MC05g0132 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0132
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255
Location	MC05: 930681 .. 934113 (-)
RNA-Seq Expression	MC05g0132
Synteny	MC05g0132

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CATGCCCAGAATTTACATTCAATTGGATTTGATTAGACAAAAAAGTTTCTACTTTATTGTTTGTACCATAAATTTTTCTTATAAATAATCGTAAATGTTATTAGATCCCACGTTATTTTTAAATAATGTTTAAAAATGATAACTCGAATATAGCTCAAACATATATTAAAAAATCGTGAGTTCGAATCACATCCATGTTGTCGTAACGAAAAGATAAAAAAAAATAAAAACATTAAAAATGAATTGAAACCCTCGAAAAGTTAAAAGAGAAAAAAATAATAATAAAAAAGGGGCACAGCAGAAGCACAACTGAAAGAGAAGTGTGACCTAATTTTGGAATGTTTTGAACAATTTCTGGAAAAATGGGATTATCATCGGCAATAGCATGGATGAATTTGAGCCCAATATTGAACCCGAAATTCCGGGCACCGGGCCGGGGCCGGAATCGGATCCTTTTCCTTGCTACTGCTTTCGCCACCATTCATCCCACGCTATCTCCTCACCACCCATGTCACCGCCATTACCATTCCCATTACTCACCAGTCCGCTGCTTATTCCCCACTCCCATGGCCAATTTCTCCTCCGCCGTCTCTCCCCGCCGCCGCCTCCGGGGAGTCGTCTTCGACATGGACGGCACCCTCACTGTCCCCGTCATCGATTTCGCCGCTATGTATAAATCCGTGCTTGGCCACGACGAGCATGATCGAATCAGAGCTCAGAACCCATCGGGAATCGATATTTTGCACATTATTCAGAATTGGGCTCCCGATAAGCAGCGCAGGGCGTACGAAGTCATCGCCGATTTCGAGCGCCAGGGACTTGATCGCCTCCGAATTATGCCCGGTCAGTGCCGGCCCTAGCTTCTCCTTCTCCACTTCACTGTGTTCGTTCTTTACTAACGCCTTTTTATCAGCAGCTTAGATAGATGGGAGATTTTACCCCTCCAGATTCCATGTTACTTATAATCTCAGAAGTTAGGTCTTTGGTTATCTCAACCAAATTCTTGATCATTATACGTGCAGGTGCTGCAGAACTTTGTGCCTTTCTTGATTCTAAGAAAATAAGGTAATCGCTTTCTTCTCCCCATGGTTGGTTGCTTGCATTTTGTGATCAGTATGTTTCCGTGATATACTTAAAAGTTGGTAGAATCATATACCCCACCCCCCACCCCACATACACTGATTATTCATTTTAACTTACCAGAAAAATATATATATATAAAAGGAAAAAGGATGCTAGGAGAAGAGGAAAAGAAGGAAGATAAGGGTCCAACATGGTGATTCTTTTAAATTTATTTGGTATTCGTCTCACGAGATGCTTGTGAATTTATAAAAGATGTTAAAAGCGAAATTTTGCAATTCACTGCATTTCGAGTAAACGGACTTAGTGATAACTTTGTGTCCACTTGCACAAGGCTAGGAACATTTGTTGTGCAAATGTATGTGGTGTTACATTTTATTTCTTTCTCCCTTTTTTTGATTTGTAATGCCAATGTGTTTTATCATTTGCTAACATTTTAGGACGGGACTAATCACTCGCAATATAAAGGAAGCTGTTGATCTTTTTCATCAGCGTTTTGGTGTAAGTTTATTGAATTTTCTTATAAGACTCTCTGTCAGAATTATAATTATAGTTATCTGTGATCTTTTGGAATTGGAGCAACTTGAGCTATTAGGAAGTCGCCGGTTCACAGATAGTGAGACCTACAAATATGTAGATCAAAATTCAATTGATTTCCCAACATAGTGTTCCATTATTATAGAACGAGGGATTTGGAGCAAGAGGTTTCTCTTCCTAATCACAACCACAGTAATTAATTTACTTTCGAATAACTATAATAGTGCTTTCATCCTGATTTGGGCAGTTGGGACTTAATTTCATCTGTTGTGTTCAGGCCTCAGGGATCCAACACTCTTGTCACCTACTTAGGACATTAAAACATGGAAAATCCTAGAGTCCCTTAGAAGATTTCTCTGTTGATGATTCTCCCTTCAAGCATTATTTGCGTAAAATGGCTGGCGGCTCTGCATGTCTTGGACTAGCAACTTCTCAATTGCTAGCCCACACTGCTATGGCCTTAGAAATCAAGGTCTTTCTACTGACAGAAAAAGTTTCCCTTTTATTCATGTTCTCTCTCTCTCAAAAACTCTGCAAATCAAGAGCTCCCAAATTTAATGCATGAGTATTTAAAGTGGAGACTCAAAACCACCTTCTTTTTCACTGTCAATTGGCTTCTCGAATTTGGAAGTATATCTTCCCAGAGTGTGGATGGTTTGCTCCTGGCGAACGGCTCTCTTTTCTTCCTCCTTGAAGGTCATGCATTCAAAAAGCAAGCTAAAATCCTCTGGTTCAGTTTTACTAGATCTTTTCTTTGGACTTATGGAACGAAAGGAACAAAATAATCTTCACTTTGATGGGAAAATCACTTTTTTGTGGGATCAAATCACTATTGTGGATATGTCTTGGTGTAAACACAACCACCCTTTTTGTAATTATAGTACCACTTCTCATTGTACGAATTGAAAAATCTTTTCCTCTTAACTTCCTTGGTTAGGGTCGAGGCTTCCCTCCCCCATTTATGTTATCCTCAGTTCATTTTCTGATGAAATTGCTTGCACTAAAAAAGTTTGAAATCCTTGTCCTGATGTTGTCCTTGGTAGTAATTACTAGCTTCTGCTGCAGCGGACATTTTATCCAGCACTAAGCAGAGAATTTGGTTCCTATAAACCAAATCCGGCCCCTCTTCTACATATCTGCTCTTCTTGGGATGTCTTGCCTAATGAAGTCATTATGGTCGGCGATAGCCTCAGAGATGATGTGAGTGTATTTGATCAGTTAATTAGGTTTTCAACTGATATTACTTCTCTAGTTCTTTCTCTCATACTACCGTAGGATATGTAGTTTGCTCTTATATGTGAAAAATTGGCTTCCTTATTCACTTATGGCAGGTGGGATGTGGCAAGCAAGCAGGAGCTTTCACATGTTTGCTTGACGAAACAGGAAGGTACAACCCAGCGGATTATGCCAACCTGGATCTCGAACCAGATTTTAAAGTATCATCGCTCGATGAAGTTCGTCATCTCTTGGATGCAGAATTCGATCTGGCGCCATGATCCCATCTCTGCCGATGGCCTCGATTGATTGGGAATTTTACTTGGGAAGTTGAGTCATTTATTGGGACTGGAAGGGAACCAGAGATTTTGGTTCCTGTGATTTAAAACATTTAGAATGGTGTGTTGAACAAAGTGGTGGTACTGGTAGTGAAGTGAATGTTGTATTTTGCCAATCATTTGTGGCTTTTGGTTGTTGTGATTCAGTACTGTTCATATAAGATCACATCAAACAAGGCTGTTGTATTAGTATGAGCTATACTTTGGTTAGCTTCTTTCAATAGACTTCATTGAAAATTTGAAAAATTTTAATATGTTTGAGG

mRNA sequence

CATGCCCAGAATTTACATTCAATTGGATTTGATTAGACAAAAAAGTTTCTACTTTATTGTTTGTACCATAAATTTTTCTTATAAATAATCGTAAATGTTATTAGATCCCACGTTATTTTTAAATAATGTTTAAAAATGATAACTCGAATATAGCTCAAACATATATTAAAAAATCGTGAGTTCGAATCACATCCATGTTGTCGTAACGAAAAGATAAAAAAAAATAAAAACATTAAAAATGAATTGAAACCCTCGAAAAGTTAAAAGAGAAAAAAATAATAATAAAAAAGGGGCACAGCAGAAGCACAACTGAAAGAGAAGTGTGACCTAATTTTGGAATGTTTTGAACAATTTCTGGAAAAATGGGATTATCATCGGCAATAGCATGGATGAATTTGAGCCCAATATTGAACCCGAAATTCCGGGCACCGGGCCGGGGCCGGAATCGGATCCTTTTCCTTGCTACTGCTTTCGCCACCATTCATCCCACGCTATCTCCTCACCACCCATGTCACCGCCATTACCATTCCCATTACTCACCAGTCCGCTGCTTATTCCCCACTCCCATGGCCAATTTCTCCTCCGCCGTCTCTCCCCGCCGCCGCCTCCGGGGAGTCGTCTTCGACATGGACGGCACCCTCACTGTCCCCGTCATCGATTTCGCCGCTATGTATAAATCCGTGCTTGGCCACGACGAGCATGATCGAATCAGAGCTCAGAACCCATCGGGAATCGATATTTTGCACATTATTCAGAATTGGGCTCCCGATAAGCAGCGCAGGGCGTACGAAGTCATCGCCGATTTCGAGCGCCAGGGACTTGATCGCCTCCGAATTATGCCCGGTGCTGCAGAACTTTGTGCCTTTCTTGATTCTAAGAAAATAAGGACGGGACTAATCACTCGCAATATAAAGGAAGCTGTTGATCTTTTTCATCAGCGTTTTGGTCGGACATTTTATCCAGCACTAAGCAGAGAATTTGGTTCCTATAAACCAAATCCGGCCCCTCTTCTACATATCTGCTCTTCTTGGGATGTCTTGCCTAATGAAGTCATTATGGTCGGCGATAGCCTCAGAGATGATGTGGGATGTGGCAAGCAAGCAGGAGCTTTCACATGTTTGCTTGACGAAACAGGAAGGTACAACCCAGCGGATTATGCCAACCTGGATCTCGAACCAGATTTTAAAGTATCATCGCTCGATGAAGTTCGTCATCTCTTGGATGCAGAATTCGATCTGGCGCCATGATCCCATCTCTGCCGATGGCCTCGATTGATTGGGAATTTTACTTGGGAAGTTGAGTCATTTATTGGGACTGGAAGGGAACCAGAGATTTTGGTTCCTGTGATTTAAAACATTTAGAATGGTGTGTTGAACAAAGTGGTGGTACTGGTAGTGAAGTGAATGTTGTATTTTGCCAATCATTTGTGGCTTTTGGTTGTTGTGATTCAGTACTGTTCATATAAGATCACATCAAACAAGGCTGTTGTATTAGTATGAGCTATACTTTGGTTAGCTTCTTTCAATAGACTTCATTGAAAATTTGAAAAATTTTAATATGTTTGAGG

Coding sequence (CDS)

ATGGGATTATCATCGGCAATAGCATGGATGAATTTGAGCCCAATATTGAACCCGAAATTCCGGGCACCGGGCCGGGGCCGGAATCGGATCCTTTTCCTTGCTACTGCTTTCGCCACCATTCATCCCACGCTATCTCCTCACCACCCATGTCACCGCCATTACCATTCCCATTACTCACCAGTCCGCTGCTTATTCCCCACTCCCATGGCCAATTTCTCCTCCGCCGTCTCTCCCCGCCGCCGCCTCCGGGGAGTCGTCTTCGACATGGACGGCACCCTCACTGTCCCCGTCATCGATTTCGCCGCTATGTATAAATCCGTGCTTGGCCACGACGAGCATGATCGAATCAGAGCTCAGAACCCATCGGGAATCGATATTTTGCACATTATTCAGAATTGGGCTCCCGATAAGCAGCGCAGGGCGTACGAAGTCATCGCCGATTTCGAGCGCCAGGGACTTGATCGCCTCCGAATTATGCCCGGTGCTGCAGAACTTTGTGCCTTTCTTGATTCTAAGAAAATAAGGACGGGACTAATCACTCGCAATATAAAGGAAGCTGTTGATCTTTTTCATCAGCGTTTTGGTCGGACATTTTATCCAGCACTAAGCAGAGAATTTGGTTCCTATAAACCAAATCCGGCCCCTCTTCTACATATCTGCTCTTCTTGGGATGTCTTGCCTAATGAAGTCATTATGGTCGGCGATAGCCTCAGAGATGATGTGGGATGTGGCAAGCAAGCAGGAGCTTTCACATGTTTGCTTGACGAAACAGGAAGGTACAACCCAGCGGATTATGCCAACCTGGATCTCGAACCAGATTTTAAAGTATCATCGCTCGATGAAGTTCGTCATCTCTTGGATGCAGAATTCGATCTGGCGCCATGA

Protein sequence

MGLSSAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSPVRCLFPTPMANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQNPSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP

Homology

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BLAST of MC05g0132 vs. ExPASy Swiss-Prot
Match: Q8RYE9 (Haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Arabidopsis thaliana OX=3702 GN=At2g33255 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-92
Identity = 153/226 (67.70%), Postives = 194/226 (85.84%), Query Frame = 0


Query: 69  MANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQNPSGIDILH 128
           MAN ++  + + RLRGVVFDMDGTLTVPVIDFAAMY++VLG D + RI+A++PSGIDILH
Sbjct: 22  MANLTT--NAKTRLRGVVFDMDGTLTVPVIDFAAMYRAVLGEDAYKRIKAESPSGIDILH 81

Query: 129 IIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNIKEAVD 188
            I++W+PDKQ++AYE+IAD+E+QG+D+L+IMPG AELC FLDSKKI+ GLITRN+++A+D
Sbjct: 82  HIESWSPDKQQKAYEIIADYEKQGIDKLQIMPGTAELCGFLDSKKIKRGLITRNVQKAID 141

Query: 189 LFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGCGKQAG 248
           +FHQRF   F PAL REF  YKPNP PLLHICS+WD+ PNEV+MVGDSL+DD+ CGK+AG
Sbjct: 142 IFHQRFEVIFSPALGREFRPYKPNPDPLLHICSTWDIQPNEVMMVGDSLKDDIACGKRAG 201

Query: 249 AFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 295
           AFTCLLDETGRY P D++   L+PDFKV SL ++++LL+  FDL P
Sbjct: 202 AFTCLLDETGRYGPDDFSVSGLQPDFKVDSLSKIQNLLETNFDLNP 245

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BLAST of MC05g0132 vs. ExPASy Swiss-Prot
Match: Q12486 (Uncharacterized hydrolase YOR131C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YOR131C PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.3e-21
Identity = 62/180 (34.44%), Postives = 96/180 (53.33%), Query Frame = 0


Query: 80  RRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQNPSGIDILHIIQNWAPDKQR 139
           + ++ VVFDMDGTL +P        ++ +G ++           IDILH I     +K++
Sbjct: 11  KHIKAVVFDMDGTLCLPQPWMFPAMRNAIGLED---------KSIDILHFIDTLPTEKEK 70

Query: 140 R-AYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNIKEAVDLFHQRFGRT- 199
           + A++ I   E + +  ++  PG  ++  +L    I   + TRN+   V+ F +RF  + 
Sbjct: 71  KEAHDRIELVEAKAMKEMQPQPGLVDIMRYLTKNGISKNICTRNVGAPVETFVKRFIPSE 130

Query: 200 ---FYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGCGKQAGAFTCLL 255
              F   ++REF   KP P PLLHI S  ++ P E+IMVGDS  DD+  G+ AG FT LL
Sbjct: 131 LSRFDYIVTREFRPTKPQPDPLLHIASKLNIRPLEMIMVGDSF-DDMKSGRSAGCFTVLL 180

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BLAST of MC05g0132 vs. ExPASy Swiss-Prot
Match: Q88M11 (N-acetylmuramic acid 6-phosphate phosphatase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=mupP PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.6e-12
Identity = 60/221 (27.15%), Postives = 100/221 (45.25%), Query Frame = 0


Query: 81  RLRGVVFDMDGTLTVPVIDFAAMYKSVLGHD-----EHDRIRAQNPSGIDILHIIQNWAP 140
           RLR V+FDMDGTL     DF A+ +++L        + + IR     G   + +   +A 
Sbjct: 2   RLRAVLFDMDGTLLDTAPDFIAICQAMLAERGLPAVDDNLIRGVISGGARAM-VATAFAM 61

Query: 141 DKQRRAYEV-----IADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNIKEAVDLF 200
           D +   +E      +  ++R      ++  G AEL A ++   +  G++T       +  
Sbjct: 62  DPEADGFEALRLEFLERYQRDCAVHSKLFEGMAELLADIEKGNLLWGVVTNKPVRFAEPI 121

Query: 201 HQRFGRTFYPAL---SREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGCGKQA 260
            Q+ G     AL        + KP+P PL+  C + ++ P  V+ VGD LR D+  G+ A
Sbjct: 122 MQQLGLAERSALLICPDHVKNSKPDPEPLILACKTLNLDPASVLFVGDDLR-DIESGRDA 181

Query: 261 GAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDA 289
           G  T  +   G  +P D  N +   D  V    E+R ++D+
Sbjct: 182 GTRTAAV-RYGYIHPEDNPN-NWGADVVVDHPLELRKVIDS 218

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BLAST of MC05g0132 vs. ExPASy Swiss-Prot
Match: Q9HZ62 (N-acetylmuramic acid 6-phosphate phosphatase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mupP PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 9.0e-10
Identity = 53/198 (26.77%), Postives = 85/198 (42.93%), Query Frame = 0


Query: 79  RRRLRGVVFDMDGTLTVPVIDFAAMYKSV-----LGHDEHDRIRAQNPSG----IDILHI 138
           R RL+ V+FDMDGTL     DF A+ +++     L   +  R+R     G    +     
Sbjct: 3   RMRLKAVLFDMDGTLLDTAPDFIAITQAMRAAHGLPPVDEQRVRDVVSGGARAMVAAAFG 62

Query: 139 IQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNIKEAVDL 198
           +   +P+ +    E +  ++       R   G  EL A ++   +  G++T       + 
Sbjct: 63  LSLDSPEVEPLRQEFLDRYQEHCAVLSRPYDGIPELLAAIEKAGLIWGVVTNKPVRFAEP 122

Query: 199 FHQRFG---RTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGCGKQ 258
             QR G   R+            KP+P PLL  CS   + P+ V+ +GD LR D+  G+ 
Sbjct: 123 IMQRLGYAERSRVLVCPDHVTRSKPDPEPLLLACSQLGIDPSRVLFIGDDLR-DIESGRD 182

Query: 259 AGAFTCLLDETGRYNPAD 265
           AG  T  +   G  +P D
Sbjct: 183 AGTKTAAV-RYGYIHPED 198

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BLAST of MC05g0132 vs. ExPASy Swiss-Prot
Match: Q3J8A0 (Phosphoglycolate phosphatase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=Noc_2493 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.5e-09
Identity = 55/218 (25.23%), Postives = 94/218 (43.12%), Query Frame = 0


Query: 85  VVFDMDGTL--TVPVIDFAA---MYKSVLGHDEHDRIRAQNPSGIDIL---HIIQNWAPD 144
           ++ D+DGTL  +VP + F     M +  L      ++R    +G++ L    ++ N   +
Sbjct: 8   ILIDVDGTLVDSVPDLTFCTDTMMERLGLPLRGETKVRQWVGNGVERLIKRALVDNMEGE 67

Query: 145 KQRRAYE-----VIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNIKEAVDLFH 204
            +   Y+      +A +      R  + PG  E  A+L S+  R G +T    +      
Sbjct: 68  PEEDLYQKAETIFLALYADNTSKRSHLYPGVNEGLAWLKSQGYRVGCVTNKAAQFTYPLL 127

Query: 205 QRFGRTFYPAL---SREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGCGKQAG 264
              G   Y  +          KP+PAPLLH  S + + P + +M+GDS+ D      +A 
Sbjct: 128 TELGIIDYFEIVISGDTLPEKKPHPAPLLHAASHFGIAPEKALMIGDSISDVKAA--RAA 187

Query: 265 AFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLL 287
            F  +    G  +  D    D +PD  + SL E+++LL
Sbjct: 188 NFQIVCLSYGYNHGVDIR--DSQPDSVIDSLIEIKNLL 221

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BLAST of MC05g0132 vs. NCBI nr
Match: XP_022157990.1 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 [Momordica charantia])

HSP 1 Score: 607 bits (1566), Expect = 1.72e-219
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0


Query: 1   MGLSSAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSP 60
           MGLSSAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSP
Sbjct: 1   MGLSSAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSP 60

Query: 61  VRCLFPTPMANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQN 120
           VRCLFPTPMANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQN
Sbjct: 61  VRCLFPTPMANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQN 120

Query: 121 PSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLIT 180
           PSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLIT
Sbjct: 121 PSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLIT 180

Query: 181 RNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDD 240
           RNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDD
Sbjct: 181 RNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDD 240

Query: 241 VGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           VGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP
Sbjct: 241 VGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294

[Close]

BLAST of MC05g0132 vs. NCBI nr
Match: XP_022924791.1 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 [Cucurbita moschata])

HSP 1 Score: 432 bits (1112), Expect = 2.22e-150
Identity = 227/297 (76.43%), Postives = 249/297 (83.84%), Query Frame = 0


Query: 1   MGLSSAIAWMNL--SPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHY 60
           M LSS+  W+NL  SP+ NP  RA GR R   +FL +AF+TIH TLS +  CH       
Sbjct: 4   MRLSSS-GWLNLKLSPVFNPICRAAGRNR---IFLLSAFSTIHSTLSLYSSCHHP----S 63

Query: 61  SPVRCLFPTPMANFSSAVS-PRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIR 120
           SP R  FP P  NFSS VS PR RLRGVVFDMDGTLTVPVIDFAAM+KSVLG +E+ RI+
Sbjct: 64  SPHRYSFPMP--NFSSTVSAPRGRLRGVVFDMDGTLTVPVIDFAAMHKSVLGDEEYVRIK 123

Query: 121 AQNPSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTG 180
           + NPSGIDILHIIQ+WAP+KQRRAYEVIADFERQG+DRL+IMPGAAELC FLDSK IR G
Sbjct: 124 SLNPSGIDILHIIQSWAPEKQRRAYEVIADFERQGIDRLQIMPGAAELCTFLDSKNIRRG 183

Query: 181 LITRNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSL 240
           LITRNIKEAVD+FHQRFG TF+PALSREFG YKPNPAPLLHICSSWDVLPNEVIMVGDSL
Sbjct: 184 LITRNIKEAVDIFHQRFGWTFHPALSREFGYYKPNPAPLLHICSSWDVLPNEVIMVGDSL 243

Query: 241 RDDVGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           RDDVGCGKQAGAFTCLLDETGRYN   YAN+DLEPDFKVS+L EV HLL+A F+LAP
Sbjct: 244 RDDVGCGKQAGAFTCLLDETGRYNSEHYANMDLEPDFKVSTLGEVLHLLNANFELAP 290

[Close]

BLAST of MC05g0132 vs. NCBI nr
Match: XP_022980371.1 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 [Cucurbita maxima])

HSP 1 Score: 432 bits (1111), Expect = 3.15e-150
Identity = 226/297 (76.09%), Postives = 249/297 (83.84%), Query Frame = 0


Query: 1   MGLSSAIAWMNL--SPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHY 60
           M LSS+  W+NL  SP+ NP  RA GR R   +FL +AF+TIH TLS +  CH       
Sbjct: 4   MRLSSS-GWLNLKWSPVFNPICRAAGRNR---IFLLSAFSTIHSTLSLYSSCHHP----S 63

Query: 61  SPVRCLFPTPMANFSSAVS-PRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIR 120
           SP R  FP P  NFSS VS PR RLRGVVFDMDGTLTVPVIDFAAM+KSVLG +E+ RI+
Sbjct: 64  SPHRYSFPMP--NFSSTVSAPRGRLRGVVFDMDGTLTVPVIDFAAMHKSVLGDEEYVRIK 123

Query: 121 AQNPSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTG 180
           + NPSGIDILHIIQ+WAP+KQRRAYEVIADFERQG+DRL+IMPGAAELC FLDSK IR G
Sbjct: 124 SLNPSGIDILHIIQSWAPEKQRRAYEVIADFERQGIDRLQIMPGAAELCTFLDSKNIRRG 183

Query: 181 LITRNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSL 240
           LITRNIKEAVD+FHQRFG TF+PALSREFG YKPNPAPLLHICSSWDVLPNEVIM+GDSL
Sbjct: 184 LITRNIKEAVDIFHQRFGWTFHPALSREFGYYKPNPAPLLHICSSWDVLPNEVIMIGDSL 243

Query: 241 RDDVGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           RDDVGCGKQAGAFTCLLDETGRYN   YAN+DLEPDFKVS+L EV HLL+A F+LAP
Sbjct: 244 RDDVGCGKQAGAFTCLLDETGRYNSEHYANMDLEPDFKVSTLGEVLHLLNANFELAP 290

[Close]

BLAST of MC05g0132 vs. NCBI nr
Match: KAG6582428.1 (Haloacid dehalogenase-like hydrolase domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 432 bits (1110), Expect = 4.47e-150
Identity = 223/297 (75.08%), Postives = 248/297 (83.50%), Query Frame = 0


Query: 1   MGLSSAIAWMNL--SPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHY 60
           M LSS+  W+NL  SP+ NP  RA GR R   +FL +AF+TIH TLS +  CH     H+
Sbjct: 4   MRLSSS-GWLNLKLSPVFNPICRAAGRNR---IFLLSAFSTIHSTLSLYSSCHHPSSPHH 63

Query: 61  SPVRCLFPTPMANFSSAVS-PRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIR 120
                 +  PM NFSS VS PR RLRGVVFDMDGTLTVPVIDFAAM+KSVLG +E+ RI+
Sbjct: 64  ------YSFPMPNFSSTVSAPRGRLRGVVFDMDGTLTVPVIDFAAMHKSVLGDEEYVRIK 123

Query: 121 AQNPSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTG 180
           + NPSGIDILHIIQ+WAP+KQRRAYEVIADFERQG+DRL+IMPGAAELC FLDSK IR G
Sbjct: 124 SLNPSGIDILHIIQSWAPEKQRRAYEVIADFERQGIDRLQIMPGAAELCTFLDSKNIRRG 183

Query: 181 LITRNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSL 240
           LITRNIKEAVD+FHQRFG TF+PALSREFG YKPNPAPLLHICSSWDVLPNEVIM+GDSL
Sbjct: 184 LITRNIKEAVDIFHQRFGWTFHPALSREFGYYKPNPAPLLHICSSWDVLPNEVIMIGDSL 243

Query: 241 RDDVGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           RDDVGCGKQAGAFTCLLDETGRYN   YAN+DLEPDFKVS+L EV HLL+A F+LAP
Sbjct: 244 RDDVGCGKQAGAFTCLLDETGRYNSEHYANMDLEPDFKVSTLGEVLHLLNANFELAP 290

[Close]

BLAST of MC05g0132 vs. NCBI nr
Match: KAG7018826.1 (Haloacid dehalogenase-like hydrolase domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 432 bits (1110), Expect = 4.47e-150
Identity = 226/297 (76.09%), Postives = 249/297 (83.84%), Query Frame = 0


Query: 1   MGLSSAIAWMNL--SPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHY 60
           M LSS+  W+NL  SP+ NP  RA GR R   +FL +AF+TIH TLS +  CH       
Sbjct: 4   MRLSSS-GWLNLKWSPVFNPICRAAGRNR---IFLLSAFSTIHSTLSLYSSCHHP----S 63

Query: 61  SPVRCLFPTPMANFSSAVS-PRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIR 120
           SP R  FP P  NFSS VS PR RLRGVVFDMDGTLTVPVIDFAAM+KSVLG +E+ RI+
Sbjct: 64  SPHRYSFPMP--NFSSTVSAPRGRLRGVVFDMDGTLTVPVIDFAAMHKSVLGDEEYVRIK 123

Query: 121 AQNPSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTG 180
           + NPSGIDILHIIQ+WAP+KQRRAYEVIADFERQG+DRL+IMPGAAELC FLDSK IR G
Sbjct: 124 SLNPSGIDILHIIQSWAPEKQRRAYEVIADFERQGIDRLQIMPGAAELCTFLDSKNIRRG 183

Query: 181 LITRNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSL 240
           LITRNIKEAVD+FHQRFG TF+PALSREFG YKPNPAPLLHICSSWDVLPNEVIM+GDSL
Sbjct: 184 LITRNIKEAVDIFHQRFGWTFHPALSREFGYYKPNPAPLLHICSSWDVLPNEVIMIGDSL 243

Query: 241 RDDVGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           RDDVGCGKQAGAFTCLLDETGRYN   YAN+DLEPDFKVS+L EV HLL+A F+LAP
Sbjct: 244 RDDVGCGKQAGAFTCLLDETGRYNSDHYANMDLEPDFKVSTLGEVLHLLNANFELAP 290

[Close]

BLAST of MC05g0132 vs. ExPASy TrEMBL
Match: A0A6J1DUJ7 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Momordica charantia OX=3673 GN=LOC111024583 PE=4 SV=1)

HSP 1 Score: 607 bits (1566), Expect = 8.33e-220
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0


Query: 1   MGLSSAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSP 60
           MGLSSAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSP
Sbjct: 1   MGLSSAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSP 60

Query: 61  VRCLFPTPMANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQN 120
           VRCLFPTPMANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQN
Sbjct: 61  VRCLFPTPMANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQN 120

Query: 121 PSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLIT 180
           PSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLIT
Sbjct: 121 PSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLIT 180

Query: 181 RNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDD 240
           RNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDD
Sbjct: 181 RNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDD 240

Query: 241 VGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           VGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP
Sbjct: 241 VGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294

[Close]

BLAST of MC05g0132 vs. ExPASy TrEMBL
Match: A0A6J1EAF5 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucurbita moschata OX=3662 GN=LOC111432183 PE=4 SV=1)

HSP 1 Score: 432 bits (1112), Expect = 1.07e-150
Identity = 227/297 (76.43%), Postives = 249/297 (83.84%), Query Frame = 0


Query: 1   MGLSSAIAWMNL--SPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHY 60
           M LSS+  W+NL  SP+ NP  RA GR R   +FL +AF+TIH TLS +  CH       
Sbjct: 4   MRLSSS-GWLNLKLSPVFNPICRAAGRNR---IFLLSAFSTIHSTLSLYSSCHHP----S 63

Query: 61  SPVRCLFPTPMANFSSAVS-PRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIR 120
           SP R  FP P  NFSS VS PR RLRGVVFDMDGTLTVPVIDFAAM+KSVLG +E+ RI+
Sbjct: 64  SPHRYSFPMP--NFSSTVSAPRGRLRGVVFDMDGTLTVPVIDFAAMHKSVLGDEEYVRIK 123

Query: 121 AQNPSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTG 180
           + NPSGIDILHIIQ+WAP+KQRRAYEVIADFERQG+DRL+IMPGAAELC FLDSK IR G
Sbjct: 124 SLNPSGIDILHIIQSWAPEKQRRAYEVIADFERQGIDRLQIMPGAAELCTFLDSKNIRRG 183

Query: 181 LITRNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSL 240
           LITRNIKEAVD+FHQRFG TF+PALSREFG YKPNPAPLLHICSSWDVLPNEVIMVGDSL
Sbjct: 184 LITRNIKEAVDIFHQRFGWTFHPALSREFGYYKPNPAPLLHICSSWDVLPNEVIMVGDSL 243

Query: 241 RDDVGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           RDDVGCGKQAGAFTCLLDETGRYN   YAN+DLEPDFKVS+L EV HLL+A F+LAP
Sbjct: 244 RDDVGCGKQAGAFTCLLDETGRYNSEHYANMDLEPDFKVSTLGEVLHLLNANFELAP 290

[Close]

BLAST of MC05g0132 vs. ExPASy TrEMBL
Match: A0A6J1ITF1 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucurbita maxima OX=3661 GN=LOC111479757 PE=4 SV=1)

HSP 1 Score: 432 bits (1111), Expect = 1.53e-150
Identity = 226/297 (76.09%), Postives = 249/297 (83.84%), Query Frame = 0


Query: 1   MGLSSAIAWMNL--SPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHY 60
           M LSS+  W+NL  SP+ NP  RA GR R   +FL +AF+TIH TLS +  CH       
Sbjct: 4   MRLSSS-GWLNLKWSPVFNPICRAAGRNR---IFLLSAFSTIHSTLSLYSSCHHP----S 63

Query: 61  SPVRCLFPTPMANFSSAVS-PRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIR 120
           SP R  FP P  NFSS VS PR RLRGVVFDMDGTLTVPVIDFAAM+KSVLG +E+ RI+
Sbjct: 64  SPHRYSFPMP--NFSSTVSAPRGRLRGVVFDMDGTLTVPVIDFAAMHKSVLGDEEYVRIK 123

Query: 121 AQNPSGIDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTG 180
           + NPSGIDILHIIQ+WAP+KQRRAYEVIADFERQG+DRL+IMPGAAELC FLDSK IR G
Sbjct: 124 SLNPSGIDILHIIQSWAPEKQRRAYEVIADFERQGIDRLQIMPGAAELCTFLDSKNIRRG 183

Query: 181 LITRNIKEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSL 240
           LITRNIKEAVD+FHQRFG TF+PALSREFG YKPNPAPLLHICSSWDVLPNEVIM+GDSL
Sbjct: 184 LITRNIKEAVDIFHQRFGWTFHPALSREFGYYKPNPAPLLHICSSWDVLPNEVIMIGDSL 243

Query: 241 RDDVGCGKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           RDDVGCGKQAGAFTCLLDETGRYN   YAN+DLEPDFKVS+L EV HLL+A F+LAP
Sbjct: 244 RDDVGCGKQAGAFTCLLDETGRYNSEHYANMDLEPDFKVSTLGEVLHLLNANFELAP 290

[Close]

BLAST of MC05g0132 vs. ExPASy TrEMBL
Match: A0A6J1IGK1 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucurbita maxima OX=3661 GN=LOC111473209 PE=4 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 2.15e-144
Identity = 211/291 (72.51%), Postives = 237/291 (81.44%), Query Frame = 0


Query: 5   SAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSPVRCL 64
           S+  W+NLS   NPKFRA  +  +R  FL        P  + +H C RH    + P    
Sbjct: 18  SSSTWLNLSQNFNPKFRA--QRCHRSFFL--------PAFAANHSCRRH----FPPKPSS 77

Query: 65  FPTPMANFSSAVSPRR-RLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQNPSG 124
           FP  MANFSSAVS RR RLRGV+FDMDGTLTVPVIDFAAMYKSVLG +++ RI++ NPSG
Sbjct: 78  FPMAMANFSSAVSARRARLRGVIFDMDGTLTVPVIDFAAMYKSVLGDEDYARIKSLNPSG 137

Query: 125 IDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNI 184
           IDILHIIQ+WAP+KQRRAYEVIADFERQG++RL+IMPGAAELC FLDSK IR GLITRN+
Sbjct: 138 IDILHIIQSWAPEKQRRAYEVIADFERQGIERLQIMPGAAELCTFLDSKNIRRGLITRNV 197

Query: 185 KEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGC 244
           KEAVD+FHQRFG TF+PALSREFGSYKPNPAPLLHICSSWDVLPN+VIMVGDSL+DD+GC
Sbjct: 198 KEAVDIFHQRFGWTFHPALSREFGSYKPNPAPLLHICSSWDVLPNQVIMVGDSLKDDIGC 257

Query: 245 GKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           GKQAGAFTCLLDETGRYN   YAN DLEPDFK S+L EV HLL A F+L P
Sbjct: 258 GKQAGAFTCLLDETGRYNSEHYANTDLEPDFKASTLGEVLHLLKANFELVP 294

[Close]

BLAST of MC05g0132 vs. ExPASy TrEMBL
Match: A0A6J1GGH1 (haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucurbita moschata OX=3662 GN=LOC111454004 PE=4 SV=1)

HSP 1 Score: 415 bits (1067), Expect = 7.06e-144
Identity = 210/291 (72.16%), Postives = 235/291 (80.76%), Query Frame = 0


Query: 5   SAIAWMNLSPILNPKFRAPGRGRNRILFLATAFATIHPTLSPHHPCHRHYHSHYSPVRCL 64
           S+  W+NLS   NPKFRA  +  NR  FL        P  + +H C RH    + P    
Sbjct: 12  SSSTWLNLSQNFNPKFRA--QRCNRSFFL--------PAFAANHSCRRH----FPPKPSS 71

Query: 65  FPTPMANFSSAVSPRR-RLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQNPSG 124
           FP  MANFSSA S  R RLRGV+FDMDGTLTVPVIDFAAMYKSVLG +++ RI++ NPSG
Sbjct: 72  FPMAMANFSSAASAHRARLRGVIFDMDGTLTVPVIDFAAMYKSVLGDEDYARIKSLNPSG 131

Query: 125 IDILHIIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNI 184
           IDILHIIQ+WAP+KQRRAYEVIADFERQG++RL+IMPGAAELC FLDSK IR GLITRN+
Sbjct: 132 IDILHIIQSWAPEKQRRAYEVIADFERQGIERLQIMPGAAELCTFLDSKNIRRGLITRNV 191

Query: 185 KEAVDLFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGC 244
           KEAVD+FHQRFG TF+PALSREFGSYKPNPAPLLHICSSWDVLPN+VIMVGDSL+DD+GC
Sbjct: 192 KEAVDIFHQRFGWTFHPALSREFGSYKPNPAPLLHICSSWDVLPNQVIMVGDSLKDDIGC 251

Query: 245 GKQAGAFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 294
           GKQAGAFTCLLDETGRYN   YAN DLEPDFK S+L EV HLL A F+L P
Sbjct: 252 GKQAGAFTCLLDETGRYNSEHYANTDLEPDFKASTLGEVLHLLKANFELTP 288

[Close]

BLAST of MC05g0132 vs. TAIR 10
Match: AT2G33255.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 7.9e-94
Identity = 153/226 (67.70%), Postives = 194/226 (85.84%), Query Frame = 0


Query: 69  MANFSSAVSPRRRLRGVVFDMDGTLTVPVIDFAAMYKSVLGHDEHDRIRAQNPSGIDILH 128
           MAN ++  + + RLRGVVFDMDGTLTVPVIDFAAMY++VLG D + RI+A++PSGIDILH
Sbjct: 22  MANLTT--NAKTRLRGVVFDMDGTLTVPVIDFAAMYRAVLGEDAYKRIKAESPSGIDILH 81

Query: 129 IIQNWAPDKQRRAYEVIADFERQGLDRLRIMPGAAELCAFLDSKKIRTGLITRNIKEAVD 188
            I++W+PDKQ++AYE+IAD+E+QG+D+L+IMPG AELC FLDSKKI+ GLITRN+++A+D
Sbjct: 82  HIESWSPDKQQKAYEIIADYEKQGIDKLQIMPGTAELCGFLDSKKIKRGLITRNVQKAID 141

Query: 189 LFHQRFGRTFYPALSREFGSYKPNPAPLLHICSSWDVLPNEVIMVGDSLRDDVGCGKQAG 248
           +FHQRF   F PAL REF  YKPNP PLLHICS+WD+ PNEV+MVGDSL+DD+ CGK+AG
Sbjct: 142 IFHQRFEVIFSPALGREFRPYKPNPDPLLHICSTWDIQPNEVMMVGDSLKDDIACGKRAG 201

Query: 249 AFTCLLDETGRYNPADYANLDLEPDFKVSSLDEVRHLLDAEFDLAP 295
           AFTCLLDETGRY P D++   L+PDFKV SL ++++LL+  FDL P
Sbjct: 202 AFTCLLDETGRYGPDDFSVSGLQPDFKVDSLSKIQNLLETNFDLNP 245

The following BLAST results are available for this feature:

BLAST of MC05g0132 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYE9	1.1e-92	67.70	Haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Arab...	[more]
Q12486	1.3e-21	34.44	Uncharacterized hydrolase YOR131C OS=Saccharomyces cerevisiae (strain ATCC 20450...	[more]
Q88M11	5.6e-12	27.15	N-acetylmuramic acid 6-phosphate phosphatase OS=Pseudomonas putida (strain ATCC ...	[more]
Q9HZ62	9.0e-10	26.77	N-acetylmuramic acid 6-phosphate phosphatase OS=Pseudomonas aeruginosa (strain A...	[more]
Q3J8A0	4.5e-09	25.23	Phosphoglycolate phosphatase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 1...	[more]

BLAST of MC05g0132 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157990.1	1.72e-219	100.00	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 [Momord...	[more]
XP_022924791.1	2.22e-150	76.43	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 [Cucurb...	[more]
XP_022980371.1	3.15e-150	76.09	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 [Cucurb...	[more]
KAG6582428.1	4.47e-150	75.08	Haloacid dehalogenase-like hydrolase domain-containing protein, partial [Cucurbi...	[more]
KAG7018826.1	4.47e-150	76.09	Haloacid dehalogenase-like hydrolase domain-containing protein, partial [Cucurbi...	[more]

BLAST of MC05g0132 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUJ7	8.33e-220	100.00	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Momo...	[more]
A0A6J1EAF5	1.07e-150	76.43	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucu...	[more]
A0A6J1ITF1	1.53e-150	76.09	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucu...	[more]
A0A6J1IGK1	2.15e-144	72.51	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucu...	[more]
A0A6J1GGH1	7.06e-144	72.16	haloacid dehalogenase-like hydrolase domain-containing protein At2g33255 OS=Cucu...	[more]

BLAST of MC05g0132 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33255.1	7.9e-94	67.70	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006439	HAD hydrolase, subfamily IA	TIGRFAM	TIGR01549	TIGR01549	coord: 85..248 e-value: 5.0E-15 score: 54.2
None	No IPR available	GENE3D	1.10.260.80		coord: 95..156 e-value: 6.3E-44 score: 151.8
None	No IPR available	SFLD	SFLDG01129	C1.5:_HAD,_Beta-PGM,_Phosphat	coord: 83..289 e-value: 7.2E-24 score: 79.4
None	No IPR available	SFLD	SFLDS00003	Haloacid_Dehalogenase	coord: 83..289 e-value: 7.2E-24 score: 79.4
None	No IPR available	PANTHER	PTHR19288	4-NITROPHENYLPHOSPHATASE-RELATED	coord: 72..287
None	No IPR available	PANTHER	PTHR19288:SF74	OS02G0816100 PROTEIN	coord: 72..287
IPR041492	Haloacid dehalogenase-like hydrolase	PFAM	PF13419	HAD_2	coord: 85..251 e-value: 1.1E-15 score: 58.1
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 85..258 e-value: 6.3E-44 score: 151.8
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 67..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0132.1	MC05g0132.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity