MC04g0404 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0404 vs. ExPASy Swiss-Prot
Match: Q554C9 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0275203 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 9.2e-60
Identity = 142/354 (40.11%), Postives = 199/354 (56.21%), Query Frame = 0

Query: 2   TRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRP--RIEWTDFNATRE 61
           T+ KRK R   ++H  N Y  NPP+F LLAS YP+F  ++         I+W D NAT+E
Sbjct: 31  TKKKRK-RSNDSMHINNFYRYNPPNFKLLASKYPTFDKYIINKTEKIYNIDWKDSNATKE 90

Query: 62  LTRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSS-DIIANTN-SDRGCVRGFD 121
           LTRVLL HD GL   +PD  LCPT+  R NY++W+ D L +  II N N +D   ++G D
Sbjct: 91  LTRVLLDHDFGLRIELPDNYLCPTLTLRINYLYWISDQLKNLKIILNDNDNDNKIIKGID 150

Query: 122 IGTGANCIYPLLGASLL-GWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNT 181
           IGTG +CI+PLLGA L   WSF+G D+ D  LE+A+ N+  N  ++  + + K +  S+ 
Sbjct: 151 IGTGTSCIFPLLGAKLFNNWSFIGIDIDDKVLEYAQNNITIN-SLNSKITLFKNEKNSDI 210

Query: 182 LSTELHDGVAVNS-ESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGE 241
           L   L+     N+  S N D  D                              D   D E
Sbjct: 211 LLKLLNYKEGSNTFNSSNDDHQDNH----------------------------DDDDDDE 270

Query: 242 KF--DFCMCNPPFFASMDE----AGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLK 301
           ++  DFC+CNPPFF  ++E       NPK++C G++ EMV  GGE  F+ R+I++S  LK
Sbjct: 271 EYFADFCLCNPPFFKDLNENNNNKNNNPKSNCTGSVNEMVTDGGEFEFVKRIIKESFQLK 330

Query: 302 QTFRWYTSMIGKKSNLSFLIS---KLWKVGVTVVKTTEFVQGQTSRWGLAWSFM 341
              R+YT+MIG+K NL+ LI+   K + +    ++TTE VQG TSRW L+W F+
Sbjct: 331 CKIRFYTTMIGRKVNLNPLINILIKQYYLPKNQIQTTELVQGNTSRWVLSWYFL 354

BLAST of MC04g0404 vs. ExPASy Swiss-Prot
Match: Q6DC64 (RNA N6-adenosine-methyltransferase mettl16 OS=Danio rerio OX=7955 GN=mettl16 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 5.6e-57
Identity = 135/339 (39.82%), Postives = 179/339 (52.80%), Query Frame = 0

Query: 13  TIHPRNKYAENPPDFALLASLYPSFQPFV--FLHPRPRIEWTDFNATRELTRVLLLHDHG 72
           ++HPRN+Y + PPDF  LAS YP FQ  V   L  R  + + D  A R LT  LL  D G
Sbjct: 6   SMHPRNRYKDKPPDFVYLASKYPEFQKHVQTTLTGRVTLNFKDPEAVRALTCTLLKEDFG 65

Query: 73  LNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGTGANCIYPLLG 132
           L   IP  +L PTVP R NYIHW+EDL+      N        RG DIGTGA+CIYPLLG
Sbjct: 66  LTIEIPLERLIPTVPLRLNYIHWVEDLIGGQ--GNPQ------RGIDIGTGASCIYPLLG 125

Query: 133 ASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTELHDGVAVNSE 192
           A++ GW F+ T+V D+   +A+ NV+ N H++EL+++ KV   +                
Sbjct: 126 ATMNGWFFLATEVDDICFNYAKKNVEQN-HLAELIKVVKVPQKT---------------- 185

Query: 193 SKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGE-KFDFCMCNPPFFAS 252
                                              +L+D +K+    +DFCMCNPPFFA+
Sbjct: 186 -----------------------------------LLMDALKEESIVYDFCMCNPPFFAN 245

Query: 253 MDEA-GLN--------PKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 312
             EA G+N        P +   G + E++  GGE  F+ R+I DS  LK+  RWY+ M+G
Sbjct: 246 QLEAKGVNSRNSRRPPPSSINTGGVTEIMAEGGELEFVKRVIHDSLQLKKRLRWYSCMLG 284

Query: 313 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSF 340
           KK +L+ L  +L K GV  V  TEF QG+T RW LAWSF
Sbjct: 306 KKCSLAPLKDELRKQGVAKVTHTEFCQGRTMRWALAWSF 284

BLAST of MC04g0404 vs. ExPASy Swiss-Prot
Match: Q86W50 (RNA N6-adenosine-methyltransferase METTL16 OS=Homo sapiens OX=9606 GN=METTL16 PE=1 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 2.8e-56
Identity = 135/341 (39.59%), Postives = 186/341 (54.55%), Query Frame = 0

Query: 13  TIHPRNKYAENPPDFALLASLYPSFQPFV--FLHPRPRIEWTDFNATRELTRVLLLHDHG 72
           ++H RN+Y + PPDFA LAS YP F+  V   L+ R  + + D  A R LT  LL  D G
Sbjct: 6   SMHARNRYKDKPPDFAYLASKYPDFKQHVQINLNGRVSLNFKDPEAVRALTCTLLREDFG 65

Query: 73  LNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCV-RGFDIGTGANCIYPLL 132
           L+  IP  +L PTVP R NYIHW+EDL     I + +SD+  + RG DIGTGA+CIYPLL
Sbjct: 66  LSIDIPLERLIPTVPLRLNYIHWVEDL-----IGHQDSDKSTLRRGIDIGTGASCIYPLL 125

Query: 133 GASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTELHDGVAVNS 192
           GA+L GW F+ T+V D+   +A+ NV+ N ++S+L+++ KV   +               
Sbjct: 126 GATLNGWYFLATEVDDMCFNYAKKNVEQN-NLSDLIKVVKVPQKT--------------- 185

Query: 193 ESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEK--FDFCMCNPPFF 252
                                               +L+D +K+  +  +DFCMCNPPFF
Sbjct: 186 ------------------------------------LLMDALKEESEIIYDFCMCNPPFF 245

Query: 253 ASMDEA-GLN--------PKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSM 312
           A+  EA G+N        P +   G + E++  GGE  F+ R+I DS  LK+  RWY+ M
Sbjct: 246 ANQLEAKGVNSRNPRRPPPSSVNTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSCM 289

Query: 313 IGKKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSF 340
           +GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF
Sbjct: 306 LGKKCSLAPLKEELRIQGVPKVTYTEFCQGRTMRWALAWSF 289

BLAST of MC04g0404 vs. ExPASy Swiss-Prot
Match: Q6GR37 (RNA N6-adenosine-methyltransferase mettl16 OS=Xenopus laevis OX=8355 GN=mettl16 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 4.7e-56
Identity = 135/341 (39.59%), Postives = 185/341 (54.25%), Query Frame = 0

Query: 13  TIHPRNKYAENPPDFALLASLYPSFQPF--VFLHPRPRIEWTDFNATRELTRVLLLHDHG 72
           ++HPRN+Y + PPDFA LAS YP F+    V L  R  + + D  A R LT  LL  D G
Sbjct: 6   SMHPRNRYKDKPPDFAYLASKYPEFKQHVNVNLAGRVSLNFKDPCAVRALTCTLLKEDFG 65

Query: 73  LNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCV-RGFDIGTGANCIYPLL 132
           L   IP  +L PTVP R NYIHW+EDL++       +SD+  + RG DIGTGA+CIYPLL
Sbjct: 66  LTIDIPLERLIPTVPLRLNYIHWVEDLINYH-----DSDKTALRRGIDIGTGASCIYPLL 125

Query: 133 GASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTELHDGVAVNS 192
           GA+L GW F+ T+V D+   +A+ NV+ N ++S+L+++ KV   +               
Sbjct: 126 GATLNGWYFLATEVDDICYNYAKKNVEQN-NLSDLIKVVKVPQKT--------------- 185

Query: 193 ESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEK--FDFCMCNPPFF 252
                                               +L+D +K+  +  +DFCMCNPPFF
Sbjct: 186 ------------------------------------LLMDALKEESEIIYDFCMCNPPFF 245

Query: 253 ASMDEA-GLN--------PKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSM 312
           A+  EA G+N        P +   G + E++  GGE  F+ R+I DS  LK+  RWY+ M
Sbjct: 246 ANQLEAQGVNSRNPHRSPPSSVNTGGITEIMAEGGELEFVKRIIHDSLKLKKRLRWYSCM 289

Query: 313 IGKKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSF 340
           +GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF
Sbjct: 306 LGKKCSLAPLKEELRLQGVPKVAHTEFYQGRTMRWALAWSF 289

BLAST of MC04g0404 vs. ExPASy Swiss-Prot
Match: Q9CQG2 (RNA N6-adenosine-methyltransferase METTL16 OS=Mus musculus OX=10090 GN=Mettl16 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 6.2e-56
Identity = 134/341 (39.30%), Postives = 186/341 (54.55%), Query Frame = 0

Query: 13  TIHPRNKYAENPPDFALLASLYPSFQPF--VFLHPRPRIEWTDFNATRELTRVLLLHDHG 72
           ++H RN+Y + PPDFA LAS YP F+    + L+ R  + + D  A R LT  LL  D G
Sbjct: 6   SMHARNRYKDKPPDFAYLASKYPDFKQHIQINLNGRVSLNFKDPEAVRALTCTLLREDFG 65

Query: 73  LNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCV-RGFDIGTGANCIYPLL 132
           L+  IP  +L PTVP R NYIHW+EDL     I + +SD+  + RG DIGTGA+CIYPLL
Sbjct: 66  LSIDIPLERLIPTVPLRLNYIHWVEDL-----IGHQDSDKTTLRRGIDIGTGASCIYPLL 125

Query: 133 GASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTELHDGVAVNS 192
           GA+L GW F+ T+V D+   +A+ NV+ N ++S+L+++ KV   +               
Sbjct: 126 GATLNGWYFLATEVDDMCFNYAKKNVEQN-NLSDLIKVVKVPQKT--------------- 185

Query: 193 ESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEK--FDFCMCNPPFF 252
                                               +L+D +K+  +  +DFCMCNPPFF
Sbjct: 186 ------------------------------------LLMDALKEESEIVYDFCMCNPPFF 245

Query: 253 ASMDEA-GLN--------PKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSM 312
           A+  EA G+N        P +   G + E++  GGE  F+ R+I DS  LK+  RWY+ M
Sbjct: 246 ANQLEAKGVNSRNSRRPPPSSVNTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSCM 289

Query: 313 IGKKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSF 340
           +GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF
Sbjct: 306 LGKKCSLAPLKEELRIQGVPKVTFTEFCQGRTMRWALAWSF 289

BLAST of MC04g0404 vs. NCBI nr
Match: XP_022135953.1 (methyltransferase-like protein 16 homolog isoform X1 [Momordica charantia] >XP_022135955.1 methyltransferase-like protein 16 homolog isoform X1 [Momordica charantia])

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL
Sbjct: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120
           TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT
Sbjct: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180

Query: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240
           LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC
Sbjct: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240

Query: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360

Query: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420
           LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG
Sbjct: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420

Query: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480
           AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK
Sbjct: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480

Query: 481 AESCREKVS 489
           AESCREKVS
Sbjct: 481 AESCREKVS 489

BLAST of MC04g0404 vs. NCBI nr
Match: XP_038886852.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 845 bits (2183), Expect = 6.18e-307
Identity = 418/488 (85.66%), Postives = 442/488 (90.57%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLLSSDI+A  NSD G VRG DIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIVAKKNSDSGPVRGLDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVD  S+ LSTE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDISDDLSTE 180

Query: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240
           LH+ VAV+SE K  ++ DG  V PP PSS   ETV+LEKRYHGPPILL VVKDGE+FDFC
Sbjct: 181 LHNSVAVDSEFKILNEKDGMEVAPPLPSSLSKETVHLEKRYHGPPILLGVVKDGEEFDFC 240

Query: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFF SMDEAGLNPKTSCGGT QEMVCPGGERAFISRMIEDS VL+QTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSIVLRQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQ  RWGLAWSFMP ARKIIS HVTNKSV+SFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQICRWGLAWSFMPTARKIISPHVTNKSVMSFM 360

Query: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420
           LEGLLP+V+A SILQS+E FLS  GASCKLNTSSFTVDITASEDHC+AILKN A +VDKG
Sbjct: 361 LEGLLPQVSATSILQSVERFLSASGASCKLNTSSFTVDITASEDHCRAILKNRACEVDKG 420

Query: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480
           A   TV++SE QPS++LCFRVSVFQQIPG+LLVKGSLHH SS SAG FSLIFH LEEFLK
Sbjct: 421 ASSATVEKSEPQPSSDLCFRVSVFQQIPGSLLVKGSLHHKSSPSAGSFSLIFHGLEEFLK 480

Query: 481 AESCREKV 488
           AESC+EKV
Sbjct: 481 AESCKEKV 488

BLAST of MC04g0404 vs. NCBI nr
Match: XP_022969493.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita maxima])

HSP 1 Score: 845 bits (2183), Expect = 6.42e-307
Identity = 417/489 (85.28%), Postives = 443/489 (90.59%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGC-VRGFDIG 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLLSSD++A  N DRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANRDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLST 180
           TGANCIYPLLGASLLGWSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVD T + L+T
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 181 ELHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDF 240
           EL   VAV++E K  + MDGR VGP  PSSS  ET++ EKRYHGPPILL VVKDGE+FDF
Sbjct: 181 ELRSNVAVDNERKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFF SMDEAGLNPKT+CGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFMPA RKIIS HVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDK 420
           MLEGLLP+V+AMSILQS+E FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGAY+VDK
Sbjct: 361 MLEGLLPQVSAMSILQSVERFLSAGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFL 480
           GA  V V++SE QPS +LCFRVSVFQQIPGTLLVKGSLHH SS S+G FS IFHRLEEFL
Sbjct: 421 GASGVPVEKSEPQPSTDLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCREKV 488
           KAESC+EKV
Sbjct: 481 KAESCKEKV 489

BLAST of MC04g0404 vs. NCBI nr
Match: XP_008447969.1 (PREDICTED: methyltransferase-like protein 16 homolog isoform X2 [Cucumis melo])

HSP 1 Score: 845 bits (2182), Expect = 7.55e-307
Identity = 416/485 (85.77%), Postives = 443/485 (91.34%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLL+SDI+A  NSD G VRGFDIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLASDIVAKKNSDCGRVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVD TS+ LSTE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAEKNVKSNPHVSELIEIRKVDDTSDNLSTE 180

Query: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240
           LHD VAV+SE K  ++M GR VGPP PS  L E V+LEK+YHGPPILL VVKDGE+FDFC
Sbjct: 181 LHDSVAVDSECKIFNEMGGREVGPPLPSFLLKEAVHLEKQYHGPPILLGVVKDGEEFDFC 240

Query: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFF SMDEAGLNPKTSCGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTSQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFM  ARKIIS HVTNKS+LSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMSTARKIISPHVTNKSILSFM 360

Query: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420
           LEGLLP+V+AMSILQS+E FLSTGGASCKLNTSSFTVDITAS DHC+AI++NGA Q DK 
Sbjct: 361 LEGLLPQVSAMSILQSVERFLSTGGASCKLNTSSFTVDITASVDHCRAIIENGACQEDKV 420

Query: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480
           A CV +++SE+Q S +LCFRVSVFQQIPGTLLVKGSLH  SS SAG+FSLIFHRLEEFLK
Sbjct: 421 ASCVALEKSETQLSTDLCFRVSVFQQIPGTLLVKGSLHQKSSPSAGLFSLIFHRLEEFLK 480

Query: 481 AESCR 485
           A+ C+
Sbjct: 481 ADFCK 485

BLAST of MC04g0404 vs. NCBI nr
Match: XP_022952458.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita moschata])

HSP 1 Score: 840 bits (2170), Expect = 6.13e-305
Identity = 415/489 (84.87%), Postives = 442/489 (90.39%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGC-VRGFDIG 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLLSSD++A  NSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLST 180
           TGANCIYPLLGASLLGWSFVGTDVT+VALEWAE NVKSNPHVSEL+EIRKVD T + L+ 
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTNVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTI 180

Query: 181 ELHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDF 240
           EL   VAV++E K  + MDGR VGP  PSSS  ET++ EKRYHGPPILL VVKDGE+FDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFF SMDEAGLNPKT+CGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFMPA RKIIS HVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDK 420
           MLEGLLP+V+A SILQS+E FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGAY+VDK
Sbjct: 361 MLEGLLPQVSATSILQSVERFLSVGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFL 480
           GA  V V++SE QPS +LCFRVSVFQQIPGTLLVKGSLHH SS S+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEKSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCREKV 488
           KAESC+EKV
Sbjct: 481 KAESCKEKV 489

BLAST of MC04g0404 vs. ExPASy TrEMBL
Match: A0A6J1C2I0 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111007785 PE=3 SV=1)

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL
Sbjct: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120
           TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT
Sbjct: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180

Query: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240
           LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC
Sbjct: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240

Query: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360

Query: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420
           LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG
Sbjct: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420

Query: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480
           AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK
Sbjct: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480

Query: 481 AESCREKVS 489
           AESCREKVS
Sbjct: 481 AESCREKVS 489

BLAST of MC04g0404 vs. ExPASy TrEMBL
Match: A0A6J1I146 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468473 PE=3 SV=1)

HSP 1 Score: 845 bits (2183), Expect = 3.11e-307
Identity = 417/489 (85.28%), Postives = 443/489 (90.59%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGC-VRGFDIG 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLLSSD++A  N DRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANRDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLST 180
           TGANCIYPLLGASLLGWSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVD T + L+T
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 181 ELHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDF 240
           EL   VAV++E K  + MDGR VGP  PSSS  ET++ EKRYHGPPILL VVKDGE+FDF
Sbjct: 181 ELRSNVAVDNERKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFF SMDEAGLNPKT+CGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFMPA RKIIS HVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDK 420
           MLEGLLP+V+AMSILQS+E FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGAY+VDK
Sbjct: 361 MLEGLLPQVSAMSILQSVERFLSAGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFL 480
           GA  V V++SE QPS +LCFRVSVFQQIPGTLLVKGSLHH SS S+G FS IFHRLEEFL
Sbjct: 421 GASGVPVEKSEPQPSTDLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCREKV 488
           KAESC+EKV
Sbjct: 481 KAESCKEKV 489

BLAST of MC04g0404 vs. ExPASy TrEMBL
Match: A0A1S3BIM5 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103490299 PE=3 SV=1)

HSP 1 Score: 845 bits (2182), Expect = 3.66e-307
Identity = 416/485 (85.77%), Postives = 443/485 (91.34%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLL+SDI+A  NSD G VRGFDIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLASDIVAKKNSDCGRVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVD TS+ LSTE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAEKNVKSNPHVSELIEIRKVDDTSDNLSTE 180

Query: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240
           LHD VAV+SE K  ++M GR VGPP PS  L E V+LEK+YHGPPILL VVKDGE+FDFC
Sbjct: 181 LHDSVAVDSECKIFNEMGGREVGPPLPSFLLKEAVHLEKQYHGPPILLGVVKDGEEFDFC 240

Query: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFF SMDEAGLNPKTSCGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTSQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFM  ARKIIS HVTNKS+LSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMSTARKIISPHVTNKSILSFM 360

Query: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420
           LEGLLP+V+AMSILQS+E FLSTGGASCKLNTSSFTVDITAS DHC+AI++NGA Q DK 
Sbjct: 361 LEGLLPQVSAMSILQSVERFLSTGGASCKLNTSSFTVDITASVDHCRAIIENGACQEDKV 420

Query: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480
           A CV +++SE+Q S +LCFRVSVFQQIPGTLLVKGSLH  SS SAG+FSLIFHRLEEFLK
Sbjct: 421 ASCVALEKSETQLSTDLCFRVSVFQQIPGTLLVKGSLHQKSSPSAGLFSLIFHRLEEFLK 480

Query: 481 AESCR 485
           A+ C+
Sbjct: 481 ADFCK 485

BLAST of MC04g0404 vs. ExPASy TrEMBL
Match: A0A6J1GKM3 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455141 PE=3 SV=1)

HSP 1 Score: 840 bits (2170), Expect = 2.97e-305
Identity = 415/489 (84.87%), Postives = 442/489 (90.39%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFVFL PRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGC-VRGFDIG 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLLSSD++A  NSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLST 180
           TGANCIYPLLGASLLGWSFVGTDVT+VALEWAE NVKSNPHVSEL+EIRKVD T + L+ 
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTNVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTI 180

Query: 181 ELHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDF 240
           EL   VAV++E K  + MDGR VGP  PSSS  ET++ EKRYHGPPILL VVKDGE+FDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFF SMDEAGLNPKT+CGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFMPA RKIIS HVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDK 420
           MLEGLLP+V+A SILQS+E FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGAY+VDK
Sbjct: 361 MLEGLLPQVSATSILQSVERFLSVGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFL 480
           GA  V V++SE QPS +LCFRVSVFQQIPGTLLVKGSLHH SS S+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEKSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCREKV 488
           KAESC+EKV
Sbjct: 481 KAESCKEKV 489

BLAST of MC04g0404 vs. ExPASy TrEMBL
Match: A0A0A0K5X1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G009800 PE=3 SV=1)

HSP 1 Score: 833 bits (2151), Expect = 1.93e-302
Identity = 409/485 (84.33%), Postives = 438/485 (90.31%), Query Frame = 0

Query: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60
           MTR KRKTREA TIHPRNKYAENPPDFALLASLYPSFQPFV LHPRPRI+WTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVSLHPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLLSSD++A  NSD G VRGFDIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKKNSDCGRVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAE NV SNPH+SEL+EIRKVD TS+ LSTE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVTSNPHISELIEIRKVDDTSDDLSTE 180

Query: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240
           LHD VAV+SE K  ++MDGR +GPP PS    E ++LE +YHGPPILL VVKDGE+FDFC
Sbjct: 181 LHDSVAVDSECKIFNEMDGREIGPPLPSFLSKEAIHLETQYHGPPILLGVVKDGERFDFC 240

Query: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFF SMDEAGLNPKTSCGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFM  ARKIIS HVTNKS+LSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMSTARKIISPHVTNKSILSFM 360

Query: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420
           LEGLL +V+AMSILQS+E FLSTGGASCKLN SSFTVDITASEDHCQAIL+NGA +  K 
Sbjct: 361 LEGLLSQVSAMSILQSVERFLSTGGASCKLNASSFTVDITASEDHCQAILENGACEEVKD 420

Query: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480
           A CV +++SE+Q S +LCFRVSVFQQIPGTLLVKGSLH  S+ SAG+FSLIFHRLEEFLK
Sbjct: 421 ASCVALEKSETQLSTDLCFRVSVFQQIPGTLLVKGSLHQKSNPSAGLFSLIFHRLEEFLK 480

Query: 481 AESCR 485
           AE C+
Sbjct: 481 AEFCK 485

BLAST of MC04g0404 vs. TAIR 10
Match: AT2G21070.1 (methyltransferases )

HSP 1 Score: 573.9 bits (1478), Expect = 1.2e-163
Identity = 292/489 (59.71%), Postives = 356/489 (72.80%), Query Frame = 0

Query: 3   RNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLH-PRPRIEWTDFNATRELT 62
           + + ++    T  PR+KY++NPPDFA LASLYPSF+PFVF    R RI+WTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 63  RVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGTG 122
           RVLLLHDHG+NWWIPDGQLCPTVPNRSNYIHW+ DLLSS II +   D   V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 123 ANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTEL 182
           ANCIYPLLGASL GWSFVG+D T VALEWAE NV+SNPH S+L+EIR  DS      + +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIR--DSKVPPQCSSV 184

Query: 183 HDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFCM 242
            +    N+E + +   +         S+++    +  K +  P +LL VVK+ E FDFCM
Sbjct: 185 PE--VENTEREKTIQEEAEI------SATVKSDYHDNKSFIEPAVLLGVVKENETFDFCM 244

Query: 243 CNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGK 302
            NPPFF + +EAGLNPKTSCGGT +EMVC GGE+AF+SR+I+DS VL+Q FRWYTSM+GK
Sbjct: 245 SNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGK 304

Query: 303 KSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFML 362
           K+NL  LISKLW+VGVT+VKTTEFVQGQTSRWGLAWSFMP ARKII+  V  KSVLSFML
Sbjct: 305 KANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFML 364

Query: 363 EGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKGA 422
           E +  + +A+ +LQS+E F  + GASCKLN+S+F+VDI AS D C  I KN    VD   
Sbjct: 365 ECIKRQYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKNDIADVDSVR 424

Query: 423 GCVTVKE-----SESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLE 482
                K+     S   P  NL FR+ VFQQ+PGTLL+KGSL    S  +G+FS +F  LE
Sbjct: 425 SHGYEKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSRVFGSLE 483

Query: 483 EFLKAESCR 486
           E LK++ CR
Sbjct: 485 ESLKSKFCR 483

BLAST of MC04g0404 vs. TAIR 10
Match: AT2G21070.3 (methyltransferases )

HSP 1 Score: 573.9 bits (1478), Expect = 1.2e-163
Identity = 292/489 (59.71%), Postives = 356/489 (72.80%), Query Frame = 0

Query: 3   RNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLH-PRPRIEWTDFNATRELT 62
           + + ++    T  PR+KY++NPPDFA LASLYPSF+PFVF    R RI+WTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 63  RVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGTG 122
           RVLLLHDHG+NWWIPDGQLCPTVPNRSNYIHW+ DLLSS II +   D   V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 123 ANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTEL 182
           ANCIYPLLGASL GWSFVG+D T VALEWAE NV+SNPH S+L+EIR  DS      + +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIR--DSKVPPQCSSV 184

Query: 183 HDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFCM 242
            +    N+E + +   +         S+++    +  K +  P +LL VVK+ E FDFCM
Sbjct: 185 PE--VENTEREKTIQEEAEI------SATVKSDYHDNKSFIEPAVLLGVVKENETFDFCM 244

Query: 243 CNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGK 302
            NPPFF + +EAGLNPKTSCGGT +EMVC GGE+AF+SR+I+DS VL+Q FRWYTSM+GK
Sbjct: 245 SNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGK 304

Query: 303 KSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFML 362
           K+NL  LISKLW+VGVT+VKTTEFVQGQTSRWGLAWSFMP ARKII+  V  KSVLSFML
Sbjct: 305 KANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFML 364

Query: 363 EGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKGA 422
           E +  + +A+ +LQS+E F  + GASCKLN+S+F+VDI AS D C  I KN    VD   
Sbjct: 365 ECIKRQYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKNDIADVDSVR 424

Query: 423 GCVTVKE-----SESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLE 482
                K+     S   P  NL FR+ VFQQ+PGTLL+KGSL    S  +G+FS +F  LE
Sbjct: 425 SHGYEKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSRVFGSLE 483

Query: 483 EFLKAESCR 486
           E LK++ CR
Sbjct: 485 ESLKSKFCR 483

BLAST of MC04g0404 vs. TAIR 10
Match: AT2G21070.2 (methyltransferases )

HSP 1 Score: 484.6 bits (1246), Expect = 9.6e-137
Identity = 239/382 (62.57%), Postives = 291/382 (76.18%), Query Frame = 0

Query: 3   RNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLH-PRPRIEWTDFNATRELT 62
           + + ++    T  PR+KY++NPPDFA LASLYPSF+PFVF    R RI+WTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 63  RVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGTG 122
           RVLLLHDHG+NWWIPDGQLCPTVPNRSNYIHW+ DLLSS II +   D   V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 123 ANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTEL 182
           ANCIYPLLGASL GWSFVG+D T VALEWAE NV+SNPH S+L+EIR  DS      + +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIR--DSKVPPQCSSV 184

Query: 183 HDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFCM 242
            +    N+E + +   +         S+++    +  K +  P +LL VVK+ E FDFCM
Sbjct: 185 PE--VENTEREKTIQEEAEI------SATVKSDYHDNKSFIEPAVLLGVVKENETFDFCM 244

Query: 243 CNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGK 302
            NPPFF + +EAGLNPKTSCGGT +EMVC GGE+AF+SR+I+DS VL+Q FRWYTSM+GK
Sbjct: 245 SNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGK 304

Query: 303 KSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFML 362
           K+NL  LISKLW+VGVT+VKTTEFVQGQTSRWGLAWSFMP ARKII+  V  KSVLSFML
Sbjct: 305 KANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFML 364

Query: 363 EGLLPRVNAMSILQSIEHFLST 384
           E +  + +A+ +LQS+E F  +
Sbjct: 365 ECIKRQYSAVDVLQSVEEFFKS 376