MC04g0500 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0500
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
LocationMC04: 4191603 .. 4195674 (-)
RNA-Seq ExpressionMC04g0500
SyntenyMC04g0500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAAATGGCGTTACGAAACGAACCATAGAAAAAGCAAGACCTTTTTCGTAATTTTAAATGCGATCCCGGAATTAGGATCCTCCACTGATTCTACTTGACTAAACGCCAAAACTACGAATAAAAATAATAATAATAAATTTTTAGTGAACCATAAAATAAATCATAATAATATTAACAATAATAATTTCCACTGTAATTTTATCAATTTATATATAAAAAAAAAAAGAAATAGTTGATTCAAAGATAAATGGAGGGAGAAGAAGCAGAGGTCGTTTGTGAGTGGTAATTGAGCTCAAAACTAGTCTCTCCCATGGCGTTCTCTCTCCAACTTTGTCGTCTTCCTATTCGTACGGATGCGGCCTTGCCGGAGAATTTCGCCGGGATTCGAGCACTCCGGTACAGGAGGCCTCTCTCCGTGAGATGCGATGCTGATGCTTCTTCTTCTTCGCCTTCGGTGGCTGTGGATTCTGATTTTGACGCCAAGGTGTTCCGGAAGAATTTGACGAGGAGCGACAATTACAACCGCAAAGGGTTTGGTCACAAGGAGGAGACTCTGGCGCTCATGGATCGCGAGTATACCAGTGAGTCTCCACTGAATTTTACGAATTGAGGAAAAATATTATGAATTCCATGTTCTATTACCTGCTGCTCCACTTCCGATTTGGCTTCTCGAGGCCAGAAACTATGGATTAAGGGGGAAAAAGCTTTATCACTTGAATTTTTCAACTTGTTCAAAAATAAAAGTTGTTGGGTAGCTCGTGTGTACTTAATTCTATGTTGAAATTGCAGGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTGAAACTGGCGGAAGCGTATGGATTCTGTTGGGGAGTTGAGCGGGCTGTTCAGATTGCCTATGAAGCGAGAAAGCAATTTCCAGAAGAGAAAATATGGATAACCAATGAAATTATTCACAACCCAACTGTTAACAAGGTAACGATCAATTTTGTGCCGGTTTCTTTAGATGTCTCAAATTTTGAGTTTTAATCTGGTAAATTTGGTGGTGCTTGATGTGTTCTTGTCCACTAACAAATTTTTAGCATCTGTTCTACTCTCAGTCAGATGAGATTCTTTTATCACTTTCTGGGATCTCTCTCTTTTTCTCTGACTCAATTATGCATCTTTTGTATTGAGGGAGTTCGAATTTTCCACTTCTAGTTTATCGTAAACTAGCATGCTTTGTGGTCTCTCTTTTATTCGTCTAATGACATGATATGCTCCAGCTTATTATTCCCCGGAAAACACGATCTATTTCTTGTTTTAATTTTTGTTTTCCATTTCCATCTCTTGACGAGTCTGAACTGTGATTTGGAAATTTTGCAAGATAGTCTTCCAGTTGCAGACCCAGTCCAATGAATATGGTTTTCAGTCTGGTGACTTGACTATTAGGGATGAATTAGACTTTTGACCTTCCAGTGTTTGGGGAGAATGCAATTTTCGCCCCTTTTTTTGTGGCCTCTAAGATATATCTTGTTTATTTTATTTTTAATTCTAATTTTTCCATCTTCTGTATCTGACTTGTGTGGGTAAGATGGTCTTGTCGACCTTCTAGTGTTTGGATTGGAAGCAGTAAAAGTCTTGCACCAGTACTTGTCTTTTTTTTTCCCATTTTAGATCTTCAACTTTATCTTTATTGCTTGCTGTTCTCTTCCTAGAAATTTGAAACACTACTTCAGATTTCCTTACTGTCTTTTTCCAAACAGGAGAACATCCTGATGTATGAGTCGAGTTCAGAAGTTTTAGAACTTAATTGTTTGTCGAATTTCATGGAATCCTTCATACAGCGCCTAATTTCCTTACTACTAATAGAAAATAAATGGCATTAAAATTTTCGAATTATACATGTAGCGCTTAGAGGATATGAAAGTTGAAAACATTCCACTCGAGGAAGGAAAGAAACAGTTTGAAGTGGTTGACAAGGGTGATGTTGTGATTTTGCCTGCATTTGGAGCTGCAGTGGACGAGATGTTGACATTGAGTGAAAAAAGTGTGCAAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTATTGTTTTGTTACCTTTTCTCTTCTTTGCTATACATTCTGATATACCGACAGTAATGCTATACTGCAGTTAGTATTATGTATGTTACTTCTGAGAAAAATTATCTCTTGATGTATGGTTTTGTTGTCTGTACAACTTTTGAAGGTTTGGAATGTTGTTGAAAAGCACAAGAAAGGCGATTATACTTCAATCATTCATGGTAAATATTCTCATGAAGAAACTGTAGCGACAGCATCCTTTGCAGGAAAATATATAATCGTAAAGAACATGACAGAGGTATTCCCGTTGCATTTTTCCTTGTTGTCCAGTACAGCAGTTCTGCACTTTGCTGGATGTGGAATAATGTGTATTTTCTTAATGAACATTTGCCTTTATTTAGGCAACATATGTGTGTGACTATATCCTTGGAGGCGAACTTGATGGATCCAGCTCAACCAAAGAAGAGTTTATGAAGGTATGGTTCTCATTGCTTTCAACCATAATATATTGGCATCATTCACATCTTAAATTGTAATAATTTCTGGTCTGCAGAAATTCAAAAATGCAGTCTCAAAAGGGTTTGACCCAGAAGTTGACCTCACTAAAGTTGGCATTGCAAACCAGACTACAATGCTCAAGGGAGAAACAGAAGAGATCGGTAAAGATTTGATGAAATGCCATTTATTGCTTTCCACAGAAATTTCAAACTTGTTTTTCTTAAATTTCATTTCTAATTATCAGGAAAATTAGTCGAGAGAACTATGATGCGCAAGTACGGAGTGGGAAGTGTTAACGAGCACTTCATCAGTTTCAATACAATATGCGATGCTACTCAAGTAAGTCGGTCTCATGGATGTGCTTGTGGTGTATAGCATAAGGATTTTAGGGAGAAAAGTTAGGTTTAGTATTCTACATTCTCTTATGGCATGTTGTGTGCATGCATTAAGGGAATTCGTAACATTTTCCAAAATGGTTATTGAGAAAAGGAGGTATCCTCACTTGGGGAATAATCAATTAAACTGTCTGCTCCTGTAGAGATCCTACACTGGTTATCTTGCATACTTTTAAGTTGTCATCGACGTGTATCTAATCTTTTCTTCCCATCTATCAGGAGCGTCAAGATGCAATGTACAAGCTGGTGGAGGGAAAACTGGATCTCATGTTAGTTGTTGGTGGATGGAATTCCAGTAATACTTCTCATCTTCAAGAGATAGCCGAGGAGCGAGGAATTCCATCTTATTGGATTGATAGCGAGCAGAGAATAGGCCCAGGAAACAGAATAGCCTATAAACTGAATGTAAGTTACCATACAGATTTCAGCTCTAGTCCCTTTTGGTTTCAATTTCCCAACATTTTGTTGGTTTACCTTCCAATTTGCCATCACAATGTAAAGCCTACCTTTTCTTTCCTTGTTTACTTATAATTTTTTCGTCCTTGCAAATTTATGTTAGCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCCTATCACGATCGGTGTCACATCCGGTGCCTCAACCCCCGACAAGGTAAACCTCACTCTTCGACTTACATGATTGCTAGTAAAAATTGTTATCTGGAATGAATTTATAACCATACCATCTCTGTATGCGTTTTGAACAGGCTGTCGAAGATGCTCTTATCAAAGTCTTCGACATTAAGCGCGAAGAAGCATTGCAGTTTGCATAAATTCATAGGTTAGTAGTTCAAATTGACTTGTTAGGGACCAGAGTTGTGATGATTGTGAATGAATGTGGAACTGAAAGGCAGTACCCTCCCTTTTATCAATCATATAGTGTCTTGTATCGGTATATTAATGATGTGCAGTAAAGATCCTAGTCTTCACAGAAACGCGTCGTGTAGTTTGGGTAGTTCATCGAGTATGTCGACCTCCGACGCAGTTGAATCGTACCTTTAATTGTATCTTGTACACTTTTTCACCCTCTGGCTACAGAGAAAATATGATTGAATCAGAGTGTTGGGTTGCCTGTTATTTATTG

mRNA sequence

AGAAAAATGGCGTTACGAAACGAACCATAGAAAAAGCAAGACCTTTTTCGTAATTTTAAATGCGATCCCGGAATTAGGATCCTCCACTGATTCTACTTGACTAAACGCCAAAACTACGAATAAAAATAATAATAATAAATTTTTAGTGAACCATAAAATAAATCATAATAATATTAACAATAATAATTTCCACTGTAATTTTATCAATTTATATATAAAAAAAAAAAGAAATAGTTGATTCAAAGATAAATGGAGGGAGAAGAAGCAGAGGTCGTTTGTGAGTGGTAATTGAGCTCAAAACTAGTCTCTCCCATGGCGTTCTCTCTCCAACTTTGTCGTCTTCCTATTCGTACGGATGCGGCCTTGCCGGAGAATTTCGCCGGGATTCGAGCACTCCGGTACAGGAGGCCTCTCTCCGTGAGATGCGATGCTGATGCTTCTTCTTCTTCGCCTTCGGTGGCTGTGGATTCTGATTTTGACGCCAAGGTGTTCCGGAAGAATTTGACGAGGAGCGACAATTACAACCGCAAAGGGTTTGGTCACAAGGAGGAGACTCTGGCGCTCATGGATCGCGAGTATACCAGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTGAAACTGGCGGAAGCGTATGGATTCTGTTGGGGAGTTGAGCGGGCTGTTCAGATTGCCTATGAAGCGAGAAAGCAATTTCCAGAAGAGAAAATATGGATAACCAATGAAATTATTCACAACCCAACTGTTAACAAGCGCTTAGAGGATATGAAAGTTGAAAACATTCCACTCGAGGAAGGAAAGAAACAGTTTGAAGTGGTTGACAAGGGTGATGTTGTGATTTTGCCTGCATTTGGAGCTGCAGTGGACGAGATGTTGACATTGAGTGAAAAAAGTGTGCAAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTTTGGAATGTTGTTGAAAAGCACAAGAAAGGCGATTATACTTCAATCATTCATGGTAAATATTCTCATGAAGAAACTGTAGCGACAGCATCCTTTGCAGGAAAATATATAATCGTAAAGAACATGACAGAGGCAACATATGTGTGTGACTATATCCTTGGAGGCGAACTTGATGGATCCAGCTCAACCAAAGAAGAGTTTATGAAGAAATTCAAAAATGCAGTCTCAAAAGGGTTTGACCCAGAAGTTGACCTCACTAAAGTTGGCATTGCAAACCAGACTACAATGCTCAAGGGAGAAACAGAAGAGATCGGAAAATTAGTCGAGAGAACTATGATGCGCAAGTACGGAGTGGGAAGTGTTAACGAGCACTTCATCAGTTTCAATACAATATGCGATGCTACTCAAGAGCGTCAAGATGCAATGTACAAGCTGGTGGAGGGAAAACTGGATCTCATGTTAGTTGTTGGTGGATGGAATTCCAGTAATACTTCTCATCTTCAAGAGATAGCCGAGGAGCGAGGAATTCCATCTTATTGGATTGATAGCGAGCAGAGAATAGGCCCAGGAAACAGAATAGCCTATAAACTGAATCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCCTATCACGATCGGTGTCACATCCGGTGCCTCAACCCCCGACAAGGCTGTCGAAGATGCTCTTATCAAAGTCTTCGACATTAAGCGCGAAGAAGCATTGCAGTTTGCATAAATTCATAGGTTAGTAGTTCAAATTGACTTGTTAGGGACCAGAGTTGTGATGATTGTGAATGAATGTGGAACTGAAAGGCAGTACCCTCCCTTTTATCAATCATATAGTGTCTTGTATCGGTATATTAATGATGTGCAGTAAAGATCCTAGTCTTCACAGAAACGCGTCGTGTAGTTTGGGTAGTTCATCGAGTATGTCGACCTCCGACGCAGTTGAATCGTACCTTTAATTGTATCTTGTACACTTTTTCACCCTCTGGCTACAGAGAAAATATGATTGAATCAGAGTGTTGGGTTGCCTGTTATTTATTG

Coding sequence (CDS)

ATGGCGTTCTCTCTCCAACTTTGTCGTCTTCCTATTCGTACGGATGCGGCCTTGCCGGAGAATTTCGCCGGGATTCGAGCACTCCGGTACAGGAGGCCTCTCTCCGTGAGATGCGATGCTGATGCTTCTTCTTCTTCGCCTTCGGTGGCTGTGGATTCTGATTTTGACGCCAAGGTGTTCCGGAAGAATTTGACGAGGAGCGACAATTACAACCGCAAAGGGTTTGGTCACAAGGAGGAGACTCTGGCGCTCATGGATCGCGAGTATACCAGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTGAAACTGGCGGAAGCGTATGGATTCTGTTGGGGAGTTGAGCGGGCTGTTCAGATTGCCTATGAAGCGAGAAAGCAATTTCCAGAAGAGAAAATATGGATAACCAATGAAATTATTCACAACCCAACTGTTAACAAGCGCTTAGAGGATATGAAAGTTGAAAACATTCCACTCGAGGAAGGAAAGAAACAGTTTGAAGTGGTTGACAAGGGTGATGTTGTGATTTTGCCTGCATTTGGAGCTGCAGTGGACGAGATGTTGACATTGAGTGAAAAAAGTGTGCAAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTTTGGAATGTTGTTGAAAAGCACAAGAAAGGCGATTATACTTCAATCATTCATGGTAAATATTCTCATGAAGAAACTGTAGCGACAGCATCCTTTGCAGGAAAATATATAATCGTAAAGAACATGACAGAGGCAACATATGTGTGTGACTATATCCTTGGAGGCGAACTTGATGGATCCAGCTCAACCAAAGAAGAGTTTATGAAGAAATTCAAAAATGCAGTCTCAAAAGGGTTTGACCCAGAAGTTGACCTCACTAAAGTTGGCATTGCAAACCAGACTACAATGCTCAAGGGAGAAACAGAAGAGATCGGAAAATTAGTCGAGAGAACTATGATGCGCAAGTACGGAGTGGGAAGTGTTAACGAGCACTTCATCAGTTTCAATACAATATGCGATGCTACTCAAGAGCGTCAAGATGCAATGTACAAGCTGGTGGAGGGAAAACTGGATCTCATGTTAGTTGTTGGTGGATGGAATTCCAGTAATACTTCTCATCTTCAAGAGATAGCCGAGGAGCGAGGAATTCCATCTTATTGGATTGATAGCGAGCAGAGAATAGGCCCAGGAAACAGAATAGCCTATAAACTGAATCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCCTATCACGATCGGTGTCACATCCGGTGCCTCAACCCCCGACAAGGCTGTCGAAGATGCTCTTATCAAAGTCTTCGACATTAAGCGCGAAGAAGCATTGCAGTTTGCATAA

Protein sequence

MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA
Homology
BLAST of MC04g0500 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 1.1e-219
Identity = 373/466 (80.04%), Postives = 421/466 (90.34%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPEN-FAGIRALRYRRPLSVRCDA-DASSSSPSVAVDSDFDAK 60
           MA +LQ  RL +R D  + EN  +G  +LR R+ LSVRC + D ++ SPSV +DSDFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  VFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYG 120
           VFRKNLTRSDNYNRKGFGHKEETL LM+REYTSDI++TLK NG+ Y+WGDVTVKLA+AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVV 180
           FCWGVERAVQIAYEARKQFPEE++WITNEIIHNPTVNKRLEDM V+ IP+E+ KKQF+VV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 DKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYS 240
           +K DVVILPAFGA VDEM  L++K VQIVDTTCPWV+KVWN VEKHKKG+YTS+IHGKY+
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPE 300
           HEET+ATASFAGKYIIVKNM EA YVCDYILGG+ DGSSSTKEEFM+KFK A+SKGFDP+
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 VDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAM 360
            DL KVGIANQTTMLKGETEEIG+L+E TMMRKYGV +V+ HFISFNTICDATQERQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420
           Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN+IAYKL++GEL
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 VEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 465
           VEKEN+LPKGPITIGVTSGASTPDK VEDAL+KVFDIKREE LQ A
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

BLAST of MC04g0500 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 2.0e-213
Identity = 358/435 (82.30%), Postives = 403/435 (92.64%), Query Frame = 0

Query: 31  RRPLSVRCDADASSS-SPSVAVDSDFDAKVFRKNLTRSDNYNRKGFGHKEETLALMDREY 90
           R P SVRCD+ A+SS S S ++D+DFD K FR NLTRSDNYNRKGFGHK+ETL LM +EY
Sbjct: 25  RAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMSQEY 84

Query: 91  TSDIIKTLKENGFEYTWGDVTVKLAEAYGFCWGVERAVQIAYEARKQFPEEKIWITNEII 150
           TSD+IKTLKENG ++TWG VTVKLAEAYGFCWGVERAVQIAYEARKQFP+++IW+TNEII
Sbjct: 85  TSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTNEII 144

Query: 151 HNPTVNKRLEDMKVENIPLEEGKKQFEVVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDT 210
           HNPTVNKRLEDM V+NIP++ G K F+VV++GDVV+LPAFGAAV+EM TL+EK VQIVDT
Sbjct: 145 HNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQIVDT 204

Query: 211 TCPWVSKVWNVVEKHKKGDYTSIIHGKYSHEETVATASFAGKYIIVKNMTEATYVCDYIL 270
           TCPWVSKVWN+VEKHKKGDYTSIIHGKYSHEETVATASFAG YIIVKN+ EA+YVCDYIL
Sbjct: 205 TCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTYIIVKNIAEASYVCDYIL 264

Query: 271 GGELDGSSSTKEEFMKKFKNAVSKGFDPEVDLTKVGIANQTTMLKGETEEIGKLVERTMM 330
           GG+LDGSSSTKEEF++KFKNAVS GFDP+VDL KVGIANQTTMLKGETEEIGKLVE+TMM
Sbjct: 265 GGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTMLKGETEEIGKLVEKTMM 324

Query: 331 RKYGVGSVNEHFISFNTICDATQERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE 390
           R++GV +VN+HFI+FNTICDATQERQDAMY+LV+ K+DL+LVVGGWNSSNTSHLQEI E 
Sbjct: 325 RRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVVGGWNSSNTSHLQEIGEL 384

Query: 391 RGIPSYWIDSEQRIGPGNRIAYKLNHGELVEKENWLPKGPITIGVTSGASTPDKAVEDAL 450
            GIPSYWIDSEQRIGPGN+I+YKLNHGELVEKENWLP+GPITIGVTSGASTPDK VEDAL
Sbjct: 385 SGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITIGVTSGASTPDKVVEDAL 444

Query: 451 IKVFDIKREEALQFA 465
            KVF+IKR+E LQ A
Sbjct: 445 QKVFEIKRQEVLQAA 459

BLAST of MC04g0500 vs. ExPASy Swiss-Prot
Match: B0C4N8 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=ispH PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 5.7e-152
Identity = 258/408 (63.24%), Postives = 318/408 (77.94%), Query Frame = 0

Query: 56  DAKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAE 115
           D K F+++L +S+ Y+RKGFGH+EE   L+  EY S +I+ ++ NG+    GDVT+ LAE
Sbjct: 2   DTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLAE 61

Query: 116 AYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLE-EGKKQ 175
           A+GFCWGVERA+ +AYE R+QFP+E+IWITNEIIHNP+VNK L DMKVE IP++ +G+K 
Sbjct: 62  AFGFCWGVERAIALAYETRRQFPQERIWITNEIIHNPSVNKNLRDMKVEFIPVQPDGQKD 121

Query: 176 FEVVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIH 235
           F VV + DVVILPAFGA+V EM  L +K   I+DTTCPWVSKVW  VEKHKKG YTSIIH
Sbjct: 122 FTVVGQNDVVILPAFGASVQEMQLLHDKGCTIMDTTCPWVSKVWTSVEKHKKGSYTSIIH 181

Query: 236 GKYSHEETVATASFAGKYIIVKNMTEATYVCDYIL--GGELDGSSSTKEEFMKKFKNAVS 295
           GKY HEET+AT+SFAG Y+++ N+ EA YV DYIL  G         + +FM KF NA S
Sbjct: 182 GKYKHEETIATSSFAGTYLVLLNLEEAEYVADYILNPGDTPSEREQRRADFMAKFANAHS 241

Query: 296 KGFDPEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQ 355
            GFDPE DL +VGIANQTTMLKGETE+IGKL ERT+MRKYG   VNEHF+SFNTICDATQ
Sbjct: 242 PGFDPETDLERVGIANQTTMLKGETEQIGKLFERTVMRKYGPAQVNEHFLSFNTICDATQ 301

Query: 356 ERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYK 415
           ERQDA+++LV+  LDLM+V+GG+NSSNT+HLQEI+ ++ IPSY IDS  RIGPGNR+ +K
Sbjct: 302 ERQDAIFQLVDEPLDLMVVIGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEHK 361

Query: 416 LNHGELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEA 461
             + +L   +NWLP GP+ IGVTSGASTPDK V D + K+F IK   A
Sbjct: 362 PLNADLTVTDNWLPNGPLAIGVTSGASTPDKVVSDIVEKIFSIKSASA 409

BLAST of MC04g0500 vs. ExPASy Swiss-Prot
Match: Q31S64 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=ispH PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 3.7e-151
Identity = 252/402 (62.69%), Postives = 319/402 (79.35%), Query Frame = 0

Query: 56  DAKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAE 115
           D K F++ L +SD YNRKGFG   +    ++  Y SD+I++L++NG+    G++T++LAE
Sbjct: 2   DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAE 61

Query: 116 AYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQF 175
           A+GFCWGVERAV IAYE R+ FP+E+IWITNEIIHNP+VN+ L +M VE IP E G+K F
Sbjct: 62  AFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDF 121

Query: 176 EVVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHG 235
            VVD+GDVVILPAFGA+V EM  L EK   IVDTTCPWVSKVWN VEKHK+G +TSIIHG
Sbjct: 122 SVVDRGDVVILPAFGASVQEMQLLDEKGCHIVDTTCPWVSKVWNTVEKHKRGAHTSIIHG 181

Query: 236 KYSHEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGF 295
           KY+HEETVAT+SFA  Y++V N+ +A YVCDYIL G        ++EFM +F  A S GF
Sbjct: 182 KYNHEETVATSSFAETYLVVLNLEQAQYVCDYILNG------GDRDEFMTRFGKACSAGF 241

Query: 296 DPEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQ 355
           DP+ DL ++GIANQTTMLK ETE IGKL ERT+++KYG  ++N+HF++FNTICDATQERQ
Sbjct: 242 DPDRDLERIGIANQTTMLKSETEAIGKLFERTLLKKYGPQALNDHFLAFNTICDATQERQ 301

Query: 356 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 415
           DAM++LVE  LDL++V+GG+NSSNT+HLQEIA ER IPS+ ID+ +RIGPGNRI +K  H
Sbjct: 302 DAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLH 361

Query: 416 GELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKR 458
            +L   E WLP GP+TIG+TSGASTPDK VED + ++FD++R
Sbjct: 362 TDLTTTEPWLPAGPLTIGITSGASTPDKVVEDVIERLFDLQR 397

BLAST of MC04g0500 vs. ExPASy Swiss-Prot
Match: Q5N249 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=ispH PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 3.7e-151
Identity = 252/402 (62.69%), Postives = 319/402 (79.35%), Query Frame = 0

Query: 56  DAKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAE 115
           D K F++ L +SD YNRKGFG   +    ++  Y SD+I++L++NG+    G++T++LAE
Sbjct: 2   DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAE 61

Query: 116 AYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQF 175
           A+GFCWGVERAV IAYE R+ FP+E+IWITNEIIHNP+VN+ L +M VE IP E G+K F
Sbjct: 62  AFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDF 121

Query: 176 EVVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHG 235
            VVD+GDVVILPAFGA+V EM  L EK   IVDTTCPWVSKVWN VEKHK+G +TSIIHG
Sbjct: 122 SVVDRGDVVILPAFGASVQEMQLLDEKGCHIVDTTCPWVSKVWNTVEKHKRGAHTSIIHG 181

Query: 236 KYSHEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGF 295
           KY+HEETVAT+SFA  Y++V N+ +A YVCDYIL G        ++EFM +F  A S GF
Sbjct: 182 KYNHEETVATSSFAETYLVVLNLEQAQYVCDYILNG------GDRDEFMTRFGKACSAGF 241

Query: 296 DPEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQ 355
           DP+ DL ++GIANQTTMLK ETE IGKL ERT+++KYG  ++N+HF++FNTICDATQERQ
Sbjct: 242 DPDRDLERIGIANQTTMLKSETEAIGKLFERTLLKKYGPQALNDHFLAFNTICDATQERQ 301

Query: 356 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 415
           DAM++LVE  LDL++V+GG+NSSNT+HLQEIA ER IPS+ ID+ +RIGPGNRI +K  H
Sbjct: 302 DAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLH 361

Query: 416 GELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKR 458
            +L   E WLP GP+TIG+TSGASTPDK VED + ++FD++R
Sbjct: 362 TDLTTTEPWLPAGPLTIGITSGASTPDKVVEDVIERLFDLQR 397

BLAST of MC04g0500 vs. NCBI nr
Match: XP_022136222.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Momordica charantia])

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60
           MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF
Sbjct: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60

Query: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120
           RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC
Sbjct: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240
           GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE
Sbjct: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240

Query: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300
           ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD
Sbjct: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300

Query: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360
           LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK
Sbjct: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360

Query: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420
           LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE
Sbjct: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420

Query: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA
Sbjct: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464

BLAST of MC04g0500 vs. NCBI nr
Match: XP_008454877.1 (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Cucumis melo] >KAA0031252.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Cucumis melo var. makuwa] >TYK06703.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 867 bits (2239), Expect = 0.0
Identity = 425/464 (91.59%), Postives = 450/464 (96.98%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60
           MAFS+QL RLP RTDAALPEN + +R+LRYR+P+SVRCD D+SSSS S AVDSDFDAKVF
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGDSSSSS-SAAVDSDFDAKVF 60

Query: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120
           R+NLT+SDNYNRKGFG+K+ETLALM+REYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC
Sbjct: 61  RRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVNKRLE+M+V+NIPLEEG+KQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240
           GDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGDYTSIIHGKY+HE
Sbjct: 181 GDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300
           ETVATASFAGKYIIVKNM EA YVCDYILGGELDGSSSTKEEFMKKFKNAVS+GFDP+VD
Sbjct: 241 ETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDVD 300

Query: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360
           L KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHF+SFNTICDATQERQDAMYK
Sbjct: 301 LLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYK 360

Query: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420
           LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE
Sbjct: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420

Query: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           KENWLPKG ITIGVTSGASTPDK VEDALIKVFDIKREEALQFA
Sbjct: 421 KENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463

BLAST of MC04g0500 vs. NCBI nr
Match: AEM42976.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Siraitia grosvenorii])

HSP 1 Score: 862 bits (2228), Expect = 1.47e-314
Identity = 424/470 (90.21%), Postives = 452/470 (96.17%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPS------VAVDSD 60
           MAFSLQL RL  RTDAALPENF+GIRA+RYR+PLSVRCD D+SSSS S      VA+DS+
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLA 120
           FDAKVFRKNLTRS+NYNRKGFG+K+ETLALM+REYTSDIIKTLKE+ +EYTWGDVTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQ 180
           EAYGFCWGVERAVQIAYEARKQFP+EKIWITNEIIHNPTVNKRLE+M+VENIPLEEGKKQ
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVENIPLEEGKKQ 180

Query: 181 FEVVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIH 240
           FEVV+KGDVVILPAFGAAVDEMLTLSEK+VQIVDTTCPWVSKVWNVVEKHKKGD+TSIIH
Sbjct: 181 FEVVNKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDFTSIIH 240

Query: 241 GKYSHEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKG 300
           GKY+HEETVATASFAGKYIIVKNM EATYVCDYILGGELDGSS TKEEFMKKFKNAVSKG
Sbjct: 241 GKYAHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSPTKEEFMKKFKNAVSKG 300

Query: 301 FDPEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQER 360
           FDP++DL KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHFISFNTICDATQER
Sbjct: 301 FDPDIDLVKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFISFNTICDATQER 360

Query: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLN 420
           QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQ+IAE+RGIPSYW+DSEQRIGPGN+IAYKLN
Sbjct: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQQIAEDRGIPSYWVDSEQRIGPGNKIAYKLN 420

Query: 421 HGELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           HGELVEKENWLP+GPITIGVTSGASTPDK VED LIKVFDIKREEALQFA
Sbjct: 421 HGELVEKENWLPRGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQFA 470

BLAST of MC04g0500 vs. NCBI nr
Match: XP_004137008.2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus])

HSP 1 Score: 862 bits (2226), Expect = 2.95e-314
Identity = 422/468 (90.17%), Postives = 449/468 (95.94%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPS----VAVDSDFD 60
           MAFS+QL RLP RTDAA+PENF  +R+LRYR+P+SVRCD D+SSSS S     AVDS+FD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFR+NLT+SDNYNRKGFG+K+ETLALM+REYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVNKRLE+M+V+NIPLE+G+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGDYTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YSHEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           Y+HEETVATASFAG +IIVKNM EA YVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQD 360
           P+VDL KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHF+SFNTICDATQERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           ELVEKENWLPKG ITIGVTSGASTPDK VEDALIKVFDIKREEALQFA
Sbjct: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 468

BLAST of MC04g0500 vs. NCBI nr
Match: XP_038888882.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Benincasa hispida])

HSP 1 Score: 860 bits (2222), Expect = 9.00e-314
Identity = 419/464 (90.30%), Postives = 450/464 (96.98%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60
           MAFS+QL RLP RTDA LPENFA +R++RYR+PLSVRCD D+SSS+   AVDSDFDAKVF
Sbjct: 1   MAFSMQLYRLPTRTDALLPENFATLRSVRYRKPLSVRCDGDSSSSA---AVDSDFDAKVF 60

Query: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120
           R+NLT+S+NYNR+GFG+K+ETLALM+REYTSD+IKTLKENGFEYTWG+VTVKLAEAYGFC
Sbjct: 61  RRNLTKSENYNRRGFGYKDETLALMNREYTSDVIKTLKENGFEYTWGNVTVKLAEAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVNKRLE+M+V+NIPLEEG+KQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240
           GDVVILPAFGAAV+EMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGDYTSIIHGKY+HE
Sbjct: 181 GDVVILPAFGAAVEEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300
           ETVATASFAGKYIIVKNM EA YVCDYILGGELDGSSSTKEEFMKKFKNAVS+GFDP++D
Sbjct: 241 ETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDID 300

Query: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360
           L KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHF+SFNTICDATQERQDAMYK
Sbjct: 301 LLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYK 360

Query: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420
           LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE
Sbjct: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420

Query: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           KENWLPKGPITIGVTSGASTPDK VEDALIKVFDIKREEALQFA
Sbjct: 421 KENWLPKGPITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 461

BLAST of MC04g0500 vs. ExPASy TrEMBL
Match: A0A6J1C4Z2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007971 PE=3 SV=1)

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60
           MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF
Sbjct: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60

Query: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120
           RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC
Sbjct: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240
           GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE
Sbjct: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240

Query: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300
           ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD
Sbjct: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300

Query: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360
           LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK
Sbjct: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360

Query: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420
           LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE
Sbjct: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420

Query: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA
Sbjct: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464

BLAST of MC04g0500 vs. ExPASy TrEMBL
Match: A0A5A7SJM1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00040 PE=3 SV=1)

HSP 1 Score: 867 bits (2239), Expect = 0.0
Identity = 425/464 (91.59%), Postives = 450/464 (96.98%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60
           MAFS+QL RLP RTDAALPEN + +R+LRYR+P+SVRCD D+SSSS S AVDSDFDAKVF
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGDSSSSS-SAAVDSDFDAKVF 60

Query: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120
           R+NLT+SDNYNRKGFG+K+ETLALM+REYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC
Sbjct: 61  RRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVNKRLE+M+V+NIPLEEG+KQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240
           GDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGDYTSIIHGKY+HE
Sbjct: 181 GDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300
           ETVATASFAGKYIIVKNM EA YVCDYILGGELDGSSSTKEEFMKKFKNAVS+GFDP+VD
Sbjct: 241 ETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDVD 300

Query: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360
           L KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHF+SFNTICDATQERQDAMYK
Sbjct: 301 LLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYK 360

Query: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420
           LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE
Sbjct: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420

Query: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           KENWLPKG ITIGVTSGASTPDK VEDALIKVFDIKREEALQFA
Sbjct: 421 KENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463

BLAST of MC04g0500 vs. ExPASy TrEMBL
Match: A0A1S3BZP5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103495189 PE=3 SV=1)

HSP 1 Score: 867 bits (2239), Expect = 0.0
Identity = 425/464 (91.59%), Postives = 450/464 (96.98%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPSVAVDSDFDAKVF 60
           MAFS+QL RLP RTDAALPEN + +R+LRYR+P+SVRCD D+SSSS S AVDSDFDAKVF
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGDSSSSS-SAAVDSDFDAKVF 60

Query: 61  RKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120
           R+NLT+SDNYNRKGFG+K+ETLALM+REYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC
Sbjct: 61  RRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVNKRLE+M+V+NIPLEEG+KQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYSHE 240
           GDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGDYTSIIHGKY+HE
Sbjct: 181 GDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPEVD 300
           ETVATASFAGKYIIVKNM EA YVCDYILGGELDGSSSTKEEFMKKFKNAVS+GFDP+VD
Sbjct: 241 ETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDVD 300

Query: 301 LTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAMYK 360
           L KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHF+SFNTICDATQERQDAMYK
Sbjct: 301 LLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYK 360

Query: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420
           LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE
Sbjct: 361 LVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVE 420

Query: 421 KENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           KENWLPKG ITIGVTSGASTPDK VEDALIKVFDIKREEALQFA
Sbjct: 421 KENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463

BLAST of MC04g0500 vs. ExPASy TrEMBL
Match: K7NBR0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 862 bits (2228), Expect = 7.12e-315
Identity = 424/470 (90.21%), Postives = 452/470 (96.17%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPS------VAVDSD 60
           MAFSLQL RL  RTDAALPENF+GIRA+RYR+PLSVRCD D+SSSS S      VA+DS+
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLA 120
           FDAKVFRKNLTRS+NYNRKGFG+K+ETLALM+REYTSDIIKTLKE+ +EYTWGDVTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQ 180
           EAYGFCWGVERAVQIAYEARKQFP+EKIWITNEIIHNPTVNKRLE+M+VENIPLEEGKKQ
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVENIPLEEGKKQ 180

Query: 181 FEVVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIH 240
           FEVV+KGDVVILPAFGAAVDEMLTLSEK+VQIVDTTCPWVSKVWNVVEKHKKGD+TSIIH
Sbjct: 181 FEVVNKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDFTSIIH 240

Query: 241 GKYSHEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKG 300
           GKY+HEETVATASFAGKYIIVKNM EATYVCDYILGGELDGSS TKEEFMKKFKNAVSKG
Sbjct: 241 GKYAHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSPTKEEFMKKFKNAVSKG 300

Query: 301 FDPEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQER 360
           FDP++DL KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHFISFNTICDATQER
Sbjct: 301 FDPDIDLVKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFISFNTICDATQER 360

Query: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLN 420
           QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQ+IAE+RGIPSYW+DSEQRIGPGN+IAYKLN
Sbjct: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQQIAEDRGIPSYWVDSEQRIGPGNKIAYKLN 420

Query: 421 HGELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           HGELVEKENWLP+GPITIGVTSGASTPDK VED LIKVFDIKREEALQFA
Sbjct: 421 HGELVEKENWLPRGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQFA 470

BLAST of MC04g0500 vs. ExPASy TrEMBL
Match: A0A0A0K3D8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G075680 PE=3 SV=1)

HSP 1 Score: 862 bits (2226), Expect = 1.43e-314
Identity = 422/468 (90.17%), Postives = 449/468 (95.94%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPENFAGIRALRYRRPLSVRCDADASSSSPS----VAVDSDFD 60
           MAFS+QL RLP RTDAA+PENF  +R+LRYR+P+SVRCD D+SSSS S     AVDS+FD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFR+NLT+SDNYNRKGFG+K+ETLALM+REYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVNKRLE+M+V+NIPLE+G+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGDYTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YSHEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300
           Y+HEETVATASFAG +IIVKNM EA YVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PEVDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQD 360
           P+VDL KVGIANQTTMLKGETEEIGKLVERTMMRK+GV +VNEHF+SFNTICDATQERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 464
           ELVEKENWLPKG ITIGVTSGASTPDK VEDALIKVFDIKREEALQFA
Sbjct: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 468

BLAST of MC04g0500 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 763.8 bits (1971), Expect = 7.8e-221
Identity = 373/466 (80.04%), Postives = 421/466 (90.34%), Query Frame = 0

Query: 1   MAFSLQLCRLPIRTDAALPEN-FAGIRALRYRRPLSVRCDA-DASSSSPSVAVDSDFDAK 60
           MA +LQ  RL +R D  + EN  +G  +LR R+ LSVRC + D ++ SPSV +DSDFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  VFRKNLTRSDNYNRKGFGHKEETLALMDREYTSDIIKTLKENGFEYTWGDVTVKLAEAYG 120
           VFRKNLTRSDNYNRKGFGHKEETL LM+REYTSDI++TLK NG+ Y+WGDVTVKLA+AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEDMKVENIPLEEGKKQFEVV 180
           FCWGVERAVQIAYEARKQFPEE++WITNEIIHNPTVNKRLEDM V+ IP+E+ KKQF+VV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 DKGDVVILPAFGAAVDEMLTLSEKSVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYS 240
           +K DVVILPAFGA VDEM  L++K VQIVDTTCPWV+KVWN VEKHKKG+YTS+IHGKY+
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGKYIIVKNMTEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFDPE 300
           HEET+ATASFAGKYIIVKNM EA YVCDYILGG+ DGSSSTKEEFM+KFK A+SKGFDP+
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 VDLTKVGIANQTTMLKGETEEIGKLVERTMMRKYGVGSVNEHFISFNTICDATQERQDAM 360
            DL KVGIANQTTMLKGETEEIG+L+E TMMRKYGV +V+ HFISFNTICDATQERQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420
           Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN+IAYKL++GEL
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 VEKENWLPKGPITIGVTSGASTPDKAVEDALIKVFDIKREEALQFA 465
           VEKEN+LPKGPITIGVTSGASTPDK VEDAL+KVFDIKREE LQ A
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B351.1e-21980.044-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
Q6AVG62.0e-21382.304-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
B0C4N85.7e-15263.244-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (stra... [more]
Q31S643.7e-15162.694-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (s... [more]
Q5N2493.7e-15162.694-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ... [more]
Match NameE-valueIdentityDescription
XP_022136222.10.0100.004-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Momordica cha... [more]
XP_008454877.10.091.59PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-lik... [more]
AEM42976.11.47e-31490.214-hydroxy-3-methylbut-2-enyl diphosphate reductase [Siraitia grosvenorii][more]
XP_004137008.22.95e-31490.174-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativ... [more]
XP_038888882.19.00e-31490.304-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Benincasa his... [more]
Match NameE-valueIdentityDescription
A0A6J1C4Z20.0100.004-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Momordica c... [more]
A0A5A7SJM10.091.594-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa O... [more]
A0A1S3BZP50.091.594-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Cucumi... [more]
K7NBR07.12e-31590.214-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii OX=19... [more]
A0A0A0K3D81.43e-31490.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G075680 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G34350.17.8e-22180.044-hydroxy-3-methylbut-2-enyl diphosphate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 111..448
e-value: 7.2E-83
score: 277.9
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseTIGRFAMTIGR00216TIGR00216coord: 111..449
e-value: 1.3E-89
score: 298.1
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseHAMAPMF_00191IspHcoord: 109..456
score: 28.543606
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseCDDcd13944lytB_ispHcoord: 111..449
e-value: 1.6127E-104
score: 310.496
NoneNo IPR availableGENE3D3.40.50.11270coord: 120..210
e-value: 5.1E-22
score: 79.7
NoneNo IPR availableGENE3D3.40.1010.20coord: 345..463
e-value: 1.4E-35
score: 123.4
NoneNo IPR availableGENE3D3.40.1010.20coord: 211..344
e-value: 5.5E-19
score: 70.0
NoneNo IPR availablePANTHERPTHR316194-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 1..462

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0500.1MC04g0500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding