MC04g_new0454 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g_new0454 vs. ExPASy Swiss-Prot
Match: P43298 (Receptor protein kinase TMK1 OS=Arabidopsis thaliana OX=3702 GN=TMK1 PE=1 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 576/951 (60.57%), Postives = 725/951 (76.24%), Query Frame = 0

Query: 13  FVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQWKHVKCDSENRV 72
           F+L SF    ++    +  DGD S M+ L+ S+  P+S GWS  D C+W H+ C    RV
Sbjct: 7   FLLFSFTFLLLLSLSKADSDGDLSAMLSLKKSLNPPSSFGWSDPDPCKWTHIVCTGTKRV 66

Query: 73  VKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLA--ESLQILLAHDNLFTSL 132
            +IQIG+  L+G+L  +L +LS L +LE+Q N +SGP P+L+   SLQ+L+  +N F S+
Sbjct: 67  TRIQIGHSGLQGTLSPDLRNLSELERLELQWNNISGPVPSLSGLASLQVLMLSNNNFDSI 126

Query: 133 PADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTIPDFFDGTTFP 192
           P+D F   ++LQ +EIDNNPF +W+IP+++R+AS LQ  SAN  N++G++P F     FP
Sbjct: 127 PSDVFQGLTSLQSVEIDNNPFKSWEIPESLRNASALQNFSANSANVSGSLPGFLGPDEFP 186

Query: 193 TLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMTNLQEIWLHTN 252
            L  LHL+ N LEG LP S +GS +QSLWLNGQ    KL G + +LQNMT L+E+WLH+N
Sbjct: 187 GLSILHLAFNNLEGELPMSLAGSQVQSLWLNGQ----KLTGDITVLQNMTGLKEVWLHSN 246

Query: 253 QFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQGPAPVFDRNV 312
           +FSGPLPDFS L+ L++LSLRDN  TG VP SL++L+SL +VNLTNN LQGP PVF  +V
Sbjct: 247 KFSGPLPDFSGLKELESLSLRDNSFTGPVPASLLSLESLKVVNLTNNHLQGPVPVFKSSV 306

Query: 313 QLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC-NFKGVVCIG 372
            +D+   +N+FC+ SPG  CDPRV +LL +A S ++P   A++WKGNDPC N+ G+ C  
Sbjct: 307 SVDLDKDSNSFCLSSPG-ECDPRVKSLLLIASSFDYPPRLAESWKGNDPCTNWIGIAC-- 366

Query: 373 NSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTMPSLVELDVS 432
            S  N+TV++   + L GTISP F  + S+ +++L  N L+G+IP EL T+P+L  LDVS
Sbjct: 367 -SNGNITVISLEKMELTGTISPEFGAIKSLQRIILGINNLTGMIPQELTTLPNLKTLDVS 426

Query: 433 NNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGLPVDVDK--- 492
           +N+L GKVP FR NV+VNT+GNPDIGKD+ S   PGS    SPS     G+  D D+   
Sbjct: 427 SNKLFGKVPGFRSNVVVNTNGNPDIGKDKSSLSSPGS---SSPSGGSGSGINGDKDRRGM 486

Query: 493 KSNS--GVVVGSII-GVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSG-DQNA 552
           KS++  G++VGS++ G++ + L+G ++F  Y++ ++R S  +S N VVVHP HSG D  +
Sbjct: 487 KSSTFIGIIVGSVLGGLLSIFLIGLLVFCWYKKRQKRFSGSESSNAVVVHPRHSGSDNES 546

Query: 553 VKITIAESRAD-GNAPETGRVPIGGPSDV----HVVEAGNMVISIQVLRNVTNNFSPENI 612
           VKIT+A S    G   +T  +P  G S+V     +VEAGNM+ISIQVLR+VTNNFS +NI
Sbjct: 547 VKITVAGSSVSVGGISDTYTLP--GTSEVGDNIQMVEAGNMLISIQVLRSVTNNFSSDNI 606

Query: 613 LGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYC 672
           LGSGGFG VYKGELHDGTKIAVKRME+GVI  KG  EFK+EIAVLTKVRHR+LV LLGYC
Sbjct: 607 LGSGGFGVVYKGELHDGTKIAVKRMENGVIAGKGFAEFKSEIAVLTKVRHRHLVTLLGYC 666

Query: 673 LDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSF 732
           LDGNE+LLVYEYMPQGTLSR LF W +EG+ PL WK+RL +ALDVARGVEYLH LAHQSF
Sbjct: 667 LDGNEKLLVYEYMPQGTLSRHLFEWSEEGLKPLLWKQRLTLALDVARGVEYLHGLAHQSF 726

Query: 733 IHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTK 792
           IHRDLKPSNILLGDDMRAKVADFGLVRLAPEGK S+ETR+AGTFGYLAPEYAVTGRVTTK
Sbjct: 727 IHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKGSIETRIAGTFGYLAPEYAVTGRVTTK 786

Query: 793 VDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKD-TFPKAIDPSIHLDQET 852
           VDVYSFGVILME+ITGRK+LD+SQPEE +HLVSWF+RM+INK+ +F KAID +I LD+ET
Sbjct: 787 VDVYSFGVILMELITGRKSLDESQPEESIHLVSWFKRMYINKEASFKKAIDTTIDLDEET 846

Query: 853 LGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQ 912
           L S+ TVA+LAGHCCAREPYQRPDM HAVN+LSSL+++WKP++ + E++ GIDL+MSLPQ
Sbjct: 847 LASVHTVAELAGHCCAREPYQRPDMGHAVNILSSLVELWKPSDQNPEDIYGIDLDMSLPQ 906

Query: 913 ALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 948
           ALKKWQA+EG S  D  SS+SS L S DNTQ SIP RP GFA SFTSVD R
Sbjct: 907 ALKKWQAYEGRS--DLESSTSSLLPSLDNTQMSIPTRPYGFAESFTSVDGR 942

BLAST of MC04g_new0454 vs. ExPASy Swiss-Prot
Match: Q9SIT1 (Receptor-like kinase TMK3 OS=Arabidopsis thaliana OX=3702 GN=TMK3 PE=1 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 1.3e-304
Identity = 550/957 (57.47%), Postives = 697/957 (72.83%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ 60
           M   H G   F+  L     F +     S    D S M  L++S+   + + WS  + C+
Sbjct: 1   MSNSHLGTLCFIISLLGLANFSL-----SQTGLDDSTMQSLKSSLNLTSDVDWSNPNPCK 60

Query: 61  WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLA--ESLQ 120
           W+ V+CD  NRV KIQ+  + ++G+LP+ L SLS LV LE+  N++SGP P+L+    LQ
Sbjct: 61  WQSVQCDGSNRVTKIQLKQKGIRGTLPTNLQSLSELVILELFLNRISGPIPDLSGLSRLQ 120

Query: 121 ILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITG 180
            L  HDNLFTS+P + F+  S+LQ + ++NNPF  W IPD +++A+ LQ ++ +  +I G
Sbjct: 121 TLNLHDNLFTSVPKNLFSGMSSLQEMYLENNPFDPWVIPDTVKEATSLQNLTLSNCSIIG 180

Query: 181 TIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQN 240
            IPDFF   + P+L NL LS N LEG LP SF+G+SIQSL+LNGQ    KLNGS+++L N
Sbjct: 181 KIPDFFGSQSLPSLTNLKLSQNGLEGELPMSFAGTSIQSLFLNGQ----KLNGSISVLGN 240

Query: 241 MTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNM 300
           MT+L E+ L  NQFSGP+PD S L  L+  ++R+NQLTG+VP SLV+L SL  VNLTNN 
Sbjct: 241 MTSLVEVSLQGNQFSGPIPDLSGLVSLRVFNVRENQLTGVVPQSLVSLSSLTTVNLTNNY 300

Query: 301 LQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGND 360
           LQGP P+F ++V +D+    N+FC +  G  CDPRV+ L+SVA+S  +P   A++WKGN+
Sbjct: 301 LQGPTPLFGKSVGVDIVNNMNSFCTNVAGEACDPRVDTLVSVAESFGYPVKLAESWKGNN 360

Query: 361 PC-NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTEL 420
           PC N+ G+ C G    N+TV+N R   L+GTISPS + LTS+  + L+DN LSG IP EL
Sbjct: 361 PCVNWVGITCSGG---NITVVNMRKQDLSGTISPSLAKLTSLETINLADNKLSGHIPDEL 420

Query: 421 VTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKD--RRSTPPPGSPTEKSPSDP 480
            T+  L  LDVSNN  +G  P FR  V + T+GN ++GK+   +++  PG+     PS  
Sbjct: 421 TTLSKLRLLDVSNNDFYGIPPKFRDTVTLVTEGNANMGKNGPNKTSDAPGASPGSKPSG- 480

Query: 481 GSGGLPVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNT-VVVHP 540
           GS G      K SN  ++V  + GV+G + +  +   LY + ++R +RVQSP++ +V+HP
Sbjct: 481 GSDGSETS-KKSSNVKIIVPVVGGVVGALCLVGLGVCLYAKKRKRPARVQSPSSNMVIHP 540

Query: 541 SHSGDQNAVKITIAESRAD-GNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFS 600
            HSGD + +K+T+A S  + G   ++        SD+HVVEAGN+VISIQVLRNVTNNFS
Sbjct: 541 HHSGDNDDIKLTVAASSLNSGGGSDSYSHSGSAASDIHVVEAGNLVISIQVLRNVTNNFS 600

Query: 601 PENILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVAL 660
            ENILG GGFGTVYKGELHDGTKIAVKRMES V+ +KGL EFK+EI VLTK+RHR+LVAL
Sbjct: 601 EENILGRGGFGTVYKGELHDGTKIAVKRMESSVVSDKGLTEFKSEITVLTKMRHRHLVAL 660

Query: 661 LGYCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLA 720
           LGYCLDGNERLLVYEYMPQGTLS+ LF+WK+EG  PL+W RRL +ALDVARGVEYLH+LA
Sbjct: 661 LGYCLDGNERLLVYEYMPQGTLSQHLFHWKEEGRKPLDWTRRLAIALDVARGVEYLHTLA 720

Query: 721 HQSFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGR 780
           HQSFIHRDLKPSNILLGDDMRAKV+DFGLVRLAP+GK S+ETR+AGTFGYLAPEYAVTGR
Sbjct: 721 HQSFIHRDLKPSNILLGDDMRAKVSDFGLVRLAPDGKYSIETRVAGTFGYLAPEYAVTGR 780

Query: 781 VTTKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKD--TFPKAIDPSIH 840
           VTTKVD++S GVILME+ITGRKALD++QPE+ +HLV+WFRR+  +KD   F  AIDP+I 
Sbjct: 781 VTTKVDIFSLGVILMELITGRKALDETQPEDSVHLVTWFRRVAASKDENAFKNAIDPNIS 840

Query: 841 LDQETLGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLE 900
           LD +T+ SI  V +LAGHCCAREPYQRPDM+H VNVLSSL   WKPTE D ++V GID +
Sbjct: 841 LDDDTVASIEKVWELAGHCCAREPYQRPDMAHIVNVLSSLTVQWKPTETDPDDVYGIDYD 900

Query: 901 MSLPQALKKWQAFEG-NSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 948
           M LPQ LKKWQAFEG +   D S SSSS   S DNTQTSIP RPSGFA+SFTSVD R
Sbjct: 901 MPLPQVLKKWQAFEGLSQTADDSGSSSSAYGSKDNTQTSIPTRPSGFADSFTSVDGR 943

BLAST of MC04g_new0454 vs. ExPASy Swiss-Prot
Match: Q9FYK0 (Receptor-like kinase TMK2 OS=Arabidopsis thaliana OX=3702 GN=TMK2 PE=1 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 2.3e-264
Identity = 500/924 (54.11%), Postives = 640/924 (69.26%), Query Frame = 0

Query: 34  DGSVMVILRTSIGAPASLGWSGSDYCQWK-HVKCDSENRVVKIQIGNQNLKGSLPSELFS 93
           D +VM+ LR S+    +  WSGSD C+W   +KCD+ NRV  IQIG++ + G LP +L  
Sbjct: 23  DEAVMIALRDSLKLSGNPNWSGSDPCKWSMFIKCDASNRVTAIQIGDRGISGKLPPDLGK 82

Query: 94  LSALVQLEVQGNQLSGPFPNLA--ESLQILLAHDNLFTSLPADFFAKKSTLQMIEIDNNP 153
           L++L + EV  N+L+GP P+LA  +SL  + A+DN FTS+P DFF+  S+LQ + +DNNP
Sbjct: 83  LTSLTKFEVMRNRLTGPIPSLAGLKSLVTVYANDNDFTSVPEDFFSGLSSLQHVSLDNNP 142

Query: 154 FSAWKIPDNIRDASGLQEISANRVNITGTIPDF-FDGTTFPTLRNLHLSGNFLEGGLPAS 213
           F +W IP ++ +A+ L + SA   N++G IPD+ F+G  F +L  L LS N L    P +
Sbjct: 143 FDSWVIPPSLENATSLVDFSAVNCNLSGKIPDYLFEGKDFSSLTTLKLSYNSLVCEFPMN 202

Query: 214 FSGSSIQSLWLNGQQSNFKLNGSVAILQNMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLS 273
           FS S +Q L LNGQ+   KL+GS++ LQ MT+L  + L  N FSGPLPDFS L  L++ +
Sbjct: 203 FSDSRVQVLMLNGQKGREKLHGSISFLQKMTSLTNVTLQGNSFSGPLPDFSGLVSLKSFN 262

Query: 274 LRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQGPAPVFDR-NVQLDMRPGTNNFCVDSPGV 333
           +R+NQL+G+VP SL  L+SL  V L NN+LQGP P F   +++ D+  G N+FC+D+PG 
Sbjct: 263 VRENQLSGLVPSSLFELQSLSDVALGNNLLQGPTPNFTAPDIKPDLN-GLNSFCLDTPGT 322

Query: 334 PCDPRVNALLSVAQSMNFPTVFAKNWKGNDPCN-FKGVVCIGNSPVNVTVLNFRNLGLAG 393
            CDPRVN LLS+ ++  +P  FA+ WKGNDPC+ + G+ C G    ++TV+NF+NLGL G
Sbjct: 323 SCDPRVNTLLSIVEAFGYPVNFAEKWKGNDPCSGWVGITCTG---TDITVINFKNLGLNG 382

Query: 394 TISPSFSLLTSVTKLLLSDNLLSGVIPTELVTMPSLVELDVSNNRLHGKVPSFRKNVIVN 453
           TISP F+   S+  + LS N L+G IP EL  + +L  LDVS NRL G+VP F    IVN
Sbjct: 383 TISPRFADFASLRVINLSQNNLNGTIPQELAKLSNLKTLDVSKNRLCGEVPRF-NTTIVN 442

Query: 454 TDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGLPVDVDKKSNSGVVVGSIIGV-IGLVLV 513
           T GN +         P G+  +K+                SN+G +VGS+IG+ + L+L+
Sbjct: 443 TTGNFE-------DCPNGNAGKKA---------------SSNAGKIVGSVIGILLALLLI 502

Query: 514 GAVIFFLYRRNKRRSSRVQSPNTVVVHP-SHSGDQNAVKITIAESRADGNAPETGRVPIG 573
           G  IFFL ++  +            +HP   S DQ+A KITI E+   G   E+G     
Sbjct: 503 GVAIFFLVKKKMQYHK---------MHPQQQSSDQDAFKITI-ENLCTG-VSESG----F 562

Query: 574 GPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGSGGFGTVYKGELHDGTKIAVKRMESG 633
             +D H+ EAGN+VISIQVLR+ T NF  +NILG GGFG VYKGELHDGTKIAVKRMES 
Sbjct: 563 SGNDAHLGEAGNIVISIQVLRDATYNFDEKNILGRGGFGIVYKGELHDGTKIAVKRMESS 622

Query: 634 VIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDGNERLLVYEYMPQGTLSRFLFYWKKE 693
           +I  KGL+EFK+EIAVLT+VRHRNLV L GYCL+GNERLLVY+YMPQGTLSR +FYWK+E
Sbjct: 623 IISGKGLDEFKSEIAVLTRVRHRNLVVLHGYCLEGNERLLVYQYMPQGTLSRHIFYWKEE 682

Query: 694 GINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHRDLKPSNILLGDDMRAKVADFGLVRL 753
           G+ PLEW RRLI+ALDVARGVEYLH+LAHQSFIHRDLKPSNILLGDDM AKVADFGLVRL
Sbjct: 683 GLRPLEWTRRLIIALDVARGVEYLHTLAHQSFIHRDLKPSNILLGDDMHAKVADFGLVRL 742

Query: 754 APEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMEMITGRKALDDSQPEEC 813
           APEG  S+ET++AGTFGYLAPEYAVTGRVTTKVDVYSFGVILME++TGRKALD ++ EE 
Sbjct: 743 APEGTQSIETKIAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMELLTGRKALDVARSEEE 802

Query: 814 LHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSISTVADLAGHCCAREPYQRPDMSHAV 873
           +HL +WFRRM INK +FPKAID ++ +++ETL SI+ VA+LA  C +REP  RPDM+H V
Sbjct: 803 VHLATWFRRMFINKGSFPKAIDEAMEVNEETLRSINIVAELANQCSSREPRDRPDMNHVV 862

Query: 874 NVLSSLIDVWKPTE--PDSEEVLGIDLEMSLPQALKKWQAFEGNSNMDASSSSSSFLASG 933
           NVL SL+  WKPTE   DSE++ GID +  LPQ +                  S F   G
Sbjct: 863 NVLVSLVVQWKPTERSSDSEDIYGIDYDTPLPQLI----------------LDSCFF--G 886

Query: 934 DNTQTSIPNRPSGFANSFTSVDAR 948
           DNT TSIP+RPS   ++F S   R
Sbjct: 923 DNTLTSIPSRPSELESTFKSGQGR 886

BLAST of MC04g_new0454 vs. ExPASy Swiss-Prot
Match: Q9LK43 (Receptor-like kinase TMK4 OS=Arabidopsis thaliana OX=3702 GN=TMK4 PE=1 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 6.1e-233
Identity = 451/926 (48.70%), Postives = 598/926 (64.58%), Query Frame = 0

Query: 34  DGSVMVILRTSIGAPASLGWSGSDYCQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSL 93
           D + M+ L  S   P S   S +D+C+W  V+C +  RV  I + +++L G +  E+ +L
Sbjct: 26  DQTAMLALAKSFNPPPSDWSSTTDFCKWSGVRC-TGGRVTTISLADKSLTGFIAPEISTL 85

Query: 94  SALVQLEVQGNQLSGPFPNLAE--SLQILLAHDNLFTSLPADFFAKKSTLQMIEI-DNNP 153
           S L  + +Q N+LSG  P+ A+  SLQ +   +N F  +    FA  ++LQ++ + DNN 
Sbjct: 86  SELKSVSIQRNKLSGTIPSFAKLSSLQEIYMDENNFVGVETGAFAGLTSLQILSLSDNNN 145

Query: 154 FSAWKIPDNIRDASGLQEISANRVNITGTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASF 213
            + W  P  + D++ L  I  +  NI G +PD FD  +  +L+NL LS N + G LP S 
Sbjct: 146 ITTWSFPSELVDSTSLTTIYLDNTNIAGVLPDIFD--SLASLQNLRLSYNNITGVLPPSL 205

Query: 214 SGSSIQSLWLNGQQSNFKLNGSVAILQNMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSL 273
             SSIQ+LW+N Q  +  ++G++ +L +MT+L + WLH N F GP+PD S  + L +L L
Sbjct: 206 GKSSIQNLWINNQ--DLGMSGTIEVLSSMTSLSQAWLHKNHFFGPIPDLSKSENLFDLQL 265

Query: 274 RDNQLTGIVPPSLVNLKSLVIVNLTNNMLQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPC 333
           RDN LTGIVPP+L+ L SL  ++L NN  QGP P+F   V++ +    N FC    G  C
Sbjct: 266 RDNDLTGIVPPTLLTLASLKNISLDNNKFQGPLPLFSPEVKVTI--DHNVFCTTKAGQSC 325

Query: 334 DPRVNALLSVAQSMNFPTVFAKNWKGNDPCNFKGVVCIGNSPVNVTVLNFRNLGLAGTIS 393
            P+V  LL+VA  + +P++ A++W+G+D C+    V   ++  NV  LN    G  G IS
Sbjct: 326 SPQVMTLLAVAGGLGYPSMLAESWQGDDACSGWAYVSCDSAGKNVVTLNLGKHGFTGFIS 385

Query: 394 PSFSLLTSVTKLLLSDNLLSGVIPTELVTMPSLVELDVSNNRLHGKVPSFRKNVIVN-TD 453
           P+ + LTS+  L L+ N L+GVIP EL  M SL  +DVSNN L G++P F   V  +   
Sbjct: 386 PAIANLTSLKSLYLNGNDLTGVIPKELTFMTSLQLIDVSNNNLRGEIPKFPATVKFSYKP 445

Query: 454 GNPDIGKDRRSTPPPGSPTEKSPSDPGSGGLPVDVDKKSNSGVVVGSIIGV-IGLVLVGA 513
           GN  +G +      PG+          SGG        S  GV+VG I+ V + L ++G 
Sbjct: 446 GNALLGTNGGDGSSPGTGGASGGPGGSSGG------GGSKVGVIVGVIVAVLVFLAILGF 505

Query: 514 VIF-FLYRRNKRRSSRV--QSPNTVVVHP--SHSGDQNAVKITIAESRADGNAPETGRVP 573
           V++ F+ +R   R +R   +    ++V    S+ G  N      A      N        
Sbjct: 506 VVYKFVMKRKYGRFNRTDPEKVGKILVSDAVSNGGSGNG---GYANGHGANNFNALNSPS 565

Query: 574 IGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGSGGFGTVYKGELHDGTKIAVKRME 633
            G  SD  ++E G++ I ++VLR VTNNFS +NILG GGFG VY GELHDGTK AVKRME
Sbjct: 566 SGDNSDRFLLEGGSVTIPMEVLRQVTNNFSEDNILGRGGFGVVYAGELHDGTKTAVKRME 625

Query: 634 SGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDGNERLLVYEYMPQGTLSRFLFYWK 693
              +G KG++EF+AEIAVLTKVRHR+LVALLGYC++GNERLLVYEYMPQG L + LF W 
Sbjct: 626 CAAMGNKGMSEFQAEIAVLTKVRHRHLVALLGYCVNGNERLLVYEYMPQGNLGQHLFEWS 685

Query: 694 KEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHRDLKPSNILLGDDMRAKVADFGLV 753
           + G +PL WK+R+ +ALDVARGVEYLHSLA QSFIHRDLKPSNILLGDDMRAKVADFGLV
Sbjct: 686 ELGYSPLTWKQRVSIALDVARGVEYLHSLAQQSFIHRDLKPSNILLGDDMRAKVADFGLV 745

Query: 754 RLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMEMITGRKALDDSQPE 813
           + AP+GK SVETRLAGTFGYLAPEYA TGRVTTKVDVY+FGV+LME++TGRKALDDS P+
Sbjct: 746 KNAPDGKYSVETRLAGTFGYLAPEYAATGRVTTKVDVYAFGVVLMEILTGRKALDDSLPD 805

Query: 814 ECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSISTVADLAGHCCAREPYQRPDMSH 873
           E  HLV+WFRR+ INK+  PKA+D ++  D+ET+ SI  VA+LAGHC AREP QRPDM H
Sbjct: 806 ERSHLVTWFRRILINKENIPKALDQTLEADEETMESIYRVAELAGHCTAREPQQRPDMGH 865

Query: 874 AVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKKWQAFEGNSNMDASSSSSSFLA-- 933
           AVNVL  L++ WKP+  + EE  GID+ MSLPQAL++WQ        + +SSS+ F    
Sbjct: 866 AVNVLGPLVEKWKPSCQEEEESFGIDVNMSLPQALQRWQ-------NEGTSSSTMFHGDF 925

Query: 934 SGDNTQTSIPNRPSGFANSFTSVDAR 948
           S   TQ+SIP + SGF N+F S D R
Sbjct: 926 SYSQTQSSIPPKASGFPNTFDSADGR 928

BLAST of MC04g_new0454 vs. ExPASy Swiss-Prot
Match: Q658G7 (LRR receptor-like serine/threonine-protein kinase SIK1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIK1 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.7e-84
Identity = 266/962 (27.65%), Postives = 427/962 (44.39%), Query Frame = 0

Query: 30  GGDGDGSVMVILRTSIG--APASLGW-SGSDYCQWKHVKCDSEN-RVVKIQIGNQN---- 89
           GGDG+G  ++ ++   G  A A + W  G+D+C W+ V CD+ +  V+ + + N N    
Sbjct: 31  GGDGEGKALMGVKAGFGNAANALVDWDGGADHCAWRGVTCDNASFAVLALNLSNLNLGGE 90

Query: 90  --------------------LKGSLPSEL---------------------FSLSALVQLE 149
                               L G +P E+                     FS+S L QLE
Sbjct: 91  ISPAIGELKNLQFVDLKGNKLTGQIPDEIGDCISLKYLDLSGNLLYGDIPFSISKLKQLE 150

Query: 150 ---VQGNQLSGPFPNLAESLQIL----LAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSA 209
              ++ NQL+GP P+    +  L    LA + L   +P   +  +  LQ + +  N  + 
Sbjct: 151 ELILKNNQLTGPIPSTLSQIPNLKTLDLAQNQLTGDIPRLIYWNE-VLQYLGLRGNSLTG 210

Query: 210 WKIPDNIRDASGLQEISANRVNITGTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGS 269
              PD +   +GL        N+TGTIP+     T  +   L +S N + G +P +    
Sbjct: 211 TLSPD-MCQLTGLWYFDVRGNNLTGTIPESIGNCT--SFEILDISYNQISGEIPYNIGFL 270

Query: 270 SIQSLWLNGQQSNFKLNGSVAILQ---------------------NMTNLQEIWLHTNQF 329
            + +L L G +   K+   + ++Q                     N++   +++LH N+ 
Sbjct: 271 QVATLSLQGNRLTGKIPDVIGLMQALAVLDLSENELVGPIPSILGNLSYTGKLYLHGNKL 330

Query: 330 SGPL-PDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQGPAPVFDRNVQ 389
           +G + P+  N+  L  L L DN+L G +P  L  L+ L  +NL NN LQGP P       
Sbjct: 331 TGVIPPELGNMSKLSYLQLNDNELVGTIPAELGKLEELFELNLANNNLQGPIP-----AN 390

Query: 390 LDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPCNFKGVVCIG-- 449
           +      N F V   G   +  + A     +S+ +  + + N+KGN P     ++ +   
Sbjct: 391 ISSCTALNKFNV--YGNKLNGSIPAGFQKLESLTYLNLSSNNFKGNIPSELGHIINLDTL 450

Query: 450 -------NSPVNVTV--------LNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIP 509
                  + PV  T+        LN     L G +   F  L SV  + +S+N LSG +P
Sbjct: 451 DLSYNEFSGPVPATIGDLEHLLELNLSKNHLDGPVPAEFGNLRSVQVIDMSNNNLSGSLP 510

Query: 510 TELVTMPSLVELDVSNNRLHGKVPSFRKNVIV---------NTDGNPDIGKDRRSTPPP- 569
            EL  + +L  L ++NN L G++P+   N            N  G+  + K+    P   
Sbjct: 511 EELGQLQNLDSLILNNNNLVGEIPAQLANCFSLNNLNLSYNNLSGHVPMAKNFSKFPMES 570

Query: 570 --GSPTE----KSPSDPGSGGLPVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNK 629
             G+P      +  S   S G  V++ K + + +++G II      L+  ++  +Y+ N+
Sbjct: 571 FLGNPLLHVYCQDSSCGHSHGQRVNISKTAIACIILGFII------LLCVLLLAIYKTNQ 630

Query: 630 RRSSRVQSPNTVVVHPSHSGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNM 689
            +             P   G                        P+ GP  + V++    
Sbjct: 631 PQ-------------PLVKGSDK---------------------PVQGPPKLVVLQMDMA 690

Query: 690 VISIQVLRNVTNNFSPENILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAE 749
           + + + +  +T N S + I+G G   TVYK EL  G  IAVKR+ S       L EF+ E
Sbjct: 691 IHTYEDIMRLTENLSEKYIIGYGASSTVYKCELKSGKAIAVKRLYSQY--NHSLREFETE 750

Query: 750 IAVLTKVRHRNLVALLGYCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIV 809
           +  +  +RHRNLV+L G+ L  +  LL Y+YM  G+L   L    K+    L W  RL +
Sbjct: 751 LETIGSIRHRNLVSLHGFSLSPHGNLLFYDYMENGSLWDLLHGPSKK--VKLNWDTRLRI 810

Query: 810 ALDVARGVEYLHSLAHQSFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLA 869
           A+  A+G+ YLH   +   IHRD+K SNILL ++  A ++DFG+ +  P  K+   T + 
Sbjct: 811 AVGAAQGLAYLHHDCNPRIIHRDVKSSNILLDENFEAHLSDFGIAKCVPSAKSHASTYVL 870

Query: 870 GTFGYLAPEYAVTGRVTTKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHIN 881
           GT GY+ PEYA T R+  K DVYSFG++L+E++TG+KA+D+   E  LH +   +    +
Sbjct: 871 GTIGYIDPEYARTSRLNEKSDVYSFGIVLLELLTGKKAVDN---ESNLHQLILSK---AD 930

BLAST of MC04g_new0454 vs. NCBI nr
Match: XP_022146358.1 (receptor protein kinase TMK1-like [Momordica charantia])

HSP 1 Score: 1888 bits (4891), Expect = 0.0
Identity = 949/949 (100.00%), Postives = 949/949 (100.00%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ 60
           MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ
Sbjct: 1   MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ 60

Query: 61  WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQIL 120
           WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQIL
Sbjct: 61  WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQIL 120

Query: 121 LAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTI 180
           LAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTI
Sbjct: 121 LAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTI 180

Query: 181 PDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMT 240
           PDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMT
Sbjct: 181 PDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMT 240

Query: 241 NLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQ 300
           NLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQ
Sbjct: 241 NLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQ 300

Query: 301 GPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC 360
           GPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC
Sbjct: 301 GPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC 360

Query: 361 NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTM 420
           NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTM
Sbjct: 361 NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTM 420

Query: 421 PSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGL 480
           PSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGL
Sbjct: 421 PSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGL 480

Query: 481 PVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSGDQ 540
           PVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSGDQ
Sbjct: 481 PVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSGDQ 540

Query: 541 NAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGS 600
           NAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGS
Sbjct: 541 NAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGS 600

Query: 601 GGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDG 660
           GGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDG
Sbjct: 601 GGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDG 660

Query: 661 NERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHR 720
           NERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHR
Sbjct: 661 NERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHR 720

Query: 721 DLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDV 780
           DLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDV
Sbjct: 721 DLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDV 780

Query: 781 YSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSI 840
           YSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSI
Sbjct: 781 YSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSI 840

Query: 841 STVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKK 900
           STVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKK
Sbjct: 841 STVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKK 900

Query: 901 WQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDARTR 949
           WQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDARTR
Sbjct: 901 WQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDARTR 949

BLAST of MC04g_new0454 vs. NCBI nr
Match: XP_038874365.1 (receptor protein kinase TMK1-like [Benincasa hispida])

HSP 1 Score: 1597 bits (4135), Expect = 0.0
Identity = 806/951 (84.75%), Postives = 868/951 (91.27%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDY 60
           MREPH G GVFL V   FL F  + V+AQ +GG GDGSVM +L+ SI AP+SLGW+GSDY
Sbjct: 1   MREPHLGFGVFLLVFPLFLQFPGFSVFAQTAGGGGDGSVMNLLKNSIAAPSSLGWTGSDY 60

Query: 61  CQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQ 120
           CQWKHVKCDS+NRVVKIQIGNQNLKGSLP+EL SLSALVQLEVQ NQL GPFPNLA+SLQ
Sbjct: 61  CQWKHVKCDSQNRVVKIQIGNQNLKGSLPTELSSLSALVQLEVQSNQLGGPFPNLADSLQ 120

Query: 121 ILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITG 180
           ILLAHDNLFTS+P DFF KKSTLQ I IDNNPFSAW+IPDNIRDASGLQE+SANRVNITG
Sbjct: 121 ILLAHDNLFTSMPDDFFVKKSTLQTIVIDNNPFSAWQIPDNIRDASGLQELSANRVNITG 180

Query: 181 TIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQN 240
            IP+ FDG TFPTLRNLHL+GNFLEGGLP S +GSSIQSLWLNGQQS+ KLNGS+ ILQN
Sbjct: 181 KIPEVFDGATFPTLRNLHLAGNFLEGGLPESLAGSSIQSLWLNGQQSSSKLNGSIDILQN 240

Query: 241 MTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNM 300
           MTNLQEIWLH NQFSGPLPDFSNLQGL +LSLRDNQLTGIVP SLVNLKSL++VNLTNNM
Sbjct: 241 MTNLQEIWLHLNQFSGPLPDFSNLQGLVHLSLRDNQLTGIVPLSLVNLKSLMVVNLTNNM 300

Query: 301 LQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGND 360
           LQGP PVF  NV+LDMRP +N FC+DSPG PCDPRVNALLSVAQSM FPTVFA+ W+GN+
Sbjct: 301 LQGPTPVFSPNVKLDMRPASNKFCLDSPGKPCDPRVNALLSVAQSMGFPTVFAQGWEGNN 360

Query: 361 PC-NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTEL 420
           PC NFKGV C GN P N+T++NF+N+GLAG+IS SFSLLTSV KL LS+N LSG IP EL
Sbjct: 361 PCENFKGVECTGN-PGNITIINFKNMGLAGSISSSFSLLTSVQKLFLSNNFLSGTIPNEL 420

Query: 421 VTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGS 480
           VTMPSL+ELDVSNNRLHGK+P+FRKNVIV+T GNPDIGKD  S P PGSPT KSPSD GS
Sbjct: 421 VTMPSLIELDVSNNRLHGKIPAFRKNVIVHTAGNPDIGKDTASAPIPGSPTGKSPSD-GS 480

Query: 481 GGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSH 540
           G +  +  KKSN+GVVVGSIIGVI GL++VG VIFFLY+R KR  SRVQSPNTVVVHPSH
Sbjct: 481 GDISDNGVKKSNTGVVVGSIIGVIIGLLVVGIVIFFLYKRKKRHGSRVQSPNTVVVHPSH 540

Query: 541 SGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPEN 600
           SGDQN+VKITI E+RADG+APET RVPI GPSDVHVVEAGN+VISIQVLRNVTNNFSPEN
Sbjct: 541 SGDQNSVKITITETRADGSAPETSRVPIAGPSDVHVVEAGNLVISIQVLRNVTNNFSPEN 600

Query: 601 ILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGY 660
           ILG GGFGTVYKGELHDGT IAVKRMESGVIG KGLNEFKAEIAVLTKVRHRNLVALLGY
Sbjct: 601 ILGKGGFGTVYKGELHDGTNIAVKRMESGVIGGKGLNEFKAEIAVLTKVRHRNLVALLGY 660

Query: 661 CLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQS 720
           CLDGNERLLVYEYMPQGT SRFLF+WK+EGI PLEWKRRLIV LDVARGVEYLHSLAHQS
Sbjct: 661 CLDGNERLLVYEYMPQGTFSRFLFHWKEEGIKPLEWKRRLIVVLDVARGVEYLHSLAHQS 720

Query: 721 FIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTT 780
           FIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKAS+ETRLAGTFGYLAPEYAVTGRVTT
Sbjct: 721 FIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASIETRLAGTFGYLAPEYAVTGRVTT 780

Query: 781 KVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQET 840
           KVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRMHINKDTF KAIDPSI +D+ET
Sbjct: 781 KVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMHINKDTFSKAIDPSIDVDEET 840

Query: 841 LGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQ 900
             SI+TVADLAGHCCAREPYQRPDMSHAVNVLSSL+DVWKPTEPDSEE LGIDLE+SLPQ
Sbjct: 841 FSSINTVADLAGHCCAREPYQRPDMSHAVNVLSSLVDVWKPTEPDSEENLGIDLELSLPQ 900

Query: 901 ALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           AL+KWQAFEGNSN+DASSSSSSFLASGDNTQTSIPNRPSGFA+SFTSVDAR
Sbjct: 901 ALRKWQAFEGNSNIDASSSSSSFLASGDNTQTSIPNRPSGFADSFTSVDAR 949

BLAST of MC04g_new0454 vs. NCBI nr
Match: XP_008467095.1 (PREDICTED: receptor protein kinase TMK1 [Cucumis melo])

HSP 1 Score: 1585 bits (4104), Expect = 0.0
Identity = 802/951 (84.33%), Postives = 861/951 (90.54%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDY 60
           MR+PH G GVFL V   FL F  + V+AQP+ G GDGSVM +L+ SIGAP+SLGW+GSDY
Sbjct: 1   MRKPHLGFGVFLLVFPLFLQFLGFSVFAQPAAG-GDGSVMSLLKNSIGAPSSLGWTGSDY 60

Query: 61  CQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQ 120
           CQWKHVKCDS++RVVKIQIGNQNLKGSLP ELFSLSALVQLEVQ NQL GPFPNLA+SLQ
Sbjct: 61  CQWKHVKCDSQSRVVKIQIGNQNLKGSLPKELFSLSALVQLEVQSNQLGGPFPNLADSLQ 120

Query: 121 ILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITG 180
           ILLAHDN FTS+PADFFAKKS LQ I+IDNNPFSAW+IPDNIRDASGLQEIS NRVNITG
Sbjct: 121 ILLAHDNFFTSMPADFFAKKSALQTIDIDNNPFSAWQIPDNIRDASGLQEISFNRVNITG 180

Query: 181 TIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQN 240
           TIP+ FDG TFPTLRNLHL+GNFLEG LPAS +GSSIQSLWLNGQQS+ KLNGS+AILQN
Sbjct: 181 TIPEIFDGATFPTLRNLHLAGNFLEGELPASLAGSSIQSLWLNGQQSSSKLNGSIAILQN 240

Query: 241 MTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNM 300
           MTNLQE+WLH NQFSGPLPDFSNLQGL  LSLRDNQLTGIVP SLVNLKSL++VNLTNN+
Sbjct: 241 MTNLQEVWLHLNQFSGPLPDFSNLQGLAKLSLRDNQLTGIVPSSLVNLKSLMVVNLTNNL 300

Query: 301 LQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGND 360
           LQGP P FD NVQLDMRP TN FC+DSPG PCDPRVNALLSVA++M FPT FA+ W GND
Sbjct: 301 LQGPTPAFDPNVQLDMRPQTNKFCLDSPGKPCDPRVNALLSVAEAMGFPTAFAQGWGGND 360

Query: 361 PCN-FKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTEL 420
           PC  FKG+ CIG +P N+TV+NF+N+GLAG+ISPSFSLLTSV KL LSDN LSG IP EL
Sbjct: 361 PCQGFKGISCIG-TPTNITVINFKNMGLAGSISPSFSLLTSVQKLFLSDNFLSGTIPNEL 420

Query: 421 VTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGS 480
            TMPSL ELDVSNNRLHGKVP+FRKNVIVNT GNPDIGKD  S P PGSPT +SPSD GS
Sbjct: 421 ATMPSLTELDVSNNRLHGKVPAFRKNVIVNTQGNPDIGKDNPSAPVPGSPTRRSPSD-GS 480

Query: 481 GGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSH 540
           G    + +KKSN+GVVVGSIIGVI GL++VG VIFFL +R KR  SRVQSPNTVVVHPSH
Sbjct: 481 GDSAGNDEKKSNTGVVVGSIIGVIVGLLVVGTVIFFLCKRKKRHGSRVQSPNTVVVHPSH 540

Query: 541 SGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPEN 600
           SGDQN+VKITI E+R+DG+  ET RVPI GPSDVHVVEAGN+VISIQVLR+VTNNFSPEN
Sbjct: 541 SGDQNSVKITITEARSDGSVAETSRVPIAGPSDVHVVEAGNLVISIQVLRSVTNNFSPEN 600

Query: 601 ILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGY 660
           ILG GGFGTVYKGELHDGT IAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGY
Sbjct: 601 ILGKGGFGTVYKGELHDGTMIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGY 660

Query: 661 CLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQS 720
           CLDGNERLLVYEYMPQGT SRFLF WK+EGI PLEWKRRL V LDVARGVEYLHSLAHQS
Sbjct: 661 CLDGNERLLVYEYMPQGTFSRFLFNWKEEGIKPLEWKRRLTVVLDVARGVEYLHSLAHQS 720

Query: 721 FIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTT 780
           FIHRDLKPSNILLGDD+RAKVADFGLVRLAPEGKAS+ETRLAGTFGYLAPEYAVTGRVTT
Sbjct: 721 FIHRDLKPSNILLGDDLRAKVADFGLVRLAPEGKASIETRLAGTFGYLAPEYAVTGRVTT 780

Query: 781 KVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQET 840
           KVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRMHINKDTF KAIDPSI LD+ET
Sbjct: 781 KVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMHINKDTFSKAIDPSIDLDEET 840

Query: 841 LGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQ 900
           L SI TVADLAGHCCAREPYQRPDMSHAVNVLSSL+DVWKPTEPDSEE LGIDLE+SLPQ
Sbjct: 841 LVSICTVADLAGHCCAREPYQRPDMSHAVNVLSSLVDVWKPTEPDSEENLGIDLELSLPQ 900

Query: 901 ALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           AL+KWQAFEGNSN+D SSSSS FLASGDNTQTSIPNRPSGFANSFTSVDAR
Sbjct: 901 ALRKWQAFEGNSNVDMSSSSS-FLASGDNTQTSIPNRPSGFANSFTSVDAR 947

BLAST of MC04g_new0454 vs. NCBI nr
Match: XP_004142822.1 (receptor protein kinase TMK1 [Cucumis sativus] >KGN51453.1 hypothetical protein Csa_008106 [Cucumis sativus])

HSP 1 Score: 1584 bits (4102), Expect = 0.0
Identity = 797/952 (83.72%), Postives = 863/952 (90.65%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPS-GGDGDGSVMVILRTSIGAPASLGWSGSD 60
           MR+PHFG GVFL +   FL F  + V+AQP+ GG GDGSVM +L+ S+GAP+SLGW+GSD
Sbjct: 1   MRKPHFGFGVFLLLFPLFLHFLGFSVFAQPAAGGAGDGSVMNLLKNSVGAPSSLGWTGSD 60

Query: 61  YCQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESL 120
           YCQW HVKCDS++RVVKIQIGNQNLKGSLP ELFSLSALVQLEVQ NQL GPFPNLA+SL
Sbjct: 61  YCQWNHVKCDSQSRVVKIQIGNQNLKGSLPKELFSLSALVQLEVQSNQLGGPFPNLADSL 120

Query: 121 QILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNIT 180
           QILLAHDNLFTS+PADFFAKKS LQ I+IDNNPFSAW+IPDNIRDAS LQ++SANRVNIT
Sbjct: 121 QILLAHDNLFTSMPADFFAKKSALQTIDIDNNPFSAWQIPDNIRDASALQQLSANRVNIT 180

Query: 181 GTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQ 240
           G IP  FDG TFPTL NLHL+GNFLEG LPAS +GSSIQSLWLNGQQS+ KLNGS+AILQ
Sbjct: 181 GIIPGIFDGATFPTLTNLHLAGNFLEGELPASLAGSSIQSLWLNGQQSSSKLNGSIAILQ 240

Query: 241 NMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNN 300
           NMTNLQE+WLH NQFSGPLPDFSNLQGL  LSLRDNQLTGIVP SLVNLKSL++VNLTNN
Sbjct: 241 NMTNLQEVWLHMNQFSGPLPDFSNLQGLAKLSLRDNQLTGIVPSSLVNLKSLMVVNLTNN 300

Query: 301 MLQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGN 360
           +LQGP P FD NVQLDM+P TN FC+DSPG PCDPRVNALLSVA+SM FPT FA+ W GN
Sbjct: 301 LLQGPTPAFDPNVQLDMKPQTNKFCLDSPGEPCDPRVNALLSVAESMGFPTAFAQGWAGN 360

Query: 361 DPCN-FKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTE 420
           DPC  FKG+ CIGN P N++V+N +N+GLAG+ISPSFSLLTSV KL LS+N LSG IP E
Sbjct: 361 DPCQGFKGISCIGN-PTNISVINLKNMGLAGSISPSFSLLTSVQKLFLSNNFLSGTIPNE 420

Query: 421 LVTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPG 480
           L TMPSL ELDVSNNRLHGKVP FRKNVIVNT GNPDIGKD  S P PGSPT +SPSD G
Sbjct: 421 LATMPSLTELDVSNNRLHGKVPVFRKNVIVNTQGNPDIGKDNASPPVPGSPTGRSPSD-G 480

Query: 481 SGGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPS 540
           SG    + +KKSN+GVVVG+IIGVI GL++VG VIFFL +R KRR +RVQSPNTVVVHPS
Sbjct: 481 SGDSAGNDEKKSNAGVVVGAIIGVIVGLLVVGTVIFFLCKRKKRRGNRVQSPNTVVVHPS 540

Query: 541 HSGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPE 600
           HSGDQN+VKITI E+R+DG+APET RVPI GPSDVHVVEAGN+VISIQVLR+VTNNFSPE
Sbjct: 541 HSGDQNSVKITITEARSDGSAPETSRVPIAGPSDVHVVEAGNLVISIQVLRSVTNNFSPE 600

Query: 601 NILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG 660
           NILG GGFGTVYKGELHDGT IAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG
Sbjct: 601 NILGKGGFGTVYKGELHDGTMIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG 660

Query: 661 YCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQ 720
           YCLDGNERLLVYEYMPQGT SRFLF WK+EGI PLEWKRRLIV LDVARGVEYLHSLAHQ
Sbjct: 661 YCLDGNERLLVYEYMPQGTFSRFLFNWKEEGIRPLEWKRRLIVVLDVARGVEYLHSLAHQ 720

Query: 721 SFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780
           SFIHRDLKPSNILLGDD+RAKVADFGLVRLAPEGKAS+ETRLAGTFGYLAPEYAVTGRVT
Sbjct: 721 SFIHRDLKPSNILLGDDLRAKVADFGLVRLAPEGKASIETRLAGTFGYLAPEYAVTGRVT 780

Query: 781 TKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQE 840
           TKVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRMHINKDTF KAIDPSI +D+E
Sbjct: 781 TKVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMHINKDTFSKAIDPSIDIDEE 840

Query: 841 TLGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLP 900
           TL SI+TVADLAGHCCAREPYQRPDM HAVNVLSSL+DVWKPTEPDSEE LGIDLE+SLP
Sbjct: 841 TLVSINTVADLAGHCCAREPYQRPDMGHAVNVLSSLVDVWKPTEPDSEENLGIDLELSLP 900

Query: 901 QALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           QAL+KWQAFEGNSN+D SSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR
Sbjct: 901 QALRKWQAFEGNSNVDMSSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 950

BLAST of MC04g_new0454 vs. NCBI nr
Match: XP_022939245.1 (receptor protein kinase TMK1-like [Cucurbita moschata])

HSP 1 Score: 1578 bits (4086), Expect = 0.0
Identity = 784/952 (82.35%), Postives = 865/952 (90.86%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPSGGDGD-GSVMVILRTSIGAPASLGWSGSD 60
           MREPH G GVFL +   FL F  + V+AQP+G  GD GSVM +L+TSIGAP SLGW+GS+
Sbjct: 1   MREPHLGFGVFLLIFVFFLLFLDFSVYAQPAGAGGDDGSVMNLLKTSIGAPDSLGWTGSN 60

Query: 61  YCQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESL 120
           YC+WKHVKCDS+NRVVKIQIGNQNLKGSLPS+LFSLSALVQLEVQ NQL+GPFPNLA+SL
Sbjct: 61  YCEWKHVKCDSQNRVVKIQIGNQNLKGSLPSDLFSLSALVQLEVQSNQLTGPFPNLADSL 120

Query: 121 QILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNIT 180
           QILLAHDNLFTS+PADFFAKKS LQ I++DNNPF AW+IPD+IRDASGLQE+SANR N+T
Sbjct: 121 QILLAHDNLFTSMPADFFAKKSALQTIDVDNNPFMAWQIPDSIRDASGLQELSANRANVT 180

Query: 181 GTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQ 240
           G     FDGTTFPTLRNLHL+GNFLEGGLP SF+GSSIQSLWLNGQQSN KLNGS+A+LQ
Sbjct: 181 GNTLQIFDGTTFPTLRNLHLAGNFLEGGLPVSFAGSSIQSLWLNGQQSNSKLNGSIAVLQ 240

Query: 241 NMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNN 300
           NMTNLQE+WLH N FSGPLPDFSNLQGL+ L+LRDNQLTGIVP SL+NLKSL +VNLTNN
Sbjct: 241 NMTNLQEVWLHMNHFSGPLPDFSNLQGLETLNLRDNQLTGIVPSSLINLKSLTVVNLTNN 300

Query: 301 MLQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGN 360
           +LQGP P F+ NV+LDMRPG+NNFC DSPG PCDPRV+ALLSVAQSM FPTVFA++W GN
Sbjct: 301 LLQGPTPAFNPNVKLDMRPGSNNFCTDSPGEPCDPRVSALLSVAQSMGFPTVFAQSWVGN 360

Query: 361 DPC-NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTE 420
           +PC +FKGV C GN P N+T++NF+N+GLAG+ISPSFSLLTSV KL+LS+N LSG IPTE
Sbjct: 361 NPCEDFKGVTCTGN-PENITIINFKNMGLAGSISPSFSLLTSVQKLILSNNYLSGTIPTE 420

Query: 421 LVTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPG 480
           LVTMPSL+ELDVS+NRLHGKVP+FRKNVIV T GNPDIGKDR S+P PGSP E +PSD G
Sbjct: 421 LVTMPSLIELDVSSNRLHGKVPAFRKNVIVTTQGNPDIGKDRPSSPAPGSPEEGAPSDYG 480

Query: 481 SGGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPS 540
           +G +P    KKSN+GVVVGSIIGVI GL+LVG  IFF Y+R KR  S VQ+PNTVVVHPS
Sbjct: 481 TGDVPDGGVKKSNTGVVVGSIIGVIVGLLLVGTAIFFWYKRKKRHGSIVQNPNTVVVHPS 540

Query: 541 HSGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPE 600
           HSGDQN+VKIT+ E+RADG+ PE+  VPI GPSDV VVEAGN+VISIQVLRNVTNNFSP 
Sbjct: 541 HSGDQNSVKITVTEARADGSTPESSGVPIAGPSDVRVVEAGNLVISIQVLRNVTNNFSPS 600

Query: 601 NILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG 660
           NILG GGFGTVYKGELHDGTKIAVKRMESGV+GEKGLNEFKAEIAVLTKVRHRNLVALLG
Sbjct: 601 NILGRGGFGTVYKGELHDGTKIAVKRMESGVVGEKGLNEFKAEIAVLTKVRHRNLVALLG 660

Query: 661 YCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQ 720
           YCLDGNERLLVYEYMPQGT SRFLF+W  EGI PLEWKRRL VALDVARGVEYLHSLAHQ
Sbjct: 661 YCLDGNERLLVYEYMPQGTFSRFLFHWNDEGIKPLEWKRRLTVALDVARGVEYLHSLAHQ 720

Query: 721 SFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780
           SFIHRDLKPSNILLGDD+RAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT
Sbjct: 721 SFIHRDLKPSNILLGDDLRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780

Query: 781 TKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQE 840
           TKVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRM+INKDTF KAIDPS+H+D+E
Sbjct: 781 TKVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMYINKDTFSKAIDPSMHIDEE 840

Query: 841 TLGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLP 900
           TLGSI TVADLAGHCCAREPYQRPDMSHAVNVLSSL+DVWKPTEPDSE+ LGIDLEMSLP
Sbjct: 841 TLGSICTVADLAGHCCAREPYQRPDMSHAVNVLSSLVDVWKPTEPDSEDTLGIDLEMSLP 900

Query: 901 QALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           QALK+WQAFEGNSN+D +SSSS+ LASGDNTQ+SIPNRP GFANSFTS+DAR
Sbjct: 901 QALKRWQAFEGNSNLDMTSSSSTLLASGDNTQSSIPNRPPGFANSFTSLDAR 951

BLAST of MC04g_new0454 vs. ExPASy TrEMBL
Match: A0A6J1CYD3 (receptor protein kinase TMK1-like OS=Momordica charantia OX=3673 GN=LOC111015589 PE=3 SV=1)

HSP 1 Score: 1888 bits (4891), Expect = 0.0
Identity = 949/949 (100.00%), Postives = 949/949 (100.00%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ 60
           MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ
Sbjct: 1   MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ 60

Query: 61  WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQIL 120
           WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQIL
Sbjct: 61  WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQIL 120

Query: 121 LAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTI 180
           LAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTI
Sbjct: 121 LAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTI 180

Query: 181 PDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMT 240
           PDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMT
Sbjct: 181 PDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMT 240

Query: 241 NLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQ 300
           NLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQ
Sbjct: 241 NLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQ 300

Query: 301 GPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC 360
           GPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC
Sbjct: 301 GPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC 360

Query: 361 NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTM 420
           NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTM
Sbjct: 361 NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTM 420

Query: 421 PSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGL 480
           PSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGL
Sbjct: 421 PSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGL 480

Query: 481 PVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSGDQ 540
           PVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSGDQ
Sbjct: 481 PVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSGDQ 540

Query: 541 NAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGS 600
           NAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGS
Sbjct: 541 NAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGS 600

Query: 601 GGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDG 660
           GGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDG
Sbjct: 601 GGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDG 660

Query: 661 NERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHR 720
           NERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHR
Sbjct: 661 NERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHR 720

Query: 721 DLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDV 780
           DLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDV
Sbjct: 721 DLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDV 780

Query: 781 YSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSI 840
           YSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSI
Sbjct: 781 YSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSI 840

Query: 841 STVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKK 900
           STVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKK
Sbjct: 841 STVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKK 900

Query: 901 WQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDARTR 949
           WQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDARTR
Sbjct: 901 WQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDARTR 949

BLAST of MC04g_new0454 vs. ExPASy TrEMBL
Match: A0A1S3CU07 (receptor protein kinase TMK1 OS=Cucumis melo OX=3656 GN=LOC103504530 PE=3 SV=1)

HSP 1 Score: 1585 bits (4104), Expect = 0.0
Identity = 802/951 (84.33%), Postives = 861/951 (90.54%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDY 60
           MR+PH G GVFL V   FL F  + V+AQP+ G GDGSVM +L+ SIGAP+SLGW+GSDY
Sbjct: 1   MRKPHLGFGVFLLVFPLFLQFLGFSVFAQPAAG-GDGSVMSLLKNSIGAPSSLGWTGSDY 60

Query: 61  CQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESLQ 120
           CQWKHVKCDS++RVVKIQIGNQNLKGSLP ELFSLSALVQLEVQ NQL GPFPNLA+SLQ
Sbjct: 61  CQWKHVKCDSQSRVVKIQIGNQNLKGSLPKELFSLSALVQLEVQSNQLGGPFPNLADSLQ 120

Query: 121 ILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITG 180
           ILLAHDN FTS+PADFFAKKS LQ I+IDNNPFSAW+IPDNIRDASGLQEIS NRVNITG
Sbjct: 121 ILLAHDNFFTSMPADFFAKKSALQTIDIDNNPFSAWQIPDNIRDASGLQEISFNRVNITG 180

Query: 181 TIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQN 240
           TIP+ FDG TFPTLRNLHL+GNFLEG LPAS +GSSIQSLWLNGQQS+ KLNGS+AILQN
Sbjct: 181 TIPEIFDGATFPTLRNLHLAGNFLEGELPASLAGSSIQSLWLNGQQSSSKLNGSIAILQN 240

Query: 241 MTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNM 300
           MTNLQE+WLH NQFSGPLPDFSNLQGL  LSLRDNQLTGIVP SLVNLKSL++VNLTNN+
Sbjct: 241 MTNLQEVWLHLNQFSGPLPDFSNLQGLAKLSLRDNQLTGIVPSSLVNLKSLMVVNLTNNL 300

Query: 301 LQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGND 360
           LQGP P FD NVQLDMRP TN FC+DSPG PCDPRVNALLSVA++M FPT FA+ W GND
Sbjct: 301 LQGPTPAFDPNVQLDMRPQTNKFCLDSPGKPCDPRVNALLSVAEAMGFPTAFAQGWGGND 360

Query: 361 PCN-FKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTEL 420
           PC  FKG+ CIG +P N+TV+NF+N+GLAG+ISPSFSLLTSV KL LSDN LSG IP EL
Sbjct: 361 PCQGFKGISCIG-TPTNITVINFKNMGLAGSISPSFSLLTSVQKLFLSDNFLSGTIPNEL 420

Query: 421 VTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGS 480
            TMPSL ELDVSNNRLHGKVP+FRKNVIVNT GNPDIGKD  S P PGSPT +SPSD GS
Sbjct: 421 ATMPSLTELDVSNNRLHGKVPAFRKNVIVNTQGNPDIGKDNPSAPVPGSPTRRSPSD-GS 480

Query: 481 GGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSH 540
           G    + +KKSN+GVVVGSIIGVI GL++VG VIFFL +R KR  SRVQSPNTVVVHPSH
Sbjct: 481 GDSAGNDEKKSNTGVVVGSIIGVIVGLLVVGTVIFFLCKRKKRHGSRVQSPNTVVVHPSH 540

Query: 541 SGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPEN 600
           SGDQN+VKITI E+R+DG+  ET RVPI GPSDVHVVEAGN+VISIQVLR+VTNNFSPEN
Sbjct: 541 SGDQNSVKITITEARSDGSVAETSRVPIAGPSDVHVVEAGNLVISIQVLRSVTNNFSPEN 600

Query: 601 ILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGY 660
           ILG GGFGTVYKGELHDGT IAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGY
Sbjct: 601 ILGKGGFGTVYKGELHDGTMIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGY 660

Query: 661 CLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQS 720
           CLDGNERLLVYEYMPQGT SRFLF WK+EGI PLEWKRRL V LDVARGVEYLHSLAHQS
Sbjct: 661 CLDGNERLLVYEYMPQGTFSRFLFNWKEEGIKPLEWKRRLTVVLDVARGVEYLHSLAHQS 720

Query: 721 FIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTT 780
           FIHRDLKPSNILLGDD+RAKVADFGLVRLAPEGKAS+ETRLAGTFGYLAPEYAVTGRVTT
Sbjct: 721 FIHRDLKPSNILLGDDLRAKVADFGLVRLAPEGKASIETRLAGTFGYLAPEYAVTGRVTT 780

Query: 781 KVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQET 840
           KVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRMHINKDTF KAIDPSI LD+ET
Sbjct: 781 KVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMHINKDTFSKAIDPSIDLDEET 840

Query: 841 LGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQ 900
           L SI TVADLAGHCCAREPYQRPDMSHAVNVLSSL+DVWKPTEPDSEE LGIDLE+SLPQ
Sbjct: 841 LVSICTVADLAGHCCAREPYQRPDMSHAVNVLSSLVDVWKPTEPDSEENLGIDLELSLPQ 900

Query: 901 ALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           AL+KWQAFEGNSN+D SSSSS FLASGDNTQTSIPNRPSGFANSFTSVDAR
Sbjct: 901 ALRKWQAFEGNSNVDMSSSSS-FLASGDNTQTSIPNRPSGFANSFTSVDAR 947

BLAST of MC04g_new0454 vs. ExPASy TrEMBL
Match: A0A0A0KPC4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G550170 PE=3 SV=1)

HSP 1 Score: 1584 bits (4102), Expect = 0.0
Identity = 797/952 (83.72%), Postives = 863/952 (90.65%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPS-GGDGDGSVMVILRTSIGAPASLGWSGSD 60
           MR+PHFG GVFL +   FL F  + V+AQP+ GG GDGSVM +L+ S+GAP+SLGW+GSD
Sbjct: 1   MRKPHFGFGVFLLLFPLFLHFLGFSVFAQPAAGGAGDGSVMNLLKNSVGAPSSLGWTGSD 60

Query: 61  YCQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESL 120
           YCQW HVKCDS++RVVKIQIGNQNLKGSLP ELFSLSALVQLEVQ NQL GPFPNLA+SL
Sbjct: 61  YCQWNHVKCDSQSRVVKIQIGNQNLKGSLPKELFSLSALVQLEVQSNQLGGPFPNLADSL 120

Query: 121 QILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNIT 180
           QILLAHDNLFTS+PADFFAKKS LQ I+IDNNPFSAW+IPDNIRDAS LQ++SANRVNIT
Sbjct: 121 QILLAHDNLFTSMPADFFAKKSALQTIDIDNNPFSAWQIPDNIRDASALQQLSANRVNIT 180

Query: 181 GTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQ 240
           G IP  FDG TFPTL NLHL+GNFLEG LPAS +GSSIQSLWLNGQQS+ KLNGS+AILQ
Sbjct: 181 GIIPGIFDGATFPTLTNLHLAGNFLEGELPASLAGSSIQSLWLNGQQSSSKLNGSIAILQ 240

Query: 241 NMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNN 300
           NMTNLQE+WLH NQFSGPLPDFSNLQGL  LSLRDNQLTGIVP SLVNLKSL++VNLTNN
Sbjct: 241 NMTNLQEVWLHMNQFSGPLPDFSNLQGLAKLSLRDNQLTGIVPSSLVNLKSLMVVNLTNN 300

Query: 301 MLQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGN 360
           +LQGP P FD NVQLDM+P TN FC+DSPG PCDPRVNALLSVA+SM FPT FA+ W GN
Sbjct: 301 LLQGPTPAFDPNVQLDMKPQTNKFCLDSPGEPCDPRVNALLSVAESMGFPTAFAQGWAGN 360

Query: 361 DPCN-FKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTE 420
           DPC  FKG+ CIGN P N++V+N +N+GLAG+ISPSFSLLTSV KL LS+N LSG IP E
Sbjct: 361 DPCQGFKGISCIGN-PTNISVINLKNMGLAGSISPSFSLLTSVQKLFLSNNFLSGTIPNE 420

Query: 421 LVTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPG 480
           L TMPSL ELDVSNNRLHGKVP FRKNVIVNT GNPDIGKD  S P PGSPT +SPSD G
Sbjct: 421 LATMPSLTELDVSNNRLHGKVPVFRKNVIVNTQGNPDIGKDNASPPVPGSPTGRSPSD-G 480

Query: 481 SGGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPS 540
           SG    + +KKSN+GVVVG+IIGVI GL++VG VIFFL +R KRR +RVQSPNTVVVHPS
Sbjct: 481 SGDSAGNDEKKSNAGVVVGAIIGVIVGLLVVGTVIFFLCKRKKRRGNRVQSPNTVVVHPS 540

Query: 541 HSGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPE 600
           HSGDQN+VKITI E+R+DG+APET RVPI GPSDVHVVEAGN+VISIQVLR+VTNNFSPE
Sbjct: 541 HSGDQNSVKITITEARSDGSAPETSRVPIAGPSDVHVVEAGNLVISIQVLRSVTNNFSPE 600

Query: 601 NILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG 660
           NILG GGFGTVYKGELHDGT IAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG
Sbjct: 601 NILGKGGFGTVYKGELHDGTMIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG 660

Query: 661 YCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQ 720
           YCLDGNERLLVYEYMPQGT SRFLF WK+EGI PLEWKRRLIV LDVARGVEYLHSLAHQ
Sbjct: 661 YCLDGNERLLVYEYMPQGTFSRFLFNWKEEGIRPLEWKRRLIVVLDVARGVEYLHSLAHQ 720

Query: 721 SFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780
           SFIHRDLKPSNILLGDD+RAKVADFGLVRLAPEGKAS+ETRLAGTFGYLAPEYAVTGRVT
Sbjct: 721 SFIHRDLKPSNILLGDDLRAKVADFGLVRLAPEGKASIETRLAGTFGYLAPEYAVTGRVT 780

Query: 781 TKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQE 840
           TKVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRMHINKDTF KAIDPSI +D+E
Sbjct: 781 TKVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMHINKDTFSKAIDPSIDIDEE 840

Query: 841 TLGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLP 900
           TL SI+TVADLAGHCCAREPYQRPDM HAVNVLSSL+DVWKPTEPDSEE LGIDLE+SLP
Sbjct: 841 TLVSINTVADLAGHCCAREPYQRPDMGHAVNVLSSLVDVWKPTEPDSEENLGIDLELSLP 900

Query: 901 QALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           QAL+KWQAFEGNSN+D SSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR
Sbjct: 901 QALRKWQAFEGNSNVDMSSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 950

BLAST of MC04g_new0454 vs. ExPASy TrEMBL
Match: A0A6J1FM55 (receptor protein kinase TMK1-like OS=Cucurbita moschata OX=3662 GN=LOC111445218 PE=3 SV=1)

HSP 1 Score: 1578 bits (4086), Expect = 0.0
Identity = 784/952 (82.35%), Postives = 865/952 (90.86%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPSGGDGD-GSVMVILRTSIGAPASLGWSGSD 60
           MREPH G GVFL +   FL F  + V+AQP+G  GD GSVM +L+TSIGAP SLGW+GS+
Sbjct: 1   MREPHLGFGVFLLIFVFFLLFLDFSVYAQPAGAGGDDGSVMNLLKTSIGAPDSLGWTGSN 60

Query: 61  YCQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESL 120
           YC+WKHVKCDS+NRVVKIQIGNQNLKGSLPS+LFSLSALVQLEVQ NQL+GPFPNLA+SL
Sbjct: 61  YCEWKHVKCDSQNRVVKIQIGNQNLKGSLPSDLFSLSALVQLEVQSNQLTGPFPNLADSL 120

Query: 121 QILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNIT 180
           QILLAHDNLFTS+PADFFAKKS LQ I++DNNPF AW+IPD+IRDASGLQE+SANR N+T
Sbjct: 121 QILLAHDNLFTSMPADFFAKKSALQTIDVDNNPFMAWQIPDSIRDASGLQELSANRANVT 180

Query: 181 GTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQ 240
           G     FDGTTFPTLRNLHL+GNFLEGGLP SF+GSSIQSLWLNGQQSN KLNGS+A+LQ
Sbjct: 181 GNTLQIFDGTTFPTLRNLHLAGNFLEGGLPVSFAGSSIQSLWLNGQQSNSKLNGSIAVLQ 240

Query: 241 NMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNN 300
           NMTNLQE+WLH N FSGPLPDFSNLQGL+ L+LRDNQLTGIVP SL+NLKSL +VNLTNN
Sbjct: 241 NMTNLQEVWLHMNHFSGPLPDFSNLQGLETLNLRDNQLTGIVPSSLINLKSLTVVNLTNN 300

Query: 301 MLQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGN 360
           +LQGP P F+ NV+LDMRPG+NNFC DSPG PCDPRV+ALLSVAQSM FPTVFA++W GN
Sbjct: 301 LLQGPTPAFNPNVKLDMRPGSNNFCTDSPGEPCDPRVSALLSVAQSMGFPTVFAQSWVGN 360

Query: 361 DPC-NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTE 420
           +PC +FKGV C GN P N+T++NF+N+GLAG+ISPSFSLLTSV KL+LS+N LSG IPTE
Sbjct: 361 NPCEDFKGVTCTGN-PENITIINFKNMGLAGSISPSFSLLTSVQKLILSNNYLSGTIPTE 420

Query: 421 LVTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPG 480
           LVTMPSL+ELDVS+NRLHGKVP+FRKNVIV T GNPDIGKDR S+P PGSP E +PSD G
Sbjct: 421 LVTMPSLIELDVSSNRLHGKVPAFRKNVIVTTQGNPDIGKDRPSSPAPGSPEEGAPSDYG 480

Query: 481 SGGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPS 540
           +G +P    KKSN+GVVVGSIIGVI GL+LVG  IFF Y+R KR  S VQ+PNTVVVHPS
Sbjct: 481 TGDVPDGGVKKSNTGVVVGSIIGVIVGLLLVGTAIFFWYKRKKRHGSIVQNPNTVVVHPS 540

Query: 541 HSGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPE 600
           HSGDQN+VKIT+ E+RADG+ PE+  VPI GPSDV VVEAGN+VISIQVLRNVTNNFSP 
Sbjct: 541 HSGDQNSVKITVTEARADGSTPESSGVPIAGPSDVRVVEAGNLVISIQVLRNVTNNFSPS 600

Query: 601 NILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG 660
           NILG GGFGTVYKGELHDGTKIAVKRMESGV+GEKGLNEFKAEIAVLTKVRHRNLVALLG
Sbjct: 601 NILGRGGFGTVYKGELHDGTKIAVKRMESGVVGEKGLNEFKAEIAVLTKVRHRNLVALLG 660

Query: 661 YCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQ 720
           YCLDGNERLLVYEYMPQGT SRFLF+W  EGI PLEWKRRL VALDVARGVEYLHSLAHQ
Sbjct: 661 YCLDGNERLLVYEYMPQGTFSRFLFHWNDEGIKPLEWKRRLTVALDVARGVEYLHSLAHQ 720

Query: 721 SFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780
           SFIHRDLKPSNILLGDD+RAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT
Sbjct: 721 SFIHRDLKPSNILLGDDLRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780

Query: 781 TKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQE 840
           TKVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRM+INKDTF KAIDPS+H+D+E
Sbjct: 781 TKVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMYINKDTFSKAIDPSMHIDEE 840

Query: 841 TLGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLP 900
           TLGSI TVADLAGHCCAREPYQRPDMSHAVNVLSSL+DVWKPTEPDSE+ LGIDLEMSLP
Sbjct: 841 TLGSICTVADLAGHCCAREPYQRPDMSHAVNVLSSLVDVWKPTEPDSEDTLGIDLEMSLP 900

Query: 901 QALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           QALK+WQAFEGNSN+D +SSSS+ LASGDNTQ+SIPNRP GFANSFTS+DAR
Sbjct: 901 QALKRWQAFEGNSNLDMTSSSSTLLASGDNTQSSIPNRPPGFANSFTSLDAR 951

BLAST of MC04g_new0454 vs. ExPASy TrEMBL
Match: A0A6J1K3X7 (receptor protein kinase TMK1-like OS=Cucurbita maxima OX=3661 GN=LOC111489829 PE=3 SV=1)

HSP 1 Score: 1575 bits (4077), Expect = 0.0
Identity = 784/952 (82.35%), Postives = 862/952 (90.55%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGF--YVVWAQPSGGDGDG-SVMVILRTSIGAPASLGWSGSD 60
           MREPH G GVFL V   FL F  + V+AQP+G  GD  +VM +L+ SIGAP SLGW+GS+
Sbjct: 1   MREPHLGFGVFLLVFVLFLLFLDFSVYAQPAGAGGDDETVMNLLKNSIGAPDSLGWTGSN 60

Query: 61  YCQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLAESL 120
           YC+WKHVKCDS+NRVVKIQIGNQNLKGSLPS+LFSLSALVQLEVQ NQL+GPFPNLA+SL
Sbjct: 61  YCEWKHVKCDSQNRVVKIQIGNQNLKGSLPSDLFSLSALVQLEVQSNQLTGPFPNLADSL 120

Query: 121 QILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNIT 180
           QILLAHDNLFTS+PADFFAKKS LQ I++DNNPF AWKIPD+IRDASGLQE+SANR N+T
Sbjct: 121 QILLAHDNLFTSMPADFFAKKSALQTIDVDNNPFMAWKIPDSIRDASGLQELSANRANVT 180

Query: 181 GTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQ 240
           G     FDGTTFPTLRNLHL+GNFLEGGLP SF+GSSI+SLWLNGQQSN KLNGS+AILQ
Sbjct: 181 GNTLQIFDGTTFPTLRNLHLAGNFLEGGLPVSFAGSSIRSLWLNGQQSNSKLNGSIAILQ 240

Query: 241 NMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNN 300
           NMTNLQE+WLH N FSGPLPDFSNL+GL+ L+LRDNQLTGIVP SL+N+KSLV+VNLTNN
Sbjct: 241 NMTNLQEVWLHMNHFSGPLPDFSNLRGLETLNLRDNQLTGIVPSSLINMKSLVVVNLTNN 300

Query: 301 MLQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGN 360
           MLQGP P F+ NV+LDMRPG+NNFC DSPG PCDPRV+ALLSVAQSM FPTVFA++W GN
Sbjct: 301 MLQGPTPAFNPNVKLDMRPGSNNFCTDSPGEPCDPRVSALLSVAQSMGFPTVFAQSWVGN 360

Query: 361 DPC-NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTE 420
            PC +FKGV C GN P N+T++NF+N+GLAG+ISPSFSLLTSV KL+LS+N LSG IPTE
Sbjct: 361 SPCEDFKGVACTGN-PENITIINFKNMGLAGSISPSFSLLTSVQKLILSNNYLSGTIPTE 420

Query: 421 LVTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPG 480
           LVTMPSL+ELDVS+NRLHGKVP+F KNVIVNT GNPDIGKDR S+P PGSP E +PSD G
Sbjct: 421 LVTMPSLIELDVSSNRLHGKVPAFGKNVIVNTQGNPDIGKDRPSSPAPGSPEEGAPSDYG 480

Query: 481 SGGLPVDVDKKSNSGVVVGSIIGVI-GLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPS 540
           +G  P    KKSN+GVVVGSIIGVI GL+LVG  IFF Y+R KR  S VQ+PNTVVVHPS
Sbjct: 481 TGDFPDGGVKKSNTGVVVGSIIGVIVGLLLVGTAIFFWYKRKKRHDSIVQNPNTVVVHPS 540

Query: 541 HSGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFSPE 600
           HSGDQN+VKIT+ E+R DG+ PE+ RVPI GPSDV VVEAGN+VISIQVLRNVTNNFSP 
Sbjct: 541 HSGDQNSVKITVTETRTDGSTPESSRVPIAGPSDVRVVEAGNLVISIQVLRNVTNNFSPS 600

Query: 601 NILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLG 660
           NILG GGFGTVYKGELHDGTKIAVKRMESGV+GEKGLNEFKAEIAVLTKVRHRNLVALLG
Sbjct: 601 NILGRGGFGTVYKGELHDGTKIAVKRMESGVVGEKGLNEFKAEIAVLTKVRHRNLVALLG 660

Query: 661 YCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQ 720
           YCLDGNERLLVYEYMPQGT SRFLF+W  EGI PLEWKRRL VALDVARGVEYLHSLAHQ
Sbjct: 661 YCLDGNERLLVYEYMPQGTFSRFLFHWNDEGIKPLEWKRRLTVALDVARGVEYLHSLAHQ 720

Query: 721 SFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780
           SFIHRDLKPSNILLGDD+RAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT
Sbjct: 721 SFIHRDLKPSNILLGDDLRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVT 780

Query: 781 TKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKDTFPKAIDPSIHLDQE 840
           TKVDVYSFGVILMEMI+GRKA+D+SQPEE LHLVSWFRRM+INKDTF KAIDPS+H+D+E
Sbjct: 781 TKVDVYSFGVILMEMISGRKAIDESQPEESLHLVSWFRRMYINKDTFSKAIDPSMHIDEE 840

Query: 841 TLGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLP 900
           TLGSI TVADLAGHCCAREPYQRPDMSHAVNVLSSL+DVWKPTEPDSE+ LGIDLEMSLP
Sbjct: 841 TLGSICTVADLAGHCCAREPYQRPDMSHAVNVLSSLVDVWKPTEPDSEDTLGIDLEMSLP 900

Query: 901 QALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 947
           QALK+WQAFEGNSN+D +SSSSS LASGDNTQ+SIPNRP GFANSFTS+DAR
Sbjct: 901 QALKRWQAFEGNSNLDMTSSSSSLLASGDNTQSSIPNRPPGFANSFTSLDAR 951

BLAST of MC04g_new0454 vs. TAIR 10
Match: AT1G66150.1 (transmembrane kinase 1 )

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 576/951 (60.57%), Postives = 725/951 (76.24%), Query Frame = 0

Query: 13  FVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQWKHVKCDSENRV 72
           F+L SF    ++    +  DGD S M+ L+ S+  P+S GWS  D C+W H+ C    RV
Sbjct: 7   FLLFSFTFLLLLSLSKADSDGDLSAMLSLKKSLNPPSSFGWSDPDPCKWTHIVCTGTKRV 66

Query: 73  VKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLA--ESLQILLAHDNLFTSL 132
            +IQIG+  L+G+L  +L +LS L +LE+Q N +SGP P+L+   SLQ+L+  +N F S+
Sbjct: 67  TRIQIGHSGLQGTLSPDLRNLSELERLELQWNNISGPVPSLSGLASLQVLMLSNNNFDSI 126

Query: 133 PADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTIPDFFDGTTFP 192
           P+D F   ++LQ +EIDNNPF +W+IP+++R+AS LQ  SAN  N++G++P F     FP
Sbjct: 127 PSDVFQGLTSLQSVEIDNNPFKSWEIPESLRNASALQNFSANSANVSGSLPGFLGPDEFP 186

Query: 193 TLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQNMTNLQEIWLHTN 252
            L  LHL+ N LEG LP S +GS +QSLWLNGQ    KL G + +LQNMT L+E+WLH+N
Sbjct: 187 GLSILHLAFNNLEGELPMSLAGSQVQSLWLNGQ----KLTGDITVLQNMTGLKEVWLHSN 246

Query: 253 QFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQGPAPVFDRNV 312
           +FSGPLPDFS L+ L++LSLRDN  TG VP SL++L+SL +VNLTNN LQGP PVF  +V
Sbjct: 247 KFSGPLPDFSGLKELESLSLRDNSFTGPVPASLLSLESLKVVNLTNNHLQGPVPVFKSSV 306

Query: 313 QLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGNDPC-NFKGVVCIG 372
            +D+   +N+FC+ SPG  CDPRV +LL +A S ++P   A++WKGNDPC N+ G+ C  
Sbjct: 307 SVDLDKDSNSFCLSSPG-ECDPRVKSLLLIASSFDYPPRLAESWKGNDPCTNWIGIAC-- 366

Query: 373 NSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTELVTMPSLVELDVS 432
            S  N+TV++   + L GTISP F  + S+ +++L  N L+G+IP EL T+P+L  LDVS
Sbjct: 367 -SNGNITVISLEKMELTGTISPEFGAIKSLQRIILGINNLTGMIPQELTTLPNLKTLDVS 426

Query: 433 NNRLHGKVPSFRKNVIVNTDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGLPVDVDK--- 492
           +N+L GKVP FR NV+VNT+GNPDIGKD+ S   PGS    SPS     G+  D D+   
Sbjct: 427 SNKLFGKVPGFRSNVVVNTNGNPDIGKDKSSLSSPGS---SSPSGGSGSGINGDKDRRGM 486

Query: 493 KSNS--GVVVGSII-GVIGLVLVGAVIFFLYRRNKRRSSRVQSPNTVVVHPSHSG-DQNA 552
           KS++  G++VGS++ G++ + L+G ++F  Y++ ++R S  +S N VVVHP HSG D  +
Sbjct: 487 KSSTFIGIIVGSVLGGLLSIFLIGLLVFCWYKKRQKRFSGSESSNAVVVHPRHSGSDNES 546

Query: 553 VKITIAESRAD-GNAPETGRVPIGGPSDV----HVVEAGNMVISIQVLRNVTNNFSPENI 612
           VKIT+A S    G   +T  +P  G S+V     +VEAGNM+ISIQVLR+VTNNFS +NI
Sbjct: 547 VKITVAGSSVSVGGISDTYTLP--GTSEVGDNIQMVEAGNMLISIQVLRSVTNNFSSDNI 606

Query: 613 LGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYC 672
           LGSGGFG VYKGELHDGTKIAVKRME+GVI  KG  EFK+EIAVLTKVRHR+LV LLGYC
Sbjct: 607 LGSGGFGVVYKGELHDGTKIAVKRMENGVIAGKGFAEFKSEIAVLTKVRHRHLVTLLGYC 666

Query: 673 LDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLAHQSF 732
           LDGNE+LLVYEYMPQGTLSR LF W +EG+ PL WK+RL +ALDVARGVEYLH LAHQSF
Sbjct: 667 LDGNEKLLVYEYMPQGTLSRHLFEWSEEGLKPLLWKQRLTLALDVARGVEYLHGLAHQSF 726

Query: 733 IHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTK 792
           IHRDLKPSNILLGDDMRAKVADFGLVRLAPEGK S+ETR+AGTFGYLAPEYAVTGRVTTK
Sbjct: 727 IHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKGSIETRIAGTFGYLAPEYAVTGRVTTK 786

Query: 793 VDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKD-TFPKAIDPSIHLDQET 852
           VDVYSFGVILME+ITGRK+LD+SQPEE +HLVSWF+RM+INK+ +F KAID +I LD+ET
Sbjct: 787 VDVYSFGVILMELITGRKSLDESQPEESIHLVSWFKRMYINKEASFKKAIDTTIDLDEET 846

Query: 853 LGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQ 912
           L S+ TVA+LAGHCCAREPYQRPDM HAVN+LSSL+++WKP++ + E++ GIDL+MSLPQ
Sbjct: 847 LASVHTVAELAGHCCAREPYQRPDMGHAVNILSSLVELWKPSDQNPEDIYGIDLDMSLPQ 906

Query: 913 ALKKWQAFEGNSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 948
           ALKKWQA+EG S  D  SS+SS L S DNTQ SIP RP GFA SFTSVD R
Sbjct: 907 ALKKWQAYEGRS--DLESSTSSLLPSLDNTQMSIPTRPYGFAESFTSVDGR 942

BLAST of MC04g_new0454 vs. TAIR 10
Match: AT2G01820.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 1047.0 bits (2706), Expect = 9.4e-306
Identity = 550/957 (57.47%), Postives = 697/957 (72.83%), Query Frame = 0

Query: 1   MREPHFGRGVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYCQ 60
           M   H G   F+  L     F +     S    D S M  L++S+   + + WS  + C+
Sbjct: 1   MSNSHLGTLCFIISLLGLANFSL-----SQTGLDDSTMQSLKSSLNLTSDVDWSNPNPCK 60

Query: 61  WKHVKCDSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGNQLSGPFPNLA--ESLQ 120
           W+ V+CD  NRV KIQ+  + ++G+LP+ L SLS LV LE+  N++SGP P+L+    LQ
Sbjct: 61  WQSVQCDGSNRVTKIQLKQKGIRGTLPTNLQSLSELVILELFLNRISGPIPDLSGLSRLQ 120

Query: 121 ILLAHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITG 180
            L  HDNLFTS+P + F+  S+LQ + ++NNPF  W IPD +++A+ LQ ++ +  +I G
Sbjct: 121 TLNLHDNLFTSVPKNLFSGMSSLQEMYLENNPFDPWVIPDTVKEATSLQNLTLSNCSIIG 180

Query: 181 TIPDFFDGTTFPTLRNLHLSGNFLEGGLPASFSGSSIQSLWLNGQQSNFKLNGSVAILQN 240
            IPDFF   + P+L NL LS N LEG LP SF+G+SIQSL+LNGQ    KLNGS+++L N
Sbjct: 181 KIPDFFGSQSLPSLTNLKLSQNGLEGELPMSFAGTSIQSLFLNGQ----KLNGSISVLGN 240

Query: 241 MTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRDNQLTGIVPPSLVNLKSLVIVNLTNNM 300
           MT+L E+ L  NQFSGP+PD S L  L+  ++R+NQLTG+VP SLV+L SL  VNLTNN 
Sbjct: 241 MTSLVEVSLQGNQFSGPIPDLSGLVSLRVFNVRENQLTGVVPQSLVSLSSLTTVNLTNNY 300

Query: 301 LQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPCDPRVNALLSVAQSMNFPTVFAKNWKGND 360
           LQGP P+F ++V +D+    N+FC +  G  CDPRV+ L+SVA+S  +P   A++WKGN+
Sbjct: 301 LQGPTPLFGKSVGVDIVNNMNSFCTNVAGEACDPRVDTLVSVAESFGYPVKLAESWKGNN 360

Query: 361 PC-NFKGVVCIGNSPVNVTVLNFRNLGLAGTISPSFSLLTSVTKLLLSDNLLSGVIPTEL 420
           PC N+ G+ C G    N+TV+N R   L+GTISPS + LTS+  + L+DN LSG IP EL
Sbjct: 361 PCVNWVGITCSGG---NITVVNMRKQDLSGTISPSLAKLTSLETINLADNKLSGHIPDEL 420

Query: 421 VTMPSLVELDVSNNRLHGKVPSFRKNVIVNTDGNPDIGKD--RRSTPPPGSPTEKSPSDP 480
            T+  L  LDVSNN  +G  P FR  V + T+GN ++GK+   +++  PG+     PS  
Sbjct: 421 TTLSKLRLLDVSNNDFYGIPPKFRDTVTLVTEGNANMGKNGPNKTSDAPGASPGSKPSG- 480

Query: 481 GSGGLPVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQSPNT-VVVHP 540
           GS G      K SN  ++V  + GV+G + +  +   LY + ++R +RVQSP++ +V+HP
Sbjct: 481 GSDGSETS-KKSSNVKIIVPVVGGVVGALCLVGLGVCLYAKKRKRPARVQSPSSNMVIHP 540

Query: 541 SHSGDQNAVKITIAESRAD-GNAPETGRVPIGGPSDVHVVEAGNMVISIQVLRNVTNNFS 600
            HSGD + +K+T+A S  + G   ++        SD+HVVEAGN+VISIQVLRNVTNNFS
Sbjct: 541 HHSGDNDDIKLTVAASSLNSGGGSDSYSHSGSAASDIHVVEAGNLVISIQVLRNVTNNFS 600

Query: 601 PENILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKVRHRNLVAL 660
            ENILG GGFGTVYKGELHDGTKIAVKRMES V+ +KGL EFK+EI VLTK+RHR+LVAL
Sbjct: 601 EENILGRGGFGTVYKGELHDGTKIAVKRMESSVVSDKGLTEFKSEITVLTKMRHRHLVAL 660

Query: 661 LGYCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARGVEYLHSLA 720
           LGYCLDGNERLLVYEYMPQGTLS+ LF+WK+EG  PL+W RRL +ALDVARGVEYLH+LA
Sbjct: 661 LGYCLDGNERLLVYEYMPQGTLSQHLFHWKEEGRKPLDWTRRLAIALDVARGVEYLHTLA 720

Query: 721 HQSFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKASVETRLAGTFGYLAPEYAVTGR 780
           HQSFIHRDLKPSNILLGDDMRAKV+DFGLVRLAP+GK S+ETR+AGTFGYLAPEYAVTGR
Sbjct: 721 HQSFIHRDLKPSNILLGDDMRAKVSDFGLVRLAPDGKYSIETRVAGTFGYLAPEYAVTGR 780

Query: 781 VTTKVDVYSFGVILMEMITGRKALDDSQPEECLHLVSWFRRMHINKD--TFPKAIDPSIH 840
           VTTKVD++S GVILME+ITGRKALD++QPE+ +HLV+WFRR+  +KD   F  AIDP+I 
Sbjct: 781 VTTKVDIFSLGVILMELITGRKALDETQPEDSVHLVTWFRRVAASKDENAFKNAIDPNIS 840

Query: 841 LDQETLGSISTVADLAGHCCAREPYQRPDMSHAVNVLSSLIDVWKPTEPDSEEVLGIDLE 900
           LD +T+ SI  V +LAGHCCAREPYQRPDM+H VNVLSSL   WKPTE D ++V GID +
Sbjct: 841 LDDDTVASIEKVWELAGHCCAREPYQRPDMAHIVNVLSSLTVQWKPTETDPDDVYGIDYD 900

Query: 901 MSLPQALKKWQAFEG-NSNMDASSSSSSFLASGDNTQTSIPNRPSGFANSFTSVDAR 948
           M LPQ LKKWQAFEG +   D S SSSS   S DNTQTSIP RPSGFA+SFTSVD R
Sbjct: 901 MPLPQVLKKWQAFEGLSQTADDSGSSSSAYGSKDNTQTSIPTRPSGFADSFTSVDGR 943

BLAST of MC04g_new0454 vs. TAIR 10
Match: AT1G24650.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 913.3 bits (2359), Expect = 1.6e-265
Identity = 500/924 (54.11%), Postives = 640/924 (69.26%), Query Frame = 0

Query: 34  DGSVMVILRTSIGAPASLGWSGSDYCQWK-HVKCDSENRVVKIQIGNQNLKGSLPSELFS 93
           D +VM+ LR S+    +  WSGSD C+W   +KCD+ NRV  IQIG++ + G LP +L  
Sbjct: 23  DEAVMIALRDSLKLSGNPNWSGSDPCKWSMFIKCDASNRVTAIQIGDRGISGKLPPDLGK 82

Query: 94  LSALVQLEVQGNQLSGPFPNLA--ESLQILLAHDNLFTSLPADFFAKKSTLQMIEIDNNP 153
           L++L + EV  N+L+GP P+LA  +SL  + A+DN FTS+P DFF+  S+LQ + +DNNP
Sbjct: 83  LTSLTKFEVMRNRLTGPIPSLAGLKSLVTVYANDNDFTSVPEDFFSGLSSLQHVSLDNNP 142

Query: 154 FSAWKIPDNIRDASGLQEISANRVNITGTIPDF-FDGTTFPTLRNLHLSGNFLEGGLPAS 213
           F +W IP ++ +A+ L + SA   N++G IPD+ F+G  F +L  L LS N L    P +
Sbjct: 143 FDSWVIPPSLENATSLVDFSAVNCNLSGKIPDYLFEGKDFSSLTTLKLSYNSLVCEFPMN 202

Query: 214 FSGSSIQSLWLNGQQSNFKLNGSVAILQNMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLS 273
           FS S +Q L LNGQ+   KL+GS++ LQ MT+L  + L  N FSGPLPDFS L  L++ +
Sbjct: 203 FSDSRVQVLMLNGQKGREKLHGSISFLQKMTSLTNVTLQGNSFSGPLPDFSGLVSLKSFN 262

Query: 274 LRDNQLTGIVPPSLVNLKSLVIVNLTNNMLQGPAPVFDR-NVQLDMRPGTNNFCVDSPGV 333
           +R+NQL+G+VP SL  L+SL  V L NN+LQGP P F   +++ D+  G N+FC+D+PG 
Sbjct: 263 VRENQLSGLVPSSLFELQSLSDVALGNNLLQGPTPNFTAPDIKPDLN-GLNSFCLDTPGT 322

Query: 334 PCDPRVNALLSVAQSMNFPTVFAKNWKGNDPCN-FKGVVCIGNSPVNVTVLNFRNLGLAG 393
            CDPRVN LLS+ ++  +P  FA+ WKGNDPC+ + G+ C G    ++TV+NF+NLGL G
Sbjct: 323 SCDPRVNTLLSIVEAFGYPVNFAEKWKGNDPCSGWVGITCTG---TDITVINFKNLGLNG 382

Query: 394 TISPSFSLLTSVTKLLLSDNLLSGVIPTELVTMPSLVELDVSNNRLHGKVPSFRKNVIVN 453
           TISP F+   S+  + LS N L+G IP EL  + +L  LDVS NRL G+VP F    IVN
Sbjct: 383 TISPRFADFASLRVINLSQNNLNGTIPQELAKLSNLKTLDVSKNRLCGEVPRF-NTTIVN 442

Query: 454 TDGNPDIGKDRRSTPPPGSPTEKSPSDPGSGGLPVDVDKKSNSGVVVGSIIGV-IGLVLV 513
           T GN +         P G+  +K+                SN+G +VGS+IG+ + L+L+
Sbjct: 443 TTGNFE-------DCPNGNAGKKA---------------SSNAGKIVGSVIGILLALLLI 502

Query: 514 GAVIFFLYRRNKRRSSRVQSPNTVVVHP-SHSGDQNAVKITIAESRADGNAPETGRVPIG 573
           G  IFFL ++  +            +HP   S DQ+A KITI E+   G   E+G     
Sbjct: 503 GVAIFFLVKKKMQYHK---------MHPQQQSSDQDAFKITI-ENLCTG-VSESG----F 562

Query: 574 GPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGSGGFGTVYKGELHDGTKIAVKRMESG 633
             +D H+ EAGN+VISIQVLR+ T NF  +NILG GGFG VYKGELHDGTKIAVKRMES 
Sbjct: 563 SGNDAHLGEAGNIVISIQVLRDATYNFDEKNILGRGGFGIVYKGELHDGTKIAVKRMESS 622

Query: 634 VIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDGNERLLVYEYMPQGTLSRFLFYWKKE 693
           +I  KGL+EFK+EIAVLT+VRHRNLV L GYCL+GNERLLVY+YMPQGTLSR +FYWK+E
Sbjct: 623 IISGKGLDEFKSEIAVLTRVRHRNLVVLHGYCLEGNERLLVYQYMPQGTLSRHIFYWKEE 682

Query: 694 GINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHRDLKPSNILLGDDMRAKVADFGLVRL 753
           G+ PLEW RRLI+ALDVARGVEYLH+LAHQSFIHRDLKPSNILLGDDM AKVADFGLVRL
Sbjct: 683 GLRPLEWTRRLIIALDVARGVEYLHTLAHQSFIHRDLKPSNILLGDDMHAKVADFGLVRL 742

Query: 754 APEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMEMITGRKALDDSQPEEC 813
           APEG  S+ET++AGTFGYLAPEYAVTGRVTTKVDVYSFGVILME++TGRKALD ++ EE 
Sbjct: 743 APEGTQSIETKIAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMELLTGRKALDVARSEEE 802

Query: 814 LHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSISTVADLAGHCCAREPYQRPDMSHAV 873
           +HL +WFRRM INK +FPKAID ++ +++ETL SI+ VA+LA  C +REP  RPDM+H V
Sbjct: 803 VHLATWFRRMFINKGSFPKAIDEAMEVNEETLRSINIVAELANQCSSREPRDRPDMNHVV 862

Query: 874 NVLSSLIDVWKPTE--PDSEEVLGIDLEMSLPQALKKWQAFEGNSNMDASSSSSSFLASG 933
           NVL SL+  WKPTE   DSE++ GID +  LPQ +                  S F   G
Sbjct: 863 NVLVSLVVQWKPTERSSDSEDIYGIDYDTPLPQLI----------------LDSCFF--G 886

Query: 934 DNTQTSIPNRPSGFANSFTSVDAR 948
           DNT TSIP+RPS   ++F S   R
Sbjct: 923 DNTLTSIPSRPSELESTFKSGQGR 886

BLAST of MC04g_new0454 vs. TAIR 10
Match: AT3G23750.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 808.9 bits (2088), Expect = 4.3e-234
Identity = 451/926 (48.70%), Postives = 598/926 (64.58%), Query Frame = 0

Query: 34  DGSVMVILRTSIGAPASLGWSGSDYCQWKHVKCDSENRVVKIQIGNQNLKGSLPSELFSL 93
           D + M+ L  S   P S   S +D+C+W  V+C +  RV  I + +++L G +  E+ +L
Sbjct: 26  DQTAMLALAKSFNPPPSDWSSTTDFCKWSGVRC-TGGRVTTISLADKSLTGFIAPEISTL 85

Query: 94  SALVQLEVQGNQLSGPFPNLAE--SLQILLAHDNLFTSLPADFFAKKSTLQMIEI-DNNP 153
           S L  + +Q N+LSG  P+ A+  SLQ +   +N F  +    FA  ++LQ++ + DNN 
Sbjct: 86  SELKSVSIQRNKLSGTIPSFAKLSSLQEIYMDENNFVGVETGAFAGLTSLQILSLSDNNN 145

Query: 154 FSAWKIPDNIRDASGLQEISANRVNITGTIPDFFDGTTFPTLRNLHLSGNFLEGGLPASF 213
            + W  P  + D++ L  I  +  NI G +PD FD  +  +L+NL LS N + G LP S 
Sbjct: 146 ITTWSFPSELVDSTSLTTIYLDNTNIAGVLPDIFD--SLASLQNLRLSYNNITGVLPPSL 205

Query: 214 SGSSIQSLWLNGQQSNFKLNGSVAILQNMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSL 273
             SSIQ+LW+N Q  +  ++G++ +L +MT+L + WLH N F GP+PD S  + L +L L
Sbjct: 206 GKSSIQNLWINNQ--DLGMSGTIEVLSSMTSLSQAWLHKNHFFGPIPDLSKSENLFDLQL 265

Query: 274 RDNQLTGIVPPSLVNLKSLVIVNLTNNMLQGPAPVFDRNVQLDMRPGTNNFCVDSPGVPC 333
           RDN LTGIVPP+L+ L SL  ++L NN  QGP P+F   V++ +    N FC    G  C
Sbjct: 266 RDNDLTGIVPPTLLTLASLKNISLDNNKFQGPLPLFSPEVKVTI--DHNVFCTTKAGQSC 325

Query: 334 DPRVNALLSVAQSMNFPTVFAKNWKGNDPCNFKGVVCIGNSPVNVTVLNFRNLGLAGTIS 393
            P+V  LL+VA  + +P++ A++W+G+D C+    V   ++  NV  LN    G  G IS
Sbjct: 326 SPQVMTLLAVAGGLGYPSMLAESWQGDDACSGWAYVSCDSAGKNVVTLNLGKHGFTGFIS 385

Query: 394 PSFSLLTSVTKLLLSDNLLSGVIPTELVTMPSLVELDVSNNRLHGKVPSFRKNVIVN-TD 453
           P+ + LTS+  L L+ N L+GVIP EL  M SL  +DVSNN L G++P F   V  +   
Sbjct: 386 PAIANLTSLKSLYLNGNDLTGVIPKELTFMTSLQLIDVSNNNLRGEIPKFPATVKFSYKP 445

Query: 454 GNPDIGKDRRSTPPPGSPTEKSPSDPGSGGLPVDVDKKSNSGVVVGSIIGV-IGLVLVGA 513
           GN  +G +      PG+          SGG        S  GV+VG I+ V + L ++G 
Sbjct: 446 GNALLGTNGGDGSSPGTGGASGGPGGSSGG------GGSKVGVIVGVIVAVLVFLAILGF 505

Query: 514 VIF-FLYRRNKRRSSRV--QSPNTVVVHP--SHSGDQNAVKITIAESRADGNAPETGRVP 573
           V++ F+ +R   R +R   +    ++V    S+ G  N      A      N        
Sbjct: 506 VVYKFVMKRKYGRFNRTDPEKVGKILVSDAVSNGGSGNG---GYANGHGANNFNALNSPS 565

Query: 574 IGGPSDVHVVEAGNMVISIQVLRNVTNNFSPENILGSGGFGTVYKGELHDGTKIAVKRME 633
            G  SD  ++E G++ I ++VLR VTNNFS +NILG GGFG VY GELHDGTK AVKRME
Sbjct: 566 SGDNSDRFLLEGGSVTIPMEVLRQVTNNFSEDNILGRGGFGVVYAGELHDGTKTAVKRME 625

Query: 634 SGVIGEKGLNEFKAEIAVLTKVRHRNLVALLGYCLDGNERLLVYEYMPQGTLSRFLFYWK 693
              +G KG++EF+AEIAVLTKVRHR+LVALLGYC++GNERLLVYEYMPQG L + LF W 
Sbjct: 626 CAAMGNKGMSEFQAEIAVLTKVRHRHLVALLGYCVNGNERLLVYEYMPQGNLGQHLFEWS 685

Query: 694 KEGINPLEWKRRLIVALDVARGVEYLHSLAHQSFIHRDLKPSNILLGDDMRAKVADFGLV 753
           + G +PL WK+R+ +ALDVARGVEYLHSLA QSFIHRDLKPSNILLGDDMRAKVADFGLV
Sbjct: 686 ELGYSPLTWKQRVSIALDVARGVEYLHSLAQQSFIHRDLKPSNILLGDDMRAKVADFGLV 745

Query: 754 RLAPEGKASVETRLAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMEMITGRKALDDSQPE 813
           + AP+GK SVETRLAGTFGYLAPEYA TGRVTTKVDVY+FGV+LME++TGRKALDDS P+
Sbjct: 746 KNAPDGKYSVETRLAGTFGYLAPEYAATGRVTTKVDVYAFGVVLMEILTGRKALDDSLPD 805

Query: 814 ECLHLVSWFRRMHINKDTFPKAIDPSIHLDQETLGSISTVADLAGHCCAREPYQRPDMSH 873
           E  HLV+WFRR+ INK+  PKA+D ++  D+ET+ SI  VA+LAGHC AREP QRPDM H
Sbjct: 806 ERSHLVTWFRRILINKENIPKALDQTLEADEETMESIYRVAELAGHCTAREPQQRPDMGH 865

Query: 874 AVNVLSSLIDVWKPTEPDSEEVLGIDLEMSLPQALKKWQAFEGNSNMDASSSSSSFLA-- 933
           AVNVL  L++ WKP+  + EE  GID+ MSLPQAL++WQ        + +SSS+ F    
Sbjct: 866 AVNVLGPLVEKWKPSCQEEEESFGIDVNMSLPQALQRWQ-------NEGTSSSTMFHGDF 925

Query: 934 SGDNTQTSIPNRPSGFANSFTSVDAR 948
           S   TQ+SIP + SGF N+F S D R
Sbjct: 926 SYSQTQSSIPPKASGFPNTFDSADGR 928

BLAST of MC04g_new0454 vs. TAIR 10
Match: AT5G49770.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 304.7 bits (779), Expect = 2.6e-82
Identity = 290/951 (30.49%), Postives = 428/951 (45.01%), Query Frame = 0

Query: 9   GVFLFVLTSFLGFYVVWAQPSGGDGDGSVMVILRTSIGAPASLGWSGSDYC--QWKHVKC 68
           G+F  ++  F   Y V+A   G   D + +  L+       S  W  SD C  +W  + C
Sbjct: 8   GLFKLLILLFFQIYSVYAFTDG--SDFTALQALKNEWDT-LSKSWKSSDPCGTEWVGITC 67

Query: 69  DSENRVVKIQIGNQNLKGSLPSELFSLSALVQLEVQGN-QLSGPFP----NLAESLQILL 128
           +++NRVV I + N+NLKG LP+E+ +LS L  L++ GN +LSGP P    NL   L  L 
Sbjct: 68  NNDNRVVSISLTNRNLKGKLPTEISTLSELQTLDLTGNPELSGPLPANIGNL-RKLTFLS 127

Query: 129 AHDNLFTSLPADFFAKKSTLQMIEIDNNPFSAWKIPDNIRDASGLQEISANRVNITGTIP 188
                F     D       L  + ++ N FS   IP ++   S L         + G +P
Sbjct: 128 LMGCAFNGPIPDSIGNLEQLTRLSLNLNKFSG-TIPASMGRLSKLYWFDIADNQLEGKLP 187

Query: 189 DFFDGTTFPTLRNL------HLSGNFLEGGLPA-----------------SFSGSSIQSL 248
              DG + P L  L      H   N L G +P                   F+GS  +SL
Sbjct: 188 -VSDGASLPGLDMLLQTGHFHFGNNKLSGEIPEKLFSSEMTLLHVLFDGNQFTGSIPESL 247

Query: 249 WLNG-----QQSNFKLNGSV-AILQNMTNLQEIWLHTNQFSGPLPDFSNLQGLQNLSLRD 308
            L       +    +L+G + + L N+TNLQE+ L  N+F+G LP+ ++L  L  L + +
Sbjct: 248 GLVQNLTVLRLDRNRLSGDIPSSLNNLTNLQELHLSDNKFTGSLPNLTSLTSLYTLDVSN 307

Query: 309 NQLT-GIVPPSLVNLKSLVIVNLTNNMLQGPAP--VFDR-------------NVQLDMRP 368
           N L    VP  +  L SL  + L +  L GP P  +F               N  LD+  
Sbjct: 308 NPLALSPVPSWIPFLNSLSTLRLEDIQLDGPVPTSLFSPLQLQTVSLKHNLINTTLDL-- 367

Query: 369 GTNNFCVDSPGVPCDPRVNALLSVAQSMNFP--TVFAKNWKGNDPCNFKGVVCIGNSPVN 428
           GT N+      V  D R N +       N P   + A N    DP N     C    P N
Sbjct: 368 GT-NYSKQLDFV--DLRDNFITGYKSPANNPVNVMLADNQVCQDPANQLSGYCNAVQP-N 427

Query: 429 VTVLNFRNLG------------------LAGTI---SPSFSLLTSVTKLL-LSDNLL--- 488
            T       G                  L G     SPSFS  ++ +  L   ++L+   
Sbjct: 428 STFSTLTKCGNHCGKGKEPNQGCHCVYPLTGVFTLRSPSFSGFSNNSNFLKFGESLMTFF 487

Query: 489 -SGVIPTELVTMPSLVELDVSNNRLHGKV--PSFRKNV-IVNTDGNPDIGKDRRSTPPPG 548
            +G  P + V M ++ E     + L   +  PS R        D        +   PPP 
Sbjct: 488 KNGKYPVDSVAMRNISENPTDYHLLINLLIFPSGRDRFNQTEMDSINSAFTIQDYKPPPR 547

Query: 549 SPTEKSPSDPGSGGLPVDVDKKSNSGVVVGSIIGVIGLVLVGAVIFFLYRRNKRRSSRVQ 608
                  +D       ++  K  +  V++G ++GV+ L+L+ A+      R K+R+ R  
Sbjct: 548 FGPYIFVADQYKTFSDLEDSKTVSMKVIIGVVVGVVVLLLLLALAGIYALRQKKRAQRA- 607

Query: 609 SPNTVVVHPSHSGDQNAVKITIAESRADGNAPETGRVPIGGPSDVHVVEAGNMVISIQVL 668
              T  ++P    D           + + +AP+                 G    + + L
Sbjct: 608 ---TDQMNPFAKWD---------AGKNEMDAPQL---------------MGTKAFTFEEL 667

Query: 669 RNVTNNFSPENILGSGGFGTVYKGELHDGTKIAVKRMESGVIGEKGLNEFKAEIAVLTKV 728
              TNNFS  N +G GG+G VYKG L +G  IA+KR + G +  +G  EFK EI +L++V
Sbjct: 668 SKCTNNFSDANDVGGGGYGQVYKGTLPNGQVIAIKRAQQGSM--QGAFEFKTEIELLSRV 727

Query: 729 RHRNLVALLGYCLDGNERLLVYEYMPQGTLSRFLFYWKKEGINPLEWKRRLIVALDVARG 788
            H+N+V LLG+C D  E++LVYEY+P G+L   L    K G+  L+W RRL +AL   +G
Sbjct: 728 HHKNVVKLLGFCFDQKEQMLVYEYIPNGSLRDGLS--GKNGVK-LDWTRRLKIALGSGKG 787

Query: 789 VEYLHSLAHQSFIHRDLKPSNILLGDDMRAKVADFGLVRLA--PEGKASVETRLAGTFGY 848
           + YLH LA    IHRD+K +NILL + + AKVADFGL +L   PE KA V T++ GT GY
Sbjct: 788 LAYLHELADPPIIHRDVKSNNILLDEHLTAKVADFGLSKLVGDPE-KAHVTTQVKGTMGY 847

Query: 849 LAPEYAVTGRVTTKVDVYSFGVILMEMITGRKALDDSQ--PEECLHLVSWFRRMHINKDT 873
           L PEY +T ++T K DVY FGV+++E++TG+  +D      +E    +   R ++     
Sbjct: 848 LDPEYYMTNQLTEKSDVYGFGVVMLELLTGKSPIDRGSYVVKEVKKKMDKSRNLY----D 907