MC04g_new0019 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g_new0019 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.3e-12
Identity = 44/138 (31.88%), Postives = 82/138 (59.42%), Query Frame = 0

Query: 2   IKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIEN 61
           +K A+SR+++LK K+ +I+R  + ++AEL+    ++ A  RVE +IRDE L+E + +IE 
Sbjct: 17  LKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECFQIIEV 76

Query: 62  FCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAAT 121
            CE +    + +      P ++ E+I +L+++S R   +PEL+ ++   + ++G+     
Sbjct: 77  LCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGKGLENE 136

Query: 122 AVELCPGNLVNPQIKEKL 140
           A   C  + VNP+I  KL
Sbjct: 137 ANCHCSTH-VNPKIVHKL 152

BLAST of MC04g_new0019 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-11
Identity = 44/162 (27.16%), Postives = 93/162 (57.41%), Query Frame = 0

Query: 2   IKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIEN 61
           ++  ++RLK+L+KK+  + ++ R+++A+ ++ G  + A  RVE +IR++ L+EA +++E 
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 62  FCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARC-GDLPELKSVRKLFEERFGRSFAA 121
           +C+ +L  F  ++  K     + E++S+LI+A+ R   ++ ELK V      ++ + +  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYG- 134

Query: 122 TAVELCPGN---LVNPQIKEKLLVKPVSDHEKQGLISEIARD 160
              +LC  N    VN ++  KL V+       +  + EIA++
Sbjct: 135 ---KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MC04g_new0019 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-11
Identity = 44/162 (27.16%), Postives = 93/162 (57.41%), Query Frame = 0

Query: 2   IKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIEN 61
           ++  ++RLK+L+KK+  + ++ R+++A+ ++ G  + A  RVE +IR++ L+EA +++E 
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 62  FCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARC-GDLPELKSVRKLFEERFGRSFAA 121
           +C+ +L  F  ++  K     + E++S+LI+A+ R   ++ ELK V      ++ + +  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYG- 134

Query: 122 TAVELCPGN---LVNPQIKEKLLVKPVSDHEKQGLISEIARD 160
              +LC  N    VN ++  KL V+       +  + EIA++
Sbjct: 135 ---KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MC04g_new0019 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-11
Identity = 44/162 (27.16%), Postives = 93/162 (57.41%), Query Frame = 0

Query: 2   IKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIEN 61
           ++  ++RLK+L+KK+  + ++ R+++A+ ++ G  + A  RVE +IR++ L+EA +++E 
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 62  FCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARC-GDLPELKSVRKLFEERFGRSFAA 121
           +C+ +L  F  ++  K     + E++S+LI+A+ R   ++ ELK V      ++ + +  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYG- 134

Query: 122 TAVELCPGN---LVNPQIKEKLLVKPVSDHEKQGLISEIARD 160
              +LC  N    VN ++  KL V+       +  + EIA++
Sbjct: 135 ---KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MC04g_new0019 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-11
Identity = 44/162 (27.16%), Postives = 93/162 (57.41%), Query Frame = 0

Query: 2   IKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIEN 61
           ++  ++RLK+L+KK+  + ++ R+++A+ ++ G  + A  RVE +IR++ L+EA +++E 
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 62  FCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARC-GDLPELKSVRKLFEERFGRSFAA 121
           +C+ +L  F  ++  K     + E++S+LI+A+ R   ++ ELK V      ++ + +  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKGYG- 134

Query: 122 TAVELCPGN---LVNPQIKEKLLVKPVSDHEKQGLISEIARD 160
              +LC  N    VN ++  KL V+       +  + EIA++
Sbjct: 135 ---KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MC04g_new0019 vs. NCBI nr
Match: XP_022147829.1 (uncharacterized protein LOC111016673 [Momordica charantia])

HSP 1 Score: 994 bits (2571), Expect = 0.0
Identity = 496/496 (100.00%), Postives = 496/496 (100.00%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE
Sbjct: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA
Sbjct: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
           TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL
Sbjct: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180

Query: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASSPECSPFCEETL 240
           ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASSPECSPFCEETL
Sbjct: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASSPECSPFCEETL 240

Query: 241 VYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVS 300
           VYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVS
Sbjct: 241 VYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVS 300

Query: 301 ERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSY 360
           ERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSY
Sbjct: 301 ERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSY 360

Query: 361 VYSEVLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRTEKIRNCEVGTIVYDVFVYSHC 420
           VYSEVLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRTEKIRNCEVGTIVYDVFVYSHC
Sbjct: 361 VYSEVLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRTEKIRNCEVGTIVYDVFVYSHC 420

Query: 421 QPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAA 480
           QPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAA
Sbjct: 421 QPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAA 480

Query: 481 KFIALKREYSQRKQHQ 496
           KFIALKREYSQRKQHQ
Sbjct: 481 KFIALKREYSQRKQHQ 496

BLAST of MC04g_new0019 vs. NCBI nr
Match: XP_038888606.1 (uncharacterized protein LOC120078409 [Benincasa hispida])

HSP 1 Score: 597 bits (1538), Expect = 7.98e-208
Identity = 328/527 (62.24%), Postives = 387/527 (73.43%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +I+ AL RL+MLKKKRYSI++QLREDL EL++NGYQQIAF RVEQLI+DE LMEAYDLIE
Sbjct: 17  LIRTALCRLQMLKKKRYSIVKQLREDLFELVNNGYQQIAFRRVEQLIQDEILMEAYDLIE 76

Query: 61  NFCEFILLNFSHVRKHK------TCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERF 120
           NFCEFIL+ FSH++KHK      TCPDD+IEAISSLIFASARCGD PELKSVRKLFE+RF
Sbjct: 77  NFCEFILVKFSHIKKHKYFMVFRTCPDDIIEAISSLIFASARCGDFPELKSVRKLFEKRF 136

Query: 121 GRSFAATAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDW 180
           GRSFA  AVELCPGNLVN QIKEKLL+KPVSDHEKQ  I++IARDCF P+ILALEY PDW
Sbjct: 137 GRSFAVAAVELCPGNLVNSQIKEKLLLKPVSDHEKQRFINDIARDCFYPSILALEYSPDW 196

Query: 181 HQKQVLENGDQTHCESKEEP-----------NIQDSNAEEFERKVMCVSPSQEVCSSSAT 240
           HQKQV +N D+T+ E KEEP           + +D+N +  + +    S S  VC SS  
Sbjct: 197 HQKQVHDNEDETNGEGKEEPEKLEKVVISLNSSRDNNRDVVDARCHSTSSSSLVCQSSPN 256

Query: 241 LE---------DASSPECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGM 300
                      DASSPE  PFC+E++VYLD++VEL + SME G   DQR FKFKS VT M
Sbjct: 257 EREDVGLDRENDASSPEILPFCDESIVYLDNIVELSDLSMEYGDSLDQRFFKFKSLVTSM 316

Query: 301 RENVQYGNGQGLIEEHHNNSKKRSVSERSSQSMNKSPK--RSMRRSTHQEDDSLSHPKKR 360
            ENV+ GN Q LIE+H ++S  + VS RS+Q +  SPK  R +     QE+ SL++ KK+
Sbjct: 317 GENVRDGNDQSLIEKH-DDSNTKLVSGRSNQIIIGSPKELRIVSMCREQENHSLNNTKKK 376

Query: 361 LMKCCCLSCHSSWLPSGMENYCLEQPSYVYSE-------VLISEKKKVDPHFILDNSLSG 420
            MKCCCLSCHS  L S ++NYCLEQP YVYSE       V  S  K  D      +S + 
Sbjct: 377 FMKCCCLSCHS--LSSELQNYCLEQPCYVYSECRTDDYVVRSSAVKHTDYDLSSKDSWNE 436

Query: 421 KDRHRVEFDTLPRTEKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSL 480
                +EFDT  RT+K  N   GT+VYDVFVYSHCQPVENKETNAKPEE+ T  K+E+S+
Sbjct: 437 DSNEEIEFDTFSRTKKASNYGNGTVVYDVFVYSHCQPVENKETNAKPEELRTNGKYETSV 496

Query: 481 GFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAAKFIALKREYSQR 492
           GFNG+++ FTKC K ADKYPSHVHPKLPDYDEIAAKFIALKREY Q+
Sbjct: 497 GFNGIQNKFTKCMKGADKYPSHVHPKLPDYDEIAAKFIALKREYLQK 540

BLAST of MC04g_new0019 vs. NCBI nr
Match: KAE8645768.1 (hypothetical protein Csa_020499 [Cucumis sativus])

HSP 1 Score: 548 bits (1412), Expect = 7.06e-186
Identity = 309/518 (59.65%), Postives = 363/518 (70.08%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +IK AL RLK+L+KKRYSI++ LREDL ELI+NGYQQIAF RVEQLI+DE+LMEAYDLIE
Sbjct: 240 LIKTALCRLKLLRKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLIQDETLMEAYDLIE 299

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           NFCE IL+ FSH+RKHKTCPDD+ EAISSLIFASAR GD PELK VRKLFEERFG+SF  
Sbjct: 300 NFCEIILVKFSHIRKHKTCPDDITEAISSLIFASARFGDFPELKCVRKLFEERFGKSFIV 359

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
            AVEL PGNLVN QIKEKL+++PV +HEKQ LI+EIARDCF PA+LALE CPDWH+KQVL
Sbjct: 360 AAVELSPGNLVNKQIKEKLVMQPVPNHEKQRLINEIARDCFHPAVLALENCPDWHKKQVL 419

Query: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVM----------------CVSPSQEVCSSSATL 240
           +NGDQT  + K E       +EE ER V+                C S S     S +  
Sbjct: 420 QNGDQTTVKGKVE-------SEELERVVISLDSTRDSNCGVLDSWCHSTSSSPSVSQSFP 479

Query: 241 EDASSPECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRE-NVQYGNG 300
            DAS+ E  PFCEE +VY DDVVEL +PS E G L DQR FKFKS +T  RE NV  G+ 
Sbjct: 480 NDASTSEFLPFCEEAMVYFDDVVELSDPSTEYGDLLDQRFFKFKSLITSTREENVGDGDD 539

Query: 301 QGLIEEHHNNSKKRSVSERSSQSMNKSPKRSMRRSTH--QEDDSLSHPKKRLMKCCCLSC 360
           Q LIE H+ ++KK +VS RS+Q +N SPK   RRS    QE+ SL+H KK+LMKC CLSC
Sbjct: 540 QSLIENHYGSNKK-AVSGRSNQIINGSPKEPSRRSMRREQENHSLNHTKKKLMKCYCLSC 599

Query: 361 HSSWLPSGMENYCLEQPSYVYSE-------VLISEKKKVDPHFILDNSLSGKDRHRVEFD 420
           HS  L S ++NYC+EQ  YV+SE       +  SE    D      N  + +    +EF 
Sbjct: 600 HS--LSSELQNYCVEQQCYVHSECRTDFSVIQSSEANNSDYDLSSKNLWNAESNEEIEFI 659

Query: 421 TLPRTEKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSF 480
           T  R +  RN  +GT+VYDVFVYS CQP ENKETN K +E+ST  KHE         S F
Sbjct: 660 TFSRAKDQRNYGIGTVVYDVFVYSQCQPDENKETNTKLKELSTNGKHEP--------SKF 719

Query: 481 TKCTKAADKYPSHVHPKLPDYDEIAAKFIALKREYSQR 492
           TKC K ADKYPSHVHPKLP+Y+EIAAKFI LKREY +R
Sbjct: 720 TKCMKGADKYPSHVHPKLPNYEEIAAKFICLKREYLER 739

BLAST of MC04g_new0019 vs. NCBI nr
Match: XP_016901704.1 (PREDICTED: uncharacterized protein LOC103495691 [Cucumis melo])

HSP 1 Score: 521 bits (1341), Expect = 2.09e-178
Identity = 303/513 (59.06%), Postives = 352/513 (68.62%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +IK AL RLK+LKKKRYSI++ LREDL ELI+NGYQQIAF RVEQL++DE+LME YDLIE
Sbjct: 17  LIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDLIE 76

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           N CEFIL+ FSHVRKHKTCPDDVIEAISSLIFASAR GD PELK VR LFEERFG+SFA 
Sbjct: 77  NLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSFAV 136

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
            AVEL PGNLVN QIKEKL+++ VS+HEKQ LI+EIARDCF PA+LALEY PD  QKQVL
Sbjct: 137 AAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQVL 196

Query: 181 ENGDQTHCESKEEP-----------NIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASS 240
           +N DQT  E K E            + +DSN    +      S S  VC S     DASS
Sbjct: 197 QNEDQTTVEGKVESEELERVVISLDSARDSNCGVLDPWCHSTSSSSSVCQSFPN--DASS 256

Query: 241 PECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRE-NVQYGNGQGLIE 300
            E  PFCEE +VY DDVVEL +PS E G L DQR FKFK  +T  RE NV+  + Q LIE
Sbjct: 257 SEFLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIE 316

Query: 301 EHHNNSKKRSVSERSSQSMNKSPKRSMRR--STHQEDDSLSHPKKRLMKCCCLSCHSSWL 360
           +H + S K+SVS RS+Q +N  PK   R+     QE+ SL+H KK+  KCCCLSCHS  L
Sbjct: 317 KH-DASNKKSVSGRSNQIINGPPKEPSRKFMCREQENHSLNHTKKKFTKCCCLSCHS--L 376

Query: 361 PSGMENYCLEQPSYVYSE-------VLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRT 420
              ++NY LEQ  YVYSE       +  SE  ++D      +  + +    +EF T  R 
Sbjct: 377 SFELQNYSLEQQCYVYSECRTDYYIIRSSEANRLDYDLSSKHLWNAESNEEIEFITFSRA 436

Query: 421 EKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTK 480
           ++ RN  +GT+VYDVFVYSHCQP ENKETN K EE      HE         S FTKC K
Sbjct: 437 KEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEE------HEP--------SKFTKCMK 496

Query: 481 AADKYPSHVHPKLPDYDEIAAKFIALKREYSQR 492
            A KYPSHVHPKLPDYDEIA +FI+LKREY QR
Sbjct: 497 GAHKYPSHVHPKLPDYDEIAVEFISLKREYLQR 510

BLAST of MC04g_new0019 vs. NCBI nr
Match: KAA0031582.1 (Ist1 domain-containing protein [Cucumis melo var. makuwa] >TYK07034.1 Ist1 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 520 bits (1338), Expect = 4.08e-178
Identity = 303/513 (59.06%), Postives = 351/513 (68.42%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +IK AL RLK+LKKKRYSI++ LREDL ELI+NGYQQIAF RVEQL++DE+LME YDLIE
Sbjct: 6   LIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDLIE 65

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           N CEFIL+ FSHVRKHKTCPDDVIEAISSLIFASAR GD PELK VR LFEERFG+SFA 
Sbjct: 66  NLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSFAV 125

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
            AVEL PGNLVN QIKEKL+++ VS+HEKQ LI+EIARDCF PA+LALEY PD  QKQVL
Sbjct: 126 AAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQVL 185

Query: 181 ENGDQTHCESKEEP-----------NIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASS 240
            N DQT  E K E            + +DSN    +      S S  VC S     DASS
Sbjct: 186 PNEDQTTVEGKVESEELERVVISLDSARDSNCGVLDPWCHSTSSSSSVCQSFPN--DASS 245

Query: 241 PECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRE-NVQYGNGQGLIE 300
            E  PFCEE +VY DDVVEL +PS E G L DQR FKFK  +T  RE NV+  + Q LIE
Sbjct: 246 SEFLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIE 305

Query: 301 EHHNNSKKRSVSERSSQSMNKSPKRSMRR--STHQEDDSLSHPKKRLMKCCCLSCHSSWL 360
           +H + S K+SVS RS+Q +N  PK   R+     QE+ SL+H KK+  KCCCLSCHS  L
Sbjct: 306 KH-DASNKKSVSGRSNQIINGPPKEPSRKLMCREQENHSLNHTKKKFTKCCCLSCHS--L 365

Query: 361 PSGMENYCLEQPSYVYSE-------VLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRT 420
              ++NY LEQ  YVYSE       +  SE  ++D      +  + +    +EF T  R 
Sbjct: 366 SFELQNYSLEQQCYVYSECRTDYYIIRSSEANRLDYDLSSKHLWNAESNEEIEFITFSRA 425

Query: 421 EKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTK 480
           ++ RN  +GT+VYDVFVYSHCQP ENKETN K EE      HE         S FTKC K
Sbjct: 426 KEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEE------HEP--------SKFTKCMK 485

Query: 481 AADKYPSHVHPKLPDYDEIAAKFIALKREYSQR 492
            A KYPSHVHPKLPDYDEIA +FI+LKREY QR
Sbjct: 486 GAHKYPSHVHPKLPDYDEIAVEFISLKREYLQR 499

BLAST of MC04g_new0019 vs. ExPASy TrEMBL
Match: A0A6J1D262 (uncharacterized protein LOC111016673 OS=Momordica charantia OX=3673 GN=LOC111016673 PE=3 SV=1)

HSP 1 Score: 994 bits (2571), Expect = 0.0
Identity = 496/496 (100.00%), Postives = 496/496 (100.00%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE
Sbjct: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA
Sbjct: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
           TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL
Sbjct: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180

Query: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASSPECSPFCEETL 240
           ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASSPECSPFCEETL
Sbjct: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASSPECSPFCEETL 240

Query: 241 VYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVS 300
           VYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVS
Sbjct: 241 VYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRSVS 300

Query: 301 ERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSY 360
           ERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSY
Sbjct: 301 ERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQPSY 360

Query: 361 VYSEVLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRTEKIRNCEVGTIVYDVFVYSHC 420
           VYSEVLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRTEKIRNCEVGTIVYDVFVYSHC
Sbjct: 361 VYSEVLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRTEKIRNCEVGTIVYDVFVYSHC 420

Query: 421 QPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAA 480
           QPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAA
Sbjct: 421 QPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAA 480

Query: 481 KFIALKREYSQRKQHQ 496
           KFIALKREYSQRKQHQ
Sbjct: 481 KFIALKREYSQRKQHQ 496

BLAST of MC04g_new0019 vs. ExPASy TrEMBL
Match: A0A0A0K3R2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G047390 PE=3 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 2.57e-189
Identity = 309/518 (59.65%), Postives = 363/518 (70.08%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +IK AL RLK+L+KKRYSI++ LREDL ELI+NGYQQIAF RVEQLI+DE+LMEAYDLIE
Sbjct: 22  LIKTALCRLKLLRKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLIQDETLMEAYDLIE 81

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           NFCE IL+ FSH+RKHKTCPDD+ EAISSLIFASAR GD PELK VRKLFEERFG+SF  
Sbjct: 82  NFCEIILVKFSHIRKHKTCPDDITEAISSLIFASARFGDFPELKCVRKLFEERFGKSFIV 141

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
            AVEL PGNLVN QIKEKL+++PV +HEKQ LI+EIARDCF PA+LALE CPDWH+KQVL
Sbjct: 142 AAVELSPGNLVNKQIKEKLVMQPVPNHEKQRLINEIARDCFHPAVLALENCPDWHKKQVL 201

Query: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVM----------------CVSPSQEVCSSSATL 240
           +NGDQT  + K E       +EE ER V+                C S S     S +  
Sbjct: 202 QNGDQTTVKGKVE-------SEELERVVISLDSTRDSNCGVLDSWCHSTSSSPSVSQSFP 261

Query: 241 EDASSPECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRE-NVQYGNG 300
            DAS+ E  PFCEE +VY DDVVEL +PS E G L DQR FKFKS +T  RE NV  G+ 
Sbjct: 262 NDASTSEFLPFCEEAMVYFDDVVELSDPSTEYGDLLDQRFFKFKSLITSTREENVGDGDD 321

Query: 301 QGLIEEHHNNSKKRSVSERSSQSMNKSPKRSMRRSTH--QEDDSLSHPKKRLMKCCCLSC 360
           Q LIE H+ ++KK +VS RS+Q +N SPK   RRS    QE+ SL+H KK+LMKC CLSC
Sbjct: 322 QSLIENHYGSNKK-AVSGRSNQIINGSPKEPSRRSMRREQENHSLNHTKKKLMKCYCLSC 381

Query: 361 HSSWLPSGMENYCLEQPSYVYSE-------VLISEKKKVDPHFILDNSLSGKDRHRVEFD 420
           HS  L S ++NYC+EQ  YV+SE       +  SE    D      N  + +    +EF 
Sbjct: 382 HS--LSSELQNYCVEQQCYVHSECRTDFSVIQSSEANNSDYDLSSKNLWNAESNEEIEFI 441

Query: 421 TLPRTEKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSF 480
           T  R +  RN  +GT+VYDVFVYS CQP ENKETN K +E+ST  KHE         S F
Sbjct: 442 TFSRAKDQRNYGIGTVVYDVFVYSQCQPDENKETNTKLKELSTNGKHEP--------SKF 501

Query: 481 TKCTKAADKYPSHVHPKLPDYDEIAAKFIALKREYSQR 492
           TKC K ADKYPSHVHPKLP+Y+EIAAKFI LKREY +R
Sbjct: 502 TKCMKGADKYPSHVHPKLPNYEEIAAKFICLKREYLER 521

BLAST of MC04g_new0019 vs. ExPASy TrEMBL
Match: A0A1S4E0G2 (uncharacterized protein LOC103495691 OS=Cucumis melo OX=3656 GN=LOC103495691 PE=3 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 1.01e-178
Identity = 303/513 (59.06%), Postives = 352/513 (68.62%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +IK AL RLK+LKKKRYSI++ LREDL ELI+NGYQQIAF RVEQL++DE+LME YDLIE
Sbjct: 17  LIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDLIE 76

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           N CEFIL+ FSHVRKHKTCPDDVIEAISSLIFASAR GD PELK VR LFEERFG+SFA 
Sbjct: 77  NLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSFAV 136

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
            AVEL PGNLVN QIKEKL+++ VS+HEKQ LI+EIARDCF PA+LALEY PD  QKQVL
Sbjct: 137 AAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQVL 196

Query: 181 ENGDQTHCESKEEP-----------NIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASS 240
           +N DQT  E K E            + +DSN    +      S S  VC S     DASS
Sbjct: 197 QNEDQTTVEGKVESEELERVVISLDSARDSNCGVLDPWCHSTSSSSSVCQSFPN--DASS 256

Query: 241 PECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRE-NVQYGNGQGLIE 300
            E  PFCEE +VY DDVVEL +PS E G L DQR FKFK  +T  RE NV+  + Q LIE
Sbjct: 257 SEFLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIE 316

Query: 301 EHHNNSKKRSVSERSSQSMNKSPKRSMRR--STHQEDDSLSHPKKRLMKCCCLSCHSSWL 360
           +H + S K+SVS RS+Q +N  PK   R+     QE+ SL+H KK+  KCCCLSCHS  L
Sbjct: 317 KH-DASNKKSVSGRSNQIINGPPKEPSRKFMCREQENHSLNHTKKKFTKCCCLSCHS--L 376

Query: 361 PSGMENYCLEQPSYVYSE-------VLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRT 420
              ++NY LEQ  YVYSE       +  SE  ++D      +  + +    +EF T  R 
Sbjct: 377 SFELQNYSLEQQCYVYSECRTDYYIIRSSEANRLDYDLSSKHLWNAESNEEIEFITFSRA 436

Query: 421 EKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTK 480
           ++ RN  +GT+VYDVFVYSHCQP ENKETN K EE      HE         S FTKC K
Sbjct: 437 KEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEE------HEP--------SKFTKCMK 496

Query: 481 AADKYPSHVHPKLPDYDEIAAKFIALKREYSQR 492
            A KYPSHVHPKLPDYDEIA +FI+LKREY QR
Sbjct: 497 GAHKYPSHVHPKLPDYDEIAVEFISLKREYLQR 510

BLAST of MC04g_new0019 vs. ExPASy TrEMBL
Match: A0A5D3C6Z3 (Ist1 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003520 PE=3 SV=1)

HSP 1 Score: 520 bits (1338), Expect = 1.98e-178
Identity = 303/513 (59.06%), Postives = 351/513 (68.42%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +IK AL RLK+LKKKRYSI++ LREDL ELI+NGYQQIAF RVEQL++DE+LME YDLIE
Sbjct: 6   LIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDLIE 65

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           N CEFIL+ FSHVRKHKTCPDDVIEAISSLIFASAR GD PELK VR LFEERFG+SFA 
Sbjct: 66  NLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSFAV 125

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
            AVEL PGNLVN QIKEKL+++ VS+HEKQ LI+EIARDCF PA+LALEY PD  QKQVL
Sbjct: 126 AAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQVL 185

Query: 181 ENGDQTHCESKEEP-----------NIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASS 240
            N DQT  E K E            + +DSN    +      S S  VC S     DASS
Sbjct: 186 PNEDQTTVEGKVESEELERVVISLDSARDSNCGVLDPWCHSTSSSSSVCQSFPN--DASS 245

Query: 241 PECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRE-NVQYGNGQGLIE 300
            E  PFCEE +VY DDVVEL +PS E G L DQR FKFK  +T  RE NV+  + Q LIE
Sbjct: 246 SEFLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIE 305

Query: 301 EHHNNSKKRSVSERSSQSMNKSPKRSMRR--STHQEDDSLSHPKKRLMKCCCLSCHSSWL 360
           +H + S K+SVS RS+Q +N  PK   R+     QE+ SL+H KK+  KCCCLSCHS  L
Sbjct: 306 KH-DASNKKSVSGRSNQIINGPPKEPSRKLMCREQENHSLNHTKKKFTKCCCLSCHS--L 365

Query: 361 PSGMENYCLEQPSYVYSE-------VLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRT 420
              ++NY LEQ  YVYSE       +  SE  ++D      +  + +    +EF T  R 
Sbjct: 366 SFELQNYSLEQQCYVYSECRTDYYIIRSSEANRLDYDLSSKHLWNAESNEEIEFITFSRA 425

Query: 421 EKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTK 480
           ++ RN  +GT+VYDVFVYSHCQP ENKETN K EE      HE         S FTKC K
Sbjct: 426 KEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEE------HEP--------SKFTKCMK 485

Query: 481 AADKYPSHVHPKLPDYDEIAAKFIALKREYSQR 492
            A KYPSHVHPKLPDYDEIA +FI+LKREY QR
Sbjct: 486 GAHKYPSHVHPKLPDYDEIAVEFISLKREYLQR 499

BLAST of MC04g_new0019 vs. ExPASy TrEMBL
Match: A0A0A0KEA6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G128620 PE=3 SV=1)

HSP 1 Score: 305 bits (780), Expect = 1.18e-94
Identity = 208/492 (42.28%), Postives = 281/492 (57.11%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           +IK+   RLK+LK K+  I +QLRED+ +L+ NGY QIAFNRVEQ+++DE+ M AY++++
Sbjct: 18  LIKQVQCRLKLLKNKKSVITKQLREDIVQLLQNGYHQIAFNRVEQIVKDETRMSAYEILD 77

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           NFCEFILLN S++RKHK CP+DV EA+SSL+FASARCGDLPEL+ +RKLF ER+GRSF  
Sbjct: 78  NFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPELQLIRKLFGERYGRSFET 137

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180
           TAVEL PGNLVN QIK+KL +  VSD EKQ +++EI RDC +P +LALEY  +WHQ QV 
Sbjct: 138 TAVELNPGNLVNLQIKQKLSINYVSDDEKQRMMNEIVRDCLKPEVLALEYRSEWHQNQVT 197

Query: 181 -ENGDQTHCESK-EEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLEDASSPECSPFCEE 240
            +   Q H E K ++   Q  NA  +E K   +  S  V S+S        P+     EE
Sbjct: 198 AKEVIQVHAEEKIKQHKKQAMNA--YETKKGDIHYSNSVTSTSCEF----FPQLP---EE 257

Query: 241 TLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNNSKKRS 300
            +VYLDDVVEL + +  EG   DQRLFKFK+  T     +   N Q  I+   + S    
Sbjct: 258 RIVYLDDVVELCSSTTTEG---DQRLFKFKTTPTLSNREISKENHQNQIDLVQSESWSED 317

Query: 301 VSERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENYCLEQP 360
            +  S  S+  S KR M       +    +PKK   K      +SSW    M+ Y     
Sbjct: 318 ENSSSRTSIEGSKKRFM-------EVVEGNPKKEDYK----QENSSWKQRTMDKY----- 377

Query: 361 SYVYSEVLISEKKKVDPHFILDNSLSGKDRHRVEFDTLPRTEKIRNCEVG--TIVYDVFV 420
            +  +  + ++K+    +F        + R     DT P  +     + G    +    V
Sbjct: 378 -WASASEVTTDKEIEWANFY---KKPRRRRRTKRGDTPPSHDMKFTTDDGFNANINHKKV 437

Query: 421 YSHCQPVENKETNAKPEEISTKCKHESSLGFNGMKSSFTKCTKAADKYPSHVHPKLPDYD 480
            ++C+ V+ K+       ++   +        G   SF +      K PSHVHPKLPDYD
Sbjct: 438 EANCEKVDVKKDGLCLRAVTMPTERPKERLKEG---SFGRTKSCPYKQPSHVHPKLPDYD 474

Query: 481 EIAAKFIALKRE 488
           +IAAKFIALKRE
Sbjct: 498 DIAAKFIALKRE 474

BLAST of MC04g_new0019 vs. TAIR 10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 164.9 bits (416), Expect = 1.7e-40
Identity = 119/348 (34.20%), Postives = 175/348 (50.29%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           ++K+   RL +LK K+Y+I   LR D+A+L+  G +  A +R +QL  DE+LM  Y L+ 
Sbjct: 87  VVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRAQQLFLDENLMSLYHLLL 146

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
           +F + ILLN S++R+ +  PD + EA+S+L+FASARCGDLPEL+++R LF +R+G  F  
Sbjct: 147 HFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPELRALRVLFGKRYGNHFVD 206

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDC-FRPAILALEYCPDWHQKQV 180
           TA+ L PGN VNPQ+ EKL +  VSD  K  L+ EI  +   R  +LA+EY P++H KQV
Sbjct: 207 TALNLLPGNCVNPQVIEKLSIISVSDDAKSKLLGEIVEEYNLRLEVLAIEYTPEFH-KQV 266

Query: 181 LENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSA----------TLEDASS 240
           L+                 S   E E++VM  + +Q  CSS            TL DA  
Sbjct: 267 LK-----------------SEKAEEEKEVMSSNSAQPYCSSQKAESEAEVYKFTLTDADI 326

Query: 241 PECSPFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEE 300
            E      +     DD +E      EE    DQ +F+F+                   E+
Sbjct: 327 EENQEQERKVCDEEDDCIE------EEVVEEDQSVFRFREST----------------ED 386

Query: 301 HHNNSKKRSVSERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCC 338
                K+     RS+ S +    + +    +      S  +K   KCC
Sbjct: 387 ERKERKRLRRKPRSTSSSSSPIAKDVDCWRYYYKGKRSRQRKECGKCC 394

BLAST of MC04g_new0019 vs. TAIR 10
Match: AT1G51900.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.8e-27
Identity = 80/230 (34.78%), Postives = 128/230 (55.65%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           ++K+  SRL +LK ++Y+  R LR D+ + I +   + A  R EQL+  E+ +  Y  + 
Sbjct: 19  VLKQLQSRLMLLKSQKYAKSRHLRADIVDFIRSNDSKSALFRTEQLLLVENAITIYGFLL 78

Query: 61  NFCEFILLNFSHVRKHK--TCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSF 120
            F +FILL FS  +KH      DD  EA+SSLIFAS +C ++PEL  + +L  +R+G+ +
Sbjct: 79  KFTDFILLRFSPSKKHSCLLVNDDTSEAVSSLIFASVKCREIPELLIISELVGQRYGQRY 138

Query: 121 AATAVELCPGNLVNPQIKEKL-LVKPVSDHEKQGLISEIARDC-FRPAILALEYCPDWHQ 180
             TA+++ PGNLVN +IKEKL     VS+ +K  ++ EIA++  +R  IL L Y      
Sbjct: 139 VTTAIQVPPGNLVNTEIKEKLKSTSVVSETDKCRVMEEIAKESGYRLEILGLGY------ 198

Query: 181 KQVLENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPSQEVCSSSATLED 227
           K  ++N         EE N+ D +  E  +   C++   E      +++D
Sbjct: 199 KSEIDN----EVFDLEEKNVMDESLHEVYK--FCLTDVDERSKKETSMKD 236

HSP 2 Score: 40.0 bits (92), Expect = 6.3e-03
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 0

Query: 467 HVHPKLPDYDEIAAKFIALKREYSQRKQ 495
           HVHPKLPDYD+IA KF  LK    +R++
Sbjct: 739 HVHPKLPDYDQIATKFKLLKEVEKERRR 766

BLAST of MC04g_new0019 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 120.6 bits (301), Expect = 3.7e-27
Identity = 58/157 (36.94%), Postives = 101/157 (64.33%), Query Frame = 0

Query: 3   KRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIENF 62
           K A++R+K+++ KR  +++Q+R D+A L+ +G    A  RVE +IR++++  A ++IE F
Sbjct: 36  KMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAANEIIELF 95

Query: 63  CEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAATA 122
           CE I+   + + K K CP D+ E I+SLIFA+ RC ++PEL  +R +F +++G+ F + A
Sbjct: 96  CELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYGKDFVSAA 155

Query: 123 VELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARD 160
            +L P   VN  + +KL V+      K  ++ EIA++
Sbjct: 156 TDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKE 192

BLAST of MC04g_new0019 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 119.4 bits (298), Expect = 8.2e-27
Identity = 60/159 (37.74%), Postives = 100/159 (62.89%), Query Frame = 0

Query: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60
           ++K  + R+K+++ +R + ++Q+R ++A+L+  G +  A  RVE +IR+E +M A +++E
Sbjct: 20  LLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMMAAQEILE 79

Query: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120
            FCE I +    +   + CP D+ EAISS+ FA+ RC DL EL+ V+ LF  ++G+ F A
Sbjct: 80  LFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSKYGKEFVA 139

Query: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARD 160
            A EL P + VN ++ E L V+  S   K  L+ EIA +
Sbjct: 140 AASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEE 178

BLAST of MC04g_new0019 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 114.0 bits (284), Expect = 3.4e-25
Identity = 59/158 (37.34%), Postives = 97/158 (61.39%), Query Frame = 0

Query: 2   IKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIEN 61
           ++ A SRLK+LK K+   ++QLR +LA+L+ +G    A  RVE ++R+E  + AY+LI  
Sbjct: 18  LQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEKTVAAYELIGI 77

Query: 62  FCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAAT 121
           +CE +++    +   K CP D+ EA++S++FAS R  D+PEL  + K F  ++G+ F+ +
Sbjct: 78  YCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFTTKYGKDFSTS 137

Query: 122 AVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARD 160
           AVEL P + V+  + EKL  K      K  ++  IA +
Sbjct: 138 AVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEE 175