MC04g0324 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0324
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein kinase domain-containing protein
LocationMC04: 2569031 .. 2572664 (+)
RNA-Seq ExpressionMC04g0324
SyntenyMC04g0324
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCAATGGCGGAAAATGGCGACGCCATAATTTCTCGACCCCACCACAGAAGAAGTAACATTCATATATCTTTATTTCTCAATTCCTTTTCATTATCTTTGAATTCAATTTTGCACGTAGTCAGATTGAATCCAAACAACCATCATGCACGGCAGTTGACTTTGAACAACCAGACAGATTTCCAGTCTCTTGTCATCTTCTACAGCCCCCACCACCTTTCAATTTCAGCGACTCGGTCTTCCTGGTAAATCGCCTCTATTTTTCCCCTCTTCTGCTCTTTTCACCGCAGTTTATGCAATCGCTTAGGTATTTGCCCTCGATTTTGTGAAACTGTTTGGGGGATTTCTGAATTGATTGGTCCAGGCTTATACGGGACGGGAAAAGATGAAGTAGTTGACTGAGAAGTTTGGATTGCTTTTTATTTTACTTCATAAGGGCTCTCCTTATCTTCTTCTTCTTCTTCTTCCTTCCTCTTGTATCCAACAGGAAGAGAAAGAGAGAGCGATAATTCATGGGGATTTGTTGGGGTTCTCAAGTTCAAAATCATACCCCAAATACCACTGGAAATCTTAGTTCAGGTAATTATTTTGTTACTCTAATTTTCTGTGTTCTTAAGTTTGTGCTATTTATGCCCAAATAATTTTCCCCTCCTGACTTTGAAGTTCTTTGATGGGATCTTTTTTAGTACTGTTTATCAATCTTTGAGCAGGAGATATTTTCTGGGACTGTTTACCAATCTGTTCCTTTGTGTTTGTTTAATCTGCTTGGGAGTTTTCTAATTTTTGCGTGTTTGGTTTGTTTTTCAATTGGATTTTCATCCAAAAGCTGATTGCAAATAGTGATATCTTTGTTTATAACTTGGAAAAATTTACGACTCAAATTTGTGGCCATAAACAGTGGTATCTCAGACAACTAGTCAGACAACATCCTCAACAGTCAGCAACATCTCGAGGAACAGTCTGTTCTCAGCAGCGAGTGGAGACGAAGCTTATCCAAATGGCCAGATTTTGCCCTCTCCGAACTTGAAGGAGTATAGCTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGACCTGAGGCATTGCTTGGCGAGGGAGGTTTTGGGAAGGTTTACAAAGGTTGGCTTGAGGAGAAGGGCTATGGAAAAAGGGGGAATCCGATGGTTATTGCGGTCAAAAAGTTGAAATCTGATAGCATGCAAGGAATTGAGGAGTGGCAGGTATTATATTGTTATAGAGAAGAAAACTCTCTTCTCTTCTCTGAGATTTTCTTGAACAAAAGACCGAGAAAAATATCTGTTCAAAAATTGTTCAGTCTGCGTCCTAGTTCAAGTTCTTAGGCCGAAAACTTAATAATTTGCCTTAACGTGAGCAAAGTCTCCCTAAGAATTATTGGAGTGAACCGAGAGCATAATACAAGAAGTGAGCACTGATACCATGGTTATCAAAAAGAAAAAACCGAAAGAAGCAATTATTATTCACTCCAGAGAGAATCACTTTGGGTAGAGGATTACGTGAATCAATTGTGAATTACTTTAGTTTGAGTGTATTTTTGGCGGAACAGTTCTTTAGACCTGCACTTGAAAATGGTTCCTTCATTGTTCACCAGTTACTGAACACTTCTTCCCTCAGTTCATGGTTCTGTTTTTGCTTGTTCTTAAGGTTTTTTTTTCCTTTCAAGTTTGAAGACGATTTTGAGAACAACTCAACTGCAAACGACAAATTTTTAATAAAAAAGCACCATTCTTAATGGATTTTTTGTATTAAAAAACTGAGGACAGTTTGTTTACATCCTTCCACAAAAAAAAAAAAAAAAGAACACGACCTTAATGTGATTTCCATCATTCATGTTTCTGTCATTGAAATACTGCTAACCTTTGGCAGTGGCAACTAGCATAGATCAGTTAAGAAGTAGGCAGATTAAGCTTCCATAATTGCTGACTTTAGAGTGAATCGCTTTGAGTATGAGACTATATAACTTCCTCACTGTGTAACTTCTATGATTTTCTGTTTTCCTTGTTTGTATTAATATATACAATATCTACCTCTGGCAACTCCCATAAATCAGATCAAATATTTAAACTAATTCTCAAGACTTTTTGAAAAATATACATATAATAACCTTGTCTGGTTGGTTGTGCAGTCAGAAGTGGGCTTCTTGGGAAGACTCTCTCACCCAAATCTAGTGAAGCTGTTGGGTTACTGTTGGGAGGATCATCAGTTCCTCCTCGCCTATGAGTTCATGCAAAAAGGAAGCTTAGAGAACCATCTATTTGGAAGTAAGAAAACTAATTGGATGATATTATACTATACTATGATGTTTCTTTCACTTAATTGCTAGTTTCTTCTCTTTGTTCTCACCAACAGAGAGATGATTCATAGTTTACTGTAAACTGTAAATAACCTTACTGTGAAATGCAGGGGGATCGGCAGTTACACCGCTACAGTGGGATACTCGGCTTAAGGTCGCCATAGGTGCTGCCCGAGGTCTGGCCTTCTTGCACACATCAGACGAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTTGACGGGGTAAAGTTATATTTACTCATCTCAATCTTTCTTTGACAACCTTTCTCATATCACTGTTTCTTTTCTGCGAAAGGAAGGCTTTCGGTATCTTCTCTCCTTATAGTATTTGTCGTAATCCGAATCTACCTTTAACTGCAGTCATATACAGCAAAGCTGTCTGACTTCGGCTTGGCTAAATTGGGTCCATCAGAAAGTAAATCTCATTTGACAACTCGTGTTATGGGCACTCATGGTTATGCTGCCCCGGAGTATGTTACCACAGGTAGAATAAAAACCTTTCAAAATTAACTGTGTTAAGAAACTGGAGTCTGTTATCACTGAGAGATTTGTGAGCTGATGTTTATGACCGCGTTTATAATACAGGACATCTGTACGTGAAGAGTGATGTCTATGGTTTTGGAGTCGTACTGATCGAGATGCTGACCGGTTTACGTGCGCTTGATGAAAATCGTCCAACCGGGCAAGAAAACCTGCTGGATTGGATAAAACCATACTTATCAGAGAGGAGGAGGCTGAAAAATGTGATGGACTTTCGCTTGGAAGGAAAATATCCATCCAGATCTGCATTCCAAGTGGCTCAGCTAGCTCTCCAATGCATCGAACACGAACAGAGAAACCGACCATCAATGAAAGAGGTCGTTGAGACATTGGAACAGATCGAAGCCACTAACGAGAAACCAACCGAGCCTAGAAACCGTGCGACCCGTGTGGCGTCAAATCGAAATGCTCATCAGCCTCTTCACCACAACTCTCCACTTCACATGAAGAAAAATGGAATCCATGCTAGTGAAACACCACCCAGGTTGAGATAAATTTGGGTGGTTCTGATTCTCTTATCACACCCTTATTTCTTCATTTTTATGGTTCTCATAGATGTTAGAATGAAGTTTGTTAACTTTTTGGGTAATATGTAATTAAATACTACTTAGGCTGTGGATGATGAGAATTAGGACAGGTTCTGATTGTTACAATTTACACTGGATTGTCAAGTTTGATTGATCTTTGAAGGCAATCTGTTCTTATTTTTCTGAAGTTAGTAAAAACTTGAATGGTATCTCTAAAAGAAAGG

mRNA sequence

CCTCCAATGGCGGAAAATGGCGACGCCATAATTTCTCGACCCCACCACAGAAGAAATTGAATCCAAACAACCATCATGCACGGCAGTTGACTTTGAACAACCAGACAGATTTCCAGTCTCTTGTCATCTTCTACAGCCCCCACCACCTTTCAATTTCAGCGACTCGGTCTTCCTGGTAAATCGCCTCTATTTTTCCCCTCTTCTGCTCTTTTCACCGCAGTTTATGCAATCGCTTAGGTATTTGCCCTCGATTTTGTGAAACTGTTTGGGGGATTTCTGAATTGATTGGTCCAGGCTTATACGGGACGGGAAAAGATGAAGTAGTTGACTGAGAAGTTTGGATTGCTTTTTATTTTACTTCATAAGGGCTCTCCTTATCTTCTTCTTCTTCTTCTTCCTTCCTCTTGTATCCAACAGGAAGAGAAAGAGAGAGCGATAATTCATGGGGATTTGTTGGGGTTCTCAAGTTCAAAATCATACCCCAAATACCACTGGAAATCTTAGTTCAGTGGTATCTCAGACAACTAGTCAGACAACATCCTCAACAGTCAGCAACATCTCGAGGAACAGTCTGTTCTCAGCAGCGAGTGGAGACGAAGCTTATCCAAATGGCCAGATTTTGCCCTCTCCGAACTTGAAGGAGTATAGCTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGACCTGAGGCATTGCTTGGCGAGGGAGGTTTTGGGAAGGTTTACAAAGGTTGGCTTGAGGAGAAGGGCTATGGAAAAAGGGGGAATCCGATGGTTATTGCGGTCAAAAAGTTGAAATCTGATAGCATGCAAGGAATTGAGGAGTGGCAGTCAGAAGTGGGCTTCTTGGGAAGACTCTCTCACCCAAATCTAGTGAAGCTGTTGGGTTACTGTTGGGAGGATCATCAGTTCCTCCTCGCCTATGAGTTCATGCAAAAAGGAAGCTTAGAGAACCATCTATTTGGAAGGGGATCGGCAGTTACACCGCTACAGTGGGATACTCGGCTTAAGGTCGCCATAGGTGCTGCCCGAGGTCTGGCCTTCTTGCACACATCAGACGAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTTGACGGGTCATATACAGCAAAGCTGTCTGACTTCGGCTTGGCTAAATTGGGTCCATCAGAAAGTAAATCTCATTTGACAACTCGTGTTATGGGCACTCATGGTTATGCTGCCCCGGAGTATGTTACCACAGGACATCTGTACGTGAAGAGTGATGTCTATGGTTTTGGAGTCGTACTGATCGAGATGCTGACCGGTTTACGTGCGCTTGATGAAAATCGTCCAACCGGGCAAGAAAACCTGCTGGATTGGATAAAACCATACTTATCAGAGAGGAGGAGGCTGAAAAATGTGATGGACTTTCGCTTGGAAGGAAAATATCCATCCAGATCTGCATTCCAAGTGGCTCAGCTAGCTCTCCAATGCATCGAACACGAACAGAGAAACCGACCATCAATGAAAGAGGTCGTTGAGACATTGGAACAGATCGAAGCCACTAACGAGAAACCAACCGAGCCTAGAAACCGTGCGACCCGTGTGGCGTCAAATCGAAATGCTCATCAGCCTCTTCACCACAACTCTCCACTTCACATGAAGAAAAATGGAATCCATGCTAGTGAAACACCACCCAGGTTGAGATAAATTTGGGTGGTTCTGATTCTCTTATCACACCCTTATTTCTTCATTTTTATGGTTCTCATAGATGTTAGAATGAAGTTTGTTAACTTTTTGGGTAATATGTAATTAAATACTACTTAGGCTGTGGATGATGAGAATTAGGACAGGTTCTGATTGTTACAATTTACACTGGATTGTCAAGTTTGATTGATCTTTGAAGGCAATCTGTTCTTATTTTTCTGAAGTTAGTAAAAACTTGAATGGTATCTCTAAAAGAAAGG

Coding sequence (CDS)

ATGGGGATTTGTTGGGGTTCTCAAGTTCAAAATCATACCCCAAATACCACTGGAAATCTTAGTTCAGTGGTATCTCAGACAACTAGTCAGACAACATCCTCAACAGTCAGCAACATCTCGAGGAACAGTCTGTTCTCAGCAGCGAGTGGAGACGAAGCTTATCCAAATGGCCAGATTTTGCCCTCTCCGAACTTGAAGGAGTATAGCTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGACCTGAGGCATTGCTTGGCGAGGGAGGTTTTGGGAAGGTTTACAAAGGTTGGCTTGAGGAGAAGGGCTATGGAAAAAGGGGGAATCCGATGGTTATTGCGGTCAAAAAGTTGAAATCTGATAGCATGCAAGGAATTGAGGAGTGGCAGTCAGAAGTGGGCTTCTTGGGAAGACTCTCTCACCCAAATCTAGTGAAGCTGTTGGGTTACTGTTGGGAGGATCATCAGTTCCTCCTCGCCTATGAGTTCATGCAAAAAGGAAGCTTAGAGAACCATCTATTTGGAAGGGGATCGGCAGTTACACCGCTACAGTGGGATACTCGGCTTAAGGTCGCCATAGGTGCTGCCCGAGGTCTGGCCTTCTTGCACACATCAGACGAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTTGACGGGTCATATACAGCAAAGCTGTCTGACTTCGGCTTGGCTAAATTGGGTCCATCAGAAAGTAAATCTCATTTGACAACTCGTGTTATGGGCACTCATGGTTATGCTGCCCCGGAGTATGTTACCACAGGACATCTGTACGTGAAGAGTGATGTCTATGGTTTTGGAGTCGTACTGATCGAGATGCTGACCGGTTTACGTGCGCTTGATGAAAATCGTCCAACCGGGCAAGAAAACCTGCTGGATTGGATAAAACCATACTTATCAGAGAGGAGGAGGCTGAAAAATGTGATGGACTTTCGCTTGGAAGGAAAATATCCATCCAGATCTGCATTCCAAGTGGCTCAGCTAGCTCTCCAATGCATCGAACACGAACAGAGAAACCGACCATCAATGAAAGAGGTCGTTGAGACATTGGAACAGATCGAAGCCACTAACGAGAAACCAACCGAGCCTAGAAACCGTGCGACCCGTGTGGCGTCAAATCGAAATGCTCATCAGCCTCTTCACCACAACTCTCCACTTCACATGAAGAAAAATGGAATCCATGCTAGTGAAACACCACCCAGGTTGAGATAA

Protein sequence

MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQILPSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKSDSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAVTPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQENLLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETLEQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR
Homology
BLAST of MC04g0324 vs. ExPASy Swiss-Prot
Match: Q9SII6 (Probable serine/threonine-protein kinase PIX13 OS=Arabidopsis thaliana OX=3702 GN=PIX13 PE=1 SV=2)

HSP 1 Score: 535.8 bits (1379), Expect = 4.3e-151
Identity = 277/409 (67.73%), Postives = 327/409 (79.95%), Query Frame = 0

Query: 1   MGICWGSQVQN---HTPNTTGNLSSV----VSQTTSQTTSSTVSNISRNSLFSAASGDEA 60
           MG+CWGS   +    TP++TGN+SSV     S  T+ T +S  SNIS NS FS ASG++A
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSVGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDA 60

Query: 61  YPNGQILPSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVI 120
           YP+GQILP PNL+ +SLAEL+A+T+NFR E +LGEGGFGKV+KGWLE+K  GK+ N  VI
Sbjct: 61  YPDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVI 120

Query: 121 AVKKLKSDSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHL 180
           AVKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E  + LL YE+MQKGSLENHL
Sbjct: 121 AVKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHL 180

Query: 181 FGRGSAVTPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFG 240
           F +GSAV PL W+ RLK+AIGAA+GLAFLH S++QVIYRDFKASNILLDGSY AK+SDFG
Sbjct: 181 FRKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFG 240

Query: 241 LAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDEN 300
           LAKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  
Sbjct: 241 LAKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPT 300

Query: 301 RPTGQENLLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSM 360
           RPTGQ NL +WIKP+LSERR+L+++MD RLEGKYP +SAF+VAQLAL+C+  E +NRPSM
Sbjct: 301 RPTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSM 360

Query: 361 KEVVETLEQIEATNEKPTEPR-NRATRVASNRNAHQPLHHNSPLHMKKN 402
           KEVVE+LE IEA NEKP E R  RA+     +  H      S    ++N
Sbjct: 361 KEVVESLELIEAANEKPLERRTTRASPSIRQQQGHYRPQQLSSFRPRQN 409

BLAST of MC04g0324 vs. ExPASy Swiss-Prot
Match: P27450 (Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=CST PE=1 SV=2)

HSP 1 Score: 408.7 bits (1049), Expect = 7.9e-113
Identity = 207/383 (54.05%), Postives = 281/383 (73.37%), Query Frame = 0

Query: 12  HTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQILPSPNLKEYSLA 71
           H+  TT N S+  ++ +S T ++T S++ + S FS  S      +G++L SPNLK Y+  
Sbjct: 19  HSHATTNNHSN-GTEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNFL 78

Query: 72  ELKAATKNFRPEALLGEGGFGKVYKGWLEEK--GYGKRGNPMVIAVKKLKSDSMQGIEEW 131
           +LK ATKNF+P+++LG+GGFGKVY+GW++       + G+ M++A+K+L S+S+QG  EW
Sbjct: 79  DLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAEW 138

Query: 132 QSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAVTPLQWDTRL 191
           +SEV FLG LSH NLVKLLGYC ED + LL YEFM KGSLE+HLF R     P  WD R+
Sbjct: 139 RSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRI 198

Query: 192 KVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTR 251
           K+ IGAARGLAFLH+   +VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TTR
Sbjct: 199 KIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTR 258

Query: 252 VMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQENLLDWIKPYL 311
           +MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE+L+DW++P L
Sbjct: 259 IMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPEL 318

Query: 312 SERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETLEQIEATNEK 371
           S + R+K +MD  ++G+Y ++ A ++A++ L CIE + +NRP MKEVVE LE I+  N  
Sbjct: 319 SNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLNVV 378

Query: 372 PTEPRNRATRVASNRNAHQPLHH 393
           P   R+   +  +N +   P H+
Sbjct: 379 PN--RSSTKQAVANSSRSSPHHY 395

BLAST of MC04g0324 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.1e-109
Identity = 201/388 (51.80%), Postives = 273/388 (70.36%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MG CWG+++ + +P           ++ S  +  T    S +S+ +A+         +IL
Sbjct: 1   MGNCWGAKISSESP----------CRSASSPSGGTSKYASNSSVSAASVPPTPRSEDEIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYG--KRGNPMVIAVKKL 120
            + N+K ++  EL+ AT+NFRP+++LGEGGFG V+KGW++EK     K G  MVIAVKKL
Sbjct: 61  EAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIAVKKL 120

Query: 121 KSDSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGS 180
             +  QG  EW +EV +LG+LSHP LV+L+GYC ED Q LL YEFM +GSLENHLF R +
Sbjct: 121 NQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLFRRST 180

Query: 181 AVTPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLG 240
              PL W+ R+K+A+GAA+GLAFLH+   +VIYRDFK SN+LLD +Y AKLSDFGLAK G
Sbjct: 181 HFQPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGLAKDG 240

Query: 241 PSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQ 300
           P+  KSH++TRVMGT+GYAAPEY+ TGHL  KSDVY FGVVL+EML+G RALD+NRPTG+
Sbjct: 241 PTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNRPTGE 300

Query: 301 ENLLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVE 360
            NL++W +PYL  +RR+  ++D RL G+Y    A + A LALQCI  E +NRP+M++VV 
Sbjct: 301 HNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLALQCISVEAKNRPNMEQVVA 360

Query: 361 TLEQIEATNEKPTEPRNRATRVASNRNA 387
            LEQ++ + E    P  +  + +S++NA
Sbjct: 361 VLEQLQDSKETGANP--QLQKKSSSKNA 376

BLAST of MC04g0324 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 9.1e-109
Identity = 203/370 (54.86%), Postives = 263/370 (71.08%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGIC  +Q++  +P  +    S  +  +  + SS+VS I  N              G+IL
Sbjct: 1   MGICLSAQIKAVSPGASPKYMSSEANDSLGSKSSSVS-IRTN----------PRTEGEIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGY--GKRGNPMVIAVKKL 120
            SPNLK ++ AELKAAT+NFRP+++LGEGGFG V+KGW++E+     K G  +VIAVKKL
Sbjct: 61  QSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKL 120

Query: 121 KSDSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGS 180
             D  QG +EW +EV +LG+ SHPNLVKL+GYC ED   LL YEFM +GSLENHLF RGS
Sbjct: 121 NQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGS 180

Query: 181 AVTPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLG 240
              PL W  RLKVA+GAA+GLAFLH ++  VIYRDFK SNILLD  Y AKLSDFGLAK G
Sbjct: 181 YFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDG 240

Query: 241 PSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQ 300
           P+  KSH++TR+MGT+GYAAPEY+ TGHL  KSDVY +GVVL+E+L+G RA+D+NRP G+
Sbjct: 241 PTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGE 300

Query: 301 ENLLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVE 360
           + L++W +P L+ +R+L  V+D RL+ +Y    A +VA LAL+C+  E + RP+M EVV 
Sbjct: 301 QKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVS 359

Query: 361 TLEQIEATNE 369
            LE I+  NE
Sbjct: 361 HLEHIQTLNE 359

BLAST of MC04g0324 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 394.0 bits (1011), Expect = 2.0e-108
Identity = 201/377 (53.32%), Postives = 271/377 (71.88%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MG C+ ++++    ++T   S  +S+  S+  SST S       FS     E    G+IL
Sbjct: 1   MGGCFSNRIKTDIASSTWLSSKFLSRDGSK-GSSTAS-------FSYMPRTE----GEIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGY--GKRGNPMVIAVKKL 120
            + NLK +SL+ELK+AT+NFRP++++GEGGFG V+KGW++E      K G  +VIAVK+L
Sbjct: 61  QNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRL 120

Query: 121 KSDSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGS 180
             +  QG  EW +E+ +LG+L HPNLVKL+GYC E+   LL YEFM +GSLENHLF RG+
Sbjct: 121 NQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGT 180

Query: 181 AVTPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLG 240
              PL W+TR+++A+GAARGLAFLH +  QVIYRDFKASNILLD +Y AKLSDFGLA+ G
Sbjct: 181 FYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDG 240

Query: 241 PSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQ 300
           P    SH++TRVMGT GYAAPEY+ TGHL VKSDVY FGVVL+E+L+G RA+D+N+P G+
Sbjct: 241 PMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGE 300

Query: 301 ENLLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVE 360
            NL+DW +PYL+ +RRL  VMD RL+G+Y    A ++A LAL CI  + ++RP+M E+V+
Sbjct: 301 HNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVK 360

Query: 361 TLEQIEATNEKPTEPRN 376
           T+E++    E   E +N
Sbjct: 361 TMEELHIQKEASKEQQN 365

BLAST of MC04g0324 vs. NCBI nr
Match: XP_022136188.1 (probable serine/threonine-protein kinase PIX13 [Momordica charantia])

HSP 1 Score: 818 bits (2112), Expect = 1.09e-298
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL
Sbjct: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS
Sbjct: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL
Sbjct: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360

Query: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR
Sbjct: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413

BLAST of MC04g0324 vs. NCBI nr
Match: XP_038886859.1 (probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886860.1 probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886861.1 probable serine/threonine-protein kinase PIX13 [Benincasa hispida])

HSP 1 Score: 749 bits (1935), Expect = 1.03e-271
Identity = 373/413 (90.31%), Postives = 397/413 (96.13%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTPNTTG+LSSVVSQTT+QTTSST+SNIS+NS FSAASGDE +P+GQIL
Sbjct: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTNQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PS NL+EYSLAELKAATKNFR EA+LGEGGFGKVYKGWLEEKG G++G  MVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGLGRKGYSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL+WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L+DWIKPYLSERR+LKNVMDFRLEGKYPSRSA+QVAQLALQCIE EQ+NRPSMKEVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAYQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIE  NEKP EPRNRA+R+ASNRNAHQPLHHNSPLHMK+ GIHA++TPPRLR
Sbjct: 361 EQIETANEKPIEPRNRASRLASNRNAHQPLHHNSPLHMKQYGIHANQTPPRLR 413

BLAST of MC04g0324 vs. NCBI nr
Match: XP_008447841.1 (PREDICTED: probable serine/threonine-protein kinase Cx32, chloroplastic [Cucumis melo] >TYK23285.1 putative serine/threonine-protein kinase Cx32 [Cucumis melo var. makuwa])

HSP 1 Score: 734 bits (1894), Expect = 1.89e-265
Identity = 370/414 (89.37%), Postives = 393/414 (94.93%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTP+TTG+LSSV+SQTTSQTTSST+SNIS+NS FSAASGDE +P+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PS NL+EYSLAELKAATKNFR EALLGEGGFGKVYKGWLEEKG G++GN MVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L+DWIKP+LSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRPSMKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEATNEKPTEP-RNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIE  NEK  E  RNRA+R+ASNRNAHQPLHHNSPLHMK+ G HA++TPPR R
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of MC04g0324 vs. NCBI nr
Match: XP_004154222.1 (probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_011658631.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_031744213.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_031744214.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >KAE8645697.1 hypothetical protein Csa_020515 [Cucumis sativus])

HSP 1 Score: 726 bits (1874), Expect = 2.03e-262
Identity = 364/413 (88.14%), Postives = 389/413 (94.19%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTP+TTG+ SSV+SQTTS TTSST+SNIS+NS FSAASGDE +P+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PS NL+EYSLAELKAATKNFR EALLGEGGFGKVYKGWLEEKG G++GN MVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QG+EEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGLEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L +WIKP+LSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRPSMKEVVETL
Sbjct: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIE  NEK  E  NRA+R+ASNRNAHQPLHHNSPLHMK+ G HA++TPPR R
Sbjct: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 413

BLAST of MC04g0324 vs. NCBI nr
Match: KAG6589052.1 (putative serine/threonine-protein kinase PIX13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022765.1 putative serine/threonine-protein kinase PIX13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 706 bits (1822), Expect = 1.08e-254
Identity = 355/413 (85.96%), Postives = 384/413 (92.98%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTPNTTG+LSSVVSQTTS    STVSNIS+NS FS ASGDEA+PNGQIL
Sbjct: 1   MGICWGSPVGNHTPNTTGHLSSVVSQTTS----STVSNISKNSNFSVASGDEAFPNGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PSPNL+EYSLAELKAATKNFR EA+LGEGGFGKVYKGWLEEKG+GK+GN MVIAVKKLKS
Sbjct: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGHGKKGNSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL+WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYT KLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTPKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHL+TRVMGT+GYAAPEY++TGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG EN
Sbjct: 241 ESKSHLSTRVMGTNGYAAPEYISTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGHEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L+DWIKPYLSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRP+M EVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPAMNEVVETL 360

Query: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIEA N++P EPRNR+ R        QPLHH+SPLH+K+ G+HA++T PRLR
Sbjct: 361 EQIEAMNDRPNEPRNRSPR--------QPLHHSSPLHVKQYGVHANQTSPRLR 401

BLAST of MC04g0324 vs. ExPASy TrEMBL
Match: A0A6J1C6Y9 (probable serine/threonine-protein kinase PIX13 OS=Momordica charantia OX=3673 GN=LOC111007944 PE=3 SV=1)

HSP 1 Score: 818 bits (2112), Expect = 5.30e-299
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL
Sbjct: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS
Sbjct: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL
Sbjct: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360

Query: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR
Sbjct: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413

BLAST of MC04g0324 vs. ExPASy TrEMBL
Match: A0A5D3DI79 (Putative serine/threonine-protein kinase Cx32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G003430 PE=3 SV=1)

HSP 1 Score: 734 bits (1894), Expect = 9.16e-266
Identity = 370/414 (89.37%), Postives = 393/414 (94.93%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTP+TTG+LSSV+SQTTSQTTSST+SNIS+NS FSAASGDE +P+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PS NL+EYSLAELKAATKNFR EALLGEGGFGKVYKGWLEEKG G++GN MVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L+DWIKP+LSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRPSMKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEATNEKPTEP-RNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIE  NEK  E  RNRA+R+ASNRNAHQPLHHNSPLHMK+ G HA++TPPR R
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of MC04g0324 vs. ExPASy TrEMBL
Match: A0A1S3BHT1 (probable serine/threonine-protein kinase Cx32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103490212 PE=3 SV=1)

HSP 1 Score: 734 bits (1894), Expect = 9.16e-266
Identity = 370/414 (89.37%), Postives = 393/414 (94.93%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTP+TTG+LSSV+SQTTSQTTSST+SNIS+NS FSAASGDE +P+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PS NL+EYSLAELKAATKNFR EALLGEGGFGKVYKGWLEEKG G++GN MVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L+DWIKP+LSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRPSMKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEATNEKPTEP-RNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIE  NEK  E  RNRA+R+ASNRNAHQPLHHNSPLHMK+ G HA++TPPR R
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of MC04g0324 vs. ExPASy TrEMBL
Match: A0A6J1EKG2 (probable serine/threonine-protein kinase PIX13 OS=Cucurbita moschata OX=3662 GN=LOC111435287 PE=3 SV=1)

HSP 1 Score: 704 bits (1818), Expect = 2.13e-254
Identity = 354/413 (85.71%), Postives = 384/413 (92.98%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTPNTTG+LSSVVSQTTS    STVSNIS+NS FS ASGDEA+PNGQIL
Sbjct: 1   MGICWGSPVGNHTPNTTGHLSSVVSQTTS----STVSNISKNSNFSVASGDEAFPNGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PSPNL+EYSLAELKAATKNFR EA+LGEGGFGKVYKGWLEEKG+GK+GN MVIAVKKLKS
Sbjct: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGHGKKGNSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL+WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYT KLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTPKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHL+TRVMGT+GYAAPEY++TGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG EN
Sbjct: 241 ESKSHLSTRVMGTNGYAAPEYISTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGHEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L+DWIKPYLSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRP+M EVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPAMNEVVETL 360

Query: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           E+IEA N++P EPRNR+ R        QPLHH+SPLH+K+ G+HA++T PRLR
Sbjct: 361 ERIEAMNDRPNEPRNRSPR--------QPLHHSSPLHVKQYGVHANQTSPRLR 401

BLAST of MC04g0324 vs. ExPASy TrEMBL
Match: A0A6J1JQ74 (probable serine/threonine-protein kinase PIX13 OS=Cucurbita maxima OX=3661 GN=LOC111486471 PE=3 SV=1)

HSP 1 Score: 702 bits (1811), Expect = 2.48e-253
Identity = 355/413 (85.96%), Postives = 382/413 (92.49%), Query Frame = 0

Query: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60
           MGICWGS V NHTPNTTG+LSSVVSQTTS    STVSNIS+NS FS ASGDEA+PNGQIL
Sbjct: 1   MGICWGSPVGNHTPNTTGHLSSVVSQTTS----STVSNISKNSNFSVASGDEAFPNGQIL 60

Query: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120
           PSPNL+EYSLAELKAATKNFR EA+LGEGGFGKVYKGWLEEKG+GK+GN MVIAVKKLKS
Sbjct: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGHGKKGNSMVIAVKKLKS 120

Query: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL+WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYT KLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTPKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300
           ESKSHL+TRVMGT+GYAAPEY+TTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG EN
Sbjct: 241 ESKSHLSTRVMGTNGYAAPEYITTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGHEN 300

Query: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360
           L+DWIKPYLSERR+LKNVMDFRLEGKYPSRSAFQVA LALQCIE EQ+NRP+M EVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAHLALQCIEQEQKNRPAMNEVVETL 360

Query: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413
           EQIEA N++ TEPRNR+ R        QPLHH+SPLH+K+ G HA++T PRLR
Sbjct: 361 EQIEAMNDRLTEPRNRSPR--------QPLHHSSPLHVKQYGSHANQTSPRLR 401

BLAST of MC04g0324 vs. TAIR 10
Match: AT2G17220.1 (Protein kinase superfamily protein )

HSP 1 Score: 535.8 bits (1379), Expect = 3.1e-152
Identity = 277/409 (67.73%), Postives = 327/409 (79.95%), Query Frame = 0

Query: 1   MGICWGSQVQN---HTPNTTGNLSSV----VSQTTSQTTSSTVSNISRNSLFSAASGDEA 60
           MG+CWGS   +    TP++TGN+SSV     S  T+ T +S  SNIS NS FS ASG++A
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSVGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDA 60

Query: 61  YPNGQILPSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVI 120
           YP+GQILP PNL+ +SLAEL+A+T+NFR E +LGEGGFGKV+KGWLE+K  GK+ N  VI
Sbjct: 61  YPDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVI 120

Query: 121 AVKKLKSDSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHL 180
           AVKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E  + LL YE+MQKGSLENHL
Sbjct: 121 AVKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHL 180

Query: 181 FGRGSAVTPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFG 240
           F +GSAV PL W+ RLK+AIGAA+GLAFLH S++QVIYRDFKASNILLDGSY AK+SDFG
Sbjct: 181 FRKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFG 240

Query: 241 LAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDEN 300
           LAKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  
Sbjct: 241 LAKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPT 300

Query: 301 RPTGQENLLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSM 360
           RPTGQ NL +WIKP+LSERR+L+++MD RLEGKYP +SAF+VAQLAL+C+  E +NRPSM
Sbjct: 301 RPTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSM 360

Query: 361 KEVVETLEQIEATNEKPTEPR-NRATRVASNRNAHQPLHHNSPLHMKKN 402
           KEVVE+LE IEA NEKP E R  RA+     +  H      S    ++N
Sbjct: 361 KEVVESLELIEAANEKPLERRTTRASPSIRQQQGHYRPQQLSSFRPRQN 409

BLAST of MC04g0324 vs. TAIR 10
Match: AT2G17220.2 (Protein kinase superfamily protein )

HSP 1 Score: 534.3 bits (1375), Expect = 8.9e-152
Identity = 276/408 (67.65%), Postives = 326/408 (79.90%), Query Frame = 0

Query: 1   MGICWGSQVQN---HTPNTTGNLSSVV---SQTTSQTTSSTVSNISRNSLFSAASGDEAY 60
           MG+CWGS   +    TP++TGN+SS     S  T+ T +S  SNIS NS FS ASG++AY
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDAY 60

Query: 61  PNGQILPSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIA 120
           P+GQILP PNL+ +SLAEL+A+T+NFR E +LGEGGFGKV+KGWLE+K  GK+ N  VIA
Sbjct: 61  PDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVIA 120

Query: 121 VKKLKSDSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLF 180
           VKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E  + LL YE+MQKGSLENHLF
Sbjct: 121 VKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHLF 180

Query: 181 GRGSAVTPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGL 240
            +GSAV PL W+ RLK+AIGAA+GLAFLH S++QVIYRDFKASNILLDGSY AK+SDFGL
Sbjct: 181 RKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFGL 240

Query: 241 AKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENR 300
           AKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  R
Sbjct: 241 AKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPTR 300

Query: 301 PTGQENLLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMK 360
           PTGQ NL +WIKP+LSERR+L+++MD RLEGKYP +SAF+VAQLAL+C+  E +NRPSMK
Sbjct: 301 PTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSMK 360

Query: 361 EVVETLEQIEATNEKPTEPR-NRATRVASNRNAHQPLHHNSPLHMKKN 402
           EVVE+LE IEA NEKP E R  RA+     +  H      S    ++N
Sbjct: 361 EVVESLELIEAANEKPLERRTTRASPSIRQQQGHYRPQQLSSFRPRQN 408

BLAST of MC04g0324 vs. TAIR 10
Match: AT4G35600.1 (Protein kinase superfamily protein )

HSP 1 Score: 408.7 bits (1049), Expect = 5.6e-114
Identity = 207/383 (54.05%), Postives = 281/383 (73.37%), Query Frame = 0

Query: 12  HTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQILPSPNLKEYSLA 71
           H+  TT N S+  ++ +S T ++T S++ + S FS  S      +G++L SPNLK Y+  
Sbjct: 19  HSHATTNNHSN-GTEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNFL 78

Query: 72  ELKAATKNFRPEALLGEGGFGKVYKGWLEEK--GYGKRGNPMVIAVKKLKSDSMQGIEEW 131
           +LK ATKNF+P+++LG+GGFGKVY+GW++       + G+ M++A+K+L S+S+QG  EW
Sbjct: 79  DLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAEW 138

Query: 132 QSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAVTPLQWDTRL 191
           +SEV FLG LSH NLVKLLGYC ED + LL YEFM KGSLE+HLF R     P  WD R+
Sbjct: 139 RSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRI 198

Query: 192 KVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTR 251
           K+ IGAARGLAFLH+   +VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TTR
Sbjct: 199 KIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTR 258

Query: 252 VMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQENLLDWIKPYL 311
           +MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE+L+DW++P L
Sbjct: 259 IMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPEL 318

Query: 312 SERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETLEQIEATNEK 371
           S + R+K +MD  ++G+Y ++ A ++A++ L CIE + +NRP MKEVVE LE I+  N  
Sbjct: 319 SNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLNVV 378

Query: 372 PTEPRNRATRVASNRNAHQPLHH 393
           P   R+   +  +N +   P H+
Sbjct: 379 PN--RSSTKQAVANSSRSSPHHY 395

BLAST of MC04g0324 vs. TAIR 10
Match: AT4G35600.2 (Protein kinase superfamily protein )

HSP 1 Score: 408.7 bits (1049), Expect = 5.6e-114
Identity = 206/383 (53.79%), Postives = 279/383 (72.85%), Query Frame = 0

Query: 12  HTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQILPSPNLKEYSLA 71
           H+   T N  S  ++ +S T ++T S++ + S FS  S      +G++L SPNLK Y+  
Sbjct: 19  HSHAATTNNHSNGTEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNFL 78

Query: 72  ELKAATKNFRPEALLGEGGFGKVYKGWLEEK--GYGKRGNPMVIAVKKLKSDSMQGIEEW 131
           +LK ATKNF+P+++LG+GGFGKVY+GW++       + G+ M++A+K+L S+S+QG  EW
Sbjct: 79  DLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAEW 138

Query: 132 QSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAVTPLQWDTRL 191
           +SEV FLG LSH NLVKLLGYC ED + LL YEFM KGSLE+HLF R     P  WD R+
Sbjct: 139 RSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRI 198

Query: 192 KVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTR 251
           K+ IGAARGLAFLH+   +VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TTR
Sbjct: 199 KIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTR 258

Query: 252 VMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQENLLDWIKPYL 311
           +MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE+L+DW++P L
Sbjct: 259 IMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPEL 318

Query: 312 SERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETLEQIEATNEK 371
           S + R+K +MD  ++G+Y ++ A ++A++ L CIE + +NRP MKEVVE LE I+  N  
Sbjct: 319 SNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLNVV 378

Query: 372 PTEPRNRATRVASNRNAHQPLHH 393
           P   R+   +  +N +   P H+
Sbjct: 379 PN--RSSTKQAVANSSRSSPHHY 396

BLAST of MC04g0324 vs. TAIR 10
Match: AT1G76360.1 (Protein kinase superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.4e-112
Identity = 198/353 (56.09%), Postives = 260/353 (73.65%), Query Frame = 0

Query: 26  QTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQILPSPNLKEYSLAELKAATKNFRPEAL 85
           +T  Q  +  V N     +     G +A P    + +PNLK ++L ELK ATKNFRPE++
Sbjct: 109 ETPPQEKTRPVDNPPSKPVEKLGLGRKAVPPSGKIVTPNLKMFTLVELKTATKNFRPESV 168

Query: 86  LGEGGFGKVYKGWLEEK--GYGKRGNPMVIAVKKLKSDSMQGIEEWQSEVGFLGRLSHPN 145
           +GEGGFG+V+KGW++EK     + G  + +AVKK   DS QG+ EWQ EV FLG+  HPN
Sbjct: 169 IGEGGFGQVFKGWVDEKTLAPSRAGVGIPVAVKKSNPDSEQGLHEWQCEVRFLGKFHHPN 228

Query: 146 LVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAVTPLQWDTRLKVAIGAARGLAFLH 205
           LVKLLGYCWE++QFLL YE++ KGSLENHLF +G+   P  WDTRLK+AI AA+GL FLH
Sbjct: 229 LVKLLGYCWEENQFLLVYEYLPKGSLENHLFSKGAEALP--WDTRLKIAIEAAQGLTFLH 288

Query: 206 TSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTHGYAAPEYVT 265
            S++ VIYRDFKASNILLD ++ AKLSDFGLAK GP    SH+TTRVMGT GYAAPEY+ 
Sbjct: 289 NSEKSVIYRDFKASNILLDSNFHAKLSDFGLAKNGPINGFSHVTTRVMGTQGYAAPEYMA 348

Query: 266 TGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQENLLDWIKPYLSERRRLKNVMDFRL 325
           TGHLYV+SDVYGFGVVL+E+LTGLRALD NRP+ Q+NL++W KP L+++++++ +MD RL
Sbjct: 349 TGHLYVRSDVYGFGVVLLELLTGLRALDPNRPSAQQNLVEWAKPGLNQKKKVQKMMDPRL 408

Query: 326 EGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETLEQIEATNEKPTEPRNR 377
           E KYP  +  + A+L L+C+E + +NRP M +V+  LE +    ++P E R +
Sbjct: 409 EQKYPLLAVTKTAELILRCLEADPKNRPPMDDVLRELEVVRTIRDQPQEERRK 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SII64.3e-15167.73Probable serine/threonine-protein kinase PIX13 OS=Arabidopsis thaliana OX=3702 G... [more]
P274507.9e-11354.05Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q65XV81.1e-10951.80Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P465739.1e-10954.86Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
P432932.0e-10853.32Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
XP_022136188.11.09e-298100.00probable serine/threonine-protein kinase PIX13 [Momordica charantia][more]
XP_038886859.11.03e-27190.31probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886860... [more]
XP_008447841.11.89e-26589.37PREDICTED: probable serine/threonine-protein kinase Cx32, chloroplastic [Cucumis... [more]
XP_004154222.12.03e-26288.14probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_011658631.1... [more]
KAG6589052.11.08e-25485.96putative serine/threonine-protein kinase PIX13, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
A0A6J1C6Y95.30e-299100.00probable serine/threonine-protein kinase PIX13 OS=Momordica charantia OX=3673 GN... [more]
A0A5D3DI799.16e-26689.37Putative serine/threonine-protein kinase Cx32 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3BHT19.16e-26689.37probable serine/threonine-protein kinase Cx32, chloroplastic OS=Cucumis melo OX=... [more]
A0A6J1EKG22.13e-25485.71probable serine/threonine-protein kinase PIX13 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JQ742.48e-25385.96probable serine/threonine-protein kinase PIX13 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT2G17220.13.1e-15267.73Protein kinase superfamily protein [more]
AT2G17220.28.9e-15267.65Protein kinase superfamily protein [more]
AT4G35600.15.6e-11454.05Protein kinase superfamily protein [more]
AT4G35600.25.6e-11453.79Protein kinase superfamily protein [more]
AT1G76360.12.4e-11256.09Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 64..163
e-value: 1.9E-31
score: 110.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 164..388
e-value: 1.9E-60
score: 205.8
NoneNo IPR availablePIRSRPIRSR630616-1PIRSR630616-1coord: 8..294
e-value: 1.6E-21
score: 74.4
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 86..282
e-value: 6.9E-13
score: 45.7
NoneNo IPR availablePIRSRPIRSR000605-50PIRSR000605-50coord: 72..297
e-value: 3.0E-16
score: 57.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 365..413
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 376..390
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..35
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 10..392
NoneNo IPR availablePANTHERPTHR45621:SF169BNAA09G08980D PROTEINcoord: 10..392
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 86..363
e-value: 1.87483E-93
score: 280.313
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 84..360
e-value: 1.5E-45
score: 155.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 209..221
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 86..117
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 80..363
score: 37.940617
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 64..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0324.1MC04g0324.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity