MC04g0662 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0662
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	CYSTM domain-containing protein
Location	MC04: 6144370 .. 6144835 (+)
RNA-Seq Expression	MC04g0662
Synteny	MC04g0662

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGATCCAAAATATGGCTATCCTTACCCACCTCCTCAAGGTAAATTTTCCACACCAATCTTCACTAATCTGTTTTCGAATTCGAAATTTAGTGAATTTGAAATTTCGATCGGTAACAAAGAAGATTACCGATCATGTCTTTACTGTCGAACTATGCTTTCCAGTTGTTTGTTGCGGTGAAATGTTTTCAGTGTTGTGATACTTATTTGGCAGGAGGTTATTATCAAGGGCCGCCAGTGATGGCGCCACCGCAATACGCGGTGCCACCACCGAAAAGGCAGCCAGGCTTCTTGGAGGGATGGTATGCCAAAGCAACTCTCGGTCTCGATTTCGGTTTCGATTTTGGTTTTGTTTTGACAGAGGTTGGGTTTTGTTTGGTTGTTGTTGTTGCAGCCTTGCTGCTCTGTGCTGCTGCTGTCTCCTTGATGAGTGCTGCTGTGACCCTTCCATCATATTTCTTGCT

mRNA sequence

ATGAGTGATCCAAAATATGGCTATCCTTACCCACCTCCTCAAGGAGGTTATTATCAAGGGCCGCCAGTGATGGCGCCACCGCAATACGCGGTGCCACCACCGAAAAGGCAGCCAGGCTTCTTGGAGGGATGCCTTGCTGCTCTGTGCTGCTGCTGTCTCCTTGATGAGTGCTGCTGTGACCCTTCCATCATATTTCTTGCT

Coding sequence (CDS)

ATGAGTGATCCAAAATATGGCTATCCTTACCCACCTCCTCAAGGAGGTTATTATCAAGGGCCGCCAGTGATGGCGCCACCGCAATACGCGGTGCCACCACCGAAAAGGCAGCCAGGCTTCTTGGAGGGATGCCTTGCTGCTCTGTGCTGCTGCTGTCTCCTTGATGAGTGCTGCTGTGACCCTTCCATCATATTTCTTGCT

Protein sequence

MSDPKYGYPYPPPQGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCCDPSIIFLA

Homology

[Close]

BLAST of MC04g0662 vs. ExPASy Swiss-Prot
Match: Q8S8M0 (Cysteine-rich and transmembrane domain-containing protein WIH2 OS=Arabidopsis thaliana OX=3702 GN=WIH2 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.6e-09
Identity = 39/65 (60.00%), Postives = 40/65 (61.54%), Query Frame = 0


Query: 4  PKYGYP---YPP---PQGGY-YQGPPVMAPPQYAVPPPKRQ----PGFLEGCLAALCCCC 58
          P  GYP   YPP   PQ GY  QG P    PQY  PP  +Q    PGFLEGCLAALCCCC
Sbjct: 33 PPQGYPQQGYPPQGYPQQGYPQQGYPPPYAPQYPPPPQHQQQQSSPGFLEGCLAALCCCC 92

[Close]

BLAST of MC04g0662 vs. ExPASy Swiss-Prot
Match: Q9FJW3 (Cysteine-rich and transmembrane domain-containing protein WIH1 OS=Arabidopsis thaliana OX=3702 GN=WIH1 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.5e-08
Identity = 37/63 (58.73%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 4  PKYGYP---YPP---PQGGYYQGPPVMA--PPQYA-VPPPKRQPGFLEGCLAALCCCCLL 58
          PK GYP   YPP   P  GY QG P     PPQY+  P  K+  G LEGCLAALCCCCLL
Sbjct: 19 PKDGYPPAGYPPAGYPPPGYAQGYPAQGYPPPQYSQAPQQKQNAGMLEGCLAALCCCCLL 78

[Close]

BLAST of MC04g0662 vs. ExPASy Swiss-Prot
Match: Q8LCL8 (Cysteine-rich and transmembrane domain-containing protein B OS=Arabidopsis thaliana OX=3702 GN=At3g57160 PE=3 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 6.8e-06
Identity = 32/67 (47.76%), Postives = 37/67 (55.22%), Query Frame = 0


Query: 4   PKYGYPYP--------PPQGGYYQGPPVMAPPQYAVPPPKRQ-----PGFLEGCLAALCC 58
           P+ GYP P        PPQG   QG P    PQ   PP ++Q     PG LEGC+AALCC
Sbjct: 34  PQQGYPPPQGYPQQGYPPQGYPPQGYPEQGYPQQGYPPQQQQQQKHSPGMLEGCIAALCC 93

[Close]

BLAST of MC04g0662 vs. NCBI nr
Match: KAG6571885.1 (hypothetical protein SDJN03_28613, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011569.1 hypothetical protein SDJN02_26475 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 135 bits (340), Expect = 5.89e-40
Identity = 60/64 (93.75%), Postives = 61/64 (95.31%), Query Frame = 0


Query: 1  MSDPKYGYPYPPPQGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKY YPYPPP GGYYQGPPVMAPPQYA PPPKRQPGFLEGCLAALCCCCLLDECCCD
Sbjct: 1  MSDPKYAYPYPPPPGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60

Query: 61 PSII 64
          PSI+
Sbjct: 61 PSIL 64

[Close]

BLAST of MC04g0662 vs. NCBI nr
Match: KAF9609677.1 (hypothetical protein IFM89_017856 [Coptis chinensis])

HSP 1 Score: 129 bits (324), Expect = 1.63e-37
Identity = 58/65 (89.23%), Postives = 60/65 (92.31%), Query Frame = 0


Query: 1  MSDPKYGYPYPPPQGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          M+DPKY YPYP P  GYYQGPPVMAPPQYA PPP+RQPGFLEGCLAALCCCCLLDECCCD
Sbjct: 1  MNDPKYAYPYPYPAQGYYQGPPVMAPPQYAAPPPRRQPGFLEGCLAALCCCCLLDECCCD 60

Query: 61 PSIIF 65
          PSIIF
Sbjct: 61 PSIIF 65

[Close]

BLAST of MC04g0662 vs. NCBI nr
Match: PQP99874.1 (uncharacterized protein Pyn_05607 [Prunus yedoensis var. nudiflora] >PQQ01249.1 uncharacterized protein Pyn_09821 [Prunus yedoensis var. nudiflora])

HSP 1 Score: 128 bits (321), Expect = 4.81e-37
Identity = 57/66 (86.36%), Postives = 62/66 (93.94%), Query Frame = 0


Query: 1  MSDPKYGYPYPPP-QGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCC 60
          MSDPKYGYPYP   QGGYYQGPPVMAPPQYA PPP+R+PGFLEGCLAALCCCCL+DECCC
Sbjct: 1  MSDPKYGYPYPAQGQGGYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCC 60

Query: 61 DPSIIF 65
          DPS++F
Sbjct: 61 DPSVLF 66

[Close]

BLAST of MC04g0662 vs. NCBI nr
Match: CAB4283958.1 (unnamed protein product [Prunus armeniaca] >CAB4314388.1 unnamed protein product [Prunus armeniaca])

HSP 1 Score: 127 bits (318), Expect = 1.38e-36
Identity = 56/66 (84.85%), Postives = 62/66 (93.94%), Query Frame = 0


Query: 1  MSDPKYGYPYPPP-QGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCC 60
          M+DPKYGYPYP   QGGYYQGPPVMAPPQYA PPP+R+PGFLEGCLAALCCCCL+DECCC
Sbjct: 1  MNDPKYGYPYPAQGQGGYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCC 60

Query: 61 DPSIIF 65
          DPS++F
Sbjct: 61 DPSVLF 66

[Close]

BLAST of MC04g0662 vs. NCBI nr
Match: PIA51886.1 (hypothetical protein AQUCO_01000039v1 [Aquilegia coerulea])

HSP 1 Score: 126 bits (316), Expect = 2.57e-36
Identity = 57/66 (86.36%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 1  MSDPKYGYPYPPPQGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYGYPYP    GYYQGPPVMAPPQYA PPP+R+PGFLEGCLAALCCCCL+DECCCD
Sbjct: 1  MSDPKYGYPYP--AQGYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCCD 60

Query: 61 PSIIFL 66
          PSIIF+
Sbjct: 61 PSIIFI 64

[Close]

BLAST of MC04g0662 vs. ExPASy TrEMBL
Match: A0A314Y8T5 (CYSTM domain-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_05607 PE=3 SV=1)

HSP 1 Score: 128 bits (321), Expect = 2.33e-37
Identity = 57/66 (86.36%), Postives = 62/66 (93.94%), Query Frame = 0


Query: 1  MSDPKYGYPYPPP-QGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCC 60
          MSDPKYGYPYP   QGGYYQGPPVMAPPQYA PPP+R+PGFLEGCLAALCCCCL+DECCC
Sbjct: 1  MSDPKYGYPYPAQGQGGYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCC 60

Query: 61 DPSIIF 65
          DPS++F
Sbjct: 61 DPSVLF 66

[Close]

BLAST of MC04g0662 vs. ExPASy TrEMBL
Match: A0A6J5XT78 (CYSTM domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS39353 PE=3 SV=1)

HSP 1 Score: 127 bits (318), Expect = 6.69e-37
Identity = 56/66 (84.85%), Postives = 62/66 (93.94%), Query Frame = 0


Query: 1  MSDPKYGYPYPPP-QGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCC 60
          M+DPKYGYPYP   QGGYYQGPPVMAPPQYA PPP+R+PGFLEGCLAALCCCCL+DECCC
Sbjct: 1  MNDPKYGYPYPAQGQGGYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCC 60

Query: 61 DPSIIF 65
          DPS++F
Sbjct: 61 DPSVLF 66

[Close]

BLAST of MC04g0662 vs. ExPASy TrEMBL
Match: A0A2G5E805 (CYSTM domain-containing protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_01000039v1 PE=3 SV=1)

HSP 1 Score: 126 bits (316), Expect = 1.24e-36
Identity = 57/66 (86.36%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 1  MSDPKYGYPYPPPQGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYGYPYP    GYYQGPPVMAPPQYA PPP+R+PGFLEGCLAALCCCCL+DECCCD
Sbjct: 1  MSDPKYGYPYP--AQGYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCCD 60

Query: 61 PSIIFL 66
          PSIIF+
Sbjct: 61 PSIIFI 64

[Close]

BLAST of MC04g0662 vs. ExPASy TrEMBL
Match: A0A540N3X6 (CYSTM domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_008668 PE=3 SV=1)

HSP 1 Score: 125 bits (314), Expect = 2.65e-36
Identity = 60/67 (89.55%), Postives = 63/67 (94.03%), Query Frame = 0


Query: 1  MSDPKYGYPYPPPQGGYYQGPPVMAPPQY-AVPPPKRQPGFLEGCLAALCCCCLLDECCC 60
          M++PKYGYPYPPPQG Y QGPPVMAPPQY A PPPKR+PGFLEGCLAALCCCCLLDECCC
Sbjct: 1  MNEPKYGYPYPPPQGPY-QGPPVMAPPQYNAAPPPKREPGFLEGCLAALCCCCLLDECCC 60

Query: 61 DPSIIFL 66
          DPSIIFL
Sbjct: 61 DPSIIFL 66

[Close]

BLAST of MC04g0662 vs. ExPASy TrEMBL
Match: B9SMJ5 (CYSTM domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_0230320 PE=3 SV=1)

HSP 1 Score: 125 bits (313), Expect = 3.57e-36
Identity = 57/67 (85.07%), Postives = 61/67 (91.04%), Query Frame = 0


Query: 1  MSDPKYGYPYPPPQGGYYQGPPVMAPPQYAVPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKY YPYP    GYYQGPPVMAPPQYA PPP+RQPGFLEGCLAALCCCCL+DECCCD
Sbjct: 1  MSDPKYAYPYP--AQGYYQGPPVMAPPQYAAPPPRRQPGFLEGCLAALCCCCLIDECCCD 60

Query: 61 PSIIFLA 67
          PSIIF++
Sbjct: 61 PSIIFVS 65

[Close]

BLAST of MC04g0662 vs. TAIR 10
Match: AT4G33660.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 89.7 bits (221), Expect = 9.4e-19
Identity = 49/74 (66.22%), Postives = 52/74 (70.27%), Query Frame = 0


Query: 1  MSDPKYGYPYPPPQGGYYQG--PPVMAPPQY--------AVPPPKRQPGFLEGCLAALCC 60
          MSDPKY YPYP P G Y QG  PPV  PPQY          PPP R+ GFLEG LAALCC
Sbjct: 1  MSDPKYAYPYPAP-GNYPQGPPPPVGVPPQYYPPPPPPPPPPPPPRKVGFLEGLLAALCC 60

Query: 61 CCLLDECCCDPSII 65
          CCL+DECCCDP+II
Sbjct: 61 CCLVDECCCDPTII 73

[Close]

BLAST of MC04g0662 vs. TAIR 10
Match: AT2G41420.1 (proline-rich family protein )

HSP 1 Score: 60.5 bits (145), Expect = 6.1e-10
Identity = 39/65 (60.00%), Postives = 40/65 (61.54%), Query Frame = 0


Query: 4  PKYGYP---YPP---PQGGY-YQGPPVMAPPQYAVPPPKRQ----PGFLEGCLAALCCCC 58
          P  GYP   YPP   PQ GY  QG P    PQY  PP  +Q    PGFLEGCLAALCCCC
Sbjct: 33 PPQGYPQQGYPPQGYPQQGYPQQGYPPPYAPQYPPPPQHQQQQSSPGFLEGCLAALCCCC 92

[Close]

BLAST of MC04g0662 vs. TAIR 10
Match: AT5G67600.1 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G49845.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 58.9 bits (141), Expect = 1.8e-09
Identity = 37/63 (58.73%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 4  PKYGYP---YPP---PQGGYYQGPPVMA--PPQYA-VPPPKRQPGFLEGCLAALCCCCLL 58
          PK GYP   YPP   P  GY QG P     PPQY+  P  K+  G LEGCLAALCCCCLL
Sbjct: 19 PKDGYPPAGYPPAGYPPPGYAQGYPAQGYPPPQYSQAPQQKQNAGMLEGCLAALCCCCLL 78

[Close]

BLAST of MC04g0662 vs. TAIR 10
Match: AT3G49845.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: root; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 50.8 bits (120), Expect = 4.8e-07
Identity = 36/86 (41.86%), Postives = 40/86 (46.51%), Query Frame = 0


Query: 4   PKYGYP---YPPPQGGY-----------------------YQGPPVMAPPQYAVPPPKRQ 58
           P+ GYP   YPPPQ GY                       YQGPP   PP Y   PPK +
Sbjct: 41  PQAGYPPAGYPPPQQGYGQGYPAQGYPPPQYPQGHPPQYPYQGPP---PPHYGQAPPKNK 100

[Close]

BLAST of MC04g0662 vs. TAIR 10
Match: AT1G12810.1 (proline-rich family protein )

HSP 1 Score: 43.1 bits (100), Expect = 1.0e-04
Identity = 33/86 (38.37%), Postives = 36/86 (41.86%), Query Frame = 0


Query: 7   GYPYPPPQGGY-----------YQGPPVMA--PPQYAVPPPKRQP--------------- 59
           GYP P P GGY           YQG       P Q+  PPP   P               
Sbjct: 43  GYPPPQPYGGYPPPSSRPYEGGYQGYFAGGGYPHQHHGPPPPPPPQNYDHCHHDHHHYQD 102

The following BLAST results are available for this feature:

BLAST of MC04g0662 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8M0	8.6e-09	60.00	Cysteine-rich and transmembrane domain-containing protein WIH2 OS=Arabidopsis th...	[more]
Q9FJW3	2.5e-08	58.73	Cysteine-rich and transmembrane domain-containing protein WIH1 OS=Arabidopsis th...	[more]
Q8LCL8	6.8e-06	47.76	Cysteine-rich and transmembrane domain-containing protein B OS=Arabidopsis thali...	[more]

BLAST of MC04g0662 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571885.1	5.89e-40	93.75	hypothetical protein SDJN03_28613, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAF9609677.1	1.63e-37	89.23	hypothetical protein IFM89_017856 [Coptis chinensis]	[more]
PQP99874.1	4.81e-37	86.36	uncharacterized protein Pyn_05607 [Prunus yedoensis var. nudiflora] >PQQ01249.1 ...	[more]
CAB4283958.1	1.38e-36	84.85	unnamed protein product [Prunus armeniaca] >CAB4314388.1 unnamed protein product...	[more]
PIA51886.1	2.57e-36	86.36	hypothetical protein AQUCO_01000039v1 [Aquilegia coerulea]	[more]

BLAST of MC04g0662 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A314Y8T5	2.33e-37	86.36	CYSTM domain-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN...	[more]
A0A6J5XT78	6.69e-37	84.85	CYSTM domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS3935...	[more]
A0A2G5E805	1.24e-36	86.36	CYSTM domain-containing protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_0100003...	[more]
A0A540N3X6	2.65e-36	89.55	CYSTM domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_008668 PE=3 ...	[more]
B9SMJ5	3.57e-36	85.07	CYSTM domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_0230320 PE=3...	[more]

BLAST of MC04g0662 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33660.1	9.4e-19	66.22	unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -...	[more]
AT2G41420.1	6.1e-10	60.00	proline-rich family protein	[more]
AT5G67600.1	1.8e-09	58.73	unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 22 plant structures;...	[more]
AT3G49845.1	4.8e-07	41.86	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G12810.1	1.0e-04	38.37	proline-rich family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR028144	Cysteine-rich transmembrane CYSTM domain	PFAM	PF12734	CYSTM	coord: 12..58 e-value: 2.2E-15 score: 56.6
IPR044850	Cysteine-rich and transmembrane domain-containing protein WIH1/2-like	PANTHER	PTHR31568	RCG49325, ISOFORM CRA_A	coord: 1..66
None	No IPR available	PANTHER	PTHR31568:SF105	CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN A-LIKE	coord: 1..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0662.1	MC04g0662.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane