MC05g0295 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0295 vs. ExPASy Swiss-Prot
Match: Q9QR71 (Protein LANA1 OS=Human herpesvirus 8 type P (isolate GK18) OX=868565 GN=LANA1 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 7.7e-04
Identity = 60/273 (21.98%), Postives = 134/273 (49.08%), Query Frame = 0

Query: 280 QQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQI 339
           Q    +D+++  E  +   +Q    +D QE+     Q  ++  +    Q+    + + Q 
Sbjct: 604 QDEQQQDEQQQDEQQQDEQQQDEQQQDEQEQQDEQQQDEQQQQDEQEQQEEQEQQEEQQQ 663

Query: 340 DEER--DQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESE 399
           DE++  +Q+++E+++   + +   Q+   + QDE ++  +     E+EQQE   + +E E
Sbjct: 664 DEQQQDEQQQDEQQQDEQEQQDEQQQDEQQQQDEQEQQEEQEQQEEQEQQEEQEQQEEQE 723

Query: 400 KERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQ 459
           +E    +E    +LEE  Q+L   + ++ + E +L EQ+     + +++EE E  ++E +
Sbjct: 724 QE----LEEQEQELEEQEQELEEQEQELEEQEQELEEQEQELEEQEQELEEQEQELEEQE 783

Query: 460 KELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 519
           +ELE  +Q   EE   ++   E E+    ++L+ + + L+   +   L E + +      
Sbjct: 784 QELEEQEQ-ELEEQEQELEEQEQELEEQEQELEEQEQELEEQEQEQELEEVEEQEQEQEE 843

Query: 520 EEISALFAKQQEQLKAMQRTLEDEENYENTSFD 551
           +E+  +  ++QEQ +  ++ LE+ E  E    +
Sbjct: 844 QELEEVEEQEQEQEEQEEQELEEVEEQEEQELE 871

BLAST of MC05g0295 vs. NCBI nr
Match: XP_022147056.1 (uncharacterized protein LOC111016086 isoform X1 [Momordica charantia])

HSP 1 Score: 1659 bits (4295), Expect = 0.0
Identity = 894/894 (100.00%), Postives = 894/894 (100.00%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60
           MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN
Sbjct: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60

Query: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120
           VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST
Sbjct: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120

Query: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180
           KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV
Sbjct: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180

Query: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240
           AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV
Sbjct: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240

Query: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300
           LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ
Sbjct: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300

Query: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360
           KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA
Sbjct: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360

Query: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420
           VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT
Sbjct: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420

Query: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL 480
           SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL
Sbjct: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL 480

Query: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540
           EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED
Sbjct: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540

Query: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600
           EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE
Sbjct: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600

Query: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660
           ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET
Sbjct: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660

Query: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720
           ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD
Sbjct: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720

Query: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780
           VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS
Sbjct: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780

Query: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840
           PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE
Sbjct: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840

Query: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 894
           NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG
Sbjct: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 894

BLAST of MC05g0295 vs. NCBI nr
Match: XP_022147057.1 (kinesin-like protein KIF15 isoform X2 [Momordica charantia])

HSP 1 Score: 1652 bits (4279), Expect = 0.0
Identity = 893/894 (99.89%), Postives = 893/894 (99.89%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60
           MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN
Sbjct: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60

Query: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120
           VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST
Sbjct: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120

Query: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180
           KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV
Sbjct: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180

Query: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240
           AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV
Sbjct: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240

Query: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300
           LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ
Sbjct: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300

Query: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360
           KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA
Sbjct: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360

Query: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420
           VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT
Sbjct: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420

Query: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL 480
           SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVK GAREEAWAKVSSLEL
Sbjct: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVK-GAREEAWAKVSSLEL 480

Query: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540
           EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED
Sbjct: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540

Query: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600
           EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE
Sbjct: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600

Query: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660
           ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET
Sbjct: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660

Query: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720
           ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD
Sbjct: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720

Query: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780
           VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS
Sbjct: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780

Query: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840
           PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE
Sbjct: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840

Query: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 894
           NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG
Sbjct: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 893

BLAST of MC05g0295 vs. NCBI nr
Match: XP_022924345.1 (uncharacterized protein LOC111431865 isoform X1 [Cucurbita moschata] >XP_022924346.1 uncharacterized protein LOC111431865 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1296 bits (3354), Expect = 0.0
Identity = 724/917 (78.95%), Postives = 792/917 (86.37%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTP---------------------- 60
           MD DD   N ++ KS ALPK G+GSQSS SH+ PP TP                      
Sbjct: 1   MDGDDA--NPETPKSTALPKHGSGSQSSSSHIHPPHTPHSPPRPPPLNSVDETRSNKPLS 60

Query: 61  -REFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADS 120
            REF+LSVASKIASQPLQN+ SNVWGVLTAIS NARKRQQG+NILLT+DEH LGRV  DS
Sbjct: 61  PREFVLSVASKIASQPLQNFDSNVWGVLTAISGNARKRQQGINILLTDDEHCLGRVAPDS 120

Query: 121 RYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180
           RYQI+SNS+SA HCRIYRK I  +DACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG
Sbjct: 121 RYQIDSNSVSAKHCRIYRKSI--EDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180

Query: 181 DIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPI 240
           DIISLAAAPQHEVAFAFVYREV AFTSSS GG AKRKAE+ VSENKRLRGLGIGAPDGPI
Sbjct: 181 DIISLAAAPQHEVAFAFVYREVAAFTSSSGGGSAKRKAEDFVSENKRLRGLGIGAPDGPI 240

Query: 241 SLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIA 300
           SLDDFRSLQRSNKELR+QLED VL ID+L  ENRAS EHHE EVKK++ES+SK Y+D++ 
Sbjct: 241 SLDDFRSLQRSNKELRKQLEDHVLMIDSLRNENRASVEHHECEVKKLKESISKSYEDQLT 300

Query: 301 KYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKG 360
           K QQ  D +Q+ELGE  R++SEQKH IEDLQERLSATAQSC EANE+INSQKASLS+LK 
Sbjct: 301 KMQQLIDDEQKELGEVQRISSEQKHVIEDLQERLSATAQSCNEANEIINSQKASLSELKV 360

Query: 361 QIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESE 420
           QIDEERDQRREEREKAAADLKAAV+KAHA AQDE+KR+SDAAS REREQQEVINKLQE++
Sbjct: 361 QIDEERDQRREEREKAAADLKAAVEKAHAVAQDELKRISDAASKREREQQEVINKLQEAD 420

Query: 421 KERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQ 480
           KERC  VETLRSKLEETRQKLV SDNKVRQLESQLGE+QLSC NERKKVEELE G+KELQ
Sbjct: 421 KERCFQVETLRSKLEETRQKLVMSDNKVRQLESQLGEEQLSCTNERKKVEELERGIKELQ 480

Query: 481 KELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540
           KELES KQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT
Sbjct: 481 KELESEKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540

Query: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVSLEAANGILLGERVQENYCNKS 600
           EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNV  + ANGILLG+ ++++YCNKS
Sbjct: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVPSQDANGILLGDNIRKDYCNKS 600

Query: 601 AKTSSAMSAQRFDPIQAETSTEEASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSD 660
            KTSSA SAQRF+P+QAETST+EASTEKND + RSQDC+NTQE EFTSADAGVKGGFGSD
Sbjct: 601 DKTSSATSAQRFEPVQAETSTDEASTEKNDCDFRSQDCQNTQEAEFTSADAGVKGGFGSD 660

Query: 661 IDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNE 720
           IDGVGTAPVLEGD VGTER+LETESPGVDG++N D NKG+ LAGET+C DDEG AGE +E
Sbjct: 661 IDGVGTAPVLEGDMVGTERILETESPGVDGDQNMDLNKGMILAGETICFDDEGCAGEMDE 720

Query: 721 QGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHG 780
           Q KM+ QEA CHSQTNQICDAV+  A EDTEA GTVRTADLLASEVAGSWA STAPSVHG
Sbjct: 721 QAKMVRQEAYCHSQTNQICDAVE--AVEDTEACGTVRTADLLASEVAGSWASSTAPSVHG 780

Query: 781 ENESPRSRGNEEEGGRALQDSNSPVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKH 840
           ENES RSRGNE  G  A+ DSNSP  G QS      ATRRN E+Q +SEMI+IVAPESK 
Sbjct: 781 ENESQRSRGNEGGGDGAIHDSNSP--GIQSTLFKPVATRRNSEYQTVSEMIRIVAPESKQ 840

Query: 841 FFGSREDGCERGGEESASSSDTENCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPEL 894
           FF SREDG E G ++S S SDT+ C+DNDDDA +N+E +AKEGRVSDSETQGVD +DP+L
Sbjct: 841 FFHSREDGRE-GEQDSTSGSDTDKCSDNDDDAHDNSESKAKEGRVSDSETQGVDAMDPKL 900

BLAST of MC05g0295 vs. NCBI nr
Match: XP_023527903.1 (uncharacterized protein LOC111790985 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023527904.1 uncharacterized protein LOC111790985 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023527905.1 uncharacterized protein LOC111790985 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1290 bits (3338), Expect = 0.0
Identity = 722/920 (78.48%), Postives = 795/920 (86.41%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPP----------------TPREFILS 60
           MD +D   N ++ KS ALPK G+GSQSS SH+ PP                 +PREF+LS
Sbjct: 1   MDGEDA--NPETPKSTALPKHGSGSQSSSSHIHPPHPPPLNSVDETRSNKPLSPREFVLS 60

Query: 61  VASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESN 120
           VASKIASQPLQN+ SNVWGVLTAIS NARKRQQG+NILLT+DEH LGRV  DSRYQI+SN
Sbjct: 61  VASKIASQPLQNFDSNVWGVLTAISGNARKRQQGINILLTDDEHCLGRVAPDSRYQIDSN 120

Query: 121 SISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAA 180
           S+SA HCRIYRK I  +DACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAA
Sbjct: 121 SVSAKHCRIYRKSI--EDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAA 180

Query: 181 APQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRS 240
           APQHEVAFAFVYREV AFTSSS  G AKRKAE+ VSENKRLRGLGIGAPDGPISLDDFRS
Sbjct: 181 APQHEVAFAFVYREVAAFTSSSGVGSAKRKAEDFVSENKRLRGLGIGAPDGPISLDDFRS 240

Query: 241 LQRSNKELRRQLEDQVLTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTD 300
           LQRSNKELR+QLED VL ID+L  ENRAS EHHE EVKK++ES+SK ++D++ K QQ  D
Sbjct: 241 LQRSNKELRKQLEDHVLMIDSLRNENRASVEHHECEVKKLKESISKSFEDQLTKMQQLID 300

Query: 301 RDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERD 360
            +Q+ELGE  R++SEQKH IEDLQERLSATAQSC EANE+INSQKASLS+LK QIDEERD
Sbjct: 301 DEQKELGEVQRISSEQKHVIEDLQERLSATAQSCNEANEIINSQKASLSELKVQIDEERD 360

Query: 361 QRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLV 420
           QRREEREKAAADLKAAV+KAHA AQDE+KR+SDAAS REREQQEVINKLQE+EKERC  V
Sbjct: 361 QRREEREKAAADLKAAVEKAHAVAQDELKRISDAASRREREQQEVINKLQEAEKERCFQV 420

Query: 421 ETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVK 480
           ETLRSKLEETRQKLV SDNKVRQLESQLGE+QLSC NERKKVEELE G+KELQKELES K
Sbjct: 421 ETLRSKLEETRQKLVMSDNKVRQLESQLGEEQLSCTNERKKVEELERGIKELQKELESEK 480

Query: 481 QGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALF 540
           QGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALF
Sbjct: 481 QGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALF 540

Query: 541 AKQQEQLKAMQRTLEDEENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAM 600
            KQQEQLKAMQRTLEDEENYENTSFDFDLNV  + ANGILLG+ ++++YCNKS KTSSAM
Sbjct: 541 GKQQEQLKAMQRTLEDEENYENTSFDFDLNVPSQDANGILLGDNIRKDYCNKSDKTSSAM 600

Query: 601 SAQRFDPIQAETSTEEASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTA 660
           SAQRF+P+QAETST+EASTEKND + RSQDC+NTQE EFTSADAGVKGGFGSDIDGVGTA
Sbjct: 601 SAQRFEPVQAETSTDEASTEKNDCDFRSQDCQNTQEAEFTSADAGVKGGFGSDIDGVGTA 660

Query: 661 PVLEGDAVGTERVLETESPGVDG-ERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIH 720
           PVLEGD VGTER+LETESPGVDG +RN D NKG+ LAGET+C DDEG AGE +EQ KM+ 
Sbjct: 661 PVLEGDVVGTERILETESPGVDGGDRNMDLNKGMILAGETICFDDEGCAGEMDEQAKMVR 720

Query: 721 QEACCHSQTNQICDAVD---------VDAREDTEAGGTVRTADLLASEVAGSWACSTAPS 780
           +EA CHS+TNQICDAVD         VDA EDTEA GTVRT+DLLASEVAGSWA STAPS
Sbjct: 721 REAYCHSRTNQICDAVDAVEDTEACAVDAVEDTEACGTVRTSDLLASEVAGSWASSTAPS 780

Query: 781 VHGENESPRSRGNEEEGGRALQDSNSPVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPE 840
           VHGENES RSRGNE  GG AL DSNSP  G++S      ATRRN E+Q +SEMI+IVAPE
Sbjct: 781 VHGENESQRSRGNEGGGGGALHDSNSP--GNRSTLFKPVATRRNSEYQTVSEMIRIVAPE 840

Query: 841 SKHFFGSREDGCERGGEESASSSDTENCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVD 894
           SK FF SREDG E G ++S S SDT+ C+DNDDDA +N+E +AK+GRVSDSETQGVD +D
Sbjct: 841 SKQFFRSREDGRE-GEQDSTSGSDTDKCSDNDDDAHDNSESKAKDGRVSDSETQGVDAMD 900

BLAST of MC05g0295 vs. NCBI nr
Match: XP_022924347.1 (uncharacterized protein LOC111431865 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1290 bits (3338), Expect = 0.0
Identity = 723/917 (78.84%), Postives = 791/917 (86.26%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTP---------------------- 60
           MD DD   N ++ KS ALPK G+GSQSS SH+ PP TP                      
Sbjct: 1   MDGDDA--NPETPKSTALPKHGSGSQSSSSHIHPPHTPHSPPRPPPLNSVDETRSNKPLS 60

Query: 61  -REFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADS 120
            REF+LSVASKIASQPLQN+ SNVWGVLTAIS NARKRQQG+NILLT+DEH LGRV  DS
Sbjct: 61  PREFVLSVASKIASQPLQNFDSNVWGVLTAISGNARKRQQGINILLTDDEHCLGRVAPDS 120

Query: 121 RYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180
           RYQI+SNS+SA HCRIYRK I  +DACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG
Sbjct: 121 RYQIDSNSVSAKHCRIYRKSI--EDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180

Query: 181 DIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPI 240
           DIISLAAAPQHEVAFAFVYREV AFTSSS GG AKRKAE+ VSENKRLRGLGIGAPDGPI
Sbjct: 181 DIISLAAAPQHEVAFAFVYREVAAFTSSSGGGSAKRKAEDFVSENKRLRGLGIGAPDGPI 240

Query: 241 SLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIA 300
           SLDDFRSLQRSNKELR+QLED VL ID+L  ENRAS EHHE EVKK++ES+SK Y+D++ 
Sbjct: 241 SLDDFRSLQRSNKELRKQLEDHVLMIDSLRNENRASVEHHECEVKKLKESISKSYEDQLT 300

Query: 301 KYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKG 360
           K QQ  D +Q+ELGE  R++SEQKH IEDLQERLSATAQSC EANE+INSQKASLS+LK 
Sbjct: 301 KMQQLIDDEQKELGEVQRISSEQKHVIEDLQERLSATAQSCNEANEIINSQKASLSELKV 360

Query: 361 QIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESE 420
           QIDEERDQRREEREKAAADLKAAV+KAHA AQDE+KR+SDAAS REREQQEVINKLQE++
Sbjct: 361 QIDEERDQRREEREKAAADLKAAVEKAHAVAQDELKRISDAASKREREQQEVINKLQEAD 420

Query: 421 KERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQ 480
           KERC  VETLRSKLEETRQKLV SDNKVRQLESQLGE+QLSC NERKKVEELE G+KELQ
Sbjct: 421 KERCFQVETLRSKLEETRQKLVMSDNKVRQLESQLGEEQLSCTNERKKVEELERGIKELQ 480

Query: 481 KELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540
           KELES K GAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT
Sbjct: 481 KELESEK-GAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540

Query: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVSLEAANGILLGERVQENYCNKS 600
           EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNV  + ANGILLG+ ++++YCNKS
Sbjct: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVPSQDANGILLGDNIRKDYCNKS 600

Query: 601 AKTSSAMSAQRFDPIQAETSTEEASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSD 660
            KTSSA SAQRF+P+QAETST+EASTEKND + RSQDC+NTQE EFTSADAGVKGGFGSD
Sbjct: 601 DKTSSATSAQRFEPVQAETSTDEASTEKNDCDFRSQDCQNTQEAEFTSADAGVKGGFGSD 660

Query: 661 IDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNE 720
           IDGVGTAPVLEGD VGTER+LETESPGVDG++N D NKG+ LAGET+C DDEG AGE +E
Sbjct: 661 IDGVGTAPVLEGDMVGTERILETESPGVDGDQNMDLNKGMILAGETICFDDEGCAGEMDE 720

Query: 721 QGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHG 780
           Q KM+ QEA CHSQTNQICDAV+  A EDTEA GTVRTADLLASEVAGSWA STAPSVHG
Sbjct: 721 QAKMVRQEAYCHSQTNQICDAVE--AVEDTEACGTVRTADLLASEVAGSWASSTAPSVHG 780

Query: 781 ENESPRSRGNEEEGGRALQDSNSPVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKH 840
           ENES RSRGNE  G  A+ DSNSP  G QS      ATRRN E+Q +SEMI+IVAPESK 
Sbjct: 781 ENESQRSRGNEGGGDGAIHDSNSP--GIQSTLFKPVATRRNSEYQTVSEMIRIVAPESKQ 840

Query: 841 FFGSREDGCERGGEESASSSDTENCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPEL 894
           FF SREDG E G ++S S SDT+ C+DNDDDA +N+E +AKEGRVSDSETQGVD +DP+L
Sbjct: 841 FFHSREDGRE-GEQDSTSGSDTDKCSDNDDDAHDNSESKAKEGRVSDSETQGVDAMDPKL 900

BLAST of MC05g0295 vs. ExPASy TrEMBL
Match: A0A6J1D173 (uncharacterized protein LOC111016086 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016086 PE=4 SV=1)

HSP 1 Score: 1659 bits (4295), Expect = 0.0
Identity = 894/894 (100.00%), Postives = 894/894 (100.00%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60
           MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN
Sbjct: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60

Query: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120
           VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST
Sbjct: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120

Query: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180
           KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV
Sbjct: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180

Query: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240
           AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV
Sbjct: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240

Query: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300
           LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ
Sbjct: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300

Query: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360
           KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA
Sbjct: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360

Query: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420
           VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT
Sbjct: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420

Query: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL 480
           SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL
Sbjct: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL 480

Query: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540
           EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED
Sbjct: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540

Query: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600
           EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE
Sbjct: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600

Query: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660
           ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET
Sbjct: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660

Query: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720
           ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD
Sbjct: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720

Query: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780
           VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS
Sbjct: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780

Query: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840
           PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE
Sbjct: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840

Query: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 894
           NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG
Sbjct: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 894

BLAST of MC05g0295 vs. ExPASy TrEMBL
Match: A0A6J1D1B6 (kinesin-like protein KIF15 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016086 PE=4 SV=1)

HSP 1 Score: 1652 bits (4279), Expect = 0.0
Identity = 893/894 (99.89%), Postives = 893/894 (99.89%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60
           MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN
Sbjct: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTPREFILSVASKIASQPLQNYGSN 60

Query: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120
           VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST
Sbjct: 61  VWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADSRYQIESNSISANHCRIYRKRIST 120

Query: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180
           KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV
Sbjct: 121 KDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHGDIISLAAAPQHEVAFAFVYREVV 180

Query: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240
           AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV
Sbjct: 181 AFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPISLDDFRSLQRSNKELRRQLEDQV 240

Query: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300
           LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ
Sbjct: 241 LTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQ 300

Query: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360
           KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA
Sbjct: 301 KHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAA 360

Query: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420
           VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT
Sbjct: 361 VQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVT 420

Query: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLEL 480
           SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVK GAREEAWAKVSSLEL
Sbjct: 421 SDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQKELESVK-GAREEAWAKVSSLEL 480

Query: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540
           EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED
Sbjct: 481 EINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLED 540

Query: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600
           EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE
Sbjct: 541 EENYENTSFDFDLNVSLEAANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQAETSTEE 600

Query: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660
           ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET
Sbjct: 601 ASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLET 660

Query: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720
           ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD
Sbjct: 661 ESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVD 720

Query: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780
           VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS
Sbjct: 721 VDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRALQDSNS 780

Query: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840
           PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE
Sbjct: 781 PVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTE 840

Query: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 894
           NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG
Sbjct: 841 NCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQEDSLG 893

BLAST of MC05g0295 vs. ExPASy TrEMBL
Match: A0A6J1E9A4 (uncharacterized protein LOC111431865 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431865 PE=4 SV=1)

HSP 1 Score: 1296 bits (3354), Expect = 0.0
Identity = 724/917 (78.95%), Postives = 792/917 (86.37%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTP---------------------- 60
           MD DD   N ++ KS ALPK G+GSQSS SH+ PP TP                      
Sbjct: 1   MDGDDA--NPETPKSTALPKHGSGSQSSSSHIHPPHTPHSPPRPPPLNSVDETRSNKPLS 60

Query: 61  -REFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADS 120
            REF+LSVASKIASQPLQN+ SNVWGVLTAIS NARKRQQG+NILLT+DEH LGRV  DS
Sbjct: 61  PREFVLSVASKIASQPLQNFDSNVWGVLTAISGNARKRQQGINILLTDDEHCLGRVAPDS 120

Query: 121 RYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180
           RYQI+SNS+SA HCRIYRK I  +DACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG
Sbjct: 121 RYQIDSNSVSAKHCRIYRKSI--EDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180

Query: 181 DIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPI 240
           DIISLAAAPQHEVAFAFVYREV AFTSSS GG AKRKAE+ VSENKRLRGLGIGAPDGPI
Sbjct: 181 DIISLAAAPQHEVAFAFVYREVAAFTSSSGGGSAKRKAEDFVSENKRLRGLGIGAPDGPI 240

Query: 241 SLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIA 300
           SLDDFRSLQRSNKELR+QLED VL ID+L  ENRAS EHHE EVKK++ES+SK Y+D++ 
Sbjct: 241 SLDDFRSLQRSNKELRKQLEDHVLMIDSLRNENRASVEHHECEVKKLKESISKSYEDQLT 300

Query: 301 KYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKG 360
           K QQ  D +Q+ELGE  R++SEQKH IEDLQERLSATAQSC EANE+INSQKASLS+LK 
Sbjct: 301 KMQQLIDDEQKELGEVQRISSEQKHVIEDLQERLSATAQSCNEANEIINSQKASLSELKV 360

Query: 361 QIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESE 420
           QIDEERDQRREEREKAAADLKAAV+KAHA AQDE+KR+SDAAS REREQQEVINKLQE++
Sbjct: 361 QIDEERDQRREEREKAAADLKAAVEKAHAVAQDELKRISDAASKREREQQEVINKLQEAD 420

Query: 421 KERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQ 480
           KERC  VETLRSKLEETRQKLV SDNKVRQLESQLGE+QLSC NERKKVEELE G+KELQ
Sbjct: 421 KERCFQVETLRSKLEETRQKLVMSDNKVRQLESQLGEEQLSCTNERKKVEELERGIKELQ 480

Query: 481 KELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540
           KELES KQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT
Sbjct: 481 KELESEKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540

Query: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVSLEAANGILLGERVQENYCNKS 600
           EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNV  + ANGILLG+ ++++YCNKS
Sbjct: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVPSQDANGILLGDNIRKDYCNKS 600

Query: 601 AKTSSAMSAQRFDPIQAETSTEEASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSD 660
            KTSSA SAQRF+P+QAETST+EASTEKND + RSQDC+NTQE EFTSADAGVKGGFGSD
Sbjct: 601 DKTSSATSAQRFEPVQAETSTDEASTEKNDCDFRSQDCQNTQEAEFTSADAGVKGGFGSD 660

Query: 661 IDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNE 720
           IDGVGTAPVLEGD VGTER+LETESPGVDG++N D NKG+ LAGET+C DDEG AGE +E
Sbjct: 661 IDGVGTAPVLEGDMVGTERILETESPGVDGDQNMDLNKGMILAGETICFDDEGCAGEMDE 720

Query: 721 QGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHG 780
           Q KM+ QEA CHSQTNQICDAV+  A EDTEA GTVRTADLLASEVAGSWA STAPSVHG
Sbjct: 721 QAKMVRQEAYCHSQTNQICDAVE--AVEDTEACGTVRTADLLASEVAGSWASSTAPSVHG 780

Query: 781 ENESPRSRGNEEEGGRALQDSNSPVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKH 840
           ENES RSRGNE  G  A+ DSNSP  G QS      ATRRN E+Q +SEMI+IVAPESK 
Sbjct: 781 ENESQRSRGNEGGGDGAIHDSNSP--GIQSTLFKPVATRRNSEYQTVSEMIRIVAPESKQ 840

Query: 841 FFGSREDGCERGGEESASSSDTENCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPEL 894
           FF SREDG E G ++S S SDT+ C+DNDDDA +N+E +AKEGRVSDSETQGVD +DP+L
Sbjct: 841 FFHSREDGRE-GEQDSTSGSDTDKCSDNDDDAHDNSESKAKEGRVSDSETQGVDAMDPKL 900

BLAST of MC05g0295 vs. ExPASy TrEMBL
Match: A0A6J1E8N3 (uncharacterized protein LOC111431865 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431865 PE=4 SV=1)

HSP 1 Score: 1290 bits (3338), Expect = 0.0
Identity = 723/917 (78.84%), Postives = 791/917 (86.26%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHLPPPPTP---------------------- 60
           MD DD   N ++ KS ALPK G+GSQSS SH+ PP TP                      
Sbjct: 1   MDGDDA--NPETPKSTALPKHGSGSQSSSSHIHPPHTPHSPPRPPPLNSVDETRSNKPLS 60

Query: 61  -REFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADS 120
            REF+LSVASKIASQPLQN+ SNVWGVLTAIS NARKRQQG+NILLT+DEH LGRV  DS
Sbjct: 61  PREFVLSVASKIASQPLQNFDSNVWGVLTAISGNARKRQQGINILLTDDEHCLGRVAPDS 120

Query: 121 RYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180
           RYQI+SNS+SA HCRIYRK I  +DACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG
Sbjct: 121 RYQIDSNSVSAKHCRIYRKSI--EDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180

Query: 181 DIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPI 240
           DIISLAAAPQHEVAFAFVYREV AFTSSS GG AKRKAE+ VSENKRLRGLGIGAPDGPI
Sbjct: 181 DIISLAAAPQHEVAFAFVYREVAAFTSSSGGGSAKRKAEDFVSENKRLRGLGIGAPDGPI 240

Query: 241 SLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIA 300
           SLDDFRSLQRSNKELR+QLED VL ID+L  ENRAS EHHE EVKK++ES+SK Y+D++ 
Sbjct: 241 SLDDFRSLQRSNKELRKQLEDHVLMIDSLRNENRASVEHHECEVKKLKESISKSYEDQLT 300

Query: 301 KYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKG 360
           K QQ  D +Q+ELGE  R++SEQKH IEDLQERLSATAQSC EANE+INSQKASLS+LK 
Sbjct: 301 KMQQLIDDEQKELGEVQRISSEQKHVIEDLQERLSATAQSCNEANEIINSQKASLSELKV 360

Query: 361 QIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESE 420
           QIDEERDQRREEREKAAADLKAAV+KAHA AQDE+KR+SDAAS REREQQEVINKLQE++
Sbjct: 361 QIDEERDQRREEREKAAADLKAAVEKAHAVAQDELKRISDAASKREREQQEVINKLQEAD 420

Query: 421 KERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQ 480
           KERC  VETLRSKLEETRQKLV SDNKVRQLESQLGE+QLSC NERKKVEELE G+KELQ
Sbjct: 421 KERCFQVETLRSKLEETRQKLVMSDNKVRQLESQLGEEQLSCTNERKKVEELERGIKELQ 480

Query: 481 KELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540
           KELES K GAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT
Sbjct: 481 KELESEK-GAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540

Query: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVSLEAANGILLGERVQENYCNKS 600
           EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNV  + ANGILLG+ ++++YCNKS
Sbjct: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVPSQDANGILLGDNIRKDYCNKS 600

Query: 601 AKTSSAMSAQRFDPIQAETSTEEASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSD 660
            KTSSA SAQRF+P+QAETST+EASTEKND + RSQDC+NTQE EFTSADAGVKGGFGSD
Sbjct: 601 DKTSSATSAQRFEPVQAETSTDEASTEKNDCDFRSQDCQNTQEAEFTSADAGVKGGFGSD 660

Query: 661 IDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNE 720
           IDGVGTAPVLEGD VGTER+LETESPGVDG++N D NKG+ LAGET+C DDEG AGE +E
Sbjct: 661 IDGVGTAPVLEGDMVGTERILETESPGVDGDQNMDLNKGMILAGETICFDDEGCAGEMDE 720

Query: 721 QGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHG 780
           Q KM+ QEA CHSQTNQICDAV+  A EDTEA GTVRTADLLASEVAGSWA STAPSVHG
Sbjct: 721 QAKMVRQEAYCHSQTNQICDAVE--AVEDTEACGTVRTADLLASEVAGSWASSTAPSVHG 780

Query: 781 ENESPRSRGNEEEGGRALQDSNSPVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKH 840
           ENES RSRGNE  G  A+ DSNSP  G QS      ATRRN E+Q +SEMI+IVAPESK 
Sbjct: 781 ENESQRSRGNEGGGDGAIHDSNSP--GIQSTLFKPVATRRNSEYQTVSEMIRIVAPESKQ 840

Query: 841 FFGSREDGCERGGEESASSSDTENCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPEL 894
           FF SREDG E G ++S S SDT+ C+DNDDDA +N+E +AKEGRVSDSETQGVD +DP+L
Sbjct: 841 FFHSREDGRE-GEQDSTSGSDTDKCSDNDDDAHDNSESKAKEGRVSDSETQGVDAMDPKL 900

BLAST of MC05g0295 vs. ExPASy TrEMBL
Match: A0A6J1IQS8 (uncharacterized protein LOC111479660 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479660 PE=4 SV=1)

HSP 1 Score: 1288 bits (3333), Expect = 0.0
Identity = 719/917 (78.41%), Postives = 792/917 (86.37%), Query Frame = 0

Query: 1   MDADDHPENSQSRKSNALPKDGNGSQSSFSHL----------PPPP-------------T 60
           MD +D   N ++ KS ALPK G+GSQSS SH+          PPPP             +
Sbjct: 1   MDGEDA--NPEAPKSTALPKHGSGSQSSSSHIHPPHPPHSPPPPPPLNSVDETRSNKPLS 60

Query: 61  PREFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRVVADS 120
           PREF+LSVASKIASQPLQN+ SNVWGVLTAIS NARKRQQG+NILLT+DEH LGRV  DS
Sbjct: 61  PREFVLSVASKIASQPLQNFDSNVWGVLTAISGNARKRQQGINILLTDDEHCLGRVAPDS 120

Query: 121 RYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180
           RYQI+SNS+SA HCRIYRK I  +DACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG
Sbjct: 121 RYQIDSNSVSAKHCRIYRKSI--EDACCPSVFLKDTSTNGTYLNWERLKKNSQEAKICHG 180

Query: 181 DIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAPDGPI 240
           DIISLAAAPQHEVAFAFVYREV AFTS+S GG AKRKAE+ VSENKRLRGLGIGAPDGPI
Sbjct: 181 DIISLAAAPQHEVAFAFVYREVAAFTSASGGGSAKRKAEDFVSENKRLRGLGIGAPDGPI 240

Query: 241 SLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHELEVKKVRESVSKLYDDEIA 300
           SLDDFRSLQRSNKELR+QLEDQ+L ID+L  ENRAS EHHE EVKK++ES+SK Y+D++ 
Sbjct: 241 SLDDFRSLQRSNKELRKQLEDQMLMIDSLRNENRASVEHHECEVKKLKESISKSYEDQLT 300

Query: 301 KYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLSKLKG 360
           K QQ  D +Q+ELGE  R++SEQKH IEDLQERLSATAQSC EANE+INSQKASLS+LK 
Sbjct: 301 KMQQLIDDEQKELGEVQRISSEQKHVIEDLQERLSATAQSCNEANEIINSQKASLSELKV 360

Query: 361 QIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKLQESE 420
           QIDEERDQRREEREKAAADLKAAV+KAHA AQDE+KR+SDAAS REREQQEVINKLQE+E
Sbjct: 361 QIDEERDQRREEREKAAADLKAAVEKAHAVAQDELKRISDAASRREREQQEVINKLQEAE 420

Query: 421 KERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGVKELQ 480
           KERC  VETLRSKLEETRQKLV SDNKVRQLESQLGE+QLSC NERKKVEEL+ G+KELQ
Sbjct: 421 KERCFQVETLRSKLEETRQKLVMSDNKVRQLESQLGEEQLSCTNERKKVEELDRGIKELQ 480

Query: 481 KELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540
           KELE+ KQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT
Sbjct: 481 KELENEKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAFYSTT 540

Query: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVSLEAANGILLGERVQENYCNKS 600
           EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNV  + ANGIL G+ ++++YCNKS
Sbjct: 541 EEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLNVPSQDANGILFGDNIRKDYCNKS 600

Query: 601 AKTSSAMSAQRFDPIQAETSTEEASTEKNDGNIRSQDCENTQEVEFTSADAGVKGGFGSD 660
            KTSSAMSAQRF+P+QAETST+EASTEKND + RSQDC+NTQE EFTSADAGVKGGFGSD
Sbjct: 601 DKTSSAMSAQRFEPVQAETSTDEASTEKNDCDFRSQDCQNTQEAEFTSADAGVKGGFGSD 660

Query: 661 IDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKGIALAGETMCLDDEGRAGETNE 720
           IDGVGTAPVLEGD VGTER+LETESPGVDG+RN D NKG+ LAGET+C DDEG AGE +E
Sbjct: 661 IDGVGTAPVLEGDMVGTERILETESPGVDGDRNMDLNKGMILAGETICFDDEGCAGEMDE 720

Query: 721 QGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTADLLASEVAGSWACSTAPSVHG 780
           Q KM+ +EA CHSQTNQICDAVD  A EDTEA GTVRTADLLASEVAGSWA STAPSVH 
Sbjct: 721 QAKMVRREAYCHSQTNQICDAVD--AVEDTEACGTVRTADLLASEVAGSWASSTAPSVHS 780

Query: 781 ENESPRSRGNEEEGGRALQDSNSPVAGSQSAPSTAAATRRNFEHQALSEMIQIVAPESKH 840
           ENES +SRGNE  G  AL DSNSP  G +S      ATRRN E+Q +SEMI+IVAPESK 
Sbjct: 781 ENESQKSRGNEGGGDGALHDSNSP--GIRSTLFKPVATRRNSEYQTVSEMIRIVAPESKQ 840

Query: 841 FFGSREDGCERGGEESASSSDTENCTDNDDDAINNNEKEAKEGRVSDSETQGVDLVDPEL 894
           FF SREDG E G + S S SDT+ C+DNDDDA +NNE +A++G VSDSETQGVD +DP+L
Sbjct: 841 FFRSREDGRE-GEQGSTSGSDTDKCSDNDDDAHDNNESKAQKGTVSDSETQGVDAIDPKL 900

BLAST of MC05g0295 vs. TAIR 10
Match: AT2G45460.2 (SMAD/FHA domain-containing protein )

HSP 1 Score: 750.0 bits (1935), Expect = 2.2e-216
Identity = 460/869 (52.93%), Postives = 604/869 (69.51%), Query Frame = 0

Query: 34  PPPTPREFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRV 93
           P    ++FI+S A+ IASQPLQNY SNVWGVLTAIS NARKR+QG+NILLT DEH LGR+
Sbjct: 43  PSLNSKDFIVSAAANIASQPLQNYDSNVWGVLTAISSNARKRRQGINILLTSDEHCLGRL 102

Query: 94  VADSRYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAK 153
              + YQ+ESN+IS NHC+++RK ++  D    +VF+ DTSTNGT+LNWERL KN  E +
Sbjct: 103 PCHASYQVESNAISGNHCKVFRKPVTGGDGDDVTVFMVDTSTNGTFLNWERLTKNGPEVR 162

Query: 154 ICHGDIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAP 213
           + HGDIISLA  P+HE AFAFVYREV+    +      KRKAE+   E KR +G+GI  P
Sbjct: 163 VQHGDIISLAVPPEHEKAFAFVYREVLGNNPALSCMNRKRKAEDTTCEIKRQKGIGISGP 222

Query: 214 DGPISLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHELEVKKVRESVSKLYD 273
           +GPISLDDF+SLQRSN ELR+QLE QVLTID L  E+R+  EHHE E+K+++ES +K + 
Sbjct: 223 NGPISLDDFKSLQRSNTELRKQLEAQVLTIDTLRNESRSIVEHHESEIKQIKESTAKSFH 282

Query: 274 DEIAKYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCKEANEVINSQKASLS 333
           +E+ + +   D  Q+EL +  +L++EQK+SI++L ER+SA+ Q+  EANEVI SQKAS++
Sbjct: 283 NELIELRDQLDTKQKELAQVNKLSAEQKNSIDELGERVSASLQTLSEANEVIQSQKASIA 342

Query: 334 KLKGQIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAASMREREQQEVINKL 393
           +LK  +DEER+QRREERE A A+LKAA+ +   EAQ+E+KR SDAA   EREQQEVINK+
Sbjct: 343 ELKTGLDEERNQRREERETAIAELKAAIHRCQIEAQEELKRFSDAAMRHEREQQEVINKM 402

Query: 394 QESEKERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSCANERKKVEELEGGV 453
           +ESEKE+ + VETL SKLE+TRQ+LV S+N+ R LE+Q+ E+QL+ A+ +KK+EEL+  V
Sbjct: 403 KESEKEKSMQVETLMSKLEDTRQRLVCSENRNRLLEAQVSEEQLAFADAQKKLEELDLQV 462

Query: 454 KELQKELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGARERIMLRETQLRAF 513
           K LQK+L+S KQ AREEAWAKVS+LELEI+AA+RDLD ER+R +GARERIMLRETQ+RAF
Sbjct: 463 KRLQKDLDSEKQAAREEAWAKVSALELEISAAVRDLDVERQRHRGARERIMLRETQMRAF 522

Query: 514 YSTTEEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLN-VSLEAANGILLGERVQEN 573
           YSTTEEISALFAKQQEQLK MQRTLEDE+N +NTS D DLN ++         G++   +
Sbjct: 523 YSTTEEISALFAKQQEQLKTMQRTLEDEDNCDNTSLDIDLNPINRSPNRANTQGDKRATS 582

Query: 574 YCNKSAKTSSAMSAQRFDPIQ-AETSTEEA-STEKNDGNIRSQDCENTQEVEFTSADAGV 633
           + N +A+ SS+ S QR    +  +TS E+A +T+K+D  I SQ+ +NTQE E+ S+D   
Sbjct: 583 HLNFAARASSSTSGQRSTRNEVVDTSCEDADATQKHDCEIMSQEGQNTQEAEYPSSDKVA 642

Query: 634 KGGFGSDIDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKGIALAGETMCLDDEG 693
           KGGFGSDI+G+GTAP    D VGTE+V ET+SPG D ERN    K I LAG+TM +D E 
Sbjct: 643 KGGFGSDIEGIGTAPTSGTDPVGTEQVNETQSPGNDYERNDHLRKSIILAGDTMQIDCE- 702

Query: 694 RAGETNEQGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTADLLASEVAGSWACS 753
                      +H+          + +  D    +D E  GT+ T+DLLASEVAGSWA S
Sbjct: 703 ---------TQVHESVQIEGAVLLLRNPNDRRDTQDIEGVGTIGTSDLLASEVAGSWANS 762

Query: 754 TAPSVHGENESPRSRGNEEEGGRAL------QDSNSPVAGSQ---SAPSTAAATRRNFEH 813
           T PSVHGENE+ RSR +EE   + +      QDS   +  SQ   ++P      + + E 
Sbjct: 763 TNPSVHGENETERSREDEESQTQKIKEVTIVQDSAGQIGESQTKPTSPGVLVTNKDDAER 822

Query: 814 QALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTENCTDNDDDAINNNEKEAKEGR 873
             ++E + I   + K   G+R D            S+TE+C+D+DDD    +EKE K   
Sbjct: 823 GVINEPVGI-TDQGKIKHGTRSD------------SETESCSDSDDD----HEKE-KHNP 882

Query: 874 VSDSETQGVDLVDPELDDPMDQDDEATQE 891
           VSDS+T+G D+ D +     D D E + E
Sbjct: 883 VSDSDTEGSDMNDDKGSLSSDPDTERSHE 883

BLAST of MC05g0295 vs. TAIR 10
Match: AT2G45460.3 (SMAD/FHA domain-containing protein )

HSP 1 Score: 740.3 bits (1910), Expect = 1.8e-213
Identity = 460/883 (52.10%), Postives = 604/883 (68.40%), Query Frame = 0

Query: 34  PPPTPREFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRV 93
           P    ++FI+S A+ IASQPLQNY SNVWGVLTAIS NARKR+QG+NILLT DEH LGR+
Sbjct: 43  PSLNSKDFIVSAAANIASQPLQNYDSNVWGVLTAISSNARKRRQGINILLTSDEHCLGRL 102

Query: 94  VADSRYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAK 153
              + YQ+ESN+IS NHC+++RK ++  D    +VF+ DTSTNGT+LNWERL KN  E +
Sbjct: 103 PCHASYQVESNAISGNHCKVFRKPVTGGDGDDVTVFMVDTSTNGTFLNWERLTKNGPEVR 162

Query: 154 ICHGDIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAP 213
           + HGDIISLA  P+HE AFAFVYREV+    +      KRKAE+   E KR +G+GI  P
Sbjct: 163 VQHGDIISLAVPPEHEKAFAFVYREVLGNNPALSCMNRKRKAEDTTCEIKRQKGIGISGP 222

Query: 214 DGPISLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHE--------------L 273
           +GPISLDDF+SLQRSN ELR+QLE QVLTID L  E+R+  EHHE               
Sbjct: 223 NGPISLDDFKSLQRSNTELRKQLEAQVLTIDTLRNESRSIVEHHESDYLSISTEISLHLQ 282

Query: 274 EVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCK 333
           E+K+++ES +K + +E+ + +   D  Q+EL +  +L++EQK+SI++L ER+SA+ Q+  
Sbjct: 283 EIKQIKESTAKSFHNELIELRDQLDTKQKELAQVNKLSAEQKNSIDELGERVSASLQTLS 342

Query: 334 EANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAA 393
           EANEVI SQKAS+++LK  +DEER+QRREERE A A+LKAA+ +   EAQ+E+KR SDAA
Sbjct: 343 EANEVIQSQKASIAELKTGLDEERNQRREERETAIAELKAAIHRCQIEAQEELKRFSDAA 402

Query: 394 SMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSC 453
              EREQQEVINK++ESEKE+ + VETL SKLE+TRQ+LV S+N+ R LE+Q+ E+QL+ 
Sbjct: 403 MRHEREQQEVINKMKESEKEKSMQVETLMSKLEDTRQRLVCSENRNRLLEAQVSEEQLAF 462

Query: 454 ANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGA 513
           A+ +KK+EEL+  VK LQK+L+S KQ AREEAWAKVS+LELEI+AA+RDLD ER+R +GA
Sbjct: 463 ADAQKKLEELDLQVKRLQKDLDSEKQAAREEAWAKVSALELEISAAVRDLDVERQRHRGA 522

Query: 514 RERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLN-VSLE 573
           RERIMLRETQ+RAFYSTTEEISALFAKQQEQLK MQRTLEDE+N +NTS D DLN ++  
Sbjct: 523 RERIMLRETQMRAFYSTTEEISALFAKQQEQLKTMQRTLEDEDNCDNTSLDIDLNPINRS 582

Query: 574 AANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQ-AETSTEEA-STEKNDGNIRSQDCE 633
                  G++   ++ N +A+ SS+ S QR    +  +TS E+A +T+K+D  I SQ+ +
Sbjct: 583 PNRANTQGDKRATSHLNFAARASSSTSGQRSTRNEVVDTSCEDADATQKHDCEIMSQEGQ 642

Query: 634 NTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKG 693
           NTQE E+ S+D   KGGFGSDI+G+GTAP    D VGTE+V ET+SPG D ERN    K 
Sbjct: 643 NTQEAEYPSSDKVAKGGFGSDIEGIGTAPTSGTDPVGTEQVNETQSPGNDYERNDHLRKS 702

Query: 694 IALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTA 753
           I LAG+TM +D E            +H+          + +  D    +D E  GT+ T+
Sbjct: 703 IILAGDTMQIDCE----------TQVHESVQIEGAVLLLRNPNDRRDTQDIEGVGTIGTS 762

Query: 754 DLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRAL------QDSNSPVAGSQ---S 813
           DLLASEVAGSWA ST PSVHGENE+ RSR +EE   + +      QDS   +  SQ   +
Sbjct: 763 DLLASEVAGSWANSTNPSVHGENETERSREDEESQTQKIKEVTIVQDSAGQIGESQTKPT 822

Query: 814 APSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTENCTDNDD 873
           +P      + + E   ++E + I   + K   G+R D            S+TE+C+D+DD
Sbjct: 823 SPGVLVTNKDDAERGVINEPVGI-TDQGKIKHGTRSD------------SETESCSDSDD 882

Query: 874 DAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQE 891
           D    +EKE K   VSDS+T+G D+ D +     D D E + E
Sbjct: 883 D----HEKE-KHNPVSDSDTEGSDMNDDKGSLSSDPDTERSHE 897

BLAST of MC05g0295 vs. TAIR 10
Match: AT2G45460.1 (SMAD/FHA domain-containing protein )

HSP 1 Score: 734.2 bits (1894), Expect = 1.3e-211
Identity = 459/883 (51.98%), Postives = 603/883 (68.29%), Query Frame = 0

Query: 34  PPPTPREFILSVASKIASQPLQNYGSNVWGVLTAISDNARKRQQGMNILLTEDEHLLGRV 93
           P    ++FI+S A+ IASQPLQNY SNVWGVLTAIS NARKR+QG+NILLT DEH LGR+
Sbjct: 43  PSLNSKDFIVSAAANIASQPLQNYDSNVWGVLTAISSNARKRRQGINILLTSDEHCLGRL 102

Query: 94  VADSRYQIESNSISANHCRIYRKRISTKDACCPSVFLKDTSTNGTYLNWERLKKNSQEAK 153
              + YQ+ESN+IS NHC+++RK ++  D    +VF+ DTSTNGT+LNWERL KN  E +
Sbjct: 103 PCHASYQVESNAISGNHCKVFRKPVTGGDGDDVTVFMVDTSTNGTFLNWERLTKNGPEVR 162

Query: 154 ICHGDIISLAAAPQHEVAFAFVYREVVAFTSSSDGGPAKRKAENVVSENKRLRGLGIGAP 213
           + HGDIISLA  P+HE AFAFVYREV+    +      KRKAE+   E KR +G+GI  P
Sbjct: 163 VQHGDIISLAVPPEHEKAFAFVYREVLGNNPALSCMNRKRKAEDTTCEIKRQKGIGISGP 222

Query: 214 DGPISLDDFRSLQRSNKELRRQLEDQVLTIDALHKENRASKEHHE--------------L 273
           +GPISLDDF+SLQRSN ELR+QLE QVLTID L  E+R+  EHHE               
Sbjct: 223 NGPISLDDFKSLQRSNTELRKQLEAQVLTIDTLRNESRSIVEHHESDYLSISTEISLHLQ 282

Query: 274 EVKKVRESVSKLYDDEIAKYQQSTDRDQRELGEAARLASEQKHSIEDLQERLSATAQSCK 333
           E+K+++ES +K + +E+ + +   D  Q+EL +  +L++EQK+SI++L ER+SA+ Q+  
Sbjct: 283 EIKQIKESTAKSFHNELIELRDQLDTKQKELAQVNKLSAEQKNSIDELGERVSASLQTLS 342

Query: 334 EANEVINSQKASLSKLKGQIDEERDQRREEREKAAADLKAAVQKAHAEAQDEVKRLSDAA 393
           EANEVI SQKAS+++LK  +DEER+QRREERE A A+LKAA+ +   EAQ+E+KR SDAA
Sbjct: 343 EANEVIQSQKASIAELKTGLDEERNQRREERETAIAELKAAIHRCQIEAQEELKRFSDAA 402

Query: 394 SMREREQQEVINKLQESEKERCLLVETLRSKLEETRQKLVTSDNKVRQLESQLGEQQLSC 453
              EREQQEVINK++ESEKE+ + VETL SKLE+TRQ+LV S+N+ R LE+Q+ E+QL+ 
Sbjct: 403 MRHEREQQEVINKMKESEKEKSMQVETLMSKLEDTRQRLVCSENRNRLLEAQVSEEQLAF 462

Query: 454 ANERKKVEELEGGVKELQKELESVKQGAREEAWAKVSSLELEINAAIRDLDFERRRLKGA 513
           A+ +KK+EEL+  VK LQK+L+S K  AREEAWAKVS+LELEI+AA+RDLD ER+R +GA
Sbjct: 463 ADAQKKLEELDLQVKRLQKDLDSEK-AAREEAWAKVSALELEISAAVRDLDVERQRHRGA 522

Query: 514 RERIMLRETQLRAFYSTTEEISALFAKQQEQLKAMQRTLEDEENYENTSFDFDLN-VSLE 573
           RERIMLRETQ+RAFYSTTEEISALFAKQQEQLK MQRTLEDE+N +NTS D DLN ++  
Sbjct: 523 RERIMLRETQMRAFYSTTEEISALFAKQQEQLKTMQRTLEDEDNCDNTSLDIDLNPINRS 582

Query: 574 AANGILLGERVQENYCNKSAKTSSAMSAQRFDPIQ-AETSTEEA-STEKNDGNIRSQDCE 633
                  G++   ++ N +A+ SS+ S QR    +  +TS E+A +T+K+D  I SQ+ +
Sbjct: 583 PNRANTQGDKRATSHLNFAARASSSTSGQRSTRNEVVDTSCEDADATQKHDCEIMSQEGQ 642

Query: 634 NTQEVEFTSADAGVKGGFGSDIDGVGTAPVLEGDAVGTERVLETESPGVDGERNTDSNKG 693
           NTQE E+ S+D   KGGFGSDI+G+GTAP    D VGTE+V ET+SPG D ERN    K 
Sbjct: 643 NTQEAEYPSSDKVAKGGFGSDIEGIGTAPTSGTDPVGTEQVNETQSPGNDYERNDHLRKS 702

Query: 694 IALAGETMCLDDEGRAGETNEQGKMIHQEACCHSQTNQICDAVDVDAREDTEAGGTVRTA 753
           I LAG+TM +D E            +H+          + +  D    +D E  GT+ T+
Sbjct: 703 IILAGDTMQIDCE----------TQVHESVQIEGAVLLLRNPNDRRDTQDIEGVGTIGTS 762

Query: 754 DLLASEVAGSWACSTAPSVHGENESPRSRGNEEEGGRAL------QDSNSPVAGSQ---S 813
           DLLASEVAGSWA ST PSVHGENE+ RSR +EE   + +      QDS   +  SQ   +
Sbjct: 763 DLLASEVAGSWANSTNPSVHGENETERSREDEESQTQKIKEVTIVQDSAGQIGESQTKPT 822

Query: 814 APSTAAATRRNFEHQALSEMIQIVAPESKHFFGSREDGCERGGEESASSSDTENCTDNDD 873
           +P      + + E   ++E + I   + K   G+R D            S+TE+C+D+DD
Sbjct: 823 SPGVLVTNKDDAERGVINEPVGI-TDQGKIKHGTRSD------------SETESCSDSDD 882

Query: 874 DAINNNEKEAKEGRVSDSETQGVDLVDPELDDPMDQDDEATQE 891
           D    +EKE K   VSDS+T+G D+ D +     D D E + E
Sbjct: 883 D----HEKE-KHNPVSDSDTEGSDMNDDKGSLSSDPDTERSHE 896