MC04g_new0050 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g_new0050
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	O-methyltransferase family protein
Location	MC04: 1789648 .. 1791250 (+)
RNA-Seq Expression	MC04g_new0050
Synteny	MC04g_new0050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTGGAGCAGGGTGGAGAAACGGCAATAGATGAGATTCTTGAAGCTCAATCTCACATATGCAGCCACATCTTCAGCTTCATCAACCCCATGGCTCTCAAATGTGCAGTCCAATTAGGAATCCCAGACGCCATCCACCGCCATGGCCCCAACCCCATGCCCCTCTCTCAGCTCGTTTCAGCCCTCCAACTCAGCCCTCAAAAGACCCCTCACGTATACCGCTTGATGCGTCTTTTGGTTCACTTGGGTTTCTTTGTAATGCAAAAACTGAGCCAAAACGGCGAACAAGAGAAGGAAGAGGAGGGATATGTTCTGACCAATTCGTCTCGCTTTCTTCTCAAAGACAACCCTTTGACTCTGACTCCTTTCGTGCTCTCCATGCTCGACCCGGGCCTCGTCGAGCCATGGCGCTTCCTCTCGGCCTCGTTCCAAAGTAGTGATGATCATAGAACGGCATTTGATATGGCTCATGGAACACCCTTTTGGGAGTTCATGGCCAGTAGGCAAAAAGATAGTGATGTTTTTAATGCAGGCATGGCGAGTGATGCAAAGTTGGTGATTGGTGTTTTGTTGGAGAAATATAAAGGTGTTTTTGAAGGGCTTGGCTCGGTTGTTGATGTCGGAGGTGGCACTGGAGCTTTGGCCAAAGCCATTGCTGAGGCTTTTCCACAAATAGAATGCACTGTGTTGGATCTTCCCCAAGTTGTGGCTGACTTCAAAGGAAATGGGAAGAACAACTTGAATTATGTTGGAGGGGACATGTTTGAAGCCATTCCTCCTGCCAATGCACTTCTTTTGAAGGTATACTTGATTTTTTTCTCATACAAGCAAATTATTGCACGTCACTAAGAATTTTCGTTTTGCCGTTCTTGACGACGAAGAATTCTTCGTCTTCGTAGGGTACTTTTAGCGACATAATTGTTTGTCACAAAAAGTTTTGTCTGATTTGAGATTTTTTTAGACAAAATATTGGCGACGAAATTCATCGCAATAAGGTGCTTTTCGTGACTACTGTTGCATTTCTTCGTCAAAAGAATTTTGTTGTCAAAAATTATTGGAGGATAAGAGAACAAAACTTCTGGGATTTACTCGCAATAGGAGTACTTTTGAGGATGAAATTTAGTACGTGTATTTACTATATTTGTGTCAAAAACTTTTAGGATTTCGTCACAGAGTACTTTGACGACGAAATATAATACACGTATATATTATATTTATTTGTATATATTATATTTATTTGTATCGACAAAATGTAATATGTGTATATTATATGTGTGTGACAAATCAAACACAGTGGGTATTACATGATTGGAGCGATGAAGGATGCATAAAGATATTGAAGAAATGCAAAGAGGCAATCACAGAACATGGAAAGAAAGGGAAAGTGATGGTGATTGAAATGGTGGTAGGCAATAAGAAAATGGATGACAAGGTTGTTCAAACTCAACTATTCTTCGATATCTTGTTGATGACTATGTTGGTGGGTGGAAGAGAGCGAAGCGAGGAAGAGTGGGCTCAAATATTCTATGCCTCTGGATTTAGTGACTACAAAATATTTCCAATTCTGGGTTTAAGGTCTCTCATGGAGATTTACCCATAA

mRNA sequence

ATGGATTTGGAGCAGGGTGGAGAAACGGCAATAGATGAGATTCTTGAAGCTCAATCTCACATATGCAGCCACATCTTCAGCTTCATCAACCCCATGGCTCTCAAATGTGCAGTCCAATTAGGAATCCCAGACGCCATCCACCGCCATGGCCCCAACCCCATGCCCCTCTCTCAGCTCGTTTCAGCCCTCCAACTCAGCCCTCAAAAGACCCCTCACGTATACCGCTTGATGCGTCTTTTGTGGGTATTACATGATTGGAGCGATGAAGGATGCATAAAGATATTGAAGAAATGCAAAGAGGCAATCACAGAACATGGAAAGAAAGGGAAAGTGATGGTGATTGAAATGGTGGTAGGCAATAAGAAAATGGATGACAAGGTTGTTCAAACTCAACTATTCTTCGATATCTTGTTGATGACTATGTTGGTGGGTGGAAGAGAGCGAAGCGAGGAAGAGTGGGCTCAAATATTCTATGCCTCTGGATTTAGTGACTACAAAATATTTCCAATTCTGGGTTTAAGGTCTCTCATGGAGATTTACCCATAA

Coding sequence (CDS)

ATGGATTTGGAGCAGGGTGGAGAAACGGCAATAGATGAGATTCTTGAAGCTCAATCTCACATATGCAGCCACATCTTCAGCTTCATCAACCCCATGGCTCTCAAATGTGCAGTCCAATTAGGAATCCCAGACGCCATCCACCGCCATGGCCCCAACCCCATGCCCCTCTCTCAGCTCGTTTCAGCCCTCCAACTCAGCCCTCAAAAGACCCCTCACGTATACCGCTTGATGCGTCTTTTGTGGGTATTACATGATTGGAGCGATGAAGGATGCATAAAGATATTGAAGAAATGCAAAGAGGCAATCACAGAACATGGAAAGAAAGGGAAAGTGATGGTGATTGAAATGGTGGTAGGCAATAAGAAAATGGATGACAAGGTTGTTCAAACTCAACTATTCTTCGATATCTTGTTGATGACTATGTTGGTGGGTGGAAGAGAGCGAAGCGAGGAAGAGTGGGCTCAAATATTCTATGCCTCTGGATTTAGTGACTACAAAATATTTCCAATTCTGGGTTTAAGGTCTCTCATGGAGATTTACCCATAA

Protein sequence

MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLVSALQLSPQKTPHVYRLMRLLWVLHDWSDEGCIKILKKCKEAITEHGKKGKVMVIEMVVGNKKMDDKVVQTQLFFDILLMTMLVGGRERSEEEWAQIFYASGFSDYKIFPILGLRSLMEIYP

Homology

[Close]

BLAST of MC04g_new0050 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.3e-31
Identity = 107/355 (30.14%), Postives = 140/355 (39.44%), Query Frame = 0


Query: 13  EILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLVSALQLSPQKTPH 72
           EIL AQ+HI + IF+FIN M+LKCA+QLGIPD I+ HG  PM +SQL  AL ++ +K+P 
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPINRKKSPC 84

Query: 73  VYRLMRLL---------------------------------------------------- 132
           VYRLMR+L                                                    
Sbjct: 85  VYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDPV 144

Query: 133 ------------------------------------------------------------ 182
                                                                       
Sbjct: 145 MTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVIEK 204

[Close]

BLAST of MC04g_new0050 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 2.4e-29
Identity = 99/361 (27.42%), Postives = 134/361 (37.12%), Query Frame = 0


Query: 1   MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV 60
           MDL  G  +A  E+L AQ+H+ +HIF+FI  M+LKCA+QLGIPD IH HG  PM L +LV
Sbjct: 1   MDLANGVISA--ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPELV 60

Query: 61  SALQLSPQKTPHVYRLMRLL---------------------------------------- 120
           + L + P+++  VYRLMR+L                                        
Sbjct: 61  AKLPVHPKRSQCVYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIRPL 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 VLAMLDPILTKPWHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARL 180

Query: 181 ------------------------------------------------------------ 182
                                                                       
Sbjct: 181 LTSVLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSKNL 240

[Close]

BLAST of MC04g_new0050 vs. ExPASy Swiss-Prot
Match: A8QW53 (5-pentadecatrienyl resorcinol O-methyltransferase OS=Sorghum bicolor OX=4558 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.9e-28
Identity = 64/148 (43.24%), Postives = 94/148 (63.51%), Query Frame = 0


Query: 34  LKCAVQLGIPDAIHRHGPNPMPLSQLVSALQLSPQKTPHVYRLMRLLWVLHDWSDEGCIK 93
           LKC V L +P  + +   + +   Q V           +V   + L W+LHDWS++ CIK
Sbjct: 233 LKCTV-LDLPHVVAKAPSSSIGNVQFVGGDMFESIPPANV---VLLKWILHDWSNDECIK 292

Query: 94  ILKKCKEAITEHGKKGKVMVIEMVVGNKKMDDKVVQTQLFFDILLMTMLVGGRERSEEEW 153
           ILK CK+AI      GK+++I++VVG+   D K+++TQ+ +D+ LM   +GG ER E+EW
Sbjct: 293 ILKNCKQAIPSRDAGGKIIIIDVVVGSDSSDTKLLETQVIYDLHLMK--IGGVERDEQEW 352

Query: 154 AQIFYASGFSDYKIFPILGLRSLMEIYP 182
            +IF  +GF DYKI PILGLRS++E+YP
Sbjct: 353 KKIFLEAGFKDYKIMPILGLRSIIELYP 374

[Close]

BLAST of MC04g_new0050 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 7.2e-26
Identity = 89/363 (24.52%), Postives = 139/363 (38.29%), Query Frame = 0


Query: 7   GETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLVSALQLS 66
           G ++  E+LEAQ+H+ +HI+S+IN M+LKCA+QLGIPDAIH+HG NP+ LSQL  AL ++
Sbjct: 6   GISSKQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNIN 65

Query: 67  PQKTPHVYRLMRLL---------------------------------------------- 126
             K+  ++RLMR+L                                              
Sbjct: 66  KAKSHGLFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAP 125

Query: 127 ------------------------------------------------------------ 182
                                                                       
Sbjct: 126 FALAMSDPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAG 185

[Close]

BLAST of MC04g_new0050 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.4e-26
Identity = 94/367 (25.61%), Postives = 136/367 (37.06%), Query Frame = 0


Query: 1   MDLE----QGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPL 60
           MDLE     G   + +E+L AQ+HI +HIF+FI+ MALKCAV+L IP+ +H H P  + L
Sbjct: 7   MDLEIRNGNGYGDSGEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTL 66

Query: 61  SQLVSALQLSPQKTPHVYRLMRLL------------------------------------ 120
           S+LV ALQ+   K+  +YRL+R+L                                    
Sbjct: 67  SELVQALQIPQAKSACLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLKNHPMS 126

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 127 MSPCLLGLVNPTMVAPMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEIFNEAMG 186

Query: 181 ------------------------------------------------------------ 182
                                                                       
Sbjct: 187 CETRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLK 246

[Close]

BLAST of MC04g_new0050 vs. NCBI nr
Match: XP_022136005.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 293 bits (751), Expect = 2.69e-96
Identity = 181/369 (49.05%), Postives = 181/369 (49.05%), Query Frame = 0


Query: 1   MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV 60
           MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV
Sbjct: 1   MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV 60

Query: 61  SALQLSPQKTPHVYRLMRLL---------------------------------------- 120
           SALQLSPQKTPHVYRLMRLL                                        
Sbjct: 61  SALQLSPQKTPHVYRLMRLLVHLGFFVMQKLSQNGEQEKEEEGYVLTNSSRFLLKDNPLT 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 LTPFVLSMLDPGLVEPWRFLSASFQSSDDHRTAFDMAHGTPFWEFMASRQKDSDVFNAGM 180

[Close]

BLAST of MC04g_new0050 vs. NCBI nr
Match: XP_022135736.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 218 bits (556), Expect = 7.07e-67
Identity = 142/366 (38.80%), Postives = 163/366 (44.54%), Query Frame = 0


Query: 1   MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV 60
           MDLEQG E A+DEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIH HGPNPMPLSQLV
Sbjct: 1   MDLEQGEEMAVDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHSHGPNPMPLSQLV 60

Query: 61  SALQLSPQKTPHVYRLMRLL---------------------------------------- 120
           SALQLSPQKTPH+YRLMRLL                                        
Sbjct: 61  SALQLSPQKTPHIYRLMRLLVHSGFFVMHKLSSQNGAEQKEEEGYVLTNSSRLFLKDNPL 120

Query: 121 -----------------------------------------W------------------ 180
                                                    W                  
Sbjct: 121 NLAPYLLYMFEPFRVEPWQFLSAWFRSEDRTPFDTAHGMSFWEYMGRKPRDSEVFNAAMA 180

[Close]

BLAST of MC04g_new0050 vs. NCBI nr
Match: XP_022135671.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 200 bits (508), Expect = 1.08e-59
Identity = 132/364 (36.26%), Postives = 161/364 (44.23%), Query Frame = 0


Query: 1   MDLEQGGETAID--EILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQ 60
           M++E GG+ A+   E+LEAQ+HI +HIF+FIN MALKCAVQLGIPDAIHRHGPNPMPLSQ
Sbjct: 1   MNMELGGDIAVPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQ 60

Query: 61  LVSALQLSPQKTPHVYRLMRLL-------------------------------------- 120
           LVSALQLSPQKT ++YRLMRLL                                      
Sbjct: 61  LVSALQLSPQKTQYIYRLMRLLTHSSFFVTHKMMTKNGEEEAYVLTNSSRLLLNDNPSAV 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 TPFLLSMLEPALVEPWQLLAAWLQSEDRTPFHTTHGMPFWEFFSEKPKDGDVFNVAMASD 180

[Close]

BLAST of MC04g_new0050 vs. NCBI nr
Match: KAA0059108.1 (trans-resveratrol di-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK21637.1 trans-resveratrol di-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 187 bits (476), Expect = 5.84e-55
Identity = 125/357 (35.01%), Postives = 154/357 (43.14%), Query Frame = 0


Query: 1   MDLEQGGETAI--DEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQ 60
           M++E G + A+  DE+LEAQSHI +HIF+FIN M+LKCA+QLGIPDAIH HGPNPMPLS 
Sbjct: 1   MNMEGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 60

Query: 61  LVSALQLSPQKTPHVYRLMRLL-------------------------------------- 120
           LVS+LQL P KT  +YRLMRLL                                      
Sbjct: 61  LVSSLQLHPNKTQSIYRLMRLLTHSGFFVLKEEGYVLTNSSHLLLKDNPCTLSPFILSML 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 ESAFVEPSHFLSAWFRTDDQTPFETAHGMSFWEFVGNKQKDGDIFNAGMASDARLVMSVL 180

[Close]

BLAST of MC04g_new0050 vs. NCBI nr
Match: XP_038887584.1 (probable O-methyltransferase 3 [Benincasa hispida])

HSP 1 Score: 186 bits (471), Expect = 3.45e-54
Identity = 128/358 (35.75%), Postives = 151/358 (42.18%), Query Frame = 0


Query: 1   MDLEQGG--ETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQ 60
           M+LE G   E   DE+LEAQSHI +HIF+FIN MALKCA+QLGIPDAIH HGPNPMPLS 
Sbjct: 3   MNLEGGNMIEMGGDELLEAQSHIWNHIFNFINSMALKCAIQLGIPDAIHSHGPNPMPLSL 62

Query: 61  LVSALQLSPQKTPHVYRLMRLL-------------------------------------- 120
           L+S+LQL P K+  +YRLMRLL                                      
Sbjct: 63  LLSSLQLHPNKSQFIYRLMRLLTHSGFFILQEEGYMLTNSSRLLLKDNPYAVSPFLFCIL 122

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 123 EPTFMEPWQFLSTWLHCDDQRTPFETVHGMSFWEYIRNKHKYGDIFNASMASDTMLVVSL 182

[Close]

BLAST of MC04g_new0050 vs. ExPASy TrEMBL
Match: A0A6J1C2M7 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007812 PE=3 SV=1)

HSP 1 Score: 293 bits (751), Expect = 1.30e-96
Identity = 181/369 (49.05%), Postives = 181/369 (49.05%), Query Frame = 0


Query: 1   MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV 60
           MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV
Sbjct: 1   MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV 60

Query: 61  SALQLSPQKTPHVYRLMRLL---------------------------------------- 120
           SALQLSPQKTPHVYRLMRLL                                        
Sbjct: 61  SALQLSPQKTPHVYRLMRLLVHLGFFVMQKLSQNGEQEKEEEGYVLTNSSRFLLKDNPLT 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 LTPFVLSMLDPGLVEPWRFLSASFQSSDDHRTAFDMAHGTPFWEFMASRQKDSDVFNAGM 180

[Close]

BLAST of MC04g_new0050 vs. ExPASy TrEMBL
Match: A0A6J1C1K6 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007626 PE=3 SV=1)

HSP 1 Score: 218 bits (556), Expect = 3.42e-67
Identity = 142/366 (38.80%), Postives = 163/366 (44.54%), Query Frame = 0


Query: 1   MDLEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLV 60
           MDLEQG E A+DEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIH HGPNPMPLSQLV
Sbjct: 1   MDLEQGEEMAVDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHSHGPNPMPLSQLV 60

Query: 61  SALQLSPQKTPHVYRLMRLL---------------------------------------- 120
           SALQLSPQKTPH+YRLMRLL                                        
Sbjct: 61  SALQLSPQKTPHIYRLMRLLVHSGFFVMHKLSSQNGAEQKEEEGYVLTNSSRLFLKDNPL 120

Query: 121 -----------------------------------------W------------------ 180
                                                    W                  
Sbjct: 121 NLAPYLLYMFEPFRVEPWQFLSAWFRSEDRTPFDTAHGMSFWEYMGRKPRDSEVFNAAMA 180

[Close]

BLAST of MC04g_new0050 vs. ExPASy TrEMBL
Match: A0A6J1C1F3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE=3 SV=1)

HSP 1 Score: 200 bits (508), Expect = 5.22e-60
Identity = 132/364 (36.26%), Postives = 161/364 (44.23%), Query Frame = 0


Query: 1   MDLEQGGETAID--EILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQ 60
           M++E GG+ A+   E+LEAQ+HI +HIF+FIN MALKCAVQLGIPDAIHRHGPNPMPLSQ
Sbjct: 1   MNMELGGDIAVPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQ 60

Query: 61  LVSALQLSPQKTPHVYRLMRLL-------------------------------------- 120
           LVSALQLSPQKT ++YRLMRLL                                      
Sbjct: 61  LVSALQLSPQKTQYIYRLMRLLTHSSFFVTHKMMTKNGEEEAYVLTNSSRLLLNDNPSAV 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 TPFLLSMLEPALVEPWQLLAAWLQSEDRTPFHTTHGMPFWEFFSEKPKDGDVFNVAMASD 180

[Close]

BLAST of MC04g_new0050 vs. ExPASy TrEMBL
Match: A0A5D3DD88 (Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00380 PE=3 SV=1)

HSP 1 Score: 187 bits (476), Expect = 2.83e-55
Identity = 125/357 (35.01%), Postives = 154/357 (43.14%), Query Frame = 0


Query: 1   MDLEQGGETAI--DEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQ 60
           M++E G + A+  DE+LEAQSHI +HIF+FIN M+LKCA+QLGIPDAIH HGPNPMPLS 
Sbjct: 1   MNMEGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 60

Query: 61  LVSALQLSPQKTPHVYRLMRLL-------------------------------------- 120
           LVS+LQL P KT  +YRLMRLL                                      
Sbjct: 61  LVSSLQLHPNKTQSIYRLMRLLTHSGFFVLKEEGYVLTNSSHLLLKDNPCTLSPFILSML 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 ESAFVEPSHFLSAWFRTDDQTPFETAHGMSFWEFVGNKQKDGDIFNAGMASDARLVMSVL 180

[Close]

BLAST of MC04g_new0050 vs. ExPASy TrEMBL
Match: A0A0R0GFD5 (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_14G200800 PE=3 SV=1)

HSP 1 Score: 180 bits (457), Expect = 2.82e-53
Identity = 106/285 (37.19%), Postives = 145/285 (50.88%), Query Frame = 0


Query: 3   LEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRHGPNPMPLSQLVSA 62
           +E   E    ++L AQ+HI +HIFSFIN M+LKC V LGIPD IH +G  PMPLS L+++
Sbjct: 1   MESHDEEHAAKLLRAQTHIWNHIFSFINSMSLKCVVDLGIPDIIHNYG-QPMPLSNLIAS 60

Query: 63  LQLSPQKTPHVYRLMRLL------------------------------------------ 122
           L + P KT  V+RLMR++                                          
Sbjct: 61  LPIHPSKTCFVHRLMRIMIHSGFFSLQNHDLENELEAKYVLTDASVLLLKNHPMRSVHGM 120

Query: 123 ------------------------------------------------------------ 181
                                                                       
Sbjct: 121 ESLVDVGGGTGTMAKAIAKSFPRVECIVFDLPHVVSGLKGSENLKYVAGDMFEAIPPADA 180

[Close]

BLAST of MC04g_new0050 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.4e-16
Identity = 42/107 (39.25%), Postives = 65/107 (60.75%), Query Frame = 0


Query: 81  WVLHDWSDEGCIKILKKCKEAITEHGKKGKVMVIEMVVGNKK-------MDDKVVQTQLF 140
           WVLHDW D+ CIKILK CKEA+  +   GKV+++E V+G KK        DDK+   +L 
Sbjct: 219 WVLHDWGDKDCIKILKNCKEAVLPN--IGKVLIVECVIGEKKNTMIAEERDDKLEHVRLQ 278

Query: 141 FDILLMTMLVGGRERSEEEWAQIFYASGFSDYKIFPILGLRSLMEIY 181
            D+++M     G+ER+ +EW  +   +GF+ Y++     ++SL+  Y
Sbjct: 279 LDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

HSP 2 Score: 53.1 bits (126), Expect = 2.6e-07
Identity = 30/79 (37.97%), Postives = 44/79 (55.70%), Query Frame = 0


Query: 3  LEQGGETAIDEILEAQSHICSHIFSFINPMALKCAVQLGIPDAIHRH-GPNPMPLSQLVS 62
          +E+     +DE  +A   I  ++F F +  A KCA+ L IP+AI  H    P+ LS+L S
Sbjct: 1  MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60

Query: 63 ALQLSPQKTPHVYRLMRLL 81
          A+  SP    H+ R+MR L
Sbjct: 61 AVSASPS---HLRRIMRFL 76

[Close]

BLAST of MC04g_new0050 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 82.4 bits (202), Expect = 4.1e-16
Identity = 43/107 (40.19%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 81  WVLHDWSDEGCIKILKKCKEAITEHGKKGKVMVIEMVVG-NKK------MDDKVVQTQLF 140
           WVLHDW D+ CIKILK CKEA+  +   GKV+++E V+G NKK       D+K+   +L 
Sbjct: 276 WVLHDWGDKDCIKILKNCKEAVPPN--IGKVLIVESVIGENKKTMIVDERDEKLEHVRLM 335

Query: 141 FDILLMTMLVGGRERSEEEWAQIFYASGFSDYKIFPILGLRSLMEIY 181
            D+++M     G+ER+ +EW  +   +GF+ Y++  I  ++SL+  Y
Sbjct: 336 LDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLIIAY 380

[Close]

BLAST of MC04g_new0050 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 75.9 bits (185), Expect = 3.8e-14
Identity = 37/83 (44.58%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 81  WVLHDWSDEGCIKILKKCKEAITEHGKKGKVMVIEMVVG-NKKMDDKVVQTQLFFDILLM 140
           W+LHDW DE CIKILK C +++ E   KGKV+++EM+     K +D    T L  D+L++
Sbjct: 283 WILHDWGDEDCIKILKNCWKSLPE---KGKVIIVEMITPMEPKPNDFSCNTVLGMDLLML 342

Query: 141 TMLVGGRERSEEEWAQIFYASGF 163
           T   GG+ERS  ++  + +ASGF
Sbjct: 343 TQCSGGKERSLSQFENLAFASGF 362

[Close]

BLAST of MC04g_new0050 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 5.0e-14
Identity = 40/104 (38.46%), Postives = 67/104 (64.42%), Query Frame = 0


Query: 81  WVLHDWSDEGCIKILKKCKEAITEHGKKGKVMVIEMVVGNKKMD-DKVVQTQLFFDILLM 140
           W+LHDW+DE C++ILK CK+A+ E    G+++VIEM+V  +  + D   +  L  D+ +M
Sbjct: 264 WILHDWNDEKCVEILKNCKKALPE---TGRIIVIEMIVPREVSETDLATKNSLSADLTMM 323

Query: 141 TMLVGGRERSEEEWAQIFYASGFSDYKIFPILGLRS--LMEIYP 182
           ++  GG+ER+++E+  +   +GF   KI  I G  S  ++E+YP
Sbjct: 324 SLTSGGKERTKKEFEDLAKEAGFKLPKI--IYGAYSYWIIELYP 362

[Close]

BLAST of MC04g_new0050 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 5.0e-14
Identity = 40/104 (38.46%), Postives = 67/104 (64.42%), Query Frame = 0


Query: 81  WVLHDWSDEGCIKILKKCKEAITEHGKKGKVMVIEMVVGNKKMD-DKVVQTQLFFDILLM 140
           W+LHDW+DE C++ILK CK+A+ E    G+++VIEM+V  +  + D   +  L  D+ +M
Sbjct: 264 WILHDWNDEKCVEILKNCKKALPE---TGRIIVIEMIVPREVSETDLATKNSLSADLTMM 323

Query: 141 TMLVGGRERSEEEWAQIFYASGFSDYKIFPILGLRS--LMEIYP 182
           ++  GG+ER+++E+  +   +GF   KI  I G  S  ++E+YP
Sbjct: 324 SLTSGGKERTKKEFEDLAKEAGFKLPKI--IYGAYSYWIIELYP 362

The following BLAST results are available for this feature:

BLAST of MC04g_new0050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	3.3e-31	30.14	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	2.4e-29	27.42	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
A8QW53	5.9e-28	43.24	5-pentadecatrienyl resorcinol O-methyltransferase OS=Sorghum bicolor OX=4558 GN=...	[more]
Q6VMW0	7.2e-26	24.52	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=...	[more]
A0A0N9HMN6	9.4e-26	25.61	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]

BLAST of MC04g_new0050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136005.1	2.69e-96	49.05	probable O-methyltransferase 3 [Momordica charantia]	[more]
XP_022135736.1	7.07e-67	38.80	probable O-methyltransferase 3 [Momordica charantia]	[more]
XP_022135671.1	1.08e-59	36.26	probable O-methyltransferase 3 [Momordica charantia]	[more]
KAA0059108.1	5.84e-55	35.01	trans-resveratrol di-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK216...	[more]
XP_038887584.1	3.45e-54	35.75	probable O-methyltransferase 3 [Benincasa hispida]	[more]

BLAST of MC04g_new0050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2M7	1.30e-96	49.05	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007812 PE...	[more]
A0A6J1C1K6	3.42e-67	38.80	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007626 PE...	[more]
A0A6J1C1F3	5.22e-60	36.26	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE...	[more]
A0A5D3DD88	2.83e-55	35.01	Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A0R0GFD5	2.82e-53	37.19	Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_14G200800 PE=3 SV=1	[more]

BLAST of MC04g_new0050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35150.1	2.4e-16	39.25	O-methyltransferase family protein	[more]
AT4G35160.1	4.1e-16	40.19	O-methyltransferase family protein	[more]
AT1G77530.1	3.8e-14	44.58	O-methyltransferase family protein	[more]
AT1G51990.1	5.0e-14	38.46	O-methyltransferase family protein	[more]
AT1G51990.2	5.0e-14	38.46	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 76..163 e-value: 1.1E-19 score: 70.6
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 81..167 e-value: 3.6E-25 score: 91.0
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 79..181 coord: 11..80
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 32..80 e-value: 8.5E-17 score: 60.9
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 5..80 e-value: 1.3E-28 score: 100.7
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 79..181 coord: 11..80
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 1..181 score: 30.8081
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 74..179
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 12..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g_new0050.1	MC04g_new0050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008168	methyltransferase activity