MC05g0222 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0222
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	10 kDa chaperonin
Location	MC05: 1586065 .. 1588848 (-)
RNA-Seq Expression	MC05g0222
Synteny	MC05g0222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCGCGCACCCCGCTCTACTCCGTAGATTTTTGCTTTCTCGACTCATTTCCATGGCGATGGCGTCTTCGTTCGTTACTGTGCCGAAGCCCTTTGTCGACAGGGCTAATTCACCTTCTGTTTCTTCATGGCGGCAAAAAGCTGGTACACTGAAAATTCCGTTGATAGATTGTTGCATTTTCTTTTTCTCGATCAATTTCTGTCTCATTCCGTCTTCTTGGATAGGATTGCAGAGAAGCTCTTTGAAAGTCAGCGCAGTTTCTAAGAAATATAAGCCTGCGAAGGTTTTCCACTTTTTTCCCCTAAAATATCTTATGCGATTTTAGATTTTTTCTGTCTTATGTGTTATTGAACTTGTGGTCTTGATTTCAATGCTTATTTTTGGCGTTTGATGATGCTTGTGGATTTCAGGTGGTTCCTCAAGCTGACAGAGTCCTCGTTCGTCTTGAGGAGCTTGCTGAGGTAAGCTCAAGCTTAACACGAATGGCGAATGATTTTGTTGAATTCTATAACTCGTAGAGCTTTTTGGATGTGTTGAGAACTGTAAAATGTTCCTAAATTTGAGATGTCTTATTATGCAGAAATCGGCTGGTGGAGTTTTGCTACCCAAATCAGCTGTCAAATTTGAGCGACATCTTGTGGGAGAGGTGAGGAAATCCTTCTGTTGCTGTTGCCATTTTCTCTATATTTTTTCCTGTTAGGTAATGTATTTGACAATCAATATTATATTTCTTTGTATCAGATTATCTCCATTGGAACAGAAGTTGGAGGAAATGATATTGCACCTGGAAAGAAGGTAATTGAGGATTCTTCTTTATTAAAATTTCAGGCCGTAATTAGTGTTACCCAAATTTGGTTTATCTGAAGAAAGTAAGCGTGAGTTTGTAGTTTTTTTAAGGGAAGCTTTTTAATAAGCACATCTGGAAAGTACTTTAAAATGTTTTTACTGTATTTCAAATTTTAAGAACTCTTTTGGCATGGAACCAAACATATGAGAAATTTGACAAGTGCTTTTAATTAGTGAAAAGCATTTCCTATTGTTTGAAAACCATGCCAAATCCACCCTTATTATTATGCAAACTTGTGGGACTTGTTTCTTTTCTAAGAGGAGCAATTATTTATTACCTTGCAGGTTCTTATATCAGACATCAATGCTTATGAGGTAAGTCGTTTGGTTTGGTGGTGTACATTATTGGCTTGAATATTCTCTCAATTATATTTGGTTCAATACTCCTTCAAAGTATTGGTTACAAAATTATCCATAGCCCTGGGACAGTGTCTCGATCTCCTACGTGTGTGCTATCATCGAGAACGGGGGATTGTAGAAGACTTAGGTTAGTTTATGCGAGTCTTCCTAGAATTAGTTTGGGTTTGAACCAAATTAACTCAAACTTATTATTAATTAAAAATATCTTACTTATGATGTCATTACCTGTGCATACATTTTAATTTATTTTCATTTTATTCAATAATTCTCTTTTGATCTTTCACCCAACAACTTTAAGAAAATGGTGTTGTAGGTCTCTTGGAGTATAGTTTTTCATGAAATAAAATTTGACAATAGACTACTAATTTAAACTCAACGTTTGAAAATCAGTAATCGGATATCTAAGGGACTGACATTTTCACTATTTCTCCTTACAAAATTTTGAACAAATGACCCGGAAAATAGCACGTTTTTTATTCTTTGAAAGTCCTAAAAAGATGGTCGCAATTTCTAATGCTTAACATTTTGCCTATGAGCTAGTTGTCTTGGGATGTTAATTATTTCACCAAATACTGCCATTCTTACTGTAAAGTTAAACTGATTGATGCAGAATTCCCATTAGTGACTCATTAGACAAAAAAAGTAGCAGGACTCTAATTCCAGGGCCATAAGTAGTAAATATGTTTTTAGGGGAAATGTTTGTTAAGCTGCCTAATCTTTTGGCAATGCATCCTTCGAACCTTTATCTTCTAACTTGTATCATGTATGTTAGCTAATCGCTATCACTCATGTCATGAAGTAACTCATGTTCATTTGTTTCTTTCTATAGATTTTTTTCTTCGGTTATTGAATCGCAGGATTAAATGATGGCAGATACTTAATCCTGCAAGTTTTTCAGTGTTACATATTGCAAGGTCTGAGTGATTATCACATGTGCCTAGTCAAGATGCGTGTACCTAGATACGCATAAAAAGAAAAACTGTTTGCAATTAGAGTGATATAACTAATGTACGTTGTTACAATGCTGTAAGTCATGATCCTGAATAGCAAAGTGATCGGGATCGGGATCGGGTTTGCCCATGCATGATGGAATCATTGCTTTGTTTAATTTGTTGGTGAGCATTTGACAAACAAATAAACTTCTAAGCACAGCCAAGATTTGGATGATCCATCCCTTGAAACATATTTGTGTGTTGTAATAGATTCATTGTGAATGTTGGTGGTTTCTCAGGTGGATTTGGGCACAGATGCTAAGCACTGCTTCTGTAAAGCTGGTGATTTGTTAGCTGTGGTTGAGTAGAGAAGCTTTACAATTTCTCCAACGCTACAAAATTCAGATCAACATCACTTATTCCACTCAGATTAGTTCTCTCTTTCCTCATTGATTTTCAATTGCTTTTTTACTTTTGAAGTATATCATGTCTTTATACTTTTATTTAAGAATTACCCATCATTGTAAAGTTTCCTCAAAAAAAAAAAGAAAAAAGTGAGACTTGTAAAGAACTCATTAGGAAGAATAGCTTAAATTAAAAAGAATTAGTCAACCTAAATAGTAAACTAAAGGAAAATAGGTATGCAA

mRNA sequence

CCCGCGCACCCCGCTCTACTCCGTAGATTTTTGCTTTCTCGACTCATTTCCATGGCGATGGCGTCTTCGTTCGTTACTGTGCCGAAGCCCTTTGTCGACAGGGCTAATTCACCTTCTGTTTCTTCATGGCGGCAAAAAGCTGGATTGCAGAGAAGCTCTTTGAAAGTCAGCGCAGTTTCTAAGAAATATAAGCCTGCGAAGGTGGTTCCTCAAGCTGACAGAGTCCTCGTTCGTCTTGAGGAGCTTGCTGAGAAATCGGCTGGTGGAGTTTTGCTACCCAAATCAGCTGTCAAATTTGAGCGACATCTTGTGGGAGAGATTATCTCCATTGGAACAGAAGTTGGAGGAAATGATATTGCACCTGGAAAGAAGGTTCTTATATCAGACATCAATGCTTATGAGGTGGATTTGGGCACAGATGCTAAGCACTGCTTCTGTAAAGCTGGTGATTTGTTAGCTGTGGTTGAGTAGAGAAGCTTTACAATTTCTCCAACGCTACAAAATTCAGATCAACATCACTTATTCCACTCAGATTAGTTCTCTCTTTCCTCATTGATTTTCAATTGCTTTTTTACTTTTGAAGTATATCATGTCTTTATACTTTTATTTAAGAATTACCCATCATTGTAAAGTTTCCTCAAAAAAAAAAAGAAAAAAGTGAGACTTGTAAAGAACTCATTAGGAAGAATAGCTTAAATTAAAAAGAATTAGTCAACCTAAATAGTAAACTAAAGGAAAATAGGTATGCAA

Coding sequence (CDS)

ATGGCGATGGCGTCTTCGTTCGTTACTGTGCCGAAGCCCTTTGTCGACAGGGCTAATTCACCTTCTGTTTCTTCATGGCGGCAAAAAGCTGGATTGCAGAGAAGCTCTTTGAAAGTCAGCGCAGTTTCTAAGAAATATAAGCCTGCGAAGGTGGTTCCTCAAGCTGACAGAGTCCTCGTTCGTCTTGAGGAGCTTGCTGAGAAATCGGCTGGTGGAGTTTTGCTACCCAAATCAGCTGTCAAATTTGAGCGACATCTTGTGGGAGAGATTATCTCCATTGGAACAGAAGTTGGAGGAAATGATATTGCACCTGGAAAGAAGGTTCTTATATCAGACATCAATGCTTATGAGGTGGATTTGGGCACAGATGCTAAGCACTGCTTCTGTAAAGCTGGTGATTTGTTAGCTGTGGTTGAGTAG

Protein sequence

MAMASSFVTVPKPFVDRANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVLVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVDLGTDAKHCFCKAGDLLAVVE

Homology

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BLAST of MC05g0222 vs. ExPASy Swiss-Prot
Match: Q9M1C2 (10 kDa chaperonin 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN10-1 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 1.4e-42
Identity = 90/141 (63.83%), Postives = 117/141 (82.98%), Query Frame = 0


Query: 3   MASSFVTVPKPFVD---RANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVL 62
           MASSF+TVPKPF+    + N+P++   +   G++R+S +++AVS K++PAKVVPQADRVL
Sbjct: 1   MASSFITVPKPFLSFPIKTNAPTLPQ-QTLLGIRRNSFRINAVSTKWEPAKVVPQADRVL 60

Query: 63  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 122
           VRLE L EKS+GGVLLPKSAVKFER+L GE++S+G+EVG  ++ PGKKVL SD++AYEVD
Sbjct: 61  VRLEVLPEKSSGGVLLPKSAVKFERYLTGEVVSVGSEVG--EVEPGKKVLFSDMSAYEVD 120

Query: 123 LGT-DAKHCFCKAGDLLAVVE 140
            GT DAKHCFCK  DLLA+V+
Sbjct: 121 FGTEDAKHCFCKESDLLAIVQ 138

[Close]

BLAST of MC05g0222 vs. ExPASy Swiss-Prot
Match: O80504 (10 kDa chaperonin 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN10-2 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 7.4e-39
Identity = 86/141 (60.99%), Postives = 111/141 (78.72%), Query Frame = 0


Query: 3   MASSFV-TVPKPFVD---RANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRV 62
           MAS+FV ++P PF     +A +PS ++     G +R  L++ A+S K++P KVVPQADRV
Sbjct: 1   MASTFVCSLPNPFFAFPVKATTPSTAN-HTLLGSRRGCLRIKAISTKWEPTKVVPQADRV 60

Query: 63  LVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEV 122
           LVRLE+L  KS+GGVLLPK+AVKFER+L GEIIS+G+EV G  + PGK+VL SD++AYEV
Sbjct: 61  LVRLEDLPIKSSGGVLLPKAAVKFERYLTGEIISVGSEV-GQQVGPGKRVLFSDVSAYEV 120

Query: 123 DLGTDAKHCFCKAGDLLAVVE 140
           DLGTDA+HCFCK  DLLA+VE
Sbjct: 121 DLGTDARHCFCKESDLLALVE 139

[Close]

BLAST of MC05g0222 vs. ExPASy Swiss-Prot
Match: Q0I7U2 (10 kDa chaperonin OS=Synechococcus sp. (strain CC9311) OX=64471 GN=groS PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 5.7e-07
Identity = 35/95 (36.84%), Postives = 52/95 (54.74%), Query Frame = 0


Query: 51  VVPQADRVLVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGND-------IA 110
           V P  DRV V++ E  EK+AGG+LLP +A   E+  VGE++ +G     +D       + 
Sbjct: 11  VKPLGDRVFVKISESEEKTAGGILLPDTAK--EKPQVGEVVQVGPGKSNDDGSRQAPEVG 70

Query: 111 PGKKVLISDINAYEVDLGTDAKHCFCKAGDLLAVV 139
            G KVL S     ++ LG+D ++      D+LAVV
Sbjct: 71  IGDKVLYSKYAGTDIKLGSD-EYVLLSEKDILAVV 102

[Close]

BLAST of MC05g0222 vs. ExPASy Swiss-Prot
Match: A5GNB0 (10 kDa chaperonin OS=Synechococcus sp. (strain WH7803) OX=32051 GN=groS PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 7.5e-07
Identity = 35/95 (36.84%), Postives = 51/95 (53.68%), Query Frame = 0


Query: 51  VVPQADRVLVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGND-------IA 110
           V P  DRV V++ E  EK+AGG+LLP +A   E+  VGE++ +G      D       + 
Sbjct: 11  VKPLGDRVFVKVSESEEKTAGGILLPDTAK--EKPQVGEVVQVGPGKANEDGSRQAPEVG 70

Query: 111 PGKKVLISDINAYEVDLGTDAKHCFCKAGDLLAVV 139
            G KVL S     ++ LG+D ++      D+LAVV
Sbjct: 71  VGDKVLYSKYAGTDIKLGSD-EYVLLSEKDILAVV 102

[Close]

BLAST of MC05g0222 vs. ExPASy Swiss-Prot
Match: Q7TUS3 (10 kDa chaperonin OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=groS PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.7e-06
Identity = 35/95 (36.84%), Postives = 51/95 (53.68%), Query Frame = 0


Query: 51  VVPQADRVLVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGND-------IA 110
           V P  DRV V++ E  EK+AGG+LLP +A   E+  VGE++ +G     +D       + 
Sbjct: 11  VKPLGDRVFVKVSESEEKTAGGILLPDTAK--EKPQVGEVVQVGPGKRNDDGSRQAPEVG 70

Query: 111 PGKKVLISDINAYEVDLGTDAKHCFCKAGDLLAVV 139
            G KVL S     ++ L TD ++      D+LAVV
Sbjct: 71  VGDKVLYSKYAGTDIKLSTD-EYVLLSEKDILAVV 102

[Close]

BLAST of MC05g0222 vs. NCBI nr
Match: XP_022146922.1 (10 kDa chaperonin 1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 268 bits (685), Expect = 2.58e-90
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVLV 60
           MAMASSFVTVPKPFVDRANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVLV
Sbjct: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVLV 60

Query: 61  RLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVDL 120
           RLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVDL
Sbjct: 61  RLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVDL 120

Query: 121 GTDAKHCFCKAGDLLAVVE 139
           GTDAKHCFCKAGDLLAVVE
Sbjct: 121 GTDAKHCFCKAGDLLAVVE 139

[Close]

BLAST of MC05g0222 vs. NCBI nr
Match: XP_022936135.1 (10 kDa chaperonin 1, chloroplastic-like [Cucurbita moschata] >KAG6597250.1 10 kDa chaperonin 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 223 bits (567), Expect = 2.61e-72
Identity = 117/140 (83.57%), Postives = 129/140 (92.14%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQ-KAGLQRSSLKVSAVSKKYKPAKVVPQADRVL 60
           MAMAS+FVTVPKPF D ANS SVSS +Q   G +RSSLKVSAVSKK++P KVVPQADRVL
Sbjct: 1   MAMASTFVTVPKPFNDWANSSSVSSRQQITGGFRRSSLKVSAVSKKWEPTKVVPQADRVL 60

Query: 61  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 120
           +RLEEL EKSAGGVLLPKSAVKFERHLVGEI+SIG+EVGG+D+APGKKV++SDINAYEVD
Sbjct: 61  LRLEELPEKSAGGVLLPKSAVKFERHLVGEILSIGSEVGGSDLAPGKKVILSDINAYEVD 120

Query: 121 LGTDAKHCFCKAGDLLAVVE 139
           LGTDAKHCFCKA DLLAVVE
Sbjct: 121 LGTDAKHCFCKASDLLAVVE 140

[Close]

BLAST of MC05g0222 vs. NCBI nr
Match: XP_023539001.1 (10 kDa chaperonin 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 222 bits (566), Expect = 3.71e-72
Identity = 117/140 (83.57%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQ-KAGLQRSSLKVSAVSKKYKPAKVVPQADRVL 60
           MAMAS+F+TVPKPF D ANS SVSS +Q   G +RSSLKVSAVSKK++P KVVPQADRVL
Sbjct: 1   MAMASTFLTVPKPFNDLANSSSVSSRQQITGGFRRSSLKVSAVSKKWEPTKVVPQADRVL 60

Query: 61  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 120
           +RLEEL EKSAGGVLLPKSAVKFERHLVGEI+SIG+EVGG D+APGKKVL+SDINAYEVD
Sbjct: 61  LRLEELPEKSAGGVLLPKSAVKFERHLVGEILSIGSEVGGTDLAPGKKVLLSDINAYEVD 120

Query: 121 LGTDAKHCFCKAGDLLAVVE 139
           LGTDAKHCFCKA DLLAVVE
Sbjct: 121 LGTDAKHCFCKASDLLAVVE 140

[Close]

BLAST of MC05g0222 vs. NCBI nr
Match: XP_022974766.1 (10 kDa chaperonin 1, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022974767.1 10 kDa chaperonin 1, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 221 bits (564), Expect = 7.49e-72
Identity = 117/140 (83.57%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQ-KAGLQRSSLKVSAVSKKYKPAKVVPQADRVL 60
           MAMAS+FVTVPKPF D ANS SVSS R    G +RSSLKVSAVSKK++P KVVPQADRVL
Sbjct: 1   MAMASTFVTVPKPFNDWANSSSVSSRRLITGGFRRSSLKVSAVSKKWEPTKVVPQADRVL 60

Query: 61  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 120
           +RLEEL EKSAGGVLLPKSAVKFERHLVGEI+SIG+EVGG+D+APGKKV++SDINAYEVD
Sbjct: 61  LRLEELPEKSAGGVLLPKSAVKFERHLVGEILSIGSEVGGSDLAPGKKVILSDINAYEVD 120

Query: 121 LGTDAKHCFCKAGDLLAVVE 139
           LGTDAKHCFCKA DLLAVVE
Sbjct: 121 LGTDAKHCFCKASDLLAVVE 140

[Close]

BLAST of MC05g0222 vs. NCBI nr
Match: XP_038880425.1 (10 kDa chaperonin 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 219 bits (557), Expect = 8.73e-71
Identity = 115/140 (82.14%), Postives = 129/140 (92.14%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQK-AGLQRSSLKVSAVSKKYKPAKVVPQADRVL 60
           MAMAS+FVTVPK  ++R NSPSVS+ R    GL+ S+LKVSA+SKK +PAKVVPQADRVL
Sbjct: 1   MAMASTFVTVPKLSINRPNSPSVSARRLIIGGLRSSTLKVSAISKKLEPAKVVPQADRVL 60

Query: 61  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 120
           VRLEEL EKSAGGVLLPKSAVKFER+LVGEI+S+GT+VGGNDIAPGKKVL+SD+NAYEVD
Sbjct: 61  VRLEELPEKSAGGVLLPKSAVKFERYLVGEILSVGTDVGGNDIAPGKKVLLSDMNAYEVD 120

Query: 121 LGTDAKHCFCKAGDLLAVVE 139
           LGTDAKHCFCKAGDLLAVVE
Sbjct: 121 LGTDAKHCFCKAGDLLAVVE 140

[Close]

BLAST of MC05g0222 vs. ExPASy TrEMBL
Match: A0A6J1D0Y0 (10 kDa chaperonin 1, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111016006 PE=4 SV=1)

HSP 1 Score: 268 bits (685), Expect = 1.25e-90
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVLV 60
           MAMASSFVTVPKPFVDRANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVLV
Sbjct: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVLV 60

Query: 61  RLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVDL 120
           RLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVDL
Sbjct: 61  RLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVDL 120

Query: 121 GTDAKHCFCKAGDLLAVVE 139
           GTDAKHCFCKAGDLLAVVE
Sbjct: 121 GTDAKHCFCKAGDLLAVVE 139

[Close]

BLAST of MC05g0222 vs. ExPASy TrEMBL
Match: A0A6J1FCS3 (10 kDa chaperonin 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442821 PE=4 SV=1)

HSP 1 Score: 223 bits (567), Expect = 1.26e-72
Identity = 117/140 (83.57%), Postives = 129/140 (92.14%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQ-KAGLQRSSLKVSAVSKKYKPAKVVPQADRVL 60
           MAMAS+FVTVPKPF D ANS SVSS +Q   G +RSSLKVSAVSKK++P KVVPQADRVL
Sbjct: 1   MAMASTFVTVPKPFNDWANSSSVSSRQQITGGFRRSSLKVSAVSKKWEPTKVVPQADRVL 60

Query: 61  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 120
           +RLEEL EKSAGGVLLPKSAVKFERHLVGEI+SIG+EVGG+D+APGKKV++SDINAYEVD
Sbjct: 61  LRLEELPEKSAGGVLLPKSAVKFERHLVGEILSIGSEVGGSDLAPGKKVILSDINAYEVD 120

Query: 121 LGTDAKHCFCKAGDLLAVVE 139
           LGTDAKHCFCKA DLLAVVE
Sbjct: 121 LGTDAKHCFCKASDLLAVVE 140

[Close]

BLAST of MC05g0222 vs. ExPASy TrEMBL
Match: A0A6J1ICA1 (10 kDa chaperonin 1, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111473490 PE=4 SV=1)

HSP 1 Score: 221 bits (564), Expect = 3.62e-72
Identity = 117/140 (83.57%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRANSPSVSSWRQ-KAGLQRSSLKVSAVSKKYKPAKVVPQADRVL 60
           MAMAS+FVTVPKPF D ANS SVSS R    G +RSSLKVSAVSKK++P KVVPQADRVL
Sbjct: 1   MAMASTFVTVPKPFNDWANSSSVSSRRLITGGFRRSSLKVSAVSKKWEPTKVVPQADRVL 60

Query: 61  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 120
           +RLEEL EKSAGGVLLPKSAVKFERHLVGEI+SIG+EVGG+D+APGKKV++SDINAYEVD
Sbjct: 61  LRLEELPEKSAGGVLLPKSAVKFERHLVGEILSIGSEVGGSDLAPGKKVILSDINAYEVD 120

Query: 121 LGTDAKHCFCKAGDLLAVVE 139
           LGTDAKHCFCKA DLLAVVE
Sbjct: 121 LGTDAKHCFCKASDLLAVVE 140

[Close]

BLAST of MC05g0222 vs. ExPASy TrEMBL
Match: A0A5D3CZW2 (10 kDa chaperonin isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003170 PE=4 SV=1)

HSP 1 Score: 217 bits (553), Expect = 1.78e-70
Identity = 115/141 (81.56%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRAN-SPSVSSWRQK-AGLQRSSLKVSAVSKKYKPAKVVPQADRV 60
           MAMAS+F+TVPKPF++  N SPSVS+ R    GL+ S+LKVSA+S+K++PAKVVPQADRV
Sbjct: 1   MAMASTFLTVPKPFINIPNTSPSVSARRLIIGGLRSSTLKVSAISRKWEPAKVVPQADRV 60

Query: 61  LVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEV 120
           LVRLEEL EKSAGGVLLPKSAVKFER+LVGEI+S+G EVGGNDIAPGKKVL+SDINAYEV
Sbjct: 61  LVRLEELPEKSAGGVLLPKSAVKFERYLVGEILSVGAEVGGNDIAPGKKVLLSDINAYEV 120

Query: 121 DLGTDAKHCFCKAGDLLAVVE 139
           DLGTDAKHCFCKAGDLLAVVE
Sbjct: 121 DLGTDAKHCFCKAGDLLAVVE 141

[Close]

BLAST of MC05g0222 vs. ExPASy TrEMBL
Match: A0A1S3AWL8 (10 kDa chaperonin isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483436 PE=4 SV=1)

HSP 1 Score: 217 bits (553), Expect = 1.78e-70
Identity = 115/141 (81.56%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 1   MAMASSFVTVPKPFVDRAN-SPSVSSWRQK-AGLQRSSLKVSAVSKKYKPAKVVPQADRV 60
           MAMAS+F+TVPKPF++  N SPSVS+ R    GL+ S+LKVSA+S+K++PAKVVPQADRV
Sbjct: 1   MAMASTFLTVPKPFINIPNTSPSVSARRLIIGGLRSSTLKVSAISRKWEPAKVVPQADRV 60

Query: 61  LVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEV 120
           LVRLEEL EKSAGGVLLPKSAVKFER+LVGEI+S+G EVGGNDIAPGKKVL+SDINAYEV
Sbjct: 61  LVRLEELPEKSAGGVLLPKSAVKFERYLVGEILSVGAEVGGNDIAPGKKVLLSDINAYEV 120

Query: 121 DLGTDAKHCFCKAGDLLAVVE 139
           DLGTDAKHCFCKAGDLLAVVE
Sbjct: 121 DLGTDAKHCFCKAGDLLAVVE 141

[Close]

BLAST of MC05g0222 vs. TAIR 10
Match: AT3G60210.1 (GroES-like family protein )

HSP 1 Score: 173.7 bits (439), Expect = 1.0e-43
Identity = 90/141 (63.83%), Postives = 117/141 (82.98%), Query Frame = 0


Query: 3   MASSFVTVPKPFVD---RANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRVL 62
           MASSF+TVPKPF+    + N+P++   +   G++R+S +++AVS K++PAKVVPQADRVL
Sbjct: 1   MASSFITVPKPFLSFPIKTNAPTLPQ-QTLLGIRRNSFRINAVSTKWEPAKVVPQADRVL 60

Query: 63  VRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEVD 122
           VRLE L EKS+GGVLLPKSAVKFER+L GE++S+G+EVG  ++ PGKKVL SD++AYEVD
Sbjct: 61  VRLEVLPEKSSGGVLLPKSAVKFERYLTGEVVSVGSEVG--EVEPGKKVLFSDMSAYEVD 120

Query: 123 LGT-DAKHCFCKAGDLLAVVE 140
            GT DAKHCFCK  DLLA+V+
Sbjct: 121 FGTEDAKHCFCKESDLLAIVQ 138

[Close]

BLAST of MC05g0222 vs. TAIR 10
Match: AT2G44650.1 (chloroplast chaperonin 10 )

HSP 1 Score: 161.4 bits (407), Expect = 5.3e-40
Identity = 86/141 (60.99%), Postives = 111/141 (78.72%), Query Frame = 0


Query: 3   MASSFV-TVPKPFVD---RANSPSVSSWRQKAGLQRSSLKVSAVSKKYKPAKVVPQADRV 62
           MAS+FV ++P PF     +A +PS ++     G +R  L++ A+S K++P KVVPQADRV
Sbjct: 1   MASTFVCSLPNPFFAFPVKATTPSTAN-HTLLGSRRGCLRIKAISTKWEPTKVVPQADRV 60

Query: 63  LVRLEELAEKSAGGVLLPKSAVKFERHLVGEIISIGTEVGGNDIAPGKKVLISDINAYEV 122
           LVRLE+L  KS+GGVLLPK+AVKFER+L GEIIS+G+EV G  + PGK+VL SD++AYEV
Sbjct: 61  LVRLEDLPIKSSGGVLLPKAAVKFERYLTGEIISVGSEV-GQQVGPGKRVLFSDVSAYEV 120

Query: 123 DLGTDAKHCFCKAGDLLAVVE 140
           DLGTDA+HCFCK  DLLA+VE
Sbjct: 121 DLGTDARHCFCKESDLLALVE 139

[Close]

BLAST of MC05g0222 vs. TAIR 10
Match: AT5G20720.1 (chaperonin 20 )

HSP 1 Score: 52.0 bits (123), Expect = 4.5e-07
Identity = 43/134 (32.09%), Postives = 71/134 (52.99%), Query Frame = 0


Query: 17  RANSPSVSSWR----QKAGLQRSSLK-VSAVSKKYKPAKVVPQADRVLVRLEELAEKSAG 76
           RA+S   SS +    +++  +R  +K  S V+ KY   K  P  DRVLV+++E  EK+ G
Sbjct: 25  RASSVKFSSLKPGTLRQSQFRRLVVKAASVVAPKYTSIK--PLGDRVLVKIKEAEEKTLG 84

Query: 77  GVLLPKSAVKFERHLVGEIISI--GTEVGGNDI----APGKKVLISDINAYEVDLGTDAK 136
           G+LLP +A    +   GE++++  G  +G N I      G +++ S     EV+   D K
Sbjct: 85  GILLPSTAQ--SKPQGGEVVAVGEGRTIGKNKIDITVPTGAQIIYSKYAGTEVEF-NDVK 144

Query: 137 HCFCKAGDLLAVVE 140
           H   K  D++ ++E
Sbjct: 145 HLILKEDDIVGILE 153

[Close]

BLAST of MC05g0222 vs. TAIR 10
Match: AT5G20720.2 (chaperonin 20 )

HSP 1 Score: 52.0 bits (123), Expect = 4.5e-07
Identity = 43/134 (32.09%), Postives = 71/134 (52.99%), Query Frame = 0


Query: 17  RANSPSVSSWR----QKAGLQRSSLK-VSAVSKKYKPAKVVPQADRVLVRLEELAEKSAG 76
           RA+S   SS +    +++  +R  +K  S V+ KY   K  P  DRVLV+++E  EK+ G
Sbjct: 25  RASSVKFSSLKPGTLRQSQFRRLVVKAASVVAPKYTSIK--PLGDRVLVKIKEAEEKTLG 84

Query: 77  GVLLPKSAVKFERHLVGEIISI--GTEVGGNDI----APGKKVLISDINAYEVDLGTDAK 136
           G+LLP +A    +   GE++++  G  +G N I      G +++ S     EV+   D K
Sbjct: 85  GILLPSTAQ--SKPQGGEVVAVGEGRTIGKNKIDITVPTGAQIIYSKYAGTEVEF-NDVK 144

Query: 137 HCFCKAGDLLAVVE 140
           H   K  D++ ++E
Sbjct: 145 HLILKEDDIVGILE 153

[Close]

BLAST of MC05g0222 vs. TAIR 10
Match: AT5G20720.3 (chaperonin 20 )

HSP 1 Score: 52.0 bits (123), Expect = 4.5e-07
Identity = 43/134 (32.09%), Postives = 71/134 (52.99%), Query Frame = 0


Query: 17  RANSPSVSSWR----QKAGLQRSSLK-VSAVSKKYKPAKVVPQADRVLVRLEELAEKSAG 76
           RA+S   SS +    +++  +R  +K  S V+ KY   K  P  DRVLV+++E  EK+ G
Sbjct: 25  RASSVKFSSLKPGTLRQSQFRRLVVKAASVVAPKYTSIK--PLGDRVLVKIKEAEEKTLG 84

Query: 77  GVLLPKSAVKFERHLVGEIISI--GTEVGGNDI----APGKKVLISDINAYEVDLGTDAK 136
           G+LLP +A    +   GE++++  G  +G N I      G +++ S     EV+   D K
Sbjct: 85  GILLPSTAQ--SKPQGGEVVAVGEGRTIGKNKIDITVPTGAQIIYSKYAGTEVEF-NDVK 144

Query: 137 HCFCKAGDLLAVVE 140
           H   K  D++ ++E
Sbjct: 145 HLILKEDDIVGILE 153

The following BLAST results are available for this feature:

BLAST of MC05g0222 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1C2	1.4e-42	63.83	10 kDa chaperonin 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN10-1 PE...	[more]
O80504	7.4e-39	60.99	10 kDa chaperonin 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN10-2 PE...	[more]
Q0I7U2	5.7e-07	36.84	10 kDa chaperonin OS=Synechococcus sp. (strain CC9311) OX=64471 GN=groS PE=3 SV=...	[more]
A5GNB0	7.5e-07	36.84	10 kDa chaperonin OS=Synechococcus sp. (strain WH7803) OX=32051 GN=groS PE=3 SV=...	[more]
Q7TUS3	1.7e-06	36.84	10 kDa chaperonin OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=groS ...	[more]

BLAST of MC05g0222 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146922.1	2.58e-90	100.00	10 kDa chaperonin 1, chloroplastic-like [Momordica charantia]	[more]
XP_022936135.1	2.61e-72	83.57	10 kDa chaperonin 1, chloroplastic-like [Cucurbita moschata] >KAG6597250.1 10 kD...	[more]
XP_023539001.1	3.71e-72	83.57	10 kDa chaperonin 1, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022974766.1	7.49e-72	83.57	10 kDa chaperonin 1, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022974...	[more]
XP_038880425.1	8.73e-71	82.14	10 kDa chaperonin 1, chloroplastic-like [Benincasa hispida]	[more]

BLAST of MC05g0222 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D0Y0	1.25e-90	100.00	10 kDa chaperonin 1, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1FCS3	1.26e-72	83.57	10 kDa chaperonin 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1ICA1	3.62e-72	83.57	10 kDa chaperonin 1, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A5D3CZW2	1.78e-70	81.56	10 kDa chaperonin isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3AWL8	1.78e-70	81.56	10 kDa chaperonin isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483436 PE=4 SV=1	[more]

BLAST of MC05g0222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G60210.1	1.0e-43	63.83	GroES-like family protein	[more]
AT2G44650.1	5.3e-40	60.99	chloroplast chaperonin 10	[more]
AT5G20720.1	4.5e-07	32.09	chaperonin 20	[more]
AT5G20720.2	4.5e-07	32.09	chaperonin 20	[more]
AT5G20720.3	4.5e-07	32.09	chaperonin 20	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020818	GroES chaperonin family	SMART	SM00883	Cpn10_2	coord: 50..138 e-value: 6.4E-11 score: 52.3
IPR020818	GroES chaperonin family	PFAM	PF00166	Cpn10	coord: 50..138 e-value: 3.0E-14 score: 52.8
IPR020818	GroES chaperonin family	PANTHER	PTHR10772	10 KDA HEAT SHOCK PROTEIN	coord: 16..138
IPR020818	GroES chaperonin family	CDD	cd00320	cpn10	coord: 50..138 e-value: 4.98774E-16 score: 66.3767
IPR037124	GroES chaperonin superfamily	GENE3D	2.30.33.40	GroES chaperonin	coord: 44..139 e-value: 1.7E-13 score: 52.3
None	No IPR available	PANTHER	PTHR10772:SF13	10 KDA CHAPERONIN 1, CHLOROPLASTIC-RELATED	coord: 16..138
IPR011032	GroES-like superfamily	SUPERFAMILY	50129	GroES-like	coord: 50..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0222.1	MC05g0222.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051085	chaperone cofactor-dependent protein refolding
biological_process	GO:0006457	protein folding
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051082	unfolded protein binding