MC04g0492 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0492
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NEDD8-conjugating enzyme Ubc12-like
Location	MC04: 4135731 .. 4139979 (-)
RNA-Seq Expression	MC04g0492
Synteny	MC04g0492

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATGAAAAGTATGGTGCGACTGCGGGTGCAGAAGAACAAACGTGAACCATGAAACTGGAGATAGCTTCCACACATCTCTATTCTCTGGTCCGGTGGTCCCGTAAACGGCGCAGTGTCGCAGTAAATCGACCGAGCTGTCACTCACCGTCCTTCGTGGCGCTCTCTGATTGGCCGTCCCGTCCGAACAAGCTTTGTAATAAGACGGATGACGCCGGTCCTCGGTCGAAACTCGAAAGCGAAGGGAGCCCGGCTTTGGCCTGCACAGCGTACGACGATAATTGACACAGACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGAGAGAGGGAGAGTGAGTTCCGAATCAGATCCAGCTCCCTCCTTCTTTCTCTCTCACTTTCTACACGCTTTCGGGCCTGCTTCAGCTCTATCCTCTCCAGACTCTGTGCTCGTTCAAATCCAGGTTGAAATCGAGGTAAGCATCCGCCTCCGACTCTCTGCGCTTTCGATTTCTTGTATTGCGATCGCGTCCGTTTTTCTTTTCTGGTTTATTTCGTTTTGTTTTACGCTTGATTGATCCAGGATTGCTGTTCATTCATTTTTCCGTTCGTTTTTTTATATGTAATCGATGAATCATGTCTTGATTGTGCGTGTTGCTGTGTCTTGGTGAGATCCTAATTGCCGTGGTTCTTTGATTTGAGATTCTATAATGTTCTGTGTGTATATTCGCGGTTTGTTTTAGTTGTTTTGATACCTCGAGTGTTTTGCATCTCGTCTACTTGCGGATGAGGAAGTTAACGCGTTTATCTTTTTTTTTGTTTTGTTTGTGTGTTTTGATACTTGAGTTTGCTGGCATACGGCACCAGATATCTGGTGTTTGCTATCATGATCAGGTATTCTGATCCAGTTGATGCTTGGCTTGATTAGATAGTTATGTGAGCGATGTATTGGTAATAGATGTGGTATGCTGCCTGGAAAATGCTAGGGAATTTTTCTCAGGAAGTTTGGGGTTTCCTTCGATTTTATTATAGCAGTTGTTAGTACGATTGTTTAGGAATGCCGATTTTGCTTCATCAATCATCCATGGTGTCATTATCATAACTGTAATAAATGTCGTTAGGCCGTAAGTCTGCTATTTCAAGTCACTGGTTCAAAAGTGCATTTTATGACCATCAGTGTTATGATTTGATTGGGCAGTTTAAGTCCTTGGTGTTTGAGGGTCTTGATATGTATTAGAATGCTAATACTGAGTTGGCCGAATCTATGATTCTCAACTTTTTCAAATTCTCGTGCTTCCTAAATTCAGATTAGAAGCTTTGTACTACTTAGATCTGGTAGTGCTGTAAAATTGATGTCCTATTTTTGAAACTGCAATGCAGGAACGATGATTAAGCTTTTTAAGGTGAAGGAAAAGCAGAGAGAGATTGCAGAGAATGCCAATGGGAAACCCCCTGTCAAGAAGCAAAGTGCAGGAGAGTTGCGTCTTCAAAAAGGTATTCTTTCACATTAATTTTAATTTTCTCTACTGTTGTGATTTTATTAGGTTTTTTGAGGTACCCTGAACGAAGGAAACAATCTCTCCCCTCTCCTAAAAGTTGATTGATTAGTCTCATTCCATATTGACTACTGTTGACCTTGAAGCCATGAGTTCTACGGTTTGTTTAGAACCATTTTCTGAAAATCCTATTGTTGTTGTATTTAATAGCATCCATTGTCTTGGCTCATGTTCAGATCAGATTTATGTTATGTATTTCTTAGTTTTAATTTTTAAATATGATATAGTGAGCAGGGCCGTGAGAACTTGAATCATCTTTTACTCTGTTGATTTTCCTCTTTTTGCTGGAATTTATTGTTAATCAGTCTAGAATTGGTTGTTTTAGCCAATGATTATAGAGAAATCCTTCAGCAGCTTTCGTGTGAACAGTATTACAAAGGCAGGGTTTGTATCCTGTGACATAATGCAGTCAAGACTCTTACATTTTACTTGACACTGGTTTATTACTAGCTTCATCTTGGTATACTCTCTCTAAAAGTCTTGCAATTTTTCTAGTTTACTTATTTATTTATTTTATTAATAAATTTAATATTAAGGCCAACTGGAACAGCTTCTTATTATCTATGTGGCTTTGGGTGGAAGATGTTTCTTTATCTTCTTTTTGTATTTTCCTTCCTCTGATGAGCGAAATTTATCTTATCCTACATGTGTGAATATGATTTAAACATTCATCAAAATTTCTATTCGCTGTGAAATGACATGGCTATCTACCAATGACATTGATGTTACAGCAACTTCTAGAAAAATATGTCTTCTGGCTATACATGCAGTTCAAGGATTTTTATTTTTAATTGTAGATATCAGTGAATTGAACCTACCGAAAACGTGTAGTATATCATTTCCTAATGGCAAGGATGACTTGATGAACTTTGAGGTCACTATTCATCCTGATGAAGGATATTATCTGTAAGTAGTCTCTTGAAAAGATTGGTTTTCAGTTTAGTTGCAATATATACTTCTTAAGTTGAGATGCATGTGTTAAAAGACATTACAATTCCCGGATCTCAGACATGCATCTTATGACTGAAACAATGGGAGCTGAAGATATATGCATGGATGTCATATTATTTCTCTCCATGAGGCCTCAATGGAACTTTGGATTGGACTTGGTTCAACTGTATTTAAGTTGTGTATTGAATCTTGCCTGCTTTTTCATGTTCTAATAATCATTTCTTGGTTAAGATGTCTGTTCCCCCTCCACCCGCTGCGGCCGTTCCTTGGTAATGAGCTTAAAAAGTCTCTTAATTTCATTTTAACAGAGGTGGTACGTTTCTATTCTCTTTCCAAGTTTCCCCTGTCTACCCTCACGAGCCACCAAAAGTGAAGTGCAAGACAAAGGTAATGATGCTGATCTGATTCATTGACTTTCGTTTTATTTATTTATTTATTATTGTTAATGTTTGGAATTAGCTTTAGATAACATATTTGACTTGCAGGTGTATCATCCTAATATTGACTTGGAAGGAAATGTCTGCCTCAATATTTTAAGAGAGGACTGGAAGCCCGTTCTGAATATCAACACCATAATATACGGATTATTTCATCTTTTCACGGTATTTATGATTGTGCCAATTATATACCCTTAAATTTTAGCCTTTAGGTTTAAAATCTATTTAGGTCCTGCATTTTCATACTTATTTGGTTATAGTTCCTAAGTTTTCAATTAGTTTTGTCACTCAACTTCCTAATGTTTTGTTCTAATTCCTAAACTTTGTATAAATGGTATTTTAGTTATGCCATCGATCTTTATTTCCACTAATCATTTCGAAAAAAATAATTGAACCTTGTTTGGAACTCAAAATCTGACCCTACTGCATGTGTAGCTTCAAATATTTGCAAATATCTACTCATCAAGTAACCAAAGACGCATAAATATACATCTATGTCAAGTTTCTATAAAATAATTTACAAAAGCAAAATTAACAATAAAGACAGTTTTTTTTATGTGAATTTCCGAGACCAGAATGAAGTTGGGACAAAAATGGAATACGCATAAAAATTTGAGGAACGATATAAGATTTAGACCTAACGTTCACCAACTTGTGGGGGGTTTTCTTTAATTACAATATTTTTCCCATCATTCTTGCAGCAACCAAATCATGAAGATCCCCTCAATCATGATGCCGCTGCTGTTTTGAGGGATAACCCCAAGTTGTTCGAATCCAATGTGAGAAGAGCCATGGCTGGGGGCTACGTTGGGCAGACTTTCTTCACACGTTGCATGTAGATTCTACATGAATTTGTAAAGCACCAGAAGATACATCATTTGAAGCATGGATTTGGCGAGGTCTTCGAGCCTTTCTGGTAAGGTAACGTGTTTAACAGGACTATCTCATCATCGACACGACGTGATGTGCTTTGTGATGCTGGTGGAACTTTGTTGTGTGTTTGCAGTATTGGTTTCAAATTCTCTCAAAGTGTATTGCTGGAAGTTGGACTGGAAACTGAACCTCTTATCTGTGTATGTATATCTTCTAATTAATTTGTCTTGAAAATGACAGATTGCTCTTTTCCTATCAGTTCAAAAGTGCAAGGTATGTTCTTGGAGCCTCATCATCTCTTTTCTTTCTGAGTCTCTACTACTATGGATGTAAATTTACCAAATTGAATCACATAAGATTTTATACATCTCAAGTTTGTAAAATCTCTGTTAGTTTCTCATTTTGTCAGCATCATGAGATGGGAAAGTTTTTCATTTTTAACTTCTCT

mRNA sequence

AAAAATGAAAAGTATGGTGCGACTGCGGGTGCAGAAGAACAAACGTGAACCATGAAACTGGAGATAGCTTCCACACATCTCTATTCTCTGGTCCGGTGGTCCCGTAAACGGCGCAGTGTCGCAGTAAATCGACCGAGCTGTCACTCACCGTCCTTCGTGGCGCTCTCTGATTGGCCGTCCCGTCCGAACAAGCTTTGTAATAAGACGGATGACGCCGGTCCTCGGTCGAAACTCGAAAGCGAAGGGAGCCCGGCTTTGGCCTGCACAGCGTACGACGATAATTGACACAGACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGAGAGAGGGAGAGTGAGTTCCGAATCAGATCCAGCTCCCTCCTTCTTTCTCTCTCACTTTCTACACGCTTTCGGGCCTGCTTCAGCTCTATCCTCTCCAGACTCTGTGCTCGTTCAAATCCAGGTTGAAATCGAGGAACGATGATTAAGCTTTTTAAGGTGAAGGAAAAGCAGAGAGAGATTGCAGAGAATGCCAATGGGAAACCCCCTGTCAAGAAGCAAAGTGCAGGAGAGTTGCGTCTTCAAAAAGATATCAGTGAATTGAACCTACCGAAAACGTGTAGTATATCATTTCCTAATGGCAAGGATGACTTGATGAACTTTGAGGTCACTATTCATCCTGATGAAGGATATTATCTAGGTGGTACGTTTCTATTCTCTTTCCAAGTTTCCCCTGTCTACCCTCACGAGCCACCAAAAGTGAAGTGCAAGACAAAGGTGTATCATCCTAATATTGACTTGGAAGGAAATGTCTGCCTCAATATTTTAAGAGAGGACTGGAAGCCCGTTCTGAATATCAACACCATAATATACGGATTATTTCATCTTTTCACGCAACCAAATCATGAAGATCCCCTCAATCATGATGCCGCTGCTGTTTTGAGGGATAACCCCAAGTTGTTCGAATCCAATGTGAGAAGAGCCATGGCTGGGGGCTACGTTGGGCAGACTTTCTTCACACGTTGCATGTAGATTCTACATGAATTTGTAAAGCACCAGAAGATACATCATTTGAAGCATGGATTTGGCGAGGTCTTCGAGCCTTTCTGGTAAGGTAACGTGTTTAACAGGACTATCTCATCATCGACACGACGTGATGTGCTTTGTGATGCTGGTGGAACTTTGTTGTGTGTTTGCAGTATTGGTTTCAAATTCTCTCAAAGTGTATTGCTGGAAGTTGGACTGGAAACTGAACCTCTTATCTGTGTATGTATATCTTCTAATTAATTTGTCTTGAAAATGACAGATTGCTCTTTTCCTATCAGTTCAAAAGTGCAAGGTATGTTCTTGGAGCCTCATCATCTCTTTTCTTTCTGAGTCTCTACTACTATGGATGTAAATTTACCAAATTGAATCACATAAGATTTTATACATCTCAAGTTTGTAAAATCTCTGTTAGTTTCTCATTTTGTCAGCATCATGAGATGGGAAAGTTTTTCATTTTTAACTTCTCT

Coding sequence (CDS)

ATGATTAAGCTTTTTAAGGTGAAGGAAAAGCAGAGAGAGATTGCAGAGAATGCCAATGGGAAACCCCCTGTCAAGAAGCAAAGTGCAGGAGAGTTGCGTCTTCAAAAAGATATCAGTGAATTGAACCTACCGAAAACGTGTAGTATATCATTTCCTAATGGCAAGGATGACTTGATGAACTTTGAGGTCACTATTCATCCTGATGAAGGATATTATCTAGGTGGTACGTTTCTATTCTCTTTCCAAGTTTCCCCTGTCTACCCTCACGAGCCACCAAAAGTGAAGTGCAAGACAAAGGTGTATCATCCTAATATTGACTTGGAAGGAAATGTCTGCCTCAATATTTTAAGAGAGGACTGGAAGCCCGTTCTGAATATCAACACCATAATATACGGATTATTTCATCTTTTCACGCAACCAAATCATGAAGATCCCCTCAATCATGATGCCGCTGCTGTTTTGAGGGATAACCCCAAGTTGTTCGAATCCAATGTGAGAAGAGCCATGGCTGGGGGCTACGTTGGGCAGACTTTCTTCACACGTTGCATGTAG

Protein sequence

MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFTRCM

Homology

[Close]

BLAST of MC04g0492 vs. ExPASy Swiss-Prot
Match: Q9SDY5 (NEDD8-conjugating enzyme Ubc12 OS=Arabidopsis thaliana OX=3702 GN=RCE1 PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 2.1e-89
Identity = 158/184 (85.87%), Postives = 168/184 (91.30%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENA-NGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLM 60
           MI LFKVKEKQRE A+NA  G   VKKQSAGELRL KDISELNLP +CSISFPNGKDDLM
Sbjct: 1   MIGLFKVKEKQREQAQNATRGGASVKKQSAGELRLHKDISELNLPSSCSISFPNGKDDLM 60

Query: 61  NFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRED 120
           NFEV+I PD+GYY  GTF+F+FQVSPVYPHE PKVKCKTKVYHPNIDLEGNVCLNILRED
Sbjct: 61  NFEVSIKPDDGYYHNGTFVFTFQVSPVYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRED 120

Query: 121 WKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFF 180
           WKPVLNINT+IYGLFHLFT+PN EDPLNHDAAAVLRDNPKLFE+NVRRAM GGYVGQTFF
Sbjct: 121 WKPVLNINTVIYGLFHLFTEPNSEDPLNHDAAAVLRDNPKLFETNVRRAMTGGYVGQTFF 180

Query: 181 TRCM 184
            RC+
Sbjct: 181 PRCI 184

[Close]

BLAST of MC04g0492 vs. ExPASy Swiss-Prot
Match: Q9ZU75 (Probable NEDD8-conjugating enzyme Ubc12-like OS=Arabidopsis thaliana OX=3702 GN=RCE2 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 8.2e-86
Identity = 153/185 (82.70%), Postives = 165/185 (89.19%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGK--PPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDL 60
           MI LFKVKEKQRE +++ NG+    VKKQSAGELRL KDISELNLPK+C ISFPNGK+DL
Sbjct: 1   MIGLFKVKEKQREESQSNNGRGASTVKKQSAGELRLHKDISELNLPKSCKISFPNGKNDL 60

Query: 61  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 120
           MNFEVTI PDEGYYL G F+FSFQVS +YPHE PKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 61  MNFEVTIKPDEGYYLSGNFVFSFQVSNMYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRE 120

Query: 121 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTF 180
           DWKPVLNINT+IYGLFHLFT+PN+EDPLNH+AAAVLRDNPK FE NVRRAM GG VGQT 
Sbjct: 121 DWKPVLNINTVIYGLFHLFTEPNYEDPLNHEAAAVLRDNPKTFEYNVRRAMMGGQVGQTS 180

Query: 181 FTRCM 184
           F RCM
Sbjct: 181 FPRCM 185

[Close]

BLAST of MC04g0492 vs. ExPASy Swiss-Prot
Match: Q6DCZ9 (NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis OX=8355 GN=ube2m PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-65
Identity = 117/183 (63.93%), Postives = 146/183 (79.78%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLF +K++++E       K   KK SA +LR+QKDI+ELNLPKTC I F +  DDL+N
Sbjct: 1   MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDITELNLPKTCEIEF-SDHDDLLN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           F++ I PDEG+Y GG F+FSF+V   YPH+PPKVKC+T VYHPNIDLEGNVCLNILREDW
Sbjct: 61  FKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVL IN+IIYGL +LF +PN EDPLN +AA VL++N +LFE NV+R+M GGY+G T+F 
Sbjct: 121 KPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFE 180

Query: 181 RCM 184
           RC+
Sbjct: 181 RCL 182

[Close]

BLAST of MC04g0492 vs. ExPASy Swiss-Prot
Match: Q6P8D9 (NEDD8-conjugating enzyme Ubc12 OS=Xenopus tropicalis OX=8364 GN=ube2m PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-65
Identity = 117/183 (63.93%), Postives = 146/183 (79.78%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLF +K++++E       K   KK SA +LR+QKDI+ELNLPKTC I F +  DDL+N
Sbjct: 1   MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCEIEF-SDHDDLLN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           F++ I PDEG+Y GG F+FSF+V   YPH+PPKVKC+T VYHPNIDLEGNVCLNILREDW
Sbjct: 61  FKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVL IN+IIYGL +LF +PN EDPLN +AA VL++N +LFE NV+R+M GGY+G T+F 
Sbjct: 121 KPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFE 180

Query: 181 RCM 184
           RC+
Sbjct: 181 RCL 182

[Close]

BLAST of MC04g0492 vs. ExPASy Swiss-Prot
Match: A3KN22 (NEDD8-conjugating enzyme Ubc12 OS=Bos taurus OX=9913 GN=UBE2M PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.7e-65
Identity = 117/183 (63.93%), Postives = 146/183 (79.78%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLF +K++++E       K   KK SA +LR+QKDI+ELNLPKTC ISF +  DDL+N
Sbjct: 1   MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISF-SDPDDLLN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           F++ I PDEG+Y  G F+FSF+V   YPH+PPKVKC+T VYHPNIDLEGNVCLNILREDW
Sbjct: 61  FKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVL IN+IIYGL +LF +PN EDPLN +AA VL++N +LFE NV+R+M GGY+G T+F 
Sbjct: 121 KPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFE 180

Query: 181 RCM 184
           RC+
Sbjct: 181 RCL 182

[Close]

BLAST of MC04g0492 vs. NCBI nr
Match: XP_022136196.1 (NEDD8-conjugating enzyme Ubc12-like [Momordica charantia])

HSP 1 Score: 382 bits (982), Expect = 3.64e-134
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN
Sbjct: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. NCBI nr
Match: XP_038888942.1 (NEDD8-conjugating enzyme Ubc12-like [Benincasa hispida] >XP_038888944.1 NEDD8-conjugating enzyme Ubc12-like [Benincasa hispida])

HSP 1 Score: 377 bits (968), Expect = 4.97e-132
Identity = 179/183 (97.81%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA NANGKPPVKKQ+AGELRLQKDISELNLPKTCSISFPNGKDDLMN
Sbjct: 1   MIKLFKVKEKQREIAANANGKPPVKKQTAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FESNVRRAMAGGYVGQT+FT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGQTYFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. NCBI nr
Match: XP_023530137.1 (NEDD8-conjugating enzyme Ubc12-like [Cucurbita pepo subsp. pepo] >XP_023530138.1 NEDD8-conjugating enzyme Ubc12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375 bits (962), Expect = 4.09e-131
Identity = 178/183 (97.27%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA+NA+GKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKD+LMN
Sbjct: 1   MIKLFKVKEKQREIAKNADGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDELMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FESNVRRAMAGGYVG TFFT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGHTFFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. NCBI nr
Match: XP_022931623.1 (NEDD8-conjugating enzyme Ubc12-like [Cucurbita moschata] >XP_022931625.1 NEDD8-conjugating enzyme Ubc12-like [Cucurbita moschata] >XP_022989474.1 NEDD8-conjugating enzyme Ubc12-like [Cucurbita maxima] >XP_022989475.1 NEDD8-conjugating enzyme Ubc12-like [Cucurbita maxima])

HSP 1 Score: 374 bits (959), Expect = 1.17e-130
Identity = 177/183 (96.72%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA+NA+GKPPVKKQ+AGELRLQKDISELNLPKTCSISFPNGKD+LMN
Sbjct: 1   MIKLFKVKEKQREIAKNADGKPPVKKQTAGELRLQKDISELNLPKTCSISFPNGKDELMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FESNVRRAMAGGYVG TFFT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGHTFFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. NCBI nr
Match: KAG7022419.1 (NEDD8-conjugating enzyme Ubc12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374 bits (959), Expect = 3.65e-130
Identity = 177/183 (96.72%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA+NA+GKPPVKKQ+AGELRLQKDISELNLPKTCSISFPNGKD+LMN
Sbjct: 33  MIKLFKVKEKQREIAKNADGKPPVKKQTAGELRLQKDISELNLPKTCSISFPNGKDELMN 92

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 93  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 152

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FESNVRRAMAGGYVG TFFT
Sbjct: 153 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGHTFFT 212

Query: 181 RCM 183
           RCM
Sbjct: 213 RCM 215

[Close]

BLAST of MC04g0492 vs. ExPASy TrEMBL
Match: A0A6J1C2V1 (NEDD8-conjugating enzyme Ubc12-like OS=Momordica charantia OX=3673 GN=LOC111007951 PE=3 SV=1)

HSP 1 Score: 382 bits (982), Expect = 1.76e-134
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN
Sbjct: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. ExPASy TrEMBL
Match: A0A6J1JMG9 (NEDD8-conjugating enzyme Ubc12-like OS=Cucurbita maxima OX=3661 GN=LOC111486523 PE=3 SV=1)

HSP 1 Score: 374 bits (959), Expect = 5.67e-131
Identity = 177/183 (96.72%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA+NA+GKPPVKKQ+AGELRLQKDISELNLPKTCSISFPNGKD+LMN
Sbjct: 1   MIKLFKVKEKQREIAKNADGKPPVKKQTAGELRLQKDISELNLPKTCSISFPNGKDELMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FESNVRRAMAGGYVG TFFT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGHTFFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. ExPASy TrEMBL
Match: A0A6J1EZ82 (NEDD8-conjugating enzyme Ubc12-like OS=Cucurbita moschata OX=3662 GN=LOC111437787 PE=3 SV=1)

HSP 1 Score: 374 bits (959), Expect = 5.67e-131
Identity = 177/183 (96.72%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA+NA+GKPPVKKQ+AGELRLQKDISELNLPKTCSISFPNGKD+LMN
Sbjct: 1   MIKLFKVKEKQREIAKNADGKPPVKKQTAGELRLQKDISELNLPKTCSISFPNGKDELMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FESNVRRAMAGGYVG TFFT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGHTFFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. ExPASy TrEMBL
Match: A0A0A0K481 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G253730 PE=3 SV=1)

HSP 1 Score: 372 bits (955), Expect = 2.31e-130
Identity = 177/183 (96.72%), Postives = 180/183 (98.36%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA NANG PPVKKQSAGELRLQKDISELNLPKTC+ISFPNGKDDLMN
Sbjct: 1   MIKLFKVKEKQREIAANANGVPPVKKQSAGELRLQKDISELNLPKTCNISFPNGKDDLMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FE NVRRAMAGGYVGQT+FT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTYFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. ExPASy TrEMBL
Match: A0A1S3BHD5 (NEDD8-conjugating enzyme Ubc12-like OS=Cucumis melo OX=3656 GN=LOC103489610 PE=3 SV=1)

HSP 1 Score: 372 bits (955), Expect = 2.31e-130
Identity = 177/183 (96.72%), Postives = 180/183 (98.36%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60
           MIKLFKVKEKQREIA NANG PPVKKQ+AGELRLQKDISELNLPKTCSISFPNGKDDLMN
Sbjct: 1   MIKLFKVKEKQREIAANANGVPPVKKQTAGELRLQKDISELNLPKTCSISFPNGKDDLMN 60

Query: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120
           FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW
Sbjct: 61  FEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDW 120

Query: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFFT 180
           KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FE NVRRAMAGGYVGQT+FT
Sbjct: 121 KPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTYFT 180

Query: 181 RCM 183
           RCM
Sbjct: 181 RCM 183

[Close]

BLAST of MC04g0492 vs. TAIR 10
Match: AT4G36800.1 (RUB1 conjugating enzyme 1 )

HSP 1 Score: 329.7 bits (844), Expect = 1.5e-90
Identity = 158/184 (85.87%), Postives = 168/184 (91.30%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENA-NGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLM 60
           MI LFKVKEKQRE A+NA  G   VKKQSAGELRL KDISELNLP +CSISFPNGKDDLM
Sbjct: 1   MIGLFKVKEKQREQAQNATRGGASVKKQSAGELRLHKDISELNLPSSCSISFPNGKDDLM 60

Query: 61  NFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRED 120
           NFEV+I PD+GYY  GTF+F+FQVSPVYPHE PKVKCKTKVYHPNIDLEGNVCLNILRED
Sbjct: 61  NFEVSIKPDDGYYHNGTFVFTFQVSPVYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRED 120

Query: 121 WKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFF 180
           WKPVLNINT+IYGLFHLFT+PN EDPLNHDAAAVLRDNPKLFE+NVRRAM GGYVGQTFF
Sbjct: 121 WKPVLNINTVIYGLFHLFTEPNSEDPLNHDAAAVLRDNPKLFETNVRRAMTGGYVGQTFF 180

Query: 181 TRCM 184
            RC+
Sbjct: 181 PRCI 184

[Close]

BLAST of MC04g0492 vs. TAIR 10
Match: AT4G36800.2 (RUB1 conjugating enzyme 1 )

HSP 1 Score: 329.7 bits (844), Expect = 1.5e-90
Identity = 158/184 (85.87%), Postives = 168/184 (91.30%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENA-NGKPPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDLM 60
           MI LFKVKEKQRE A+NA  G   VKKQSAGELRL KDISELNLP +CSISFPNGKDDLM
Sbjct: 1   MIGLFKVKEKQREQAQNATRGGASVKKQSAGELRLHKDISELNLPSSCSISFPNGKDDLM 60

Query: 61  NFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRED 120
           NFEV+I PD+GYY  GTF+F+FQVSPVYPHE PKVKCKTKVYHPNIDLEGNVCLNILRED
Sbjct: 61  NFEVSIKPDDGYYHNGTFVFTFQVSPVYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRED 120

Query: 121 WKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTFF 180
           WKPVLNINT+IYGLFHLFT+PN EDPLNHDAAAVLRDNPKLFE+NVRRAM GGYVGQTFF
Sbjct: 121 WKPVLNINTVIYGLFHLFTEPNSEDPLNHDAAAVLRDNPKLFETNVRRAMTGGYVGQTFF 180

Query: 181 TRCM 184
            RC+
Sbjct: 181 PRCI 184

[Close]

BLAST of MC04g0492 vs. TAIR 10
Match: AT2G18600.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 317.8 bits (813), Expect = 5.8e-87
Identity = 153/185 (82.70%), Postives = 165/185 (89.19%), Query Frame = 0


Query: 1   MIKLFKVKEKQREIAENANGK--PPVKKQSAGELRLQKDISELNLPKTCSISFPNGKDDL 60
           MI LFKVKEKQRE +++ NG+    VKKQSAGELRL KDISELNLPK+C ISFPNGK+DL
Sbjct: 1   MIGLFKVKEKQREESQSNNGRGASTVKKQSAGELRLHKDISELNLPKSCKISFPNGKNDL 60

Query: 61  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 120
           MNFEVTI PDEGYYL G F+FSFQVS +YPHE PKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 61  MNFEVTIKPDEGYYLSGNFVFSFQVSNMYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRE 120

Query: 121 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTF 180
           DWKPVLNINT+IYGLFHLFT+PN+EDPLNH+AAAVLRDNPK FE NVRRAM GG VGQT 
Sbjct: 121 DWKPVLNINTVIYGLFHLFTEPNYEDPLNHEAAAVLRDNPKTFEYNVRRAMMGGQVGQTS 180

Query: 181 FTRCM 184
           F RCM
Sbjct: 181 FPRCM 185

[Close]

BLAST of MC04g0492 vs. TAIR 10
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 109.8 bits (273), Expect = 2.4e-24
Identity = 52/135 (38.52%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 33  RLQKDISELNLPKTCSISFPNGKDDLMNFEVTI-HPDEGYYLGGTFLFSFQVSPVYPHEP 92
           R+ K++ +L      + S     +D+ +++ TI  P E  Y GG FL S    P YP +P
Sbjct: 5   RILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKP 64

Query: 93  PKVKCKTKVYHPNIDLEGNVCLNILREDWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAA 152
           PKV  KTKVYHPNI+  G++CL+IL+E W P L I+ ++  +  L T PN +DPL  + A
Sbjct: 65  PKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 124

Query: 153 AVLRDNPKLFESNVR 167
            + + +   +ES  R
Sbjct: 125 HMYKTDRSKYESTAR 139

[Close]

BLAST of MC04g0492 vs. TAIR 10
Match: AT3G08690.2 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 109.8 bits (273), Expect = 2.4e-24
Identity = 52/135 (38.52%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 33  RLQKDISELNLPKTCSISFPNGKDDLMNFEVTI-HPDEGYYLGGTFLFSFQVSPVYPHEP 92
           R+ K++ +L      + S     +D+ +++ TI  P E  Y GG FL S    P YP +P
Sbjct: 5   RILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKP 64

Query: 93  PKVKCKTKVYHPNIDLEGNVCLNILREDWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAA 152
           PKV  KTKVYHPNI+  G++CL+IL+E W P L I+ ++  +  L T PN +DPL  + A
Sbjct: 65  PKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 124

Query: 153 AVLRDNPKLFESNVR 167
            + + +   +ES  R
Sbjct: 125 HMYKTDRSKYESTAR 139

The following BLAST results are available for this feature:

BLAST of MC04g0492 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDY5	2.1e-89	85.87	NEDD8-conjugating enzyme Ubc12 OS=Arabidopsis thaliana OX=3702 GN=RCE1 PE=1 SV=1	[more]
Q9ZU75	8.2e-86	82.70	Probable NEDD8-conjugating enzyme Ubc12-like OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q6DCZ9	1.6e-65	63.93	NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis OX=8355 GN=ube2m PE=2 SV=1	[more]
Q6P8D9	1.6e-65	63.93	NEDD8-conjugating enzyme Ubc12 OS=Xenopus tropicalis OX=8364 GN=ube2m PE=2 SV=1	[more]
A3KN22	2.7e-65	63.93	NEDD8-conjugating enzyme Ubc12 OS=Bos taurus OX=9913 GN=UBE2M PE=2 SV=1	[more]

BLAST of MC04g0492 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136196.1	3.64e-134	100.00	NEDD8-conjugating enzyme Ubc12-like [Momordica charantia]	[more]
XP_038888942.1	4.97e-132	97.81	NEDD8-conjugating enzyme Ubc12-like [Benincasa hispida] >XP_038888944.1 NEDD8-co...	[more]
XP_023530137.1	4.09e-131	97.27	NEDD8-conjugating enzyme Ubc12-like [Cucurbita pepo subsp. pepo] >XP_023530138.1...	[more]
XP_022931623.1	1.17e-130	96.72	NEDD8-conjugating enzyme Ubc12-like [Cucurbita moschata] >XP_022931625.1 NEDD8-c...	[more]
KAG7022419.1	3.65e-130	96.72	NEDD8-conjugating enzyme Ubc12 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC04g0492 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2V1	1.76e-134	100.00	NEDD8-conjugating enzyme Ubc12-like OS=Momordica charantia OX=3673 GN=LOC1110079...	[more]
A0A6J1JMG9	5.67e-131	96.72	NEDD8-conjugating enzyme Ubc12-like OS=Cucurbita maxima OX=3661 GN=LOC111486523 ...	[more]
A0A6J1EZ82	5.67e-131	96.72	NEDD8-conjugating enzyme Ubc12-like OS=Cucurbita moschata OX=3662 GN=LOC11143778...	[more]
A0A0A0K481	2.31e-130	96.72	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G253730 PE...	[more]
A0A1S3BHD5	2.31e-130	96.72	NEDD8-conjugating enzyme Ubc12-like OS=Cucumis melo OX=3656 GN=LOC103489610 PE=3...	[more]

BLAST of MC04g0492 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36800.1	1.5e-90	85.87	RUB1 conjugating enzyme 1	[more]
AT4G36800.2	1.5e-90	85.87	RUB1 conjugating enzyme 1	[more]
AT2G18600.1	5.8e-87	82.70	Ubiquitin-conjugating enzyme family protein	[more]
AT3G08690.1	2.4e-24	38.52	ubiquitin-conjugating enzyme 11	[more]
AT3G08690.2	2.4e-24	38.52	ubiquitin-conjugating enzyme 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 32..174 e-value: 7.7E-38 score: 141.7
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 10..173
None	No IPR available	PANTHER	PTHR24068:SF379	SUBFAMILY NOT NAMED	coord: 10..173
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 25..183 e-value: 3.8E-48 score: 165.2
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 21..179
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 33..168 e-value: 4.6E-39 score: 133.3
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 29..174 score: 38.123051
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 33..167 e-value: 7.84067E-41 score: 133.096
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 101..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0492.1	MC04g0492.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0019788	NEDD8 transferase activity