MC04g0215 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0215
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionubiquitin fusion degradation protein 1
LocationMC04: 1683878 .. 1687740 (-)
RNA-Seq ExpressionMC04g0215
SyntenyMC04g0215
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATCACTGAATAAGAGTAGCGACAAAATTTCCTACTTTTGAGAGTGGATACGCTGCGTTTTGGATTTGGGCCTTGGTGTGGGAAATGGGCCGGGCCATCGAGTGGAAAGGAAAGGCCCAGAAGGTTACAGAACCAAACCCAATTGGTGCCCATCTACCGTCCATCTTTGACCGTCAAAATCCGATTTGGTAATTCTGCCGGAAGGCGGAAGCTTTCGTCTCCGTTTCCTCACCAGCAAATTCATAAATACAGTACAGCCAACTCCGCATTCTTCAATTTGTTTCGCAATTTCTGATCGGAGACAAATCTTACACCGGGAGAGATTCAGGTAAAAAGAGGAGAGGGCACAGATTTTGGAAGAGATGGATTTCGAGCTTAGAAGGGCGAGAGAGAAGCTCGAAAAGGAGCAGAAGGAGAGGAAGGAGATGGCTCGGCGGAGAGTCGAGAGAGAGCGCAAAGCTAAAGAAGAGGCGCGGAAGCAACGAGAATCCATTGAAGCGGCTCAACTTTCTAGACGACTTGATGCTGCCGAGGCCCGAATTAAGGTGCGCCTACTACTGATTTCTTTTTCAGATTGAGCTAATGGTGGAATTCACCTTAGATATGGCTATCTTAATTAAATGTTTAAGTTTATGAATGGTGATCGCATCCCAATTGATCTTTGTGTTCTTTCTTTTTGTTTCTTGGCTTGCTGAGGAAGTTATTTCATTTCTGAATCGAGACTGCGAAACTTTGTGCAACTTCTTTAATACGGTCTTAACTTTTAAGCATACTCTATAGTATATCCTTAGATAGTTAGGCATTCTTTGGGTCTTTTGAAATACACTTGCAATCACCCGATGGACGCAGTGGCTGAAAGTATTTCATGGTATTCTGAAATTTCTGGTTCCTGGATTTTTCCGGGTTGCCGTTTCGAGGAAATTGAAACATTTAGAAACACGCCTTCCTTAACGCACATAACTTAGAAAACTTTCTATGATTGGGCACTGGGAAAATGATCTCCTTAAATGTTGTGCTTTTGTTGCATCTTTTGTGCTTAACTTGCTTGGCATAAGGCATCCTGGTTAATTTTGTGAATGGGTTTTTAAAATTAAGGTGTGATGTTGACACTAATCTGCTAGGAAAGTGGGCAAGCTGACTCCCGAAGCTTTGTCATACGAGAGAGAGCTTTCTATGACTCTATGGGAAGGAAAGGATTATTTATTCAAACAGGACTTTGGAGCCTGTTTTTGGAGGCTGTATTTAGAACTTTTATGTCTTCATAAGTTGAGATGGGCTGTATTTAAACAAATATTTCTAGCTCAAGAACTAATTTTACCTAATGATCTCAGAATCGATTTTCATAGGAAAAGTAAAAATTGGAAGATAGGATGTTTGGCAGATTGTTCTGTAGAACAATTTTTAAAGATTATTTTTAAGAACTATTTATTGTTGTAAGTTGTGCAGAAGTCAAAGAGTCCCACAAAAGTAAATATTTTAGTTTGGCTTGTCTTCATGGAAATTCAAAATTTTATTGACAGAGTGCAGAAAAGATGTCCCTTTTGGACGTTTAAATATCTTGATGAAAAGATACATATTGAATGTTTTCTGAAACTTAGTGAAAATTGAAAATTCCGTAGCATCCACCTAGGTGATGAACTGGTATAGAGCTGTAGTTATGTTCACTATACGTTCTTTTTGGTTGGACTTGTACATTTTTCCATATCCTCGCTACCTGGCATCGTTAATTGGTGTACATGTTGAAAATTATGAATTTTGGAATTTGCAATTTTCCCTTCGCAAGTGGCATTGTGCCGATCATCCACTTTTTACCATGCATTTTTCCCTCTCTTTTTGACGAAGGCTTTAAACATTCCAGGCTGAGCAACTGATGGAAGAAACTTTGCTTGCTGGAAGAGGAATCACTTTTTACCGTCTCTTAGAAGCCATACCTTATCAGGGTAATGGGGACAAGATCAAGCTGCCGCCTTCTGGTTTCACAGAATTGTCCGATCAAAGTGCTTTTGATAAGGGACCTGTGTACTTCCAACTGTCAGTAGTTCATCAAGAAGGTACTTCCAACACAAACATTATGGAGGAAAAGACTCACAGAATCACCCATTCAGGTGTTTTGGAGTTCACTGCAGCTGAAGGTTTTGTTGAGCTACCTCCTCATGTTTGGCGAAATTTATTTCTGGACGACTCTTCAAAGAAGCCACTAATTGAAGTACGTTATGTGTGGCTACCCAAAGGAACCTATGCAAAGCTTCAACCAGAAGGCATAGGCTTTTTGGACTTGCCTAATCATAAAGCTATCCTTGAGACAAGCCTTCGTCAGCATGCCACTCTTTCTCAAGGTGACATACTGACTGTCGTCTACAGTGAGCTTACATATAAGTTAAATGTTCTTGAGTTGAAACCATCTTCTAGTATATCTGTTCTAGAAACGGATATTGAGGTTGATATAGTTGGTCCTGATTCACCATCTGGAACCACGGATCAGCATATTCTCAAACTGCTAACTTTTAAAAAGCAAGAGTCTGGGATGGTTGAGGAAGGGTGTTATTCATATTACAAGTTCTCCATAGACAACGATATATGGGATATAATTTCTTCCGGAAATGCTAGAGTTGAAGTAAAGCTTGAAGTGGAGACTAATGACGGAGATACAAACATTTACATTTCGAAGCATCCTCTCATATTTCCTACTCAACACCAGCATTCTTGGTCTTCCCATGATATTGGTTCAAAGGTTCTAATCCTTAGCTCTAAAGATAAGAACTTTGGGGCTGGTACTTATAGCATTGGAGTTTATGGATTTAAGGGAACTTCCAAGTACCAAATCTCAGTGAGTATTGAGGATGTCTCAAACCATAAGGTGGGTAAACACGAAGTCTCTTCATCGAAATTTTCAGATCAGGATACTGTCGAATGTCGGAATTGCAAGCATTACATACCCACTCGAACTATAGCTTTGCACGAAGCTTATTGCAGTAGACATAATATTGTCTGCCAGCATGCTGGCTGTGGCGTGGTCCTTAGAGTTGAAGAGGCCAAGAACCATGTGCATTGTGATAAATGCGGACAATCTCTTCAGAAGAGTGAGATGGAGAAACACGATAAGGTGTTCCACGAACCACTCAATTGTTCTTGCGGGGTAGTCCTAGAGAAAGAAGAGATGGTAAGCAAAAATCTCCATGATGGTAAACTGATGAATCCCTTTAGTATTCATTAATATGTGGATCTTATTACGGTCCAAATGAGTAAGCAAAGATCTCCGTGATGGTAAACTGAGATGTTTCATGTATTTTTTACAGGTTCGTCATCAAGCGTCGGTTTGCCCGCTGCGCCTGATAACATGTCAGTTCTGTGGGGACATGGTTCAAGCTGGAAATTCTGCCATTGACATCCGAGATCGAATGAGAGGACTATCTGAGCATGAAAGCATTTGTGGGTCTAGAACAGCAACATGTGATTCATGTGGACGTGCTGTGATGTTGAAGGAGATGGACATCCACCAAGTCGCTGTTCATCAAAAGAGTTAATATCTGCTGCAACAGCTGCGGGATATATTTGTGGTCATGGCTGGAAATTTGCACAGAGATGCAACTCGAACCCGTTGAATAAACTGGTGAGTTCAAACATATGAAGGTTTGCTACGGTTTTATCAAGTTCATTATTATTTTTTGTGCAACCCCAGCGAAGAATTTGAAGATTGTGATTAATATCAATGTGGCAATGACATTATGAAATTAATGTATGATAGTGCTTGGAATTTGATGTACAGTAGTGCTTGAAACTCGGTAAGATGCATTTTGAATTATCAAAGCAGTAATTCATTAAATTTGAAGTGCCCCGCCCCATTTATACTTAATCAGCTGAG

mRNA sequence

TAATCACTGAATAAGAGTAGCGACAAAATTTCCTACTTTTGAGAGTGGATACGCTGCGTTTTGGATTTGGGCCTTGGTGTGGGAAATGGGCCGGGCCATCGAGTGGAAAGGAAAGGCCCAGAAGGTTACAGAACCAAACCCAATTGGTGCCCATCTACCGTCCATCTTTGACCGTCAAAATCCGATTTGGTAATTCTGCCGGAAGGCGGAAGCTTTCGTCTCCGTTTCCTCACCAGCAAATTCATAAATACAGTACAGCCAACTCCGCATTCTTCAATTTGTTTCGCAATTTCTGATCGGAGACAAATCTTACACCGGGAGAGATTCAGGTAAAAAGAGGAGAGGGCACAGATTTTGGAAGAGATGGATTTCGAGCTTAGAAGGGCGAGAGAGAAGCTCGAAAAGGAGCAGAAGGAGAGGAAGGAGATGGCTCGGCGGAGAGTCGAGAGAGAGCGCAAAGCTAAAGAAGAGGCGCGGAAGCAACGAGAATCCATTGAAGCGGCTCAACTTTCTAGACGACTTGATGCTGCCGAGGCCCGAATTAAGGCTGAGCAACTGATGGAAGAAACTTTGCTTGCTGGAAGAGGAATCACTTTTTACCGTCTCTTAGAAGCCATACCTTATCAGGGTAATGGGGACAAGATCAAGCTGCCGCCTTCTGGTTTCACAGAATTGTCCGATCAAAGTGCTTTTGATAAGGGACCTGTGTACTTCCAACTGTCAGTAGTTCATCAAGAAGGTACTTCCAACACAAACATTATGGAGGAAAAGACTCACAGAATCACCCATTCAGGTGTTTTGGAGTTCACTGCAGCTGAAGGTTTTGTTGAGCTACCTCCTCATGTTTGGCGAAATTTATTTCTGGACGACTCTTCAAAGAAGCCACTAATTGAAGTACGTTATGTGTGGCTACCCAAAGGAACCTATGCAAAGCTTCAACCAGAAGGCATAGGCTTTTTGGACTTGCCTAATCATAAAGCTATCCTTGAGACAAGCCTTCGTCAGCATGCCACTCTTTCTCAAGGTGACATACTGACTGTCGTCTACAGTGAGCTTACATATAAGTTAAATGTTCTTGAGTTGAAACCATCTTCTAGTATATCTGTTCTAGAAACGGATATTGAGGTTGATATAGTTGGTCCTGATTCACCATCTGGAACCACGGATCAGCATATTCTCAAACTGCTAACTTTTAAAAAGCAAGAGTCTGGGATGGTTGAGGAAGGGTGTTATTCATATTACAAGTTCTCCATAGACAACGATATATGGGATATAATTTCTTCCGGAAATGCTAGAGTTGAAGTAAAGCTTGAAGTGGAGACTAATGACGGAGATACAAACATTTACATTTCGAAGCATCCTCTCATATTTCCTACTCAACACCAGCATTCTTGGTCTTCCCATGATATTGGTTCAAAGGTTCTAATCCTTAGCTCTAAAGATAAGAACTTTGGGGCTGGTACTTATAGCATTGGAGTTTATGGATTTAAGGGAACTTCCAAGTACCAAATCTCAGTGAGTATTGAGGATGTCTCAAACCATAAGGTGGGTAAACACGAAGTCTCTTCATCGAAATTTTCAGATCAGGATACTGTCGAATGTCGGAATTGCAAGCATTACATACCCACTCGAACTATAGCTTTGCACGAAGCTTATTGCAGTAGACATAATATTGTCTGCCAGCATGCTGGCTGTGGCGTGGTCCTTAGAGTTGAAGAGGCCAAGAACCATGTGCATTGTGATAAATGCGGACAATCTCTTCAGAAGAGTGAGATGGAGAAACACGATAAGGTGTTCCACGAACCACTCAATTGTTCTTGCGGGGTAGTCCTAGAGAAAGAAGAGATGGTTCGTCATCAAGCGTCGGTTTGCCCGCTGCGCCTGATAACATGTCAGTTCTGTGGGGACATGGTTCAAGCTGGAAATTCTGCCATTGACATCCGAGATCGAATGAGAGGACTATCTGAGCATGAAAGCATTTGTGGGTCTAGAACAGCAACATGTGATTCATGTGGACGTGCTGTGATGTTGAAGGAGATGGACATCCACCAAGTCGCTGTTCATCAAAAGAGTTAATATCTGCTGCAACAGCTGCGGGATATATTTGTGGTCATGGCTGGAAATTTGCACAGAGATGCAACTCGAACCCGTTGAATAAACTGGTGAGTTCAAACATATGAAGGTTTGCTACGGTTTTATCAAGTTCATTATTATTTTTTGTGCAACCCCAGCGAAGAATTTGAAGATTGTGATTAATATCAATGTGGCAATGACATTATGAAATTAATGTATGATAGTGCTTGGAATTTGATGTACAGTAGTGCTTGAAACTCGGTAAGATGCATTTTGAATTATCAAAGCAGTAATTCATTAAATTTGAAGTGCCCCGCCCCATTTATACTTAATCAGCTGAG

Coding sequence (CDS)

ATGGATTTCGAGCTTAGAAGGGCGAGAGAGAAGCTCGAAAAGGAGCAGAAGGAGAGGAAGGAGATGGCTCGGCGGAGAGTCGAGAGAGAGCGCAAAGCTAAAGAAGAGGCGCGGAAGCAACGAGAATCCATTGAAGCGGCTCAACTTTCTAGACGACTTGATGCTGCCGAGGCCCGAATTAAGGCTGAGCAACTGATGGAAGAAACTTTGCTTGCTGGAAGAGGAATCACTTTTTACCGTCTCTTAGAAGCCATACCTTATCAGGGTAATGGGGACAAGATCAAGCTGCCGCCTTCTGGTTTCACAGAATTGTCCGATCAAAGTGCTTTTGATAAGGGACCTGTGTACTTCCAACTGTCAGTAGTTCATCAAGAAGGTACTTCCAACACAAACATTATGGAGGAAAAGACTCACAGAATCACCCATTCAGGTGTTTTGGAGTTCACTGCAGCTGAAGGTTTTGTTGAGCTACCTCCTCATGTTTGGCGAAATTTATTTCTGGACGACTCTTCAAAGAAGCCACTAATTGAAGTACGTTATGTGTGGCTACCCAAAGGAACCTATGCAAAGCTTCAACCAGAAGGCATAGGCTTTTTGGACTTGCCTAATCATAAAGCTATCCTTGAGACAAGCCTTCGTCAGCATGCCACTCTTTCTCAAGGTGACATACTGACTGTCGTCTACAGTGAGCTTACATATAAGTTAAATGTTCTTGAGTTGAAACCATCTTCTAGTATATCTGTTCTAGAAACGGATATTGAGGTTGATATAGTTGGTCCTGATTCACCATCTGGAACCACGGATCAGCATATTCTCAAACTGCTAACTTTTAAAAAGCAAGAGTCTGGGATGGTTGAGGAAGGGTGTTATTCATATTACAAGTTCTCCATAGACAACGATATATGGGATATAATTTCTTCCGGAAATGCTAGAGTTGAAGTAAAGCTTGAAGTGGAGACTAATGACGGAGATACAAACATTTACATTTCGAAGCATCCTCTCATATTTCCTACTCAACACCAGCATTCTTGGTCTTCCCATGATATTGGTTCAAAGGTTCTAATCCTTAGCTCTAAAGATAAGAACTTTGGGGCTGGTACTTATAGCATTGGAGTTTATGGATTTAAGGGAACTTCCAAGTACCAAATCTCAGTGAGTATTGAGGATGTCTCAAACCATAAGGTGGGTAAACACGAAGTCTCTTCATCGAAATTTTCAGATCAGGATACTGTCGAATGTCGGAATTGCAAGCATTACATACCCACTCGAACTATAGCTTTGCACGAAGCTTATTGCAGTAGACATAATATTGTCTGCCAGCATGCTGGCTGTGGCGTGGTCCTTAGAGTTGAAGAGGCCAAGAACCATGTGCATTGTGATAAATGCGGACAATCTCTTCAGAAGAGTGAGATGGAGAAACACGATAAGGTGTTCCACGAACCACTCAATTGTTCTTGCGGGGTAGTCCTAGAGAAAGAAGAGATGGTTCGTCATCAAGCGTCGGTTTGCCCGCTGCGCCTGATAACATGTCAGTTCTGTGGGGACATGGTTCAAGCTGGAAATTCTGCCATTGACATCCGAGATCGAATGAGAGGACTATCTGAGCATGAAAGCATTTGTGGGTCTAGAACAGCAACATGTGATTCATGTGGACGTGCTGTGATGTTGAAGGAGATGGACATCCACCAAGTCGCTGTTCATCAAAAGAGTTAA

Protein sequence

MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARIKAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDNDIWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKDKNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYIPTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHEPLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESICGSRTATCDSCGRAVMLKEMDIHQVAVHQKS
Homology
BLAST of MC04g0215 vs. ExPASy Swiss-Prot
Match: Q92890 (Ubiquitin recognition factor in ER-associated degradation protein 1 OS=Homo sapiens OX=9606 GN=UFD1 PE=1 SV=3)

HSP 1 Score: 114.4 bits (285), Expect = 4.3e-24
Identity = 64/170 (37.65%), Postives = 96/170 (56.47%), Query Frame = 0

Query: 91  GDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGVLEFTA 150
           G KI +PPS   +LS  +     P+ F+L+              + + R+TH GVLEF A
Sbjct: 43  GGKIIMPPSALDQLSRLNI--TYPMLFKLT-------------NKNSDRMTHCGVLEFVA 102

Query: 151 AEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHKAILET 210
            EG   LP  + +NL L++     L++V  V L   TY+K QP+   FLD+ N KA+LE 
Sbjct: 103 DEGICYLPHWMMQNLLLEEGG---LVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLEN 162

Query: 211 SLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           +LR  A L+ GD++ + Y+E  Y+L V+E KP  ++S++E D+ VD   P
Sbjct: 163 ALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAP 194

BLAST of MC04g0215 vs. ExPASy Swiss-Prot
Match: P70362 (Ubiquitin recognition factor in ER-associated degradation protein 1 OS=Mus musculus OX=10090 GN=Ufd1 PE=1 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 4.3e-24
Identity = 64/170 (37.65%), Postives = 96/170 (56.47%), Query Frame = 0

Query: 91  GDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGVLEFTA 150
           G KI +PPS   +LS  +     P+ F+L+              + + R+TH GVLEF A
Sbjct: 43  GGKIIMPPSALDQLSRLNI--TYPMLFKLT-------------NKNSDRMTHCGVLEFVA 102

Query: 151 AEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHKAILET 210
            EG   LP  + +NL L++     L++V  V L   TY+K QP+   FLD+ N KA+LE 
Sbjct: 103 DEGICYLPHWMMQNLLLEEGG---LVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLEN 162

Query: 211 SLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           +LR  A L+ GD++ + Y+E  Y+L V+E KP  ++S++E D+ VD   P
Sbjct: 163 ALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAP 194

BLAST of MC04g0215 vs. ExPASy Swiss-Prot
Match: Q9ES53 (Ubiquitin recognition factor in ER-associated degradation protein 1 OS=Rattus norvegicus OX=10116 GN=Ufd1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.3e-24
Identity = 64/170 (37.65%), Postives = 96/170 (56.47%), Query Frame = 0

Query: 91  GDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGVLEFTA 150
           G KI +PPS   +LS  +     P+ F+L+              + + R+TH GVLEF A
Sbjct: 43  GGKIIMPPSALDQLSRLNI--TYPMLFKLT-------------NKNSDRMTHCGVLEFVA 102

Query: 151 AEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHKAILET 210
            EG   LP  + +NL L++     L++V  V L   TY+K QP+   FLD+ N KA+LE 
Sbjct: 103 DEGICYLPHWMMQNLLLEEGG---LVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLEN 162

Query: 211 SLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           +LR  A L+ GD++ + Y+E  Y+L V+E KP  ++S++E D+ VD   P
Sbjct: 163 ALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAP 194

BLAST of MC04g0215 vs. ExPASy Swiss-Prot
Match: Q55BK0 (Ubiquitin fusion degradation protein 1 homolog OS=Dictyostelium discoideum OX=44689 GN=ufd1 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 6.2e-23
Identity = 68/179 (37.99%), Postives = 101/179 (56.42%), Query Frame = 0

Query: 90  NGDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGVLEFT 149
           +G KI LPPS    LS  +   + P+ F++S          N +  K    +H GVLEF 
Sbjct: 46  SGGKILLPPSALNALSRLNI--QYPMLFEIS----------NPISGKK---SHCGVLEFI 105

Query: 150 AAEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHKAILE 209
           A EG   LP  + +NL L +      I+++   L KGT+ K+QP    F+D+ N KA+LE
Sbjct: 106 AEEGICYLPLWMMQNLQLKEGE---FIDIKNATLAKGTFVKIQPRTSNFIDISNPKAVLE 165

Query: 210 TSLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP-DSPSGTT 268
            SLR+ ATL++ D + + Y+   Y L V+ELKP+++IS++E D+ VD   P DS   T+
Sbjct: 166 NSLRKFATLTKDDEIMIDYNNTKYYLKVVELKPANAISIIEADVSVDFAPPMDSKEATS 206

BLAST of MC04g0215 vs. ExPASy Swiss-Prot
Match: Q9VTF9 (Ubiquitin fusion degradation protein 1 homolog OS=Drosophila melanogaster OX=7227 GN=Ufd1-like PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.3e-22
Identity = 62/170 (36.47%), Postives = 96/170 (56.47%), Query Frame = 0

Query: 91  GDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGVLEFTA 150
           G KI +PPS    L+  +   + P+ F+L          TN+   K  R +H+GVLEF A
Sbjct: 40  GGKIIMPPSALDTLTRLNV--EYPMLFKL----------TNV---KKSRSSHAGVLEFVA 99

Query: 151 AEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHKAILET 210
            EG   LP  +  NL L +     ++ +  V LP  T++K QP    FLD+ N KA+LE 
Sbjct: 100 DEGKCYLPHWMMENLLLGEGD---ILNIESVSLPVATFSKFQPHSTDFLDITNPKAVLEN 159

Query: 211 SLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           +LR  A L++GD++ + Y++  Y+L VLE KP +++S++E D+ V+   P
Sbjct: 160 ALRNFACLTRGDVIAIKYNKKVYELCVLETKPGNAVSIIECDMNVEFEAP 191

BLAST of MC04g0215 vs. NCBI nr
Match: XP_022136227.1 (uncharacterized protein LOC111007975 [Momordica charantia])

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 569/570 (99.82%), Postives = 569/570 (99.82%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI
Sbjct: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS
Sbjct: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEGTSNT IMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY
Sbjct: 121 VVHQEGTSNTTIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD
Sbjct: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI
Sbjct: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE
Sbjct: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC
Sbjct: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTATCDSCGRAVMLKEMDIHQVAVHQKS
Sbjct: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570

BLAST of MC04g0215 vs. NCBI nr
Match: KAG6572282.1 (Ubiquitin recognition factor in ER-associated degradation protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1047 bits (2707), Expect = 0.0
Identity = 520/570 (91.23%), Postives = 549/570 (96.32%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELR AREKLEKEQKERKEMARR+V+RERKAKEEARKQRE+IEAAQLSRRLDAAEARI
Sbjct: 1   MDFELRMAREKLEKEQKERKEMARRKVQRERKAKEEARKQREAIEAAQLSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+QLMEE+LLAGRGITFYR+LEAIPY+GNGDKIKLPPSGFTELSDQ AFDKGPVYFQLS
Sbjct: 61  KADQLMEESLLAGRGITFYRVLEAIPYEGNGDKIKLPPSGFTELSDQGAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEG S++++ EEKTHR T SGVLEFTAAEGFVELPPHVWRNLFLDDSS KPL+EVRY
Sbjct: 121 VVHQEGPSDSDVTEEKTHRTTRSGVLEFTAAEGFVELPPHVWRNLFLDDSSTKPLVEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPN KAILETSLRQHATLSQGDILTVVY ELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGIGFLDLPNPKAILETSLRQHATLSQGDILTVVYGELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGT DQH+LKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGTMDQHVLKLLTFKKQESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWD+ISSGNARVEVK EVETN+GDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLIL+S+D
Sbjct: 301 IWDVISSGNARVEVKFEVETNEGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILNSRD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFGAGTYSIGVYGFKGTSKYQI+VSIEDV+NHKVG+ EVSSS F DQDT ECRNCKHYI
Sbjct: 361 KNFGAGTYSIGVYGFKGTSKYQITVSIEDVTNHKVGRQEVSSSTFIDQDTAECRNCKHYI 420

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           P+RTIALHEAYCSRHN VC+HA CGVVLRVEEAKNHVHCDKCGQ+LQKSEMEKHDKVFH 
Sbjct: 421 PSRTIALHEAYCSRHNFVCKHADCGVVLRVEEAKNHVHCDKCGQALQKSEMEKHDKVFHV 480

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PL C+CGVVLEKEEMV+HQA VCPLRLITCQFCGDMVQAG+SA+DIRDR+RGLSEHESIC
Sbjct: 481 PLKCACGVVLEKEEMVQHQALVCPLRLITCQFCGDMVQAGSSAMDIRDRLRGLSEHESIC 540

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTA CDSCGRAVMLKEMDIHQ+AVHQKS
Sbjct: 541 GSRTAPCDSCGRAVMLKEMDIHQIAVHQKS 570

BLAST of MC04g0215 vs. NCBI nr
Match: XP_022952609.1 (uncharacterized protein LOC111455245 [Cucurbita moschata])

HSP 1 Score: 1045 bits (2701), Expect = 0.0
Identity = 519/570 (91.05%), Postives = 548/570 (96.14%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELR AREKLEKEQKERKEMARR+V+RERKAKEEARKQRE+IEAAQLSRRLDAAEARI
Sbjct: 1   MDFELRMAREKLEKEQKERKEMARRKVQRERKAKEEARKQREAIEAAQLSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+QLMEE+LLAGRGITFYR+LEAIPY+GNGDKIKLPPSGFTELSDQ AFDKGPVYFQLS
Sbjct: 61  KADQLMEESLLAGRGITFYRVLEAIPYEGNGDKIKLPPSGFTELSDQGAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEG S++++ EEKTHR T SGVLEFTAAEGFVELPPHVWR LFLDDSS KPL+EVRY
Sbjct: 121 VVHQEGPSDSDVTEEKTHRTTRSGVLEFTAAEGFVELPPHVWRTLFLDDSSTKPLVEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPN KAILETSLRQHATLSQGDILTVVY ELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGIGFLDLPNPKAILETSLRQHATLSQGDILTVVYGELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGT DQH+LKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGTMDQHVLKLLTFKKQESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWD+ISSGNARVEVK EVETN+GDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLIL+S+D
Sbjct: 301 IWDVISSGNARVEVKFEVETNEGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILNSRD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFGAGTYSIGVYGFKGTSKYQI+VSIEDV+NHKVG+ EVSSS F DQDT ECRNCKHYI
Sbjct: 361 KNFGAGTYSIGVYGFKGTSKYQITVSIEDVTNHKVGRQEVSSSTFIDQDTAECRNCKHYI 420

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           P+RTIALHEAYCSRHN VC+HA CGVVLRVEEAKNHVHCDKCGQ+LQKSEMEKHDKVFH 
Sbjct: 421 PSRTIALHEAYCSRHNFVCKHADCGVVLRVEEAKNHVHCDKCGQALQKSEMEKHDKVFHV 480

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PL C+CGVVLEKEEMV+HQA VCPLRLITCQFCGDMVQAG+SA+DIRDR+RGLSEHESIC
Sbjct: 481 PLKCACGVVLEKEEMVQHQALVCPLRLITCQFCGDMVQAGSSAMDIRDRLRGLSEHESIC 540

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTA CDSCGRAVMLKEMDIHQ+AVHQKS
Sbjct: 541 GSRTAPCDSCGRAVMLKEMDIHQIAVHQKS 570

BLAST of MC04g0215 vs. NCBI nr
Match: KAG7011898.1 (Ubiquitin recognition factor in ER-associated degradation protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1043 bits (2698), Expect = 0.0
Identity = 518/570 (90.88%), Postives = 548/570 (96.14%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELR AREKLEKEQKERKEMARR+V+RERKAKEEARKQRE+IEAAQLSRRLDAAEARI
Sbjct: 3   MDFELRMAREKLEKEQKERKEMARRKVQRERKAKEEARKQREAIEAAQLSRRLDAAEARI 62

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+QLMEE+LLAGRGITFYR+LEAIPY+GNGDKIKLPPSGFTELSDQ AFDKGPVYFQLS
Sbjct: 63  KADQLMEESLLAGRGITFYRVLEAIPYEGNGDKIKLPPSGFTELSDQGAFDKGPVYFQLS 122

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEG S++++ EEKTHR T SGVLEFTAAEGFVELPPHVWRNLFLDDSS KPL+EVRY
Sbjct: 123 VVHQEGPSDSDVTEEKTHRTTRSGVLEFTAAEGFVELPPHVWRNLFLDDSSTKPLVEVRY 182

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPN KAILETSLRQHATLSQGDILTVVY ELTYKLNVLEL
Sbjct: 183 VWLPKGTYAKLQPEGIGFLDLPNPKAILETSLRQHATLSQGDILTVVYGELTYKLNVLEL 242

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGT DQH+LKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 243 KPSSSISVLETDIEVDIVGPDSPSGTMDQHVLKLLTFKKQESGMVEEGCYSYYKFSIDND 302

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWD+ISSGNARVEVK EVETN+GDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLIL+S+D
Sbjct: 303 IWDVISSGNARVEVKFEVETNEGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILNSRD 362

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFGAGTYSIGVYGFKGTSKYQI+VSIEDV+NHKVG+ EVSSS F DQDT ECRNCKHYI
Sbjct: 363 KNFGAGTYSIGVYGFKGTSKYQITVSIEDVTNHKVGRQEVSSSTFIDQDTAECRNCKHYI 422

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           P+RTIALHEAYCSRHN VC+HA CGVVLRVEEAKNHVHCDKCGQ+LQKSEMEKH+KVFH 
Sbjct: 423 PSRTIALHEAYCSRHNFVCKHADCGVVLRVEEAKNHVHCDKCGQALQKSEMEKHEKVFHV 482

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PL C+CGVVLEKEEMV+HQA VCPLRLITCQFCGDMVQAG+SA+DIRDR+RGLSEHESIC
Sbjct: 483 PLKCACGVVLEKEEMVQHQALVCPLRLITCQFCGDMVQAGSSAMDIRDRLRGLSEHESIC 542

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTA CDSCGRAVMLKEMDIH +AVHQKS
Sbjct: 543 GSRTAPCDSCGRAVMLKEMDIHHIAVHQKS 572

BLAST of MC04g0215 vs. NCBI nr
Match: XP_023554439.1 (uncharacterized protein LOC111811676 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 517/570 (90.70%), Postives = 547/570 (95.96%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELR AREKLEKEQKERKEMARR+V+RERKAKEEARKQRE+IEAAQLSRRLDAAEARI
Sbjct: 1   MDFELRMAREKLEKEQKERKEMARRKVQRERKAKEEARKQREAIEAAQLSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+QLMEE+LLAGRGITFYR+LEAIPY+GNGDKIKLPPSGFTELSDQ AFDKGPVYFQLS
Sbjct: 61  KADQLMEESLLAGRGITFYRVLEAIPYEGNGDKIKLPPSGFTELSDQGAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEG S++++ EEKTHR T SGVLEFTAAEG VELPPHVWRNLFLDDSS KPL+EVRY
Sbjct: 121 VVHQEGPSDSDVTEEKTHRTTRSGVLEFTAAEGIVELPPHVWRNLFLDDSSTKPLVEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPN KAILETSLRQHATLSQGD LTVVY ELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGIGFLDLPNPKAILETSLRQHATLSQGDNLTVVYGELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGT DQH+LKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGTMDQHVLKLLTFKKQESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWD+ISSGNARVEVK EVETN+GDTN+YISKHPLIFPTQHQHSWSSHDIGSKVLIL+S+D
Sbjct: 301 IWDVISSGNARVEVKFEVETNEGDTNVYISKHPLIFPTQHQHSWSSHDIGSKVLILNSRD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFGAGTYSIGVYGFKGTSKYQI+VSIEDV+NHKVG+ EVSSS F DQDT ECRNCKHYI
Sbjct: 361 KNFGAGTYSIGVYGFKGTSKYQITVSIEDVTNHKVGRQEVSSSTFIDQDTAECRNCKHYI 420

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           P+RTIALHEAYCSRHN VC+HA CGVVLRVEEAKNHVHCDKCGQ+LQKSEMEKHDKVFH 
Sbjct: 421 PSRTIALHEAYCSRHNFVCKHADCGVVLRVEEAKNHVHCDKCGQALQKSEMEKHDKVFHV 480

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PL C+CGVVLEKEEMV+HQA VCPLRLITCQFCGDMVQAG+SA+DIRDR+RGLSEHESIC
Sbjct: 481 PLKCACGVVLEKEEMVQHQALVCPLRLITCQFCGDMVQAGSSAMDIRDRLRGLSEHESIC 540

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTA CDSCGRAVMLKEMDIHQ+AVHQKS
Sbjct: 541 GSRTAPCDSCGRAVMLKEMDIHQIAVHQKS 570

BLAST of MC04g0215 vs. ExPASy TrEMBL
Match: A0A6J1C4Z7 (uncharacterized protein LOC111007975 OS=Momordica charantia OX=3673 GN=LOC111007975 PE=3 SV=1)

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 569/570 (99.82%), Postives = 569/570 (99.82%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI
Sbjct: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS
Sbjct: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEGTSNT IMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY
Sbjct: 121 VVHQEGTSNTTIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD
Sbjct: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI
Sbjct: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE
Sbjct: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC
Sbjct: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTATCDSCGRAVMLKEMDIHQVAVHQKS
Sbjct: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570

BLAST of MC04g0215 vs. ExPASy TrEMBL
Match: A0A6J1GM69 (uncharacterized protein LOC111455245 OS=Cucurbita moschata OX=3662 GN=LOC111455245 PE=3 SV=1)

HSP 1 Score: 1045 bits (2701), Expect = 0.0
Identity = 519/570 (91.05%), Postives = 548/570 (96.14%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELR AREKLEKEQKERKEMARR+V+RERKAKEEARKQRE+IEAAQLSRRLDAAEARI
Sbjct: 1   MDFELRMAREKLEKEQKERKEMARRKVQRERKAKEEARKQREAIEAAQLSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+QLMEE+LLAGRGITFYR+LEAIPY+GNGDKIKLPPSGFTELSDQ AFDKGPVYFQLS
Sbjct: 61  KADQLMEESLLAGRGITFYRVLEAIPYEGNGDKIKLPPSGFTELSDQGAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEG S++++ EEKTHR T SGVLEFTAAEGFVELPPHVWR LFLDDSS KPL+EVRY
Sbjct: 121 VVHQEGPSDSDVTEEKTHRTTRSGVLEFTAAEGFVELPPHVWRTLFLDDSSTKPLVEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPN KAILETSLRQHATLSQGDILTVVY ELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGIGFLDLPNPKAILETSLRQHATLSQGDILTVVYGELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGT DQH+LKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGTMDQHVLKLLTFKKQESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWD+ISSGNARVEVK EVETN+GDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLIL+S+D
Sbjct: 301 IWDVISSGNARVEVKFEVETNEGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILNSRD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFGAGTYSIGVYGFKGTSKYQI+VSIEDV+NHKVG+ EVSSS F DQDT ECRNCKHYI
Sbjct: 361 KNFGAGTYSIGVYGFKGTSKYQITVSIEDVTNHKVGRQEVSSSTFIDQDTAECRNCKHYI 420

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           P+RTIALHEAYCSRHN VC+HA CGVVLRVEEAKNHVHCDKCGQ+LQKSEMEKHDKVFH 
Sbjct: 421 PSRTIALHEAYCSRHNFVCKHADCGVVLRVEEAKNHVHCDKCGQALQKSEMEKHDKVFHV 480

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PL C+CGVVLEKEEMV+HQA VCPLRLITCQFCGDMVQAG+SA+DIRDR+RGLSEHESIC
Sbjct: 481 PLKCACGVVLEKEEMVQHQALVCPLRLITCQFCGDMVQAGSSAMDIRDRLRGLSEHESIC 540

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTA CDSCGRAVMLKEMDIHQ+AVHQKS
Sbjct: 541 GSRTAPCDSCGRAVMLKEMDIHQIAVHQKS 570

BLAST of MC04g0215 vs. ExPASy TrEMBL
Match: A0A6J1HVA0 (uncharacterized protein LOC111468160 OS=Cucurbita maxima OX=3661 GN=LOC111468160 PE=3 SV=1)

HSP 1 Score: 1039 bits (2687), Expect = 0.0
Identity = 518/570 (90.88%), Postives = 549/570 (96.32%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELR AREKLEKEQKERKEMARR+V+RERKAKEEARKQRE+IEAAQLSRRLDAAEARI
Sbjct: 1   MDFELRMAREKLEKEQKERKEMARRKVQRERKAKEEARKQREAIEAAQLSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+Q+MEE+LLAGRGITFYR+LEAIPY+GNGDKIKLPPSGFTELSDQ AFDKGPVYFQLS
Sbjct: 61  KADQIMEESLLAGRGITFYRVLEAIPYEGNGDKIKLPPSGFTELSDQGAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQ G S+++I EEKTHR T SGVLEFTAAEGFVELPPHVWRNLFLDDSS KPL+EVRY
Sbjct: 121 VVHQ-GPSDSDITEEKTHRTTRSGVLEFTAAEGFVELPPHVWRNLFLDDSSTKPLVEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEGIGFLDLPN KAILETSLRQHATLSQGDILTVVY ELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGIGFLDLPNPKAILETSLRQHATLSQGDILTVVYGELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSGT DQH+LKLLTFKKQESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGTMDQHVLKLLTFKKQESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWD+ISSGNARVEVK EVETN+GDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLIL+S+D
Sbjct: 301 IWDVISSGNARVEVKFEVETNEGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILNSRD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKVGKHEVSSSKFSDQDTVECRNCKHYI 420
           KNFG+GTYSIGVYGFKGTSKYQI+VSIEDV+NHKVG+ EVSSS F DQDT ECRNCKHY+
Sbjct: 361 KNFGSGTYSIGVYGFKGTSKYQITVSIEDVTNHKVGRQEVSSSTFIDQDTAECRNCKHYM 420

Query: 421 PTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFHE 480
           P+RTIALHEAYCSRHNIVC+HA CGVVLRVEEAKNHVHCDKCGQ+LQKSEMEKHDKVFH 
Sbjct: 421 PSRTIALHEAYCSRHNIVCKHADCGVVLRVEEAKNHVHCDKCGQALQKSEMEKHDKVFHV 480

Query: 481 PLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESIC 540
           PL C+CGVVLEKEEMV+HQA VCPLRLITCQFCGDMVQAG+SA+DIRDR+RGLSEHESIC
Sbjct: 481 PLKCACGVVLEKEEMVQHQALVCPLRLITCQFCGDMVQAGSSAMDIRDRLRGLSEHESIC 540

Query: 541 GSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           GSRTA CDSCGRAVMLKEMDIHQ+AVHQKS
Sbjct: 541 GSRTAPCDSCGRAVMLKEMDIHQIAVHQKS 569

BLAST of MC04g0215 vs. ExPASy TrEMBL
Match: A0A0A0K6T6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G041950 PE=3 SV=1)

HSP 1 Score: 1025 bits (2651), Expect = 0.0
Identity = 512/571 (89.67%), Postives = 541/571 (94.75%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELRRAREKLEKEQKERKEMARR+VERERKAKEEARK RE+IEAAQ+SRRLDAAEARI
Sbjct: 1   MDFELRRAREKLEKEQKERKEMARRKVERERKAKEEARKLREAIEAAQVSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+QLMEETLLAGRGI+FYR+LEAIPYQGNGDKIKL PS FTELSDQ AFDKGPVYFQLS
Sbjct: 61  KADQLMEETLLAGRGISFYRILEAIPYQGNGDKIKLSPSSFTELSDQGAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           VVHQEG SN+ + +EKTHR THSGVLEFTA EGFVELPPHVW+NLFLD+S  KPL+EVRY
Sbjct: 121 VVHQEGPSNSEVTKEKTHRATHSGVLEFTADEGFVELPPHVWQNLFLDESLTKPLVEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEG+GFLDLPNHKAILETSLRQHATLSQGDILTVVY ELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGLGFLDLPNHKAILETSLRQHATLSQGDILTVVYGELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSG  DQ +LKLLTFKK ESGMVEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGAMDQRVLKLLTFKKHESGMVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWDIISSGNARVEVKLEVETN+GDTNIYISKHPLIFP+QHQHSWSSHDIGSKVLIL+SKD
Sbjct: 301 IWDIISSGNARVEVKLEVETNEGDTNIYISKHPLIFPSQHQHSWSSHDIGSKVLILNSKD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKV-GKHEVSSSKFSDQDTVECRNCKHY 420
           KNFG GTYSIGVYGFKGTSKYQI VS E+ +NHKV GK EVSSS  + QDTVECRNCKHY
Sbjct: 361 KNFGPGTYSIGVYGFKGTSKYQIQVSFEEATNHKVVGKQEVSSSTSTHQDTVECRNCKHY 420

Query: 421 IPTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFH 480
           IP+RTIALHEAYCSRHNIVCQHA CGVVLRVEEA+NHVHC+KCGQ+LQKSEMEKHDKVFH
Sbjct: 421 IPSRTIALHEAYCSRHNIVCQHADCGVVLRVEEARNHVHCEKCGQALQKSEMEKHDKVFH 480

Query: 481 EPLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESI 540
            PL C+CG+VLEKEEMVRHQASVCPLRLI CQFCGDMVQAGNSA+DIRDR+RGLSEHESI
Sbjct: 481 VPLKCACGLVLEKEEMVRHQASVCPLRLIACQFCGDMVQAGNSAMDIRDRLRGLSEHESI 540

Query: 541 CGSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           CGSRTA CDSCGRAVMLKEMDIHQ+AVHQKS
Sbjct: 541 CGSRTAPCDSCGRAVMLKEMDIHQIAVHQKS 571

BLAST of MC04g0215 vs. ExPASy TrEMBL
Match: A0A5D3C5A8 (PRLI-interacting factor K family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G004440 PE=3 SV=1)

HSP 1 Score: 999 bits (2582), Expect = 0.0
Identity = 499/571 (87.39%), Postives = 534/571 (93.52%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELRRAREKLEKEQKERKEMARR+VERERKAKEEARK RE+IEA Q+SRRLDAAEARI
Sbjct: 1   MDFELRRAREKLEKEQKERKEMARRKVERERKAKEEARKLREAIEAVQVSRRLDAAEARI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+QLMEETLLAGRGI+FYR+L AIPYQGNGDKIKL PS FTELSDQ AFDKGPVYFQLS
Sbjct: 61  KADQLMEETLLAGRGISFYRILVAIPYQGNGDKIKLSPSSFTELSDQGAFDKGPVYFQLS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRY 180
           V+H+EG SN+ + +EKTHR THSGVLEFTA EG VELPPHVW+NL LD+S  KPL+EVRY
Sbjct: 121 VLHREGPSNSEVTKEKTHRATHSGVLEFTADEGSVELPPHVWQNLSLDESLTKPLVEVRY 180

Query: 181 VWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLEL 240
           VWLPKGTYAKLQPEG+GFLDLPNHKAILETSLRQHATLS+GD LTVVY ELTYKLNVLEL
Sbjct: 181 VWLPKGTYAKLQPEGLGFLDLPNHKAILETSLRQHATLSEGDTLTVVYGELTYKLNVLEL 240

Query: 241 KPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDND 300
           KPSSSISVLETDIEVDIVGPDSPSG  DQ +LKLLTFKK ESG+VEEGCYSYYKFSIDND
Sbjct: 241 KPSSSISVLETDIEVDIVGPDSPSGPMDQRVLKLLTFKKHESGVVEEGCYSYYKFSIDND 300

Query: 301 IWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSKD 360
           IWDIISSGNARVEVKLEVETN+GDTNIYISKHPLIFP+QHQH WSSHDIGSKVLIL+SKD
Sbjct: 301 IWDIISSGNARVEVKLEVETNEGDTNIYISKHPLIFPSQHQHLWSSHDIGSKVLILNSKD 360

Query: 361 KNFGAGTYSIGVYGFKGTSKYQISVSIEDVSNHKV-GKHEVSSSKFSDQDTVECRNCKHY 420
           KNFG GTYSIGVYGFKGTSKYQI VS+E+ +NHKV GK EVSSS  + QDTVECRNCKHY
Sbjct: 361 KNFGPGTYSIGVYGFKGTSKYQIQVSVEEGTNHKVVGKQEVSSSTSTHQDTVECRNCKHY 420

Query: 421 IPTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVFH 480
           IP+RTIALHEAYC RHNIVCQH  CGVVLRVEEA+NHVHC+KCGQ+LQKSEMEKHDKVFH
Sbjct: 421 IPSRTIALHEAYCIRHNIVCQHGDCGVVLRVEEARNHVHCEKCGQALQKSEMEKHDKVFH 480

Query: 481 EPLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHESI 540
            PL C+CG+VLEKEEMVRHQASVCPLRLITCQFCGDMVQAG+SA+DIRDR+RGLSEHESI
Sbjct: 481 VPLKCACGLVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGSSAMDIRDRLRGLSEHESI 540

Query: 541 CGSRTATCDSCGRAVMLKEMDIHQVAVHQKS 570
           CGSRTA CDSCGRAVMLKEMDIHQ+AVH KS
Sbjct: 541 CGSRTAPCDSCGRAVMLKEMDIHQIAVHPKS 571

BLAST of MC04g0215 vs. TAIR 10
Match: AT4G15420.1 (Ubiquitin fusion degradation UFD1 family protein )

HSP 1 Score: 749.2 bits (1933), Expect = 2.4e-216
Identity = 376/572 (65.73%), Postives = 457/572 (79.90%), Query Frame = 0

Query: 1   MDFELRRAREKLEKEQKERKEMARRRVERERKAKEEARKQRESIEAAQLSRRLDAAEARI 60
           MDFELR A+EKLE+EQ+ERK+ A+ ++ERE+K+KE A KQRE+IEAAQ +RRLDA EA+I
Sbjct: 1   MDFELRSAKEKLEREQRERKQRAKLKLEREKKSKEAAIKQREAIEAAQRARRLDAIEAQI 60

Query: 61  KAEQLMEETLLAGRGITFYRLLEAIPYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLS 120
           KA+Q M+E+L++G GI F R+ +A+ +QGNGDKIKLPPS FTELSDQ AFDKGP+YF+LS
Sbjct: 61  KADQHMQESLVSGDGIVFERVFQAVSFQGNGDKIKLPPSCFTELSDQGAFDKGPLYFELS 120

Query: 121 VVHQEGTSNTNIMEEKTHRITHSGVLEFTAAEGFVELPPHVWRNLF-LDDSSKKPLIEVR 180
           VV       T          THSGVLEFTA +G + LPPHVW NLF   D    PL+E+R
Sbjct: 121 VVDHADNKKT----------THSGVLEFTAEDGTIGLPPHVWSNLFSTHDPMDVPLVEIR 180

Query: 181 YVWLPKGTYAKLQPEGIGFLDLPNHKAILETSLRQHATLSQGDILTVVYSELTYKLNVLE 240
           Y+ LPKG+YAKLQP+ +GF DLPNHKAILET LRQHATLS  D+L V Y +++YKL VLE
Sbjct: 181 YIRLPKGSYAKLQPDNLGFSDLPNHKAILETILRQHATLSLDDVLLVNYGQVSYKLQVLE 240

Query: 241 LKPSSSISVLETDIEVDIVGPDSPSGTTDQHILKLLTFKKQESGMVEEGCYSYYKFSIDN 300
           L+P++SISVLETDIEVDIV PD  S   +QH+LK L + K ESG VEEG Y YYKF ID 
Sbjct: 241 LRPATSISVLETDIEVDIVSPDIVSDQPNQHVLKPLQYGKSESGTVEEGRYDYYKFVIDE 300

Query: 301 DIWDIISSGNARVEVKLEVETNDGDTNIYISKHPLIFPTQHQHSWSSHDIGSKVLILSSK 360
              + + +G+ +V VK++VE    DT++Y+SKHP++FP+ +QH WSSHD+GSK LIL SK
Sbjct: 301 ATVEKVMAGSVKVIVKVDVEKVGADTDLYVSKHPVLFPSLNQHEWSSHDVGSKTLILVSK 360

Query: 361 DKNFGAGTYSIGVYGFKGTSKYQISVSI-EDVSNHKVGKHEVSSSKFSDQDTVECRNCKH 420
           ++   +GTYSIGVYGFKGT KYQ+SV + E +   KVG+  VSSS  SD DTVECRNCKH
Sbjct: 361 ERALSSGTYSIGVYGFKGTVKYQVSVLVQESIDGAKVGERAVSSS--SDVDTVECRNCKH 420

Query: 421 YIPTRTIALHEAYCSRHNIVCQHAGCGVVLRVEEAKNHVHCDKCGQSLQKSEMEKHDKVF 480
            IP+R+IALHE YCSRHN+VC H GCG+VLRVEEAKNH+HC+KCG++LQ +EMEKH KVF
Sbjct: 421 SIPSRSIALHEVYCSRHNVVCNHHGCGIVLRVEEAKNHLHCEKCGKALQPTEMEKHLKVF 480

Query: 481 HEPLNCSCGVVLEKEEMVRHQASVCPLRLITCQFCGDMVQAGNSAIDIRDRMRGLSEHES 540
           HEPL C CG+VLEKE+MV+HQ   CPLRLI C+FCGDMV+AGNSA D RDRMRG+SEHES
Sbjct: 481 HEPLTCGCGIVLEKEQMVQHQGKDCPLRLIACRFCGDMVEAGNSAADTRDRMRGMSEHES 540

Query: 541 ICGSRTATCDSCGRAVMLKEMDIHQVAVHQKS 571
            CGSRTA CDSCGR+VMLK+MDIHQ+AVH KS
Sbjct: 541 TCGSRTAPCDSCGRSVMLKDMDIHQIAVHGKS 560

BLAST of MC04g0215 vs. TAIR 10
Match: AT2G29070.2 (Ubiquitin fusion degradation UFD1 family protein )

HSP 1 Score: 130.2 bits (326), Expect = 5.3e-30
Identity = 76/170 (44.71%), Postives = 105/170 (61.76%), Query Frame = 0

Query: 91  GDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGVLEFTA 150
           GDKI +PPS    L+  S   + P+ FQLS          N+  EKT   +H GVLEFTA
Sbjct: 28  GDKIIMPPSALDRLA--SLHIEYPMLFQLS----------NVSVEKT---SHCGVLEFTA 87

Query: 151 AEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHKAILET 210
            EG V LP  + +N+ L++     +++V+ + L KGTY KLQP    FLD+ N KAILET
Sbjct: 88  DEGLVYLPYWMMQNMSLEEGD---VMQVKNISLVKGTYIKLQPHTQDFLDISNPKAILET 147

Query: 211 SLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           +LR ++ L+ GD + V Y+   Y +NV+E KPSS++S++ETD EVD   P
Sbjct: 148 TLRSYSCLTTGDTIMVPYNNKQYYINVVEAKPSSAVSIIETDCEVDFAPP 179

BLAST of MC04g0215 vs. TAIR 10
Match: AT2G21270.1 (ubiquitin fusion degradation 1 )

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-29
Identity = 73/175 (41.71%), Postives = 102/175 (58.29%), Query Frame = 0

Query: 86  PYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGV 145
           P   +GDKI +PPS    L+  S     P+ F+L         N  I      R+TH GV
Sbjct: 28  PQLESGDKIIMPPSALDRLA--SLHIDYPMLFEL--------RNAGI-----ERVTHCGV 87

Query: 146 LEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHK 205
           LEF A EG + +P  + +NL L +     ++ VR V LPKGTY KLQP    FLD+ N K
Sbjct: 88  LEFIAEEGMIYMPYWMMQNLLLQEGD---IVRVRNVTLPKGTYVKLQPHTTDFLDISNPK 147

Query: 206 AILETSLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           AILET+LR ++ L+ GD + V Y+   Y ++++E KP+++IS++ETD EVD   P
Sbjct: 148 AILETALRNYSCLTSGDSIMVPYNNKKYFIDIVETKPANAISIIETDCEVDFAPP 184

BLAST of MC04g0215 vs. TAIR 10
Match: AT2G21270.2 (ubiquitin fusion degradation 1 )

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-29
Identity = 73/175 (41.71%), Postives = 102/175 (58.29%), Query Frame = 0

Query: 86  PYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGV 145
           P   +GDKI +PPS    L+  S     P+ F+L         N  I      R+TH GV
Sbjct: 28  PQLESGDKIIMPPSALDRLA--SLHIDYPMLFEL--------RNAGI-----ERVTHCGV 87

Query: 146 LEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHK 205
           LEF A EG + +P  + +NL L +     ++ VR V LPKGTY KLQP    FLD+ N K
Sbjct: 88  LEFIAEEGMIYMPYWMMQNLLLQEGD---IVRVRNVTLPKGTYVKLQPHTTDFLDISNPK 147

Query: 206 AILETSLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           AILET+LR ++ L+ GD + V Y+   Y ++++E KP+++IS++ETD EVD   P
Sbjct: 148 AILETALRNYSCLTSGDSIMVPYNNKKYFIDIVETKPANAISIIETDCEVDFAPP 184

BLAST of MC04g0215 vs. TAIR 10
Match: AT2G21270.3 (ubiquitin fusion degradation 1 )

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-29
Identity = 73/175 (41.71%), Postives = 102/175 (58.29%), Query Frame = 0

Query: 86  PYQGNGDKIKLPPSGFTELSDQSAFDKGPVYFQLSVVHQEGTSNTNIMEEKTHRITHSGV 145
           P   +GDKI +PPS    L+  S     P+ F+L         N  I      R+TH GV
Sbjct: 49  PQLESGDKIIMPPSALDRLA--SLHIDYPMLFEL--------RNAGI-----ERVTHCGV 108

Query: 146 LEFTAAEGFVELPPHVWRNLFLDDSSKKPLIEVRYVWLPKGTYAKLQPEGIGFLDLPNHK 205
           LEF A EG + +P  + +NL L +     ++ VR V LPKGTY KLQP    FLD+ N K
Sbjct: 109 LEFIAEEGMIYMPYWMMQNLLLQEGD---IVRVRNVTLPKGTYVKLQPHTTDFLDISNPK 168

Query: 206 AILETSLRQHATLSQGDILTVVYSELTYKLNVLELKPSSSISVLETDIEVDIVGP 261
           AILET+LR ++ L+ GD + V Y+   Y ++++E KP+++IS++ETD EVD   P
Sbjct: 169 AILETALRNYSCLTSGDSIMVPYNNKKYFIDIVETKPANAISIIETDCEVDFAPP 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q928904.3e-2437.65Ubiquitin recognition factor in ER-associated degradation protein 1 OS=Homo sapi... [more]
P703624.3e-2437.65Ubiquitin recognition factor in ER-associated degradation protein 1 OS=Mus muscu... [more]
Q9ES534.3e-2437.65Ubiquitin recognition factor in ER-associated degradation protein 1 OS=Rattus no... [more]
Q55BK06.2e-2337.99Ubiquitin fusion degradation protein 1 homolog OS=Dictyostelium discoideum OX=44... [more]
Q9VTF92.3e-2236.47Ubiquitin fusion degradation protein 1 homolog OS=Drosophila melanogaster OX=722... [more]
Match NameE-valueIdentityDescription
XP_022136227.10.099.82uncharacterized protein LOC111007975 [Momordica charantia][more]
KAG6572282.10.091.23Ubiquitin recognition factor in ER-associated degradation protein 1, partial [Cu... [more]
XP_022952609.10.091.05uncharacterized protein LOC111455245 [Cucurbita moschata][more]
KAG7011898.10.090.88Ubiquitin recognition factor in ER-associated degradation protein 1, partial [Cu... [more]
XP_023554439.10.090.70uncharacterized protein LOC111811676 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1C4Z70.099.82uncharacterized protein LOC111007975 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A6J1GM690.091.05uncharacterized protein LOC111455245 OS=Cucurbita moschata OX=3662 GN=LOC1114552... [more]
A0A6J1HVA00.090.88uncharacterized protein LOC111468160 OS=Cucurbita maxima OX=3661 GN=LOC111468160... [more]
A0A0A0K6T60.089.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G041950 PE=3 SV=1[more]
A0A5D3C5A80.087.39PRLI-interacting factor K family protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
Match NameE-valueIdentityDescription
AT4G15420.12.4e-21665.73Ubiquitin fusion degradation UFD1 family protein [more]
AT2G29070.25.3e-3044.71Ubiquitin fusion degradation UFD1 family protein [more]
AT2G21270.12.0e-2941.71ubiquitin fusion degradation 1 [more]
AT2G21270.22.0e-2941.71ubiquitin fusion degradation 1 [more]
AT2G21270.32.0e-2941.71ubiquitin fusion degradation 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 5..66
NoneNo IPR availableGENE3D3.10.330.10coord: 185..258
e-value: 1.1E-23
score: 84.9
NoneNo IPR availableGENE3D2.60.120.380coord: 279..392
e-value: 1.6E-6
score: 30.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availablePANTHERPTHR12555:SF22UBIQUITIN FUSION DEGRADATION UFD1 FAMILY PROTEINcoord: 1..569
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 478..526
e-value: 2.7E-6
score: 29.6
IPR004854Ubiquitin fusion degradation protein Ufd1-likePFAMPF03152UFD1coord: 90..260
e-value: 6.8E-47
score: 159.1
IPR004854Ubiquitin fusion degradation protein Ufd1-likePANTHERPTHR12555UBIQUITIN FUSION DEGRADATON PROTEIN 1coord: 1..569
IPR042299Ufd1-like, Nn domainGENE3D2.40.40.50coord: 76..183
e-value: 2.7E-17
score: 64.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0215.1MC04g0215.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071712 ER-associated misfolded protein catabolic process
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0034098 VCP-NPL4-UFD1 AAA ATPase complex
molecular_function GO:0031593 polyubiquitin modification-dependent protein binding