MC05g0493 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0493
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionSulfate adenylyltransferase
LocationMC05: 3669529 .. 3672427 (-)
RNA-Seq ExpressionMC05g0493
SyntenyMC05g0493
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAATTCTACACATCAAATTAAAAATTCATCCTATTCACACGTCACAACTCATAGAAATATTATTATATTAACTAATGAATAATTATATAATATAAAGAAAAAAAGACTAAAATTCCTTTAAAATTAGGGATAAAATATAAACGTTAAAATTGACATTAAAGAGAGAACCATATTTATTTATAGCCTTTATTTTTCCACCAAAAAAATACCCATCATCAGTTTGAATTGATGGGTCCCACTCCCAAATGACAGCTTTAGAATTAAAAAAACTCCAATGAAAGACCAATTTTGAAAAATCAAAAAGCGAGAAACAAACAAAAACAATGACAGGGGAAAAGGGTGTCCAGATTCATCCTCCGAACCCCAAAAGGAAAAAACTCAACACAAGGAATCTTCTTCGCCCAATTGCGCCCATAAATATTCCAACTCCAATTCTCCTCCTCTCTTATAAGTTAATAACTTTTATCAAACATCCCATCCCACTATCTCCGATTAATCCCTCCCTTCCATTTCGTCTTCTTCATCAATTTCTGAGTTCAGTTTCTCCATAACCTCGCTTTGAGGCCTTGGTCTCCATCTCAGATGGCGTCCATGGCGACTCGTTTCACCAAATCCTCATCCCCATTTCATTCTATTACCAGAACATCCAATCCCCATTTCGCCGCTCCGGTGAAAATCTCAATTTCTCGGAGCTCCAAGGCGAGGACCCCCCTCCGGCTCCGGGTCTCGGCCGGGTTGATCGAACCGGACGGCGGAAGGCTGGTGGAGCTTCTGGTTGAGGAGCCTCTGAGGGGTTTGAAGAAGAGGGAGGCCTTATCCTTGCCCAGAATCGAGCTCTCCAGCATCGATATCCAGTGGGTTCATGTGCTCAGCGAAGGCTGGGCCAGCCCTCTCACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCCCTCAGGCTCCCGGATGGGTCTGTGGCGAATATGTCAGTGCCCATTGTTCTCGCCATTGACGATGCCCAAAAGCACCGCATCGCCGACTCCACTTCTGTCGCTCTCTTTGACGCCAACAACAATCCCATCGCCATTCTCAAAGAGTGAGATTTCTCTACCCATTCACTTAATTCCTTATGAATCTATCTCTAATATATCTCTGCGCTATGTTTTGTATGAGCAATTCGTTGTTAAGTTTTACTTCTGTGTTAGTCTGCAAAAGAAAATAACACATGCATGTAATTCTAATTCATTTGAAGGTCAGTTCTCTAATTAGCTGAGTGAGATCTCCTACCAGAAAGAACCCCCTCTTTTTAACTTTTGACAATACTAAGATTTATACCAAGGTTCAAATATACGTAGAATTTCATCCGCATCTGTGTTTCTCTTTGTCATAATATTCCAGAGCTCAGATTTTCTCTGTTTGGAATTGGATTTCACATATTTTGTCTCTTGGGACTATACCATTGATAGCACGAGGAAGATTTTGACTATTTGTTGGTGTCGGCCTTCTGAAGATTTTGATTAACATTCACTTTGTTGGAGATCAGATTTGTTATAACCATACTTGTTTACTGTTTAGTTCCAACTCCCATTGAAGAGTATATGCTGATAGAGTCTAATAGTTGCAGTAACTTTTCTGCAGTGGTCTTTTCATACTTTCTAATAAGTTCTCTAATGTCTTGTTTGGATTTATCAGTATTGAGATCTACAAGCACCCTGAAGAAGAAAGGATAGCAAGAACCTGGGGCACCACTGCCCCAGGACTCCCTTACGTTGATCAAGCTATAACCAATGCTGGTAATTGGCTGATTGGGGGTGATTTGGAGGTTATAGAACCAATCAAGTACCATGATGGTCTGGACCGTTTCCGACAGTCACCTGCAGAACTACGGGAGGAATTTACCCGGCGAAATGCAGATGCAGTATTTGCATTCCAACTTCGCAATCCAGTCCATAACGGGCATGCTTTACTAATGACTGACACCCGCCGTCGGCTGCTCGATATGGGGTACAAGAACCCCATTTTGTTGCTTCATCCTCTGGGTGGCTACACCAAGGCAGATGATGTTCCACTTAGTTGGCGAATGAAGCAACATGAGAAGGTGCCTAATTTTGAACCATTTTTCCTACTCCTTTTGTTGATTGTACGAGGAAAGCTCTAACTTTATGAACTGCGTCGATTCTATCAGGTGCTTGAGGATGGTGTTCTTGATCCAGAGACAACGGTGGTCTCCATATTTCCATCTCCAATGCACTATGCAGGGCCAACAGAGGTGCAGTGGCATGCAAAGGCGAGAATCAATGCAGGGGCTAACTTTTACATTGTTGGCCGTGACCCTGCTGGAATGGGTCATCCTACTGAGAAAAGGGATCTGTATGATGCTGACCATGGGAAGAAAGTACTGAGCATGGCACCTGGACTGGAGCGGCTAAACATCCTTCCTTTCAGAGTAAGCTTATTTAGAATCCTCTGCCATCAAACTTGGTGAACTTAACTCGGTTAAGTAAAGGGAGTTACTCATCTATTTTGTTTCACATTCAGGTTGCTGCTTATGATAAAACTCAGGGCAAAATGGCCTTTTTCGATCCCTCGAGACCTCAGGATTTCCTTTTCATATCCGGCACCAAGGTAACTACTCACTCATATTATAATTCTAGACATTAGACTGCCATTTCCAATATAACATCAAGAACTAAATTGCTACTTTGGTAAAAAAAAAAAAAAGCACCATAAATCTTATCTTACTGTGATTTGGTTGTAAACCTGTGGTTGTATGATGCAGATGAGAACACTAGCAAAGAACAAAGAGAATCCACCAGAAGGGTTTATGTGCCCAGGGGGATGGAAGGTGTTGGTTGAATATTATGACAGTTTGGTACCTGCAAGCAATGACAGACTTCCTGAACCT

mRNA sequence

CTTAATTCTACACATCAAATTAAAAATTCATCCTATTCACACGTCACAACTCATAGAAATATTATTATATTAACTAATGAATAATTATATAATATAAAGAAAAAAAGACTAAAATTCCTTTAAAATTAGGGATAAAATATAAACGTTAAAATTGACATTAAAGAGAGAACCATATTTATTTATAGCCTTTATTTTTCCACCAAAAAAATACCCATCATCAGTTTGAATTGATGGGTCCCACTCCCAAATGACAGCTTTAGAATTAAAAAAACTCCAATGAAAGACCAATTTTGAAAAATCAAAAAGCGAGAAACAAACAAAAACAATGACAGGGGAAAAGGGTGTCCAGATTCATCCTCCGAACCCCAAAAGGAAAAAACTCAACACAAGGAATCTTCTTCGCCCAATTGCGCCCATAAATATTCCAACTCCAATTCTCCTCCTCTCTTATAAGTTAATAACTTTTATCAAACATCCCATCCCACTATCTCCGATTAATCCCTCCCTTCCATTTCGTCTTCTTCATCAATTTCTGAGTTCAGTTTCTCCATAACCTCGCTTTGAGGCCTTGGTCTCCATCTCAGATGGCGTCCATGGCGACTCGTTTCACCAAATCCTCATCCCCATTTCATTCTATTACCAGAACATCCAATCCCCATTTCGCCGCTCCGGTGAAAATCTCAATTTCTCGGAGCTCCAAGGCGAGGACCCCCCTCCGGCTCCGGGTCTCGGCCGGGTTGATCGAACCGGACGGCGGAAGGCTGGTGGAGCTTCTGGTTGAGGAGCCTCTGAGGGGTTTGAAGAAGAGGGAGGCCTTATCCTTGCCCAGAATCGAGCTCTCCAGCATCGATATCCAGTGGGTTCATGTGCTCAGCGAAGGCTGGGCCAGCCCTCTCACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCCCTCAGGCTCCCGGATGGGTCTGTGGCGAATATGTCAGTGCCCATTGTTCTCGCCATTGACGATGCCCAAAAGCACCGCATCGCCGACTCCACTTCTGTCGCTCTCTTTGACGCCAACAACAATCCCATCGCCATTCTCAAAGATATTGAGATCTACAAGCACCCTGAAGAAGAAAGGATAGCAAGAACCTGGGGCACCACTGCCCCAGGACTCCCTTACGTTGATCAAGCTATAACCAATGCTGGTAATTGGCTGATTGGGGGTGATTTGGAGGTTATAGAACCAATCAAGTACCATGATGGTCTGGACCGTTTCCGACAGTCACCTGCAGAACTACGGGAGGAATTTACCCGGCGAAATGCAGATGCAGTATTTGCATTCCAACTTCGCAATCCAGTCCATAACGGGCATGCTTTACTAATGACTGACACCCGCCGTCGGCTGCTCGATATGGGGTACAAGAACCCCATTTTGTTGCTTCATCCTCTGGGTGGCTACACCAAGGCAGATGATGTTCCACTTAGTTGGCGAATGAAGCAACATGAGAAGGTGCTTGAGGATGGTGTTCTTGATCCAGAGACAACGGTGGTCTCCATATTTCCATCTCCAATGCACTATGCAGGGCCAACAGAGGTGCAGTGGCATGCAAAGGCGAGAATCAATGCAGGGGCTAACTTTTACATTGTTGGCCGTGACCCTGCTGGAATGGGTCATCCTACTGAGAAAAGGGATCTGTATGATGCTGACCATGGGAAGAAAGTACTGAGCATGGCACCTGGACTGGAGCGGCTAAACATCCTTCCTTTCAGAGTTGCTGCTTATGATAAAACTCAGGGCAAAATGGCCTTTTTCGATCCCTCGAGACCTCAGGATTTCCTTTTCATATCCGGCACCAAGATGAGAACACTAGCAAAGAACAAAGAGAATCCACCAGAAGGGTTTATGTGCCCAGGGGGATGGAAGGTGTTGGTTGAATATTATGACAGTTTGGTACCTGCAAGCAATGACAGACTTCCTGAACCT

Coding sequence (CDS)

ATGGCGTCCATGGCGACTCGTTTCACCAAATCCTCATCCCCATTTCATTCTATTACCAGAACATCCAATCCCCATTTCGCCGCTCCGGTGAAAATCTCAATTTCTCGGAGCTCCAAGGCGAGGACCCCCCTCCGGCTCCGGGTCTCGGCCGGGTTGATCGAACCGGACGGCGGAAGGCTGGTGGAGCTTCTGGTTGAGGAGCCTCTGAGGGGTTTGAAGAAGAGGGAGGCCTTATCCTTGCCCAGAATCGAGCTCTCCAGCATCGATATCCAGTGGGTTCATGTGCTCAGCGAAGGCTGGGCCAGCCCTCTCACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCCCTCAGGCTCCCGGATGGGTCTGTGGCGAATATGTCAGTGCCCATTGTTCTCGCCATTGACGATGCCCAAAAGCACCGCATCGCCGACTCCACTTCTGTCGCTCTCTTTGACGCCAACAACAATCCCATCGCCATTCTCAAAGATATTGAGATCTACAAGCACCCTGAAGAAGAAAGGATAGCAAGAACCTGGGGCACCACTGCCCCAGGACTCCCTTACGTTGATCAAGCTATAACCAATGCTGGTAATTGGCTGATTGGGGGTGATTTGGAGGTTATAGAACCAATCAAGTACCATGATGGTCTGGACCGTTTCCGACAGTCACCTGCAGAACTACGGGAGGAATTTACCCGGCGAAATGCAGATGCAGTATTTGCATTCCAACTTCGCAATCCAGTCCATAACGGGCATGCTTTACTAATGACTGACACCCGCCGTCGGCTGCTCGATATGGGGTACAAGAACCCCATTTTGTTGCTTCATCCTCTGGGTGGCTACACCAAGGCAGATGATGTTCCACTTAGTTGGCGAATGAAGCAACATGAGAAGGTGCTTGAGGATGGTGTTCTTGATCCAGAGACAACGGTGGTCTCCATATTTCCATCTCCAATGCACTATGCAGGGCCAACAGAGGTGCAGTGGCATGCAAAGGCGAGAATCAATGCAGGGGCTAACTTTTACATTGTTGGCCGTGACCCTGCTGGAATGGGTCATCCTACTGAGAAAAGGGATCTGTATGATGCTGACCATGGGAAGAAAGTACTGAGCATGGCACCTGGACTGGAGCGGCTAAACATCCTTCCTTTCAGAGTTGCTGCTTATGATAAAACTCAGGGCAAAATGGCCTTTTTCGATCCCTCGAGACCTCAGGATTTCCTTTTCATATCCGGCACCAAGATGAGAACACTAGCAAAGAACAAAGAGAATCCACCAGAAGGGTTTATGTGCCCAGGGGGATGGAAGGTGTTGGTTGAATATTATGACAGTTTGGTACCTGCAAGCAATGACAGACTTCCTGAACCT

Protein sequence

MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRLVELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFNSLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP
Homology
BLAST of MC05g0493 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 2.1e-223
Identity = 380/464 (81.90%), Postives = 413/464 (89.01%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVS---AGLIEPDG 60
           MASMA   +K+      +T+ S+P+   P   ++S  SK+   LR RV    AGLI PDG
Sbjct: 1   MASMAAVLSKTPFLSQPLTK-SSPNSDLPF-AAVSFPSKS---LRRRVGSIRAGLIAPDG 60

Query: 61  GRLVELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTL 120
           G+LVEL+VEEP R  KK EA  LPR+EL++ID+QW+HVLSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 120

Query: 121 HFNSLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPE 180
           HFNSLRL DGSV NMSVPIVLAIDD QK RI +ST VALF+++ NP+AIL DIEIYKHP+
Sbjct: 121 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEF 240
           EERIARTWGTTAPGLPYVD+AITNAGNWLIGGDLEV+EP+KY+DGLDRFR SPAELR+E 
Sbjct: 181 EERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKEL 240

Query: 241 TRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSW 300
            +RNADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGG+TKADDVPL W
Sbjct: 241 EKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDW 300

Query: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHP EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS
Sbjct: 361 GHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420

Query: 421 GTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPE 462
           GTKMRTLAKN ENPP+GFMCPGGWKVLV+YY+SL PA N RLPE
Sbjct: 421 GTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of MC05g0493 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 1.9e-216
Identity = 364/461 (78.96%), Postives = 399/461 (86.55%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASM+T F K +S        S+   +    IS   +SK R+   + V AGLIEPDGG+L
Sbjct: 1   MASMSTVFPKPTSFISQPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGKL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           V+L+V EP R  KK EA  LPR+ L++ID+QW+HVLSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
            L L DGSV NMSVPIVLAIDD QK  I +S  V+L D+++NPIAIL DIEIYKHP+EER
Sbjct: 121 LLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYV++AITNAG+WLIGGDLEV+EP+KY+DGLDRFR SP ELR+E  +R
Sbjct: 181 IARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
            ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGG+TKADDVPLSWRMK
Sbjct: 241 GADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPE 462
           MR LAKN+ENPP+GFMCPGGWKVLV+YYDSL    N +LPE
Sbjct: 421 MRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of MC05g0493 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 4.3e-208
Identity = 359/465 (77.20%), Postives = 394/465 (84.73%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFA--APVKISISRSSKARTPLRLRVSAGLIEPDGG 60
           MAS A     S SPF S     N H +  AP  IS+    +  +   L V +GLIEPDGG
Sbjct: 1   MASSAAAIV-SGSPFRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPDGG 60

Query: 61  RLVELLVEEPLRGLKKREALSLP-RIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTL 120
           +L+ L+VEE  R + K EA ++P RI+L+ +D++WVHVLSEGWASPL GFMR+SEFLQTL
Sbjct: 61  KLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQTL 120

Query: 121 HFNSLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPE 180
           HFNS RL DGSV NMSVPIVLAIDD QK RI DS  V L D+  NPIAIL DIEIYKHP+
Sbjct: 121 HFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEF 240
           EERIARTWGTTA GLPY ++AIT AGNWLIGGDL+V+EPIKY+DGLDRFR SP++LREEF
Sbjct: 181 EERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLREEF 240

Query: 241 TRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSW 300
            RR ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNP+LLL+PLGG+TKADDVPLSW
Sbjct: 241 IRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPLSW 300

Query: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           RM+QHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHPTEKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSR QDFLFIS
Sbjct: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLFIS 420

Query: 421 GTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSL-VPASNDRLPE 462
           GTKMR LAK KENPP+GFMCP GWKVLV+YYDSL     N R+ E
Sbjct: 421 GTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGRVSE 464

BLAST of MC05g0493 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 2.7e-186
Identity = 309/445 (69.44%), Postives = 369/445 (82.92%), Query Frame = 0

Query: 7   RFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRLVELLVE 66
           R +K SS  + I+  S+ +    + +   R+   ++  ++ V + LI+PDGG LVEL+V 
Sbjct: 19  RNSKPSSFTNQISFLSSSNNNPFLNLVYKRNLTMQSVSKMTVKSSLIDPDGGELVELIVP 78

Query: 67  EPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFNSLRLPD 126
           E   G+KK E+ ++P+++L+ ID++WVHV+SEGWASPL GFMRE E+LQ+LHFNSLRL +
Sbjct: 79  ETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRLKN 138

Query: 127 GSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEERIARTWG 186
           G+  NMS+PIVLAIDD  K +I  S +VAL     + I  L+ +EIYKH +EERIARTWG
Sbjct: 139 GTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLRSVEIYKHNKEERIARTWG 198

Query: 187 TTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRRNADAVF 246
           TT+PGLPYV++ IT +GNWLIGGDLEV EPIKY+DGLD +R SP +LREEF  R ADAVF
Sbjct: 199 TTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSPKQLREEFDNRQADAVF 258

Query: 247 AFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMKQHEKVL 306
           AFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLLHPLGG+TKADDVPL  RM+QH KVL
Sbjct: 259 AFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKADDVPLDVRMEQHSKVL 318

Query: 307 EDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDL 366
           EDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDL
Sbjct: 319 EDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDL 378

Query: 367 YDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRTLAK 426
           YD DHGK+VLSMAPGLE+LNILPFRVAAYD  + KMAFFDPSR ++FLFISGTKMRT A+
Sbjct: 379 YDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAFFDPSRAKEFLFISGTKMRTYAR 438

Query: 427 NKENPPEGFMCPGGWKVLVEYYDSL 452
             ENPP+GFMCP GW VLV+YY+SL
Sbjct: 439 TGENPPDGFMCPSGWNVLVKYYESL 463

BLAST of MC05g0493 vs. ExPASy Swiss-Prot
Match: Q27128 (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase OS=Urechis caupo OX=6431 PE=2 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 7.7e-141
Identity = 239/387 (61.76%), Postives = 295/387 (76.23%), Query Frame = 0

Query: 62  ELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFNS 121
           EL V E      K E + LP +E++ +D QWV VLSEGWA+PLTGFMRE E+LQ+ HF  
Sbjct: 221 ELFVPESGLEHAKAEIVDLPTMEITKLDTQWVQVLSEGWATPLTGFMREREYLQSQHFGC 280

Query: 122 LRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEERI 181
           L   DG V N S+PIVL +  A K R+  S++ AL       IAIL+  E Y+H +EER 
Sbjct: 281 LL--DGGVTNQSIPIVLPVHTADKDRLEGSSAFAL-SYEGKRIAILRTPEFYEHRKEERC 340

Query: 182 ARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRRN 241
           +R +GT+  G PYV + I  +G+WL+GGDLEV+E I ++DGLD +R +P ELR +F   N
Sbjct: 341 SRQFGTSNAGQPYV-KMIMESGDWLVGGDLEVLERITWNDGLDEYRLTPNELRAKFRALN 400

Query: 242 ADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMKQ 301
           ADAVFAFQLRNPVHNGHALLMTDTRRRL + GYK P+LLLHPLGG+TK DDVPL+WRMKQ
Sbjct: 401 ADAVFAFQLRNPVHNGHALLMTDTRRRLTERGYKKPVLLLHPLGGWTKDDDVPLAWRMKQ 460

Query: 302 HEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPT 361
           H+ +L++ VLDP+ TV++IFPSPM YAGPTEVQWHAKAR++ GANFYIVGRDPAGM HP 
Sbjct: 461 HQAILDEKVLDPDYTVMAIFPSPMMYAGPTEVQWHAKARMSTGANFYIVGRDPAGMPHPE 520

Query: 362 EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKM 421
            K+DLY+A HG KVL+MAPGL +L I+PFRVAAY+KT+  M F+DP R  +F+FISGTKM
Sbjct: 521 TKQDLYNATHGAKVLTMAPGLTQLEIVPFRVAAYNKTKSAMDFYDPERHDEFMFISGTKM 580

Query: 422 RTLAKNKENPPEGFMCPGGWKVLVEYY 449
           R +A+  E PP GFM P  WK++VEYY
Sbjct: 581 RGMARAGETPPNGFMAPSAWKIMVEYY 603

BLAST of MC05g0493 vs. NCBI nr
Match: XP_022138069.1 (ATP sulfurylase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL
Sbjct: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462

BLAST of MC05g0493 vs. NCBI nr
Match: KAA0049323.1 (ATP sulfurylase 1 [Cucumis melo var. makuwa] >TYK17235.1 ATP sulfurylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 435/462 (94.16%), Postives = 447/462 (96.75%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFTK+ SPFHSI RTS  HF AP+K+SI+ S   +T LRLRVSAGLIEPDGGRL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHFGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKK+EALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFD+ NNPIA+LKDIEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 462

BLAST of MC05g0493 vs. NCBI nr
Match: XP_038895326.1 (ATP sulfurylase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 892 bits (2304), Expect = 0.0
Identity = 435/462 (94.16%), Postives = 445/462 (96.32%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFTK+ SPFHSI RTS  H  AP+K+SI+ S   ++  RLRVSAGLIEPDGGRL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIASSKSKKSHFRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLR LKK+EALSLPRIELSSIDIQW HVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRALKKKEALSLPRIELSSIDIQWAHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHRI DSTSVALFDANNNPIAILKDIEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIGDSTSVALFDANNNPIAILKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 462

BLAST of MC05g0493 vs. NCBI nr
Match: XP_008438607.1 (PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 891 bits (2303), Expect = 0.0
Identity = 434/462 (93.94%), Postives = 446/462 (96.54%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFTK+ SPFHSI RTS  H  AP+K+SI+ S   +T LRLRVSAGLIEPDGGRL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKK+EALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFD+ NNPIA+LKDIEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 462

BLAST of MC05g0493 vs. NCBI nr
Match: XP_004134113.1 (ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN56933.1 hypothetical protein Csa_009913 [Cucumis sativus])

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 434/462 (93.94%), Postives = 445/462 (96.32%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFT + SPFHSI RTS  H  APVK+SIS S   +T LRLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPVKVSISTSKSKKTHLRLRVSAGLIEPDGGKL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKK+EALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFD+ NNPIA+LK+IEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 462

BLAST of MC05g0493 vs. ExPASy TrEMBL
Match: A0A6J1C916 (Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009330 PE=4 SV=1)

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL
Sbjct: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462

BLAST of MC05g0493 vs. ExPASy TrEMBL
Match: A0A5A7U0F4 (Sulfate adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00860 PE=4 SV=1)

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 435/462 (94.16%), Postives = 447/462 (96.75%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFTK+ SPFHSI RTS  HF AP+K+SI+ S   +T LRLRVSAGLIEPDGGRL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHFGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKK+EALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFD+ NNPIA+LKDIEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 462

BLAST of MC05g0493 vs. ExPASy TrEMBL
Match: A0A1S3AWG3 (Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103483665 PE=4 SV=1)

HSP 1 Score: 891 bits (2303), Expect = 0.0
Identity = 434/462 (93.94%), Postives = 446/462 (96.54%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFTK+ SPFHSI RTS  H  AP+K+SI+ S   +T LRLRVSAGLIEPDGGRL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKK+EALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFD+ NNPIA+LKDIEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 462

BLAST of MC05g0493 vs. ExPASy TrEMBL
Match: A0A0A0L5D0 (Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G144230 PE=4 SV=1)

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 434/462 (93.94%), Postives = 445/462 (96.32%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASMATRFT + SPFHSI RTS  H  APVK+SIS S   +T LRLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPVKVSISTSKSKKTHLRLRVSAGLIEPDGGKL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKK+EALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFD+ NNPIA+LK+IEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 462

BLAST of MC05g0493 vs. ExPASy TrEMBL
Match: A0A6J1FPI4 (Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447315 PE=4 SV=1)

HSP 1 Score: 868 bits (2243), Expect = 0.0
Identity = 430/464 (92.67%), Postives = 442/464 (95.26%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSS-KARTP-LRLRVSAGLIEPDGG 60
           MAS ATRF K+ SPFHSI +TS  H  AP+KIS+   S KA+   LRLRVSAGLIEPDGG
Sbjct: 1   MASTATRFIKAPSPFHSIQKTSYTH--APIKISLPAGSFKAKKANLRLRVSAGLIEPDGG 60

Query: 61  RLVELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120
           RLVELLVEEPLRGLKK+EALSLPRI LSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH
Sbjct: 61  RLVELLVEEPLRGLKKKEALSLPRISLSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120

Query: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEE 180
           FNSLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFDANNNPIAI+KDIEIYKHP+E
Sbjct: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHHIGDSTSVALFDANNNPIAIMKDIEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240
           ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT
Sbjct: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240

Query: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWR 300
           RRNADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNP+LLLHPLGGYTKADDVPL WR
Sbjct: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGYTKADDVPLGWR 300

Query: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISG 420
           HPT+KRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISG
Sbjct: 361 HPTKKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420

Query: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462
           TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RL EP
Sbjct: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLAEP 462

BLAST of MC05g0493 vs. TAIR 10
Match: AT3G22890.1 (ATP sulfurylase 1 )

HSP 1 Score: 776.2 bits (2003), Expect = 1.5e-224
Identity = 380/464 (81.90%), Postives = 413/464 (89.01%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVS---AGLIEPDG 60
           MASMA   +K+      +T+ S+P+   P   ++S  SK+   LR RV    AGLI PDG
Sbjct: 1   MASMAAVLSKTPFLSQPLTK-SSPNSDLPF-AAVSFPSKS---LRRRVGSIRAGLIAPDG 60

Query: 61  GRLVELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTL 120
           G+LVEL+VEEP R  KK EA  LPR+EL++ID+QW+HVLSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 120

Query: 121 HFNSLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPE 180
           HFNSLRL DGSV NMSVPIVLAIDD QK RI +ST VALF+++ NP+AIL DIEIYKHP+
Sbjct: 121 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEF 240
           EERIARTWGTTAPGLPYVD+AITNAGNWLIGGDLEV+EP+KY+DGLDRFR SPAELR+E 
Sbjct: 181 EERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKEL 240

Query: 241 TRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSW 300
            +RNADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGG+TKADDVPL W
Sbjct: 241 EKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDW 300

Query: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHP EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS
Sbjct: 361 GHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420

Query: 421 GTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPE 462
           GTKMRTLAKN ENPP+GFMCPGGWKVLV+YY+SL PA N RLPE
Sbjct: 421 GTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of MC05g0493 vs. TAIR 10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 753.1 bits (1943), Expect = 1.4e-217
Identity = 364/461 (78.96%), Postives = 399/461 (86.55%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60
           MASM+T F K +S        S+   +    IS   +SK R+   + V AGLIEPDGG+L
Sbjct: 1   MASMSTVFPKPTSFISQPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGKL 60

Query: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           V+L+V EP R  KK EA  LPR+ L++ID+QW+HVLSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180
            L L DGSV NMSVPIVLAIDD QK  I +S  V+L D+++NPIAIL DIEIYKHP+EER
Sbjct: 121 LLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYV++AITNAG+WLIGGDLEV+EP+KY+DGLDRFR SP ELR+E  +R
Sbjct: 181 IARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
            ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGG+TKADDVPLSWRMK
Sbjct: 241 GADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPE 462
           MR LAKN+ENPP+GFMCPGGWKVLV+YYDSL    N +LPE
Sbjct: 421 MRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of MC05g0493 vs. TAIR 10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 725.3 bits (1871), Expect = 3.0e-209
Identity = 359/465 (77.20%), Postives = 394/465 (84.73%), Query Frame = 0

Query: 1   MASMATRFTKSSSPFHSITRTSNPHFA--APVKISISRSSKARTPLRLRVSAGLIEPDGG 60
           MAS A     S SPF S     N H +  AP  IS+    +  +   L V +GLIEPDGG
Sbjct: 1   MASSAAAIV-SGSPFRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPDGG 60

Query: 61  RLVELLVEEPLRGLKKREALSLP-RIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTL 120
           +L+ L+VEE  R + K EA ++P RI+L+ +D++WVHVLSEGWASPL GFMR+SEFLQTL
Sbjct: 61  KLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQTL 120

Query: 121 HFNSLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPE 180
           HFNS RL DGSV NMSVPIVLAIDD QK RI DS  V L D+  NPIAIL DIEIYKHP+
Sbjct: 121 HFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEF 240
           EERIARTWGTTA GLPY ++AIT AGNWLIGGDL+V+EPIKY+DGLDRFR SP++LREEF
Sbjct: 181 EERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLREEF 240

Query: 241 TRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSW 300
            RR ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNP+LLL+PLGG+TKADDVPLSW
Sbjct: 241 IRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPLSW 300

Query: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           RM+QHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420
           GHPTEKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSR QDFLFIS
Sbjct: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLFIS 420

Query: 421 GTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSL-VPASNDRLPE 462
           GTKMR LAK KENPP+GFMCP GWKVLV+YYDSL     N R+ E
Sbjct: 421 GTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGRVSE 464

BLAST of MC05g0493 vs. TAIR 10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 652.9 bits (1683), Expect = 1.9e-187
Identity = 309/445 (69.44%), Postives = 369/445 (82.92%), Query Frame = 0

Query: 7   RFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRLVELLVE 66
           R +K SS  + I+  S+ +    + +   R+   ++  ++ V + LI+PDGG LVEL+V 
Sbjct: 19  RNSKPSSFTNQISFLSSSNNNPFLNLVYKRNLTMQSVSKMTVKSSLIDPDGGELVELIVP 78

Query: 67  EPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFNSLRLPD 126
           E   G+KK E+ ++P+++L+ ID++WVHV+SEGWASPL GFMRE E+LQ+LHFNSLRL +
Sbjct: 79  ETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRLKN 138

Query: 127 GSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEERIARTWG 186
           G+  NMS+PIVLAIDD  K +I  S +VAL     + I  L+ +EIYKH +EERIARTWG
Sbjct: 139 GTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLRSVEIYKHNKEERIARTWG 198

Query: 187 TTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRRNADAVF 246
           TT+PGLPYV++ IT +GNWLIGGDLEV EPIKY+DGLD +R SP +LREEF  R ADAVF
Sbjct: 199 TTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSPKQLREEFDNRQADAVF 258

Query: 247 AFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMKQHEKVL 306
           AFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLLHPLGG+TKADDVPL  RM+QH KVL
Sbjct: 259 AFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKADDVPLDVRMEQHSKVL 318

Query: 307 EDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDL 366
           EDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDL
Sbjct: 319 EDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDL 378

Query: 367 YDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRTLAK 426
           YD DHGK+VLSMAPGLE+LNILPFRVAAYD  + KMAFFDPSR ++FLFISGTKMRT A+
Sbjct: 379 YDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAFFDPSRAKEFLFISGTKMRTYAR 438

Query: 427 NKENPPEGFMCPGGWKVLVEYYDSL 452
             ENPP+GFMCP GW VLV+YY+SL
Sbjct: 439 TGENPPDGFMCPSGWNVLVKYYESL 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIK92.1e-22381.90ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
O233241.9e-21678.96ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
Q9S7D84.3e-20877.20ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
Q438702.7e-18669.44ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
Q271287.7e-14161.76Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase OS=Urechis caupo OX=... [more]
Match NameE-valueIdentityDescription
XP_022138069.10.0100.00ATP sulfurylase 1, chloroplastic [Momordica charantia][more]
KAA0049323.10.094.16ATP sulfurylase 1 [Cucumis melo var. makuwa] >TYK17235.1 ATP sulfurylase 1 [Cucu... [more]
XP_038895326.10.094.16ATP sulfurylase 1, chloroplastic-like [Benincasa hispida][more]
XP_008438607.10.093.94PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo][more]
XP_004134113.10.093.94ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN56933.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
A0A6J1C9160.0100.00Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009330 PE=4 ... [more]
A0A5A7U0F40.094.16Sulfate adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AWG30.093.94Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103483665 PE=4 SV=1[more]
A0A0A0L5D00.093.94Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G144230 PE=4 SV=1[more]
A0A6J1FPI40.092.67Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447315 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G22890.11.5e-22481.90ATP sulfurylase 1 [more]
AT4G14680.11.4e-21778.96Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT5G43780.13.0e-20977.20Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT1G19920.11.9e-18769.44Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.400.10Sulfate adenylyltransferasecoord: 51..219
e-value: 2.6E-58
score: 198.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 1..462
NoneNo IPR availablePANTHERPTHR11055:SF41ATP SULFURYLASE 1, CHLOROPLASTICcoord: 1..462
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 225..448
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 55..446
e-value: 1.5E-138
score: 459.8
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 80..447
e-value: 9.95606E-166
score: 469.036
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 220..454
e-value: 2.0E-95
score: 320.5
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 53..215
e-value: 7.9E-45
score: 152.4
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 225..447
e-value: 1.5E-66
score: 223.9
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 53..221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0493.1MC05g0493.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity