MC04g1844 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g1844
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionLaccase
LocationMC04: 25648087 .. 25650443 (+)
RNA-Seq ExpressionMC04g1844
SyntenyMC04g1844
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTTTACTTGTTTGGCGTTTTGCTTCTGTTTGCTTCGGCTGTGTGCTTGGCCCACCCACAAACTCACCGCCATCAGTTTGTTGTATGTTTTTTTTTTTTTTTATAAATATTATTGGCTAATTTTAATTTGCTAGTTTTCTTTTATTAATGGGTTGATTGCATTCAAAATGGAACTAACTGCAGATTCAAGCTACGCCAGTGAAGAGGCTGTGCAAAACCCACAATGCCATTACGGTCAACGGGCAGTTCCCCGGGCCGACCTTGGAGATCAGCAATGGCGACACTCTCACAGTCACTGTCATTAACAAGGCCAAGTACAACGTCACAATTCACTGGTAATTAATTAACATTTCTAATATTTAGACTCTTTTTTTTTCCCTTTTATTTGTGATTTGAATGTTGATTATATTTTTCTAAGATAAAAGAGTGTTATTTCTTATTGGACTTGCAGGCATGGAATCAGGCAGTTTAGAACCGGGTGGGCTGATGGACCCGAGTTTGTGACTCAATGCCCAATCAGACCGGGAGGAAGCTATACTTACCGGTAGATAATTTACCATTAAATTCTAAAACTTAATCTAATAATATTTAATTAAGTCTATAAGATTATAGAAAATTTAATATTTTTAATAAATTTGTAATGCAGATTTACTGTGCAAGGACAAGAAGGAACTTTGTGGTGGCATGCTCACAGCTCATGGCTTCGAGCCACGGTTTACGGTGCCTTAATCATCCGCCCCAGAGAGGGAGATTCCTACCCTTTCACCAAACCAAACCGCGAAACAACACTTCTTATGGGTAATCATAATAATAATAAAATCACATTACATTATACGATTAATAATCACACACTAAAAATACAAACATTATTACAAGTTTTAATTATTATTATTATTTTTTTTTGGTAATTATTAGGCGAATGGTGGGATGCGAATCCTATTGACGTTGTGAGGGAGGCCACTAGAACAGGAGCGGCTCCCAATATCTCTGATGCATACACATTCAATGGTCAACCGGGTGACCTTTACAAGTGTTCTAGCAAAGGTAAATAAAAATTTGCGCCACAAATGGGAATTATGAAAATTAGAAAAACAAATATTGTAATTATGGGATGACATTTGTTTTGTGTGATTAATTATGCAGATACCGTAATCGTCCCGATAGATTCCGGCGAGACGAACCTTCTCCGAGTGGTGAACTCTGCCCTGAACAATGCTCTGTTTTTCACGGTGGCGAACCACAAGCTGACGGTGGTCGGAGCCGACGCCTCATACACCAAGCCGTTCACCACCTCTGTTCTGATGCTCGGCCCTGGCCAGACCACCGACGTTTTGATCTCCGGCAACCAGGTGCCCACACGGTACTACATAGCCGCACGTGCGTATCAAAGCGCTCAGAACGCACCGTTTGACAACACCACCACCACCGCCATTCTCGAGTACAAATCCGCCCCCTGCCCCGCCAAGAAAGGCGGCGCGTCGATCAGACCAATCATGCCGTCTCTGCCGGCCTACAATGACACCGCCACCGTCACCGCCTTCAGCCGGAGCTTCCGCAGCCCGAGACCGGTGGAGGTTCCGACCGAGATCGACGAGAATCTATTTGTCACGATCGGTTTAGGACTCAACAACTGCCCGCGTAATTTCAAATCGAGCCAATGCCAAGGCCCCAACGGAACTCGCTTCACGGCTAGTATGAACAACGTCTCCTTCGTGCTTCCTTCCAACATCTCGATTCTTCAGGCCTTCCAGCAGAGAATTCCCGGCGTCATCACCCCTGATTTTCCGGCCAACCCGCCGGTGAAATTCGATTATACCGGCAACGTCAGCCGATCGCTTTTCCAACCGGTTCCCGGTACTAAAGGCTACCGATTGAAATACGGATCGAGAGTTCAGGTTGTGTTGCAGGACACGAGCATTGTGACGCCGGAAAATCACCCGATTCATCTCCATGGATACGATTTCTACATCGTTGCCGAGGGATTTGGAAACTTTAACCCGAAAACTGATACTAAAAAGTTCAACCTCGTTGACCCACCTCTCAGAAACACGGTGGCCGTGCCCGTCAATGGCTGGGCTGTCATCCGATTCGTCGCCGATAACCCAGGTAATAAATCCCAAAATTTAATTAATAAATTTAATCCTATCTAATTTCTTATTTTCAAATTTTCAATTTAGTTTAACCTAATTAAAAAGATTAAAACTCTAATAATTTTATGATGTATCAGGTGCTTGGATAATGCACTGTCACTTGGACGTGCATATCACGTGGGGGCTGGCGATGGTGTTTCTGGTAGATAATGGCGTCGGACAATTGCAGTCCATAGAGGCTCCGCCGGCGGATCTTCCGATATGC

mRNA sequence

TCTCTTTACTTGTTTGGCGTTTTGCTTCTGTTTGCTTCGGCTGTGTGCTTGGCCCACCCACAAACTCACCGCCATCAGTTTGTTATTCAAGCTACGCCAGTGAAGAGGCTGTGCAAAACCCACAATGCCATTACGGTCAACGGGCAGTTCCCCGGGCCGACCTTGGAGATCAGCAATGGCGACACTCTCACAGTCACTGTCATTAACAAGGCCAAGTACAACGTCACAATTCACTGGCATGGAATCAGGCAGTTTAGAACCGGGTGGGCTGATGGACCCGAGTTTGTGACTCAATGCCCAATCAGACCGGGAGGAAGCTATACTTACCGATTTACTGTGCAAGGACAAGAAGGAACTTTGTGGTGGCATGCTCACAGCTCATGGCTTCGAGCCACGGTTTACGGTGCCTTAATCATCCGCCCCAGAGAGGGAGATTCCTACCCTTTCACCAAACCAAACCGCGAAACAACACTTCTTATGGGCGAATGGTGGGATGCGAATCCTATTGACGTTGTGAGGGAGGCCACTAGAACAGGAGCGGCTCCCAATATCTCTGATGCATACACATTCAATGGTCAACCGGGTGACCTTTACAAGTGTTCTAGCAAAGATACCGTAATCGTCCCGATAGATTCCGGCGAGACGAACCTTCTCCGAGTGGTGAACTCTGCCCTGAACAATGCTCTGTTTTTCACGGTGGCGAACCACAAGCTGACGGTGGTCGGAGCCGACGCCTCATACACCAAGCCGTTCACCACCTCTGTTCTGATGCTCGGCCCTGGCCAGACCACCGACGTTTTGATCTCCGGCAACCAGGTGCCCACACGGTACTACATAGCCGCACGTGCGTATCAAAGCGCTCAGAACGCACCGTTTGACAACACCACCACCACCGCCATTCTCGAGTACAAATCCGCCCCCTGCCCCGCCAAGAAAGGCGGCGCGTCGATCAGACCAATCATGCCGTCTCTGCCGGCCTACAATGACACCGCCACCGTCACCGCCTTCAGCCGGAGCTTCCGCAGCCCGAGACCGGTGGAGGTTCCGACCGAGATCGACGAGAATCTATTTGTCACGATCGGTTTAGGACTCAACAACTGCCCGCGTAATTTCAAATCGAGCCAATGCCAAGGCCCCAACGGAACTCGCTTCACGGCTAGTATGAACAACGTCTCCTTCGTGCTTCCTTCCAACATCTCGATTCTTCAGGCCTTCCAGCAGAGAATTCCCGGCGTCATCACCCCTGATTTTCCGGCCAACCCGCCGGTGAAATTCGATTATACCGGCAACGTCAGCCGATCGCTTTTCCAACCGGTTCCCGGTACTAAAGGCTACCGATTGAAATACGGATCGAGAGTTCAGGTTGTGTTGCAGGACACGAGCATTGTGACGCCGGAAAATCACCCGATTCATCTCCATGGATACGATTTCTACATCGTTGCCGAGGGATTTGGAAACTTTAACCCGAAAACTGATACTAAAAAGTTCAACCTCGTTGACCCACCTCTCAGAAACACGGTGGCCGTGCCCGTCAATGGCTGGGCTGTCATCCGATTCGTCGCCGATAACCCAGGTGCTTGGATAATGCACTGTCACTTGGACGTGCATATCACGTGGGGGCTGGCGATGGTGTTTCTGGTAGATAATGGCGTCGGACAATTGCAGTCCATAGAGGCTCCGCCGGCGGATCTTCCGATATGC

Coding sequence (CDS)

TCTCTTTACTTGTTTGGCGTTTTGCTTCTGTTTGCTTCGGCTGTGTGCTTGGCCCACCCACAAACTCACCGCCATCAGTTTGTTATTCAAGCTACGCCAGTGAAGAGGCTGTGCAAAACCCACAATGCCATTACGGTCAACGGGCAGTTCCCCGGGCCGACCTTGGAGATCAGCAATGGCGACACTCTCACAGTCACTGTCATTAACAAGGCCAAGTACAACGTCACAATTCACTGGCATGGAATCAGGCAGTTTAGAACCGGGTGGGCTGATGGACCCGAGTTTGTGACTCAATGCCCAATCAGACCGGGAGGAAGCTATACTTACCGATTTACTGTGCAAGGACAAGAAGGAACTTTGTGGTGGCATGCTCACAGCTCATGGCTTCGAGCCACGGTTTACGGTGCCTTAATCATCCGCCCCAGAGAGGGAGATTCCTACCCTTTCACCAAACCAAACCGCGAAACAACACTTCTTATGGGCGAATGGTGGGATGCGAATCCTATTGACGTTGTGAGGGAGGCCACTAGAACAGGAGCGGCTCCCAATATCTCTGATGCATACACATTCAATGGTCAACCGGGTGACCTTTACAAGTGTTCTAGCAAAGATACCGTAATCGTCCCGATAGATTCCGGCGAGACGAACCTTCTCCGAGTGGTGAACTCTGCCCTGAACAATGCTCTGTTTTTCACGGTGGCGAACCACAAGCTGACGGTGGTCGGAGCCGACGCCTCATACACCAAGCCGTTCACCACCTCTGTTCTGATGCTCGGCCCTGGCCAGACCACCGACGTTTTGATCTCCGGCAACCAGGTGCCCACACGGTACTACATAGCCGCACGTGCGTATCAAAGCGCTCAGAACGCACCGTTTGACAACACCACCACCACCGCCATTCTCGAGTACAAATCCGCCCCCTGCCCCGCCAAGAAAGGCGGCGCGTCGATCAGACCAATCATGCCGTCTCTGCCGGCCTACAATGACACCGCCACCGTCACCGCCTTCAGCCGGAGCTTCCGCAGCCCGAGACCGGTGGAGGTTCCGACCGAGATCGACGAGAATCTATTTGTCACGATCGGTTTAGGACTCAACAACTGCCCGCGTAATTTCAAATCGAGCCAATGCCAAGGCCCCAACGGAACTCGCTTCACGGCTAGTATGAACAACGTCTCCTTCGTGCTTCCTTCCAACATCTCGATTCTTCAGGCCTTCCAGCAGAGAATTCCCGGCGTCATCACCCCTGATTTTCCGGCCAACCCGCCGGTGAAATTCGATTATACCGGCAACGTCAGCCGATCGCTTTTCCAACCGGTTCCCGGTACTAAAGGCTACCGATTGAAATACGGATCGAGAGTTCAGGTTGTGTTGCAGGACACGAGCATTGTGACGCCGGAAAATCACCCGATTCATCTCCATGGATACGATTTCTACATCGTTGCCGAGGGATTTGGAAACTTTAACCCGAAAACTGATACTAAAAAGTTCAACCTCGTTGACCCACCTCTCAGAAACACGGTGGCCGTGCCCGTCAATGGCTGGGCTGTCATCCGATTCGTCGCCGATAACCCAGGTGCTTGGATAATGCACTGTCACTTGGACGTGCATATCACGTGGGGGCTGGCGATGGTGTTTCTGGTAGATAATGGCGTCGGACAATTGCAGTCCATAGAGGCTCCGCCGGCGGATCTTCCGATATGC

Protein sequence

SLYLFGVLLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAMVFLVDNGVGQLQSIEAPPADLPIC
Homology
BLAST of MC04g1844 vs. ExPASy Swiss-Prot
Match: Q9SIY8 (Laccase-5 OS=Arabidopsis thaliana OX=3702 GN=LAC5 PE=2 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 5.7e-271
Identity = 438/570 (76.84%), Postives = 487/570 (85.44%), Query Frame = 0

Query: 5   FGVLLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLT 64
           F   LLF+S       + H H+F+IQAT VKRLC+THN+ITVNG FPGP L ++NGDTL 
Sbjct: 13  FVAFLLFSSVA--EANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLVVNNGDTLV 72

Query: 65  VTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA 124
           V VIN+A+YN+TIHWHG+RQ RTGWADGPEFVTQCPIRPG SYTYRFT+QGQEGTLWWHA
Sbjct: 73  VKVINRARYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQEGTLWWHA 132

Query: 125 HSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPNI 184
           HSSWLRATVYG+L++ P  G SYPFTKP+R   LL+GEWWDANP+DV+RE+ RTG APN 
Sbjct: 133 HSSWLRATVYGSLLVFPPAGSSYPFTKPHRNVPLLLGEWWDANPVDVLRESIRTGGAPNN 192

Query: 185 SDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGAD 244
           SDAYT NGQPGDLYKCSS+DT +VPI+ GET LLRV+NSALN  LFFTVANHKLTVVGAD
Sbjct: 193 SDAYTINGQPGDLYKCSSQDTTVVPINVGETILLRVINSALNQPLFFTVANHKLTVVGAD 252

Query: 245 ASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYK 304
           ASY KPFTT+V++LGPGQTTDVLI+G+Q P RYY+AARAYQSAQNAPF NTTTTAIL+YK
Sbjct: 253 ASYLKPFTTNVIVLGPGQTTDVLITGDQPPNRYYMAARAYQSAQNAPFGNTTTTAILQYK 312

Query: 305 SAPCPAKKGGA------SIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFV 364
           SAPC    GG+      S +PIMP LPAYNDT TVT FS+SFRS R  EVPTEIDENLFV
Sbjct: 313 SAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNTVTRFSQSFRSLRRAEVPTEIDENLFV 372

Query: 365 TIGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFP 424
           TIGLGLNNCP+NF+S +CQGPNGTRFTASMNNVSF LPSN S+LQA    IPGV T DFP
Sbjct: 373 TIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNVSFALPSNYSLLQAHHHGIPGVFTTDFP 432

Query: 425 ANPPVKFDYTG-NVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDF 484
           A PPVKFDYTG N+SRSL+QP  GTK Y+LKYGSRVQ+VLQDT IVTPENHPIHLHGYDF
Sbjct: 433 AKPPVKFDYTGNNISRSLYQPDRGTKLYKLKYGSRVQIVLQDTGIVTPENHPIHLHGYDF 492

Query: 485 YIVAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHI 544
           YI+AEGFGNFNPK DT KFNL DPPLRNTV VPVNGWAVIRF+ADNPG WIMHCHLD HI
Sbjct: 493 YIIAEGFGNFNPKKDTAKFNLEDPPLRNTVGVPVNGWAVIRFIADNPGVWIMHCHLDAHI 552

Query: 545 TWGLAMVFLVDNGVGQLQSIEAPPADLPIC 568
           +WGLAM FLV+NG G LQ+IE PP DLP+C
Sbjct: 553 SWGLAMAFLVENGNGVLQTIEQPPHDLPVC 580

BLAST of MC04g1844 vs. ExPASy Swiss-Prot
Match: Q9FLB5 (Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 7.7e-268
Identity = 435/570 (76.32%), Postives = 487/570 (85.44%), Query Frame = 0

Query: 1   SLYLFGVLLLFAS--AVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEIS 60
           +++ F +LL F S  +  L   +   H FVIQ TPVKRLCKT NAITVNG FPGPTLE++
Sbjct: 3   TVHTFSILLFFCSLFSASLIIAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVN 62

Query: 61  NGDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG 120
           NGDTL V V N+A+YN+TIHWHG+RQ RTGWADGPEFVTQCPIRPG SYTYRFT+QGQEG
Sbjct: 63  NGDTLEVKVHNRARYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEG 122

Query: 121 TLWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRT 180
           TLWWHAHSSWLRATVYGALII P  G S+PF KP+R+T L++GEWW+ANP+DV+ +ATRT
Sbjct: 123 TLWWHAHSSWLRATVYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRT 182

Query: 181 GAAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKL 240
           GAAPNISDAYT NGQPGDLY CS+K+TV+VPI+SGET+LLRV+N+ALN  LFFTVANHKL
Sbjct: 183 GAAPNISDAYTINGQPGDLYNCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKL 242

Query: 241 TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTT 300
           TVVGADASY KPFTT VLMLGPGQTTDVL++ +Q P RYYIAARAYQSAQNAPFDNTTTT
Sbjct: 243 TVVGADASYLKPFTTKVLMLGPGQTTDVLLTADQPPKRYYIAARAYQSAQNAPFDNTTTT 302

Query: 301 AILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFV 360
           AIL+YK     +K       PIMP LPA+NDT TVT+FSR F+S R V VP  ID+NLF 
Sbjct: 303 AILQYKKTTTTSK-------PIMPVLPAFNDTNTVTSFSRKFKSLRNVVVPKTIDDNLFF 362

Query: 361 TIGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFP 420
           TIGLGL+NCP+ F  S+CQG NGTRFTASMNNVSFVLPSN S+LQA    IPGV T DFP
Sbjct: 363 TIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFP 422

Query: 421 ANPPVKFDYTG-NVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDF 480
           + PPVKFDYTG N+SR+LFQPV GTK Y+LKYGSRVQVVLQDT+IVT ENHPIHLHGYDF
Sbjct: 423 SKPPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDF 482

Query: 481 YIVAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHI 540
           YIV EGFGNFNPK DT KFNLVDPPLRNTVAVPVNGWAVIRFVADNPG W+MHCHLDVHI
Sbjct: 483 YIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHI 542

Query: 541 TWGLAMVFLVDNGVGQLQSIEAPPADLPIC 568
            WGLAM FLVDNGVG+L+++EAPP DLPIC
Sbjct: 543 KWGLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of MC04g1844 vs. ExPASy Swiss-Prot
Match: Q941X2 (Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 7.0e-245
Identity = 393/561 (70.05%), Postives = 452/561 (80.57%), Query Frame = 0

Query: 8   LLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLTVTV 67
           LL  +    LA  + H H+F++Q TPVKRLCKTHN ITVNGQ PGPTLE+  GDT+ + V
Sbjct: 12  LLSCSVLALLAGAEVHHHEFIVQETPVKRLCKTHNVITVNGQLPGPTLEVREGDTVVINV 71

Query: 68  INKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHAHSS 127
           +N A+YNVTIHWHGIRQFRTGWADGPEFVTQCPI+PGGSY YRFT++GQEGTLWWHAHSS
Sbjct: 72  VNHAQYNVTIHWHGIRQFRTGWADGPEFVTQCPIKPGGSYKYRFTIEGQEGTLWWHAHSS 131

Query: 128 WLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPNISDA 187
           WLRATVYGALIIRPRE  +YPF KP RE  L++GEWWDA+PI V+REA RTGAAPNISDA
Sbjct: 132 WLRATVYGALIIRPRENKTYPFEKPAREVPLILGEWWDADPIQVIREAQRTGAAPNISDA 191

Query: 188 YTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGADASY 247
           YT NGQPGDLY CS ++T  VP+  GET LLR +N+ALN  LF ++A HK+TVVG DASY
Sbjct: 192 YTINGQPGDLYNCSKEETTAVPVKPGETALLRFINAALNQELFVSIAQHKMTVVGVDASY 251

Query: 248 TKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYKSAP 307
           TKPFTTSVLM+ PGQTTDVL++ +Q PTRYY+AARAY SAQ   FDNTTTTA++EY    
Sbjct: 252 TKPFTTSVLMIAPGQTTDVLVTMDQAPTRYYLAARAYDSAQGVAFDNTTTTAVIEY-DCG 311

Query: 308 CPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLGLNNC 367
           C A   G SI P  P LPA+NDT T TAF+   RSP  V++P  +DENLF T+G+GL NC
Sbjct: 312 C-ATDFGPSIPPAFPVLPAFNDTNTATAFAAGIRSPHEVKIPGPVDENLFFTVGVGLFNC 371

Query: 368 PRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPVKFDY 427
                  QC GPN TRFTASMNN+SFV P   S+L A    IPGV T DFPA PPV+FDY
Sbjct: 372 E---PGQQCGGPNNTRFTASMNNISFVFPQTTSLLHAHYYGIPGVFTTDFPAYPPVQFDY 431

Query: 428 TG-NVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGFGN 487
           T  NV R L+QPVP TK Y+LK+GS VQ+VLQDTSIV+PENHPIH+HGYDFYI+AEGFGN
Sbjct: 432 TAQNVPRYLWQPVPATKLYKLKFGSVVQIVLQDTSIVSPENHPIHIHGYDFYILAEGFGN 491

Query: 488 FNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAMVFL 547
           F+PK D KKFN VDPP RNTVAVP NGWAVIRFVADNPG W+MHCHLDVHITWGLAM FL
Sbjct: 492 FDPKKDAKKFNYVDPPQRNTVAVPTNGWAVIRFVADNPGVWLMHCHLDVHITWGLAMAFL 551

Query: 548 VDNGVGQLQSIEAPPADLPIC 568
           V++G G+L+++EAPP DLP+C
Sbjct: 552 VEDGYGKLETLEAPPVDLPMC 567

BLAST of MC04g1844 vs. ExPASy Swiss-Prot
Match: Q2RBK2 (Putative laccase-17 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC17 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 6.2e-233
Identity = 383/564 (67.91%), Postives = 449/564 (79.61%), Query Frame = 0

Query: 8   LLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLTVTV 67
           LL  A    LA  +   H+FVI+ T VKRLCK+HN +TVNGQFPGPTLEI+ GD+L + +
Sbjct: 10  LLSLALLCSLAAAKEQYHEFVIRETTVKRLCKSHNIMTVNGQFPGPTLEINEGDSLIINL 69

Query: 68  INKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHAHSS 127
           IN+ +YN+T+HWHG+RQ RTGW+DGPE+VTQCP+RPG SY YRFTV  QEGTLWWHAHSS
Sbjct: 70  INRGRYNMTLHWHGVRQMRTGWSDGPEYVTQCPVRPGQSYRYRFTVAAQEGTLWWHAHSS 129

Query: 128 WLRATVYGALIIRPREGDSYPF-TKPNRE-TTLLMGEWWDANPIDVVREATRTGAAPNIS 187
           WLRATVYGAL+IRPR+G SYPF  +P RE   +L+GEWWD NP+DVVR ATRTGAAPNIS
Sbjct: 130 WLRATVYGALLIRPRDGTSYPFDVQPTRELAPILLGEWWDMNPVDVVRAATRTGAAPNIS 189

Query: 188 DAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGADA 247
           DA T N QPGDLY CSS DT + P+ SGETNLLR +N+ALN  LF ++A H +TVV ADA
Sbjct: 190 DALTVNAQPGDLYSCSSHDTAVFPVTSGETNLLRFINAALNTELFVSLAGHNMTVVAADA 249

Query: 248 SYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYKS 307
           SYTKP+TTS+L+L PGQTTDVL++ +Q P RYY+AARAY SAQ  PFDNTTTTAI +Y +
Sbjct: 250 SYTKPYTTSLLLLAPGQTTDVLVTFDQPPGRYYLAARAYASAQGVPFDNTTTTAIFDYGA 309

Query: 308 APCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLGLN 367
           A        +S    MP+LPAYNDT   TAF+ + R  R  E+P+ +DE+LF T+G+GL 
Sbjct: 310 A-----NNASSAAIAMPTLPAYNDTTAATAFTTNLRGLRKAELPSRVDESLFFTVGVGLF 369

Query: 368 NCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIP-GVITPDFPANPPVK 427
           NC  N  + QC GPNGTRF AS+NNVSFVLPS+ SILQA     P GV T DFPANPPV+
Sbjct: 370 NC-TNATAQQCGGPNGTRFAASINNVSFVLPSSTSILQAHHHGAPGGVFTADFPANPPVQ 429

Query: 428 FDYTG-NVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEG 487
           FDYT  NVSR+L+QPV GTK Y+LKYGS VQVVLQ T+I   ENHPIHLHGYDFYI+AEG
Sbjct: 430 FDYTAQNVSRALWQPVAGTKVYKLKYGSAVQVVLQGTNIFAGENHPIHLHGYDFYILAEG 489

Query: 488 FGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAM 547
            GNF+   DT KFN+ DPP+RNTV VPVNGWAVIRFVADNPG W+MHCHLDVHITWGLAM
Sbjct: 490 LGNFDAGADTGKFNVEDPPMRNTVGVPVNGWAVIRFVADNPGVWLMHCHLDVHITWGLAM 549

Query: 548 VFLVDNGVGQLQSIEAPPADLPIC 568
            FLVD+GVG+LQS+EAPP DLP+C
Sbjct: 550 AFLVDDGVGELQSLEAPPPDLPLC 567

BLAST of MC04g1844 vs. ExPASy Swiss-Prot
Match: Q56YT0 (Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2)

HSP 1 Score: 806.6 bits (2082), Expect = 1.8e-232
Identity = 368/564 (65.25%), Postives = 442/564 (78.37%), Query Frame = 0

Query: 4   LFGVLLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTL 63
           L   + L A    LA  + H HQFVI  TPVKRLC+TH +ITVNGQ+PGPTL + NGD+L
Sbjct: 9   LLSFIALLAYFAFLASAEHHVHQFVITPTPVKRLCRTHQSITVNGQYPGPTLVVRNGDSL 68

Query: 64  TVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH 123
            +TVIN+A+YN++IHWHGIRQ R  WADGPE++TQCPIRPG +YTYRF ++ QEGTLWWH
Sbjct: 69  AITVINRARYNISIHWHGIRQLRNPWADGPEYITQCPIRPGQTYTYRFKIEDQEGTLWWH 128

Query: 124 AHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPN 183
           AHS WLRATVYGALII PR G  YPF+ P R+  +L+GEWWD NP+DV+++A  TGAA N
Sbjct: 129 AHSRWLRATVYGALIIYPRLGSPYPFSMPKRDIPILLGEWWDRNPMDVLKQAQFTGAAAN 188

Query: 184 ISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGA 243
           +SDAYT NGQPGDLY+CS   T+  PI  GET  LRV+N+ +N  LFF+VANH+ TVV  
Sbjct: 189 VSDAYTINGQPGDLYRCSRAGTIRFPIFPGETVQLRVINAGMNQELFFSVANHQFTVVET 248

Query: 244 DASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEY 303
           D++YTKPFTT+V+M+GPGQTT+VL++ NQ P RYY+AARAY SA NAPFDNTTTTAIL+Y
Sbjct: 249 DSAYTKPFTTNVIMIGPGQTTNVLLTANQRPGRYYMAARAYNSA-NAPFDNTTTTAILQY 308

Query: 304 KSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLG 363
            +AP    +G   I P+ P LP +NDTAT TAF+   R  +   VP ++DENLF T+GLG
Sbjct: 309 VNAPTRRGRGRGQIAPVFPVLPGFNDTATATAFTNRLRYWKRAPVPQQVDENLFFTVGLG 368

Query: 364 LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPV 423
           L NC  N  S +CQGPNGTRF ASMNN+SFVLP + S++QA+ Q  PG+ T DFP  PPV
Sbjct: 369 LINC-ANPNSPRCQGPNGTRFAASMNNMSFVLPRSNSVMQAYYQGTPGIFTTDFPPVPPV 428

Query: 424 KFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEG 483
           +FDYTGNVSR L+QP+ GTK Y+LKY S VQ+VLQDTSIVTPENHP+HLHGY FY+V  G
Sbjct: 429 QFDYTGNVSRGLWQPIKGTKAYKLKYKSNVQIVLQDTSIVTPENHPMHLHGYQFYVVGSG 488

Query: 484 FGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAM 543
           FGNFNP+TD  +FNL DPP RNT+  P  GW  IRFVADNPGAW MHCH+D H+ WGLAM
Sbjct: 489 FGNFNPRTDPARFNLFDPPERNTIGTPPGGWVAIRFVADNPGAWFMHCHIDSHLGWGLAM 548

Query: 544 VFLVDNGVGQLQSIEAPPADLPIC 568
           VFLV+NG GQLQS++APP DLP C
Sbjct: 549 VFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of MC04g1844 vs. NCBI nr
Match: XP_038885718.1 (laccase-12-like [Benincasa hispida])

HSP 1 Score: 1107 bits (2864), Expect = 0.0
Identity = 530/568 (93.31%), Postives = 545/568 (95.95%), Query Frame = 0

Query: 1   SLYLFGVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISN 60
           S Y FGVLLL ASA +CLAHPQTHRHQF+IQATPVKRLCKTHNAITVNGQFPGPTLEISN
Sbjct: 12  SHYFFGVLLLLASAALCLAHPQTHRHQFIIQATPVKRLCKTHNAITVNGQFPGPTLEISN 71

Query: 61  GDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGT 120
           GDTL VTVINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGT
Sbjct: 72  GDTLIVTVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGT 131

Query: 121 LWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTG 180
           LWWHAHSSWLR+TVYGALIIRPREGDSYPF KPNRETTLLMGEWWDANPIDVVR+ATRTG
Sbjct: 132 LWWHAHSSWLRSTVYGALIIRPREGDSYPFPKPNRETTLLMGEWWDANPIDVVRQATRTG 191

Query: 181 AAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLT 240
           AAPN+SDAYTFNGQPGDLYKCS+KDT+IVPIDSGETNLLRVVNSALN ALFFTVANHKLT
Sbjct: 192 AAPNVSDAYTFNGQPGDLYKCSNKDTIIVPIDSGETNLLRVVNSALNQALFFTVANHKLT 251

Query: 241 VVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTA 300
           VVGADASYTKPFTTSVLMLGPGQTTDVLISGNQ P+RYYIAARAYQSAQNAPF NTTTTA
Sbjct: 252 VVGADASYTKPFTTSVLMLGPGQTTDVLISGNQAPSRYYIAARAYQSAQNAPFGNTTTTA 311

Query: 301 ILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVT 360
           IL+YKSAPCPAKKG  SI+PIMP LPA+NDTATVTAFSR FRSPRPV VPT+IDENLF T
Sbjct: 312 ILDYKSAPCPAKKGAPSIKPIMPFLPAFNDTATVTAFSRKFRSPRPVPVPTKIDENLFFT 371

Query: 361 IGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA 420
           +GLGLNNCP NFK SQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQ IPGV+T DFPA
Sbjct: 372 VGLGLNNCPPNFKPSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQGIPGVVTSDFPA 431

Query: 421 NPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI 480
           NPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGS VQVVLQDTSIVTPENHPIHLHGYDFYI
Sbjct: 432 NPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSTVQVVLQDTSIVTPENHPIHLHGYDFYI 491

Query: 481 VAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 540
           +AEGFGNFNP TDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW
Sbjct: 492 IAEGFGNFNPTTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 551

Query: 541 GLAMVFLVDNGVGQLQSIEAPPADLPIC 567
           GLAMVFLVDNGVGQLQSIEAPP DLPIC
Sbjct: 552 GLAMVFLVDNGVGQLQSIEAPPPDLPIC 579

BLAST of MC04g1844 vs. NCBI nr
Match: XP_022953900.1 (laccase-12-like [Cucurbita moschata])

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 525/568 (92.43%), Postives = 546/568 (96.13%), Query Frame = 0

Query: 1   SLYLFGVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISN 60
           +L+LFGVLLLF+SA + LAHP THRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLE++N
Sbjct: 7   TLHLFGVLLLFSSAALSLAHPLTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEVNN 66

Query: 61  GDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGT 120
           GDTL VTVINKAKYN TIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTY+FTV+GQEGT
Sbjct: 67  GDTLIVTVINKAKYNATIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYKFTVEGQEGT 126

Query: 121 LWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTG 180
           LWWHAHSSWLRATVYGALIIRPR+GDSYPF KPNRE  LLMGEWWDANPIDVVR+ATRTG
Sbjct: 127 LWWHAHSSWLRATVYGALIIRPRQGDSYPFAKPNREAILLMGEWWDANPIDVVRQATRTG 186

Query: 181 AAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLT 240
           AAPN+SDA+TFNGQPGDLYKCSS+DTVIVPIDSGETNLLRVVNS LN  LFFTVANHKLT
Sbjct: 187 AAPNVSDAFTFNGQPGDLYKCSSRDTVIVPIDSGETNLLRVVNSGLNQPLFFTVANHKLT 246

Query: 241 VVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTA 300
           VVGADASYTKPFTTSVLMLGPGQTTDVLISG+QVP+RYYIAARAYQSAQNAPFDNTTTTA
Sbjct: 247 VVGADASYTKPFTTSVLMLGPGQTTDVLISGDQVPSRYYIAARAYQSAQNAPFDNTTTTA 306

Query: 301 ILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVT 360
           IL YKSAPC AKKGG SI+P+MP LPAYNDTATVTAFSRSFRSPRPV VP EIDENLF T
Sbjct: 307 ILNYKSAPCSAKKGGVSIKPLMPPLPAYNDTATVTAFSRSFRSPRPVPVPKEIDENLFFT 366

Query: 361 IGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA 420
           IGLGLNNCPRNFK SQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA
Sbjct: 367 IGLGLNNCPRNFKPSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA 426

Query: 421 NPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI 480
           NPPVKFDYTGNVS+SLFQP+PGTKGYRLKYGS VQVVLQDTSIVTPENHPIHLHGYDFYI
Sbjct: 427 NPPVKFDYTGNVSQSLFQPIPGTKGYRLKYGSTVQVVLQDTSIVTPENHPIHLHGYDFYI 486

Query: 481 VAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 540
           +AEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW
Sbjct: 487 IAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 546

Query: 541 GLAMVFLVDNGVGQLQSIEAPPADLPIC 567
           GLAMVFLVDNGVGQLQSIE+PP DLPIC
Sbjct: 547 GLAMVFLVDNGVGQLQSIESPPPDLPIC 574

BLAST of MC04g1844 vs. NCBI nr
Match: XP_022993163.1 (laccase-12-like [Cucurbita maxima])

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 528/564 (93.62%), Postives = 542/564 (96.10%), Query Frame = 0

Query: 5   FGVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTL 64
           F VLLLFASA + LAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLE++NGDTL
Sbjct: 12  FAVLLLFASAALSLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEVNNGDTL 71

Query: 65  TVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH 124
            VTVINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH
Sbjct: 72  IVTVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH 131

Query: 125 AHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPN 184
           AHSSWLRATVYGALIIRPR GDS+PF KPNRE  LLMGEWWDANPIDVVR+ATRTGAAPN
Sbjct: 132 AHSSWLRATVYGALIIRPRRGDSFPFPKPNREAILLMGEWWDANPIDVVRQATRTGAAPN 191

Query: 185 ISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGA 244
           +SDAYTFNGQPGD YKCSSKDTV+V IDSGETNLLRVVNSALN ALFFTVANHKLTVVGA
Sbjct: 192 VSDAYTFNGQPGDFYKCSSKDTVVVNIDSGETNLLRVVNSALNQALFFTVANHKLTVVGA 251

Query: 245 DASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEY 304
           DASYTKPFTTSVLMLGPGQTTDVLISGNQVP RYYIAARAYQSAQNAPF NTTTTAIL Y
Sbjct: 252 DASYTKPFTTSVLMLGPGQTTDVLISGNQVPNRYYIAARAYQSAQNAPFGNTTTTAILSY 311

Query: 305 KSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLG 364
           KSAPC AKKG ASI+PIMPSLPAYNDTATVTAFS+ FRSPRPV VPT+IDE+LF TIGLG
Sbjct: 312 KSAPCAAKKGCASIKPIMPSLPAYNDTATVTAFSKKFRSPRPVPVPTKIDESLFFTIGLG 371

Query: 365 LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPV 424
           LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGV+TPDFPANPPV
Sbjct: 372 LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVVTPDFPANPPV 431

Query: 425 KFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEG 484
           KFDYTGNVS+SLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI+AEG
Sbjct: 432 KFDYTGNVSQSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIIAEG 491

Query: 485 FGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAM 544
           FGNFNPKTDTKKFNLVDPPLRNTVAVP NGWAVIRFVADNPGAWIMHCHLDVHITWGLAM
Sbjct: 492 FGNFNPKTDTKKFNLVDPPLRNTVAVPTNGWAVIRFVADNPGAWIMHCHLDVHITWGLAM 551

Query: 545 VFLVDNGVGQLQSIEAPPADLPIC 567
           VFLVDNGVGQLQSIEAPP DLPIC
Sbjct: 552 VFLVDNGVGQLQSIEAPPPDLPIC 575

BLAST of MC04g1844 vs. NCBI nr
Match: KAG7033286.1 (Laccase-12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 524/568 (92.25%), Postives = 546/568 (96.13%), Query Frame = 0

Query: 1   SLYLFGVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISN 60
           +L+LFGVLLLF+SA + LAHP THRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLE++N
Sbjct: 7   TLHLFGVLLLFSSAALSLAHPLTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEVNN 66

Query: 61  GDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGT 120
           GDTL VTVINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTY+FTV+GQEGT
Sbjct: 67  GDTLIVTVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYKFTVEGQEGT 126

Query: 121 LWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTG 180
           LWWHAHSSWLRATVYGALIIRPR+GDSYPF KPNRE  LLMGEWWDANPIDVVR+ATRTG
Sbjct: 127 LWWHAHSSWLRATVYGALIIRPRQGDSYPFAKPNREAILLMGEWWDANPIDVVRQATRTG 186

Query: 181 AAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLT 240
           AAPN+SDA+TFNGQPGDLYKCSS+DTVIVPIDSGETNLLRVVNS LN  LFFTVANHKLT
Sbjct: 187 AAPNVSDAFTFNGQPGDLYKCSSRDTVIVPIDSGETNLLRVVNSGLNQPLFFTVANHKLT 246

Query: 241 VVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTA 300
           VVGADASYTKPFTTSVLMLGPGQTTDVLISG+QVP+RYYIAARAYQSAQNAPFDNTTTTA
Sbjct: 247 VVGADASYTKPFTTSVLMLGPGQTTDVLISGDQVPSRYYIAARAYQSAQNAPFDNTTTTA 306

Query: 301 ILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVT 360
           IL YKSAPC AKKGG SI+P+MP LPAYNDTATVTAFSRSFRSPRPV VP EIDENLF T
Sbjct: 307 ILNYKSAPCSAKKGGVSIKPLMPPLPAYNDTATVTAFSRSFRSPRPVPVPKEIDENLFFT 366

Query: 361 IGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA 420
           IGLGLNNCPRNFK SQCQGPNGTRF ASMNNVSF+LPSNISILQAFQQRIPGVITPDFPA
Sbjct: 367 IGLGLNNCPRNFKPSQCQGPNGTRFAASMNNVSFILPSNISILQAFQQRIPGVITPDFPA 426

Query: 421 NPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI 480
           NPPVKFDYTGNVS+SLFQP+PGTKGYRLKYGS VQVVLQDTSIVTPENHPIHLHGYDFYI
Sbjct: 427 NPPVKFDYTGNVSQSLFQPIPGTKGYRLKYGSTVQVVLQDTSIVTPENHPIHLHGYDFYI 486

Query: 481 VAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 540
           +AEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW
Sbjct: 487 IAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 546

Query: 541 GLAMVFLVDNGVGQLQSIEAPPADLPIC 567
           GLAMVFLVDNGVGQLQSIE+PP DLPIC
Sbjct: 547 GLAMVFLVDNGVGQLQSIESPPPDLPIC 574

BLAST of MC04g1844 vs. NCBI nr
Match: KAG7016026.1 (Laccase-12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1098 bits (2841), Expect = 0.0
Identity = 527/563 (93.61%), Postives = 541/563 (96.09%), Query Frame = 0

Query: 6   GVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLT 65
            VLLLFASA + LAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLE++NGDTL 
Sbjct: 13  AVLLLFASAALSLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEVNNGDTLI 72

Query: 66  VTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA 125
           VTVINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA
Sbjct: 73  VTVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA 132

Query: 126 HSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPNI 185
           HSSWLRATVYGALIIRPR GDS+PF KPNRE  LLMGEWWDANPIDVVR+ATRTGAAPN+
Sbjct: 133 HSSWLRATVYGALIIRPRRGDSFPFPKPNREAILLMGEWWDANPIDVVRQATRTGAAPNV 192

Query: 186 SDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGAD 245
           SDAYTFNGQPGD YKCSSKDTV+V IDSGETNLLRVVNSALN ALFFTVANHKLTVVGAD
Sbjct: 193 SDAYTFNGQPGDFYKCSSKDTVVVNIDSGETNLLRVVNSALNQALFFTVANHKLTVVGAD 252

Query: 246 ASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYK 305
           ASYTKPFTTSVLMLGPGQTTDVLISGNQVP RYYIAARAYQSAQNAPF NTTTTAIL YK
Sbjct: 253 ASYTKPFTTSVLMLGPGQTTDVLISGNQVPNRYYIAARAYQSAQNAPFGNTTTTAILSYK 312

Query: 306 SAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLGL 365
           SAPC AKKG ASI+PIMPSLPAYNDTATVTAFS+ FRSPRPV VPT+IDE+LF TIGLGL
Sbjct: 313 SAPCAAKKGCASIKPIMPSLPAYNDTATVTAFSKKFRSPRPVPVPTKIDESLFFTIGLGL 372

Query: 366 NNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPVK 425
           NNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGV+TPDFPANPPVK
Sbjct: 373 NNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVVTPDFPANPPVK 432

Query: 426 FDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGF 485
           FDYTGNVS+SLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI+AEGF
Sbjct: 433 FDYTGNVSQSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIIAEGF 492

Query: 486 GNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAMV 545
           GNFNPKTDTKKFNLVDPPLRNTVAVP NGWAVIRFVADNPGAWIMHCHLDVHITWGLAMV
Sbjct: 493 GNFNPKTDTKKFNLVDPPLRNTVAVPTNGWAVIRFVADNPGAWIMHCHLDVHITWGLAMV 552

Query: 546 FLVDNGVGQLQSIEAPPADLPIC 567
           FLVDNGVGQLQSIEAPP DLPIC
Sbjct: 553 FLVDNGVGQLQSIEAPPPDLPIC 575

BLAST of MC04g1844 vs. ExPASy TrEMBL
Match: A0A6J1GQY3 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111456301 PE=3 SV=1)

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 525/568 (92.43%), Postives = 546/568 (96.13%), Query Frame = 0

Query: 1   SLYLFGVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISN 60
           +L+LFGVLLLF+SA + LAHP THRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLE++N
Sbjct: 7   TLHLFGVLLLFSSAALSLAHPLTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEVNN 66

Query: 61  GDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGT 120
           GDTL VTVINKAKYN TIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTY+FTV+GQEGT
Sbjct: 67  GDTLIVTVINKAKYNATIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYKFTVEGQEGT 126

Query: 121 LWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTG 180
           LWWHAHSSWLRATVYGALIIRPR+GDSYPF KPNRE  LLMGEWWDANPIDVVR+ATRTG
Sbjct: 127 LWWHAHSSWLRATVYGALIIRPRQGDSYPFAKPNREAILLMGEWWDANPIDVVRQATRTG 186

Query: 181 AAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLT 240
           AAPN+SDA+TFNGQPGDLYKCSS+DTVIVPIDSGETNLLRVVNS LN  LFFTVANHKLT
Sbjct: 187 AAPNVSDAFTFNGQPGDLYKCSSRDTVIVPIDSGETNLLRVVNSGLNQPLFFTVANHKLT 246

Query: 241 VVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTA 300
           VVGADASYTKPFTTSVLMLGPGQTTDVLISG+QVP+RYYIAARAYQSAQNAPFDNTTTTA
Sbjct: 247 VVGADASYTKPFTTSVLMLGPGQTTDVLISGDQVPSRYYIAARAYQSAQNAPFDNTTTTA 306

Query: 301 ILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVT 360
           IL YKSAPC AKKGG SI+P+MP LPAYNDTATVTAFSRSFRSPRPV VP EIDENLF T
Sbjct: 307 ILNYKSAPCSAKKGGVSIKPLMPPLPAYNDTATVTAFSRSFRSPRPVPVPKEIDENLFFT 366

Query: 361 IGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA 420
           IGLGLNNCPRNFK SQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA
Sbjct: 367 IGLGLNNCPRNFKPSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPA 426

Query: 421 NPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI 480
           NPPVKFDYTGNVS+SLFQP+PGTKGYRLKYGS VQVVLQDTSIVTPENHPIHLHGYDFYI
Sbjct: 427 NPPVKFDYTGNVSQSLFQPIPGTKGYRLKYGSTVQVVLQDTSIVTPENHPIHLHGYDFYI 486

Query: 481 VAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 540
           +AEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW
Sbjct: 487 IAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITW 546

Query: 541 GLAMVFLVDNGVGQLQSIEAPPADLPIC 567
           GLAMVFLVDNGVGQLQSIE+PP DLPIC
Sbjct: 547 GLAMVFLVDNGVGQLQSIESPPPDLPIC 574

BLAST of MC04g1844 vs. ExPASy TrEMBL
Match: A0A6J1K1E4 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111489261 PE=3 SV=1)

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 528/564 (93.62%), Postives = 542/564 (96.10%), Query Frame = 0

Query: 5   FGVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTL 64
           F VLLLFASA + LAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLE++NGDTL
Sbjct: 12  FAVLLLFASAALSLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEVNNGDTL 71

Query: 65  TVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH 124
            VTVINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH
Sbjct: 72  IVTVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH 131

Query: 125 AHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPN 184
           AHSSWLRATVYGALIIRPR GDS+PF KPNRE  LLMGEWWDANPIDVVR+ATRTGAAPN
Sbjct: 132 AHSSWLRATVYGALIIRPRRGDSFPFPKPNREAILLMGEWWDANPIDVVRQATRTGAAPN 191

Query: 185 ISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGA 244
           +SDAYTFNGQPGD YKCSSKDTV+V IDSGETNLLRVVNSALN ALFFTVANHKLTVVGA
Sbjct: 192 VSDAYTFNGQPGDFYKCSSKDTVVVNIDSGETNLLRVVNSALNQALFFTVANHKLTVVGA 251

Query: 245 DASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEY 304
           DASYTKPFTTSVLMLGPGQTTDVLISGNQVP RYYIAARAYQSAQNAPF NTTTTAIL Y
Sbjct: 252 DASYTKPFTTSVLMLGPGQTTDVLISGNQVPNRYYIAARAYQSAQNAPFGNTTTTAILSY 311

Query: 305 KSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLG 364
           KSAPC AKKG ASI+PIMPSLPAYNDTATVTAFS+ FRSPRPV VPT+IDE+LF TIGLG
Sbjct: 312 KSAPCAAKKGCASIKPIMPSLPAYNDTATVTAFSKKFRSPRPVPVPTKIDESLFFTIGLG 371

Query: 365 LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPV 424
           LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGV+TPDFPANPPV
Sbjct: 372 LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVVTPDFPANPPV 431

Query: 425 KFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEG 484
           KFDYTGNVS+SLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI+AEG
Sbjct: 432 KFDYTGNVSQSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIIAEG 491

Query: 485 FGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAM 544
           FGNFNPKTDTKKFNLVDPPLRNTVAVP NGWAVIRFVADNPGAWIMHCHLDVHITWGLAM
Sbjct: 492 FGNFNPKTDTKKFNLVDPPLRNTVAVPTNGWAVIRFVADNPGAWIMHCHLDVHITWGLAM 551

Query: 545 VFLVDNGVGQLQSIEAPPADLPIC 567
           VFLVDNGVGQLQSIEAPP DLPIC
Sbjct: 552 VFLVDNGVGQLQSIEAPPPDLPIC 575

BLAST of MC04g1844 vs. ExPASy TrEMBL
Match: A0A6J1FLJ6 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111445331 PE=3 SV=1)

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 526/563 (93.43%), Postives = 541/563 (96.09%), Query Frame = 0

Query: 6   GVLLLFASA-VCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLT 65
            VLLLFASA + LAHPQTHRHQF+IQATPVKRLCKTHNAITVNGQFPGPTLE++NGDTL 
Sbjct: 13  AVLLLFASAALSLAHPQTHRHQFIIQATPVKRLCKTHNAITVNGQFPGPTLEVNNGDTLI 72

Query: 66  VTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA 125
           VTVINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA
Sbjct: 73  VTVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA 132

Query: 126 HSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPNI 185
           HSSWLRATVYGALIIRPR GDS+PF KPNRE  LLMGEWWDANPIDVVR+ATRTGAAPN+
Sbjct: 133 HSSWLRATVYGALIIRPRRGDSFPFPKPNREAILLMGEWWDANPIDVVRQATRTGAAPNV 192

Query: 186 SDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGAD 245
           SDAYTFNGQPGD YKCSSKDTV+V IDSGETNLLRVVNSALN ALFFTVANHKLTVVGAD
Sbjct: 193 SDAYTFNGQPGDFYKCSSKDTVVVNIDSGETNLLRVVNSALNQALFFTVANHKLTVVGAD 252

Query: 246 ASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYK 305
           ASYTKPFTTSVLMLGPGQTTDVLISGNQVP RYYIAARAYQSAQNAPF NTTTTAIL YK
Sbjct: 253 ASYTKPFTTSVLMLGPGQTTDVLISGNQVPNRYYIAARAYQSAQNAPFGNTTTTAILSYK 312

Query: 306 SAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLGL 365
           SAPC AKKG ASI+PIMPSLPAYNDTATVTAFS+ FRSPRPV VPT+IDE+LF TIGLGL
Sbjct: 313 SAPCAAKKGCASIKPIMPSLPAYNDTATVTAFSKKFRSPRPVPVPTKIDESLFFTIGLGL 372

Query: 366 NNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPVK 425
           NNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGV+TPDFPANPPVK
Sbjct: 373 NNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVVTPDFPANPPVK 432

Query: 426 FDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGF 485
           FDYTGNVS+SLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYI+AEGF
Sbjct: 433 FDYTGNVSQSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIIAEGF 492

Query: 486 GNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAMV 545
           GNFNPKTDTKKFNLVDPPLRNTVAVP NGWAVIRFVADNPGAWIMHCHLDVHITWGLAMV
Sbjct: 493 GNFNPKTDTKKFNLVDPPLRNTVAVPTNGWAVIRFVADNPGAWIMHCHLDVHITWGLAMV 552

Query: 546 FLVDNGVGQLQSIEAPPADLPIC 567
           FLVDNGVGQLQSIEAPP DLPIC
Sbjct: 553 FLVDNGVGQLQSIEAPPPDLPIC 575

BLAST of MC04g1844 vs. ExPASy TrEMBL
Match: A0A5A7UNF6 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43052G001500 PE=3 SV=1)

HSP 1 Score: 1097 bits (2837), Expect = 0.0
Identity = 524/569 (92.09%), Postives = 541/569 (95.08%), Query Frame = 0

Query: 1   SLYLFGVLLLFASAVCL--AHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEIS 60
           SLYLF +L+LFASA  L  AHPQTHRHQF+IQATPVKRLCKTHNAITVNGQFPGPTLEI+
Sbjct: 6   SLYLFAILVLFASAAALSLAHPQTHRHQFIIQATPVKRLCKTHNAITVNGQFPGPTLEIN 65

Query: 61  NGDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG 120
           NGDTL V VINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG
Sbjct: 66  NGDTLIVNVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG 125

Query: 121 TLWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRT 180
           TLWWHAHSSWLRATVYGALII P+EGDSYPF KPNRE TLLMGEWWDANPIDV+R+ATRT
Sbjct: 126 TLWWHAHSSWLRATVYGALIIHPKEGDSYPFPKPNREATLLMGEWWDANPIDVLRQATRT 185

Query: 181 GAAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKL 240
           GAAPN+SDAYTFNGQPGDLYKCSSKDT+IVPIDSGETNLLRVVNSALN ALFFTVANHKL
Sbjct: 186 GAAPNVSDAYTFNGQPGDLYKCSSKDTIIVPIDSGETNLLRVVNSALNQALFFTVANHKL 245

Query: 241 TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTT 300
           TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVP RYYIAARAYQSAQNAPF NTTTT
Sbjct: 246 TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPARYYIAARAYQSAQNAPFGNTTTT 305

Query: 301 AILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFV 360
           AIL YKSAPCPAKKG  +I+PIMP LPA+NDTATVTAFSR FRSPRPV VPT+IDENLF 
Sbjct: 306 AILNYKSAPCPAKKGSPTIKPIMPFLPAFNDTATVTAFSRKFRSPRPVPVPTKIDENLFF 365

Query: 361 TIGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFP 420
           T+GLGLNNCPRNFK SQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQ IPGV+T DFP
Sbjct: 366 TVGLGLNNCPRNFKPSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQGIPGVVTSDFP 425

Query: 421 ANPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFY 480
           ANPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGS VQVVLQDTSIVTPENHPIHLHGYDFY
Sbjct: 426 ANPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSTVQVVLQDTSIVTPENHPIHLHGYDFY 485

Query: 481 IVAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHIT 540
           I+AEGFGNF+PK DTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHIT
Sbjct: 486 IIAEGFGNFDPKKDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHIT 545

Query: 541 WGLAMVFLVDNGVGQLQSIEAPPADLPIC 567
           WGLAMVFLVDNGVG LQSIEAPP DLPIC
Sbjct: 546 WGLAMVFLVDNGVGHLQSIEAPPPDLPIC 574

BLAST of MC04g1844 vs. ExPASy TrEMBL
Match: A0A1S3B3L1 (Laccase OS=Cucumis melo OX=3656 GN=LOC103485591 PE=3 SV=1)

HSP 1 Score: 1097 bits (2837), Expect = 0.0
Identity = 524/569 (92.09%), Postives = 541/569 (95.08%), Query Frame = 0

Query: 1   SLYLFGVLLLFASAVCL--AHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEIS 60
           SLYLF +L+LFASA  L  AHPQTHRHQF+IQATPVKRLCKTHNAITVNGQFPGPTLEI+
Sbjct: 6   SLYLFAILVLFASAAALSLAHPQTHRHQFIIQATPVKRLCKTHNAITVNGQFPGPTLEIN 65

Query: 61  NGDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG 120
           NGDTL V VINKAKYNVTIHWHGIRQ RTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG
Sbjct: 66  NGDTLIVNVINKAKYNVTIHWHGIRQLRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG 125

Query: 121 TLWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRT 180
           TLWWHAHSSWLRATVYGALII P+EGDSYPF KPNRE TLLMGEWWDANPIDV+R+ATRT
Sbjct: 126 TLWWHAHSSWLRATVYGALIIHPKEGDSYPFPKPNREATLLMGEWWDANPIDVLRQATRT 185

Query: 181 GAAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKL 240
           GAAPN+SDAYTFNGQPGDLYKCSSKDT+IVPIDSGETNLLRVVNSALN ALFFTVANHKL
Sbjct: 186 GAAPNVSDAYTFNGQPGDLYKCSSKDTIIVPIDSGETNLLRVVNSALNQALFFTVANHKL 245

Query: 241 TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTT 300
           TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVP RYYIAARAYQSAQNAPF NTTTT
Sbjct: 246 TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPARYYIAARAYQSAQNAPFGNTTTT 305

Query: 301 AILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFV 360
           AIL YKSAPCPAKKG  +I+PIMP LPA+NDTATVTAFSR FRSPRPV VPT+IDENLF 
Sbjct: 306 AILNYKSAPCPAKKGSPTIKPIMPFLPAFNDTATVTAFSRKFRSPRPVPVPTKIDENLFF 365

Query: 361 TIGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFP 420
           T+GLGLNNCPRNFK SQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQ IPGV+T DFP
Sbjct: 366 TVGLGLNNCPRNFKPSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQGIPGVVTSDFP 425

Query: 421 ANPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFY 480
           ANPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGS VQVVLQDTSIVTPENHPIHLHGYDFY
Sbjct: 426 ANPPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSTVQVVLQDTSIVTPENHPIHLHGYDFY 485

Query: 481 IVAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHIT 540
           I+AEGFGNF+PK DTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHIT
Sbjct: 486 IIAEGFGNFDPKKDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHIT 545

Query: 541 WGLAMVFLVDNGVGQLQSIEAPPADLPIC 567
           WGLAMVFLVDNGVG LQSIEAPP DLPIC
Sbjct: 546 WGLAMVFLVDNGVGHLQSIEAPPPDLPIC 574

BLAST of MC04g1844 vs. TAIR 10
Match: AT2G40370.1 (laccase 5 )

HSP 1 Score: 934.5 bits (2414), Expect = 4.1e-272
Identity = 438/570 (76.84%), Postives = 487/570 (85.44%), Query Frame = 0

Query: 5   FGVLLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLT 64
           F   LLF+S       + H H+F+IQAT VKRLC+THN+ITVNG FPGP L ++NGDTL 
Sbjct: 13  FVAFLLFSSVA--EANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLVVNNGDTLV 72

Query: 65  VTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHA 124
           V VIN+A+YN+TIHWHG+RQ RTGWADGPEFVTQCPIRPG SYTYRFT+QGQEGTLWWHA
Sbjct: 73  VKVINRARYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQEGTLWWHA 132

Query: 125 HSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPNI 184
           HSSWLRATVYG+L++ P  G SYPFTKP+R   LL+GEWWDANP+DV+RE+ RTG APN 
Sbjct: 133 HSSWLRATVYGSLLVFPPAGSSYPFTKPHRNVPLLLGEWWDANPVDVLRESIRTGGAPNN 192

Query: 185 SDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGAD 244
           SDAYT NGQPGDLYKCSS+DT +VPI+ GET LLRV+NSALN  LFFTVANHKLTVVGAD
Sbjct: 193 SDAYTINGQPGDLYKCSSQDTTVVPINVGETILLRVINSALNQPLFFTVANHKLTVVGAD 252

Query: 245 ASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYK 304
           ASY KPFTT+V++LGPGQTTDVLI+G+Q P RYY+AARAYQSAQNAPF NTTTTAIL+YK
Sbjct: 253 ASYLKPFTTNVIVLGPGQTTDVLITGDQPPNRYYMAARAYQSAQNAPFGNTTTTAILQYK 312

Query: 305 SAPCPAKKGGA------SIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFV 364
           SAPC    GG+      S +PIMP LPAYNDT TVT FS+SFRS R  EVPTEIDENLFV
Sbjct: 313 SAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNTVTRFSQSFRSLRRAEVPTEIDENLFV 372

Query: 365 TIGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFP 424
           TIGLGLNNCP+NF+S +CQGPNGTRFTASMNNVSF LPSN S+LQA    IPGV T DFP
Sbjct: 373 TIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNVSFALPSNYSLLQAHHHGIPGVFTTDFP 432

Query: 425 ANPPVKFDYTG-NVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDF 484
           A PPVKFDYTG N+SRSL+QP  GTK Y+LKYGSRVQ+VLQDT IVTPENHPIHLHGYDF
Sbjct: 433 AKPPVKFDYTGNNISRSLYQPDRGTKLYKLKYGSRVQIVLQDTGIVTPENHPIHLHGYDF 492

Query: 485 YIVAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHI 544
           YI+AEGFGNFNPK DT KFNL DPPLRNTV VPVNGWAVIRF+ADNPG WIMHCHLD HI
Sbjct: 493 YIIAEGFGNFNPKKDTAKFNLEDPPLRNTVGVPVNGWAVIRFIADNPGVWIMHCHLDAHI 552

Query: 545 TWGLAMVFLVDNGVGQLQSIEAPPADLPIC 568
           +WGLAM FLV+NG G LQ+IE PP DLP+C
Sbjct: 553 SWGLAMAFLVENGNGVLQTIEQPPHDLPVC 580

BLAST of MC04g1844 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 924.1 bits (2387), Expect = 5.5e-269
Identity = 435/570 (76.32%), Postives = 487/570 (85.44%), Query Frame = 0

Query: 1   SLYLFGVLLLFAS--AVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEIS 60
           +++ F +LL F S  +  L   +   H FVIQ TPVKRLCKT NAITVNG FPGPTLE++
Sbjct: 3   TVHTFSILLFFCSLFSASLIIAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVN 62

Query: 61  NGDTLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEG 120
           NGDTL V V N+A+YN+TIHWHG+RQ RTGWADGPEFVTQCPIRPG SYTYRFT+QGQEG
Sbjct: 63  NGDTLEVKVHNRARYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEG 122

Query: 121 TLWWHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRT 180
           TLWWHAHSSWLRATVYGALII P  G S+PF KP+R+T L++GEWW+ANP+DV+ +ATRT
Sbjct: 123 TLWWHAHSSWLRATVYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRT 182

Query: 181 GAAPNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKL 240
           GAAPNISDAYT NGQPGDLY CS+K+TV+VPI+SGET+LLRV+N+ALN  LFFTVANHKL
Sbjct: 183 GAAPNISDAYTINGQPGDLYNCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKL 242

Query: 241 TVVGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTT 300
           TVVGADASY KPFTT VLMLGPGQTTDVL++ +Q P RYYIAARAYQSAQNAPFDNTTTT
Sbjct: 243 TVVGADASYLKPFTTKVLMLGPGQTTDVLLTADQPPKRYYIAARAYQSAQNAPFDNTTTT 302

Query: 301 AILEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFV 360
           AIL+YK     +K       PIMP LPA+NDT TVT+FSR F+S R V VP  ID+NLF 
Sbjct: 303 AILQYKKTTTTSK-------PIMPVLPAFNDTNTVTSFSRKFKSLRNVVVPKTIDDNLFF 362

Query: 361 TIGLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFP 420
           TIGLGL+NCP+ F  S+CQG NGTRFTASMNNVSFVLPSN S+LQA    IPGV T DFP
Sbjct: 363 TIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFP 422

Query: 421 ANPPVKFDYTG-NVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDF 480
           + PPVKFDYTG N+SR+LFQPV GTK Y+LKYGSRVQVVLQDT+IVT ENHPIHLHGYDF
Sbjct: 423 SKPPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDF 482

Query: 481 YIVAEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHI 540
           YIV EGFGNFNPK DT KFNLVDPPLRNTVAVPVNGWAVIRFVADNPG W+MHCHLDVHI
Sbjct: 483 YIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHI 542

Query: 541 TWGLAMVFLVDNGVGQLQSIEAPPADLPIC 568
            WGLAM FLVDNGVG+L+++EAPP DLPIC
Sbjct: 543 KWGLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of MC04g1844 vs. TAIR 10
Match: AT2G30210.1 (laccase 3 )

HSP 1 Score: 806.6 bits (2082), Expect = 1.3e-233
Identity = 368/564 (65.25%), Postives = 442/564 (78.37%), Query Frame = 0

Query: 4   LFGVLLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTL 63
           L   + L A    LA  + H HQFVI  TPVKRLC+TH +ITVNGQ+PGPTL + NGD+L
Sbjct: 9   LLSFIALLAYFAFLASAEHHVHQFVITPTPVKRLCRTHQSITVNGQYPGPTLVVRNGDSL 68

Query: 64  TVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWH 123
            +TVIN+A+YN++IHWHGIRQ R  WADGPE++TQCPIRPG +YTYRF ++ QEGTLWWH
Sbjct: 69  AITVINRARYNISIHWHGIRQLRNPWADGPEYITQCPIRPGQTYTYRFKIEDQEGTLWWH 128

Query: 124 AHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWWDANPIDVVREATRTGAAPN 183
           AHS WLRATVYGALII PR G  YPF+ P R+  +L+GEWWD NP+DV+++A  TGAA N
Sbjct: 129 AHSRWLRATVYGALIIYPRLGSPYPFSMPKRDIPILLGEWWDRNPMDVLKQAQFTGAAAN 188

Query: 184 ISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGA 243
           +SDAYT NGQPGDLY+CS   T+  PI  GET  LRV+N+ +N  LFF+VANH+ TVV  
Sbjct: 189 VSDAYTINGQPGDLYRCSRAGTIRFPIFPGETVQLRVINAGMNQELFFSVANHQFTVVET 248

Query: 244 DASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEY 303
           D++YTKPFTT+V+M+GPGQTT+VL++ NQ P RYY+AARAY SA NAPFDNTTTTAIL+Y
Sbjct: 249 DSAYTKPFTTNVIMIGPGQTTNVLLTANQRPGRYYMAARAYNSA-NAPFDNTTTTAILQY 308

Query: 304 KSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLG 363
            +AP    +G   I P+ P LP +NDTAT TAF+   R  +   VP ++DENLF T+GLG
Sbjct: 309 VNAPTRRGRGRGQIAPVFPVLPGFNDTATATAFTNRLRYWKRAPVPQQVDENLFFTVGLG 368

Query: 364 LNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPANPPV 423
           L NC  N  S +CQGPNGTRF ASMNN+SFVLP + S++QA+ Q  PG+ T DFP  PPV
Sbjct: 369 LINC-ANPNSPRCQGPNGTRFAASMNNMSFVLPRSNSVMQAYYQGTPGIFTTDFPPVPPV 428

Query: 424 KFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEG 483
           +FDYTGNVSR L+QP+ GTK Y+LKY S VQ+VLQDTSIVTPENHP+HLHGY FY+V  G
Sbjct: 429 QFDYTGNVSRGLWQPIKGTKAYKLKYKSNVQIVLQDTSIVTPENHPMHLHGYQFYVVGSG 488

Query: 484 FGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAM 543
           FGNFNP+TD  +FNL DPP RNT+  P  GW  IRFVADNPGAW MHCH+D H+ WGLAM
Sbjct: 489 FGNFNPRTDPARFNLFDPPERNTIGTPPGGWVAIRFVADNPGAWFMHCHIDSHLGWGLAM 548

Query: 544 VFLVDNGVGQLQSIEAPPADLPIC 568
           VFLV+NG GQLQS++APP DLP C
Sbjct: 549 VFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of MC04g1844 vs. TAIR 10
Match: AT5G07130.1 (laccase 13 )

HSP 1 Score: 803.1 bits (2073), Expect = 1.4e-232
Identity = 376/563 (66.79%), Postives = 441/563 (78.33%), Query Frame = 0

Query: 8   LLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGDTLTVTV 67
           LLL      L + + H H+FVIQ TPVKRLC+ HN+ITVNGQFPGPTLE+ NGD+L +T 
Sbjct: 9   LLLAIFVASLVNAEVHFHEFVIQETPVKRLCRVHNSITVNGQFPGPTLEVRNGDSLVITA 68

Query: 68  INKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLWWHAHSS 127
           INKA+YN+++HWHGIRQ R  WADGPE++TQCPI+PGGSYTYRFT++ QEGTLWWHAHS 
Sbjct: 69  INKARYNISLHWHGIRQMRNPWADGPEYITQCPIQPGGSYTYRFTMEDQEGTLWWHAHSR 128

Query: 128 WLRATVYGALIIR-PREGDSYPF-TKPNRETTLLMGEWWDANPIDVVREATRTGAAPNIS 187
           WLRATVYGALIIR P     YPF   P RE TLL+GEWWD NP+DV+  A  TGAAPNIS
Sbjct: 129 WLRATVYGALIIRPPLSSPHYPFPVIPKREITLLLGEWWDRNPMDVLNLAQFTGAAPNIS 188

Query: 188 DAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTVVGADA 247
           DA+T NGQPGDLY+CSS++T+   + SGE  LLRV+NSALN  LFF VANHKLTVV ADA
Sbjct: 189 DAFTINGQPGDLYRCSSQETLRFLVGSGEIVLLRVINSALNQELFFGVANHKLTVVAADA 248

Query: 248 SYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAILEYKS 307
           SYTKPF+T+V+MLGPGQTTDVL++ +Q P  YY+AA AY SA NA FDNTTTTAIL+YK 
Sbjct: 249 SYTKPFSTNVIMLGPGQTTDVLLTADQPPAHYYMAAHAYNSA-NAAFDNTTTTAILKYKD 308

Query: 308 APCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTIGLGLN 367
           A C   +  +  R I   LP +NDTAT  AF+   +SP  V+VP EIDENLF T+GLGL 
Sbjct: 309 ASCVTLQAKSQARAIPAQLPGFNDTATAAAFTAQMKSPSKVKVPLEIDENLFFTVGLGLF 368

Query: 368 NCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIP-GVITPDFPANPPVK 427
           NCP    + +CQGPNGTRFTAS+NNVSFV P   SI+QA+ Q  P GV T DFP  PPV 
Sbjct: 369 NCPTP-NTQRCQGPNGTRFTASINNVSFVFPKQNSIMQAYYQGTPTGVFTTDFPPTPPVT 428

Query: 428 FDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGF 487
           FDYTGNVSR L+QP  GTK Y+LK+ S+VQ++LQDTSIVT ENHP+HLHGY+FY+V  G 
Sbjct: 429 FDYTGNVSRGLWQPTRGTKAYKLKFNSQVQIILQDTSIVTTENHPMHLHGYEFYVVGTGV 488

Query: 488 GNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWGLAMV 547
           GNFNP TDT  FNL+DPP RNT+  P  GW  IRFVA+NPGAW+MHCH+D HI WGLAMV
Sbjct: 489 GNFNPNTDTSSFNLIDPPRRNTIGTPPGGWVAIRFVANNPGAWLMHCHIDSHIFWGLAMV 548

Query: 548 FLVDNGVGQLQSIEAPPADLPIC 568
           FLV+NG G LQS+++PP DLP C
Sbjct: 549 FLVENGEGHLQSVQSPPLDLPQC 569

BLAST of MC04g1844 vs. TAIR 10
Match: AT5G09360.1 (laccase 14 )

HSP 1 Score: 590.5 bits (1521), Expect = 1.4e-168
Identity = 283/567 (49.91%), Postives = 365/567 (64.37%), Query Frame = 0

Query: 2   LYLFGVLLLFASAVCLAHPQTHRHQFVIQATPVKRLCKTHNAITVNGQFPGPTLEISNGD 61
           ++   VLL F     +A  + H H F I++    RLC T+  +TVNG+FPGPTL+   GD
Sbjct: 19  VFFLFVLLAFQ----IAEAEIHHHTFKIKSKAYTRLCNTNKILTVNGEFPGPTLKAYRGD 78

Query: 62  TLTVTVINKAKYNVTIHWHGIRQFRTGWADGPEFVTQCPIRPGGSYTYRFTVQGQEGTLW 121
            L V VIN A YN+T+HWHG RQ R  W+DGPE+VTQCPIRPG SY YR  ++ +EGT+W
Sbjct: 79  KLIVNVINNANYNITLHWHGARQIRNPWSDGPEYVTQCPIRPGESYVYRIDLKVEEGTIW 138

Query: 122 WHAHSSWLRATVYGALIIRPREGDSYPFTKPNRETTLLMGEWW-DANPIDVVREATRTGA 181
           WHAHS W RATV+GA I+ P+ G SYPF KP+RE  L++GEWW   N + +  +A +TG 
Sbjct: 139 WHAHSQWARATVHGAFIVYPKRGSSYPFPKPHREIPLILGEWWKKENIMHIPGKANKTGG 198

Query: 182 APNISDAYTFNGQPGDLYKCSSKDTVIVPIDSGETNLLRVVNSALNNALFFTVANHKLTV 241
            P ISD+YT NGQPG LY CS  +T  + +  G   LLR++N+ ++  LFF +ANH LTV
Sbjct: 199 EPAISDSYTINGQPGYLYPCSKPETFKITVVRGRRYLLRIINAVMDEELFFAIANHTLTV 258

Query: 242 VGADASYTKPFTTSVLMLGPGQTTDVLISGNQVPTRYYIAARAYQSAQNAPFDNTTTTAI 301
           V  D  Y K F +  LM+ PGQ+ DVL+  NQ P  Y++AARAY SA  A FD TTTTAI
Sbjct: 259 VAKDGFYLKHFKSDYLMITPGQSMDVLLHANQRPNHYFVAARAYSSAFGAGFDKTTTTAI 318

Query: 302 LEYKSAPCPAKKGGASIRPIMPSLPAYNDTATVTAFSRSFRSPRPVEVPTEIDENLFVTI 361
           L+YK            I+PI+P LP YN T   T F+  FRS RPV VP +I+  L   I
Sbjct: 319 LQYKGDTL------NRIKPILPYLPPYNRTEASTRFTNQFRSQRPVNVPVKINTRLLYAI 378

Query: 362 GLGLNNCPRNFKSSQCQGPNGTRFTASMNNVSFVLPSNISILQAFQQRIPGVITPDFPAN 421
            + L NC        C GP G RF++S+NN+SFV PS + IL+A+ + I GV   DFP N
Sbjct: 379 SVNLMNCS---DDRPCTGPFGKRFSSSINNISFVNPS-VDILRAYYRHIGGVFQEDFPRN 438

Query: 422 PPVKFDYTGNVSRSLFQPVPGTKGYRLKYGSRVQVVLQDTSIVTPENHPIHLHGYDFYIV 481
           PP KF+YTG      F    GTK   L Y S V+++LQ T++     HPIHLHGY+FY+V
Sbjct: 439 PPTKFNYTG--ENLPFPTRFGTKVVVLDYNSSVELILQGTTVWASNIHPIHLHGYNFYVV 498

Query: 482 AEGFGNFNPKTDTKKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGAWIMHCHLDVHITWG 541
             GFGNF+ + D  ++NLVDPP   TV VP NGW  +RFVA+NPG W++HCH++ H TWG
Sbjct: 499 GSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRFVANNPGVWLLHCHIERHATWG 558

Query: 542 LAMVFLVDNGVGQLQSIEAPPADLPIC 568
           +  VF+V +G  +   +  PP DLP C
Sbjct: 559 MNTVFIVKDGPTKSSRMVKPPPDLPSC 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIY85.7e-27176.84Laccase-5 OS=Arabidopsis thaliana OX=3702 GN=LAC5 PE=2 SV=1[more]
Q9FLB57.7e-26876.32Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1[more]
Q941X27.0e-24570.05Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1[more]
Q2RBK26.2e-23367.91Putative laccase-17 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC17 PE=3 SV=1[more]
Q56YT01.8e-23265.25Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_038885718.10.093.31laccase-12-like [Benincasa hispida][more]
XP_022953900.10.092.43laccase-12-like [Cucurbita moschata][more]
XP_022993163.10.093.62laccase-12-like [Cucurbita maxima][more]
KAG7033286.10.092.25Laccase-12, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG7016026.10.093.61Laccase-12, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1GQY30.092.43Laccase OS=Cucurbita moschata OX=3662 GN=LOC111456301 PE=3 SV=1[more]
A0A6J1K1E40.093.62Laccase OS=Cucurbita maxima OX=3661 GN=LOC111489261 PE=3 SV=1[more]
A0A6J1FLJ60.093.43Laccase OS=Cucurbita moschata OX=3662 GN=LOC111445331 PE=3 SV=1[more]
A0A5A7UNF60.092.09Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43052G001500 PE=... [more]
A0A1S3B3L10.092.09Laccase OS=Cucumis melo OX=3656 GN=LOC103485591 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G40370.14.1e-27276.84laccase 5 [more]
AT5G05390.15.5e-26976.32laccase 12 [more]
AT2G30210.11.3e-23365.25laccase 3 [more]
AT5G07130.11.4e-23266.79laccase 13 [more]
AT5G09360.11.4e-16849.91laccase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 30..143
e-value: 6.7E-44
score: 148.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 151..345
e-value: 2.8E-37
score: 130.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 11..141
e-value: 9.7E-39
score: 134.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 365..567
e-value: 2.3E-58
score: 199.3
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 149..344
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 347..567
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 18..168
IPR017761LaccaseTIGRFAMTIGR03389TIGR03389coord: 21..567
e-value: 5.5E-257
score: 851.8
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 155..305
e-value: 2.3E-38
score: 131.8
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 417..549
e-value: 5.3E-41
score: 139.6
NoneNo IPR availablePANTHERPTHR11709:SF355LACCASEcoord: 3..567
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 3..567
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 530..541
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 525..545
IPR034289Laccase, third cupredoxin domainCDDcd13897CuRO_3_LCC_plantcoord: 412..550
e-value: 4.40288E-84
score: 256.032
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 157..303
e-value: 4.60878E-84
score: 256.371
IPR034288Laccase, first cupredoxin domainCDDcd13849CuRO_1_LCC_plantcoord: 25..141
e-value: 2.07264E-74
score: 230.224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g1844.1MC04g1844.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity