MC04g0296 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0296
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPentatricopeptide repeat-containing protein
LocationMC04: 2342749 .. 2343825 (-)
RNA-Seq ExpressionMC04g0296
SyntenyMC04g0296
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGCAATTGCAATCTCGCCTTCGAATGTCGAAGATTGTGTCTTCATCAATTTGGAAGTGCGAAAGGCAGCTTCCTCTGCTCCACGATTCACTGCGAACGCAGGATTTCTCGCTCTTGCCGACGCAATCTCGCTGTTTCAGCTCCAATTCCATGGACGACGATTGGGCCATCTCAAATTCCGAGCCGAGGAGCAACCCGCCGCACCGGCGTTCTCCTCCGGATTCCCGCGAACCTCGTCGAGTTCCTAAATTCTCCGATGGAGTATCTGCTTCTTACCCTGGGGATGATCGGAACCGACGGATTAATAAACGTTCGGAAGGTTCCTCGTTTCGATTTGATAATGAAGGCGGCATTTCGCGGCAAAGGAGTGAGTTTCTGTCTCAGAAGGATTTTAGCTTTCTTGAAAAGTTCAAGCTGAATACTGATAATCAGAGTAGTAGCAAAGAGAAGACCGAGCAGAGCTCTGCTCCTCGGTTTTCTGAATCTGTCCAGGAGAAGCAGTCGCAACCGCAGCGTCCTCCTGAAGCCGATGAGATATTCAAGAAAATGAAGGAGACGGGTCTCATTCCTAATGCTGTCGCCATGCTTGATGGGCTGTGCAAAGATGGGCTTATCCAAGAAGCAATGAAACTCTTTGGTTTGATTCGTGAGAAGGGTACAATTCCAGAAGTTGTGATTTACACCGCTGTAGTTGATGGGTTTTGCAAGGCCGAGAAGCTTGATGAAGCCATCAGGATTTTCAGAAAAATGCAGAAAAATGGCATTTCTCCTAATGCTTTCAGTTTTGGCGTCTTGATACAGGGACTGTACAAATGCAATAAATTAGAGGAAGCCATAGGATTTTGCATTGAGATGTTAGAATCTGGGCATTCTCCAAATGTAACCACTCTTGTTGGCCTAGTTGACGAGTTATGCAAAGAAAGGGGAACGGAAGAAGCTCGGAGTGTCATAGAAACCTTAAAACAAAAAGGGTTCTTGCTGAATGAGAAAGCTTTAAGAGAATTTCTGAACAAAAGAGCCCCCTTTTCACCAAATATCTGGGAAGCAATCTTTGGGAAAAAAACGAAAGAATTTTTC

mRNA sequence

TTGCAATTGCAATCTCGCCTTCGAATGTCGAAGATTGTGTCTTCATCAATTTGGAAGTGCGAAAGGCAGCTTCCTCTGCTCCACGATTCACTGCGAACGCAGGATTTCTCGCTCTTGCCGACGCAATCTCGCTGTTTCAGCTCCAATTCCATGGACGACGATTGGGCCATCTCAAATTCCGAGCCGAGGAGCAACCCGCCGCACCGGCGTTCTCCTCCGGATTCCCGCGAACCTCGTCGAGTTCCTAAATTCTCCGATGGAGTATCTGCTTCTTACCCTGGGGATGATCGGAACCGACGGATTAATAAACGTTCGGAAGGTTCCTCGTTTCGATTTGATAATGAAGGCGGCATTTCGCGGCAAAGGAGTGAGTTTCTGTCTCAGAAGGATTTTAGCTTTCTTGAAAAGTTCAAGCTGAATACTGATAATCAGAGTAGTAGCAAAGAGAAGACCGAGCAGAGCTCTGCTCCTCGGTTTTCTGAATCTGTCCAGGAGAAGCAGTCGCAACCGCAGCGTCCTCCTGAAGCCGATGAGATATTCAAGAAAATGAAGGAGACGGGTCTCATTCCTAATGCTGTCGCCATGCTTGATGGGCTGTGCAAAGATGGGCTTATCCAAGAAGCAATGAAACTCTTTGGTTTGATTCGTGAGAAGGGTACAATTCCAGAAGTTGTGATTTACACCGCTGTAGTTGATGGGTTTTGCAAGGCCGAGAAGCTTGATGAAGCCATCAGGATTTTCAGAAAAATGCAGAAAAATGGCATTTCTCCTAATGCTTTCAGTTTTGGCGTCTTGATACAGGGACTGTACAAATGCAATAAATTAGAGGAAGCCATAGGATTTTGCATTGAGATGTTAGAATCTGGGCATTCTCCAAATGTAACCACTCTTGTTGGCCTAGTTGACGAGTTATGCAAAGAAAGGGGAACGGAAGAAGCTCGGAGTGTCATAGAAACCTTAAAACAAAAAGGGTTCTTGCTGAATGAGAAAGCTTTAAGAGAATTTCTGAACAAAAGAGCCCCCTTTTCACCAAATATCTGGGAAGCAATCTTTGGGAAAAAAACGAAAGAATTTTTC

Coding sequence (CDS)

TTGCAATTGCAATCTCGCCTTCGAATGTCGAAGATTGTGTCTTCATCAATTTGGAAGTGCGAAAGGCAGCTTCCTCTGCTCCACGATTCACTGCGAACGCAGGATTTCTCGCTCTTGCCGACGCAATCTCGCTGTTTCAGCTCCAATTCCATGGACGACGATTGGGCCATCTCAAATTCCGAGCCGAGGAGCAACCCGCCGCACCGGCGTTCTCCTCCGGATTCCCGCGAACCTCGTCGAGTTCCTAAATTCTCCGATGGAGTATCTGCTTCTTACCCTGGGGATGATCGGAACCGACGGATTAATAAACGTTCGGAAGGTTCCTCGTTTCGATTTGATAATGAAGGCGGCATTTCGCGGCAAAGGAGTGAGTTTCTGTCTCAGAAGGATTTTAGCTTTCTTGAAAAGTTCAAGCTGAATACTGATAATCAGAGTAGTAGCAAAGAGAAGACCGAGCAGAGCTCTGCTCCTCGGTTTTCTGAATCTGTCCAGGAGAAGCAGTCGCAACCGCAGCGTCCTCCTGAAGCCGATGAGATATTCAAGAAAATGAAGGAGACGGGTCTCATTCCTAATGCTGTCGCCATGCTTGATGGGCTGTGCAAAGATGGGCTTATCCAAGAAGCAATGAAACTCTTTGGTTTGATTCGTGAGAAGGGTACAATTCCAGAAGTTGTGATTTACACCGCTGTAGTTGATGGGTTTTGCAAGGCCGAGAAGCTTGATGAAGCCATCAGGATTTTCAGAAAAATGCAGAAAAATGGCATTTCTCCTAATGCTTTCAGTTTTGGCGTCTTGATACAGGGACTGTACAAATGCAATAAATTAGAGGAAGCCATAGGATTTTGCATTGAGATGTTAGAATCTGGGCATTCTCCAAATGTAACCACTCTTGTTGGCCTAGTTGACGAGTTATGCAAAGAAAGGGGAACGGAAGAAGCTCGGAGTGTCATAGAAACCTTAAAACAAAAAGGGTTCTTGCTGAATGAGAAAGCTTTAAGAGAATTTCTGAACAAAAGAGCCCCCTTTTCACCAAATATCTGGGAAGCAATCTTTGGGAAAAAAACGAAAGAATTTTTC

Protein sequence

LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNSEPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISRQRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF
Homology
BLAST of MC04g0296 vs. ExPASy Swiss-Prot
Match: Q9SZL5 (Pentatricopeptide repeat-containing protein At4g38150 OS=Arabidopsis thaliana OX=3702 GN=At4g38150 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 4.0e-76
Identity = 147/229 (64.19%), Postives = 174/229 (75.98%), Query Frame = 0

Query: 130 DFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPE-ADEIFKKMKETGL 189
           D  FLE+FKL  +  S    K EQ               +P  PPE +DEIFKKMKE GL
Sbjct: 83  DDGFLEQFKLGVNQDSRETPKPEQ------------YPQEPLPPPEDSDEIFKKMKEGGL 142

Query: 190 IPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKLDEAIRIFR 249
           IPNAVAMLDGLCKDGL+QEAMKLFGL+R+KGTIPEVVIYTAVV+ FCKA K+++A RIFR
Sbjct: 143 IPNAVAMLDGLCKDGLVQEAMKLFGLMRDKGTIPEVVIYTAVVEAFCKAHKIEDAKRIFR 202

Query: 250 KMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGLVDELCKER 309
           KMQ NGI+PNAFS+GVL+QGLY CN L++A+ FC EMLESGHSPNV T V LVD LC+ +
Sbjct: 203 KMQNNGIAPNAFSYGVLVQGLYNCNMLDDAVAFCSEMLESGHSPNVPTFVELVDALCRVK 262

Query: 310 GTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKE 358
           G E+A+S I+TL QKGF +N KA++EF++KRAPF    WEAIF KK  E
Sbjct: 263 GVEQAQSAIDTLNQKGFAVNVKAVKEFMDKRAPFPSLAWEAIFKKKPTE 299

BLAST of MC04g0296 vs. ExPASy Swiss-Prot
Match: Q9LPX2 (Pentatricopeptide repeat-containing protein At1g12775, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g12775 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.1e-22
Identity = 57/152 (37.50%), Postives = 84/152 (55.26%), Query Frame = 0

Query: 176 ADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVD 235
           A E+ +KM+E  +  +AV    ++DGLCKDG +  A  LF  +  KG   +++ Y  ++ 
Sbjct: 247 AMELLRKMEERNIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIKGFKADIITYNTLIG 306

Query: 236 GFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSP 295
           GFC A + D+  ++ R M K  ISPN  +F VLI    K  KL EA     EM++ G +P
Sbjct: 307 GFCNAGRWDDGAKLLRDMIKRKISPNVVTFSVLIDSFVKEGKLREADQLLKEMMQRGIAP 366

Query: 296 NVTTLVGLVDELCKERGTEEARSVIETLKQKG 325
           N  T   L+D  CKE   EEA  +++ +  KG
Sbjct: 367 NTITYNSLIDGFCKENRLEEAIQMVDLMISKG 398

BLAST of MC04g0296 vs. ExPASy Swiss-Prot
Match: Q0WVK7 (Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g05670 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.3e-22
Identity = 59/179 (32.96%), Postives = 96/179 (53.63%), Query Frame = 0

Query: 175 EADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV 234
           EA+E F +M   G++P+ V    ++DG CK G I+ A K F  +  +   P+V+ YTA++
Sbjct: 334 EAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGDIRAASKFFYEMHSRDITPDVLTYTAII 393

Query: 235 DGFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHS 294
            GFC+   + EA ++F +M   G+ P++ +F  LI G  K   +++A      M+++G S
Sbjct: 394 SGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRVHNHMIQAGCS 453

Query: 295 PNVTTLVGLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAI 351
           PNV T   L+D LCKE   + A  ++  + + G   N       +N     S NI EA+
Sbjct: 454 PNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCK-SGNIEEAV 511

BLAST of MC04g0296 vs. ExPASy Swiss-Prot
Match: Q9ASZ8 (Pentatricopeptide repeat-containing protein At1g12620 OS=Arabidopsis thaliana OX=3702 GN=At1g12620 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.6e-21
Identity = 56/165 (33.94%), Postives = 89/165 (53.94%), Query Frame = 0

Query: 176 ADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVD 235
           A E+ +KM+E  +  +AV    ++DGLCKDG +  A  LF  +  KG   +++IYT ++ 
Sbjct: 231 AMELLRKMEERKIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIKGFKADIIIYTTLIR 290

Query: 236 GFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSP 295
           GFC A + D+  ++ R M K  I+P+  +F  LI    K  KL EA     EM++ G SP
Sbjct: 291 GFCYAGRWDDGAKLLRDMIKRKITPDVVAFSALIDCFVKEGKLREAEELHKEMIQRGISP 350

Query: 296 NVTTLVGLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLN 338
           +  T   L+D  CKE   ++A  +++ +  KG   N +     +N
Sbjct: 351 DTVTYTSLIDGFCKENQLDKANHMLDLMVSKGCGPNIRTFNILIN 395

BLAST of MC04g0296 vs. ExPASy Swiss-Prot
Match: Q9FIT7 (Pentatricopeptide repeat-containing protein At5g61990, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g61990 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 8.1e-21
Identity = 51/152 (33.55%), Postives = 90/152 (59.21%), Query Frame = 0

Query: 176 ADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVD 235
           AD+  K+M+E G++PN V    +++  CK G + EA   +  + ++G + +   YT +++
Sbjct: 541 ADKYVKEMRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTVLMN 600

Query: 236 GFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSP 295
           G  K +K+D+A  IFR+M+  GI+P+ FS+GVLI G  K   +++A     EM+E G +P
Sbjct: 601 GLFKNDKVDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTP 660

Query: 296 NVTTLVGLVDELCKERGTEEARSVIETLKQKG 325
           NV     L+   C+    E+A+ +++ +  KG
Sbjct: 661 NVIIYNMLLGGFCRSGEIEKAKELLDEMSVKG 692

BLAST of MC04g0296 vs. NCBI nr
Match: XP_022136091.1 (pentatricopeptide repeat-containing protein At4g38150 [Momordica charantia] >XP_022136092.1 pentatricopeptide repeat-containing protein At4g38150 [Momordica charantia])

HSP 1 Score: 694 bits (1792), Expect = 1.16e-251
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS
Sbjct: 6   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR
Sbjct: 66  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
           QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF
Sbjct: 126 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL
Sbjct: 186 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL
Sbjct: 246 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF
Sbjct: 306 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 364

BLAST of MC04g0296 vs. NCBI nr
Match: XP_022969144.1 (pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita maxima] >XP_022969146.1 pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita maxima])

HSP 1 Score: 555 bits (1431), Expect = 1.14e-196
Identity = 288/359 (80.22%), Postives = 316/359 (88.02%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           +QLQSRLR+SKI+ S+I   ER  PLLHD LRT D SL P +SRCFSSNSMDDDW  S S
Sbjct: 6   MQLQSRLRVSKILFSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDWNFSRS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRS+PP RRSPPD RE RRVPKF+  VSA YP D+RN+R N+  EGS  RF NEG +SR
Sbjct: 66  EPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHWEGSESRFANEGPMSR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
           +RSE LSQKDFSFLEKFKLNTDN S+S EKTE SS+ +FSES+Q KQSQPQ PPEADEIF
Sbjct: 126 RRSESLSQKDFSFLEKFKLNTDNLSTSTEKTEPSSSAQFSESMQGKQSQPQCPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           +KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVVDGFCKAE L
Sbjct: 186 RKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFCKAENL 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQ NGISPNAFS+GVLIQGLYKC KLE+A+ FCIEMLESGHSPN+TT VGL
Sbjct: 246 DEAIRIFRKMQNNGISPNAFSYGVLIQGLYKCKKLEDAVSFCIEMLESGHSPNLTTFVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           +DELCKE+G +EA +VIETLKQKGFL+NEKALREFLNKRAPFSP+IWE IFGKKTKEFF
Sbjct: 306 IDELCKEKGVDEAHTVIETLKQKGFLINEKALREFLNKRAPFSPHIWELIFGKKTKEFF 364

BLAST of MC04g0296 vs. NCBI nr
Match: XP_023554525.1 (pentatricopeptide repeat-containing protein At4g38150-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554526.1 pentatricopeptide repeat-containing protein At4g38150-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 553 bits (1424), Expect = 1.32e-195
Identity = 286/359 (79.67%), Postives = 318/359 (88.58%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           +QLQSRLR+SKI+SS+I   ER  PLLHD LRT D SL P +SRCFSSNSMDDDW  S S
Sbjct: 6   MQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDWNFSRS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRS+PP RRSPPD RE RRVPKF+   SA YP D+RN+R N+ SEGSS RF NEG +SR
Sbjct: 66  EPRSSPPQRRSPPDPREARRVPKFAGEGSAPYPEDNRNQRFNRHSEGSSSRFANEGPMSR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
           +R+E LSQKDFSFLEKFKLNTDNQS+S EK E SS+ +FSES+Q +QSQPQ PPEADEIF
Sbjct: 126 RRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQFSESMQGEQSQPQCPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           +KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVVDGFCKAEKL
Sbjct: 186 RKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFCKAEKL 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQ NGISPNAFS+G+LIQGLYK  +LE+A+ FCIEMLESGHSPN+TT VGL
Sbjct: 246 DEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCIEMLESGHSPNLTTFVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           +DELCKE+G +EA +VIETLKQKGFL+NEKALREFLNKRAPFSP+IWE IFGKKTKEFF
Sbjct: 306 IDELCKEKGEDEAHTVIETLKQKGFLINEKALREFLNKRAPFSPHIWELIFGKKTKEFF 364

BLAST of MC04g0296 vs. NCBI nr
Match: KAG7011839.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 551 bits (1420), Expect = 5.38e-195
Identity = 285/359 (79.39%), Postives = 315/359 (87.74%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           +QLQSRLR+SKI+SS+I   ER  PLLHD LRT D SL P +SRCFSSNSMDDDW  S S
Sbjct: 6   MQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDWNFSKS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRS+PP RRSPPD RE RRVPKF+  VSA YP D+RN+R N+ SEGSS RF NEG +SR
Sbjct: 66  EPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHSEGSSSRFANEGPMSR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
            R+E LSQKDFSFLEKFKLNTDNQS+S EK E SS+ +FSES+Q KQSQPQ PPEADEIF
Sbjct: 126 PRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQFSESMQGKQSQPQCPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           +KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVVDGFCKAEK 
Sbjct: 186 RKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFCKAEKF 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQ NGISPNAFS+G+LIQGLYK  +LE+A+ FC EMLESGHSPN+TT VGL
Sbjct: 246 DEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCFEMLESGHSPNLTTFVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           +DELCKE+G +EA +VIETLKQKGFL+NEK LREFLNKRAPFSP+IWE IFGKKTKEFF
Sbjct: 306 IDELCKEKGVDEAHTVIETLKQKGFLINEKDLREFLNKRAPFSPHIWELIFGKKTKEFF 364

BLAST of MC04g0296 vs. NCBI nr
Match: XP_022952706.1 (pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita moschata] >XP_022952707.1 pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita moschata])

HSP 1 Score: 550 bits (1416), Expect = 2.19e-194
Identity = 284/359 (79.11%), Postives = 316/359 (88.02%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           +QLQSRLR+SKI+SS+I   ER  PLLHD LRT D SL P +SRCFSSNSMDDDW  S S
Sbjct: 6   MQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDWNFSRS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRS+PP RRSPPD RE RRVPKF+  VSA YP D+RN+R N+ SEGSS RF N+G +SR
Sbjct: 66  EPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHSEGSSSRFANDGPMSR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
            R+E LSQKDFSFLEKFKLNTDNQS+S EK E SS+ + SES+Q KQSQPQ PPEADEIF
Sbjct: 126 PRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQISESMQGKQSQPQCPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           +KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVVDGFCKAEKL
Sbjct: 186 RKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFCKAEKL 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQ NGISPNAFS+G+LIQGLYK  +LE+A+ FCIEMLESGHSPN+TT VGL
Sbjct: 246 DEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCIEMLESGHSPNLTTFVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           +DELCKE+G +EA +VIETLKQKGFL+NEK LR+FLNKRAPFSP+IWE IFGKKTKEFF
Sbjct: 306 IDELCKEKGVDEAHTVIETLKQKGFLINEKDLRDFLNKRAPFSPHIWELIFGKKTKEFF 364

BLAST of MC04g0296 vs. ExPASy TrEMBL
Match: A0A6J1C2J3 (pentatricopeptide repeat-containing protein At4g38150 OS=Momordica charantia OX=3673 GN=LOC111007869 PE=4 SV=1)

HSP 1 Score: 694 bits (1792), Expect = 5.62e-252
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS
Sbjct: 6   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR
Sbjct: 66  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
           QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF
Sbjct: 126 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL
Sbjct: 186 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL
Sbjct: 246 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF
Sbjct: 306 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 364

BLAST of MC04g0296 vs. ExPASy TrEMBL
Match: A0A6J1I1Q2 (pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita maxima OX=3661 GN=LOC111468227 PE=4 SV=1)

HSP 1 Score: 555 bits (1431), Expect = 5.51e-197
Identity = 288/359 (80.22%), Postives = 316/359 (88.02%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           +QLQSRLR+SKI+ S+I   ER  PLLHD LRT D SL P +SRCFSSNSMDDDW  S S
Sbjct: 6   MQLQSRLRVSKILFSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDWNFSRS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRS+PP RRSPPD RE RRVPKF+  VSA YP D+RN+R N+  EGS  RF NEG +SR
Sbjct: 66  EPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHWEGSESRFANEGPMSR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
           +RSE LSQKDFSFLEKFKLNTDN S+S EKTE SS+ +FSES+Q KQSQPQ PPEADEIF
Sbjct: 126 RRSESLSQKDFSFLEKFKLNTDNLSTSTEKTEPSSSAQFSESMQGKQSQPQCPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           +KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVVDGFCKAE L
Sbjct: 186 RKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFCKAENL 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQ NGISPNAFS+GVLIQGLYKC KLE+A+ FCIEMLESGHSPN+TT VGL
Sbjct: 246 DEAIRIFRKMQNNGISPNAFSYGVLIQGLYKCKKLEDAVSFCIEMLESGHSPNLTTFVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           +DELCKE+G +EA +VIETLKQKGFL+NEKALREFLNKRAPFSP+IWE IFGKKTKEFF
Sbjct: 306 IDELCKEKGVDEAHTVIETLKQKGFLINEKALREFLNKRAPFSPHIWELIFGKKTKEFF 364

BLAST of MC04g0296 vs. ExPASy TrEMBL
Match: A0A6J1GKZ0 (pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita moschata OX=3662 GN=LOC111455321 PE=4 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 1.06e-194
Identity = 284/359 (79.11%), Postives = 316/359 (88.02%), Query Frame = 0

Query: 1   LQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNS 60
           +QLQSRLR+SKI+SS+I   ER  PLLHD LRT D SL P +SRCFSSNSMDDDW  S S
Sbjct: 6   MQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDWNFSRS 65

Query: 61  EPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISR 120
           EPRS+PP RRSPPD RE RRVPKF+  VSA YP D+RN+R N+ SEGSS RF N+G +SR
Sbjct: 66  EPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHSEGSSSRFANDGPMSR 125

Query: 121 QRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPEADEIF 180
            R+E LSQKDFSFLEKFKLNTDNQS+S EK E SS+ + SES+Q KQSQPQ PPEADEIF
Sbjct: 126 PRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQISESMQGKQSQPQCPPEADEIF 185

Query: 181 KKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKL 240
           +KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVVDGFCKAEKL
Sbjct: 186 RKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFCKAEKL 245

Query: 241 DEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGL 300
           DEAIRIFRKMQ NGISPNAFS+G+LIQGLYK  +LE+A+ FCIEMLESGHSPN+TT VGL
Sbjct: 246 DEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCIEMLESGHSPNLTTFVGL 305

Query: 301 VDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           +DELCKE+G +EA +VIETLKQKGFL+NEK LR+FLNKRAPFSP+IWE IFGKKTKEFF
Sbjct: 306 IDELCKEKGVDEAHTVIETLKQKGFLINEKDLRDFLNKRAPFSPHIWELIFGKKTKEFF 364

BLAST of MC04g0296 vs. ExPASy TrEMBL
Match: A0A0A0K6C0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G029950 PE=4 SV=1)

HSP 1 Score: 498 bits (1282), Expect = 2.24e-174
Identity = 268/360 (74.44%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 2   QLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNSE 61
           QLQSRL++SKI+SS+I K E   PLLH+ LRT DFSL P QSR F+SNSMDDD A S+SE
Sbjct: 5   QLQSRLQVSKILSSTIRKWEIDFPLLHNPLRTPDFSLYPAQSRRFASNSMDDDHAFSDSE 64

Query: 62  PRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISRQ 121
           P + PP RR PPD RE RRVP+   GV+ASY  D+RN+R N+ SEGSS RF NEG  S Q
Sbjct: 65  PINTPPQRRFPPDHREARRVPRG--GVTASY--DNRNQRFNRHSEGSSSRFTNEGSTSPQ 124

Query: 122 RSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQ--SSAPRFSESVQEKQSQPQRPPEADEI 181
           RSE LSQKDFSFLEKFKLNTDNQSS KEKTE+  SSAP     ++++QS+PQRPPEADEI
Sbjct: 125 RSESLSQKDFSFLEKFKLNTDNQSSGKEKTEENSSSAPVSESMLEKQQSEPQRPPEADEI 184

Query: 182 FKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEK 241
           F KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEK
Sbjct: 185 FGKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEK 244

Query: 242 LDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVG 301
            DEAIRIFRKMQ NGI PNAFSFGVLIQGLYKC KL++A+ FC EMLESGH PN+TT VG
Sbjct: 245 FDEAIRIFRKMQHNGIPPNAFSFGVLIQGLYKCKKLDDAVAFCNEMLESGHLPNLTTFVG 304

Query: 302 LVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEFF 359
           L+D LC E+G +EA SV+ET KQKGFL++EKALRE LNKRAPFSP+IW+  FG +   FF
Sbjct: 305 LIDALCNEKGVDEAHSVVETFKQKGFLIDEKALREVLNKRAPFSPDIWKVFFGNRKPPFF 360

BLAST of MC04g0296 vs. ExPASy TrEMBL
Match: A0A1S3CFW0 (pentatricopeptide repeat-containing protein At4g38150 OS=Cucumis melo OX=3656 GN=LOC103500481 PE=4 SV=1)

HSP 1 Score: 494 bits (1273), Expect = 5.25e-173
Identity = 272/361 (75.35%), Postives = 299/361 (82.83%), Query Frame = 0

Query: 2   QLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDWAISNSE 61
           Q QSRL++SKI+SSSI K E   PLLH+ LRT DFSL P  SR FSSNSMDDD A S SE
Sbjct: 5   QFQSRLQVSKILSSSIRKWEIDFPLLHNPLRTPDFSLSPAPSRRFSSNSMDDDRAFSESE 64

Query: 62  PRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNEGGISRQ 121
           PR+ PP RR PPD RE RRVP+   GV+ SY  D+RN+R N+ SEGSS RF NEG  SRQ
Sbjct: 65  PRNTPPQRRFPPDHREARRVPRG--GVTDSY--DNRNQRFNRHSEGSSSRFTNEGSTSRQ 124

Query: 122 RSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQ--SSAPRFSESVQEKQ-SQPQRPPEADE 181
           RSE LSQKDFSFLEKFKLNTDNQSS  EKTE+  SSAP  SES+QEKQ SQ QRPP ADE
Sbjct: 125 RSESLSQKDFSFLEKFKLNTDNQSSGNEKTEENSSSAP-VSESMQEKQQSQHQRPPAADE 184

Query: 182 IFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAE 241
           IF+KMKE+GLIPNAVAMLDGLCKDGLIQEAMKLF LIREKGTIPEVVIYTAVVDGFCKAE
Sbjct: 185 IFRKMKESGLIPNAVAMLDGLCKDGLIQEAMKLFALIREKGTIPEVVIYTAVVDGFCKAE 244

Query: 242 KLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLV 301
           K DEAIRIFRKMQ  GISPNAFSFGVLIQGLYKC KL++A+ FC EMLESGH PN+TT V
Sbjct: 245 KFDEAIRIFRKMQNKGISPNAFSFGVLIQGLYKCKKLDDAVAFCNEMLESGHLPNLTTFV 304

Query: 302 GLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKEF 359
           GL+D LC E+G +EA +V+ET KQKGFL++EKALREFLNKRAPFSP+IW+  FG K   F
Sbjct: 305 GLIDVLCSEKGVDEAHNVVETFKQKGFLIDEKALREFLNKRAPFSPDIWKVFFGNKKAPF 360

BLAST of MC04g0296 vs. TAIR 10
Match: AT4G38150.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.8e-77
Identity = 147/229 (64.19%), Postives = 174/229 (75.98%), Query Frame = 0

Query: 130 DFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPE-ADEIFKKMKETGL 189
           D  FLE+FKL  +  S    K EQ               +P  PPE +DEIFKKMKE GL
Sbjct: 83  DDGFLEQFKLGVNQDSRETPKPEQ------------YPQEPLPPPEDSDEIFKKMKEGGL 142

Query: 190 IPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKLDEAIRIFR 249
           IPNAVAMLDGLCKDGL+QEAMKLFGL+R+KGTIPEVVIYTAVV+ FCKA K+++A RIFR
Sbjct: 143 IPNAVAMLDGLCKDGLVQEAMKLFGLMRDKGTIPEVVIYTAVVEAFCKAHKIEDAKRIFR 202

Query: 250 KMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGLVDELCKER 309
           KMQ NGI+PNAFS+GVL+QGLY CN L++A+ FC EMLESGHSPNV T V LVD LC+ +
Sbjct: 203 KMQNNGIAPNAFSYGVLVQGLYNCNMLDDAVAFCSEMLESGHSPNVPTFVELVDALCRVK 262

Query: 310 GTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKE 358
           G E+A+S I+TL QKGF +N KA++EF++KRAPF    WEAIF KK  E
Sbjct: 263 GVEQAQSAIDTLNQKGFAVNVKAVKEFMDKRAPFPSLAWEAIFKKKPTE 299

BLAST of MC04g0296 vs. TAIR 10
Match: AT4G38150.2 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.8e-77
Identity = 147/229 (64.19%), Postives = 174/229 (75.98%), Query Frame = 0

Query: 130 DFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPE-ADEIFKKMKETGL 189
           D  FLE+FKL  +  S    K EQ               +P  PPE +DEIFKKMKE GL
Sbjct: 83  DDGFLEQFKLGVNQDSRETPKPEQ------------YPQEPLPPPEDSDEIFKKMKEGGL 142

Query: 190 IPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFCKAEKLDEAIRIFR 249
           IPNAVAMLDGLCKDGL+QEAMKLFGL+R+KGTIPEVVIYTAVV+ FCKA K+++A RIFR
Sbjct: 143 IPNAVAMLDGLCKDGLVQEAMKLFGLMRDKGTIPEVVIYTAVVEAFCKAHKIEDAKRIFR 202

Query: 250 KMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVTTLVGLVDELCKER 309
           KMQ NGI+PNAFS+GVL+QGLY CN L++A+ FC EMLESGHSPNV T V LVD LC+ +
Sbjct: 203 KMQNNGIAPNAFSYGVLVQGLYNCNMLDDAVAFCSEMLESGHSPNVPTFVELVDALCRVK 262

Query: 310 GTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAIFGKKTKE 358
           G E+A+S I+TL QKGF +N KA++EF++KRAPF    WEAIF KK  E
Sbjct: 263 GVEQAQSAIDTLNQKGFAVNVKAVKEFMDKRAPFPSLAWEAIFKKKPTE 299

BLAST of MC04g0296 vs. TAIR 10
Match: AT5G03560.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.8e-28
Identity = 98/313 (31.31%), Postives = 157/313 (50.16%), Query Frame = 0

Query: 58  SNSEPRSNPPHRRSPPDSREPRRVPKFSDGVSASY----PGDDRNRR---INKRSEGSSF 117
           S+S   S+P    +    +E +   +FS  + A +    P + R RR    +KR+  S F
Sbjct: 53  SSSSWWSSPDDLTASSKRKEKKTTERFSAVIDAVHDRKLPPELRGRRDFVRSKRNSNSLF 112

Query: 118 RFDNEGGISRQRSEFLSQKDFSFLEKFKLNTDNQSSSKE--------KTEQSSAPRFSES 177
            +   G  +RQ S     K  +    F L+ D+     E        K E    P    S
Sbjct: 113 PWFCGGVFARQFSS--ETKRVNTKVNFSLSDDDSDEETETQVTEDSKKQEPPPPPYDPFS 172

Query: 178 VQEKQSQPQRPPEADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIP 237
            +    +P+ P    EIF KM+  G    AV M D L KDG   EA++LF  I++K  +P
Sbjct: 173 KKPAIEEPEDPKNLQEIFHKMRTEGFTNEAVKMFDALSKDGRTHEALELFSQIKDKNRMP 232

Query: 238 EVVIYTAVVDGFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKL-EEAIGF 297
           +VV +TA+V+ +  A +  E +++F +M  +G+SPNA+++ VLI+GL    K  ++A  +
Sbjct: 233 DVVAHTAIVEAYANAGQAKETLKVFMRMLASGVSPNAYTYSVLIKGLAADGKTHKDAKKY 292

Query: 298 CIEMLESGHSPNVTTLVGLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLN-KRA 354
            +EM+ +G SPN  T   + +   +E   E AR +++ +K KGF+ +EKA+RE L  KR 
Sbjct: 293 LLEMMGNGMSPNAATYTAVFEAFVREGKEESARELLQEMKGKGFVPDEKAVREALEYKRG 352

BLAST of MC04g0296 vs. TAIR 10
Match: AT1G12775.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 8.0e-24
Identity = 57/152 (37.50%), Postives = 84/152 (55.26%), Query Frame = 0

Query: 176 ADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVD 235
           A E+ +KM+E  +  +AV    ++DGLCKDG +  A  LF  +  KG   +++ Y  ++ 
Sbjct: 247 AMELLRKMEERNIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIKGFKADIITYNTLIG 306

Query: 236 GFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSP 295
           GFC A + D+  ++ R M K  ISPN  +F VLI    K  KL EA     EM++ G +P
Sbjct: 307 GFCNAGRWDDGAKLLRDMIKRKISPNVVTFSVLIDSFVKEGKLREADQLLKEMMQRGIAP 366

Query: 296 NVTTLVGLVDELCKERGTEEARSVIETLKQKG 325
           N  T   L+D  CKE   EEA  +++ +  KG
Sbjct: 367 NTITYNSLIDGFCKENRLEEAIQMVDLMISKG 398

BLAST of MC04g0296 vs. TAIR 10
Match: AT1G05670.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 5.2e-23
Identity = 59/179 (32.96%), Postives = 96/179 (53.63%), Query Frame = 0

Query: 175 EADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV 234
           EA+E F +M   G++P+ V    ++DG CK G I+ A K F  +  +   P+V+ YTA++
Sbjct: 334 EAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGDIRAASKFFYEMHSRDITPDVLTYTAII 393

Query: 235 DGFCKAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHS 294
            GFC+   + EA ++F +M   G+ P++ +F  LI G  K   +++A      M+++G S
Sbjct: 394 SGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRVHNHMIQAGCS 453

Query: 295 PNVTTLVGLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWEAI 351
           PNV T   L+D LCKE   + A  ++  + + G   N       +N     S NI EA+
Sbjct: 454 PNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCK-SGNIEEAV 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZL54.0e-7664.19Pentatricopeptide repeat-containing protein At4g38150 OS=Arabidopsis thaliana OX... [more]
Q9LPX21.1e-2237.50Pentatricopeptide repeat-containing protein At1g12775, mitochondrial OS=Arabidop... [more]
Q0WVK77.3e-2232.96Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidop... [more]
Q9ASZ83.6e-2133.94Pentatricopeptide repeat-containing protein At1g12620 OS=Arabidopsis thaliana OX... [more]
Q9FIT78.1e-2133.55Pentatricopeptide repeat-containing protein At5g61990, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
XP_022136091.11.16e-251100.00pentatricopeptide repeat-containing protein At4g38150 [Momordica charantia] >XP_... [more]
XP_022969144.11.14e-19680.22pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita maxima] >X... [more]
XP_023554525.11.32e-19579.67pentatricopeptide repeat-containing protein At4g38150-like isoform X1 [Cucurbita... [more]
KAG7011839.15.38e-19579.39Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_022952706.12.19e-19479.11pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita moschata] ... [more]
Match NameE-valueIdentityDescription
A0A6J1C2J35.62e-252100.00pentatricopeptide repeat-containing protein At4g38150 OS=Momordica charantia OX=... [more]
A0A6J1I1Q25.51e-19780.22pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita maxima O... [more]
A0A6J1GKZ01.06e-19479.11pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita moschata... [more]
A0A0A0K6C02.24e-17474.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G029950 PE=4 SV=1[more]
A0A1S3CFW05.25e-17375.35pentatricopeptide repeat-containing protein At4g38150 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT4G38150.12.8e-7764.19Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G38150.22.8e-7764.19Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G03560.21.8e-2831.31Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G12775.18.0e-2437.50Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G05670.15.2e-2332.96Pentatricopeptide repeat (PPR-like) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 194..340
e-value: 8.7E-32
score: 111.9
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 261..294
e-value: 2.6E-4
score: 18.9
coord: 225..259
e-value: 8.3E-11
score: 39.4
coord: 194..222
e-value: 7.7E-5
score: 20.6
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 194..219
e-value: 5.7E-5
score: 23.1
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 222..271
e-value: 6.0E-16
score: 58.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 258..292
score: 10.314641
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 223..257
score: 13.296114
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 188..222
score: 8.582755
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..175
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..65
NoneNo IPR availablePANTHERPTHR45613:SF39PPR CONTAINING PLANT-LIKE PROTEINcoord: 52..357
NoneNo IPR availablePANTHERPTHR45613PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 52..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0296.1MC04g0296.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding