MC04g0089 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0089
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	MC04: 656453 .. 657923 (-)
RNA-Seq Expression	MC04g0089
Synteny	MC04g0089

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCTCATCTTCCTCCTCTGCCTCTCCCCATTTTCAGGTAATTCTTCAAAATCCAAACTCTGCTCTGTTTTTTTCCCAGCAGAATCCTGGGTTTAATCAATAATGCGGTGTTCTTTTTGCTACAGATGGTGGTTCCATTGGAATCAACTATGGCAGAATCGCCAACAACCTCCCTTCCGCCGTTAAGGTCGTTAAGCTCCTCAAATCCCACGGCCTCCACAGGGTCAAAGTTTACGACACCGATCCGGCCGTCCTACGGGCTCTCTCCGGCTCCGGCATTAAGGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCCAAACGCCTCACCTTCGCCTACTCCTGGGTCGAGAAGAACGTCGCCGCCTACTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGAAACGAAGTCTTCGTCGACCCCCACAACACGACGTCGTTTCTTGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAGTACAACCTCCACTCCGCCATTAAAGTCTCTTCCCCCATCGCATTGAGCGCCTTGCAAAACTCCTACCCTTCCTCCGCCGGCTCCTTCCGGCCGGAGCTCGTCGAGACCGTTTTCCGGCCAATGCTGGACTTCCTCCGCCAAACCGGCTCCTACCTCATGGTAAACGCATACCCGTTCTTCGCCTACGAGTCCAACTCCGACGTCATTTCCTTAGACTACGCACTTTTCCGCGACAACCCCGGCGTGGTGGATTCCGGCAGCGGCTACCGGTACTTCAGCCTTTTCGACGCCCAAATCGACGCCGTTTTTGCCGCGATGTCGGCTCTCAAATACGACGACATTAAAATGGTGGTTACGGAGACCGGATGGCCGTCGAAGGGTGACGAGAACGAGATCGGCGCGAGCTTGGAGAACGCCGCGGCCTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGCTAAGGCCTAAAGCAGATCTGACCGTCTATTTATTTGCCCTATTCAACGAGAACAAAAAGACCGGCCCCGCCTCCGAAAGAAACTACGGCCTATTTTACCCTAACGAGGAGAAAGTTTACGACATTCCGTTCACCACCGACGGGCTGAAGGACTACCACGACAAGCCGTCGCCGGCGCCAGCAGCCGGCGGAGACGGCGGCCACATGGCGCCGGTGAGCGGCGACGGAGGCGTGTCGAAAAGTCAAACGGGGAACACGTGGTGCGTGGCGAGTGGTGAGGCCGGAAAAGAGAAACTGCAGGCGAGTCTAGATTACGCGTGTGGGGAAGGAGGGGCGGACTGCCGTCCGATCCAGCCGGATGCCACGTGTTACAATCCGAACACGCTGGAGGCCCACGCATCGTACGCCTTCAATTCTTATTATCAGAAGAACGGCCGCAAGATCGGCACGTGCTACTTCGGAGGGGCGGCGTACGTTGTCACGCAACCACCTAGTAAGTACTCTATA

mRNA sequence

TTCCTCATCTTCCTCCTCTGCCTCTCCCCATTTTCAGATGGTGGTTCCATTGGAATCAACTATGGCAGAATCGCCAACAACCTCCCTTCCGCCGTTAAGGTCGTTAAGCTCCTCAAATCCCACGGCCTCCACAGGGTCAAAGTTTACGACACCGATCCGGCCGTCCTACGGGCTCTCTCCGGCTCCGGCATTAAGGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCCAAACGCCTCACCTTCGCCTACTCCTGGGTCGAGAAGAACGTCGCCGCCTACTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGAAACGAAGTCTTCGTCGACCCCCACAACACGACGTCGTTTCTTGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAGTACAACCTCCACTCCGCCATTAAAGTCTCTTCCCCCATCGCATTGAGCGCCTTGCAAAACTCCTACCCTTCCTCCGCCGGCTCCTTCCGGCCGGAGCTCGTCGAGACCGTTTTCCGGCCAATGCTGGACTTCCTCCGCCAAACCGGCTCCTACCTCATGGTAAACGCATACCCGTTCTTCGCCTACGAGTCCAACTCCGACGTCATTTCCTTAGACTACGCACTTTTCCGCGACAACCCCGGCGTGGTGGATTCCGGCAGCGGCTACCGGTACTTCAGCCTTTTCGACGCCCAAATCGACGCCGTTTTTGCCGCGATGTCGGCTCTCAAATACGACGACATTAAAATGGTGGTTACGGAGACCGGATGGCCGTCGAAGGGTGACGAGAACGAGATCGGCGCGAGCTTGGAGAACGCCGCGGCCTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGCTAAGGCCTAAAGCAGATCTGACCGTCTATTTATTTGCCCTATTCAACGAGAACAAAAAGACCGGCCCCGCCTCCGAAAGAAACTACGGCCTATTTTACCCTAACGAGGAGAAAGTTTACGACATTCCGTTCACCACCGACGGGCTGAAGGACTACCACGACAAGCCGTCGCCGGCGCCAGCAGCCGGCGGAGACGGCGGCCACATGGCGCCGGTGAGCGGCGACGGAGGCGTGTCGAAAAGTCAAACGGGGAACACGTGGTGCGTGGCGAGTGGTGAGGCCGGAAAAGAGAAACTGCAGGCGAGTCTAGATTACGCGTGTGGGGAAGGAGGGGCGGACTGCCGTCCGATCCAGCCGGATGCCACGTGTTACAATCCGAACACGCTGGAGGCCCACGCATCGTACGCCTTCAATTCTTATTATCAGAAGAACGGCCGCAAGATCGGCACGTGCTACTTCGGAGGGGCGGCGTACGTTGTCACGCAACCACCTAGTAAGTACTCTATA

Coding sequence (CDS)

TTCCTCATCTTCCTCCTCTGCCTCTCCCCATTTTCAGATGGTGGTTCCATTGGAATCAACTATGGCAGAATCGCCAACAACCTCCCTTCCGCCGTTAAGGTCGTTAAGCTCCTCAAATCCCACGGCCTCCACAGGGTCAAAGTTTACGACACCGATCCGGCCGTCCTACGGGCTCTCTCCGGCTCCGGCATTAAGGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCCAAACGCCTCACCTTCGCCTACTCCTGGGTCGAGAAGAACGTCGCCGCCTACTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGAAACGAAGTCTTCGTCGACCCCCACAACACGACGTCGTTTCTTGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAGTACAACCTCCACTCCGCCATTAAAGTCTCTTCCCCCATCGCATTGAGCGCCTTGCAAAACTCCTACCCTTCCTCCGCCGGCTCCTTCCGGCCGGAGCTCGTCGAGACCGTTTTCCGGCCAATGCTGGACTTCCTCCGCCAAACCGGCTCCTACCTCATGGTAAACGCATACCCGTTCTTCGCCTACGAGTCCAACTCCGACGTCATTTCCTTAGACTACGCACTTTTCCGCGACAACCCCGGCGTGGTGGATTCCGGCAGCGGCTACCGGTACTTCAGCCTTTTCGACGCCCAAATCGACGCCGTTTTTGCCGCGATGTCGGCTCTCAAATACGACGACATTAAAATGGTGGTTACGGAGACCGGATGGCCGTCGAAGGGTGACGAGAACGAGATCGGCGCGAGCTTGGAGAACGCCGCGGCCTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGCTAAGGCCTAAAGCAGATCTGACCGTCTATTTATTTGCCCTATTCAACGAGAACAAAAAGACCGGCCCCGCCTCCGAAAGAAACTACGGCCTATTTTACCCTAACGAGGAGAAAGTTTACGACATTCCGTTCACCACCGACGGGCTGAAGGACTACCACGACAAGCCGTCGCCGGCGCCAGCAGCCGGCGGAGACGGCGGCCACATGGCGCCGGTGAGCGGCGACGGAGGCGTGTCGAAAAGTCAAACGGGGAACACGTGGTGCGTGGCGAGTGGTGAGGCCGGAAAAGAGAAACTGCAGGCGAGTCTAGATTACGCGTGTGGGGAAGGAGGGGCGGACTGCCGTCCGATCCAGCCGGATGCCACGTGTTACAATCCGAACACGCTGGAGGCCCACGCATCGTACGCCTTCAATTCTTATTATCAGAAGAACGGCCGCAAGATCGGCACGTGCTACTTCGGAGGGGCGGCGTACGTTGTCACGCAACCACCTAGTAAGTACTCTATA

Protein sequence

FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSKYSI

Homology

[Close]

BLAST of MC04g0089 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.5e-99
Identity = 190/464 (40.95%), Postives = 282/464 (60.78%), Query Frame = 0


Query: 3   IFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGS 62
           + +L    F     IGI YGR A+NLPS  +V +L++   +  V++YD +  VL+A + +
Sbjct: 13  VSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANT 72

Query: 63  GIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFL 122
           GI++ + +PN  L A A+  +   +W+  N+  YYPST+I +I+VG EV   P N T  +
Sbjct: 73  GIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLV 132

Query: 123 VPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQ 182
           +PAM+NIH AL K  L   IK+SS  +L+ L  S+P S+ SF  +      +PML+FL +
Sbjct: 133 LPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLEFLVE 192

Query: 183 TGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAAMS 242
             S  M++ YP++AY  +++ + L+YALF  +  VVD  +G  Y ++FDAQ+DA++ A++
Sbjct: 193 NESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALT 252

Query: 243 ALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVY 302
           A+ +  +K++VTE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP +P  ++ VY
Sbjct: 253 AMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVY 312

Query: 303 LFALFNENKKTGPASERNYGLFYPNEEKVYDIPFT---------TDGLKDYHDKPSPAPA 362
           LF+LFNEN+K G  SERN+G+FY N   VY + FT         T+        PS +P 
Sbjct: 313 LFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPI 372

Query: 363 AGGDGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDA 422
             G+        G GG  K      WC+AS +A   +LQ +LD+ACG G  DC  +QPD 
Sbjct: 373 INGNSTVTIGGGGGGGTKK------WCIASSQASVTELQTALDWACGPGNVDCSAVQPDQ 432

Query: 423 TCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 458
            C+ P+T+ +HASYAFN+YYQ++G     C F GA+  V + PS
Sbjct: 433 PCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPS 469

[Close]

BLAST of MC04g0089 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 8.0e-98
Identity = 188/455 (41.32%), Postives = 279/455 (61.32%), Query Frame = 0


Query: 5   LLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGSGI 64
           LL L     GG +G+ YGR A++LP+  KVV+L++ H +  V++YD +  VL+A   + I
Sbjct: 14  LLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSI 73

Query: 65  KVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPH-NTTSFLV 124
           ++ + +PN  L A ++  +   +W++ +V  YYP+T+I  I VG E   DPH N +SF+V
Sbjct: 74  ELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVV 133

Query: 125 PAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQT 184
           PAM+N+  AL K  L   IKVS+ ++L  L  S+P SAG+F         RPML+FL + 
Sbjct: 134 PAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA-YFLRPMLEFLAEN 193

Query: 185 GSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAAMSA 244
            S  M++ YP++AY  + + +SLDY LF  +  V+D  +G  Y ++FDAQ+DA++ A++A
Sbjct: 194 KSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTA 253

Query: 245 LKYDDIKMVVTETGWPSKGDENE-IGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVY 304
           L +  IK++VTETGWP+KG   E   AS +NA  YN N++R +++  GTP +P   + VY
Sbjct: 254 LNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVY 313

Query: 305 LFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGHMA 364
           +F+LFNEN+K G  SERN+GLFYP++  VY + FT                   +G H  
Sbjct: 314 IFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFT----------------GKSNGFH-- 373

Query: 365 PVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNTLE 424
             S   G + S + N+WC+AS +A +  L+ +LD+ACG G  DC  IQP   C+ P+TL 
Sbjct: 374 --SNSSGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLV 433

Query: 425 AHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 458
           +HAS+ FNSY+Q+N      C FGGA   V + PS
Sbjct: 434 SHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPS 447

[Close]

BLAST of MC04g0089 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 340.9 bits (873), Expect = 2.3e-92
Identity = 185/460 (40.22%), Postives = 273/460 (59.35%), Query Frame = 0


Query: 3   IFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGS 62
           +FL   S  S    IG+N G    N+PS  +VV LLKS  ++RV++YD D ++L A + +
Sbjct: 9   LFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFAHT 68

Query: 63  GIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFL 122
           G++V + +PN+ L   ++    A +WV +NVAAYYP+T I  IAVG+EV     N  S L
Sbjct: 69  GVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAASVL 128

Query: 123 VPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQ 182
           V A+K I  ALV  NL   IKVS+P + + + +S+P S   F  +  + V  P+L FL+ 
Sbjct: 129 VSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN-KTWDPVIVPLLKFLQS 188

Query: 183 TGSYLMVNAYPFFAYESNSDVISLDYALFRD---NPGVVDSGSGYRYFSLFDAQIDAVFA 242
           TGS L++N YP+F Y  ++ VI LDYALF+    N   VD+ +   Y ++FDA +DA + 
Sbjct: 189 TGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAYF 248

Query: 243 AMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADL 302
           AMS L + +I +VVTE+GWPSKG  +E  A++ENA  YN NL++ +++  GTP  P   +
Sbjct: 249 AMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTAV 308

Query: 303 TVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGG 362
           T Y++ L+NE+ + GP SE+N+GLFY N   VY +                         
Sbjct: 309 TTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTL------------------------- 368

Query: 363 HMAPVSGDGGVSKSQTGN-TWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNP 422
               ++G G +  + T N T+C+A  +  ++ LQA+LD+ACG G  DC  +    +CY P
Sbjct: 369 ---RLAGAGAILANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEP 428

Query: 423 NTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSK 459
           + + AH++YAFN+YYQK G+  G+C F G A V T  PS+
Sbjct: 429 DDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSR 439

[Close]

BLAST of MC04g0089 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 338.6 bits (867), Expect = 1.1e-91
Identity = 180/451 (39.91%), Postives = 263/451 (58.31%), Query Frame = 0


Query: 10  PFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGSGIKVTVD 69
           P  +G  IG+N G   +++P   +VV LLK+  +  +++Y+ DP +L AL+ +GIKV + 
Sbjct: 25  PADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIIS 84

Query: 70  LPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFLVPAMKNI 129
           +PN+ L    +  + A +WV++NV A+YP+T I A++VG+EV     N    LV A+KN+
Sbjct: 85  IPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNV 144

Query: 130 HQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQTGSYLMV 189
           H AL+  NL   IKVS+P++ S + + +P S   F   L   V  P+L FL+ T SYLMV
Sbjct: 145 HAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSL-NAVIVPLLSFLQSTNSYLMV 204

Query: 190 NAYPFFAYESNSDVISLDYALFR---DNPGVVDSGSGYRYFSLFDAQIDAVFAAMSALKY 249
           N YP+  Y  ++ VI LDYALF+    N   VD+ +  RY + FDA +DA + AM+ L +
Sbjct: 205 NVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNF 264

Query: 250 DDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFAL 309
            +I ++VTE+GWPSKG+ NE  A+L+NA  YN NL+R +L+  GTP RP   ++ Y++ L
Sbjct: 265 TNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYEL 324

Query: 310 FNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGHMAPVSG 369
           +NE+ K G  SE+N+GLF  N E VY +  T  G                          
Sbjct: 325 YNEDTKAG-LSEKNWGLFNANGEPVYVLRLTNSG-------------------------- 384

Query: 370 DGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNTLEAHAS 429
              ++   T  T+C A   A  + LQA+LD+ACG G  DC PI+   TCY P+ + AHA+
Sbjct: 385 -SVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHAN 444

Query: 430 YAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 458
           YAF++YY + G     C F G A + T  PS
Sbjct: 445 YAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

[Close]

BLAST of MC04g0089 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 6.2e-90
Identity = 180/461 (39.05%), Postives = 267/461 (57.92%), Query Frame = 0


Query: 2   LIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSG 61
           L+ LL +   S+   IG+N G    N+P    +V LLKS  +  V++YD +  +L+A + 
Sbjct: 11  LLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFAN 70

Query: 62  SGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSF 121
           + I+V V + NE +    +  + A +WV KNVAAY PST I AIAVG+EV     +    
Sbjct: 71  TSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPI 130

Query: 122 LVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLR 181
           L  A+ NIH+ALV  NL+  +KVSSP+++  +   +P S  +F P    TV++ +L FL+
Sbjct: 131 LASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQ-LLQFLK 190

Query: 182 QTGSYLMVNAYPFFAYESNSDVISLDYALFRD-NP--GVVDSGSGYRYFSLFDAQIDAVF 241
            TGS+ M+NAYP++ Y + + +  LDYALF+  +P   +VD  +   Y S+FDA +DA +
Sbjct: 191 NTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAY 250

Query: 242 AAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKAD 301
            +M AL +  I +VVTETGWPS G  +E  A++ NA  +N NL++R+L+  G P +P   
Sbjct: 251 YSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIP 310

Query: 302 LTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDG 361
           +  Y++ L+NE+K++GP SERN+G+ +PN   VY                          
Sbjct: 311 INTYIYELYNEDKRSGPVSERNWGILFPNGTSVY-------------------------- 370

Query: 362 GHMAPVSGDGGVSKSQTGNT--WCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCY 421
               P+S  GG S +    +  +CVA  +A  +KL   L++ACG+G A+C  IQP   CY
Sbjct: 371 ----PLSLSGGSSSAALNGSSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCY 430

Query: 422 NPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 458
            PN +++HAS+AFN YYQK     GTC F G A   T+ PS
Sbjct: 431 LPNDVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of MC04g0089 vs. NCBI nr
Match: XP_038886813.1 (LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida])

HSP 1 Score: 852 bits (2202), Expect = 1.84e-310
Identity = 424/461 (91.97%), Postives = 440/461 (95.44%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F IF L LS F++ GSIG+NYGRIANNLPSAVKVVKLLK+HGL RVKVYDTDPAVLRALS
Sbjct: 9   FFIFPLFLSSFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDTDPAVLRALS 68

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRLTFAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 69  GSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 128

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPML+FL
Sbjct: 129 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLEFL 188

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESN+DVISLDYALFRDNPGVVD+GSGYRYF+LFDAQIDAVFAA
Sbjct: 189 RQTGSYLMVNAYPFFAYESNADVISLDYALFRDNPGVVDAGSGYRYFNLFDAQIDAVFAA 248

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIKMVVTETGWPSKGDENEIGAS+ENAAAYNGNLVRRIL+GGGTPLRPKADLT
Sbjct: 249 MSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILNGGGTPLRPKADLT 308

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GPASERNYGLFYPNEEKVY+IPFTT+GLKDYHDKPSPAP  GG+G  
Sbjct: 309 VYLFALFNENKKNGPASERNYGLFYPNEEKVYEIPFTTEGLKDYHDKPSPAPVTGGNGPS 368

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGG-----ADCRPIQPDATC 420
             P SGDGGVSKSQTGNTWCVASGEAGKEKLQ+SLDYACGEGG     ADCRPIQP ATC
Sbjct: 369 GPPESGDGGVSKSQTGNTWCVASGEAGKEKLQSSLDYACGEGGXXXXXADCRPIQPGATC 428

Query: 421 YNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           YNPNTLEAHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPP
Sbjct: 429 YNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPP 469

[Close]

BLAST of MC04g0089 vs. NCBI nr
Match: KAG6572415.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012014.1 Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 852 bits (2200), Expect = 2.85e-310
Identity = 422/456 (92.54%), Postives = 437/456 (95.83%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F IF+L LSPF++ GSIG+NYGRIANNLPSAVKVVKLLK+HGL RVKVYDTDPAVLRALS
Sbjct: 8   FFIFILFLSPFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDTDPAVLRALS 67

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRLTFAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 68  GSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 127

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYPSSAGSFRPELVE VFRPML+FL
Sbjct: 128 FLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVFRPMLEFL 187

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDAQIDAVFAA
Sbjct: 188 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQIDAVFAA 247

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIKMVVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTPLRPKADLT
Sbjct: 248 MSALKYDDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTPLRPKADLT 307

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAPA GGDG  
Sbjct: 308 VYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAPATGGDG-Q 367

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 420
               SGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP ATCYNPNT
Sbjct: 368 TGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPSATCYNPNT 427

Query: 421 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           L+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 428 LQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MC04g0089 vs. NCBI nr
Match: XP_022952844.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata])

HSP 1 Score: 849 bits (2193), Expect = 3.32e-309
Identity = 421/456 (92.32%), Postives = 437/456 (95.83%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F IFLL LSPF++ GSIG+NYGRIAN+LPSAVKVVKLLKSHGL RVKVYDTDPAVLRALS
Sbjct: 8   FFIFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRALS 67

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 68  GSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 127

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYPSSAGSFRPELVE VFRPML+FL
Sbjct: 128 FLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVFRPMLEFL 187

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDAQIDAVFAA
Sbjct: 188 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQIDAVFAA 247

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIK+VVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTPLRPKADLT
Sbjct: 248 MSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTPLRPKADLT 307

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAPA GGDG  
Sbjct: 308 VYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAPATGGDG-Q 367

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 420
               SGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP ATCYNPNT
Sbjct: 368 AGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPSATCYNPNT 427

Query: 421 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           L+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 428 LQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MC04g0089 vs. NCBI nr
Match: XP_023553980.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846 bits (2186), Expect = 3.87e-308
Identity = 421/456 (92.32%), Postives = 435/456 (95.39%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F IFLL L PF++ GSIG+NYGRIANNLPSAVKVVKLLKSHGL RVKVYDTDPAVLRALS
Sbjct: 8   FFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRALS 67

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRL+FA +WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 68  GSGIKVTVDLPNELLFAAAKRLSFANTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 127

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYPSSAGSFRPELVE VFRPML+FL
Sbjct: 128 FLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVFRPMLEFL 187

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDAQIDAVFAA
Sbjct: 188 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQIDAVFAA 247

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIKMVVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTPLRPKADLT
Sbjct: 248 MSALKYDDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTPLRPKADLT 307

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAPA GGDG  
Sbjct: 308 VYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAPATGGDG-Q 367

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 420
               SGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP ATCYNPNT
Sbjct: 368 TGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPSATCYNPNT 427

Query: 421 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           L+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 428 LQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MC04g0089 vs. NCBI nr
Match: XP_022969398.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima])

HSP 1 Score: 846 bits (2185), Expect = 5.49e-308
Identity = 421/456 (92.32%), Postives = 434/456 (95.18%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F IFLL L PF++ GSIG+NYGRIANNLPSAVKVVKLLKSHGL RVKVYDTDPAVLRALS
Sbjct: 8   FFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRALS 67

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 68  GSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 127

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYPSSAGSFRPELVE VFRPML+FL
Sbjct: 128 FLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVFRPMLEFL 187

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDAQIDAVFAA
Sbjct: 188 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQIDAVFAA 247

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIKMVVTETGWPSKGDENEIGAS  NAAAYNGNL+RRILSGGGTPLRPKADLT
Sbjct: 248 MSALKYDDIKMVVTETGWPSKGDENEIGASAANAAAYNGNLIRRILSGGGTPLRPKADLT 307

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAPA GGDG  
Sbjct: 308 VYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAPATGGDG-Q 367

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 420
                GDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP ATCYNPNT
Sbjct: 368 TGSGHGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPSATCYNPNT 427

Query: 421 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           L+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 428 LQAHASYAFNSYYQKNVRKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MC04g0089 vs. ExPASy TrEMBL
Match: A0A6J1GMW6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 PE=3 SV=1)

HSP 1 Score: 849 bits (2193), Expect = 1.61e-309
Identity = 421/456 (92.32%), Postives = 437/456 (95.83%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F IFLL LSPF++ GSIG+NYGRIAN+LPSAVKVVKLLKSHGL RVKVYDTDPAVLRALS
Sbjct: 8   FFIFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRALS 67

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 68  GSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 127

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYPSSAGSFRPELVE VFRPML+FL
Sbjct: 128 FLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVFRPMLEFL 187

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDAQIDAVFAA
Sbjct: 188 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQIDAVFAA 247

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIK+VVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTPLRPKADLT
Sbjct: 248 MSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTPLRPKADLT 307

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAPA GGDG  
Sbjct: 308 VYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAPATGGDG-Q 367

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 420
               SGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP ATCYNPNT
Sbjct: 368 AGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPSATCYNPNT 427

Query: 421 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           L+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 428 LQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MC04g0089 vs. ExPASy TrEMBL
Match: A0A6J1I0V3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=3 SV=1)

HSP 1 Score: 846 bits (2185), Expect = 2.66e-308
Identity = 421/456 (92.32%), Postives = 434/456 (95.18%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F IFLL L PF++ GSIG+NYGRIANNLPSAVKVVKLLKSHGL RVKVYDTDPAVLRALS
Sbjct: 8   FFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRALS 67

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 68  GSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 127

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYPSSAGSFRPELVE VFRPML+FL
Sbjct: 128 FLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVFRPMLEFL 187

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDAQIDAVFAA
Sbjct: 188 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQIDAVFAA 247

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIKMVVTETGWPSKGDENEIGAS  NAAAYNGNL+RRILSGGGTPLRPKADLT
Sbjct: 248 MSALKYDDIKMVVTETGWPSKGDENEIGASAANAAAYNGNLIRRILSGGGTPLRPKADLT 307

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAPA GGDG  
Sbjct: 308 VYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAPATGGDG-Q 367

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 420
                GDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP ATCYNPNT
Sbjct: 368 TGSGHGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPSATCYNPNT 427

Query: 421 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           L+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 428 LQAHASYAFNSYYQKNVRKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MC04g0089 vs. ExPASy TrEMBL
Match: A0A0A0KEL0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3 SV=1)

HSP 1 Score: 845 bits (2184), Expect = 4.23e-308
Identity = 417/456 (91.45%), Postives = 434/456 (95.18%), Query Frame = 0


Query: 1   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 60
           F  FLL LSPF++ GSIG+NYGRI N+LPSAVKVVKLLKSHGL RVKVYDTDPAVL+ALS
Sbjct: 10  FYFFLLFLSPFAESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDTDPAVLKALS 69

Query: 61  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 120
           GSGIKVTVDLPNELLFAAAKRLTFAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 70  GSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 129

Query: 121 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 180
           FLVPAMKNIHQALVKYNLHS IKVSSPIALSALQNSYPSSAGSFRPELVETVFRPML+FL
Sbjct: 130 FLVPAMKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLEFL 189

Query: 181 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 240
           RQTGSYLMVNAYPFFAYESN+DVISLDYALFRDNPGVVD+GSGYRYF+LFDAQIDAVFAA
Sbjct: 190 RQTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFDAQIDAVFAA 249

Query: 241 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 300
           MSALKYDDIKMVVTETGWPSKGDENEIGAS+ENAAAYNGNLVRRILSGGGTPLRPKADLT
Sbjct: 250 MSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLT 309

Query: 301 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 360
           VYLFALFNENKK GP SERNYGLFYPNEEKVYDIPFTT+GLKD+ DKPSP P +GG+   
Sbjct: 310 VYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDKPSPKPVSGGNAPT 369

Query: 361 MAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 420
             P SGDGGVSKSQTGNTWCVASGEAGKEKLQ+ LDYACGEGGADCRPIQ  ATCYNPNT
Sbjct: 370 APPASGDGGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQVGATCYNPNT 429

Query: 421 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           LEAHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPP
Sbjct: 430 LEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPP 465

[Close]

BLAST of MC04g0089 vs. ExPASy TrEMBL
Match: A0A6J1ERV0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 PE=3 SV=1)

HSP 1 Score: 834 bits (2154), Expect = 1.83e-303
Identity = 415/462 (89.83%), Postives = 435/462 (94.16%), Query Frame = 0


Query: 1   FLIFLLCLSP----FSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVL 60
           F +FLL L+      ++ GSIG+NYGRIANNLPSA+KVV LLKSHGL RVKVYDTDPAVL
Sbjct: 9   FFVFLLLLAASFPVLTEAGSIGVNYGRIANNLPSALKVVNLLKSHGLQRVKVYDTDPAVL 68

Query: 61  RALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPH 120
           RA+SGSGIKVTVDLPNELLFAAAKRLTFAY+WVEKN+ AYYPSTEIEAIAVGNEVFVDPH
Sbjct: 69  RAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVGNEVFVDPH 128

Query: 121 NTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPM 180
           NTTSFL+PAMKNIHQALVKYNLHS+IKVSSPIALSALQNSYPSSAGSFRPEL+E+VFRPM
Sbjct: 129 NTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPM 188

Query: 181 LDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDA 240
           L+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVD+GSGYRYFSLFDAQIDA
Sbjct: 189 LEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDA 248

Query: 241 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPK 300
           VFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS+ENAAAYNGNLVRRILSGGGTPLRPK
Sbjct: 249 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPK 308

Query: 301 ADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP--AA 360
           ADLTVYLFALFNENKK GP SERNYGLFYPNEE VYDIPFTT+GLKDYHDKPSP+P  A 
Sbjct: 309 ADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPAT 368

Query: 361 GGDGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDAT 420
           GGDG    PV+GDG VSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQ  AT
Sbjct: 369 GGDG-KSGPVNGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGAT 428

Query: 421 CYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           CYNPNTLEAHASYAFNSYYQKNGR  GTCYFGGAAYVVTQPP
Sbjct: 429 CYNPNTLEAHASYAFNSYYQKNGRMTGTCYFGGAAYVVTQPP 469

[Close]

BLAST of MC04g0089 vs. ExPASy TrEMBL
Match: A0A6J1JLE7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=3 SV=1)

HSP 1 Score: 832 bits (2148), Expect = 1.50e-302
Identity = 415/462 (89.83%), Postives = 434/462 (93.94%), Query Frame = 0


Query: 1   FLIFLLCLSP----FSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVL 60
           F +FLL LSP     ++ GSIG+NYGRIANNLPSAVKVV LLKSHGL RVKVYDTDPAVL
Sbjct: 9   FFVFLLLLSPSFAVVTEAGSIGVNYGRIANNLPSAVKVVNLLKSHGLQRVKVYDTDPAVL 68

Query: 61  RALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPH 120
            A+SGSGIKVTVDLPNELLFAAAKRLTFAY+WVEKN+ AYYPSTEIEAIAVGNEVFVDPH
Sbjct: 69  TAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVGNEVFVDPH 128

Query: 121 NTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPM 180
           NTTSFL+PAMKNIHQALVKYNLHS+IKVSSPIALSALQNSYPSSAGSFRPEL+E+VFRPM
Sbjct: 129 NTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPM 188

Query: 181 LDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDA 240
           L+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVD+GSGYRYFSLFDAQIDA
Sbjct: 189 LEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDA 248

Query: 241 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPK 300
           VFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS+ NAAAYNGNLVRRILSGGGTPLRPK
Sbjct: 249 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVGNAAAYNGNLVRRILSGGGTPLRPK 308

Query: 301 ADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP--AA 360
           ADLTVYLFALFNENKK GP SERNYGLFYPNEE VYDIPFTT+GLKDYHDKPSP+P  A 
Sbjct: 309 ADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPAT 368

Query: 361 GGDGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDAT 420
           GGDG    PV+GDG VSKSQ+GNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQ  AT
Sbjct: 369 GGDG-KSGPVNGDGNVSKSQSGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGAT 428

Query: 421 CYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 456
           CYNPNTLEAHASYAFNSYYQKNGRK GTCYFGGAAYVVTQ P
Sbjct: 429 CYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQQP 469

[Close]

BLAST of MC04g0089 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 709.1 bits (1829), Expect = 2.3e-204
Identity = 347/457 (75.93%), Postives = 404/457 (88.40%), Query Frame = 0


Query: 2   LIFLLCLSPF--SDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRAL 61
           +  LL LSPF  SD GSIG+NYGRI++ LPSA KVV+LLKS G+ RVK++D DP+VL+AL
Sbjct: 8   IFLLLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKAL 67

Query: 62  SGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTT 121
           SGSGIKVTVDLPNELLF+AAKR +FA SWV++NVAAY+PST+IE+IAVGNEVFVD HNTT
Sbjct: 68  SGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNTT 127

Query: 122 SFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDF 181
           SFL+PAM+NIH+AL+ +NLHS IK+SSP+ALSALQNSYPSS+GSFRPEL+++V +PMLDF
Sbjct: 128 SFLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPMLDF 187

Query: 182 LRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFA 241
           LR+TGS LM+N YPFFAYE NSDVI LDYAL R+NPG+VDSG+G RYF+LFDAQIDAVFA
Sbjct: 188 LRETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDAQIDAVFA 247

Query: 242 AMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADL 301
           AMSALKYDDI+++VTETGWPSKGDENE+GA+L NAA+YNGNL+RRIL+ GGTPLRPKADL
Sbjct: 248 AMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTPLRPKADL 307

Query: 302 TVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGG 361
           TVYLFALFNENKK GP SERNYGLF+P+E+KVYDIPFTT+GLK Y D     P  GGD  
Sbjct: 308 TVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRD-GGHTPVTGGDQV 367

Query: 362 HMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPN 421
              P+S  GGVSKS  G TWCVA+G+AG+E+LQ  LDYACGEGGADCRPIQP A CY+P+
Sbjct: 368 TKPPMS--GGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPD 427

Query: 422 TLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 457
           TLEAHAS+AFNSYYQK GR  G+CYFGGAAYVV+QPP
Sbjct: 428 TLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPP 461

[Close]

BLAST of MC04g0089 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 598.2 bits (1541), Expect = 5.6e-171
Identity = 300/465 (64.52%), Postives = 357/465 (76.77%), Query Frame = 0


Query: 1   FLIFLLCLSPFS-------DGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDP 60
           F++ LL LS FS       D G IG+NYGRIA+NLP+  KVV+LLK+ G++R+K+YDT+ 
Sbjct: 4   FVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTET 63

Query: 61  AVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFV 120
            VL AL+ SGIKV V LPNE L +AA   ++  +WV+ N+  Y P+T+IEAIAVGNEVFV
Sbjct: 64  TVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFV 123

Query: 121 DPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVF 180
           DP NTT++LVPAMKN+  +LVK+NL  +IK+SSPIALSAL +SYP SAGSF+PEL+E V 
Sbjct: 124 DPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVI 183

Query: 181 RPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQ 240
           +PMLD LR+T S+LMVNAYPFFAY +N+D ISLDYALF++N G VDSG+G +Y SL DAQ
Sbjct: 184 KPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQ 243

Query: 241 IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPL 300
           IDAVFAAMSA+ ++D+K+VVTETGWPS GDENEIGA   NAAAYNG LV+R+L+G GTPL
Sbjct: 244 IDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPL 303

Query: 301 RPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPA 360
           +PK  L VYLFALFNEN+KTGP SERNYGLFYPNE KVYD+           +K    P 
Sbjct: 304 KPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKVVP- 363

Query: 361 AGGDGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDA 420
                           V  S  G TWCVA+G+  KEKLQ  LDYACGEGGADCRPIQP A
Sbjct: 364 ----------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGA 423

Query: 421 TCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSK 459
           TCYNP +LEAHASYAFNSYYQKN R +GTC FGGAAYVV+QPPSK
Sbjct: 424 TCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPSK 451

[Close]

BLAST of MC04g0089 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 594.7 bits (1532), Expect = 6.2e-170
Identity = 298/463 (64.36%), Postives = 355/463 (76.67%), Query Frame = 0


Query: 1   FLIFLLCLSPFS-------DGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDP 60
           F++ LL LS FS       D G IG+NYGRIA+NLP+  KVV+LLK+ G++R+K+YDT+ 
Sbjct: 4   FVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTET 63

Query: 61  AVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFV 120
            VL AL+ SGIKV V LPNE L +AA   ++  +WV+ N+  Y P+T+IEAIAVGNEVFV
Sbjct: 64  TVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFV 123

Query: 121 DPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVF 180
           DP NTT++LVPAMKN+  +LVK+NL  +IK+SSPIALSAL +SYP SAGSF+PEL+E V 
Sbjct: 124 DPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVI 183

Query: 181 RPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQ 240
           +PMLD LR+T S+LMVNAYPFFAY +N+D ISLDYALF++N G VDSG+G +Y SL DAQ
Sbjct: 184 KPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQ 243

Query: 241 IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPL 300
           IDAVFAAMSA+ ++D+K+VVTETGWPS GDENEIGA   NAAAYNG LV+R+L+G GTPL
Sbjct: 244 IDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPL 303

Query: 301 RPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPA 360
           +PK  L VYLFALFNEN+KTGP SERNYGLFYPNE KVYD+           +K    P 
Sbjct: 304 KPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKVVP- 363

Query: 361 AGGDGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDA 420
                           V  S  G TWCVA+G+  KEKLQ  LDYACGEGGADCRPIQP A
Sbjct: 364 ----------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGA 423

Query: 421 TCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 457
           TCYNP +LEAHASYAFNSYYQKN R +GTC FGGAAYVV+QPP
Sbjct: 424 TCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPP 449

[Close]

BLAST of MC04g0089 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 580.9 bits (1496), Expect = 9.3e-166
Identity = 298/460 (64.78%), Postives = 356/460 (77.39%), Query Frame = 0


Query: 1   FLIFLLCLSPFS----DGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVL 60
           FL + L LS  S      G +G+NYGRIANNLPS  KVV LLKS G++R+K++DTD  VL
Sbjct: 5   FLPYFLILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVL 64

Query: 61  RALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPH 120
            AL+ S IKV V LPNELL +AA   +FA +W++ ++  Y+P+TEIEAIAVGNEVFVDP 
Sbjct: 65  TALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP- 124

Query: 121 NTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPM 180
             T +LV AMKNIH +LVKY L  AIK+SSPIALSAL NSYP S+GSF+PEL+E V +PM
Sbjct: 125 TITPYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPM 184

Query: 181 LDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDA 240
           L  L+QT SYLMVNAYPFFAY +N+D ISLDYALF++N G +DSG+G +Y SLFDAQIDA
Sbjct: 185 LALLQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDA 244

Query: 241 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPK 300
           V+AA+SA+ +  +K++VTETGWPS GDENEIGAS  NAAAYN  LV+R+L+G GTPLRP 
Sbjct: 245 VYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPT 304

Query: 301 ADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGG 360
             L VYLFALFNEN+K GP SERNYGLFYPNE KVY++PFT         K S  P  G 
Sbjct: 305 EPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFT---------KKSTTPVNGN 364

Query: 361 DGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCY 420
            G    PV+ +        G+TWCV++GE  KEKLQ +LDYACGEGGADCRPIQP ATCY
Sbjct: 365 RG--KVPVTHE--------GHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCY 424

Query: 421 NPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 457
           +P +LEAHASYAFNSYYQKN R++GTC+FGGAA+VVTQPP
Sbjct: 425 HPESLEAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPP 444

[Close]

BLAST of MC04g0089 vs. TAIR 10
Match: AT4G29360.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 368.6 bits (945), Expect = 7.2e-102
Identity = 192/466 (41.20%), Postives = 284/466 (60.94%), Query Frame = 0


Query: 3   IFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGS 62
           + +L    F     IGI YGR A+NLPS  +V +L++   +  V++YD +  VL+A + +
Sbjct: 13  VSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANT 72

Query: 63  GIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFL 122
           GI++ + +PN  L A A+  +   +W+  N+  YYPST+I +I+VG EV   P N T  +
Sbjct: 73  GIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLV 132

Query: 123 VPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQ 182
           +PAM+NIH AL K  L   IK+SS  +L+ L  S+P S+ SF  +      +PML+FL +
Sbjct: 133 LPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLEFLVE 192

Query: 183 TGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAAMS 242
             S  M++ YP++AY  +++ + L+YALF  +  VVD  +G  Y ++FDAQ+DA++ A++
Sbjct: 193 NESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALT 252

Query: 243 ALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVY 302
           A+ +  +K++VTE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP +P  ++ VY
Sbjct: 253 AMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVY 312

Query: 303 LFALFNENKKTGPASERNYGLFYPNEEKVYDIPFT---------TDGLKDYHDKPSPAPA 362
           LF+LFNEN+K G  SERN+G+FY N   VY + FT         T+        PS +P 
Sbjct: 313 LFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPI 372

Query: 363 AGGDGGHMAPVSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDA 422
             G+        G GG  K      WC+AS +A   +LQ +LD+ACG G  DC  +QPD 
Sbjct: 373 INGNSTVTIGGGGGGGTKK------WCIASSQASVTELQTALDWACGPGNVDCSAVQPDQ 432

Query: 423 TCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSKY 460
            C+ P+T+ +HASYAFN+YYQ++G     C F GA+  V + PSKY
Sbjct: 433 PCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSKY 471

The following BLAST results are available for this feature:

BLAST of MC04g0089 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	2.5e-99	40.95	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	8.0e-98	41.32	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q9ZU91	2.3e-92	40.22	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9C7U5	1.1e-91	39.91	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
Q94CD8	6.2e-90	39.05	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]

BLAST of MC04g0089 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886813.1	1.84e-310	91.97	LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida]	[more]
KAG6572415.1	2.85e-310	92.54	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022952844.1	3.32e-309	92.32	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata]	[more]
XP_023553980.1	3.87e-308	92.32	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022969398.1	5.49e-308	92.32	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima]	[more]

BLAST of MC04g0089 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMW6	1.61e-309	92.32	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 P...	[more]
A0A6J1I0V3	2.66e-308	92.32	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=...	[more]
A0A0A0KEL0	4.23e-308	91.45	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3...	[more]
A0A6J1ERV0	1.83e-303	89.83	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 P...	[more]
A0A6J1JLE7	1.50e-302	89.83	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=...	[more]

BLAST of MC04g0089 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G05790.1	2.3e-204	75.93	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.2	5.6e-171	64.52	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.1	6.2e-170	64.36	O-Glycosyl hydrolases family 17 protein	[more]
AT4G26830.1	9.3e-166	64.78	O-Glycosyl hydrolases family 17 protein	[more]
AT4G29360.2	7.2e-102	41.20	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 378..459 e-value: 5.1E-44 score: 162.2
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 378..449 e-value: 1.1E-22 score: 80.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 15..345 e-value: 2.6E-126 score: 422.9
None	No IPR available	GENE3D	1.20.58.1040		coord: 372..460 e-value: 2.6E-11 score: 45.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 343..370
None	No IPR available	PANTHER	PTHR32227:SF350	O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN-RELATED	coord: 4..458
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 17..336 e-value: 1.6E-90 score: 303.9
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 249..262
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 4..458
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 17..335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0089.1	MC04g0089.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds