MC04g0132 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0132
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Diacylglycerol O-acyltransferase
Location	MC04: 986896 .. 989704 (-)
RNA-Seq Expression	MC04g0132
Synteny	MC04g0132

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCAATTTTCAGATGATTTTGCGACTATAAAATGGGAAGATTCAGTAGCTCACAAACCATCAAGATCAATCCTTCCTTGTTCTAACTTCCAAAGAACCTCCCTCATTTGTAATGTTCAACTCTTTTTAACTTTTTTCTTTTTTCATCTTTCAGTTATTTCTTTGATTTTTTAGAACTTTTGTTTTTCTAATTACTTTTTGCAGGCACTGAATCAACACACATCTTTCATTAATGGAGATTCTTGAAGATTCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGAGTCCTCGAACTCGAATCTCCGATCTCTCCCCCTGACGCCATTTCAATTGTAACTGATGTTCTCCTCCCTGCCAACCCACGCTTTACTTCAATCATGGTACCTCCACTCTGCTCTAAAAATACGAATAAAAAGTTAAAATTACTCATTCAAACTACTGTAATGTCATTTTCTCTCCAATCAACAGGTAAAAGAGAAGTCAACGGGAGAAAGAAAGTGGAAGACAGCTGAAGTTAACCCAAGAGACCACATTTACGTACCGACTTTTCCAAACGACCTCTCGCCTTATGAATACGATGCTTACTTCGACGAATATGCCACGAAAACAGCCACTAAACCCTTCTCCCAAAGCATTCCTTTGTGGGAAATTCACGTTTTCAACTACCCTACAAGCCATGCAGCTTGTAGTATAATTTTCAAGGTTCATCACTCTATAGCCGATGGCTTCTGTCTTATGAACACTCTTTTATCTTGCCTTAAGAGAGCCGACGATCCTTCTCTTCCTCTCACGTTCCCGTCTCGAAAACACTCAAAACAACCCAAAAACGATGCTCGTTTTGGGCTCCTTAGCCATCTCCCTCGTCGAATCTCCTCCGTCGTATCAAACTTTGGATGGAGTGTGATGAAAAACACTTTTGTTGAAGACGACCCAACACCCATCAAGCCAGAAGGAGATTGCATGCAGCTTGTCAAGCCAATTGCCATATCCACCATGACCTTTTCCCTTGATCAAATCAAACAAATCAAGAACAACCTTAACGTGGTATATATATTACTTAATTATTTACTTATAAGGTCGTGATATATTTTGATATCTTTTTCTAATAATCAATATAGGATCAAGATTAAAAATATACCATCGAGATATATATATTTTGATTTGACAGTTTGATATGATCAACCAATACTTGTGTCATGTAAATATCAATAAAACATACAAAAAAAAAGTATCAATAAAAAGTTACCAATAGAAATACTAACAATTATAAGATGGTTAAATTTCAAATTTAATTGGGTACGTATGGGTTGAGCTTAATTTTAGTCTACATAGTCTTCAGAGTTTCAATTTGATTTCTATGCATGAATCATCATTAAATTATCTCTTATCCACTTGAAAAACCAAATAATTTTTTCTTTATTGAAAACATTAACTTATAGTCATATGAAAATAAAATAAAAAATGTCACTTATTTAATATGGTAACAATAAAGCCAAATCGAGTTTAGCTCAAGTGGTTAAGACATCTATCTTTGACTAAAAGGTCACAAATTCAAACCTCCCCCCACAATGTTGTACTAAAACAAATGGTAGCAATAAAAAGATAATTTTTAAGGTTTGATTAAATTATATGAACTGCAAATTGGTAGATTCATGACTTATTTTCATAAGATTGATGAAACTTGTTCTAGCTATAGATCTCCATTCACATCGATATTTTAACCCTGGTGTGGAATTTTGGTTTTTTAACAGAGTATTAATGATGTCCTCACCGGAATCATATTCATCGGAGTTCGACTATACATGCAAGAACACAACCCTCAGTCAAGTGAGAAAAATTCAAGTGCATTGATTCTCCTCAATACTAGGAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGCTCGAAACCGACACCGATGCGCCGTGGGGAAACCGAATCGCCTTCTTGCCAGTCCCAATACCTAAACTAATTGATTCTACCCTTTCAAATAACCCTTTGGAATTTGTCAAGGAAGCCAAAGAAAAAATCATGTTGCAGAGAAACACTCTCTCCGTTTTCATGGCTGCCAAAGTTTTTGATATAGTAAACCACGTGACAGGCCCTGAGGTGAATATTTAACTTAACCCATTTCAAATTTCTTATAATATCTCCAGCTGATTAAACATGGTAATTCAATTTAATTATGATGATATCAATAATATAATGCTAATTAACTGTGTGCTAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTCAAGAATTCAAGCATCATGATATCAAGTATGATTGGGCCTTTGGAGAAAATGGCTTTGGCCAATCGTCCTGTTAAAGGCTTATACTTCACTGTCCCAGGCATGCCTCAGGTATCCAATCACAACTATTTTCTCACCAAGCTTAAGAGGGTGTAAGGGCAAGAATTTGTATTTCCACGTTTTCATCAAACAAACTCATGGGTTTCAACAGTATCCTAAACCTACTCCCTTCAAATCCTCTCAAGATCATGGGATCGGATACTCGTTTGAACTTTTGGGTTCTGATACCATATTAATATTCATCGAGTTCCTTCTAGAAGATGAGTAGTCCATGTCTATTATAAACTATTGTTAGGTCTCTTATTTTTTCAATGTGAGATACTTAACCGTGTGTGTATATATTTAACGTGTATCTGATTTATTTGTTTGTAGAGTCTACTTATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTATTTGGAGGTGAGAAGTGCTTCATAGATCAACAGAAATTGAAA

mRNA sequence

GTCAATTTTCAGATGATTTTGCGACTATAAAATGGGAAGATTCAGTAGCTCACAAACCATCAAGATCAATCCTTCCTTGTTCTAACTTCCAAAGAACCTCCCTCATTTGCACTGAATCAACACACATCTTTCATTAATGGAGATTCTTGAAGATTCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGAGTCCTCGAACTCGAATCTCCGATCTCTCCCCCTGACGCCATTTCAATTGTAACTGATGTTCTCCTCCCTGCCAACCCACGCTTTACTTCAATCATGGTAAAAGAGAAGTCAACGGGAGAAAGAAAGTGGAAGACAGCTGAAGTTAACCCAAGAGACCACATTTACGTACCGACTTTTCCAAACGACCTCTCGCCTTATGAATACGATGCTTACTTCGACGAATATGCCACGAAAACAGCCACTAAACCCTTCTCCCAAAGCATTCCTTTGTGGGAAATTCACGTTTTCAACTACCCTACAAGCCATGCAGCTTGTAGTATAATTTTCAAGGTTCATCACTCTATAGCCGATGGCTTCTGTCTTATGAACACTCTTTTATCTTGCCTTAAGAGAGCCGACGATCCTTCTCTTCCTCTCACGTTCCCGTCTCGAAAACACTCAAAACAACCCAAAAACGATGCTCGTTTTGGGCTCCTTAGCCATCTCCCTCGTCGAATCTCCTCCGTCGTATCAAACTTTGGATGGAGTGTGATGAAAAACACTTTTGTTGAAGACGACCCAACACCCATCAAGCCAGAAGGAGATTGCATGCAGCTTAGTATTAATGATGTCCTCACCGGAATCATATTCATCGGAGTTCGACTATACATGCAAGAACACAACCCTCAGTCAAGTGAGAAAAATTCAAGTGCATTGATTCTCCTCAATACTAGGAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGCTCGAAACCGACACCGATGCGCCGTGGGGAAACCGAATCGCCTTCTTGCCAGTCCCAATACCTAAACTAATTGATTCTACCCTTTCAAATAACCCTTTGGAATTTGTCAAGGAAGCCAAAGAAAAAATCATGTTGCAGAGAAACACTCTCTCCGTTTTCATGGCTGCCAAAGTTTTTGATATAGTAAACCACGTGACAGGCCCTGAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTCAAGAATTCAAGCATCATGATATCAAGTATGATTGGGCCTTTGGAGAAAATGGCTTTGGCCAATCGTCCTGTTAAAGGCTTATACTTCACTGTCCCAGGCATGCCTCAGAGTCTACTTATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTATTTGGAGGTGAGAAGTGCTTCATAGATCAACAGAAATTGAAA

Coding sequence (CDS)

ATGGAGATTCTTGAAGATTCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGAGTCCTCGAACTCGAATCTCCGATCTCTCCCCCTGACGCCATTTCAATTGTAACTGATGTTCTCCTCCCTGCCAACCCACGCTTTACTTCAATCATGGTAAAAGAGAAGTCAACGGGAGAAAGAAAGTGGAAGACAGCTGAAGTTAACCCAAGAGACCACATTTACGTACCGACTTTTCCAAACGACCTCTCGCCTTATGAATACGATGCTTACTTCGACGAATATGCCACGAAAACAGCCACTAAACCCTTCTCCCAAAGCATTCCTTTGTGGGAAATTCACGTTTTCAACTACCCTACAAGCCATGCAGCTTGTAGTATAATTTTCAAGGTTCATCACTCTATAGCCGATGGCTTCTGTCTTATGAACACTCTTTTATCTTGCCTTAAGAGAGCCGACGATCCTTCTCTTCCTCTCACGTTCCCGTCTCGAAAACACTCAAAACAACCCAAAAACGATGCTCGTTTTGGGCTCCTTAGCCATCTCCCTCGTCGAATCTCCTCCGTCGTATCAAACTTTGGATGGAGTGTGATGAAAAACACTTTTGTTGAAGACGACCCAACACCCATCAAGCCAGAAGGAGATTGCATGCAGCTTAGTATTAATGATGTCCTCACCGGAATCATATTCATCGGAGTTCGACTATACATGCAAGAACACAACCCTCAGTCAAGTGAGAAAAATTCAAGTGCATTGATTCTCCTCAATACTAGGAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGCTCGAAACCGACACCGATGCGCCGTGGGGAAACCGAATCGCCTTCTTGCCAGTCCCAATACCTAAACTAATTGATTCTACCCTTTCAAATAACCCTTTGGAATTTGTCAAGGAAGCCAAAGAAAAAATCATGTTGCAGAGAAACACTCTCTCCGTTTTCATGGCTGCCAAAGTTTTTGATATAGTAAACCACGTGACAGGCCCTGAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTCAAGAATTCAAGCATCATGATATCAAGTATGATTGGGCCTTTGGAGAAAATGGCTTTGGCCAATCGTCCTGTTAAAGGCTTATACTTCACTGTCCCAGGCATGCCTCAGAGTCTACTTATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTATTTGGAGGTGAGAAGTGCTTCATAGATCAACAGAAATTGAAA

Protein sequence

MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPKNDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLK

Homology

[Close]

BLAST of MC04g0132 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.6e-36
Identity = 114/464 (24.57%), Postives = 209/464 (45.04%), Query Frame = 0


Query: 4   LEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVK 63
           +E+   P+SP ++ F+  E    ++ V+ L++ I P   I  +   L+  +PRF+S +V 
Sbjct: 21  VEEEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVN 80

Query: 64  --EKSTGERKWKTAEVNPRDHIYVPTFPND-LSPYEYDAYFDEYATKTATKPFSQSIPLW 123
               +  E+KW    V   DH+ +P      +     D + + Y +   T P   S PLW
Sbjct: 81  NCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLW 140

Query: 124 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 183
           E+H+ +  TS A    + ++HHS+ DG  +M+ +L+C ++  +P+     PS  +  +P 
Sbjct: 141 EVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPN---ELPSLPYQNRPS 200

Query: 184 NDARFGLLSHLPRRISSVVSNFGWSVM---------KNT------------FVEDDPTPI 243
           +       S   +  S   S F W VM          NT            F++D  TPI
Sbjct: 201 SG------SSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPI 260

Query: 244 KPE------------------------GDCMQLSINDVLTGIIFIGVRLYMQEH--NPQS 303
           K +                         + M++++NDV+ G+   G+  Y++      + 
Sbjct: 261 KGDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEE 320

Query: 304 SEKNSS---------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS 363
           S++NSS         A +L+N R     + + +M+   +   WGN I ++  P       
Sbjct: 321 SKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP----FSI 380

Query: 364 TLSNNPLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMI 409
            L ++PL+ ++ AK  I  ++N+L   +   V  I+ +  G +  A +  R L N+++  
Sbjct: 381 ALCDDPLKHLRRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSF 440

[Close]

BLAST of MC04g0132 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.0e-34
Identity = 105/453 (23.18%), Postives = 208/453 (45.92%), Query Frame = 0


Query: 5   EDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKE 64
           ++   P+SP ++ F+  E   +++ V+  +S + P   I    +  +  +PRF+S +V +
Sbjct: 14  KEEEQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTD 73

Query: 65  KSTGERKWKTAEVNPRDHIYVPTF-PNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIH 124
           ++   ++W    V   DH  VP   P ++     +A+ ++Y +     P   S PLWE+H
Sbjct: 74  ENGQNQRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELH 133

Query: 125 VFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADD----PSLPLTFPSRKHSK-- 184
           + +  TS A    + K+HHS+ DG  +M+ +L+C+++  +    PSLP  + S   S   
Sbjct: 134 LLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLL 193

Query: 185 --QPKNDARFGLL-----SHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-------- 244
               ++D+R   L     + +   +++V     + ++   FV+D  TPIK +        
Sbjct: 194 TTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEF-IVTTLFVKDTETPIKGDFLSTKSKQ 253

Query: 245 ----------------GDCMQLSINDVLTGIIFIGVRLYMQEHNPQSSEKNSS------- 304
                            + M ++INDV+ G+   G+  Y+     +   KN         
Sbjct: 254 LRLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKR 313

Query: 305 ----ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVK 364
               + +L+N R     + + +M+E  +   WGN   ++  P        L ++PLE ++
Sbjct: 314 IRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP----FSIALRDDPLEHLE 373

Query: 365 EAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMA 409
            A++ I  ++N+    +      I+  + G +V A +  R + N+++  S+M+GP+E+++
Sbjct: 374 IAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVS 433

[Close]

BLAST of MC04g0132 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.4e-34
Identity = 113/439 (25.74%), Postives = 203/439 (46.24%), Query Frame = 0


Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP S+ FN+ +   +++  +  +     P  I  +   L+  +PRF+SI+  E + G+
Sbjct: 16  PLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGLEHTLV-NHPRFSSIL--EMNNGK 75

Query: 70  R-KWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
           + +W   +V   +H+ VP    D+     D Y ++Y +K  T P   S PLWE+H+    
Sbjct: 76  KPRWVRTKVKVEEHVIVPDVDPDIE--NPDQYLEDYISKLTTIPMDLSKPLWEMHLLGVK 135

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKHSKQPKNDARFGL 189
           TS+A    I K+HHS+ DG  LM+ LL+C ++  DP +LP     +K      N   F  
Sbjct: 136 TSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFNK 195

Query: 190 LSHLPRRISSVVSNFGWSVMKNTFVE------------DDPTPI--KPEGD--------- 249
           +  L      V   F   ++ NTFV+            D  TP+  KP  +         
Sbjct: 196 IWWL-----FVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHR 255

Query: 250 ------------CMQLSINDVLTGIIFIGV-----RLYMQEHNPQSSEK----NSSALIL 309
                        M++++NDVL G+   G+     R Y QE  P+S E        + I+
Sbjct: 256 IISFDDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIM 315

Query: 310 LNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIML 369
           +N R     +++ +M+   +   WGN   ++ +P        L  +PLE+V++AK  I  
Sbjct: 316 INLRPNAGIEALADMMAKKSKCRWGNLFGYILLP----FSVGLETDPLEYVRQAKATIDR 375

Query: 370 QRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKG 403
           ++++L    +   F ++  V G +    L ++ + ++++  S+++GP E++     P+  
Sbjct: 376 KKHSLEAVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNY 435

[Close]

BLAST of MC04g0132 vs. ExPASy Swiss-Prot
Match: F4IU14 (Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=WSD3 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.7e-33
Identity = 112/434 (25.81%), Postives = 202/434 (46.54%), Query Frame = 0


Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP+SQ F +  +   ++  L  ++  +P   +  V +  +   PRF+S  V+ K  G+
Sbjct: 22  PLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKL-PRFSS-KVEIKKNGK 81

Query: 70  RKWKTAEVNPRDHIYVPTFPNDLSPYEY-DAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
             W    V   DH+ VP    D S  E  D + ++Y +K A  P   S PLWE+H+ N  
Sbjct: 82  ASWVPVSVRVEDHVVVPDL--DYSNIENPDQFIEDYTSKLANTPMDMSRPLWELHLLNIK 141

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKH-SKQPKNDARFG 189
           TS+A    I K HHS+ DG  L++ LL+  ++  DP +LP T  +RKH S   K+    G
Sbjct: 142 TSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSWWLVG 201

Query: 190 LLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-GDC-------------------- 249
               + R I + V      ++   F+ D  TP+  + GD                     
Sbjct: 202 RFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVIHRIVSFDDVKLVK 261

Query: 250 --MQLSINDVLTGIIFIGVRLYM----QEHNPQSSEKNSSAL-----ILLNTRKAKAYKS 309
             M + +NDVL G+   G+  Y+     E      +KN   +     + +N R     + 
Sbjct: 262 NNMDMKVNDVLLGMTQAGLSRYLSRKYDEDMVVEKKKNLEKIRLRGTVFVNLRADTKLED 321

Query: 310 VKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAA 369
           +  M+   +   WGN +  +  P+   + S   ++PLE+V+ AK  + +++ ++   +  
Sbjct: 322 LANMMAKGSKCRWGNFVGVIIFPL--WVRS--EDDPLEYVRRAKSTMDIKKLSIESLICY 381

Query: 370 KVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQS 409
            +  +   + G +V   L +R   ++++  S+++GP E ++  + P+  +  +  G PQ+
Sbjct: 382 GLIKLTRKILGGKVVETLVRRLFDHTTLTFSNVMGPDEDISFFDHPMSYVAASALGGPQA 441

[Close]

BLAST of MC04g0132 vs. ExPASy Swiss-Prot
Match: Q9M3B1 (Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=WSD6 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 8.3e-33
Identity = 110/459 (23.97%), Postives = 203/459 (44.23%), Query Frame = 0


Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP ++ F+  E    V+ V+ ++  I P   I  +   L+  +PRF+S MV   S G 
Sbjct: 27  PLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQTLI-RHPRFSSKMV-STSVGN 86

Query: 70  RK-----WKTAEVNPRDHIYVPTF-PNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIH 129
           +K     W    V   DH+ V      ++     DA+ + Y +   T P   S PLW++H
Sbjct: 87  KKRQTQSWVRTNVVVTDHVIVSDIQTQNIENGNADAFLETYVSNLTTVPLDISKPLWQLH 146

Query: 130 VFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQ----- 189
           + +  TS A    + K HHS+ DG  LM  +L+C+++  +P    + P++  S       
Sbjct: 147 LLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSSSRSSRL 206

Query: 190 ---PKNDARFGLLSHLPRRISSVVSNFGWSVMK----NTFVEDDPTPIKPE--------- 249
               + D+RF  L  +      +V N     ++      F++D  TPIK +         
Sbjct: 207 MAGSRGDSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIKGDFRFSKSKRM 266

Query: 250 ---------------GDCMQLSINDVLTGIIFIGVRLYMQEH---------NPQSSEKNS 309
                           + M++++NDV+ G+   G+  Y+              Q S++ +
Sbjct: 267 CLVHRTVSLDDIKLIKNTMKMTVNDVVLGVSQAGLSQYLDRRYGEKKKKVGEDQDSKRKA 326

Query: 310 S---------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNN 369
           +         + +L+N R     + + +M+   +   WGN I ++  P        L ++
Sbjct: 327 TDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFP----FSIGLRDD 386

Query: 370 PLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIG 409
           PL+ ++ AK  I  ++N+L   +       +    G +V AK+  R L N+++  S++IG
Sbjct: 387 PLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVAAKIINRALSNTTMSFSNLIG 446

[Close]

BLAST of MC04g0132 vs. NCBI nr
Match: XP_022147882.1 (O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransferase WSD1-like [Momordica charantia])

HSP 1 Score: 835 bits (2158), Expect = 1.28e-304
Identity = 423/448 (94.42%), Postives = 423/448 (94.42%), Query Frame = 0


Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI
Sbjct: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW
Sbjct: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK
Sbjct: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180

Query: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL------------ 240
           NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL            
Sbjct: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240

Query: 241 -------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300
                        SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV
Sbjct: 241 LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300

Query: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360
           KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK
Sbjct: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360

Query: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420
           VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL
Sbjct: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420

Query: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           LITIVSYMGDLRIVFGGEKCFIDQQKLK
Sbjct: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLK 448

[Close]

BLAST of MC04g0132 vs. NCBI nr
Match: XP_011659709.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_017048 [Cucumis sativus])

HSP 1 Score: 688 bits (1775), Expect = 3.32e-246
Identity = 350/454 (77.09%), Postives = 388/454 (85.46%), Query Frame = 0


Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISP-PDAISIVTDVLLPANPRFTS 60
           MEI EDST PMSPLSQYFNT EMCVSVLGV+ELE+PIS   D  SIVTDVL+PANPRFTS
Sbjct: 1   MEIYEDSTAPMSPLSQYFNTCEMCVSVLGVIELETPISSWEDISSIVTDVLVPANPRFTS 60

Query: 61  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 120
           IMVKEK T ERKWKT +VNP+DHIY+P FP +LS YEYDAYFDEYATKTATKPFSQ+IPL
Sbjct: 61  IMVKEK-TEERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAIPL 120

Query: 121 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 180
           WEIHVFNYPT+HA CSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 121 WEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 180

Query: 181 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL------- 240
            N+ +F  LSH P R    IS+ V NFGWS+MKNTFVEDDPTPIKP+ D MQL       
Sbjct: 181 GNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIAIS 240

Query: 241 ------------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 300
                             S+NDVLTGIIF+G+RLYMQEHNP+SS  NSSALILLNTRKAK
Sbjct: 241 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRKAK 300

Query: 301 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 360
           AYKSVKEM++ D+DAPWGN+IAFLP+PIPKLIDS  +S+ PLEFV++ KEKIMLQR+ LS
Sbjct: 301 AYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSPLS 360

Query: 361 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 420
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS+MIGP+EKMAL N PVKGLYFTVP
Sbjct: 361 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNMIGPVEKMALVNLPVKGLYFTVP 420

Query: 421 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLK
Sbjct: 421 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLK 453

[Close]

BLAST of MC04g0132 vs. NCBI nr
Match: XP_022952997.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 662 bits (1707), Expect = 1.23e-235
Identity = 338/452 (74.78%), Postives = 372/452 (82.30%), Query Frame = 0


Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDS  PMSPLSQYFNTS MCVSVLG +ELE+PIS   A++I T VL+PANPRF+SI
Sbjct: 1   MEILEDSAAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFSSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEK  GERKWK  EVN  DHIY P FP DLSPYEYDAYFDEYAT TATKPFSQSIPLW
Sbjct: 61  MVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPT+HA C++IFKVHHSIADGFCLMNTLLS LKRADDPSLPLTFPSRK SKQP+
Sbjct: 121 EIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQPE 180

Query: 181 NDARFGLLSHLPR----RISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL-------- 240
           N   FG LSH P      + + +SNFG+S+  NTFVEDDPTPIK EGD MQL        
Sbjct: 181 NAHDFGRLSHFPACLFLSLRNFLSNFGYSIA-NTFVEDDPTPIKHEGDSMQLVKPIAIST 240

Query: 241 -----------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKA 300
                             +NDV+TGIIF+G+RLYMQEH+P S E NSSALILLNTRKAKA
Sbjct: 241 MTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKAKA 300

Query: 301 YKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVF 360
           YKSV+EM++TD+D PWGNRIAFLPVPIPKL+DSTLS  PLEFVKEAK+ IMLQR+ LSVF
Sbjct: 301 YKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLSVF 360

Query: 361 MAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGM 420
           MAAKVFD++ HVTGPE+GAKLFKRKL NSS +IS+MIGP EKMAL NRPVKGLYFTVPG+
Sbjct: 361 MAAKVFDMLKHVTGPEMGAKLFKRKLNNSSTIISNMIGPTEKMALVNRPVKGLYFTVPGV 420

Query: 421 PQSLLITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           PQSL+ITIVSYMGDLRIVFGGEKCFID  KLK
Sbjct: 421 PQSLIITIVSYMGDLRIVFGGEKCFIDLHKLK 451

[Close]

BLAST of MC04g0132 vs. NCBI nr
Match: XP_038887587.1 (O-acyltransferase WSD1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 623 bits (1607), Expect = 3.78e-221
Identity = 313/425 (73.65%), Postives = 358/425 (84.24%), Query Frame = 0


Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAIS-IVTDVLLPANPRFTS 60
           ME  E+ST PMSP+SQYFNTSEMCVSVLGV+ELE+PIS  + IS I  DVL+P+NPRFTS
Sbjct: 1   MENYEESTAPMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRFTS 60

Query: 61  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 120
           +MVKE S GERKWKT ++NP+DHIY+P FP +LSPYEYDAYFDEYAT+TATKPFSQSIPL
Sbjct: 61  VMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSIPL 120

Query: 121 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 180
           WEIHVFNYPTSHA CSIIFKVHHSIADGFCLMN LLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 121 WEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSKQP 180

Query: 181 KNDARFGLLSHLPRRISSVVSNF----GWSVMKNTFVEDDPTPIKPEGDCMQL------- 240
           +N+  F   S+ P  + S VSNF    GWS+MKNT VEDDPTPIKPE D MQL       
Sbjct: 181 ENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIAIS 240

Query: 241 ------------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 300
                             S+NDV+TGIIF+G+RLYMQEHNP+SS+ NSSALILLNTRKAK
Sbjct: 241 TITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRKAK 300

Query: 301 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDST-LSNNPLEFVKEAKEKIMLQRNTLS 360
           AYKSVKEM++T+TDAPWGN+IAFLPVPIPKLIDST L++ PLEFVK+AKEKIMLQR+ +S
Sbjct: 301 AYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSPVS 360

Query: 361 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 394
           VF+AAK F++V +VTGPEVGAKLFKRKLKNSSIMIS+MIGP+EK++L N PVKG+YFTVP
Sbjct: 361 VFLAAKFFEMVKNVTGPEVGAKLFKRKLKNSSIMISNMIGPVEKISLVNHPVKGIYFTVP 420

[Close]

BLAST of MC04g0132 vs. NCBI nr
Match: XP_038887586.1 (O-acyltransferase WSD1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 623 bits (1606), Expect = 1.19e-220
Identity = 313/424 (73.82%), Postives = 357/424 (84.20%), Query Frame = 0


Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAIS-IVTDVLLPANPRFTS 60
           ME  E+ST PMSP+SQYFNTSEMCVSVLGV+ELE+PIS  + IS I  DVL+P+NPRFTS
Sbjct: 1   MENYEESTAPMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRFTS 60

Query: 61  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 120
           +MVKE S GERKWKT ++NP+DHIY+P FP +LSPYEYDAYFDEYAT+TATKPFSQSIPL
Sbjct: 61  VMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSIPL 120

Query: 121 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 180
           WEIHVFNYPTSHA CSIIFKVHHSIADGFCLMN LLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 121 WEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSKQP 180

Query: 181 KNDARFGLLSHLPRRISSVVSNF----GWSVMKNTFVEDDPTPIKPEGDCMQL------- 240
           +N+  F   S+ P  + S VSNF    GWS+MKNT VEDDPTPIKPE D MQL       
Sbjct: 181 ENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIAIS 240

Query: 241 ------------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 300
                             S+NDV+TGIIF+G+RLYMQEHNP+SS+ NSSALILLNTRKAK
Sbjct: 241 TITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRKAK 300

Query: 301 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDST-LSNNPLEFVKEAKEKIMLQRNTLS 360
           AYKSVKEM++T+TDAPWGN+IAFLPVPIPKLIDST L++ PLEFVK+AKEKIMLQR+ +S
Sbjct: 301 AYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSPVS 360

Query: 361 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 393
           VF+AAK F++V +VTGPEVGAKLFKRKLKNSSIMIS+MIGP+EK++L N PVKG+YFTVP
Sbjct: 361 VFLAAKFFEMVKNVTGPEVGAKLFKRKLKNSSIMISNMIGPVEKISLVNHPVKGIYFTVP 420

[Close]

BLAST of MC04g0132 vs. ExPASy TrEMBL
Match: A0A6J1D2I7 (Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 PE=4 SV=1)

HSP 1 Score: 835 bits (2158), Expect = 6.21e-305
Identity = 423/448 (94.42%), Postives = 423/448 (94.42%), Query Frame = 0


Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI
Sbjct: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW
Sbjct: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK
Sbjct: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180

Query: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL------------ 240
           NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL            
Sbjct: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240

Query: 241 -------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300
                        SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV
Sbjct: 241 LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300

Query: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360
           KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK
Sbjct: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360

Query: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420
           VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL
Sbjct: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420

Query: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           LITIVSYMGDLRIVFGGEKCFIDQQKLK
Sbjct: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLK 448

[Close]

BLAST of MC04g0132 vs. ExPASy TrEMBL
Match: A0A6J1GLZ3 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 PE=4 SV=1)

HSP 1 Score: 662 bits (1707), Expect = 5.96e-236
Identity = 338/452 (74.78%), Postives = 372/452 (82.30%), Query Frame = 0


Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDS  PMSPLSQYFNTS MCVSVLG +ELE+PIS   A++I T VL+PANPRF+SI
Sbjct: 1   MEILEDSAAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFSSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEK  GERKWK  EVN  DHIY P FP DLSPYEYDAYFDEYAT TATKPFSQSIPLW
Sbjct: 61  MVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPT+HA C++IFKVHHSIADGFCLMNTLLS LKRADDPSLPLTFPSRK SKQP+
Sbjct: 121 EIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQPE 180

Query: 181 NDARFGLLSHLPR----RISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL-------- 240
           N   FG LSH P      + + +SNFG+S+  NTFVEDDPTPIK EGD MQL        
Sbjct: 181 NAHDFGRLSHFPACLFLSLRNFLSNFGYSIA-NTFVEDDPTPIKHEGDSMQLVKPIAIST 240

Query: 241 -----------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKA 300
                             +NDV+TGIIF+G+RLYMQEH+P S E NSSALILLNTRKAKA
Sbjct: 241 MTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKAKA 300

Query: 301 YKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVF 360
           YKSV+EM++TD+D PWGNRIAFLPVPIPKL+DSTLS  PLEFVKEAK+ IMLQR+ LSVF
Sbjct: 301 YKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLSVF 360

Query: 361 MAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGM 420
           MAAKVFD++ HVTGPE+GAKLFKRKL NSS +IS+MIGP EKMAL NRPVKGLYFTVPG+
Sbjct: 361 MAAKVFDMLKHVTGPEMGAKLFKRKLNNSSTIISNMIGPTEKMALVNRPVKGLYFTVPGV 420

Query: 421 PQSLLITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           PQSL+ITIVSYMGDLRIVFGGEKCFID  KLK
Sbjct: 421 PQSLIITIVSYMGDLRIVFGGEKCFIDLHKLK 451

[Close]

BLAST of MC04g0132 vs. ExPASy TrEMBL
Match: A0A0A0K2E9 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 1.03e-211
Identity = 299/394 (75.89%), Postives = 335/394 (85.03%), Query Frame = 0


Query: 60  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 119
           + VKEK T ERKWKT +VNP+DHIY+P FP +LS YEYDAYFDEYATKTATKPFSQ+IPL
Sbjct: 1   MQVKEK-TEERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 120 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 179
           WEIHVFNYPT+HA CSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 61  WEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 180 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL------- 239
            N+ +F  LSH P R    IS+ V NFGWS+MKNTFVEDDPTPIKP+ D MQL       
Sbjct: 121 GNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIAIS 180

Query: 240 ------------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 299
                             S+NDVLTGIIF+G+RLYMQEHNP+SS  NSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRKAK 240

Query: 300 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 359
           AYKSVKEM++ D+DAPWGN+IAFLP+PIPKLIDS  +S+ PLEFV++ KEKIMLQR+ LS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSPLS 300

Query: 360 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 419
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS+MIGP+EKMAL N PVKGLYFTVP
Sbjct: 301 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNMIGPVEKMALVNLPVKGLYFTVP 360

Query: 420 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLK
Sbjct: 361 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLK 393

[Close]

BLAST of MC04g0132 vs. ExPASy TrEMBL
Match: A0A5D3C6U2 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002990 PE=4 SV=1)

HSP 1 Score: 591 bits (1523), Expect = 3.69e-209
Identity = 297/394 (75.38%), Postives = 333/394 (84.52%), Query Frame = 0


Query: 60  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 119
           + VKEK T ERKWKT +V+ +DHI +P FP +LS YEYDAYFDEYATKTATKPFSQ+IPL
Sbjct: 1   MQVKEK-TEERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 120 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 179
           WEIHVFNYPT+HA CS+IFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 180 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL------- 239
           KN   F  LSH P R    +S+ V NFGWS+MKNTFVEDDPTPIKP+ D MQL       
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 240 ------------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 299
                             S+NDVLTGIIF+GVRLYMQEHNP+SSE NSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 300 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 359
           AYKSVKEM++ D+DAPWGN+IAFLPVPIPKLIDS  +S+ PLEFV++ KEKI LQR+ LS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 360 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 419
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS++IGP+EKMAL NRPVKGLYFTVP
Sbjct: 301 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNLIGPVEKMALVNRPVKGLYFTVP 360

Query: 420 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLK
Sbjct: 361 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLK 393

[Close]

BLAST of MC04g0132 vs. ExPASy TrEMBL
Match: A0A5A7SPR9 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G002950 PE=4 SV=1)

HSP 1 Score: 590 bits (1520), Expect = 1.06e-208
Identity = 296/394 (75.13%), Postives = 333/394 (84.52%), Query Frame = 0


Query: 60  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 119
           + VKEK T ERKWKT +V+ +DHI +P FP +LS YEYDAYFDEY+TKTATKPFSQ+IPL
Sbjct: 1   MQVKEK-TEERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYSTKTATKPFSQAIPL 60

Query: 120 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 179
           WEIHVFNYPT+HA CS+IFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 180 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL------- 239
           KN   F  LSH P R    +S+ V NFGWS+MKNTFVEDDPTPIKP+ D MQL       
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 240 ------------------SINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 299
                             S+NDVLTGIIF+GVRLYMQEHNP+SSE NSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 300 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 359
           AYKSVKEM++ D+DAPWGN+IAFLPVPIPKLIDS  +S+ PLEFV++ KEKI LQR+ LS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 360 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 419
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS++IGP+EKMAL NRPVKGLYFTVP
Sbjct: 301 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNLIGPVEKMALVNRPVKGLYFTVP 360

Query: 420 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLK 423
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLK
Sbjct: 361 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLK 393

[Close]

BLAST of MC04g0132 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-37
Identity = 114/464 (24.57%), Postives = 209/464 (45.04%), Query Frame = 0


Query: 4   LEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVK 63
           +E+   P+SP ++ F+  E    ++ V+ L++ I P   I  +   L+  +PRF+S +V 
Sbjct: 21  VEEEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVN 80

Query: 64  --EKSTGERKWKTAEVNPRDHIYVPTFPND-LSPYEYDAYFDEYATKTATKPFSQSIPLW 123
               +  E+KW    V   DH+ +P      +     D + + Y +   T P   S PLW
Sbjct: 81  NCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLW 140

Query: 124 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 183
           E+H+ +  TS A    + ++HHS+ DG  +M+ +L+C ++  +P+     PS  +  +P 
Sbjct: 141 EVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPN---ELPSLPYQNRPS 200

Query: 184 NDARFGLLSHLPRRISSVVSNFGWSVM---------KNT------------FVEDDPTPI 243
           +       S   +  S   S F W VM          NT            F++D  TPI
Sbjct: 201 SG------SSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPI 260

Query: 244 KPE------------------------GDCMQLSINDVLTGIIFIGVRLYMQEH--NPQS 303
           K +                         + M++++NDV+ G+   G+  Y++      + 
Sbjct: 261 KGDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEE 320

Query: 304 SEKNSS---------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS 363
           S++NSS         A +L+N R     + + +M+   +   WGN I ++  P       
Sbjct: 321 SKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP----FSI 380

Query: 364 TLSNNPLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMI 409
            L ++PL+ ++ AK  I  ++N+L   +   V  I+ +  G +  A +  R L N+++  
Sbjct: 381 ALCDDPLKHLRRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSF 440

[Close]

BLAST of MC04g0132 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-35
Identity = 105/453 (23.18%), Postives = 208/453 (45.92%), Query Frame = 0


Query: 5   EDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKE 64
           ++   P+SP ++ F+  E   +++ V+  +S + P   I    +  +  +PRF+S +V +
Sbjct: 14  KEEEQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTD 73

Query: 65  KSTGERKWKTAEVNPRDHIYVPTF-PNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIH 124
           ++   ++W    V   DH  VP   P ++     +A+ ++Y +     P   S PLWE+H
Sbjct: 74  ENGQNQRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELH 133

Query: 125 VFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADD----PSLPLTFPSRKHSK-- 184
           + +  TS A    + K+HHS+ DG  +M+ +L+C+++  +    PSLP  + S   S   
Sbjct: 134 LLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLL 193

Query: 185 --QPKNDARFGLL-----SHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-------- 244
               ++D+R   L     + +   +++V     + ++   FV+D  TPIK +        
Sbjct: 194 TTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEF-IVTTLFVKDTETPIKGDFLSTKSKQ 253

Query: 245 ----------------GDCMQLSINDVLTGIIFIGVRLYMQEHNPQSSEKNSS------- 304
                            + M ++INDV+ G+   G+  Y+     +   KN         
Sbjct: 254 LRLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKR 313

Query: 305 ----ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVK 364
               + +L+N R     + + +M+E  +   WGN   ++  P        L ++PLE ++
Sbjct: 314 IRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP----FSIALRDDPLEHLE 373

Query: 365 EAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMA 409
            A++ I  ++N+    +      I+  + G +V A +  R + N+++  S+M+GP+E+++
Sbjct: 374 IAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVS 433

[Close]

BLAST of MC04g0132 vs. TAIR 10
Match: AT5G53390.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 147.5 bits (371), Expect = 2.4e-35
Identity = 113/439 (25.74%), Postives = 203/439 (46.24%), Query Frame = 0


Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP S+ FN+ +   +++  +  +     P  I  +   L+  +PRF+SI+  E + G+
Sbjct: 16  PLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGLEHTLV-NHPRFSSIL--EMNNGK 75

Query: 70  R-KWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
           + +W   +V   +H+ VP    D+     D Y ++Y +K  T P   S PLWE+H+    
Sbjct: 76  KPRWVRTKVKVEEHVIVPDVDPDIE--NPDQYLEDYISKLTTIPMDLSKPLWEMHLLGVK 135

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKHSKQPKNDARFGL 189
           TS+A    I K+HHS+ DG  LM+ LL+C ++  DP +LP     +K      N   F  
Sbjct: 136 TSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFNK 195

Query: 190 LSHLPRRISSVVSNFGWSVMKNTFVE------------DDPTPI--KPEGD--------- 249
           +  L      V   F   ++ NTFV+            D  TP+  KP  +         
Sbjct: 196 IWWL-----FVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHR 255

Query: 250 ------------CMQLSINDVLTGIIFIGV-----RLYMQEHNPQSSEK----NSSALIL 309
                        M++++NDVL G+   G+     R Y QE  P+S E        + I+
Sbjct: 256 IISFDDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIM 315

Query: 310 LNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIML 369
           +N R     +++ +M+   +   WGN   ++ +P        L  +PLE+V++AK  I  
Sbjct: 316 INLRPNAGIEALADMMAKKSKCRWGNLFGYILLP----FSVGLETDPLEYVRQAKATIDR 375

Query: 370 QRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKG 403
           ++++L    +   F ++  V G +    L ++ + ++++  S+++GP E++     P+  
Sbjct: 376 KKHSLEAVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNY 435

[Close]

BLAST of MC04g0132 vs. TAIR 10
Match: AT2G38995.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 145.6 bits (366), Expect = 9.1e-35
Identity = 113/435 (25.98%), Postives = 203/435 (46.67%), Query Frame = 0


Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP+SQ F +  +   ++  L  ++  +P   +  V +  +   PRF+S  V+ K  G+
Sbjct: 22  PLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKL-PRFSS-KVEIKKNGK 81

Query: 70  RKWKTAEVNPRDHIYVPTFPNDLSPYEY-DAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
             W    V   DH+ VP    D S  E  D + ++Y +K A  P   S PLWE+H+ N  
Sbjct: 82  ASWVPVSVRVEDHVVVPDL--DYSNIENPDQFIEDYTSKLANTPMDMSRPLWELHLLNIK 141

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKH-SKQPKNDARFG 189
           TS+A    I K HHS+ DG  L++ LL+  ++  DP +LP T  +RKH S   K+    G
Sbjct: 142 TSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSWWLVG 201

Query: 190 LLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-GDC-------------------- 249
               + R I + V      ++   F+ D  TP+  + GD                     
Sbjct: 202 RFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVIHRIVSFDDVKLVK 261

Query: 250 --MQLSINDVLTGIIFIGV-----RLYMQEHNPQSSEKNSSAL-----ILLNTRKAKAYK 309
             M + +NDVL G+   G+     R Y+ E      +KN   +     + +N R     +
Sbjct: 262 NNMDMKVNDVLLGMTQAGLSRYLSRKYVDEDMVVEKKKNLEKIRLRGTVFVNLRADTKLE 321

Query: 310 SVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMA 369
            +  M+   +   WGN +  +  P+   + S   ++PLE+V+ AK  + +++ ++   + 
Sbjct: 322 DLANMMAKGSKCRWGNFVGVIIFPL--WVRS--EDDPLEYVRRAKSTMDIKKLSIESLIC 381

Query: 370 AKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQ 409
             +  +   + G +V   L +R   ++++  S+++GP E ++  + P+  +  +  G PQ
Sbjct: 382 YGLIKLTRKILGGKVVETLVRRLFDHTTLTFSNVMGPDEDISFFDHPMSYVAASALGGPQ 441

[Close]

BLAST of MC04g0132 vs. TAIR 10
Match: AT2G38995.2 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-34
Identity = 112/434 (25.81%), Postives = 202/434 (46.54%), Query Frame = 0


Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP+SQ F +  +   ++  L  ++  +P   +  V +  +   PRF+S  V+ K  G+
Sbjct: 22  PLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKL-PRFSS-KVEIKKNGK 81

Query: 70  RKWKTAEVNPRDHIYVPTFPNDLSPYEY-DAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
             W    V   DH+ VP    D S  E  D + ++Y +K A  P   S PLWE+H+ N  
Sbjct: 82  ASWVPVSVRVEDHVVVPDL--DYSNIENPDQFIEDYTSKLANTPMDMSRPLWELHLLNIK 141

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKH-SKQPKNDARFG 189
           TS+A    I K HHS+ DG  L++ LL+  ++  DP +LP T  +RKH S   K+    G
Sbjct: 142 TSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSWWLVG 201

Query: 190 LLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-GDC-------------------- 249
               + R I + V      ++   F+ D  TP+  + GD                     
Sbjct: 202 RFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVIHRIVSFDDVKLVK 261

Query: 250 --MQLSINDVLTGIIFIGVRLYM----QEHNPQSSEKNSSAL-----ILLNTRKAKAYKS 309
             M + +NDVL G+   G+  Y+     E      +KN   +     + +N R     + 
Sbjct: 262 NNMDMKVNDVLLGMTQAGLSRYLSRKYDEDMVVEKKKNLEKIRLRGTVFVNLRADTKLED 321

Query: 310 VKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAA 369
           +  M+   +   WGN +  +  P+   + S   ++PLE+V+ AK  + +++ ++   +  
Sbjct: 322 LANMMAKGSKCRWGNFVGVIIFPL--WVRS--EDDPLEYVRRAKSTMDIKKLSIESLICY 381

Query: 370 KVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQS 409
            +  +   + G +V   L +R   ++++  S+++GP E ++  + P+  +  +  G PQ+
Sbjct: 382 GLIKLTRKILGGKVVETLVRRLFDHTTLTFSNVMGPDEDISFFDHPMSYVAASALGGPQA 441

The following BLAST results are available for this feature:

BLAST of MC04g0132 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3B2	1.6e-36	24.57	Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3...	[more]
Q9M3B3	2.0e-34	23.18	Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3...	[more]
Q5KS41	3.4e-34	25.74	Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=...	[more]
F4IU14	3.7e-33	25.81	Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3...	[more]
Q9M3B1	8.3e-33	23.97	Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of MC04g0132 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147882.1	1.28e-304	94.42	O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransfer...	[more]
XP_011659709.2	3.32e-246	77.09	O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_01...	[more]
XP_022952997.1	1.23e-235	74.78	O-acyltransferase WSD1-like [Cucurbita moschata]	[more]
XP_038887587.1	3.78e-221	73.65	O-acyltransferase WSD1-like isoform X2 [Benincasa hispida]	[more]
XP_038887586.1	1.19e-220	73.82	O-acyltransferase WSD1-like isoform X1 [Benincasa hispida]	[more]

BLAST of MC04g0132 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2I7	6.21e-305	94.42	Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 ...	[more]
A0A6J1GLZ3	5.96e-236	74.78	Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 P...	[more]
A0A0A0K2E9	1.03e-211	75.89	Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4...	[more]
A0A5D3C6U2	3.69e-209	75.38	Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7SPR9	1.06e-208	75.13	Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of MC04g0132 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49200.1	1.2e-37	24.57	O-acyltransferase (WSD1-like) family protein	[more]
AT3G49190.1	1.4e-35	23.18	O-acyltransferase (WSD1-like) family protein	[more]
AT5G53390.1	2.4e-35	25.74	O-acyltransferase (WSD1-like) family protein	[more]
AT2G38995.1	9.1e-35	25.98	O-acyltransferase (WSD1-like) family protein	[more]
AT2G38995.2	2.7e-34	25.81	O-acyltransferase (WSD1-like) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004255	O-acyltransferase, WSD1, N-terminal	PFAM	PF03007	WES_acyltransf	coord: 52..199 e-value: 4.1E-7 score: 30.0
IPR009721	O-acyltransferase WSD1, C-terminal	PFAM	PF06974	WS_DGAT_C	coord: 287..422 e-value: 4.0E-30 score: 104.7
IPR045034	O-acyltransferase WSD1-like	PANTHER	PTHR31650	O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN	coord: 6..423
None	No IPR available	PANTHER	PTHR31650:SF34	O-ACYLTRANSFERASE WSD1-LIKE	coord: 6..423

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0132.1	MC04g0132.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019432	triglyceride biosynthetic process
biological_process	GO:0045017	glycerolipid biosynthetic process
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004144	diacylglycerol O-acyltransferase activity
molecular_function	GO:0008374	O-acyltransferase activity