MC04g0288 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0288 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 1.3e-286
Identity = 492/777 (63.32%), Postives = 599/777 (77.09%), Query Frame = 0

Query: 15  MKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGG---REPADIVAN 74
           MK T++   F  LLLL L  + S  +L+  S    SYVVY GAHSH G    +  D V  
Sbjct: 1   MKLTHN---FSFLLLLLLVHMSSKHILA--SKDSSSYVVYFGAHSHVGEITEDAMDRVKE 60

Query: 75  SHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK 134
           +H++FL  F   S E   D I YSYT++INGFAA L+ D+A +++KH  VVSVF N+  K
Sbjct: 61  THYDFLGSF-TGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALK 120

Query: 135 LHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSR 194
           LHTTRSW+F+GLE N  + S SIW+KARFGEDTII NLDTG WPESKSF D+ LGPIPSR
Sbjct: 121 LHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSR 180

Query: 195 WRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGN 254
           W+GICQNQ D +FHCNRKLIGARYFNKGY + VGHLNSSF+SPRD +GHGSHTLSTA G+
Sbjct: 181 WKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGD 240

Query: 255 FVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLS 314
           FV G S+FG GNGTAKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+S
Sbjct: 241 FVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVIS 300

Query: 315 ISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDR 374
           +SLGG+P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +VSN+APW+ITVGAS+MDR
Sbjct: 301 VSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDR 360

Query: 375 KFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMK 434
           +F S +VLG+ K  +G+SLS  ALP   FYP+ ++ + K  NAS  +AQLCK G+LDP+K
Sbjct: 361 EFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIK 420

Query: 435 AKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGV 494
            KGKILVCLRG N RV+KG    L G  GM+L N+  +GN++LADPHVLPA+ +   D  
Sbjct: 421 TKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSF 480

Query: 495 AVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIA 554
           AV  YI+ TK P A+IT + T LG++PAP MA+FSS GP+ + P+ILKPD+TAPGVSVIA
Sbjct: 481 AVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIA 540

Query: 555 AYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTA 614
           AYT A  PTN+++D RR+LFN++SGTSMSCPH+SGIAGLL+T YP WSPAAIRSAIMTTA
Sbjct: 541 AYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTA 600

Query: 615 TTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQ 674
           T +D+   PI NA+  +ATPF++GAGHV PN A++PGLVYD+ +K+YL FLC+LGYN +Q
Sbjct: 601 TIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQ 660

Query: 675 ISQFADGPFNCAEP-ISLTNLNYPSITVPKLSRS-ITVTRRLKNVGSPGTYKAQIRKPLG 734
           IS F+   F C+ P ISL NLNYPSITVP L+ S +TV+R +KNVG P  Y  ++  P G
Sbjct: 661 ISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQG 720

Query: 735 ISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVVK 787
           + V VKP  LNFT+VGE+++FKV +   K NVAK YV+G+L+WSD KH VRSPIVVK
Sbjct: 721 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 771

BLAST of MC04g0288 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 2.3e-262
Identity = 459/781 (58.77%), Postives = 581/781 (74.39%), Query Frame = 0

Query: 14  TMKKTYSSSAFHH---LLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPA---D 73
           +M + YSS+ + +   L  L   LLL +L  SP  A+K+SY+VY+G+H+H  +  +   D
Sbjct: 2   SMTRRYSSTQYSNKMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLD 61

Query: 74  IVANSHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLN 133
            VA+SH  FL  F+  S E  ++ I YSY R+INGFAA+L+++ A ++AKH  VVSVF N
Sbjct: 62  GVAHSHRTFLASFV-GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPN 121

Query: 134 RGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGP 193
           +GRKLHTT SW FM L KNGV+   S+W KA +GEDTII NLDTG WPESKSFSD+  G 
Sbjct: 122 KGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGA 181

Query: 194 IPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVG-HLNSSFNSPRDNEGHGSHTLS 253
           +P+RW+G C         CNRKLIGARYFNKGY +  G   N+S+ + RD++GHGSHTLS
Sbjct: 182 VPARWKGRCHK----DVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLS 241

Query: 254 TAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDG 313
           TA GNFV GA+VFG+GNGTA GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DG
Sbjct: 242 TAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDG 301

Query: 314 VDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGA 373
           VDVLS S+GGD     +D +AIGSFHAVK+G+ V+CSAGNSGP +G+VSN+APW ITVGA
Sbjct: 302 VDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGA 361

Query: 374 SSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGT 433
           SSMDR+F + V L + +  +G SLS + LP    Y L SAAD  +AN +V +A LCK G+
Sbjct: 362 SSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGS 421

Query: 434 LDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHIN 493
           LDP K KGKILVCLRGDNARVDKG QA  AGAAGM+L N +ASGNEI++D HVLPAS I+
Sbjct: 422 LDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQID 481

Query: 494 FTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPG 553
           + DG  +F+Y++STK P  YI   T  L  +PAPFMA+FSS GPNTITP ILKPD+TAPG
Sbjct: 482 YKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPG 541

Query: 554 VSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSA 613
           V++IAA+TEA GPT+ + D RR  FN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAIRSA
Sbjct: 542 VNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSA 601

Query: 614 IMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALG 673
           IMTT+ T +N  +P+++ S+ +A PF+YG+GHV PN A  PGLVYD+   +YL FLCA+G
Sbjct: 602 IMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVG 661

Query: 674 YNQTQISQFADGP-FNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 733
           YN T +  FA+ P + C +  +L + NYPSITVP L+ SITVTR+LKNVG P TY A+ R
Sbjct: 662 YNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFR 721

Query: 734 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 787
           +PLG+ V V+PK+L F + GE + F++ ++         YV+G+L W+D  H+VRSPIVV
Sbjct: 722 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVV 775

BLAST of MC04g0288 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 710.3 bits (1832), Expect = 2.4e-203
Identity = 394/788 (50.00%), Postives = 516/788 (65.48%), Query Frame = 0

Query: 26  HLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPA---DIVANSHHEFLQPFLA 85
           HL+L   FL   + LL+  +  K+ Y+VYMGAHSHG    +   ++  +SH++ L     
Sbjct: 9   HLILSSFFLF--TFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFG 68

Query: 86  SSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEFMG 145
           S ++  ++ I+YSY R+INGFAA+LE++ A  +AK+ +VVSVFL++  KLHTTRSWEF+G
Sbjct: 69  SREK-AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLG 128

Query: 146 LEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWR-GICQNQSD 205
           L + G     S W+K RFGE+TIIGN+DTG WPES+SFSD   G +PS+WR G+CQ    
Sbjct: 129 LHRRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKL 188

Query: 206 PSF---HCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 265
           P      CNRKLIGARY+NK +E+  G L+   ++ RD  GHG+HTLSTAGGNFV GA V
Sbjct: 189 PGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARV 248

Query: 266 FGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD- 325
           F +GNGTAKGGSPRARVAAYKVCW       C+ AD+LAA D AI DGVDV+++S G   
Sbjct: 249 FAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSY 308

Query: 326 ---PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFP 385
                 +F D ++IG+FHA+   I+++ SAGN GPT G+V+N+APW  T+ AS++DR F 
Sbjct: 309 VVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFS 368

Query: 386 SRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKG 445
           S + + +N+ IEG SL    LP    + L  + D KLANA+  +AQLC+ GTLD  K  G
Sbjct: 369 SNLTI-NNQLIEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNG 428

Query: 446 KILVCLR-GDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVL------PASHINF 505
           KI++C R G    V +G +A+ AGA GMIL N   +G  + A+PHV       P    + 
Sbjct: 429 KIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSR 488

Query: 506 TDGVAVFAYINSTKSP-----TAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDV 565
             GV   A I     P     T  ++RA T  G +PAP MA+FSS GPN I P ILKPDV
Sbjct: 489 PHGVKTTA-IGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDV 548

Query: 566 TAPGVSVIAAYTEAEGPTNQEYDTRR-VLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPA 625
           TAPGV+++AAY+E    ++   D RR   FN + GTSMSCPH SGIAGLL+T +P WSPA
Sbjct: 549 TAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPA 608

Query: 626 AIRSAIMTTATTLDNNLEPILNA-SYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLL 685
           AI+SAIMTTATTLDN   PI +A   + A  F YG+GHV P+ AI+PGLVYD+ + +YL 
Sbjct: 609 AIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLN 668

Query: 686 FLCALGYNQTQISQF-ADGPFNCAEPISLTNLNYPSITVPKLS-RSITVTRRLKNVGSPG 745
           FLCA GY+Q  IS    +  F C+   S+ +LNYPSIT+P L  + +T+ R + NVG P 
Sbjct: 669 FLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPS 728

Query: 746 TYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHH 787
           TY    R P G S+ V P  L FT++GE ++FKV ++       + Y +GDL W+DGKH 
Sbjct: 729 TYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHI 787

BLAST of MC04g0288 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 1.9e-171
Identity = 344/774 (44.44%), Postives = 490/774 (63.31%), Query Frame = 0

Query: 21  SSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPF 80
           SS+F       L L L    +S +S+ + +Y+V+M       + P+    + H  +    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSS 61

Query: 81  LASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEF 140
           L S  +  E  +LY+Y   I+GF+  L  + A  L     V+SV      +LHTTR+  F
Sbjct: 62  LRSISDSAE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLF 121

Query: 141 MGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGICQNQS 200
           +GL+++    +  ++ +A    D ++G LDTG WPESKS+SD+  GPIPS W+G C+  +
Sbjct: 122 LGLDEH----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 181

Query: 201 D-PSFHCNRKLIGARYFNKGYESVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGAS 260
           +  +  CNRKLIGAR+F +GYES +G ++ S    SPRD++GHG+HT STA G+ V GAS
Sbjct: 182 NFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 241

Query: 261 VFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD 320
           + G  +GTA+G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG 
Sbjct: 242 LLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGG 301

Query: 321 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSRV 380
            +  + D VAIG+F A++ GI+V CSAGN+GP++ S+SN+APW  TVGA ++DR FP+  
Sbjct: 302 MSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALA 361

Query: 381 VLGSNKQIEGESL-SPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 440
           +LG+ K   G SL   EALP +   P   A +   +NA+     LC  GTL P K KGKI
Sbjct: 362 ILGNGKNFTGVSLFKGEALPDK-LLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKI 421

Query: 441 LVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 500
           ++C RG NARV KG+    AG  GMILAN+ A+G E++AD H+LPA+ +    G  +  Y
Sbjct: 422 VMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHY 481

Query: 501 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 560
           + +  +PTA I+   T +G++P+P +AAFSS GPN+ITP ILKPD+ APGV+++AA+T A
Sbjct: 482 VTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGA 541

Query: 561 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 620
            GPT    D+RRV FN +SGTSMSCPHVSG+A LL++++P WSPAAIRSA+MTTA     
Sbjct: 542 AGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYK 601

Query: 621 NLEPILN-ASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQF 680
           + +P+L+ A+   +TPF++GAGHV P  A +PGL+YD+  ++YL FLCAL Y   QI   
Sbjct: 602 DGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSV 661

Query: 681 ADGPFNC--AEPISLTNLNYPSITVPKLS-RSITVTRRLKNVGSPGTYKAQI-RKPLGIS 740
           +   + C  ++  S+ +LNYPS  V      +   TR + +VG  GTY  ++  +  G+ 
Sbjct: 662 SRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVK 721

Query: 741 VWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 786
           + V+P  LNF    E++S+ V   V+    + +  +G + WSDGKH V SP+ +
Sbjct: 722 ISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of MC04g0288 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 3.0e-169
Identity = 351/795 (44.15%), Postives = 468/795 (58.87%), Query Frame = 0

Query: 29  LLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPFLASSDEFV 88
           L PL  L+   LL+  +  K+ Y+VY G H   G +    +   HH +LQ  +  S+E  
Sbjct: 7   LFPLLFLIP--LLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQS-VKESEEDA 66

Query: 89  EDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK--LHTTRSWEFMGLEK- 148
              +LYSY   INGFAA L  D A+KL K   VVSVF +  RK   HTTRSWEF+GLE+ 
Sbjct: 67  RASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEE 126

Query: 149 --------------NGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSR 208
                         +   +  +  KKA+ G+  I+G LD+G WPESKSF+D  +GP+P  
Sbjct: 127 ETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKS 186

Query: 209 WRGICQ-NQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFN----SPRDNEGHGSHTLS 268
           W+GICQ   +  S HCNRK+IGARY+ KGYE   G  N++ N    SPRD +GHGSHT S
Sbjct: 187 WKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTAS 246

Query: 269 TAGGNFVAGASVF-GLGNGTAKGGSPRARVAAYKVCW-----PPAAGNECFDADILAAFD 328
           TA G  V GAS   G   G+A GG+P AR+A YK CW         GN C + D+LAA D
Sbjct: 247 TAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAID 306

Query: 329 LAIHDGVDVLSISLG-GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAP 388
            AI DGV V+SIS+G  +P P   D +A+G+ HAVK  IVV  SAGNSGP  G++SN+AP
Sbjct: 307 DAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAP 366

Query: 389 WEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEA 448
           W ITVGAS++DR F   +VLG+   I+ +S++  A     F PL  A++V +   +++E 
Sbjct: 367 WIITVGASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNET 426

Query: 449 QLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHV 508
             C   +L P    GK+++CLRG  +R+ KG +   AG AGMIL N  A+GNE+ +D H 
Sbjct: 427 SQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHF 486

Query: 509 LPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILK 568
           +P + +  T    +  YI + K+P A+I    T    + AP M  FSS GPN + P ILK
Sbjct: 487 VPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILK 546

Query: 569 PDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWS 628
           PD+TAPG+ ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LL+ ++P WS
Sbjct: 547 PDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 606

Query: 629 PAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYL 688
            AAIRSA+MTTA   ++  +PI + +   A PF  G+GH  P  A DPGLVYD   + YL
Sbjct: 607 SAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 689 LFLCALGYNQTQISQFADGPFNCAEPISL-TNLNYPSITVPKLSRSITVTRRLKNVG--- 748
           L+ C++  N T I    D  F C   I    N NYPSI VP L +++TV R + NVG   
Sbjct: 667 LYGCSV--NITNI----DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGN 726

Query: 749 SPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAK-----NYVYGDL 786
           S  TY   ++ P GISV   P  L+F R+G++Q FK+ +K  K  V        Y +G  
Sbjct: 727 STSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWF 786

BLAST of MC04g0288 vs. NCBI nr
Match: XP_022135592.1 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.3 [Momordica charantia])

HSP 1 Score: 1469 bits (3802), Expect = 0.0
Identity = 757/796 (95.10%), Postives = 760/796 (95.48%), Query Frame = 0

Query: 1   MRSKEEREREIAETMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSH 60
           MRSKEEREREIAETMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSH
Sbjct: 1   MRSKEEREREIAETMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSH 60

Query: 61  GGREPADIVANSHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRS 120
           GGREPADIVANSHHEFLQPFLAS DEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRS
Sbjct: 61  GGREPADIVANSHHEFLQPFLAS-DEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRS 120

Query: 121 VVSVFLNRGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSF 180
           VVSVFLNRGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTG +  S+  
Sbjct: 121 VVSVFLNRGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGIYLISRRX 180

Query: 181 SDD--------NLGPIPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFN 240
                      N   + S  R        P     RKLIGARYFNKGYESVVGHLNSSFN
Sbjct: 181 VXSSIVVLACLNYAKLISLDRXPXNLCKFPVIXTRRKLIGARYFNKGYESVVGHLNSSFN 240

Query: 241 SPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFD 300
           SPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFD
Sbjct: 241 SPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFD 300

Query: 301 ADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG 360
           ADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG
Sbjct: 301 ADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG 360

Query: 361 SVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLA 420
           SVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLA
Sbjct: 361 SVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLA 420

Query: 421 NASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNE 480
           NASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNE
Sbjct: 421 NASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNE 480

Query: 481 ILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNT 540
           ILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNT
Sbjct: 481 ILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNT 540

Query: 541 ITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLR 600
           ITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLR
Sbjct: 541 ITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLR 600

Query: 601 TLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYD 660
           TLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYD
Sbjct: 601 TLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYD 660

Query: 661 IEVKEYLLFLCALGYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLK 720
           IEVKEYLLFLCALGYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLK
Sbjct: 661 IEVKEYLLFLCALGYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLK 720

Query: 721 NVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIW 780
           NVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIW
Sbjct: 721 NVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIW 780

Query: 781 SDGKHHVRSPIVVKAV 788
           SDGKHHVRSPIVVKAV
Sbjct: 781 SDGKHHVRSPIVVKAV 795

BLAST of MC04g0288 vs. NCBI nr
Match: XP_038888965.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1358 bits (3516), Expect = 0.0
Identity = 680/773 (87.97%), Postives = 718/773 (92.88%), Query Frame = 0

Query: 18  TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 77
           T SS+ F    LLP FLLLSS LL+PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TISSATF----LLP-FLLLSSSLLTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHKFL 64

Query: 78  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 137
           +PFL   D+F EDVI YSYTR+INGFAAMLED+VA +LAKH  VVS+FLN+GRKLHTTRS
Sbjct: 65  RPFL-KRDDFTEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSIFLNKGRKLHTTRS 124

Query: 138 WEFMGLEKN-GVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGIC 197
           WEFMG+E N GVI SESIWKKARFGEDTIIGNLDTG WPESKSFSDD+LGPIP RWRGIC
Sbjct: 125 WEFMGMENNNGVITSESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPLRWRGIC 184

Query: 198 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 257
           QNQ+DPSFHCNRKLIGARYFNKGYESVVG LNSSFNSPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYESVVGPLNSSFNSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 258 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 317
           SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD AIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDSAIHDGVDVLSVSLGG 304

Query: 318 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 377
           DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGP AG+V+NIAPW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPAAGTVTNIAPWQITVGASTMDRKFPSL 364

Query: 378 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 437
           VVLG+ KQIEGESLS  ALPSR FYPL SAADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQNALPSRKFYPLVSAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 438 LVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 497
           LVCLRGDNARVDKGEQA+LAGA GMILAN+E SGNEILADPHVLPASHINFTDG AV  Y
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAVGMILANNEPSGNEILADPHVLPASHINFTDGTAVLTY 484

Query: 498 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 557
           INSTK P AYITRATTQLGIRPAPFMAAFSSVGPNTI PEILKPDVTAPG+SVIAAYTEA
Sbjct: 485 INSTKFPKAYITRATTQLGIRPAPFMAAFSSVGPNTIIPEILKPDVTAPGLSVIAAYTEA 544

Query: 558 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 617
           EGPTNQEYD RR+ FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA TLDN
Sbjct: 545 EGPTNQEYDNRRLPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTANTLDN 604

Query: 618 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 677
           N EP+LNASYS ATP NYGAGHVHPNGA DPGL+YDIEV EYLLFLCALGYNQ QISQF+
Sbjct: 605 NFEPLLNASYSVATPLNYGAGHVHPNGATDPGLIYDIEVNEYLLFLCALGYNQAQISQFS 664

Query: 678 DGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 737
            GPFNC+EPI+LTNLNYPSITVP+LSRSIT+TRRLKNVGSPGTYKAQIRKP GISVWVKP
Sbjct: 665 SGPFNCSEPINLTNLNYPSITVPELSRSITITRRLKNVGSPGTYKAQIRKPAGISVWVKP 724

Query: 738 KKLNFTRVGEEQSFKVFMKVEKQNVAK-NYVYGDLIWSDGKHHVRSPIVVKAV 788
           KKLNFTR+GEEQSFKV MKV++QN AK NYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTRLGEEQSFKVLMKVKEQNAAKKNYVYGDLIWSDGKHHVRSPIVVKVV 771

BLAST of MC04g0288 vs. NCBI nr
Match: TYK03381.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1343 bits (3476), Expect = 0.0
Identity = 669/773 (86.55%), Postives = 716/773 (92.63%), Query Frame = 0

Query: 18  TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 77
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 78  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 137
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 138 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGIC 197
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTG W ESKSFSDD +GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 198 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 257
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 258 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 317
           SVFGLG GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 318 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 377
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 378 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 437
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 438 LVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 497
           LVCLRGDNARVDKGEQA+LAGAAGMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 498 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 557
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 558 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 617
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 618 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 677
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 678 DGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 737
           DGPFNC+EPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 738 KKLNFTRVGEEQSFKVFMKVEKQNVAK-NYVYGDLIWSDGKHHVRSPIVVKAV 788
           KKLNFT++GEE SFKVFMKV+   VAK NYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MC04g0288 vs. NCBI nr
Match: XP_008462019.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])

HSP 1 Score: 1343 bits (3476), Expect = 0.0
Identity = 669/773 (86.55%), Postives = 716/773 (92.63%), Query Frame = 0

Query: 18  TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 77
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 78  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 137
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 138 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGIC 197
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTG W ESKSFSDD +GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 198 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 257
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 258 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 317
           SVFGLG GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 318 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 377
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 378 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 437
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 438 LVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 497
           LVCLRGDNARVDKGEQA+LAGAAGMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 498 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 557
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 558 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 617
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 618 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 677
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 678 DGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 737
           DGPFNC+EPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 738 KKLNFTRVGEEQSFKVFMKVEKQNVAK-NYVYGDLIWSDGKHHVRSPIVVKAV 788
           KKLNFT++GEE SFKVFMKV+   VAK NYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MC04g0288 vs. NCBI nr
Match: XP_022968976.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1338 bits (3463), Expect = 0.0
Identity = 672/781 (86.04%), Postives = 719/781 (92.06%), Query Frame = 0

Query: 13  ETMKK-TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVAN 72
           ETMK+ TY S+ F       L LLLSSLLL+PT+AIKRSYVVYMGAHSHGGR+PADIVA+
Sbjct: 21  ETMKQPTYPSTTF-------LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVAD 80

Query: 73  SHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK 132
           SH E L+P+L S +EF EDVI YSYTR+INGFAAMLED+VA +LAKH  VVSVF NRGR+
Sbjct: 81  SHRELLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRR 140

Query: 133 LHTTRSWEFMGLEKN-GVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSD-DNLGPIP 192
           LHTTRSWEFM LE N GVI SESIWKKARFGEDTIIGNLDTG WPESKSFSD D+LGPIP
Sbjct: 141 LHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIP 200

Query: 193 SRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAG 252
            RWRGICQNQ+DPSFHCNRKLIGARYFNKGY+SVVGHLNSSFNSPRDNEGHGSHTLSTAG
Sbjct: 201 QRWRGICQNQNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAG 260

Query: 253 GNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDV 312
           GNFVAGASVFGLG G AKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDV
Sbjct: 261 GNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDV 320

Query: 313 LSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSM 372
           LS+SLGGDPNPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSV+NIAPW+ITVGAS+M
Sbjct: 321 LSVSLGGDPNPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTM 380

Query: 373 DRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDP 432
           DRKFPSRVVLG+ KQIEGESL+PE LPSR FYPL SAADV+LANAS HEAQLCKAGTLDP
Sbjct: 381 DRKFPSRVVLGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDP 440

Query: 433 MKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTD 492
            KAKGKILVCLRGDNARVDKGEQAMLAGA GMILAN+E SGNEILADPHVLPASHINFTD
Sbjct: 441 KKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTD 500

Query: 493 GVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSV 552
           GVAVFAYINSTK P AYIT ATTQLG RPAPFMAAFSSVGP+TITPEILKPDVTAPG+SV
Sbjct: 501 GVAVFAYINSTKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSV 560

Query: 553 IAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMT 612
           IAAYTEAEGPTNQEYD RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMT
Sbjct: 561 IAAYTEAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMT 620

Query: 613 TATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQ 672
           TA+TLDNN +P+LNASYS ATPFNYGAGH+HPN A DPGLVYDI + EYL FLCALGYN+
Sbjct: 621 TASTLDNNFKPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNK 680

Query: 673 TQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLG 732
            Q+SQF++GPFNC+EPISLTNLNYPSITVP LSRSIT+TRRLKNVGSPGTYKA+IR+P G
Sbjct: 681 AQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAG 740

Query: 733 ISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVA--KNYVYGDLIWSDGKHHVRSPIVVKA 788
           ISV VKPKKL+FTR+GEE SFKV MKV++ NV   KNYVYGDLIWSDGKHHVRSPIVV A
Sbjct: 741 ISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMA 794

BLAST of MC04g0288 vs. ExPASy TrEMBL
Match: A0A6J1C351 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111007513 PE=3 SV=1)

HSP 1 Score: 1469 bits (3802), Expect = 0.0
Identity = 757/796 (95.10%), Postives = 760/796 (95.48%), Query Frame = 0

Query: 1   MRSKEEREREIAETMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSH 60
           MRSKEEREREIAETMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSH
Sbjct: 1   MRSKEEREREIAETMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSH 60

Query: 61  GGREPADIVANSHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRS 120
           GGREPADIVANSHHEFLQPFLAS DEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRS
Sbjct: 61  GGREPADIVANSHHEFLQPFLAS-DEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRS 120

Query: 121 VVSVFLNRGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSF 180
           VVSVFLNRGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTG +  S+  
Sbjct: 121 VVSVFLNRGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGIYLISRRX 180

Query: 181 SDD--------NLGPIPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFN 240
                      N   + S  R        P     RKLIGARYFNKGYESVVGHLNSSFN
Sbjct: 181 VXSSIVVLACLNYAKLISLDRXPXNLCKFPVIXTRRKLIGARYFNKGYESVVGHLNSSFN 240

Query: 241 SPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFD 300
           SPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFD
Sbjct: 241 SPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFD 300

Query: 301 ADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG 360
           ADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG
Sbjct: 301 ADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG 360

Query: 361 SVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLA 420
           SVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLA
Sbjct: 361 SVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLA 420

Query: 421 NASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNE 480
           NASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNE
Sbjct: 421 NASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNE 480

Query: 481 ILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNT 540
           ILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNT
Sbjct: 481 ILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNT 540

Query: 541 ITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLR 600
           ITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLR
Sbjct: 541 ITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLR 600

Query: 601 TLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYD 660
           TLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYD
Sbjct: 601 TLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYD 660

Query: 661 IEVKEYLLFLCALGYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLK 720
           IEVKEYLLFLCALGYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLK
Sbjct: 661 IEVKEYLLFLCALGYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLK 720

Query: 721 NVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIW 780
           NVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIW
Sbjct: 721 NVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIW 780

Query: 781 SDGKHHVRSPIVVKAV 788
           SDGKHHVRSPIVVKAV
Sbjct: 781 SDGKHHVRSPIVVKAV 795

BLAST of MC04g0288 vs. ExPASy TrEMBL
Match: A0A5D3BUG6 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84663G00160 PE=3 SV=1)

HSP 1 Score: 1343 bits (3476), Expect = 0.0
Identity = 669/773 (86.55%), Postives = 716/773 (92.63%), Query Frame = 0

Query: 18  TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 77
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 78  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 137
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 138 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGIC 197
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTG W ESKSFSDD +GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 198 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 257
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 258 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 317
           SVFGLG GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 318 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 377
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 378 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 437
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 438 LVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 497
           LVCLRGDNARVDKGEQA+LAGAAGMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 498 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 557
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 558 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 617
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 618 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 677
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 678 DGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 737
           DGPFNC+EPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 738 KKLNFTRVGEEQSFKVFMKVEKQNVAK-NYVYGDLIWSDGKHHVRSPIVVKAV 788
           KKLNFT++GEE SFKVFMKV+   VAK NYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MC04g0288 vs. ExPASy TrEMBL
Match: A0A1S3CG03 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500491 PE=3 SV=1)

HSP 1 Score: 1343 bits (3476), Expect = 0.0
Identity = 669/773 (86.55%), Postives = 716/773 (92.63%), Query Frame = 0

Query: 18  TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 77
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 78  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 137
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 138 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGIC 197
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTG W ESKSFSDD +GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 198 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 257
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 258 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 317
           SVFGLG GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 318 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 377
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 378 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 437
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 438 LVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 497
           LVCLRGDNARVDKGEQA+LAGAAGMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 498 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 557
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 558 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 617
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 618 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 677
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 678 DGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 737
           DGPFNC+EPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 738 KKLNFTRVGEEQSFKVFMKVEKQNVAK-NYVYGDLIWSDGKHHVRSPIVVKAV 788
           KKLNFT++GEE SFKVFMKV+   VAK NYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MC04g0288 vs. ExPASy TrEMBL
Match: A0A6J1HWD2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468122 PE=3 SV=1)

HSP 1 Score: 1338 bits (3463), Expect = 0.0
Identity = 672/781 (86.04%), Postives = 719/781 (92.06%), Query Frame = 0

Query: 13  ETMKK-TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVAN 72
           ETMK+ TY S+ F       L LLLSSLLL+PT+AIKRSYVVYMGAHSHGGR+PADIVA+
Sbjct: 21  ETMKQPTYPSTTF-------LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVAD 80

Query: 73  SHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK 132
           SH E L+P+L S +EF EDVI YSYTR+INGFAAMLED+VA +LAKH  VVSVF NRGR+
Sbjct: 81  SHRELLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRR 140

Query: 133 LHTTRSWEFMGLEKN-GVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSD-DNLGPIP 192
           LHTTRSWEFM LE N GVI SESIWKKARFGEDTIIGNLDTG WPESKSFSD D+LGPIP
Sbjct: 141 LHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIP 200

Query: 193 SRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAG 252
            RWRGICQNQ+DPSFHCNRKLIGARYFNKGY+SVVGHLNSSFNSPRDNEGHGSHTLSTAG
Sbjct: 201 QRWRGICQNQNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAG 260

Query: 253 GNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDV 312
           GNFVAGASVFGLG G AKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDV
Sbjct: 261 GNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDV 320

Query: 313 LSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSM 372
           LS+SLGGDPNPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSV+NIAPW+ITVGAS+M
Sbjct: 321 LSVSLGGDPNPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTM 380

Query: 373 DRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDP 432
           DRKFPSRVVLG+ KQIEGESL+PE LPSR FYPL SAADV+LANAS HEAQLCKAGTLDP
Sbjct: 381 DRKFPSRVVLGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDP 440

Query: 433 MKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTD 492
            KAKGKILVCLRGDNARVDKGEQAMLAGA GMILAN+E SGNEILADPHVLPASHINFTD
Sbjct: 441 KKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTD 500

Query: 493 GVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSV 552
           GVAVFAYINSTK P AYIT ATTQLG RPAPFMAAFSSVGP+TITPEILKPDVTAPG+SV
Sbjct: 501 GVAVFAYINSTKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSV 560

Query: 553 IAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMT 612
           IAAYTEAEGPTNQEYD RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMT
Sbjct: 561 IAAYTEAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMT 620

Query: 613 TATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQ 672
           TA+TLDNN +P+LNASYS ATPFNYGAGH+HPN A DPGLVYDI + EYL FLCALGYN+
Sbjct: 621 TASTLDNNFKPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNK 680

Query: 673 TQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLG 732
            Q+SQF++GPFNC+EPISLTNLNYPSITVP LSRSIT+TRRLKNVGSPGTYKA+IR+P G
Sbjct: 681 AQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAG 740

Query: 733 ISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVA--KNYVYGDLIWSDGKHHVRSPIVVKA 788
           ISV VKPKKL+FTR+GEE SFKV MKV++ NV   KNYVYGDLIWSDGKHHVRSPIVV A
Sbjct: 741 ISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMA 794

BLAST of MC04g0288 vs. ExPASy TrEMBL
Match: A0A6J1GL32 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111455314 PE=3 SV=1)

HSP 1 Score: 1326 bits (3432), Expect = 0.0
Identity = 662/761 (86.99%), Postives = 708/761 (93.04%), Query Frame = 0

Query: 32  LFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPFLASSDEFVEDV 91
           L LLLSSLLL+PT+AIKRSYVVYMGAHSHGGR+PA+IVA+SH + L+P+L S +EF EDV
Sbjct: 12  LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVADSHRDLLRPYLESGNEFAEDV 71

Query: 92  ILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEFMGLEKN-GVII 151
           I YSYTR+INGFAAMLED VA +LA+H  VVSVF NRGR+LHTTRSWEFM LE N GVI 
Sbjct: 72  IFYSYTRHINGFAAMLEDQVAARLAQHPEVVSVFPNRGRRLHTTRSWEFMELENNHGVIS 131

Query: 152 SESIWKKARFGEDTIIGNLDTGAWPESKSFSD-DNLGPIPSRWRGICQNQSDPSFHCNRK 211
           SESIWKKARFGEDTIIGNLDTG WPESKSFSD D+LGPIP RWRGICQNQ+DPSFHCNRK
Sbjct: 132 SESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQNQNDPSFHCNRK 191

Query: 212 LIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGG 271
           LIGARYFNKGY+SVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLG G AKGG
Sbjct: 192 LIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGG 251

Query: 272 SPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIG 331
           SPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVLS+SLGGDPNPLFNDSVAIG
Sbjct: 252 SPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIG 311

Query: 332 SFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGES 391
           SFHAVKHGIVVICSAGNSGPTAGSV+NIAPW+ITVGAS+MDRKFPSRVVLG+ KQIEGES
Sbjct: 312 SFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIEGES 371

Query: 392 LSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDK 451
           L+PE LPSR FYPL SAA+V+LANAS HEAQLCKAGTLDP KAKGKILVCLRGDNARVDK
Sbjct: 372 LTPEGLPSRKFYPLMSAANVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDK 431

Query: 452 GEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITR 511
           GEQAMLAGA GMILAN+E SGNEILADPHVLPASHINFTDGVAVFAY+NSTK P AYIT 
Sbjct: 432 GEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYVNSTKFPQAYITG 491

Query: 512 ATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRV 571
           ATTQLGIRPAPFMAAFSSVGP+TITPEILKPDVTAPG+SVIAAYT AEGPTNQEYD RRV
Sbjct: 492 ATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRV 551

Query: 572 LFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEA 631
            FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAI+TTA+TLDNN +P+LNASYS A
Sbjct: 552 AFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIVTTASTLDNNFKPLLNASYSVA 611

Query: 632 TPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFADGPFNCAEPISLT 691
           TPFNYGAGH+HPN A DPGLVYDI V EYL FLCALGYN+ Q+SQF++GPFNC+EPISLT
Sbjct: 612 TPFNYGAGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLT 671

Query: 692 NLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQS 751
           NLNYPSITVP LSRSIT+TRRLKNVGSPGTYKA+IRKP GISV VKPKKL+FTR+GEE S
Sbjct: 672 NLNYPSITVPNLSRSITITRRLKNVGSPGTYKAKIRKPAGISVSVKPKKLSFTRLGEELS 731

Query: 752 FKVFMKVEKQNVA--KNYVYGDLIWSDGKHHVRSPIVVKAV 788
           FKV MKV++ NV   KNYVYGDLIWSDGKHHVRSPIVV AV
Sbjct: 732 FKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 772

BLAST of MC04g0288 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 986.9 bits (2550), Expect = 9.6e-288
Identity = 492/777 (63.32%), Postives = 599/777 (77.09%), Query Frame = 0

Query: 15  MKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGG---REPADIVAN 74
           MK T++   F  LLLL L  + S  +L+  S    SYVVY GAHSH G    +  D V  
Sbjct: 1   MKLTHN---FSFLLLLLLVHMSSKHILA--SKDSSSYVVYFGAHSHVGEITEDAMDRVKE 60

Query: 75  SHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK 134
           +H++FL  F   S E   D I YSYT++INGFAA L+ D+A +++KH  VVSVF N+  K
Sbjct: 61  THYDFLGSF-TGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALK 120

Query: 135 LHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSR 194
           LHTTRSW+F+GLE N  + S SIW+KARFGEDTII NLDTG WPESKSF D+ LGPIPSR
Sbjct: 121 LHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSR 180

Query: 195 WRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGN 254
           W+GICQNQ D +FHCNRKLIGARYFNKGY + VGHLNSSF+SPRD +GHGSHTLSTA G+
Sbjct: 181 WKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGD 240

Query: 255 FVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLS 314
           FV G S+FG GNGTAKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+S
Sbjct: 241 FVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVIS 300

Query: 315 ISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDR 374
           +SLGG+P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +VSN+APW+ITVGAS+MDR
Sbjct: 301 VSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDR 360

Query: 375 KFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMK 434
           +F S +VLG+ K  +G+SLS  ALP   FYP+ ++ + K  NAS  +AQLCK G+LDP+K
Sbjct: 361 EFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIK 420

Query: 435 AKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGV 494
            KGKILVCLRG N RV+KG    L G  GM+L N+  +GN++LADPHVLPA+ +   D  
Sbjct: 421 TKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSF 480

Query: 495 AVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIA 554
           AV  YI+ TK P A+IT + T LG++PAP MA+FSS GP+ + P+ILKPD+TAPGVSVIA
Sbjct: 481 AVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIA 540

Query: 555 AYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTA 614
           AYT A  PTN+++D RR+LFN++SGTSMSCPH+SGIAGLL+T YP WSPAAIRSAIMTTA
Sbjct: 541 AYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTA 600

Query: 615 TTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQ 674
           T +D+   PI NA+  +ATPF++GAGHV PN A++PGLVYD+ +K+YL FLC+LGYN +Q
Sbjct: 601 TIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQ 660

Query: 675 ISQFADGPFNCAEP-ISLTNLNYPSITVPKLSRS-ITVTRRLKNVGSPGTYKAQIRKPLG 734
           IS F+   F C+ P ISL NLNYPSITVP L+ S +TV+R +KNVG P  Y  ++  P G
Sbjct: 661 ISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQG 720

Query: 735 ISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVVK 787
           + V VKP  LNFT+VGE+++FKV +   K NVAK YV+G+L+WSD KH VRSPIVVK
Sbjct: 721 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 771

BLAST of MC04g0288 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 906.4 bits (2341), Expect = 1.6e-263
Identity = 459/781 (58.77%), Postives = 581/781 (74.39%), Query Frame = 0

Query: 14  TMKKTYSSSAFHH---LLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPA---D 73
           +M + YSS+ + +   L  L   LLL +L  SP  A+K+SY+VY+G+H+H  +  +   D
Sbjct: 2   SMTRRYSSTQYSNKMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLD 61

Query: 74  IVANSHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLN 133
            VA+SH  FL  F+  S E  ++ I YSY R+INGFAA+L+++ A ++AKH  VVSVF N
Sbjct: 62  GVAHSHRTFLASFV-GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPN 121

Query: 134 RGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGP 193
           +GRKLHTT SW FM L KNGV+   S+W KA +GEDTII NLDTG WPESKSFSD+  G 
Sbjct: 122 KGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGA 181

Query: 194 IPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVG-HLNSSFNSPRDNEGHGSHTLS 253
           +P+RW+G C         CNRKLIGARYFNKGY +  G   N+S+ + RD++GHGSHTLS
Sbjct: 182 VPARWKGRCHK----DVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLS 241

Query: 254 TAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDG 313
           TA GNFV GA+VFG+GNGTA GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DG
Sbjct: 242 TAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDG 301

Query: 314 VDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGA 373
           VDVLS S+GGD     +D +AIGSFHAVK+G+ V+CSAGNSGP +G+VSN+APW ITVGA
Sbjct: 302 VDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGA 361

Query: 374 SSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGT 433
           SSMDR+F + V L + +  +G SLS + LP    Y L SAAD  +AN +V +A LCK G+
Sbjct: 362 SSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGS 421

Query: 434 LDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHIN 493
           LDP K KGKILVCLRGDNARVDKG QA  AGAAGM+L N +ASGNEI++D HVLPAS I+
Sbjct: 422 LDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQID 481

Query: 494 FTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPG 553
           + DG  +F+Y++STK P  YI   T  L  +PAPFMA+FSS GPNTITP ILKPD+TAPG
Sbjct: 482 YKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPG 541

Query: 554 VSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSA 613
           V++IAA+TEA GPT+ + D RR  FN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAIRSA
Sbjct: 542 VNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSA 601

Query: 614 IMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALG 673
           IMTT+ T +N  +P+++ S+ +A PF+YG+GHV PN A  PGLVYD+   +YL FLCA+G
Sbjct: 602 IMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVG 661

Query: 674 YNQTQISQFADGP-FNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 733
           YN T +  FA+ P + C +  +L + NYPSITVP L+ SITVTR+LKNVG P TY A+ R
Sbjct: 662 YNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFR 721

Query: 734 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 787
           +PLG+ V V+PK+L F + GE + F++ ++         YV+G+L W+D  H+VRSPIVV
Sbjct: 722 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVV 775

BLAST of MC04g0288 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 604.4 bits (1557), Expect = 1.3e-172
Identity = 344/774 (44.44%), Postives = 490/774 (63.31%), Query Frame = 0

Query: 21  SSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPF 80
           SS+F       L L L    +S +S+ + +Y+V+M       + P+    + H  +    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSS 61

Query: 81  LASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEF 140
           L S  +  E  +LY+Y   I+GF+  L  + A  L     V+SV      +LHTTR+  F
Sbjct: 62  LRSISDSAE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLF 121

Query: 141 MGLEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGICQNQS 200
           +GL+++    +  ++ +A    D ++G LDTG WPESKS+SD+  GPIPS W+G C+  +
Sbjct: 122 LGLDEH----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 181

Query: 201 D-PSFHCNRKLIGARYFNKGYESVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGAS 260
           +  +  CNRKLIGAR+F +GYES +G ++ S    SPRD++GHG+HT STA G+ V GAS
Sbjct: 182 NFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 241

Query: 261 VFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD 320
           + G  +GTA+G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG 
Sbjct: 242 LLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGG 301

Query: 321 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSRV 380
            +  + D VAIG+F A++ GI+V CSAGN+GP++ S+SN+APW  TVGA ++DR FP+  
Sbjct: 302 MSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALA 361

Query: 381 VLGSNKQIEGESL-SPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 440
           +LG+ K   G SL   EALP +   P   A +   +NA+     LC  GTL P K KGKI
Sbjct: 362 ILGNGKNFTGVSLFKGEALPDK-LLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKI 421

Query: 441 LVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 500
           ++C RG NARV KG+    AG  GMILAN+ A+G E++AD H+LPA+ +    G  +  Y
Sbjct: 422 VMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHY 481

Query: 501 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 560
           + +  +PTA I+   T +G++P+P +AAFSS GPN+ITP ILKPD+ APGV+++AA+T A
Sbjct: 482 VTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGA 541

Query: 561 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 620
            GPT    D+RRV FN +SGTSMSCPHVSG+A LL++++P WSPAAIRSA+MTTA     
Sbjct: 542 AGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYK 601

Query: 621 NLEPILN-ASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQF 680
           + +P+L+ A+   +TPF++GAGHV P  A +PGL+YD+  ++YL FLCAL Y   QI   
Sbjct: 602 DGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSV 661

Query: 681 ADGPFNC--AEPISLTNLNYPSITVPKLS-RSITVTRRLKNVGSPGTYKAQI-RKPLGIS 740
           +   + C  ++  S+ +LNYPS  V      +   TR + +VG  GTY  ++  +  G+ 
Sbjct: 662 SRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVK 721

Query: 741 VWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 786
           + V+P  LNF    E++S+ V   V+    + +  +G + WSDGKH V SP+ +
Sbjct: 722 ISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of MC04g0288 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 597.0 bits (1538), Expect = 2.1e-170
Identity = 351/795 (44.15%), Postives = 468/795 (58.87%), Query Frame = 0

Query: 29  LLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPFLASSDEFV 88
           L PL  L+   LL+  +  K+ Y+VY G H   G +    +   HH +LQ  +  S+E  
Sbjct: 7   LFPLLFLIP--LLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQS-VKESEEDA 66

Query: 89  EDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK--LHTTRSWEFMGLEK- 148
              +LYSY   INGFAA L  D A+KL K   VVSVF +  RK   HTTRSWEF+GLE+ 
Sbjct: 67  RASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEE 126

Query: 149 --------------NGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSR 208
                         +   +  +  KKA+ G+  I+G LD+G WPESKSF+D  +GP+P  
Sbjct: 127 ETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKS 186

Query: 209 WRGICQ-NQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFN----SPRDNEGHGSHTLS 268
           W+GICQ   +  S HCNRK+IGARY+ KGYE   G  N++ N    SPRD +GHGSHT S
Sbjct: 187 WKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTAS 246

Query: 269 TAGGNFVAGASVF-GLGNGTAKGGSPRARVAAYKVCW-----PPAAGNECFDADILAAFD 328
           TA G  V GAS   G   G+A GG+P AR+A YK CW         GN C + D+LAA D
Sbjct: 247 TAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAID 306

Query: 329 LAIHDGVDVLSISLG-GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAP 388
            AI DGV V+SIS+G  +P P   D +A+G+ HAVK  IVV  SAGNSGP  G++SN+AP
Sbjct: 307 DAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAP 366

Query: 389 WEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEA 448
           W ITVGAS++DR F   +VLG+   I+ +S++  A     F PL  A++V +   +++E 
Sbjct: 367 WIITVGASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNET 426

Query: 449 QLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHV 508
             C   +L P    GK+++CLRG  +R+ KG +   AG AGMIL N  A+GNE+ +D H 
Sbjct: 427 SQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHF 486

Query: 509 LPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILK 568
           +P + +  T    +  YI + K+P A+I    T    + AP M  FSS GPN + P ILK
Sbjct: 487 VPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILK 546

Query: 569 PDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWS 628
           PD+TAPG+ ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LL+ ++P WS
Sbjct: 547 PDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 606

Query: 629 PAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYL 688
            AAIRSA+MTTA   ++  +PI + +   A PF  G+GH  P  A DPGLVYD   + YL
Sbjct: 607 SAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 689 LFLCALGYNQTQISQFADGPFNCAEPISL-TNLNYPSITVPKLSRSITVTRRLKNVG--- 748
           L+ C++  N T I    D  F C   I    N NYPSI VP L +++TV R + NVG   
Sbjct: 667 LYGCSV--NITNI----DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGN 726

Query: 749 SPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAK-----NYVYGDL 786
           S  TY   ++ P GISV   P  L+F R+G++Q FK+ +K  K  V        Y +G  
Sbjct: 727 STSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWF 786

BLAST of MC04g0288 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 588.6 bits (1516), Expect = 7.6e-168
Identity = 330/723 (45.64%), Postives = 458/723 (63.35%), Query Frame = 0

Query: 84  SDEFVEDV-ILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEFMG 143
           S EF E+  I++ Y    +GF+A++  D A  L  H +V++VF +R R+LHTTRS +F+G
Sbjct: 49  STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108

Query: 144 LEKNGVIISESIWKKARFGEDTIIGNLDTGAWPESKSFSDDNLGPIPSRWRGICQNQSDP 203
           L+       + +W ++ +G D IIG  DTG WPE +SFSD NLGPIP RWRG+C++ +  
Sbjct: 109 LQN-----QKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARF 168

Query: 204 S-FHCNRKLIGARYFNKGYE-SVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGASV 263
           S  +CNRK+IGAR+F KG + +V+G +N +  F SPRD +GHG+HT STA G     AS+
Sbjct: 169 SPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASM 228

Query: 264 FGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD- 323
            G  +G AKG +P+AR+AAYKVCW  +    C D+DILAAFD A+ DGVDV+SIS+GG  
Sbjct: 229 SGYASGVAKGVAPKARIAAYKVCWKDSG---CLDSDILAAFDAAVRDGVDVISISIGGGD 288

Query: 324 --PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPS 383
              +P + D +AIGS+ A   GI V  SAGN GP   SV+N+APW  TVGAS++DR FP+
Sbjct: 289 GITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPA 348

Query: 384 RVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGK 443
             +LG   ++ G SL      +   +P+     V    + +  A LC   TLDP + +GK
Sbjct: 349 DAILGDGHRLRGVSLYAGVPLNGRMFPV-----VYPGKSGMSSASLCMENTLDPKQVRGK 408

Query: 444 ILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHINFTDGVAVFA 503
           I++C RG + RV KG     AG  GMILAN  ++G  ++ D H++PA  +   +G  + A
Sbjct: 409 IVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKA 468

Query: 504 YINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTE 563
           Y +S  +P A I    T +GI+PAP +A+FS  GPN ++PEILKPD+ APGV+++AA+T+
Sbjct: 469 YASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTD 528

Query: 564 AEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLD 623
           A GPT    D R+  FN +SGTSM+CPHVSG A LL++ +P WSPA IRSA+MTT   +D
Sbjct: 529 AVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 588

Query: 624 NNLEPILNASYSE-ATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQ 683
           N+   +++ S  + ATP++YG+GH++   A++PGLVYDI   +Y+ FLC++GY    I  
Sbjct: 589 NSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQV 648

Query: 684 FADGPFNC--AEPISLTNLNYPSITV--PKLSRSI---TVTRRLKNVG-SPGTYKAQIRK 743
               P  C      S  NLNYPSIT   P   R +   TV R   NVG +   Y+A+I  
Sbjct: 649 ITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIES 708

Query: 744 PLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVA---KNYVYGDLIWSD-GKHHVRSP 786
           P G++V VKP +L FT   + +S+ V + V  +NV       V+G + W D GKH VRSP
Sbjct: 709 PRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSP 758