MC04g1256 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1256
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Transcription factor GRAS
Location	MC04: 20601417 .. 20602775 (-)
RNA-Seq Expression	MC04g1256
Synteny	MC04g1256

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GACTACTGCTCCCCGGACGCCGATTGGGAGGCCTTCCCCGGCGCCGGCGACTTCCACGACCTAATCGACTCCGTGATGGACGCCGACGTTCCGACGTACGCAGCGGAGGTCGACGGCAGCAGCAACTGCAACTCCGAAGAGGAGGAGTCGACGGAAGCAGAGGATCAGGAAGAGGAGTTGAAAGGGCTACGGTTGTACCACCTGTTAATGGCCGCCGCCGACGCCTTGTACGGCGAGCACAAAAGCCGTGATCTGGCTCACGTGATATTGCTTCGGCTCAACGAATTGGTTTCCCCTTCTCACGGGACTAACCTCGAACGCCTCGCCGCGTATTATACCCAAGCCCTCCAGGGTTTGTTAGAATGCACCGCCGGCGTCGGCAAATCCCACCACCATCTCCACCGCGACGACCACACTCCCGGCGACGTTGTGGCGGCGTTTCAATTGCTGCAGGAGATGTCCCCCTACGTGAAATTCGGCCACTTCACGGCTAATCAGGCGATTCTGGAGGCCGTGGGCGACGACCGTAGGGTCCACATAGTGGACTACGATATAATGGAGGGGATTCAATGGGCGTCGCTGATGCAGGCCTTCGTGTCAAGAAAGGATGGCCCGCCGGCCCCACATCTTAGGATCACGGCTATTTCGAGGGGTGGGAGCACGGCCCGGAGATCAATCGGGACGGTGCAAGAGACCGGGCGGCGATTGGTGGCGTTTGCGGCTTCGATTGGTCAGCCCTTCTCTTTCCATCAGTGTAAATTGGATTCCGACGAGAGTTTCCGGGCCGCCGGGTTGAAATTGGTGAAAGGGGAGGCAGTGGTGGTGAATTGTATGCTGCATCTCCCTCATTTCAGGCACCGTACGCCGGAATCGATAGGGTCGTTTCTGCGGGGCGCCGAGGCGCTGAGTCCGAGAGTGGTGTGTTTGGTGGAGGAGGAAATTGGGCATGGGATTGGCGATTACAAGGTGGAATTCGTGGAGTCGTTGGAGCGTTACTCGGCGATTTACGAGTCGCTGGAGGTAGGGCTGCCGATGAATAACAGGGGAAGGGCGTTGGTGGAGCGGGTGTTCCTGGGGCCACAAATTTGGGGGAGCGTGGGGCTAATGGGGCAGCCGCGGACGGCGGCGGGTTCATGGGGAGAGTGGTTGGGGATGATGGGGATGAAGGCGGCGCCGATTAGCTTCGCGAATCACTGCCAAGCGAGGCTGCTTGTGGGTCTTTTTAACGATGGGTACATAGTGGAGGAATTGTCGAATAATAAGCTGGTTCTTGGATGGAAATCCAAGCGTTTGCTTTCGGCTTCAATTTGGGCATCTTCTTCTTCTCCTTCTTCTTCTTCTTCATCCTCCGATTCCGAA

mRNA sequence

GACTACTGCTCCCCGGACGCCGATTGGGAGGCCTTCCCCGGCGCCGGCGACTTCCACGACCTAATCGACTCCGTGATGGACGCCGACGTTCCGACGTACGCAGCGGAGGTCGACGGCAGCAGCAACTGCAACTCCGAAGAGGAGGAGTCGACGGAAGCAGAGGATCAGGAAGAGGAGTTGAAAGGGCTACGGTTGTACCACCTGTTAATGGCCGCCGCCGACGCCTTGTACGGCGAGCACAAAAGCCGTGATCTGGCTCACGTGATATTGCTTCGGCTCAACGAATTGGTTTCCCCTTCTCACGGGACTAACCTCGAACGCCTCGCCGCGTATTATACCCAAGCCCTCCAGGGTTTGTTAGAATGCACCGCCGGCGTCGGCAAATCCCACCACCATCTCCACCGCGACGACCACACTCCCGGCGACGTTGTGGCGGCGTTTCAATTGCTGCAGGAGATGTCCCCCTACGTGAAATTCGGCCACTTCACGGCTAATCAGGCGATTCTGGAGGCCGTGGGCGACGACCGTAGGGTCCACATAGTGGACTACGATATAATGGAGGGGATTCAATGGGCGTCGCTGATGCAGGCCTTCGTGTCAAGAAAGGATGGCCCGCCGGCCCCACATCTTAGGATCACGGCTATTTCGAGGGGTGGGAGCACGGCCCGGAGATCAATCGGGACGGTGCAAGAGACCGGGCGGCGATTGGTGGCGTTTGCGGCTTCGATTGGTCAGCCCTTCTCTTTCCATCAGTGTAAATTGGATTCCGACGAGAGTTTCCGGGCCGCCGGGTTGAAATTGGTGAAAGGGGAGGCAGTGGTGGTGAATTGTATGCTGCATCTCCCTCATTTCAGGCACCGTACGCCGGAATCGATAGGGTCGTTTCTGCGGGGCGCCGAGGCGCTGAGTCCGAGAGTGGTGTGTTTGGTGGAGGAGGAAATTGGGCATGGGATTGGCGATTACAAGGTGGAATTCGTGGAGTCGTTGGAGCGTTACTCGGCGATTTACGAGTCGCTGGAGGTAGGGCTGCCGATGAATAACAGGGGAAGGGCGTTGGTGGAGCGGGTGTTCCTGGGGCCACAAATTTGGGGGAGCGTGGGGCTAATGGGGCAGCCGCGGACGGCGGCGGGTTCATGGGGAGAGTGGTTGGGGATGATGGGGATGAAGGCGGCGCCGATTAGCTTCGCGAATCACTGCCAAGCGAGGCTGCTTGTGGGTCTTTTTAACGATGGGTACATAGTGGAGGAATTGTCGAATAATAAGCTGGTTCTTGGATGGAAATCCAAGCGTTTGCTTTCGGCTTCAATTTGGGCATCTTCTTCTTCTCCTTCTTCTTCTTCTTCATCCTCCGATTCCGAA

Coding sequence (CDS)

GACTACTGCTCCCCGGACGCCGATTGGGAGGCCTTCCCCGGCGCCGGCGACTTCCACGACCTAATCGACTCCGTGATGGACGCCGACGTTCCGACGTACGCAGCGGAGGTCGACGGCAGCAGCAACTGCAACTCCGAAGAGGAGGAGTCGACGGAAGCAGAGGATCAGGAAGAGGAGTTGAAAGGGCTACGGTTGTACCACCTGTTAATGGCCGCCGCCGACGCCTTGTACGGCGAGCACAAAAGCCGTGATCTGGCTCACGTGATATTGCTTCGGCTCAACGAATTGGTTTCCCCTTCTCACGGGACTAACCTCGAACGCCTCGCCGCGTATTATACCCAAGCCCTCCAGGGTTTGTTAGAATGCACCGCCGGCGTCGGCAAATCCCACCACCATCTCCACCGCGACGACCACACTCCCGGCGACGTTGTGGCGGCGTTTCAATTGCTGCAGGAGATGTCCCCCTACGTGAAATTCGGCCACTTCACGGCTAATCAGGCGATTCTGGAGGCCGTGGGCGACGACCGTAGGGTCCACATAGTGGACTACGATATAATGGAGGGGATTCAATGGGCGTCGCTGATGCAGGCCTTCGTGTCAAGAAAGGATGGCCCGCCGGCCCCACATCTTAGGATCACGGCTATTTCGAGGGGTGGGAGCACGGCCCGGAGATCAATCGGGACGGTGCAAGAGACCGGGCGGCGATTGGTGGCGTTTGCGGCTTCGATTGGTCAGCCCTTCTCTTTCCATCAGTGTAAATTGGATTCCGACGAGAGTTTCCGGGCCGCCGGGTTGAAATTGGTGAAAGGGGAGGCAGTGGTGGTGAATTGTATGCTGCATCTCCCTCATTTCAGGCACCGTACGCCGGAATCGATAGGGTCGTTTCTGCGGGGCGCCGAGGCGCTGAGTCCGAGAGTGGTGTGTTTGGTGGAGGAGGAAATTGGGCATGGGATTGGCGATTACAAGGTGGAATTCGTGGAGTCGTTGGAGCGTTACTCGGCGATTTACGAGTCGCTGGAGGTAGGGCTGCCGATGAATAACAGGGGAAGGGCGTTGGTGGAGCGGGTGTTCCTGGGGCCACAAATTTGGGGGAGCGTGGGGCTAATGGGGCAGCCGCGGACGGCGGCGGGTTCATGGGGAGAGTGGTTGGGGATGATGGGGATGAAGGCGGCGCCGATTAGCTTCGCGAATCACTGCCAAGCGAGGCTGCTTGTGGGTCTTTTTAACGATGGGTACATAGTGGAGGAATTGTCGAATAATAAGCTGGTTCTTGGATGGAAATCCAAGCGTTTGCTTTCGGCTTCAATTTGGGCATCTTCTTCTTCTCCTTCTTCTTCTTCTTCATCCTCCGATTCCGAA

Protein sequence

DYCSPDADWEAFPGAGDFHDLIDSVMDADVPTYAAEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVGKSHHHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGLMGQPRTAAGSWGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNKLVLGWKSKRLLSASIWASSSSPSSSSSSSDSE

Homology

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BLAST of MC04g1256 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.4e-139
Identity = 269/465 (57.85%), Postives = 331/465 (71.18%), Query Frame = 0


Query: 1   DYCSPDADWEAFPG-AGDFHDLIDSVMDADVPTYAAEVDGSSNCNSEEEESTEA------ 60
           ++ SP  +W+AF G   DFH LIDS++D D  T  A  D +++  SEEEE  EA      
Sbjct: 35  NHWSPVVNWDAFTGNQDDFHHLIDSMID-DNNTGPAFSDHTASTTSEEEEEEEATTTTMT 94

Query: 61  ----------EDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGT 120
                     E  +++ KGLRL HLLMA A+AL G +K+R+LA VIL+RL ELVS + GT
Sbjct: 95  TTTTTTTTTPEAADDDFKGLRLVHLLMAGAEALTGANKNRELARVILVRLKELVSHTDGT 154

Query: 121 NLERLAAYYTQALQGLLECTAGVGKS---HHHLHRDDHTP-GDVVAAFQLLQEMSPYVKF 180
           N+ERLAAY+T+ALQGLLE   G   S   HH +    H P  D +AAFQLLQ+MSPYVKF
Sbjct: 155 NMERLAAYFTEALQGLLEGAGGAYNSSSKHHVIGGPHHEPQNDALAAFQLLQDMSPYVKF 214

Query: 181 GHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGG 240
           GHFTANQAI+EAV  +RRVHIVDYDIMEG+QWASLMQA  S  +G   PHLRITA+SR G
Sbjct: 215 GHFTANQAIVEAVAHERRVHIVDYDIMEGVQWASLMQALASNPNG---PHLRITALSRSG 274

Query: 241 STARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGEAVVVNCML 300
              RRS+ TVQETGRRL AFA S+GQPFSFH  +L+SDE+FR AGLKLV+GEA+V NCML
Sbjct: 275 -VGRRSMATVQETGRRLTAFATSLGQPFSFHHSRLESDETFRPAGLKLVRGEALVFNCML 334

Query: 301 HLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESL 360
           +LPH  +R+P S+ SFL  A+AL PR+V +VEEE+G  +G +   F++SL  +SA+++SL
Sbjct: 335 NLPHLTYRSPNSVASFLTAAKALRPRLVTVVEEEVGSALGGFVERFMDSLHHFSAVFDSL 394

Query: 361 EVGLPMNNRGRALVERVFLGPQIWGSVGLM---GQPRTAAGSWGEWLGMMGMKAAPISFA 420
           E G PM  R RALVERVFLGP+I GS+  +   G      GSW EWL   G     +S A
Sbjct: 395 EAGFPMQGRARALVERVFLGPRIVGSLARIYRTGGGGEERGSWREWLRAAGFSGVAVSSA 454

Query: 421 NHCQARLLVGLFNDGYIVEELSNNKLVLGWKSKRLLSASIWASSS 442
           NHCQ+ LL+GLFNDGY VEEL +NKLVL WK++RLLSAS+W  SS
Sbjct: 455 NHCQSNLLLGLFNDGYRVEELGSNKLVLHWKTRRLLSASLWTCSS 494

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BLAST of MC04g1256 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.4e-139
Identity = 262/477 (54.93%), Postives = 339/477 (71.07%), Query Frame = 0


Query: 1   DYCSPDADWEAFPGA-GDFHDLIDSVMDADVPTYAAEVDGSSNCNSEEEESTEAEDQE-- 60
           ++ SP  +W+ F GA  DFH L+D++++ D  T   ++  S    +    +T+ E++E  
Sbjct: 35  NHWSPIVNWDTFTGAPDDFHHLMDTIIE-DRTTVLEQLSPSITTTTTTTTTTDEEEEEME 94

Query: 61  ----------------EELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSP-S 120
                           ++ KGL+L HLLMA A+AL G  K+RDLA VIL+RL ELVS  +
Sbjct: 95  TTTTTTTTAIKTHEVGDDSKGLKLVHLLMAGAEALTGSTKNRDLARVILIRLKELVSQHA 154

Query: 121 HGTNLERLAAYYTQALQGLLECTAGVGKSHHHLHRDDH---------TPGDVVAAFQLLQ 180
           +G+N+ERLAA++T+AL GLLE   G   +HHH + + H            D +AAFQLLQ
Sbjct: 155 NGSNMERLAAHFTEALHGLLEGAGGAHNNHHHHNNNKHYLTTNGPHDNQNDTLAAFQLLQ 214

Query: 181 EMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLR 240
           +MSPYVKFGHFTANQAI+EAV  +RRVH++DYDIMEG+QWASL+Q+  S  +G   PHLR
Sbjct: 215 DMSPYVKFGHFTANQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNG---PHLR 274

Query: 241 ITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGE 300
           ITA+SR G T RRSI TVQETGRRL +FAAS+GQPFSFH C+LDSDE+FR + LKLV+GE
Sbjct: 275 ITALSRTG-TGRRSIATVQETGRRLTSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGE 334

Query: 301 AVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLER 360
           A+V NCML+LPH  +R PES+ SFL GA+ L+P++V LVEEE+G  IG +   F++SL  
Sbjct: 335 ALVFNCMLNLPHLSYRAPESVASFLNGAKTLNPKLVTLVEEEVGSVIGGFVERFMDSLHH 394

Query: 361 YSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGLMGQP--RTAAGSWGEWLGMMGMK 420
           YSA+++SLE G PM NR R LVERVF GP+I GS+G + +        SWGEWLG +G +
Sbjct: 395 YSAVFDSLEAGFPMQNRARTLVERVFFGPRIAGSLGRIYRTGGEEERRSWGEWLGEVGFR 454

Query: 421 AAPISFANHCQARLLVGLFNDGYIVEE--LSNNKLVLGWKSKRLLSASIWASSSSPS 445
             P+SFANHCQA+LL+GLFNDGY VEE  + +NKLVL WKS+RLLSAS+W  SSS S
Sbjct: 455 GVPVSFANHCQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKSRRLLSASLWTCSSSDS 506

[Close]

BLAST of MC04g1256 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 5.5e-115
Identity = 255/507 (50.30%), Postives = 308/507 (60.75%), Query Frame = 0


Query: 4   SPDADWEAF-----PGAGDFHDLIDSVMDADVPTYAAEVDGSSNC--------------N 63
           SP ADW  F      G  D H LI+S++  D      + DG +                N
Sbjct: 43  SPVADWANFCCSDDDGGHDLHGLIESML-CDDTLVGVDDDGQAGLHHADMFRDDLYCYGN 102

Query: 64  SEEEESTEAEDQEEEL----------KGLRLYHLLMAAADALYGEHKSRDLAHVILLRLN 123
                ST   +    +          KGLRL HLLMAAA+AL G HKSR+LA VIL+RL 
Sbjct: 103 GSNPSSTTTTNPGSPVFDDPTQGCPEKGLRLLHLLMAAAEALSGPHKSRELARVILVRLK 162

Query: 124 ELVS-----PSHGTNLERLAAYYTQALQGLLECTAGVG---------KSHHHLHRDDHTP 183
           E+VS      +  +N+ERLAA++T ALQGLL+ +  VG          SHHH        
Sbjct: 163 EMVSHTASANAAASNMERLAAHFTDALQGLLDGSHPVGGSGRQAAAAASHHH-------A 222

Query: 184 GDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVS 243
           GDV+ AFQ+LQ+MSPY+KFGHFTANQAILEAV  DRRVHIVDYDI EGIQWASLMQA  S
Sbjct: 223 GDVLTAFQMLQDMSPYMKFGHFTANQAILEAVSGDRRVHIVDYDIAEGIQWASLMQAMTS 282

Query: 244 RKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESF 303
           R DG PAPHLRITA+SR G    R+   VQE GRRL AFAASIGQPFSF QC+LDSDE F
Sbjct: 283 RADGVPAPHLRITAVSRSGGGGARA---VQEAGRRLSAFAASIGQPFSFGQCRLDSDERF 342

Query: 304 RAAGLKLVKGEAVVVNCMLHLPHFR---HRTPESIGSFLRGAEALSPRVVCLVEEE---- 363
           R A +++VKGEA+V NC+LH         R   S+ SFL G  AL  ++V +VEEE    
Sbjct: 343 RPATVRMVKGEALVANCVLHQAAATTTIRRPTGSVASFLSGMAALGAKLVTVVEEEGEAE 402

Query: 364 ---IGHGIGD-----YKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGS 423
               G   GD     +  +F+E L RYSA+++SLE G P  +R R LVERV L P I G+
Sbjct: 403 KDDDGDSAGDAAAGGFVRQFMEELHRYSAVWDSLEAGFPTQSRVRGLVERVILAPNIAGA 462

Query: 424 V-----GLMGQPRTAAGSWGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNN 448
           V     G+ G+ R     WG+W+   G  A P+S  NH QARLL+GLFNDGY VEE   N
Sbjct: 463 VSRAYRGVDGEGRC---GWGQWMRGSGFTAVPLSCFNHSQARLLLGLFNDGYTVEETGPN 522

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BLAST of MC04g1256 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.0e-113
Identity = 233/452 (51.55%), Postives = 296/452 (65.49%), Query Frame = 0


Query: 6   DADWEAFPGAGDFHDLIDSVMDADVPTYAAEV-------DGSSNCNSEEEESTEA----- 65
           D DW+      DF D+I+S+M  +      E        D    CNS     + A     
Sbjct: 37  DIDWDC-----DFRDVIESIMGDEGAMMEPESEAVPMLHDQEGLCNSASTGLSVADGVSF 96

Query: 66  -EDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYY 125
            E + +E KGLRL HLL+AAADA  G +KSR+L  VIL RL +LVSP   TN+ERLAA++
Sbjct: 97  GEPKTDESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHF 156

Query: 126 TQALQGLLECTAGVGKSHHHLHRDD-HTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEA 185
           T  L  LLE  + +       HRDD +   DV++AF+LLQ MSPYV FG+ TA QAILEA
Sbjct: 157 TNGLSKLLERDSVLCPQQ---HRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEA 216

Query: 186 VGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQE 245
           V  +RR+HIVDYDI EG+QWASLMQA VSR  GP A HLRITA+SR  +  ++S+  VQE
Sbjct: 217 VKYERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSR-ATNGKKSVAAVQE 276

Query: 246 TGRRLVAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPES 305
           TGRRL AFA SIGQPFS+  CKLD++ +F  + LKLV+GEAVV+NCMLHLP F H+TP S
Sbjct: 277 TGRRLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSS 336

Query: 306 IGSFLRGAEALSPRVVCLVEEEIG-HGIGDYKVEFVESLERYSAIYESLEVGLPMNNRGR 365
           + SFL  A+ L+P++V LV EE+G  G   +   F++ L ++SAI++SLE GL + N  R
Sbjct: 337 VISFLSEAKTLNPKLVTLVHEEVGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPAR 396

Query: 366 ALVERVFLGPQI--W-GSVGLMGQPRTAAGSWGEWLGMMGMKAAPISFANHCQARLLVGL 425
             VERVF+GP +  W   +        +  SW +WL   G K   +SF N CQA+LL+ L
Sbjct: 397 GFVERVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSL 456

Query: 426 FNDGYIVEELSNNKLVLGWKSKRLLSASIWAS 440
           FNDG+ VEEL  N LVLGWKS+RL+SAS WAS
Sbjct: 457 FNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

[Close]

BLAST of MC04g1256 vs. ExPASy Swiss-Prot
Match: Q2RB59 (Protein SCARECROW 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SCR1 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.3e-51
Identity = 143/414 (34.54%), Postives = 218/414 (52.66%), Query Frame = 0


Query: 31  PTYAAEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVIL 90
           P  A E   ++    E +E    + ++EE  GL L  LL+  A+++  +  + D AH  L
Sbjct: 251 PPTAEETAAAAAAAKERKEEQRRKQRDEE--GLHLLTLLLQCAESVNAD--NLDEAHRAL 310

Query: 91  LRLNELVSPSHGTNLERLAAYYTQALQGLL--ECTAGVGKSHHHLHRDDHTPGDVVAAFQ 150
           L + EL +P  GT+ +R+AAY+ +A+   L   C        +         G V AAFQ
Sbjct: 311 LEIAELATP-FGTSTQRVAAYFAEAMSARLVSSCLGLYAPLPNPSPAAARLHGRVAAAFQ 370

Query: 151 LLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAP 210
           +   +SP+VKF HFTANQAI EA   + RVHI+D DIM+G+QW  L     SR  GP  P
Sbjct: 371 VFNGISPFVKFSHFTANQAIQEAFEREERVHIIDLDIMQGLQWPGLFHILASRPGGP--P 430

Query: 211 HLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKL-DSDESFRAAGLKL 270
            +R+T +         S+  ++ TG+RL  FA ++G PF F  C + D   +     L +
Sbjct: 431 RVRLTGLG-------ASMEALEATGKRLSDFADTLGLPFEF--CPVADKAGNLDPEKLGV 490

Query: 271 VKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVE 330
            + EAV V+    L H  +    S  + L   + L+P+VV +VE+++ H  G +   FVE
Sbjct: 491 TRREAVAVH---WLRHSLYDVTGSDSNTLWLIQRLAPKVVTMVEQDLSHS-GSFLARFVE 550

Query: 331 SLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGLMGQPRTA---AGSWGEWLG 390
           ++  YSA+++SL+     ++  R +VE+  L  +I   + + G  RT     GSW E L 
Sbjct: 551 AIHYYSALFDSLDASYSEDSPERHVVEQQLLSREIRNVLAVGGPARTGDVKFGSWREKLA 610

Query: 391 MMGMKAAPISFANHCQARLLVGLF-NDGYIVEELSNNKLVLGWKSKRLLSASIW 438
             G + + ++ +   QA LL+G+F +DGY + E  N  L LGWK   LL+AS W
Sbjct: 611 QSGFRVSSLAGSAAAQAVLLLGMFPSDGYTLIE-ENGALKLGWKDLCLLTASAW 643

[Close]

BLAST of MC04g1256 vs. NCBI nr
Match: XP_022993952.1 (nodulation-signaling pathway 2 protein [Cucurbita maxima])

HSP 1 Score: 619 bits (1596), Expect = 2.02e-218
Identity = 332/452 (73.45%), Postives = 374/452 (82.74%), Query Frame = 0


Query: 17  DFHDLIDSVMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    EVDG +NCNS    + + E++EEE K LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEVDGGTNCNSPSNPAEDREEEEEEFKSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G++KSR+LA VIL+RLNELVS SHGTNLERL AYY QA Q LL+C A   GV    
Sbjct: 87  DAMFGDNKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQ+MSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQAFVSRKD PPAPHLRIT ISRG S+ RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQAFVSRKDSPPAPHLRITVISRGPSS-RRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKLDSDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 LMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
            +GQP+ AA +      WGE L  MG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           LVLGWKSKRLLS SIWASSSS SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSSS-SSSSSSSDSE 476

[Close]

BLAST of MC04g1256 vs. NCBI nr
Match: XP_023551167.1 (nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615 bits (1586), Expect = 6.68e-217
Identity = 332/452 (73.45%), Postives = 371/452 (82.08%), Query Frame = 0


Query: 17  DFHDLIDSVMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    EVDG +NCNS    + + E++EEE K LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEVDGGTNCNSPSNPAEDREEEEEEFKSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G+HKSRDLA VIL+RLNELVS SHGTNLERL AYY Q+ Q LL+  A   G     
Sbjct: 87  DAMFGDHKSRDLAQVILIRLNELVSTSHGTNLERLTAYYAQSFQDLLDYAAVSGGGSNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S+ RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPSS-RRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKLDSDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIDSFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLK 386

Query: 377 LMGQPRTAAG------SWGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
            +GQP+ AA       SWGE L  MG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCSWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           LVLGWKSKRLLS SIWASSS  SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS-LSSSSSSSDSE 476

[Close]

BLAST of MC04g1256 vs. NCBI nr
Match: KAG6578977.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 614 bits (1584), Expect = 1.34e-216
Identity = 330/452 (73.01%), Postives = 371/452 (82.08%), Query Frame = 0


Query: 17  DFHDLIDSVMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVG---KSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLERL AYY QA Q LL+C A  G      
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S+ RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPSS-RRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKLDSDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 LMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
            +GQP+ AA +      WGE L  MG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           LVLGWKSKRLLS SIWASSS  SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS-LSSSSSSSDSE 476

[Close]

BLAST of MC04g1256 vs. NCBI nr
Match: KAG7016501.1 (Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 613 bits (1582), Expect = 2.71e-216
Identity = 329/452 (72.79%), Postives = 371/452 (82.08%), Query Frame = 0


Query: 17  DFHDLIDSVMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVG---KSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLER+ AYY QA Q LL+C A  G      
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S+ RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPSS-RRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKLDSDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 LMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
            +GQP+ AA +      WGE L  MG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           LVLGWKSKRLLS SIWASSS  SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS-LSSSSSSSDSE 476

[Close]

BLAST of MC04g1256 vs. NCBI nr
Match: XP_022938560.1 (nodulation-signaling pathway 2 protein [Cucurbita moschata])

HSP 1 Score: 610 bits (1574), Expect = 4.45e-215
Identity = 328/452 (72.57%), Postives = 370/452 (81.86%), Query Frame = 0


Query: 17  DFHDLIDSVMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVG---KSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLER+ AYY QA Q LL+C A  G      
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S+ RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPSS-RRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKLDSDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 LMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
            + QP+ AA +      WGE L  MG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIEQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           LVLGWKSKRLLS SIWASSS  SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS-LSSSSSSSDSE 476

[Close]

BLAST of MC04g1256 vs. ExPASy TrEMBL
Match: A0A6J1JUC4 (nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC111489805 PE=3 SV=1)

HSP 1 Score: 619 bits (1596), Expect = 9.76e-219
Identity = 332/452 (73.45%), Postives = 374/452 (82.74%), Query Frame = 0


Query: 17  DFHDLIDSVMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    EVDG +NCNS    + + E++EEE K LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEVDGGTNCNSPSNPAEDREEEEEEFKSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G++KSR+LA VIL+RLNELVS SHGTNLERL AYY QA Q LL+C A   GV    
Sbjct: 87  DAMFGDNKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQ+MSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQAFVSRKD PPAPHLRIT ISRG S+ RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQAFVSRKDSPPAPHLRITVISRGPSS-RRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKLDSDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 LMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
            +GQP+ AA +      WGE L  MG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           LVLGWKSKRLLS SIWASSSS SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSSS-SSSSSSSDSE 476

[Close]

BLAST of MC04g1256 vs. ExPASy TrEMBL
Match: A0A6J1FK48 (nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC111444761 PE=3 SV=1)

HSP 1 Score: 610 bits (1574), Expect = 2.16e-215
Identity = 328/452 (72.57%), Postives = 370/452 (81.86%), Query Frame = 0


Query: 17  DFHDLIDSVMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVG---KSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLER+ AYY QA Q LL+C A  G      
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S+ RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPSS-RRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKLDSDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 LMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
            + QP+ AA +      WGE L  MG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIEQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           LVLGWKSKRLLS SIWASSS  SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS-LSSSSSSSDSE 476

[Close]

BLAST of MC04g1256 vs. ExPASy TrEMBL
Match: A0A5A7V767 (Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00580 PE=3 SV=1)

HSP 1 Score: 600 bits (1546), Expect = 4.64e-211
Identity = 328/466 (70.39%), Postives = 366/466 (78.54%), Query Frame = 0


Query: 2   YCSPDADWEAFPGA-GDFHDLIDSVMDADVPTYAAEVDGSSNCNSEEEESTEAEDQEEEL 61
           Y SP  DWE F G   DF D+ DS +  + P +                 TE  D+E+E 
Sbjct: 36  YSSPIVDWETFSGTHNDFLDVFDSTICIENPPFL----------------TEDRDEEDEF 95

Query: 62  KGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLL 121
           KGLRLYHLL+AAADA++G+H+S DLAHVIL+RLNELVSPSHGTNLERL AYY QA Q LL
Sbjct: 96  KGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLL 155

Query: 122 ECTAGVGKSHHHLH----RDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDR 181
           +      K+HHH H    RDDHTP DV+AAFQLLQEMSPYVKFGHFTANQAILEAV +DR
Sbjct: 156 DSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDR 215

Query: 182 RVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRL 241
           RVHIVDYDIMEGIQWASLMQAFVS    P APHLRITAISRG +  RRSIGTVQETGRRL
Sbjct: 216 RVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGAN-GRRSIGTVQETGRRL 275

Query: 242 VAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFL 301
           VAFAASIGQPFSFHQCKLDSDESFR +GLKLVKGEA+VVNCMLHLPHF +R+PESI SFL
Sbjct: 276 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFL 335

Query: 302 RGAEALSPRVVCLVEEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALV 361
            GA++LSPR+V LVEEEIGHG    GDYKV+F++SLERYSAIY+SLE  +PM NR RALV
Sbjct: 336 SGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALV 395

Query: 362 ERVFLGPQIWGSVGLMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGL 421
           ERVFLGP+I  ++  +GQ R +  +      WGE L  MG+K   ISFANHCQARLL+GL
Sbjct: 396 ERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGL 455

Query: 422 FNDGYIVEELSNNKLVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           FNDGY VEEL NNKLVLGWKSKRLLS SIW SS+S SSSSS SDSE
Sbjct: 456 FNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

[Close]

BLAST of MC04g1256 vs. ExPASy TrEMBL
Match: A0A1S3BE54 (nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 PE=3 SV=1)

HSP 1 Score: 600 bits (1546), Expect = 4.64e-211
Identity = 328/466 (70.39%), Postives = 366/466 (78.54%), Query Frame = 0


Query: 2   YCSPDADWEAFPGA-GDFHDLIDSVMDADVPTYAAEVDGSSNCNSEEEESTEAEDQEEEL 61
           Y SP  DWE F G   DF D+ DS +  + P +                 TE  D+E+E 
Sbjct: 36  YSSPIVDWETFSGTHNDFLDVFDSTICIENPPFL----------------TEDRDEEDEF 95

Query: 62  KGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLL 121
           KGLRLYHLL+AAADA++G+H+S DLAHVIL+RLNELVSPSHGTNLERL AYY QA Q LL
Sbjct: 96  KGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLL 155

Query: 122 ECTAGVGKSHHHLH----RDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDR 181
           +      K+HHH H    RDDHTP DV+AAFQLLQEMSPYVKFGHFTANQAILEAV +DR
Sbjct: 156 DSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDR 215

Query: 182 RVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRL 241
           RVHIVDYDIMEGIQWASLMQAFVS    P APHLRITAISRG +  RRSIGTVQETGRRL
Sbjct: 216 RVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGAN-GRRSIGTVQETGRRL 275

Query: 242 VAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFL 301
           VAFAASIGQPFSFHQCKLDSDESFR +GLKLVKGEA+VVNCMLHLPHF +R+PESI SFL
Sbjct: 276 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFL 335

Query: 302 RGAEALSPRVVCLVEEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALV 361
            GA++LSPR+V LVEEEIGHG    GDYKV+F++SLERYSAIY+SLE  +PM NR RALV
Sbjct: 336 SGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALV 395

Query: 362 ERVFLGPQIWGSVGLMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGL 421
           ERVFLGP+I  ++  +GQ R +  +      WGE L  MG+K   ISFANHCQARLL+GL
Sbjct: 396 ERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGL 455

Query: 422 FNDGYIVEELSNNKLVLGWKSKRLLSASIWASSSSPSSSSSSSDSE 453
           FNDGY VEEL NNKLVLGWKSKRLLS SIW SS+S SSSSS SDSE
Sbjct: 456 FNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

[Close]

BLAST of MC04g1256 vs. ExPASy TrEMBL
Match: A0A0A0KDX4 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 SV=1)

HSP 1 Score: 593 bits (1528), Expect = 2.27e-208
Identity = 323/458 (70.52%), Postives = 356/458 (77.73%), Query Frame = 0


Query: 2   YCSPDADWEAFPGA-GDFHDLIDSVMDADVPTYAAEVDGSSNCNSEEEESTEAEDQEEEL 61
           Y SP  DWE F G   DF D+ DS +  + P +                 TE  D+E+E 
Sbjct: 36  YGSPIVDWETFSGTHNDFLDVFDSTICIENPPFL----------------TEDRDEEDEF 95

Query: 62  KGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLL 121
           KGLRLYHLL AAADA+ G+HKS DLAHVIL+RLNELVSPSHGTNLERL AYY QA Q LL
Sbjct: 96  KGLRLYHLLTAAADAVLGDHKSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLL 155

Query: 122 ECTAGVGKSHHHLH----RDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDR 181
           +      K+HHH H    RDDHTP DV+AAFQLLQEMSPYVKFGHFTANQAILEAV +DR
Sbjct: 156 DSAPVANKTHHHNHHIHQRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDR 215

Query: 182 RVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRL 241
           RVHIVDYDIMEGIQWASLMQAFVS    P APHLRITAISRG +  RRSIGTVQETGRRL
Sbjct: 216 RVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGAN-GRRSIGTVQETGRRL 275

Query: 242 VAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFL 301
           VAFAASIGQPFSFHQCKLDSDESFR +GLKLVKGEA+VVNCMLHLPHF +R+PESI SFL
Sbjct: 276 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFL 335

Query: 302 RGAEALSPRVVCLVEEEIGHGI---GDYKVEFVESLERYSAIYESLEVGLPMNNRGRALV 361
            GA++LSPR+V LVEEEIGHG    GDYKV+F++SLERYSAIY+SLE  +PM NR RALV
Sbjct: 336 SGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALV 395

Query: 362 ERVFLGPQIWGSVGLMGQPRTAAGS------WGEWLGMMGMKAAPISFANHCQARLLVGL 421
           ERVFLGP+I  ++  +GQ R +         WGE L  MG+K   ISFANHCQARLL+GL
Sbjct: 396 ERVFLGPRISATLRRIGQRRWSTAEMEDNCLWGEKLEKMGLKTVKISFANHCQARLLLGL 455

Query: 422 FNDGYIVEELSNNKLVLGWKSKRLLSASIWASSSSPSS 445
           FNDGY VEEL NNKLVLGWKSKRLLS SIW SSSS SS
Sbjct: 456 FNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSSSSSS 473

[Close]

BLAST of MC04g1256 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 411.8 bits (1057), Expect = 7.3e-115
Identity = 233/452 (51.55%), Postives = 296/452 (65.49%), Query Frame = 0


Query: 6   DADWEAFPGAGDFHDLIDSVMDADVPTYAAEV-------DGSSNCNSEEEESTEA----- 65
           D DW+      DF D+I+S+M  +      E        D    CNS     + A     
Sbjct: 37  DIDWDC-----DFRDVIESIMGDEGAMMEPESEAVPMLHDQEGLCNSASTGLSVADGVSF 96

Query: 66  -EDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYY 125
            E + +E KGLRL HLL+AAADA  G +KSR+L  VIL RL +LVSP   TN+ERLAA++
Sbjct: 97  GEPKTDESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHF 156

Query: 126 TQALQGLLECTAGVGKSHHHLHRDD-HTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEA 185
           T  L  LLE  + +       HRDD +   DV++AF+LLQ MSPYV FG+ TA QAILEA
Sbjct: 157 TNGLSKLLERDSVLCPQQ---HRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEA 216

Query: 186 VGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQE 245
           V  +RR+HIVDYDI EG+QWASLMQA VSR  GP A HLRITA+SR  +  ++S+  VQE
Sbjct: 217 VKYERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSR-ATNGKKSVAAVQE 276

Query: 246 TGRRLVAFAASIGQPFSFHQCKLDSDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPES 305
           TGRRL AFA SIGQPFS+  CKLD++ +F  + LKLV+GEAVV+NCMLHLP F H+TP S
Sbjct: 277 TGRRLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSS 336

Query: 306 IGSFLRGAEALSPRVVCLVEEEIG-HGIGDYKVEFVESLERYSAIYESLEVGLPMNNRGR 365
           + SFL  A+ L+P++V LV EE+G  G   +   F++ L ++SAI++SLE GL + N  R
Sbjct: 337 VISFLSEAKTLNPKLVTLVHEEVGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPAR 396

Query: 366 ALVERVFLGPQI--W-GSVGLMGQPRTAAGSWGEWLGMMGMKAAPISFANHCQARLLVGL 425
             VERVF+GP +  W   +        +  SW +WL   G K   +SF N CQA+LL+ L
Sbjct: 397 GFVERVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSL 456

Query: 426 FNDGYIVEELSNNKLVLGWKSKRLLSASIWAS 440
           FNDG+ VEEL  N LVLGWKS+RL+SAS WAS
Sbjct: 457 FNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

[Close]

BLAST of MC04g1256 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 192.2 bits (487), Expect = 9.1e-49
Identity = 136/400 (34.00%), Postives = 211/400 (52.75%), Query Frame = 0


Query: 48  EESTEAEDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLER 107
           E   E + Q+++ +GL L  LL+  A+A+  ++     A+ +LL +++L +P +GT+ +R
Sbjct: 273 ERKEEIKRQKQDEEGLHLLTLLLQCAEAVSADNLEE--ANKLLLEISQLSTP-YGTSAQR 332

Query: 108 LAAYYTQALQG-LLECTAGVGKSHHHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQ 167
           +AAY+++A+   LL    G+  +             +V+AFQ+   +SP VKF HFTANQ
Sbjct: 333 VAAYFSEAMSARLLNSCLGIYAALPSRWMPQTHSLKMVSAFQVFNGISPLVKFSHFTANQ 392

Query: 168 AILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSI 227
           AI EA   +  VHI+D DIM+G+QW  L     SR  GP  PH+R+T +         S+
Sbjct: 393 AIQEAFEKEDSVHIIDLDIMQGLQWPGLFHILASRPGGP--PHVRLTGLG-------TSM 452

Query: 228 GTVQETGRRLVAFAASIGQPFSFHQCKL-DSDESFRAAGLKLVKGEAVVVNCMLHLPHFR 287
             +Q TG+RL  FA  +G PF F  C L +   +     L + K EAV V+    L H  
Sbjct: 453 EALQATGKRLSDFADKLGLPFEF--CPLAEKVGNLDTERLNVRKREAVAVH---WLQHSL 512

Query: 288 HRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESLEVGLPM 347
           +    S    L   + L+P+VV +VE+++ H  G +   FVE++  YSA+++SL      
Sbjct: 513 YDVTGSDAHTLWLLQRLAPKVVTVVEQDLSHA-GSFLGRFVEAIHYYSALFDSLGASYGE 572

Query: 348 NNRGRALVERVFLGPQIWGSVGLMGQPRTAA---GSWGEWLGMMGMKAAPISFANHCQAR 407
            +  R +VE+  L  +I   + + G  R+      SW E +   G K   ++     QA 
Sbjct: 573 ESEERHVVEQQLLSKEIRNVLAVGGPSRSGEVKFESWREKMQQCGFKGISLAGNAATQAT 632

Query: 408 LLVGLF-NDGYIVEELSNNKLVLGWKSKRLLSASIWASSS 442
           LL+G+F +DGY + +  N  L LGWK   LL+AS W   S
Sbjct: 633 LLLGMFPSDGYTLVD-DNGTLKLGWKDLSLLTASAWTPRS 653

[Close]

BLAST of MC04g1256 vs. TAIR 10
Match: AT1G55580.1 (GRAS family transcription factor )

HSP 1 Score: 176.4 bits (446), Expect = 5.2e-44
Identity = 137/447 (30.65%), Postives = 202/447 (45.19%), Query Frame = 0


Query: 40  SSNCNSEEEESTEAED------QEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRL 99
           SS+ +SE+  +T  E+                +HL      A     +S   A   LL +
Sbjct: 7   SSSSSSEDATATTTENPPPLCIASSSAATSASHHLRRLLFTAANFVSQSNFTAAQNLLSI 66

Query: 100 NELVSPSHGTNLERLAAYYTQALQGLLE--------------------------CTAGVG 159
             L S  HG + ERL   +T+AL   +                            T+ V 
Sbjct: 67  LSLNSSPHGDSTERLVHLFTKALSVRINRQQQDQTAETVATWTTNEMTMSNSTVFTSSVC 126

Query: 160 KSHHHLHRDDHTPGDVVAAFQL-LQEMSPYVKFGHFTANQAILEA--VGDDRRVHIVDYD 219
           K    L R  +   D  + + L L +++P+++FGH TANQAIL+A    D+  +HI+D D
Sbjct: 127 K-EQFLFRTKNNNSDFESCYYLWLNQLTPFIRFGHLTANQAILDATETNDNGALHILDLD 186

Query: 220 IMEGIQWASLMQAFVSRKDGP--PAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAAS 279
           I +G+QW  LMQA   R   P  P P LRIT          R +  +  TG RL  FA S
Sbjct: 187 ISQGLQWPPLMQALAERSSNPSSPPPSLRITGCG-------RDVTGLNRTGDRLTRFADS 246

Query: 280 IGQPFSFHQCKLDSDE------SFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFL 339
           +G  F FH   +  ++        R   L  V+GE + VNC+  L    +   + IG FL
Sbjct: 247 LGLQFQFHTLVIVEEDLAGLLLQIRLLALSAVQGETIAVNCVHFLHKIFNDDGDMIGHFL 306

Query: 340 RGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERV 399
              ++L+ R+V + E E  HG   +   F E+++ Y AI++SLE  LP N+R R  +E+ 
Sbjct: 307 SAIKSLNSRIVTMAEREANHGDHSFLNRFSEAVDHYMAIFDSLEATLPPNSRERLTLEQR 366

Query: 400 FLGPQIWGSVGLMGQPRTAAGS----WGEWLGMMGMKAAPISFANHCQARLLVGLF--ND 438
           + G +I   V      R         W E +   G    PI      QA+LL+ L   ++
Sbjct: 367 WFGKEILDVVAAEETERKQRHRRFEIWEEMMKRFGFVNVPIGSFALSQAKLLLRLHYPSE 426

[Close]

BLAST of MC04g1256 vs. TAIR 10
Match: AT5G48150.1 (GRAS family transcription factor )

HSP 1 Score: 172.9 bits (437), Expect = 5.7e-43
Identity = 117/362 (32.32%), Postives = 187/362 (51.66%), Query Frame = 0


Query: 85  LAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVGKS-HHHLHR-DDHTPGD 144
           +AH ++ +L ++VS S G  ++RL AY    L+GL+   A  G S +  L+R  +    +
Sbjct: 137 MAHSMMEKLRQMVSVS-GEPIQRLGAY---LLEGLVAQLASSGSSIYKALNRCPEPASTE 196

Query: 145 VVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRK 204
           +++   +L E+ PY KFG+ +AN AI EA+ ++ RVHI+D+ I +G QW +L+QAF +R 
Sbjct: 197 LLSYMHILYEVCPYFKFGYMSANGAIAEAMKEENRVHIIDFQIGQGSQWVTLIQAFAARP 256

Query: 205 DGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRA 264
            GP  P +RIT I    S   R  G +   G RL   A     PF F+   +   E  + 
Sbjct: 257 GGP--PRIRITGIDDMTSAYARG-GGLSIVGNRLAKLAKQFNVPFEFNSVSVSVSE-VKP 316

Query: 265 AGLKLVKGEAVVVN---CMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIG 324
             L +  GEA+ VN    + H+P     T       LR  ++LSP+VV LVE+E      
Sbjct: 317 KNLGVRPGEALAVNFAFVLHHMPDESVSTENHRDRLLRMVKSLSPKVVTLVEQESNTNTA 376

Query: 325 DYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGLMGQPRT----A 384
            +   F+E++  Y+A++ES++V LP +++ R  VE+  L   +   +   G  R      
Sbjct: 377 AFFPRFMETMNYYAAMFESIDVTLPRDHKQRINVEQHCLARDVVNIIACEGADRVERHEL 436

Query: 385 AGSWGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNKLVLGWKSKRLLSAS 438
            G W    GM G    P+S   +   + L+  ++D Y +EE  +  L LGW  + L+++ 
Sbjct: 437 LGKWRSRFGMAGFTPYPLSPLVNSTIKSLLRNYSDKYRLEE-RDGALYLGWMHRDLVASC 489

[Close]

BLAST of MC04g1256 vs. TAIR 10
Match: AT5G48150.2 (GRAS family transcription factor )

HSP 1 Score: 172.9 bits (437), Expect = 5.7e-43
Identity = 117/362 (32.32%), Postives = 187/362 (51.66%), Query Frame = 0


Query: 85  LAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVGKS-HHHLHR-DDHTPGD 144
           +AH ++ +L ++VS S G  ++RL AY    L+GL+   A  G S +  L+R  +    +
Sbjct: 137 MAHSMMEKLRQMVSVS-GEPIQRLGAY---LLEGLVAQLASSGSSIYKALNRCPEPASTE 196

Query: 145 VVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRK 204
           +++   +L E+ PY KFG+ +AN AI EA+ ++ RVHI+D+ I +G QW +L+QAF +R 
Sbjct: 197 LLSYMHILYEVCPYFKFGYMSANGAIAEAMKEENRVHIIDFQIGQGSQWVTLIQAFAARP 256

Query: 205 DGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRA 264
            GP  P +RIT I    S   R  G +   G RL   A     PF F+   +   E  + 
Sbjct: 257 GGP--PRIRITGIDDMTSAYARG-GGLSIVGNRLAKLAKQFNVPFEFNSVSVSVSE-VKP 316

Query: 265 AGLKLVKGEAVVVN---CMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIG 324
             L +  GEA+ VN    + H+P     T       LR  ++LSP+VV LVE+E      
Sbjct: 317 KNLGVRPGEALAVNFAFVLHHMPDESVSTENHRDRLLRMVKSLSPKVVTLVEQESNTNTA 376

Query: 325 DYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGLMGQPRT----A 384
            +   F+E++  Y+A++ES++V LP +++ R  VE+  L   +   +   G  R      
Sbjct: 377 AFFPRFMETMNYYAAMFESIDVTLPRDHKQRINVEQHCLARDVVNIIACEGADRVERHEL 436

Query: 385 AGSWGEWLGMMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNKLVLGWKSKRLLSAS 438
            G W    GM G    P+S   +   + L+  ++D Y +EE  +  L LGW  + L+++ 
Sbjct: 437 LGKWRSRFGMAGFTPYPLSPLVNSTIKSLLRNYSDKYRLEE-RDGALYLGWMHRDLVASC 489

The following BLAST results are available for this feature:

BLAST of MC04g1256 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2PEG7	2.4e-139	57.85	Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 ...	[more]
Q5NE24	2.4e-139	54.93	Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE...	[more]
Q84Q92	5.5e-115	50.30	Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SUF5	1.0e-113	51.55	Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1	[more]
Q2RB59	7.3e-51	34.54	Protein SCARECROW 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SCR1 PE=1 SV=1	[more]

BLAST of MC04g1256 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022993952.1	2.02e-218	73.45	nodulation-signaling pathway 2 protein [Cucurbita maxima]	[more]
XP_023551167.1	6.68e-217	73.45	nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6578977.1	1.34e-216	73.01	Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. s...	[more]
KAG7016501.1	2.71e-216	72.79	Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022938560.1	4.45e-215	72.57	nodulation-signaling pathway 2 protein [Cucurbita moschata]	[more]

BLAST of MC04g1256 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JUC4	9.76e-219	73.45	nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC1114898...	[more]
A0A6J1FK48	2.16e-215	72.57	nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7V767	4.64e-211	70.39	Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BE54	4.64e-211	70.39	nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 P...	[more]
A0A0A0KDX4	2.27e-208	70.52	GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 S...	[more]

BLAST of MC04g1256 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G08250.1	7.3e-115	51.55	GRAS family transcription factor	[more]
AT3G54220.1	9.1e-49	34.00	GRAS family transcription factor	[more]
AT1G55580.1	5.2e-44	30.65	GRAS family transcription factor	[more]
AT5G48150.1	5.7e-43	32.32	GRAS family transcription factor	[more]
AT5G48150.2	5.7e-43	32.32	GRAS family transcription factor	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 43..63
IPR005202	Transcription factor GRAS	PFAM	PF03514	GRAS	coord: 66..437 e-value: 2.2E-95 score: 319.8
IPR005202	Transcription factor GRAS	PANTHER	PTHR31636	OSJNBA0084A10.13 PROTEIN-RELATED	coord: 4..439
IPR005202	Transcription factor GRAS	PROSITE	PS50985	GRAS	coord: 56..438 score: 69.805054
IPR030028	Scarecrow-like protein 26/nodulation signalling pathway 2	PANTHER	PTHR31636:SF25	SCARECROW-LIKE PROTEIN 26	coord: 4..439

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g1256.1	MC04g1256.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding