPVSTAG F  G++S EYFI
Sbjct: 202 VCLSGSTRFPGVIF-SGNGPYHFLPNIDLTDSLTYTPLFINPVSTAGVFSAGEKSTEYFI 261

Query: 258 GVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQLRLQG 317
           GVKSILI  K VPLN +LLKIDS+G+GGTKISTV+PYTVLESSIYNAV+K F  +L+   
Sbjct: 262 GVKSILINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTVLESSIYNAVLKTFTTELK--- 321

Query: 318 EEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNGGVLC 377
              RVAAVAPFGACF+ K+ISSTR+GP VP+I+L+LQ+KKVIWRIFGANSMV+V   VLC
Sbjct: 322 NVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVIWRIFGANSMVQVKDDVLC 381

Query: 378 LGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTS 430
           LGFVDGGVNPRT+IVIGAHQIEDNLLEFD+A+SRLGFS+TLL R TTCANFNFTS
Sbjct: 382 LGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLLGRMTTCANFNFTS 432

BLAST of MC05g0552 vs. NCBI nr
Match: KAG6597463.1 (putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619 bits (1597), Expect = 1.43e-219
Identity = 311/419 (74.22%), Postives = 357/419 (85.20%), Query Frame = 0

Query: 14  TSISATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVS 73
           +S  A TSFRPK+LVLPV K PSLQYITQ+RQRTP VP+KLTVDLG QF+WVDC+  Y+S
Sbjct: 19  SSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLTVDLGSQFMWVDCDRGYIS 78

Query: 74  STYKPARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVV 133
           STYKPARCRSAQC+LA+ S  CG+CFSPP+PGCNN+TCS  P NT++ L T GE+ASDVV
Sbjct: 79  STYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFPGNTIIHLSTSGELASDVV 138

Query: 134 SLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFN 193
           S+ S+DG NP + V++PNFLFVCG+TFLL+GLAGGV GMAGFGR  ISLP QFSAAFSFN
Sbjct: 139 SVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGFGRNGISLPSQFSAAFSFN 198

Query: 194 RKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSA 253
           RKFAVCLS ST  PG IF  GNG Y  LPNI+++ +L YTPL INPVSTAG F  G++S 
Sbjct: 199 RKFAVCLSGSTRFPGVIF-AGNGPYHFLPNIDLTDSLTYTPLFINPVSTAGVFSAGEKST 258

Query: 254 EYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQL 313
           EYFIGVKSILI  K VPLN +LLKIDS+GIGGTKISTV+PYTVLESSIYNAV+K F  +L
Sbjct: 259 EYFIGVKSILINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTVLESSIYNAVLKTFTTEL 318

Query: 314 RLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNG 373
           +      RVAAVAPFGACF+ K+ISSTR+GP VP+I+L+LQ+KKVIWRIFGANSMV+V  
Sbjct: 319 K---NVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVIWRIFGANSMVQVKD 378

Query: 374 GVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTS 430
            VLCLGFVDGGVNPRT+IVIGAHQIEDNLLEFD+A+SRLGFS+TLL R TTCANFNFTS
Sbjct: 379 DVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLLGRMTTCANFNFTS 433

BLAST of MC05g0552 vs. NCBI nr
Match: KAG7028922.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 619 bits (1596), Expect = 2.03e-219
Identity = 311/419 (74.22%), Postives = 357/419 (85.20%), Query Frame = 0

Query: 14  TSISATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVS 73
           +S  A TSFRPK+LVLPV K PSLQYITQ+RQRTP VP+KLTVDLG QF+WVDC+  Y+S
Sbjct: 19  SSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLTVDLGSQFMWVDCDRGYIS 78

Query: 74  STYKPARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVV 133
           STYKPARCRSAQC+LA+ S  CG+CFSPP+PGCNN+TCS  P NT++ L T GE+ASDVV
Sbjct: 79  STYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFPGNTIIHLSTSGELASDVV 138

Query: 134 SLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFN 193
           S+ S+DG NP + V++PNFLFVCG+TFLL+GLAGGV GMAGFGR  ISLP QFSAAFSFN
Sbjct: 139 SVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGFGRNGISLPSQFSAAFSFN 198

Query: 194 RKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSA 253
           RKFAVCLS ST  PG IF  GNG Y  LPNI+++ +L YTPL INPVSTAG F  G++S 
Sbjct: 199 RKFAVCLSGSTRFPGVIF-AGNGPYHFLPNIDLTDSLTYTPLFINPVSTAGVFSAGEKST 258

Query: 254 EYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQL 313
           EYFIGVKSI+I  K VPLN +LLKIDS+GIGGTKISTV+PYTVLESSIYNAV+K F  +L
Sbjct: 259 EYFIGVKSIVINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTVLESSIYNAVLKTFTTEL 318

Query: 314 RLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNG 373
           +      RVAAVAPFGACF+ K+ISSTR+GP VP+I+L+LQ+KKVIWRIFGANSMV+V  
Sbjct: 319 K---NVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVIWRIFGANSMVQVKD 378

Query: 374 GVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTS 430
            VLCLGFVDGGVNPRT+IVIGAHQIEDNLLEFDLA+SRLGFS+TLL R TTCANFNFTS
Sbjct: 379 DVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDLATSRLGFSATLLGRMTTCANFNFTS 433

BLAST of MC05g0552 vs. NCBI nr
Match: XP_022936844.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 618 bits (1594), Expect = 4.09e-219
Identity = 310/419 (73.99%), Postives = 357/419 (85.20%), Query Frame = 0

Query: 14  TSISATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVS 73
           +S  A TSFRPK+LVLPV K PSLQYITQ+RQRTP VP+KLTVDLG QF+WVDC+  Y+S
Sbjct: 19  SSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLTVDLGSQFMWVDCDRGYIS 78

Query: 74  STYKPARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVV 133
           STYKPARCRSAQC+LA+ S  CG+CFSPP+PGCNN+TCS  P NT++ L T GE+ASDVV
Sbjct: 79  STYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFPGNTIIHLSTSGELASDVV 138

Query: 134 SLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFN 193
           S+ S+DG NP + V++PNFLFVCG+TFLL+GLAGGV GMAGFGR  ISLP QFSAAFSFN
Sbjct: 139 SVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGFGRNGISLPSQFSAAFSFN 198

Query: 194 RKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSA 253
           RKFAVCLS ST  PG IF  GNG Y  LPNI+++ +L YTPL INPVSTAG F  G++S 
Sbjct: 199 RKFAVCLSGSTRFPGVIF-SGNGPYHFLPNIDLTDSLTYTPLFINPVSTAGVFTAGEKST 258

Query: 254 EYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQL 313
           EYFIGVKSI+I  K VPLN +LLKIDS+GIGGTKISTV+PYTVLESSIYNAV+K F  +L
Sbjct: 259 EYFIGVKSIVINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTVLESSIYNAVLKTFTTEL 318

Query: 314 RLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNG 373
           +      RVAAVAPFGACF+ K+ISSTR+GP VP+I+L+LQ+KKVIWRIFGANSMV+V  
Sbjct: 319 K---NVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVIWRIFGANSMVQVKD 378

Query: 374 GVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTS 430
            VLCLGFVDGGVNPRT+IVIGAHQIEDNLLEFD+A+SRLGFS+TLL R TTCANFNFTS
Sbjct: 379 DVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLLGRMTTCANFNFTS 433

BLAST of MC05g0552 vs. ExPASy TrEMBL
Match: A0A6J1F9F8 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111443306 PE=4 SV=1)

HSP 1 Score: 618 bits (1594), Expect = 1.98e-219
Identity = 310/419 (73.99%), Postives = 357/419 (85.20%), Query Frame = 0

Query: 14  TSISATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVS 73
           +S  A TSFRPK+LVLPV K PSLQYITQ+RQRTP VP+KLTVDLG QF+WVDC+  Y+S
Sbjct: 19  SSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLTVDLGSQFMWVDCDRGYIS 78

Query: 74  STYKPARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVV 133
           STYKPARCRSAQC+LA+ S  CG+CFSPP+PGCNN+TCS  P NT++ L T GE+ASDVV
Sbjct: 79  STYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFPGNTIIHLSTSGELASDVV 138

Query: 134 SLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFN 193
           S+ S+DG NP + V++PNFLFVCG+TFLL+GLAGGV GMAGFGR  ISLP QFSAAFSFN
Sbjct: 139 SVSSTDGFNPTKPVTVPNFLFVCGSTFLLDGLAGGVTGMAGFGRNGISLPSQFSAAFSFN 198

Query: 194 RKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSA 253
           RKFAVCLS ST  PG IF  GNG Y  LPNI+++ +L YTPL INPVSTAG F  G++S 
Sbjct: 199 RKFAVCLSGSTRFPGVIF-SGNGPYHFLPNIDLTDSLTYTPLFINPVSTAGVFTAGEKST 258

Query: 254 EYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQL 313
           EYFIGVKSI+I  K VPLN +LLKIDS+GIGGTKISTV+PYTVLESSIYNAV+K F  +L
Sbjct: 259 EYFIGVKSIVINSKTVPLNTTLLKIDSNGIGGTKISTVDPYTVLESSIYNAVLKTFTTEL 318

Query: 314 RLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNG 373
           +      RVAAVAPFGACF+ K+ISSTR+GP VP+I+L+LQ+KKVIWRIFGANSMV+V  
Sbjct: 319 K---NVPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVIWRIFGANSMVQVKD 378

Query: 374 GVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTS 430
            VLCLGFVDGGVNPRT+IVIGAHQIEDNLLEFD+A+SRLGFS+TLL R TTCANFNFTS
Sbjct: 379 DVLCLGFVDGGVNPRTSIVIGAHQIEDNLLEFDMATSRLGFSATLLGRMTTCANFNFTS 433

BLAST of MC05g0552 vs. ExPASy TrEMBL
Match: A0A6J1IGY1 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111475005 PE=4 SV=1)

HSP 1 Score: 618 bits (1593), Expect = 2.81e-219
Identity = 310/419 (73.99%), Postives = 356/419 (84.96%), Query Frame = 0

Query: 14  TSISATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVS 73
           +S  A TSFRPK+LVLPV K PSLQYITQ+RQRTP VPLKLTVDLG QF+WVDC+  Y+S
Sbjct: 19  SSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPLKLTVDLGSQFMWVDCDRGYIS 78

Query: 74  STYKPARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVV 133
           STYKPARCRSAQC+LA+ S  CG+CFSPP+PGCNN+TCS  P NT++ L T GE+ASDVV
Sbjct: 79  STYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFPGNTIIHLSTSGELASDVV 138

Query: 134 SLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFN 193
           S+ S+DG NP + V++PNFLFVCGTTFLL+GLAGGV GMAGFGR  ISLP QFSAAFSFN
Sbjct: 139 SVSSTDGFNPTKPVTVPNFLFVCGTTFLLDGLAGGVTGMAGFGRNGISLPSQFSAAFSFN 198

Query: 194 RKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSA 253
           RKFA+CLS ST+ PG IF  GNG Y  LPNI+++ +L YTPL INPVSTAG F  G++S 
Sbjct: 199 RKFAICLSGSTSFPGVIF-SGNGPYHFLPNIDLTDSLTYTPLFINPVSTAGVFTTGEKST 258

Query: 254 EYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQL 313
           EYFIGVKSI+I  K VPLN +LLKIDS+G+GGTKISTV+PYTVLESSIYNAV+K F   L
Sbjct: 259 EYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTVLESSIYNAVLKTFTTAL 318

Query: 314 RLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNG 373
           +      RVAAVAPFGACF+ K+ISSTR+GP VP+I+L+LQ+KKVIWRIFGANSMV V  
Sbjct: 319 K---NIPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVIWRIFGANSMVHVKD 378

Query: 374 GVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTS 430
            VLCLGFVDGGVNPRT+IVIGAHQIE+NLLEFDLA+SRLGFS+TLL R TTCANFNFTS
Sbjct: 379 DVLCLGFVDGGVNPRTSIVIGAHQIENNLLEFDLATSRLGFSATLLGRMTTCANFNFTS 433

BLAST of MC05g0552 vs. ExPASy TrEMBL
Match: A0A6J1IFL5 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111472330 PE=4 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 8.05e-219
Identity = 309/419 (73.75%), Postives = 356/419 (84.96%), Query Frame = 0

Query: 14  TSISATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVS 73
           +S  A TSFRPK+LVLPV K PSLQYITQ+RQRTP VP+KLTVDLG QF+WVDC+  Y+S
Sbjct: 19  SSAIAATSFRPKALVLPVTKHPSLQYITQIRQRTPLVPVKLTVDLGSQFMWVDCDRGYIS 78

Query: 74  STYKPARCRSAQCSLAN-SRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVV 133
           STYKPARCRSAQC+LA+ S  CG+CFSPP+PGCNN+TCS  P NT++ L T GE+ASDVV
Sbjct: 79  STYKPARCRSAQCNLASKSSGCGQCFSPPRPGCNNNTCSLFPGNTIIHLSTSGELASDVV 138

Query: 134 SLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFN 193
           S+ S+DG NP + V++PNFLFVCGTTFLL+GLAGGV GMAGFGR  ISLP QFSAAFSFN
Sbjct: 139 SVSSTDGFNPTKPVTVPNFLFVCGTTFLLDGLAGGVTGMAGFGRNGISLPSQFSAAFSFN 198

Query: 194 RKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSA 253
           RKFA+CLS ST+ PG IF  GNG Y  LPNI+++ +L YTPL INPVSTAG F  G++S 
Sbjct: 199 RKFAICLSGSTSFPGVIF-SGNGPYHFLPNIDLTDSLTYTPLFINPVSTAGVFTTGEKST 258

Query: 254 EYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQL 313
           EYFIGVKSI+I  K VPLN +LLKIDS+G+GGTKISTV+PYTVLESSIYNAV+K F   L
Sbjct: 259 EYFIGVKSIVINSKTVPLNTTLLKIDSNGVGGTKISTVDPYTVLESSIYNAVLKTFTTAL 318

Query: 314 RLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNG 373
           +      RVAAVAPFGACF+ K+ISSTR+GP VP+I+L+LQ+KKVIWRIFGANSMV V  
Sbjct: 319 K---NIPRVAAVAPFGACFNAKSISSTRLGPGVPSIELILQNKKVIWRIFGANSMVHVKD 378

Query: 374 GVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTS 430
            VLCLGFVDGGVNPRT+IVIGAHQIE+NLLEFDLA+SRLGFS+TLL R TTCANFNFTS
Sbjct: 379 DVLCLGFVDGGVNPRTSIVIGAHQIENNLLEFDLATSRLGFSATLLGRMTTCANFNFTS 433

BLAST of MC05g0552 vs. ExPASy TrEMBL
Match: A0A2I4GSL3 (probable aspartic proteinase GIP2 OS=Juglans regia OX=51240 GN=LOC109010481 PE=4 SV=2)

HSP 1 Score: 612 bits (1579), Expect = 4.38e-217
Identity = 309/424 (72.88%), Postives = 351/424 (82.78%), Query Frame = 0

Query: 11  FFFTSISATTSFRPKSLVLPVIKDPS-LQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNH 70
           F F SI+ T +FRPK+LVLPV KDPS LQY+ +L QRTP VP+KLT+DLGGQFLWVDC+ 
Sbjct: 20  FIFPSIAQTLTFRPKALVLPVSKDPSSLQYLAKLNQRTPLVPVKLTLDLGGQFLWVDCDQ 79

Query: 71  SYVSSTYKPARCRSAQCSLANSRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVAS 130
            YVSSTY PARCRSAQCSLA + +CGECFS PKPGCNN+TC   PDNTV    T GE+A 
Sbjct: 80  GYVSSTYNPARCRSAQCSLAKASSCGECFSAPKPGCNNNTCGLFPDNTVTRTATSGELAQ 139

Query: 131 DVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAF 190
           DVVS+QS+DGSNP RVVS+P FLF CG TFLLEGL  GV GMAG GRTRISLP QFSAAF
Sbjct: 140 DVVSIQSTDGSNPSRVVSVPKFLFTCGATFLLEGLTSGVKGMAGLGRTRISLPSQFSAAF 199

Query: 191 SFNRKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGD 250
           SF RKFA+CLSSST A G +FFG  G Y LLPN+++S +LIYTPL++NPVSTA  +  GD
Sbjct: 200 SFYRKFAICLSSSTRANGVVFFG-KGPYVLLPNMDVSESLIYTPLILNPVSTASVYSQGD 259

Query: 251 RSAEYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFV 310
            SAEYFIGVKSI I EK VPLN+SLL I+  G GGTKISTVNP+TVLE++IYNAV+  FV
Sbjct: 260 SSAEYFIGVKSIKIKEKRVPLNSSLLSINREGFGGTKISTVNPFTVLETTIYNAVINAFV 319

Query: 311 EQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVE 370
           ++L       RVA+VAPFGACF+ KNI STRVGPAVP ID VLQS+ V WRIFGANSMV+
Sbjct: 320 KELP---NVPRVASVAPFGACFNSKNIGSTRVGPAVPQIDFVLQSQTVYWRIFGANSMVQ 379

Query: 371 VNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNF 430
           V+  VLCLGFVDGG NPRT+IVIG HQ+EDNLL+FDLA+SRLGFSS+LLFRQTTCANFNF
Sbjct: 380 VSDDVLCLGFVDGGSNPRTSIVIGGHQLEDNLLQFDLATSRLGFSSSLLFRQTTCANFNF 439

Query: 431 TSNA 432
           TSNA
Sbjct: 440 TSNA 439

BLAST of MC05g0552 vs. ExPASy TrEMBL
Match: A0A0A0L5N4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G149980 PE=4 SV=1)

HSP 1 Score: 606 bits (1563), Expect = 9.23e-215
Identity = 302/416 (72.60%), Postives = 346/416 (83.17%), Query Frame = 0

Query: 18  ATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNHSYVSSTYK 77
           A TSFRPKSL+LPV K PS QYITQ+RQRTP VP+KLTVDLGGQF+WVDC+  YVSS+YK
Sbjct: 21  AATSFRPKSLLLPVTKHPSGQYITQIRQRTPLVPVKLTVDLGGQFMWVDCDRGYVSSSYK 80

Query: 78  PARCRSAQCSLANSRTCGECFSPPKPGCNNDTCSHLPDNTVVGLGTIGEVASDVVSLQSS 137
           P RCRSAQCSL+ S +CG+CFSPP+PGCNN+TC H P NT++ L T GEV SDV+S+ S+
Sbjct: 81  PVRCRSAQCSLSKSTSCGDCFSPPRPGCNNNTCGHFPGNTIIQLSTSGEVTSDVLSVSST 140

Query: 138 DGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQFSAAFSFNRKFAV 197
           +G NP R VSIPNFLFVCG TFLLEGLAGGV GMAGFGRT ISLP QFSAAFSFNRKFAV
Sbjct: 141 NGFNPTRAVSIPNFLFVCGPTFLLEGLAGGVSGMAGFGRTGISLPSQFSAAFSFNRKFAV 200

Query: 198 CLSSSTAAPGAIFFGGNGSYFLLPNIEIS-TLIYTPLLINPVSTAGAFFDGDRSAEYFIG 257
           CLS ST +PG IF  GNG Y  L N++++ +L YTPL INPVSTAG    G++S+EYFIG
Sbjct: 201 CLSGSTRSPGVIF-SGNGPYHFLQNVDVTKSLTYTPLFINPVSTAGVSTSGEKSSEYFIG 260

Query: 258 VKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVVKIFVEQLRLQGE 317
           VKSI+   K VP+N +LLKIDS+G GGTKISTV+PYTVLESSIYNA+VK    +LR    
Sbjct: 261 VKSIVFNSKTVPINTTLLKIDSNGNGGTKISTVHPYTVLESSIYNALVKTITRELR---N 320

Query: 318 EIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIFGANSMVEVNGGVLCL 377
             RVAAVAPFG C+  K+  STR+GP +P+IDL+LQ+KKVIWRIFGANSMV+VN  VLCL
Sbjct: 321 IPRVAAVAPFGVCYKSKSFGSTRLGPGMPSIDLILQNKKVIWRIFGANSMVQVNEEVLCL 380

Query: 378 GFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQTTCANFNFTSNA 432
           GFVDGGV  RTAIVIGA+Q+EDNLLEFDLA+SRLGFSSTLL R TTCANFNFTS A
Sbjct: 381 GFVDGGVEARTAIVIGAYQMEDNLLEFDLATSRLGFSSTLLGRMTTCANFNFTSTA 432

BLAST of MC05g0552 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 8.7e-150
Identity = 279/431 (64.73%), Postives = 326/431 (75.64%), Query Frame = 0

Query: 4   FFFIFFFFFFTSISATTSFRPKSLVLPVIKDPS-LQYITQLRQRTPFVPLKLTVDLGGQF 63
           F  +  F F  S SA T FRPK+L+LPV KD S LQY T + QRTP VP  +  DLGG+ 
Sbjct: 8   FSVLLLFIFSLSSSAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGRE 67

Query: 64  LWVDCNHSYVSSTYKPARCRSAQCSLANSRTCGECFSPPKPGCNNDTCSHLPDNTVVGLG 123
           LWVDC+  YVSSTY+  RC SA CS A S +CG CFSPP+PGC+N+TC  +PDNTV G  
Sbjct: 68  LWVDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNTCGGIPDNTVTGTA 127

Query: 124 TIGEVASDVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLP 183
           T GE A DVVS+QS++GSNPGRVV IPN +F CG TFLL+GLA G  GMAG GR  I LP
Sbjct: 128 TSGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTVGMAGMGRHNIGLP 187

Query: 184 PQFSAAFSFNRKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEISTLIYTPLLINPVSTAG 243
            QF+AAFSF+RKFAVCL   T+  G  FF GNG Y  LP I+IS+L  TPLLINPVSTA 
Sbjct: 188 SQFAAAFSFHRKFAVCL---TSGKGVAFF-GNGPYVFLPGIQISSLQTTPLLINPVSTAS 247

Query: 244 AFFDGDRSAEYFIGVKSILIAEKNVPLNNSLLKID-SHGIGGTKISTVNPYTVLESSIYN 303
           AF  G++S+EYFIGV +I I EK VP+N +LLKI+ S GIGGTKIS+VNPYTVLESSIYN
Sbjct: 248 AFSQGEKSSEYFIGVTAIQIVEKTVPINPTLLKINASTGIGGTKISSVNPYTVLESSIYN 307

Query: 304 AVVKIFVEQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIF 363
           A    FV+Q   +  + RVA+V PFGACF  KN+  TR+G AVP I+LVL SK V+WRIF
Sbjct: 308 AFTSEFVKQAAARSIK-RVASVKPFGACFSTKNVGVTRLGYAVPEIELVLHSKDVVWRIF 367

Query: 364 GANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQT 423
           GANSMV V+  V+CLGFVDGGVN RT++VIG  Q+EDNL+EFDLAS++ GFSSTLL RQT
Sbjct: 368 GANSMVSVSDDVICLGFVDGGVNARTSVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQT 427

Query: 424 TCANFNFTSNA 433
            CANFNFTS A
Sbjct: 428 NCANFNFTSTA 433

BLAST of MC05g0552 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 498.8 bits (1283), Expect = 4.3e-141
Identity = 265/431 (61.48%), Postives = 313/431 (72.62%), Query Frame = 0

Query: 4   FFFIFFFFFFTSISATTSFRPKSLVLPVIKDPS-LQYITQLRQRTPFVPLKLTVDLGGQF 63
           F  +    F  S SA  SFRPK+L+LPV KDPS LQY T + QRTP VP  +  DLGG+ 
Sbjct: 9   FSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLGGRE 68

Query: 64  LWVDCNHSYVSSTYKPARCRSAQCSLANSRTCGECFSPPKPGCNNDTCSHLPDNTVVGLG 123
            WVDC+  YVS+TY+  RC SA CS A S  CG CFSPP+PGC+N+TC   PDN++ G  
Sbjct: 69  FWVDCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNTCGAFPDNSITGWA 128

Query: 124 TIGEVASDVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLP 183
           T GE A DVVS+QS++GSNPGR V IPN +F CG+T LL+GLA G  GMAG GR  I LP
Sbjct: 129 TSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKGAVGMAGMGRHNIGLP 188

Query: 184 PQFSAAFSFNRKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEISTLIYTPLLINPVSTAG 243
            QF+AAFSFNRKFAVCL   T+  G  FF GNG Y  LP I+IS L  TPLLINP +T  
Sbjct: 189 LQFAAAFSFNRKFAVCL---TSGRGVAFF-GNGPYVFLPGIQISRLQKTPLLINPGTTVF 248

Query: 244 AFFDGDRSAEYFIGVKSILIAEKNVPLNNSLLKID-SHGIGGTKISTVNPYTVLESSIYN 303
            F  G++S EYFIGV +I I EK +P++ +LLKI+ S GIGGTKIS+VNPYTVLESSIY 
Sbjct: 249 EFSKGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTGIGGTKISSVNPYTVLESSIYK 308

Query: 304 AVVKIFVEQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKVIWRIF 363
           A    F+ Q   +  + RVA+V PFGACF  KN+  TR+G AVP I LVL SK V+WRIF
Sbjct: 309 AFTSEFIRQAAARSIK-RVASVKPFGACFSTKNVGVTRLGYAVPEIQLVLHSKDVVWRIF 368

Query: 364 GANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLFRQT 423
           GANSMV V+  V+CLGFVDGGVNP  ++VIG  Q+EDNL+EFDLAS++ GFSSTLL RQT
Sbjct: 369 GANSMVSVSDDVICLGFVDGGVNPGASVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQT 428

Query: 424 TCANFNFTSNA 433
            CANFNFTS A
Sbjct: 429 NCANFNFTSTA 434

BLAST of MC05g0552 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 194.1 bits (492), Expect = 2.3e-49
Identity = 152/416 (36.54%), Postives = 203/416 (48.80%), Query Frame = 0

Query: 7   IFFFFFFTSI---SATTSFRPKSLVLPVIKD-PSLQYITQLRQRTPFVPLKLTVDLGGQF 66
           +FFF F +++    +  S     +V PV+KD P+ QY+ Q+R      P+KL VDL G  
Sbjct: 9   LFFFSFLSALIISKSQISDSVNGVVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSI 68

Query: 67  LWVDCNHSYVSSTYKPARCRSAQCSLANSRTCGECFSPPKPGCNNDTCSHLPDNTVVGLG 126
           LW DC+  +VSS+       S+ C  A         S       N  C  L  N   G+ 
Sbjct: 69  LWFDCSSRHVSSSRNLISGSSSGCLKAKVGNERVSSSSSSRKDQNADCELLVKNDAFGIT 128

Query: 127 TIGEVASDVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLP 186
             GE+ SDV+S+ S   ++PG V    + LF C   +LL GLA G  G+ G GR +ISLP
Sbjct: 129 ARGELFSDVMSVGSV--TSPGTV----DLLFACTPPWLLRGLASGAQGVMGLGRAQISLP 188

Query: 187 PQFSAAFSFNRKFAVCLSS-----STAAPGAIFFGGNGSYFLLPNIEISTLIYTPLLINP 246
            Q +A  +  R+  V LS      ST++   + FG   S          +L+YTPLL   
Sbjct: 189 SQLAAETNERRRLTVYLSPLNGVVSTSSVEEV-FGVAAS---------RSLVYTPLLTG- 248

Query: 247 VSTAGAFFDGDRSAEYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLES 306
                       S  Y I VKSI +  +         K+   G    ++STV PYT+LES
Sbjct: 249 -----------SSGNYVINVKSIRVNGE---------KLSVEGPLAVELSTVVPYTILES 308

Query: 307 SIYNAVVKIFVE-QLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKV 366
           SIY    K+F E   +  GE   V  VAPFG CF              P +DL LQS+ V
Sbjct: 309 SIY----KVFAEAYAKAAGEATSVPPVAPFGLCFTS--------DVDFPAVDLALQSEMV 368

Query: 367 IWRIFGANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGF 413
            WRI G N MV+V GGV C G VDGG +    IV+G  Q+E  +L+FDL +S +GF
Sbjct: 369 RWRIHGKNLMVDVGGGVRCSGIVDGGSSRVNPIVMGGLQLEGFILDFDLGNSMMGF 375

BLAST of MC05g0552 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 192.2 bits (487), Expect = 8.7e-49
Identity = 143/428 (33.41%), Postives = 209/428 (48.83%), Query Frame = 0

Query: 13  FTSISATTSFRPKS-LVLPVIK--DPSLQYITQLRQRTPFVPLKLTVDLGGQFLWVDCNH 72
           F SI A  + +  S  +LP+ K    +L Y T         P+ L +DLG    W+DC  
Sbjct: 11  FLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNLTWLDCRK 70

Query: 73  SYVSSTYKPARCRSAQCSLANSRTCGECFSPPKPGCNNDTCSH-----LPDNTVVGLGTI 132
               S+ +   C+S+ C            S P  GC   +C +     L  N VV     
Sbjct: 71  LKSLSSLRLVTCQSSTCK-----------SIPGNGCAGKSCLYKQPNPLGQNPVV----T 130

Query: 133 GEVASDVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRISLPPQ 192
           G V  D  SL ++DG      VS+ +F F C     L+GL   V G+        S   Q
Sbjct: 131 GRVVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLALSPGSSSFTKQ 190

Query: 193 FSAAFSFNRKFAVCLSSSTAAPGAIFFGGNGSYFLLPNIEISTLIYTPLLINPVSTAGAF 252
            ++AF+   KF++CL SS    G   F   G ++ +P    S         NP+      
Sbjct: 191 VTSAFNVIPKFSLCLPSS----GTGHFYIAGIHYFIPPFNSSD--------NPIPRTLTP 250

Query: 253 FDGDRSAEYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLESSIYNAVV 312
             G  S +Y I VKSI +    + LN  LL       GG K+STV  YTVL++ IYNA+ 
Sbjct: 251 IKGTDSGDYLITVKSIYVGGTALKLNPDLL------TGGAKLSTVVHYTVLQTDIYNALA 310

Query: 313 KIFVEQLRLQGEEIRVAAVAPFGACFD----GKNISSTRVGPAVPTIDLVLQSK--KVIW 372
           + F  + +  G   +V +VAPF  CFD    GKN+++   GP VP I++ L  +  +V W
Sbjct: 311 QSFTLKAKAMG-IAKVPSVAPFKHCFDSRTAGKNLTA---GPNVPVIEIGLPGRIGEVKW 370

Query: 373 RIFGANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTLLF 427
             +GAN++V+V   V+CL F+DGG  P+  +VIG HQ++D++LEFD + + L FS +LL 
Sbjct: 371 GFYGANTVVKVKETVMCLAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGTVLAFSESLLL 401

BLAST of MC05g0552 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 173.3 bits (438), Expect = 4.2e-43
Identity = 141/428 (32.94%), Postives = 203/428 (47.43%), Query Frame = 0

Query: 4   FFFIFFFFFFTSISATTSFRPKSLVLPVIKDPSLQYITQLRQRTPFVPLKLTVDLGGQF- 63
           F  +   F + + ++ +  + +S + P+ KD +    T           K  +DL G   
Sbjct: 7   FLLLSLVFLYLANTSHSLRKFQSFLHPIYKDTAKNIYTIPLSIGSTSSEKFVLDLNGAAP 66

Query: 64  LWVDCNHSYVSSTYKPARCRSAQCSLANSR-TC-GECFSPPKPGCNNDTCSHLPDNTVVG 123
           L  +C  +  S+TY P RC S +C  AN    C     +  +  C +   S L  +TV  
Sbjct: 67  LLQNCPTAAKSTTYHPIRCGSTRCKYANPNFPCPNNVIAKKRTVCLSSDNSRLFRDTVPL 126

Query: 124 LGTIGEVASDVVSLQSSDGSNPGRVVSIPNFLFVCGTTFLLEGLAGGVHGMAGFGRTRIS 183
           L T   V +    + SS                   T    +G         G   T +S
Sbjct: 127 LYTFNGVYTRDSEMSSS------------------LTLTCTDGAPALKQRTIGLANTHLS 186

Query: 184 LPPQFSAAFSFNRKFAVCLSSS--TAAPGAIFFGGNGSYFLLPNIEISTLIY--TPLLIN 243
           +P Q  + +    K A+CL S+  + +     + G G Y+ LP  +  + I+  TPL+ N
Sbjct: 187 IPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIGKGEYYYLPYDKDVSKIFASTPLIGN 246

Query: 244 PVSTAGAFFDGDRSAEYFIGVKSILIAEKNVPLNNSLLKIDSHGIGGTKISTVNPYTVLE 303
                       +S EY I VKSI I  K VP+            G TKIST+ PYTV +
Sbjct: 247 -----------GKSGEYLIDVKSIQIGAKTVPI----------PYGATKISTLAPYTVFQ 306

Query: 304 SSIYNAVVKIFVEQLRLQGEEIRVAAVAPFGACFDGKNISSTRVGPAVPTIDLVLQSKKV 363
           +S+Y A++  F E +++     +  AV PFGACF          G  VP IDLVL S   
Sbjct: 307 TSLYKALLTAFTENIKI----AKAPAVKPFGACFYSNG------GRGVPVIDLVL-SGGA 366

Query: 364 IWRIFGANSMVEVNGGVLCLGFVDGGVNPRTAIVIGAHQIEDNLLEFDLASSRLGFSSTL 423
            WRI+G+NS+V+VN  V+CLGFVDGGV P+  IVIG  Q+EDNL+EFDL +S+  FSS+L
Sbjct: 367 KWRIYGSNSLVKVNKNVVCLGFVDGGVKPKYPIVIGGFQMEDNLVEFDLEASKFSFSSSL 384

Query: 424 LFRQTTCA 425
           L   T+C+
Sbjct: 427 LLHNTSCS 384