MC04g0366 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0366 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 3.3e-263
Identity = 509/903 (56.37%), Postives = 635/903 (70.32%), Query Frame = 0

Query: 58  DPSDRTEVILKIDDGGSAAVSRPVEGNG-GKVWRETNYDFWNNDGKGE-NGASRASGASR 117
           D +DR EVI+KID  G    +  V G   GK+WR+ +YDFW  DG+G  N    A+    
Sbjct: 4   DAADRREVIVKID--GENGNNNGVSGETVGKIWRDGSYDFW-TDGEGNLNKGHNAAAVDS 63

Query: 118 VSGARASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQ 177
              A  +    ++EGFEF +RG     EDPP KLIG+FLHKQ+  GE  LDMDL M+EL 
Sbjct: 64  DRSAATTGEQQKDEGFEF-RRG-----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL- 123

Query: 178 QDRIIPPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQ 237
           Q R + P++ESP   T +                D + R  RDSR               
Sbjct: 124 QSRGLTPVSESPRVSTKR----------------DPVGR--RDSR--------------- 183

Query: 238 SHHERHGSPTISGVQNDCAAEAARCT-SNLSFQRELSFQRTSHLLRAKTKSRLMD----- 297
                      S   N+   E  +C+ +N   QR      +S LL+ +T+SRL D     
Sbjct: 184 -----------SNTNNNDDGEVVKCSGNNAPIQRS-----SSTLLKMRTRSRLSDPPTPQ 243

Query: 298 -PPEEPALLSGLIPKSGPLRSGFLGK-----GEEEDDDPFLEEDLPEDFKRSDFSVLTLL 357
            PP+   + SG IPKSG ++SGF GK     GEEE+DDPF  EDLPE++++   S+  +L
Sbjct: 244 LPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVL 303

Query: 358 QWISLVLITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIE 417
           +W+SL+LI    VCTL IP LR   LWEL +WKWE MVL+LICGRLVS W ++I+VF IE
Sbjct: 304 EWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIE 363

Query: 418 RNFLLRKRLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSL 477
           RNFLLRKR+LYFVYGVRK VQNC+WLGLVL+AW  LF+++V    N   L+ V ++ V L
Sbjct: 364 RNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCL 423

Query: 478 LVSTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEER 537
           LV  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPLIEI+KNEE EER
Sbjct: 424 LVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEER 483

Query: 538 LADEVQKLQNAGVSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGND 597
           ++ EV+K QN G  +     A  S +K+G+      +   SH  S+ G          N 
Sbjct: 484 ISVEVKKFQNPG-GVEIQSGAQKSPMKTGK------SPFLSHVLSNGGGGG-----GENK 543

Query: 598 GITIDHLHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKA 657
           GITID LHKL+PKNVSAW MKRL+N++R GS++TLDEQ+  P L+D+   +IRSE EAK 
Sbjct: 544 GITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKL 603

Query: 658 AAKKIFQNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAF 717
           AA+KIF NVA+   K+IY  D+MRF+ +DE L+T+SLFEGA+E+ RISKSSLKNWVVNAF
Sbjct: 604 AARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAF 663

Query: 718 RERRSLALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAF 777
           RERR+LALTLNDTKTAV+RLH MVN++ GI+IL++WLI+LGI S+KF   +SSQ+VVVAF
Sbjct: 664 RERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAF 723

Query: 778 IFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNS 837
           IFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+DN KV+ PNS
Sbjct: 724 IFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNS 783

Query: 838 VLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFK 897
           +L TK I N+YRSPDMG+ +EF IHI TPAE I  +KQRI SYIEG K HW P+PM VFK
Sbjct: 784 LLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFK 835

Query: 898 DVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSL 947
           D+E LN +R+AVW +HRMNHQD GE+WARRS LVE++ K+C+ELDI+YRL P+DINV +L
Sbjct: 844 DMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNL 835

BLAST of MC04g0366 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 855.5 bits (2209), Expect = 5.7e-247
Identity = 492/968 (50.83%), Postives = 655/968 (67.67%), Query Frame = 0

Query: 5   KKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPSDRTE 64
           + SFKS+ S+K   +I + G  +E S + LPIL DH P     H G    +    S R+ 
Sbjct: 4   RNSFKSHSSYK---QIRSPGDQSEPSPEHLPILHDHHP----DHSGMVVDDQKPDSTRS- 63

Query: 65  VILKIDDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGARASDN 124
               +DDG +A    PVE       R+ +Y FW ++  G +    A   S       S  
Sbjct: 64  ---SLDDGRNA----PVE-------RDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRK 123

Query: 125 GDRNEG-FEFVQRGYGVEVEDPPMKLIGEFLHKQ---KLRGETTLDMDLEMEELQQDRII 184
           GDR  G F+FV     V+ E P   + GE +++Q   +   E TLD+D E +++    + 
Sbjct: 124 GDRLSGSFDFVHGKLPVD-ESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMP 183

Query: 185 PPLAESPLS-QTSKDLKVSF---------------QQDSTESSSNDSIRRRYRDSRELQD 244
            P + +  S   S++++VSF                  S  SSS+ +  R  +D  +LQ+
Sbjct: 184 TPTSTARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQE 243

Query: 245 EYKGQQSPWQQSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTK 304
           E                              E  RCTSN+SFQR     ++  + R KT+
Sbjct: 244 E------------------------------EVVRCTSNMSFQR-----KSELISRVKTR 303

Query: 305 SRLMDPP-EEPALLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLL 364
           SRL DPP EE    SG   +SG L+SG L   +EE DDP  EED+P+++KR     +TLL
Sbjct: 304 SRLQDPPREEETPYSGW--RSGQLKSGLLADIDEE-DDPLAEEDVPDEYKRGKLDAITLL 363

Query: 365 QWISLVLITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIE 424
           QW+SLV I  AL C+L I   + + +W L++WKWEV +L+LICGRLVSGWGIRI+VF IE
Sbjct: 364 QWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIE 423

Query: 425 RNFLLRKRLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSL 484
           RNFLLRKR+LYFVYGVR+ VQNC+WLGLVL+AW  LF+K+V+ ET    L YV ++LV  
Sbjct: 424 RNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCF 483

Query: 485 LVSTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEER 544
           L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LFNQYVIETLSGPP+IE+ + EE EER
Sbjct: 484 LLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEER 543

Query: 545 LADEVQKLQNAGVSIPPDL-KADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGN 604
             DE+ K+QNAG ++PPDL  A     KSGRV+              + K S  + K+  
Sbjct: 544 AQDEIFKMQNAGANLPPDLCAAAFPPGKSGRVM--------------NPKLSPIIPKSTT 603

Query: 605 D-GITIDHLHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREA 664
           D GI+++HLH+++ KN+SAWNMKRL+ +VR  S++TLDEQ+     EDESTR+IRSE+EA
Sbjct: 604 DNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEA 663

Query: 665 KAAAKKIFQNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVN 724
           KAAA+KIF+NV +R  KYIY EDLMRF+REDE ++TM LFEGA E++RISKS+LKNW+VN
Sbjct: 664 KAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVN 723

Query: 725 AFRERRSLALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVV 784
           AFRERR+LALTLNDTKTAV++LHHM+N++  I+I+++WL+LL IASSK   FVSSQ+V++
Sbjct: 724 AFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLL 783

Query: 785 AFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIP 844
           AFIFGNT KT+FE+IIFLF++HP+DVGDRCEID +Q+VVEEMNILTTVFLRYDNLK++ P
Sbjct: 784 AFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYP 843

Query: 845 NSVLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFV 904
           NS+L  K I+N+YRSPDMG+++EFC+HI TP E I+ +KQRI +YI+    +W P    +
Sbjct: 844 NSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKII 896

Query: 905 FKDVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVH 950
            KD+E+L+ +RLA+W  HR+NHQD  ERW RR+VLVE+V+K+  ELDIQ+R  P+DINV 
Sbjct: 904 VKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVR 896

BLAST of MC04g0366 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 3.4e-244
Identity = 483/922 (52.39%), Postives = 624/922 (67.68%), Query Frame = 0

Query: 58  DPSDRTEVILKI---DDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGAS 117
           D +DR + I+ I   + G   A        GG +W+E++YDFW+ + KG+N         
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGE-KGKN--------- 64

Query: 118 RVSGARASDNGDRNEGFEFVQRG----YGVEVEDPPMKLIGEFLHKQKLRG-ETTLDMDL 177
                +  D  +    F F QRG       E+ DPP KLIG+FLHKQ+  G E +LD++L
Sbjct: 65  ---DKKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVEL 124

Query: 178 EMEELQQDRIIPPLAESPLSQTSKDLKVSFQQDSTESS--SNDSIRRRYRDSRELQDEYK 237
            M ELQ +   PP   +  +   + L    +  S   +    D++RRR            
Sbjct: 125 NMAELQSN--TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR------------ 184

Query: 238 GQQSPWQQSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRL 297
                  Q+     GS    G   D  AE  +C S           +   L R KTKSRL
Sbjct: 185 -------QNRTSLGGSSDEEGRNRD-EAEVLKCGS-----------KKPMLSRNKTKSRL 244

Query: 298 MDPP-------EEPALLSGLIPKSGPLRSGFLGKG------------EEEDDDPFLEEDL 357
            DPP       ++  + SG   +SG  +SGFLGK             EEE++DPFL+EDL
Sbjct: 245 QDPPTPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDL 304

Query: 358 PEDFKRSDFSVLTLLQWISLVLITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGR 417
           PE+FKR   S    L+WISLVLI  +LVC+L I  L+  + W+L++WKWEV VL+LICGR
Sbjct: 305 PEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGR 364

Query: 418 LVSGWGIRIIVFCIERNFLLRKRLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESET 477
           LVS W +RIIVF +E+NF  RKR+LYFVYGVRK VQNC+WLGLVL+AW  LF+K+VE ET
Sbjct: 365 LVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERET 424

Query: 478 NGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 537
             T L+YV RVLV LLV+ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSG
Sbjct: 425 RSTALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSG 484

Query: 538 PPLIEIRKNEEAEERLADEVQKLQN-AGVSIPPDLKADTSSVKSGRVIGGGVNQKSSHGK 597
           PPL+EI++ EE E+++A++V+ L+  AG  +PP LKA   +VKS   +G       S G 
Sbjct: 485 PPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKA---TVKSFMKVG------KSPGL 544

Query: 598 SSHGKSSRPLTKNGND--GITIDHLHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGP 657
           +  G      +K G D  GI ID L +++ KNVSAWNMKRL+N++  G+ISTLD+ +   
Sbjct: 545 NRIG------SKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT 604

Query: 658 CLEDESTREIRSEREAKAAAKKIFQNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGAT 717
             EDE    IRSE EAK AA+KIF NV     +YIY ED +RF+ E+E  R M+LFEGA+
Sbjct: 605 TQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGAS 664

Query: 718 ESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGI 777
           ES +ISKS LKNWVV AFRERR+LALTLNDTKTAVDRLH ++NV+ GI+I+I+WL++LGI
Sbjct: 665 ESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGI 724

Query: 778 ASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNI 837
           A+++F   +SSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNI
Sbjct: 725 ATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNI 784

Query: 838 LTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIIS 897
           LTTVFLRYDN K+I PNSVL TK I N+YRSPDMG++VEFC+HIATP E I  +KQRI+S
Sbjct: 785 LTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILS 844

Query: 898 YIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQ 948
           Y++  K +W P+PM VF  +++LN +++AVWL+HRMNHQD GER+ RR +L+E+V K C+
Sbjct: 845 YVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCR 863

BLAST of MC04g0366 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 4.5e-236
Identity = 454/886 (51.24%), Postives = 608/886 (68.62%), Query Frame = 0

Query: 83  GNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGARASDNGDRNEGFEFVQRGYGVEV 142
           G+  K WRE++ +FW+ND            +S+  G    D       F+F++R      
Sbjct: 22  GDSEKFWRESSINFWHND-----------KSSKPPGGEEDDG-----SFDFMRRSSEKSE 81

Query: 143 E-DPPMKLIGEFLHKQKLRG-ETTLDMDLEMEELQQDRIIPPLAESPLSQTSKDLKVSFQ 202
           E DPP KLI +FL+KQK  G E +LDM+  M ELQ++  +PPL+ + +S ++  +     
Sbjct: 82  EPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKN-TVPPLSSTAVSGSASPVTAPVT 141

Query: 203 QDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHERHGSPTISGVQNDCAAEAARC 262
             S  + + D+IRRR            G  S  +++  +               +E  +C
Sbjct: 142 A-SYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVD--------------GSEVVKC 201

Query: 263 TSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEE--PALLSGLIPKSGPLRSGFLG----- 322
           TSN       S  RT  L++ KT+SRLMDPP    P ++SG  P+SG L  GF G     
Sbjct: 202 TSN------RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKP 261

Query: 323 --------KGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLYIPYLR 382
                   K  EE++DPF EEDLPE  ++    V  +++WI L+LI  +L+C+L IPYLR
Sbjct: 262 GTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLR 321

Query: 383 AMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVRKPVQN 442
             +LW+L +WKWEVMVL+LICGRLVS W +++ V+ +E NFL RK++LYFVYG+RKPVQN
Sbjct: 322 GKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQN 381

Query: 443 CIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVLASSFH 502
           C+WLGLVLIAW  LF+K+VE E   TVLKYV +VL+ LLV+ +IWL+KTL+VKVLASSFH
Sbjct: 382 CLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFH 441

Query: 503 VSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPPDLKAD 562
           +STYFDRIQESLF QYVIETLSGPP IEI      EE++A++V+  +  G  + P L   
Sbjct: 442 MSTYFDRIQESLFTQYVIETLSGPPRIEIHIE---EEKVANDVKTFEIVGRKLSP-LGPK 501

Query: 563 TSSVKSGRVIGGGVNQKSSH--GKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSAWNM 622
             S      +G G  QKS    GKS     S    + G +GI IDHL +++ KNVSAW M
Sbjct: 502 AVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKM 561

Query: 623 KRLLNMVRYGSISTLDEQIPGPCL-EDESTREIRSEREAKAAAKKIFQNVARRELKYIYP 682
           K+L+N+++ G++STLDEQI      ED+   +IRSE EAK AA+KIFQNVA    +YIY 
Sbjct: 562 KKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYM 621

Query: 683 EDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAVDR 742
           ED MRF+ EDE  R M LFEGA+E  +ISKS LKNWVVNAFRERR+LALTLNDTKTAV+R
Sbjct: 622 EDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNR 681

Query: 743 LHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLFVM 802
           LH +V+V+  I+ILI+WL++LGIA++KF   +SSQ+++V F+FGN+CKTIFEA+IF+FVM
Sbjct: 682 LHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVM 741

Query: 803 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 862
           HPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ PNS+L TK I N+YRSPDM ++
Sbjct: 742 HPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDA 801

Query: 863 VEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHRMN 922
           +EF +HIATP E    ++QRI+SY++  K HW PSPM VF+D+  LN +++A+W +H+MN
Sbjct: 802 IEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMN 861

Query: 923 HQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAP 949
           HQ+ GER+ RR  L+E++ ++C+ELDI+YRL P++INV SLP++ P
Sbjct: 862 HQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATP 865

BLAST of MC04g0366 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 2.0e-212
Identity = 406/773 (52.52%), Postives = 552/773 (71.41%), Query Frame = 0

Query: 195 LKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHERHGSPTISGVQNDCA 254
           L V+ + + TE  SN++       S+E +  +K   S          G+  +SG    C 
Sbjct: 83  LDVNEETEETEDVSNNN---NLSGSKETRVFFKINSS----------GTNNMSGSVRSCT 142

Query: 255 AEA--ARCTSNLSFQREL-----------SFQRTSHLLRAKTKSRLMDPP-EEPALLSGL 314
           +    +  T  L+ +++L           S ++T  + RAK +SRL+DPP EE    S  
Sbjct: 143 SSTSFSSATMRLNLEQQLEDEGEVVVRCSSVRKTELVSRAKARSRLIDPPQEEEQQYSSW 202

Query: 315 IPKSGPLRSGFLGKGE---EEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVC 374
           I  S  LRSG LG+     EE+DD   EED+P ++++     +TLLQW+SL+ +  ALV 
Sbjct: 203 IGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVL 262

Query: 375 TLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVY 434
           +L +   R  +LW L++WKWEV++L+LICGRLVSG GIRIIVF IERNFLLRKR+LYFVY
Sbjct: 263 SLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVY 322

Query: 435 GVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMV 494
           GV+  VQNC+WLGLVL+AW  LF+K+VE ET   VL  + ++LV  L+ST++WL+KTL+V
Sbjct: 323 GVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVV 382

Query: 495 KVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVS 554
           KVLASSFHVSTYFDRIQE+LF+ Y+IETLSGPP++E+ + EE E+R  DE+ K+Q  G  
Sbjct: 383 KVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGAD 442

Query: 555 IPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGND-GITIDHLHKLSPK 614
           + P+L                  Q+ S G + + K S  + K G+D GIT+D LHK++ K
Sbjct: 443 LSPEL------------CSAAFPQEKS-GSTMNMKFSPIIPKTGSDNGITMDDLHKMNQK 502

Query: 615 NVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRE 674
           NVSAWNMKRL+ +VR  S+STLDEQ      EDESTR+IRSE+EAKAAA+KIF+NVA+  
Sbjct: 503 NVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDESTRQIRSEKEAKAAARKIFKNVAQPG 562

Query: 675 LKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDT 734
            K+IY EDLMRF+R DE ++TM LFEGA  +++I+KS+LKNW+VNAFRERR+LALTLNDT
Sbjct: 563 TKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDT 622

Query: 735 KTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAI 794
           KTAV++LHHM++ +  I+I+++WLILL IA+SK+  F++SQ+V++AF+FGN+ KT+FE+I
Sbjct: 623 KTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESI 682

Query: 795 IFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRS 854
           IFLF++HP+DVGDR  ID ++MVVEEMNILTTVFLR DNLK++ PN +L  K IHN+ RS
Sbjct: 683 IFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRS 742

Query: 855 PDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVW 914
           PDMG+ V  C+HI TP E IA +KQRI SYI+    +W P    + KDVE+LN +R+A+W
Sbjct: 743 PDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIW 802

Query: 915 LSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPS 950
           L H++NHQ+ GER+ RR++L+E+V+K+  ELDIQYR  P+DINV ++P+   S
Sbjct: 803 LCHKINHQNMGERFTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSS 829

BLAST of MC04g0366 vs. NCBI nr
Match: XP_022135714.1 (mechanosensitive ion channel protein 6-like [Momordica charantia])

HSP 1 Score: 1847 bits (4785), Expect = 0.0
Identity = 955/955 (100.00%), Postives = 955/955 (100.00%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60
           MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS
Sbjct: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60

Query: 61  DRTEVILKIDDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGAR 120
           DRTEVILKIDDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGAR
Sbjct: 61  DRTEVILKIDDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGAR 120

Query: 121 ASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII 180
           ASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII
Sbjct: 121 ASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII 180

Query: 181 PPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHER 240
           PPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHER
Sbjct: 181 PPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHER 240

Query: 241 HGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSGL 300
           HGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSGL
Sbjct: 241 HGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSGL 300

Query: 301 IPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLY 360
           IPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLY
Sbjct: 301 IPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLY 360

Query: 361 IPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVR 420
           IPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVR
Sbjct: 361 IPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVR 420

Query: 421 KPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVL 480
           KPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVL
Sbjct: 421 KPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPP 540

Query: 541 DLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSA 600
           DLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSA
Sbjct: 541 DLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELKYI 660
           WNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELKYI
Sbjct: 601 WNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELKYI 660

Query: 661 YPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAV 720
           YPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAV
Sbjct: 661 YPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAV 720

Query: 721 DRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHR 900
           ESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHR
Sbjct: 841 ESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTST 955
           MNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTST
Sbjct: 901 MNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTST 955

BLAST of MC04g0366 vs. NCBI nr
Match: XP_008448113.1 (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1471 bits (3808), Expect = 0.0
Identity = 776/959 (80.92%), Postives = 847/959 (88.32%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60
           MDT+KKSFK NVSFKHTRKISAGG  +EI+H+ELPILL+H+    H       +ND DPS
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHL------VNDSDPS 60

Query: 61  DRTEVILKIDDGGSAAVSRPVEG---NGGKVWRETNYDFWNNDGKGENGASRASGASRVS 120
           DRTEVILKIDDGGS+AVSR ++    NGGKVWRE+ YDFWNND   E G   +  ASRV 
Sbjct: 61  DRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNND---ETGIGES--ASRVR 120

Query: 121 GARASDNGDR-NEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180
           GAR SD+GD  NEGF+FVQ GYG+E  DPP KLIG+FLHKQK+RGETTLDMDLEMEEL+ 
Sbjct: 121 GARMSDSGDDGNEGFQFVQTGYGME--DPPTKLIGDFLHKQKIRGETTLDMDLEMEELKP 180

Query: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQ 240
           +RIIPPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +L++E+KG+Q PWQQ
Sbjct: 181 NRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQ 240

Query: 241 SHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPA 300
           SHHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL DPP EP 
Sbjct: 241 SHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPD 300

Query: 301 LLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGAL 360
            LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP+DFKR +F+ LT+LQW SL+LIT A 
Sbjct: 301 RLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAF 360

Query: 361 VCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYF 420
           +CTL +PYLR  SLWEL+IWKWEVM+LILICGRLVSGWGIRI VF IERNFLLRKR+LYF
Sbjct: 361 ICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYF 420

Query: 421 VYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTL 480
           VYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKTL
Sbjct: 421 VYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTL 480

Query: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAG 540
           MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNAG
Sbjct: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG 540

Query: 541 VSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSP 600
           ++IPPDLKA  +S+KSGR I      KS   KSS  K SR LTKNGNDGITIDHLHKLSP
Sbjct: 541 LTIPPDLKATFASIKSGRAISSERTHKSFCAKSS--KFSRALTKNGNDGITIDHLHKLSP 600

Query: 601 KNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARR 660
           KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVARR
Sbjct: 601 KNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARR 660

Query: 661 ELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLND 720
             KYIY +DLMRFMREDEVL+T SLFEGA E+RRISKS LKNWVVN FRERR+LALTLND
Sbjct: 661 GYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLND 720

Query: 721 TKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEA 780
           TKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIFEA
Sbjct: 721 TKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEA 780

Query: 781 IIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYR 840
           IIFLFVMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYR
Sbjct: 781 IIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYR 840

Query: 841 SPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAV 900
           SPDMGESVEF +HIATPAE I  MKQRIISYIEGNK HW P+PM VFKD++ LN+L+LAV
Sbjct: 841 SPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAV 900

Query: 901 WLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTS 954
           WLSHRMNHQDS ERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 901 WLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPS 944

BLAST of MC04g0366 vs. NCBI nr
Match: KAA0040324.1 (mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa] >TYK23381.1 mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1469 bits (3802), Expect = 0.0
Identity = 775/959 (80.81%), Postives = 846/959 (88.22%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60
           MDT+KKSFK NVSFKHTRKISAGG  +EI+H+ELPILL+H+    H       +ND DPS
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHL------VNDSDPS 60

Query: 61  DRTEVILKIDDGGSAAVSRPVEG---NGGKVWRETNYDFWNNDGKGENGASRASGASRVS 120
           DRTEVILKIDD GS+AVSR ++    NGGKVWRE+ YDFWNND   E G   +  ASRV 
Sbjct: 61  DRTEVILKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNND---ETGIGES--ASRVR 120

Query: 121 GARASDNGDR-NEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180
           GAR SD+GD  NEGF+FVQ GYG+E  DPP KLIG+FLHKQK+RGETTLDMDLEMEEL+ 
Sbjct: 121 GARMSDSGDDGNEGFQFVQTGYGME--DPPTKLIGDFLHKQKIRGETTLDMDLEMEELKP 180

Query: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQ 240
           +RIIPPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +L++E+KG+Q PWQQ
Sbjct: 181 NRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQ 240

Query: 241 SHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPA 300
           SHHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL DPP EP 
Sbjct: 241 SHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPD 300

Query: 301 LLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGAL 360
            LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP+DFKR +F+ LT+LQW SL+LIT A 
Sbjct: 301 RLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAF 360

Query: 361 VCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYF 420
           +CTL +PYLR  SLWEL+IWKWEVM+LILICGRLVSGWGIRI VF IERNFLLRKR+LYF
Sbjct: 361 ICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYF 420

Query: 421 VYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTL 480
           VYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKTL
Sbjct: 421 VYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTL 480

Query: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAG 540
           MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNAG
Sbjct: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG 540

Query: 541 VSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSP 600
           ++IPPDLKA  +S+KSGR I      KS   KSS  K SR LTKNGNDGITIDHLHKLSP
Sbjct: 541 LTIPPDLKATFASIKSGRAISSERTHKSFCAKSS--KFSRALTKNGNDGITIDHLHKLSP 600

Query: 601 KNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARR 660
           KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVARR
Sbjct: 601 KNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARR 660

Query: 661 ELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLND 720
             KYIY +DLMRFMREDEVL+T SLFEGA E+RRISKS LKNWVVN FRERR+LALTLND
Sbjct: 661 GYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLND 720

Query: 721 TKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEA 780
           TKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIFEA
Sbjct: 721 TKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEA 780

Query: 781 IIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYR 840
           IIFLFVMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYR
Sbjct: 781 IIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYR 840

Query: 841 SPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAV 900
           SPDMGESVEF +HIATPAE I  MKQRIISYIEGNK HW P+PM VFKD++ LN+L+LAV
Sbjct: 841 SPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAV 900

Query: 901 WLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTS 954
           WLSHRMNHQDS ERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 901 WLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPS 944

BLAST of MC04g0366 vs. NCBI nr
Match: XP_038887578.1 (mechanosensitive ion channel protein 6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1463 bits (3787), Expect = 0.0
Identity = 779/963 (80.89%), Postives = 851/963 (88.37%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRH-RGRRSMNDCDP 60
           MDT+KKSFK NVSFKHTRKISAGG G+EI+H+ELPILL+HEPA   R  R R  +ND D 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPADHRRRLRDRHPVNDSDS 60

Query: 61  SDRTEVILKIDDGGSAAVSRPVEG---NGGKVWRETNYDFWNND--GKGENGASRASGAS 120
           SD TEVILKIDDGGS+ VSR ++    NGGKVWRE+ Y FWNN+  G GE+       AS
Sbjct: 61  SDGTEVILKIDDGGSSTVSRSLDSVGSNGGKVWRESRYGFWNNNAIGIGES-------AS 120

Query: 121 RVSGARASDNG-DRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEE 180
           RVSGAR SD+G DRNEGFEFVQ GYG+E  DPP KLIGEFLHKQK+RGETTLDMDLEMEE
Sbjct: 121 RVSGARMSDSGVDRNEGFEFVQPGYGME--DPPTKLIGEFLHKQKIRGETTLDMDLEMEE 180

Query: 181 LQQDRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSP 240
           L+ DR + PL ESPL+QTSKDLKVSFQQDSTE SSND S+RRR RDSR+L +E+KG Q P
Sbjct: 181 LKPDRSMTPLPESPLNQTSKDLKVSFQQDSTEISSNDQSVRRRCRDSRDLPEEFKGGQPP 240

Query: 241 WQQSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPE 300
           WQQSHHER GSPTISGVQN+ +AEA R  SNLSF  ELSFQR S+LLRAKTKSRL+D P 
Sbjct: 241 WQQSHHERLGSPTISGVQNESSAEAMRSASNLSFHSELSFQRKSNLLRAKTKSRLIDSPA 300

Query: 301 EPALLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLIT 360
           EP  LSGLIPKSG LRSGFLGK +++DDDPFLEEDLP+DFKR +F+ LT+LQWISL+LIT
Sbjct: 301 EPDRLSGLIPKSGQLRSGFLGKIDDDDDDPFLEEDLPDDFKRGNFNALTVLQWISLILIT 360

Query: 361 GALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRL 420
            ALVCTL IPYLR  SLWEL+IWKWEVM+LILICGRLVSGWGI+I+VF IERNFLLRK++
Sbjct: 361 AALVCTLSIPYLREKSLWELDIWKWEVMILILICGRLVSGWGIKIVVFFIERNFLLRKKV 420

Query: 421 LYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLV 480
           LYFVYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YVRRVLV LL+ST +WLV
Sbjct: 421 LYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNASILNYVRRVLVCLLISTFLWLV 480

Query: 481 KTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQ 540
           KTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEI KNEE EER+ADEVQKLQ
Sbjct: 481 KTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEILKNEEEEERVADEVQKLQ 540

Query: 541 NAGVSIPPDLKADT-SSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLH 600
           NAG++IP DLKA T SSVKSGRVIG G   KS   KSS  K SR LTKNGNDGITIDHLH
Sbjct: 541 NAGINIPSDLKAATFSSVKSGRVIGSGRTHKSFCAKSS--KLSRALTKNGNDGITIDHLH 600

Query: 601 KLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQN 660
           KLSPKNVSAWNMKRLLN+VRYGSISTLDEQI GP  +DEST EI+SEREAKAAAKKIFQN
Sbjct: 601 KLSPKNVSAWNMKRLLNIVRYGSISTLDEQIQGPGRDDESTTEIKSEREAKAAAKKIFQN 660

Query: 661 VARRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLAL 720
           VARR  KYIY +DLMRFMREDEV +TMSLFEGA E+RRISKS+LKNWVVNAFRERR+LAL
Sbjct: 661 VARRGYKYIYLDDLMRFMREDEVFKTMSLFEGAAENRRISKSALKNWVVNAFRERRALAL 720

Query: 721 TLNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKT 780
           TLNDTKTAVD+LHHMVNVIF ILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKT
Sbjct: 721 TLNDTKTAVDKLHHMVNVIFSILILILWLIVLGIASSKFFVFLSSQIVVVAFIFGNTCKT 780

Query: 781 IFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIH 840
           IFEAIIFLFVMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIH
Sbjct: 781 IFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIH 840

Query: 841 NFYRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRL 900
           NFYRSP+MGESVEF +HIATPAE I  MKQRIISYIEGNK HW PSPM VF D+++LN+L
Sbjct: 841 NFYRSPEMGESVEFYVHIATPAEKITAMKQRIISYIEGNKEHWCPSPMVVFMDIDDLNKL 900

Query: 901 RLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMG 954
           +LAVWLSHRMNHQD+GERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+G
Sbjct: 901 KLAVWLSHRMNHQDAGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIG 952

BLAST of MC04g0366 vs. NCBI nr
Match: XP_004144925.1 (mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus] >KGN43281.1 hypothetical protein Csa_020565 [Cucumis sativus])

HSP 1 Score: 1432 bits (3707), Expect = 0.0
Identity = 766/965 (79.38%), Postives = 839/965 (86.94%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRH-RGRRSMNDCDP 60
           MDT+KKSFK NVSFKHTRKISAGG G+EI+H+ELPILL+H+     R  R R  +ND D 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDDGGSA-AVSRPVEG---NGGKVWRETNYDFWNND--GKGENGASRASGA 120
           SDRTEVILKID GGS+ AVSR ++    NGG VWRE+ YDFWNND  G GE+        
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGES-------- 120

Query: 121 SRVSGARASDNG--DRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEM 180
                AR  DN   DRNEGFEFVQ GYG+E  DPP KLIG+FL KQK+ GETTLDMDLEM
Sbjct: 121 -----ARMGDNSGVDRNEGFEFVQTGYGME--DPPTKLIGDFLCKQKIEGETTLDMDLEM 180

Query: 181 EELQQDRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQ 240
           EEL+ +RI+PPLAESPLSQTSKDLKVSFQ DSTE SSND SIRRR RDS +L++E KG Q
Sbjct: 181 EELKPNRIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQ 240

Query: 241 SPWQQSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDP 300
           SP QQ HHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL+DP
Sbjct: 241 SPRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDP 300

Query: 301 PEEPALLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVL 360
           P EP  LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP++FKR +FS LT+LQW+SL++
Sbjct: 301 PAEPDRLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLII 360

Query: 361 ITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRK 420
           IT AL+CTL +PYLR  SLWEL+IWKWEVM+ ILICGRLVSGWGIRI VF IERNFLLRK
Sbjct: 361 ITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRK 420

Query: 421 RLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIW 480
           R+LYFVYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIW
Sbjct: 421 RVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIW 480

Query: 481 LVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQK 540
           LVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQK
Sbjct: 481 LVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQK 540

Query: 541 LQNAGVSIPPDLKADT-SSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDH 600
           LQNAG+ IPPDLKA T +S+KSGR IG G   KS   KS   K SR LTKN NDGITIDH
Sbjct: 541 LQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSC--KLSRALTKNRNDGITIDH 600

Query: 601 LHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIF 660
           LHKLS KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIF
Sbjct: 601 LHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIF 660

Query: 661 QNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSL 720
           QNVA R  KYIY +DL+RFMREDEVL+TMSLFEGA E++RISKS+LKNWVVNAFRERR+L
Sbjct: 661 QNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRAL 720

Query: 721 ALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTC 780
           ALTLNDTKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTC
Sbjct: 721 ALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTC 780

Query: 781 KTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKL 840
           KTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKL
Sbjct: 781 KTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKL 840

Query: 841 IHNFYRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELN 900
           IHNFYRSPDMGES+EF +HIATPAE I  MK RIISYIEGNK HW P+PM VFKD++ LN
Sbjct: 841 IHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLN 900

Query: 901 RLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPS 954
           +++LAVWLSHRMNHQDSGERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS
Sbjct: 901 KVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPS 948

BLAST of MC04g0366 vs. ExPASy TrEMBL
Match: A0A6J1C288 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111007608 PE=3 SV=1)

HSP 1 Score: 1847 bits (4785), Expect = 0.0
Identity = 955/955 (100.00%), Postives = 955/955 (100.00%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60
           MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS
Sbjct: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60

Query: 61  DRTEVILKIDDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGAR 120
           DRTEVILKIDDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGAR
Sbjct: 61  DRTEVILKIDDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGAR 120

Query: 121 ASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII 180
           ASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII
Sbjct: 121 ASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII 180

Query: 181 PPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHER 240
           PPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHER
Sbjct: 181 PPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHER 240

Query: 241 HGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSGL 300
           HGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSGL
Sbjct: 241 HGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSGL 300

Query: 301 IPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLY 360
           IPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLY
Sbjct: 301 IPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLY 360

Query: 361 IPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVR 420
           IPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVR
Sbjct: 361 IPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVR 420

Query: 421 KPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVL 480
           KPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVL
Sbjct: 421 KPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPP 540

Query: 541 DLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSA 600
           DLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSA
Sbjct: 541 DLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELKYI 660
           WNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELKYI
Sbjct: 601 WNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELKYI 660

Query: 661 YPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAV 720
           YPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAV
Sbjct: 661 YPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAV 720

Query: 721 DRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHR 900
           ESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHR
Sbjct: 841 ESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTST 955
           MNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTST
Sbjct: 901 MNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTST 955

BLAST of MC04g0366 vs. ExPASy TrEMBL
Match: A0A1S3BID3 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490400 PE=3 SV=1)

HSP 1 Score: 1471 bits (3808), Expect = 0.0
Identity = 776/959 (80.92%), Postives = 847/959 (88.32%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60
           MDT+KKSFK NVSFKHTRKISAGG  +EI+H+ELPILL+H+    H       +ND DPS
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHL------VNDSDPS 60

Query: 61  DRTEVILKIDDGGSAAVSRPVEG---NGGKVWRETNYDFWNNDGKGENGASRASGASRVS 120
           DRTEVILKIDDGGS+AVSR ++    NGGKVWRE+ YDFWNND   E G   +  ASRV 
Sbjct: 61  DRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNND---ETGIGES--ASRVR 120

Query: 121 GARASDNGDR-NEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180
           GAR SD+GD  NEGF+FVQ GYG+E  DPP KLIG+FLHKQK+RGETTLDMDLEMEEL+ 
Sbjct: 121 GARMSDSGDDGNEGFQFVQTGYGME--DPPTKLIGDFLHKQKIRGETTLDMDLEMEELKP 180

Query: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQ 240
           +RIIPPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +L++E+KG+Q PWQQ
Sbjct: 181 NRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQ 240

Query: 241 SHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPA 300
           SHHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL DPP EP 
Sbjct: 241 SHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPD 300

Query: 301 LLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGAL 360
            LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP+DFKR +F+ LT+LQW SL+LIT A 
Sbjct: 301 RLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAF 360

Query: 361 VCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYF 420
           +CTL +PYLR  SLWEL+IWKWEVM+LILICGRLVSGWGIRI VF IERNFLLRKR+LYF
Sbjct: 361 ICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYF 420

Query: 421 VYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTL 480
           VYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKTL
Sbjct: 421 VYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTL 480

Query: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAG 540
           MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNAG
Sbjct: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG 540

Query: 541 VSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSP 600
           ++IPPDLKA  +S+KSGR I      KS   KSS  K SR LTKNGNDGITIDHLHKLSP
Sbjct: 541 LTIPPDLKATFASIKSGRAISSERTHKSFCAKSS--KFSRALTKNGNDGITIDHLHKLSP 600

Query: 601 KNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARR 660
           KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVARR
Sbjct: 601 KNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARR 660

Query: 661 ELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLND 720
             KYIY +DLMRFMREDEVL+T SLFEGA E+RRISKS LKNWVVN FRERR+LALTLND
Sbjct: 661 GYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLND 720

Query: 721 TKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEA 780
           TKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIFEA
Sbjct: 721 TKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEA 780

Query: 781 IIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYR 840
           IIFLFVMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYR
Sbjct: 781 IIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYR 840

Query: 841 SPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAV 900
           SPDMGESVEF +HIATPAE I  MKQRIISYIEGNK HW P+PM VFKD++ LN+L+LAV
Sbjct: 841 SPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAV 900

Query: 901 WLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTS 954
           WLSHRMNHQDS ERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 901 WLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPS 944

BLAST of MC04g0366 vs. ExPASy TrEMBL
Match: A0A5D3DI81 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004470 PE=3 SV=1)

HSP 1 Score: 1469 bits (3802), Expect = 0.0
Identity = 775/959 (80.81%), Postives = 846/959 (88.22%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60
           MDT+KKSFK NVSFKHTRKISAGG  +EI+H+ELPILL+H+    H       +ND DPS
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHL------VNDSDPS 60

Query: 61  DRTEVILKIDDGGSAAVSRPVEG---NGGKVWRETNYDFWNNDGKGENGASRASGASRVS 120
           DRTEVILKIDD GS+AVSR ++    NGGKVWRE+ YDFWNND   E G   +  ASRV 
Sbjct: 61  DRTEVILKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNND---ETGIGES--ASRVR 120

Query: 121 GARASDNGDR-NEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180
           GAR SD+GD  NEGF+FVQ GYG+E  DPP KLIG+FLHKQK+RGETTLDMDLEMEEL+ 
Sbjct: 121 GARMSDSGDDGNEGFQFVQTGYGME--DPPTKLIGDFLHKQKIRGETTLDMDLEMEELKP 180

Query: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQ 240
           +RIIPPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +L++E+KG+Q PWQQ
Sbjct: 181 NRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQ 240

Query: 241 SHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPA 300
           SHHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL DPP EP 
Sbjct: 241 SHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPD 300

Query: 301 LLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGAL 360
            LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP+DFKR +F+ LT+LQW SL+LIT A 
Sbjct: 301 RLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAF 360

Query: 361 VCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYF 420
           +CTL +PYLR  SLWEL+IWKWEVM+LILICGRLVSGWGIRI VF IERNFLLRKR+LYF
Sbjct: 361 ICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYF 420

Query: 421 VYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTL 480
           VYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKTL
Sbjct: 421 VYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTL 480

Query: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAG 540
           MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNAG
Sbjct: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG 540

Query: 541 VSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSP 600
           ++IPPDLKA  +S+KSGR I      KS   KSS  K SR LTKNGNDGITIDHLHKLSP
Sbjct: 541 LTIPPDLKATFASIKSGRAISSERTHKSFCAKSS--KFSRALTKNGNDGITIDHLHKLSP 600

Query: 601 KNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARR 660
           KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVARR
Sbjct: 601 KNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARR 660

Query: 661 ELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLND 720
             KYIY +DLMRFMREDEVL+T SLFEGA E+RRISKS LKNWVVN FRERR+LALTLND
Sbjct: 661 GYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLND 720

Query: 721 TKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEA 780
           TKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIFEA
Sbjct: 721 TKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEA 780

Query: 781 IIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYR 840
           IIFLFVMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYR
Sbjct: 781 IIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYR 840

Query: 841 SPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAV 900
           SPDMGESVEF +HIATPAE I  MKQRIISYIEGNK HW P+PM VFKD++ LN+L+LAV
Sbjct: 841 SPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAV 900

Query: 901 WLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTS 954
           WLSHRMNHQDS ERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 901 WLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPS 944

BLAST of MC04g0366 vs. ExPASy TrEMBL
Match: A0A0A0K619 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_7G017150 PE=3 SV=1)

HSP 1 Score: 1432 bits (3707), Expect = 0.0
Identity = 766/965 (79.38%), Postives = 839/965 (86.94%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRH-RGRRSMNDCDP 60
           MDT+KKSFK NVSFKHTRKISAGG G+EI+H+ELPILL+H+     R  R R  +ND D 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDDGGSA-AVSRPVEG---NGGKVWRETNYDFWNND--GKGENGASRASGA 120
           SDRTEVILKID GGS+ AVSR ++    NGG VWRE+ YDFWNND  G GE+        
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGES-------- 120

Query: 121 SRVSGARASDNG--DRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEM 180
                AR  DN   DRNEGFEFVQ GYG+E  DPP KLIG+FL KQK+ GETTLDMDLEM
Sbjct: 121 -----ARMGDNSGVDRNEGFEFVQTGYGME--DPPTKLIGDFLCKQKIEGETTLDMDLEM 180

Query: 181 EELQQDRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQ 240
           EEL+ +RI+PPLAESPLSQTSKDLKVSFQ DSTE SSND SIRRR RDS +L++E KG Q
Sbjct: 181 EELKPNRIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQ 240

Query: 241 SPWQQSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDP 300
           SP QQ HHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL+DP
Sbjct: 241 SPRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDP 300

Query: 301 PEEPALLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVL 360
           P EP  LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP++FKR +FS LT+LQW+SL++
Sbjct: 301 PAEPDRLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLII 360

Query: 361 ITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRK 420
           IT AL+CTL +PYLR  SLWEL+IWKWEVM+ ILICGRLVSGWGIRI VF IERNFLLRK
Sbjct: 361 ITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRK 420

Query: 421 RLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIW 480
           R+LYFVYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIW
Sbjct: 421 RVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIW 480

Query: 481 LVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQK 540
           LVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQK
Sbjct: 481 LVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQK 540

Query: 541 LQNAGVSIPPDLKADT-SSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDH 600
           LQNAG+ IPPDLKA T +S+KSGR IG G   KS   KS   K SR LTKN NDGITIDH
Sbjct: 541 LQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSC--KLSRALTKNRNDGITIDH 600

Query: 601 LHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIF 660
           LHKLS KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIF
Sbjct: 601 LHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIF 660

Query: 661 QNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSL 720
           QNVA R  KYIY +DL+RFMREDEVL+TMSLFEGA E++RISKS+LKNWVVNAFRERR+L
Sbjct: 661 QNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRAL 720

Query: 721 ALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTC 780
           ALTLNDTKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTC
Sbjct: 721 ALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTC 780

Query: 781 KTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKL 840
           KTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKL
Sbjct: 781 KTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKL 840

Query: 841 IHNFYRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELN 900
           IHNFYRSPDMGES+EF +HIATPAE I  MK RIISYIEGNK HW P+PM VFKD++ LN
Sbjct: 841 IHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLN 900

Query: 901 RLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPS 954
           +++LAVWLSHRMNHQDSGERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS
Sbjct: 901 KVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPS 948

BLAST of MC04g0366 vs. ExPASy TrEMBL
Match: A0A6J1EJZ4 (mechanosensitive ion channel protein 6-like OS=Cucurbita moschata OX=3662 GN=LOC111435046 PE=4 SV=1)

HSP 1 Score: 1238 bits (3204), Expect = 0.0
Identity = 659/942 (69.96%), Postives = 776/942 (82.38%), Query Frame = 0

Query: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVH-RHRGRRSMNDCDP 60
           MD+ KKS K N  FKH+RKISAGG G+EI+ +ELPILLDH+P+  H R + RR  ND +P
Sbjct: 1   MDSFKKSLKHNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SDRTEVILKIDDGGSAAVSRPVE--GNGGKVWRETNYDFW-NNDGKGENGASRASGASRV 120
           S  +EVILK+DDGGS+AVSR  E  G GGKVWRE+ YDFW NNDG G  G+     AS  
Sbjct: 61  SGHSEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGS-----ASWA 120

Query: 121 SGARASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180
           SGARASD+GDRNEGFEFV+RG G +  DPP KLIG+FLHKQK+RGETTLD+DLEMEEL+ 
Sbjct: 121 SGARASDSGDRNEGFEFVRRGNGTD--DPPTKLIGDFLHKQKVRGETTLDLDLEMEELRL 180

Query: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQS 240
           DR   P+ +SPLS+ SKDLKVSFQ DSTE SSN+S+RRRY++ RE+Q+E K  Q  W QS
Sbjct: 181 DRTKTPMMDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQS 240

Query: 241 HHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPAL 300
           HHE HG  +ISGVQND  AEA R  SNLSFQ  LS  R SHLL+AK KSRL DPP EP  
Sbjct: 241 HHE-HGGSSISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDR 300

Query: 301 LSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALV 360
           LSGL+ KSG LRSGFLG+ ++E+DDPFLEED P+DF+R     LT+LQWISL LIT ALV
Sbjct: 301 LSGLVNKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALV 360

Query: 361 CTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFV 420
           CTL I +LR  SLWE +IWKWEVMVL+LICGRLVSGWGI I VF IERNFLLRK++LYFV
Sbjct: 361 CTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFV 420

Query: 421 YGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLM 480
           YGV++PVQNC+WLGLVLIAW LLF+KRV+++TN  +L YVR+ LV LL+ TLIWL KTLM
Sbjct: 421 YGVKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLM 480

Query: 481 VKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGV 540
           +KVLASSFHVSTYFDRIQESLFNQYVIETLSGPP IE++K+EE ++++ADEVQ+LQ+AGV
Sbjct: 481 LKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGV 540

Query: 541 SIPPDLKADT-SSVKSGRVIGGGV-NQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLS 600
            +PPDL+A   SS+KSG  +   V +Q+S  GK    K SR  TKNG++GI IDHLHKL+
Sbjct: 541 PVPPDLRAAALSSIKSGERVRSNVGSQRSLRGKDP--KLSRVSTKNGSEGINIDHLHKLT 600

Query: 601 PKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTR-EIRSEREAKAAAKKIFQNVA 660
            KNVSAWNMKRLL +VRYGSI+TLDEQI GP L+DEST  EI+SEREAKAAAKKIFQNVA
Sbjct: 601 SKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVA 660

Query: 661 RRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTL 720
           R   KYIY +DLMRFM+E+EVL+T+S FEGATE+RRISKS+LKNWVVNAFRERR+L+LTL
Sbjct: 661 RPGFKYIYLDDLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTL 720

Query: 721 NDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIF 780
           NDTKTAV+RL  +VNVIF I IL+LW+ILLGIAS K   ++SSQIV+VAFIFGNT K IF
Sbjct: 721 NDTKTAVERLGLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIF 780

Query: 781 EAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNF 840
           EAIIFLFVMHPFDVGDRCEID +QMVVEEMNILTTVFLR+DNLK+I+PNSVLATK+IHN 
Sbjct: 781 EAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNH 840

Query: 841 YRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRL 900
           YRSPDMGE +E C+HI TP E I  MKQRIIS+IE +K HW PSP+ + KD++  ++L +
Sbjct: 841 YRSPDMGEFIEICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAM 900

Query: 901 AVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLP 935
           ++WLSH MNHQD  ERWARRSV+VE+V+K+CQE DI   L+P
Sbjct: 901 SIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFDITPTLVP 932

BLAST of MC04g0366 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 909.4 bits (2349), Expect = 2.4e-264
Identity = 509/903 (56.37%), Postives = 635/903 (70.32%), Query Frame = 0

Query: 58  DPSDRTEVILKIDDGGSAAVSRPVEGNG-GKVWRETNYDFWNNDGKGE-NGASRASGASR 117
           D +DR EVI+KID  G    +  V G   GK+WR+ +YDFW  DG+G  N    A+    
Sbjct: 4   DAADRREVIVKID--GENGNNNGVSGETVGKIWRDGSYDFW-TDGEGNLNKGHNAAAVDS 63

Query: 118 VSGARASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQ 177
              A  +    ++EGFEF +RG     EDPP KLIG+FLHKQ+  GE  LDMDL M+EL 
Sbjct: 64  DRSAATTGEQQKDEGFEF-RRG-----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL- 123

Query: 178 QDRIIPPLAESPLSQTSKDLKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQ 237
           Q R + P++ESP   T +                D + R  RDSR               
Sbjct: 124 QSRGLTPVSESPRVSTKR----------------DPVGR--RDSR--------------- 183

Query: 238 SHHERHGSPTISGVQNDCAAEAARCT-SNLSFQRELSFQRTSHLLRAKTKSRLMD----- 297
                      S   N+   E  +C+ +N   QR      +S LL+ +T+SRL D     
Sbjct: 184 -----------SNTNNNDDGEVVKCSGNNAPIQRS-----SSTLLKMRTRSRLSDPPTPQ 243

Query: 298 -PPEEPALLSGLIPKSGPLRSGFLGK-----GEEEDDDPFLEEDLPEDFKRSDFSVLTLL 357
            PP+   + SG IPKSG ++SGF GK     GEEE+DDPF  EDLPE++++   S+  +L
Sbjct: 244 LPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVL 303

Query: 358 QWISLVLITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIE 417
           +W+SL+LI    VCTL IP LR   LWEL +WKWE MVL+LICGRLVS W ++I+VF IE
Sbjct: 304 EWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIE 363

Query: 418 RNFLLRKRLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSL 477
           RNFLLRKR+LYFVYGVRK VQNC+WLGLVL+AW  LF+++V    N   L+ V ++ V L
Sbjct: 364 RNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCL 423

Query: 478 LVSTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEER 537
           LV  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPLIEI+KNEE EER
Sbjct: 424 LVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEER 483

Query: 538 LADEVQKLQNAGVSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGND 597
           ++ EV+K QN G  +     A  S +K+G+      +   SH  S+ G          N 
Sbjct: 484 ISVEVKKFQNPG-GVEIQSGAQKSPMKTGK------SPFLSHVLSNGGGGG-----GENK 543

Query: 598 GITIDHLHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKA 657
           GITID LHKL+PKNVSAW MKRL+N++R GS++TLDEQ+  P L+D+   +IRSE EAK 
Sbjct: 544 GITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKL 603

Query: 658 AAKKIFQNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAF 717
           AA+KIF NVA+   K+IY  D+MRF+ +DE L+T+SLFEGA+E+ RISKSSLKNWVVNAF
Sbjct: 604 AARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAF 663

Query: 718 RERRSLALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAF 777
           RERR+LALTLNDTKTAV+RLH MVN++ GI+IL++WLI+LGI S+KF   +SSQ+VVVAF
Sbjct: 664 RERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAF 723

Query: 778 IFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNS 837
           IFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+DN KV+ PNS
Sbjct: 724 IFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNS 783

Query: 838 VLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFK 897
           +L TK I N+YRSPDMG+ +EF IHI TPAE I  +KQRI SYIEG K HW P+PM VFK
Sbjct: 784 LLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFK 835

Query: 898 DVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSL 947
           D+E LN +R+AVW +HRMNHQD GE+WARRS LVE++ K+C+ELDI+YRL P+DINV +L
Sbjct: 844 DMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNL 835

BLAST of MC04g0366 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 846.3 bits (2185), Expect = 2.4e-245
Identity = 483/922 (52.39%), Postives = 624/922 (67.68%), Query Frame = 0

Query: 58  DPSDRTEVILKI---DDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGAS 117
           D +DR + I+ I   + G   A        GG +W+E++YDFW+ + KG+N         
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGE-KGKN--------- 64

Query: 118 RVSGARASDNGDRNEGFEFVQRG----YGVEVEDPPMKLIGEFLHKQKLRG-ETTLDMDL 177
                +  D  +    F F QRG       E+ DPP KLIG+FLHKQ+  G E +LD++L
Sbjct: 65  ---DKKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVEL 124

Query: 178 EMEELQQDRIIPPLAESPLSQTSKDLKVSFQQDSTESS--SNDSIRRRYRDSRELQDEYK 237
            M ELQ +   PP   +  +   + L    +  S   +    D++RRR            
Sbjct: 125 NMAELQSN--TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR------------ 184

Query: 238 GQQSPWQQSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRL 297
                  Q+     GS    G   D  AE  +C S           +   L R KTKSRL
Sbjct: 185 -------QNRTSLGGSSDEEGRNRD-EAEVLKCGS-----------KKPMLSRNKTKSRL 244

Query: 298 MDPP-------EEPALLSGLIPKSGPLRSGFLGKG------------EEEDDDPFLEEDL 357
            DPP       ++  + SG   +SG  +SGFLGK             EEE++DPFL+EDL
Sbjct: 245 QDPPTPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDL 304

Query: 358 PEDFKRSDFSVLTLLQWISLVLITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGR 417
           PE+FKR   S    L+WISLVLI  +LVC+L I  L+  + W+L++WKWEV VL+LICGR
Sbjct: 305 PEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGR 364

Query: 418 LVSGWGIRIIVFCIERNFLLRKRLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESET 477
           LVS W +RIIVF +E+NF  RKR+LYFVYGVRK VQNC+WLGLVL+AW  LF+K+VE ET
Sbjct: 365 LVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERET 424

Query: 478 NGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 537
             T L+YV RVLV LLV+ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSG
Sbjct: 425 RSTALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSG 484

Query: 538 PPLIEIRKNEEAEERLADEVQKLQN-AGVSIPPDLKADTSSVKSGRVIGGGVNQKSSHGK 597
           PPL+EI++ EE E+++A++V+ L+  AG  +PP LKA   +VKS   +G       S G 
Sbjct: 485 PPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKA---TVKSFMKVG------KSPGL 544

Query: 598 SSHGKSSRPLTKNGND--GITIDHLHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGP 657
           +  G      +K G D  GI ID L +++ KNVSAWNMKRL+N++  G+ISTLD+ +   
Sbjct: 545 NRIG------SKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT 604

Query: 658 CLEDESTREIRSEREAKAAAKKIFQNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGAT 717
             EDE    IRSE EAK AA+KIF NV     +YIY ED +RF+ E+E  R M+LFEGA+
Sbjct: 605 TQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGAS 664

Query: 718 ESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGI 777
           ES +ISKS LKNWVV AFRERR+LALTLNDTKTAVDRLH ++NV+ GI+I+I+WL++LGI
Sbjct: 665 ESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGI 724

Query: 778 ASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNI 837
           A+++F   +SSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNI
Sbjct: 725 ATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNI 784

Query: 838 LTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIIS 897
           LTTVFLRYDN K+I PNSVL TK I N+YRSPDMG++VEFC+HIATP E I  +KQRI+S
Sbjct: 785 LTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILS 844

Query: 898 YIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQ 948
           Y++  K +W P+PM VF  +++LN +++AVWL+HRMNHQD GER+ RR +L+E+V K C+
Sbjct: 845 YVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCR 863

BLAST of MC04g0366 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 819.3 bits (2115), Expect = 3.2e-237
Identity = 454/886 (51.24%), Postives = 608/886 (68.62%), Query Frame = 0

Query: 83  GNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGARASDNGDRNEGFEFVQRGYGVEV 142
           G+  K WRE++ +FW+ND            +S+  G    D       F+F++R      
Sbjct: 22  GDSEKFWRESSINFWHND-----------KSSKPPGGEEDDG-----SFDFMRRSSEKSE 81

Query: 143 E-DPPMKLIGEFLHKQKLRG-ETTLDMDLEMEELQQDRIIPPLAESPLSQTSKDLKVSFQ 202
           E DPP KLI +FL+KQK  G E +LDM+  M ELQ++  +PPL+ + +S ++  +     
Sbjct: 82  EPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKN-TVPPLSSTAVSGSASPVTAPVT 141

Query: 203 QDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHERHGSPTISGVQNDCAAEAARC 262
             S  + + D+IRRR            G  S  +++  +               +E  +C
Sbjct: 142 A-SYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVD--------------GSEVVKC 201

Query: 263 TSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEE--PALLSGLIPKSGPLRSGFLG----- 322
           TSN       S  RT  L++ KT+SRLMDPP    P ++SG  P+SG L  GF G     
Sbjct: 202 TSN------RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKP 261

Query: 323 --------KGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTLYIPYLR 382
                   K  EE++DPF EEDLPE  ++    V  +++WI L+LI  +L+C+L IPYLR
Sbjct: 262 GTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLR 321

Query: 383 AMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGVRKPVQN 442
             +LW+L +WKWEVMVL+LICGRLVS W +++ V+ +E NFL RK++LYFVYG+RKPVQN
Sbjct: 322 GKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQN 381

Query: 443 CIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVLASSFH 502
           C+WLGLVLIAW  LF+K+VE E   TVLKYV +VL+ LLV+ +IWL+KTL+VKVLASSFH
Sbjct: 382 CLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFH 441

Query: 503 VSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIPPDLKAD 562
           +STYFDRIQESLF QYVIETLSGPP IEI      EE++A++V+  +  G  + P L   
Sbjct: 442 MSTYFDRIQESLFTQYVIETLSGPPRIEIHIE---EEKVANDVKTFEIVGRKLSP-LGPK 501

Query: 563 TSSVKSGRVIGGGVNQKSSH--GKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNVSAWNM 622
             S      +G G  QKS    GKS     S    + G +GI IDHL +++ KNVSAW M
Sbjct: 502 AVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKM 561

Query: 623 KRLLNMVRYGSISTLDEQIPGPCL-EDESTREIRSEREAKAAAKKIFQNVARRELKYIYP 682
           K+L+N+++ G++STLDEQI      ED+   +IRSE EAK AA+KIFQNVA    +YIY 
Sbjct: 562 KKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYM 621

Query: 683 EDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAVDR 742
           ED MRF+ EDE  R M LFEGA+E  +ISKS LKNWVVNAFRERR+LALTLNDTKTAV+R
Sbjct: 622 EDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNR 681

Query: 743 LHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLFVM 802
           LH +V+V+  I+ILI+WL++LGIA++KF   +SSQ+++V F+FGN+CKTIFEA+IF+FVM
Sbjct: 682 LHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVM 741

Query: 803 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 862
           HPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ PNS+L TK I N+YRSPDM ++
Sbjct: 742 HPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDA 801

Query: 863 VEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHRMN 922
           +EF +HIATP E    ++QRI+SY++  K HW PSPM VF+D+  LN +++A+W +H+MN
Sbjct: 802 IEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMN 861

Query: 923 HQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAP 949
           HQ+ GER+ RR  L+E++ ++C+ELDI+YRL P++INV SLP++ P
Sbjct: 862 HQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATP 865

BLAST of MC04g0366 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 780.4 bits (2014), Expect = 1.6e-225
Identity = 457/922 (49.57%), Postives = 593/922 (64.32%), Query Frame = 0

Query: 58  DPSDRTEVILKI---DDGGSAAVSRPVEGNGGKVWRETNYDFWNNDGKGENGASRASGAS 117
           D +DR + I+ I   + G   A        GG +W+E++YDFW+ + KG+N         
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGE-KGKN--------- 64

Query: 118 RVSGARASDNGDRNEGFEFVQRG----YGVEVEDPPMKLIGEFLHKQKLRG-ETTLDMDL 177
                +  D  +    F F QRG       E+ DPP KLIG+FLHKQ+  G E +LD++L
Sbjct: 65  ---DKKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVEL 124

Query: 178 EMEELQQDRIIPPLAESPLSQTSKDLKVSFQQDSTESS--SNDSIRRRYRDSRELQDEYK 237
            M ELQ +   PP   +  +   + L    +  S   +    D++RRR            
Sbjct: 125 NMAELQSN--TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR------------ 184

Query: 238 GQQSPWQQSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRL 297
                  Q+     GS    G   D  AE  +C S           +   L R KTKSRL
Sbjct: 185 -------QNRTSLGGSSDEEGRNRD-EAEVLKCGS-----------KKPMLSRNKTKSRL 244

Query: 298 MDPP-------EEPALLSGLIPKSGPLRSGFLGKG------------EEEDDDPFLEEDL 357
            DPP       ++  + SG   +SG  +SGFLGK             EEE++DPFL+EDL
Sbjct: 245 QDPPTPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDL 304

Query: 358 PEDFKRSDFSVLTLLQWISLVLITGALVCTLYIPYLRAMSLWELNIWKWEVMVLILICGR 417
           PE+FKR   S    L+WISLVLI  +LVC+L I  L+  + W+L++WKWEV VL+LICGR
Sbjct: 305 PEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGR 364

Query: 418 LVSGWGIRIIVFCIERNFLLRKRLLYFVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESET 477
           LVS W +RIIVF +E+NF  RKR+LYFVYGVRK VQNC+WLGLVL+AW  LF+K+VE ET
Sbjct: 365 LVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERET 424

Query: 478 NGTVLKYVRRVLVSLLVSTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 537
             T L+                                 TYFDRIQESLF QYVIETLSG
Sbjct: 425 RSTALR---------------------------------TYFDRIQESLFTQYVIETLSG 484

Query: 538 PPLIEIRKNEEAEERLADEVQKLQN-AGVSIPPDLKADTSSVKSGRVIGGGVNQKSSHGK 597
           PPL+EI++ EE E+++A++V+ L+  AG  +PP LKA   +VKS   +G       S G 
Sbjct: 485 PPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKA---TVKSFMKVG------KSPGL 544

Query: 598 SSHGKSSRPLTKNGND--GITIDHLHKLSPKNVSAWNMKRLLNMVRYGSISTLDEQIPGP 657
           +  G      +K G D  GI ID L +++ KNVSAWNMKRL+N++  G+ISTLD+ +   
Sbjct: 545 NRIG------SKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT 604

Query: 658 CLEDESTREIRSEREAKAAAKKIFQNVARRELKYIYPEDLMRFMREDEVLRTMSLFEGAT 717
             EDE    IRSE EAK AA+KIF NV     +YIY ED +RF+ E+E  R M+LFEGA+
Sbjct: 605 TQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGAS 664

Query: 718 ESRRISKSSLKNWVVNAFRERRSLALTLNDTKTAVDRLHHMVNVIFGILILILWLILLGI 777
           ES +ISKS LKNWV  AFRERR+LALTLNDTKTAVDRLH ++NV+ GI+I+I+WL++LGI
Sbjct: 665 ESDKISKSCLKNWV--AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGI 724

Query: 778 ASSKFFAFVSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNI 837
           A+++F   +SSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNI
Sbjct: 725 ATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNI 784

Query: 838 LTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIIS 897
           LTTVFLRYDN K+I PNSVL TK I N+YRSPDMG++VEFC+HIATP E I  +KQRI+S
Sbjct: 785 LTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILS 828

Query: 898 YIEGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQ 948
           Y++  K +W P+PM VF  +++LN +++AVWL+HRMNHQD GER+ RR +L+E+V K C+
Sbjct: 845 YVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCR 828

BLAST of MC04g0366 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 740.7 bits (1911), Expect = 1.4e-213
Identity = 406/773 (52.52%), Postives = 552/773 (71.41%), Query Frame = 0

Query: 195 LKVSFQQDSTESSSNDSIRRRYRDSRELQDEYKGQQSPWQQSHHERHGSPTISGVQNDCA 254
           L V+ + + TE  SN++       S+E +  +K   S          G+  +SG    C 
Sbjct: 83  LDVNEETEETEDVSNNN---NLSGSKETRVFFKINSS----------GTNNMSGSVRSCT 142

Query: 255 AEA--ARCTSNLSFQREL-----------SFQRTSHLLRAKTKSRLMDPP-EEPALLSGL 314
           +    +  T  L+ +++L           S ++T  + RAK +SRL+DPP EE    S  
Sbjct: 143 SSTSFSSATMRLNLEQQLEDEGEVVVRCSSVRKTELVSRAKARSRLIDPPQEEEQQYSSW 202

Query: 315 IPKSGPLRSGFLGKGE---EEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVC 374
           I  S  LRSG LG+     EE+DD   EED+P ++++     +TLLQW+SL+ +  ALV 
Sbjct: 203 IGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVL 262

Query: 375 TLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVY 434
           +L +   R  +LW L++WKWEV++L+LICGRLVSG GIRIIVF IERNFLLRKR+LYFVY
Sbjct: 263 SLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVY 322

Query: 435 GVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMV 494
           GV+  VQNC+WLGLVL+AW  LF+K+VE ET   VL  + ++LV  L+ST++WL+KTL+V
Sbjct: 323 GVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVV 382

Query: 495 KVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVS 554
           KVLASSFHVSTYFDRIQE+LF+ Y+IETLSGPP++E+ + EE E+R  DE+ K+Q  G  
Sbjct: 383 KVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGAD 442

Query: 555 IPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGND-GITIDHLHKLSPK 614
           + P+L                  Q+ S G + + K S  + K G+D GIT+D LHK++ K
Sbjct: 443 LSPEL------------CSAAFPQEKS-GSTMNMKFSPIIPKTGSDNGITMDDLHKMNQK 502

Query: 615 NVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRE 674
           NVSAWNMKRL+ +VR  S+STLDEQ      EDESTR+IRSE+EAKAAA+KIF+NVA+  
Sbjct: 503 NVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDESTRQIRSEKEAKAAARKIFKNVAQPG 562

Query: 675 LKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLNDT 734
            K+IY EDLMRF+R DE ++TM LFEGA  +++I+KS+LKNW+VNAFRERR+LALTLNDT
Sbjct: 563 TKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDT 622

Query: 735 KTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEAI 794
           KTAV++LHHM++ +  I+I+++WLILL IA+SK+  F++SQ+V++AF+FGN+ KT+FE+I
Sbjct: 623 KTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESI 682

Query: 795 IFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRS 854
           IFLF++HP+DVGDR  ID ++MVVEEMNILTTVFLR DNLK++ PN +L  K IHN+ RS
Sbjct: 683 IFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRS 742

Query: 855 PDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAVW 914
           PDMG+ V  C+HI TP E IA +KQRI SYI+    +W P    + KDVE+LN +R+A+W
Sbjct: 743 PDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIW 802

Query: 915 LSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPS 950
           L H++NHQ+ GER+ RR++L+E+V+K+  ELDIQYR  P+DINV ++P+   S
Sbjct: 803 LCHKINHQNMGERFTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSS 829