MC04g0080 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0080 vs. ExPASy Swiss-Prot
Match: Q67ZI9 (GDSL esterase/lipase At2g42990 OS=Arabidopsis thaliana OX=3702 GN=At2g42990 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-127
Identity = 212/364 (58.24%), Postives = 274/364 (75.27%), Query Frame = 0

Query: 5   HFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGG 64
           H+L   +L I++ TL S  GAK+PAIIVFGDSSVDSGNNN + T+ ++NF PYGRDF GG
Sbjct: 4   HYLSPSILCIILTTLVSIAGAKIPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGG 63

Query: 65  QATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVL 124
           +ATGRF NGR+  DF SEA+GLK T+PAYLDP Y I+DFATGVCFASAGTG+DNST+DVL
Sbjct: 64  RATGRFCNGRLSSDFTSEAYGLKPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVL 123

Query: 125 NSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLG 184
                              VIPLW+EVE+FK+YQ+ L AY+G+ +A ++IRE+LY+VS+G
Sbjct: 124 ------------------GVIPLWKEVEYFKEYQSNLSAYLGHRRAAKIIRESLYIVSIG 183

Query: 185 TNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLE 244
           TNDFLENYYT+P RR QF+I QY+DFL+E+A  F+ ++Y LGARK+SFTG+ PMGCLPLE
Sbjct: 184 TNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYRLGARKMSFTGISPMGCLPLE 243

Query: 245 RATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYL 304
           R TN+   F C   YN +A++FN +L   V  LN +L G+ + FANP+ I + I+++P L
Sbjct: 244 RVTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTGIKIYFANPYDIMWDIVTKPNL 303

Query: 305 YGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRIL 364
           YG E++  ACC TG FEM +LC Q+NP TC DANK+VFWDAFHPT++TNQI+ +   + L
Sbjct: 304 YGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFWDAFHPTERTNQIVSDHFFKHL 349

Query: 365 LPMF 369
             +F
Sbjct: 364 KNLF 349

BLAST of MC04g0080 vs. ExPASy Swiss-Prot
Match: Q8VY93 (GDSL esterase/lipase At4g26790 OS=Arabidopsis thaliana OX=3702 GN=At4g26790 PE=2 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 4.3e-118
Identity = 200/359 (55.71%), Postives = 263/359 (73.26%), Query Frame = 0

Query: 11  LLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRF 70
           LL   +     +T AK PA+IVFGDS+VDSGNNN + TVLKSNF+PYGRD+  G+ATGRF
Sbjct: 11  LLAAQLLVKIPETCAKFPALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKATGRF 70

Query: 71  SNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLM 130
           SNGR+ PDFISE  GLK+ +PAYLDP Y IADFATGVCFASAGTG DN+TS VL      
Sbjct: 71  SNGRIAPDFISEGLGLKNAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVL------ 130

Query: 131 YNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLE 190
                       +V+PLW+EVE++K+YQ +LR+Y+G EKANE+I E+LYL+S+GTNDFLE
Sbjct: 131 ------------SVMPLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLE 190

Query: 191 NYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVM 250
           NYY +PR+  ++++ +Y+ FL+ +A  F+ ++Y LGARK+S +GL P GCLPLER T + 
Sbjct: 191 NYYLLPRKLRKYSVNEYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQLF 250

Query: 251 GNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVA 310
               C+E+YN VA +FN K+E  V  LN  L G+ ++F+NP+ +  +II  P  +GFE  
Sbjct: 251 YGSKCIEEYNIVARDFNIKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENV 310

Query: 311 GKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMFR 370
             ACC TG +EMSYLC++ NPFTC DA+KYVFWD+FHPT+KTN I+ N +L+  L  F+
Sbjct: 311 RSACCGTGYYEMSYLCDKMNPFTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRFQ 351

BLAST of MC04g0080 vs. ExPASy Swiss-Prot
Match: Q9SJB4 (GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 6.2e-117
Identity = 201/368 (54.62%), Postives = 261/368 (70.92%), Query Frame = 0

Query: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60
           MG    L   L +I + +  +  G K+PAIIVFGDSSVD+GNNN + TV +SNF PYGRD
Sbjct: 1   MGHLKSLFTILFLIAMSSTVTFAG-KIPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRD 60

Query: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120
           FVGG+ TGRF NG++  DF+SEA GLK  IPAYLDP Y I+DFATGV FASA TG+DN+T
Sbjct: 61  FVGGKPTGRFCNGKIATDFMSEALGLKPIIPAYLDPSYNISDFATGVTFASAATGYDNAT 120

Query: 121 SDVLNSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180
           SDVL                  +V+PLW+++E++K+YQ KL+AY G ++  E I  +LYL
Sbjct: 121 SDVL------------------SVLPLWKQLEYYKEYQTKLKAYQGKDRGTETIESSLYL 180

Query: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240
           +S+GTNDFLENY+  P R  Q+++  Y+DFL  +A +F+ +L+GLGARKIS  GLPPMGC
Sbjct: 181 ISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHGLGARKISLGGLPPMGC 240

Query: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 300
           +PLERATN+     CV +YN +A++FN KL+  V  L+ +LPG  ++F+NP+  F +II 
Sbjct: 241 MPLERATNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPGSNLVFSNPYEPFMRIIK 300

Query: 301 QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL 360
            P  +GFEV G ACCATG FEM Y C + NPFTC +A+KYVFWD+FHPTQKTN I+ N L
Sbjct: 301 NPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFWDSFHPTQKTNHIMANAL 349

Query: 361 LRILLPMF 369
           +    P F
Sbjct: 361 MNSTFPHF 349

BLAST of MC04g0080 vs. ExPASy Swiss-Prot
Match: Q9FHW9 (GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana OX=3702 GN=At5g42170/At5g42160 PE=3 SV=2)

HSP 1 Score: 279.3 bits (713), Expect = 6.5e-74
Identity = 142/344 (41.28%), Postives = 200/344 (58.14%), Query Frame = 0

Query: 18  TLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPP 77
           T+       +P II FGDS VDSGNNN ++T LK NF PYG+DF G  ATGRFS+GRVP 
Sbjct: 39  TIKLPPNVTIPGIITFGDSIVDSGNNNHLRTALKCNFPPYGKDFPGKIATGRFSDGRVPS 98

Query: 78  DFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLMYNEDSVI 137
           D ++E  G+  TIPAYL+P     D   GV FAS G+G+D  T+ ++             
Sbjct: 99  DIVAERLGIAETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLV------------- 158

Query: 138 YETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLENYYTIPR 197
                 V+ L  +++ F++Y+NKL+  +G EKAN +++ +LYLV   +ND    Y     
Sbjct: 159 -----KVVSLSDQLKNFQEYKNKLKVIVGEEKANFLVKNSLYLVVASSNDIAHTY---TA 218

Query: 198 RRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVMGNFGCVE 257
           R +++    Y D+L + A KF+  LYGLGAR+I      P+GC+P  R         C E
Sbjct: 219 RSIKYNKTSYADYLADSASKFVSALYGLGARRIGVFSAVPVGCVPAARTLRGKLKRRCSE 278

Query: 258 KYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVAGKACCAT 317
           K N VA  FN K+   + AL  +LP   ++  +       +I  P  YGFEV+ + CC T
Sbjct: 279 KLNEVARNFNAKISPTLEALGKELPDSRVVLIDVCDTLNDMIENPKNYGFEVSNRGCCGT 338

Query: 318 GTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLL 362
           G  E+ +LCN+ NPFTC +++ Y+FWD++HPT+K  QIIV+ LL
Sbjct: 339 GLVEVLFLCNKINPFTCKNSSSYIFWDSYHPTEKAYQIIVDKLL 361

BLAST of MC04g0080 vs. ExPASy Swiss-Prot
Match: Q9C653 (GDSL esterase/lipase At1g58525 OS=Arabidopsis thaliana OX=3702 GN=At1g58525 PE=3 SV=2)

HSP 1 Score: 276.2 bits (705), Expect = 5.5e-73
Identity = 145/360 (40.28%), Postives = 217/360 (60.28%), Query Frame = 0

Query: 7   LVNPLLVILIFTLASKTG--AKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGG 66
           L+  L++I +   A K G  A +PA+IVFGDS +D+GNNN + T+LK NF PYG+D+ GG
Sbjct: 6   LLLALVLIAVEANAVKQGINATIPALIVFGDSIMDTGNNNNLPTLLKCNFPPYGKDYPGG 65

Query: 67  QATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVL 126
            ATGRFS+GRVP D I+E  GL  T+PAY++      D   GV FAS GTG+D  T+ ++
Sbjct: 66  DATGRFSDGRVPSDLIAEKLGLAKTLPAYMNSYLKPEDLLKGVTFASRGTGYDPLTAKIM 125

Query: 127 NSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLG 186
                             +VI +W ++ +FK+Y +K++ + G EKA +++  + +LV   
Sbjct: 126 ------------------SVISVWDQLIYFKEYISKIKRHFGEEKAKDILEHSFFLVVSS 185

Query: 187 TNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLE 246
           +ND    Y     R   +    Y +FL + A  F+ EL+ LGARKI      P+GC+PL+
Sbjct: 186 SNDLAHTYLAQAHR---YDRTSYANFLADSAVHFVRELHKLGARKIGVFSAVPVGCVPLQ 245

Query: 247 RATNVMGNF---GCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQ 306
           R   V G F   GC E  N +A +FN +L   + +L+ +L G+ +L+ N +   + +I  
Sbjct: 246 R--TVFGGFFTRGCNEPLNNMAKQFNARLSPALDSLDKELDGV-ILYINVYDTLFDMIQH 305

Query: 307 PYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLL 362
           P  YGF+VA K CC  G   +SYLCN  NPFTC +++ Y+FWD++HP+++  Q+IV++LL
Sbjct: 306 PKKYGFDVADKGCCGKGLLTISYLCNSLNPFTCSNSSSYIFWDSYHPSERAYQVIVDNLL 341

BLAST of MC04g0080 vs. NCBI nr
Match: XP_022147832.1 (GDSL esterase/lipase At2g42990-like isoform X1 [Momordica charantia])

HSP 1 Score: 701 bits (1810), Expect = 1.92e-254
Identity = 351/369 (95.12%), Postives = 351/369 (95.12%), Query Frame = 0

Query: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60
           MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD
Sbjct: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60

Query: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120
           FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST
Sbjct: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120

Query: 121 SDVLNSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180
           SDVLN                  VIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL
Sbjct: 121 SDVLN------------------VIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180

Query: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240
           VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC
Sbjct: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240

Query: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 300
           LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS
Sbjct: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 300

Query: 301 QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL 360
           QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL
Sbjct: 301 QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL 351

Query: 361 LRILLPMFR 369
           LRILLPMFR
Sbjct: 361 LRILLPMFR 351

BLAST of MC04g0080 vs. NCBI nr
Match: XP_022930712.1 (GDSL esterase/lipase At2g42990-like [Cucurbita moschata])

HSP 1 Score: 584 bits (1505), Expect = 4.89e-208
Identity = 288/363 (79.34%), Postives = 312/363 (85.95%), Query Frame = 0

Query: 7   LVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQA 66
           LV+ +  I+ FT  SK  AKVPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ 
Sbjct: 3   LVSIIFSIIFFTWGSKIEAKVPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQP 62

Query: 67  TGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNS 126
           TGRFSNGRVPPDFISEAFGLK TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLN 
Sbjct: 63  TGRFSNGRVPPDFISEAFGLKPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLN- 122

Query: 127 IMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTN 186
                            VIPLWREVE +K+YQ KLR Y+GNEKANEVI+EALYLVSLGTN
Sbjct: 123 -----------------VIPLWREVELYKEYQAKLRGYLGNEKANEVIKEALYLVSLGTN 182

Query: 187 DFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERA 246
           DFLENYYTIPRRRLQF++QQYEDFLL+LAGKFIGE+Y LG RKIS +GLPPMGCLPLERA
Sbjct: 183 DFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIGEIYSLGVRKISISGLPPMGCLPLERA 242

Query: 247 TNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYG 306
           TNVM NF CVEK+N VALEFN KLEGFV ALN QLPGLTMLF+NP+ IFYQII+QPYLYG
Sbjct: 243 TNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGLTMLFSNPYAIFYQIITQPYLYG 302

Query: 307 FEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLP 366
           FEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWDAFHPT+KTNQIIVNDLL+ LLP
Sbjct: 303 FEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWDAFHPTEKTNQIIVNDLLQTLLP 347

Query: 367 MFR 369
            FR
Sbjct: 363 TFR 347

BLAST of MC04g0080 vs. NCBI nr
Match: XP_023529877.1 (GDSL esterase/lipase At2g42990-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583 bits (1502), Expect = 1.40e-207
Identity = 286/356 (80.34%), Postives = 308/356 (86.52%), Query Frame = 0

Query: 14  ILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNG 73
           I+ FT  SK  AKVPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ TGRFSNG
Sbjct: 10  IIFFTWGSKIEAKVPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQPTGRFSNG 69

Query: 74  RVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLMYNE 133
           RVPPDFISEAFGLK TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLN        
Sbjct: 70  RVPPDFISEAFGLKPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLN-------- 129

Query: 134 DSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLENYY 193
                     VIPLWREVE +K+YQ KLR Y+GNEKANEVIREALYLVSLGTNDFLENYY
Sbjct: 130 ----------VIPLWREVELYKEYQAKLRGYLGNEKANEVIREALYLVSLGTNDFLENYY 189

Query: 194 TIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVMGNF 253
           TIPRRRLQF++QQYEDFLL+LAGKFIGE+Y LG RKIS +GLPPMGCLPLERATNVM NF
Sbjct: 190 TIPRRRLQFSVQQYEDFLLQLAGKFIGEIYSLGVRKISISGLPPMGCLPLERATNVMSNF 249

Query: 254 GCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVAGKA 313
            CVEK+N VALEFN KLEGFV ALN QLPGLTMLF+NP+ IFYQII++PYLYGFEVAGKA
Sbjct: 250 ECVEKFNLVALEFNAKLEGFVWALNRQLPGLTMLFSNPYAIFYQIITKPYLYGFEVAGKA 309

Query: 314 CCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMFR 369
           CC TGTFEMSYLC+QEN  TC DA KYVFWDAFHPT+KTNQIIVNDLL+ LLP FR
Sbjct: 310 CCGTGTFEMSYLCSQENSLTCSDATKYVFWDAFHPTEKTNQIIVNDLLQTLLPTFR 347

BLAST of MC04g0080 vs. NCBI nr
Match: KAG6589280.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583 bits (1502), Expect = 1.40e-207
Identity = 287/363 (79.06%), Postives = 313/363 (86.23%), Query Frame = 0

Query: 7   LVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQA 66
           LV+ +  I+ FT  SK  AKVPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ 
Sbjct: 3   LVSIIFSIIFFTWGSKIEAKVPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQP 62

Query: 67  TGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNS 126
           TGRFSNGRVPPDFISEAFGLK TIPAYLDP +TIADFATGVCFASAGTGFD++TSDVLN 
Sbjct: 63  TGRFSNGRVPPDFISEAFGLKPTIPAYLDPAFTIADFATGVCFASAGTGFDDATSDVLN- 122

Query: 127 IMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTN 186
                            VIPLWREVE +K+YQ KLR Y+GNEKANEVI+EALYLVSLGTN
Sbjct: 123 -----------------VIPLWREVELYKEYQAKLRGYLGNEKANEVIKEALYLVSLGTN 182

Query: 187 DFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERA 246
           DFLENYYTIPRRRLQF++QQYEDFLL+LAGKFIGE+Y LG RKIS +GLPPMGCLPLERA
Sbjct: 183 DFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIGEIYSLGVRKISISGLPPMGCLPLERA 242

Query: 247 TNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYG 306
           TNVM NF CVEK+N VALEFN KLEGFV ALN QLPGLTMLF+NP+ IFYQII++PYLYG
Sbjct: 243 TNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGLTMLFSNPYAIFYQIITKPYLYG 302

Query: 307 FEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLP 366
           FEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWDAFHPT+KTNQIIVNDLL+ LLP
Sbjct: 303 FEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWDAFHPTEKTNQIIVNDLLQTLLP 347

Query: 367 MFR 369
           MFR
Sbjct: 363 MFR 347

BLAST of MC04g0080 vs. NCBI nr
Match: XP_022989111.1 (GDSL esterase/lipase At2g42990-like [Cucurbita maxima])

HSP 1 Score: 579 bits (1493), Expect = 3.29e-206
Identity = 285/356 (80.06%), Postives = 307/356 (86.24%), Query Frame = 0

Query: 14  ILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNG 73
           I+ FT  SK  AKVPAIIVFGDSSVDSGNNN +KT+LKSNF PYGRDF GGQ TGRFSNG
Sbjct: 10  IIFFTWGSKIEAKVPAIIVFGDSSVDSGNNNAIKTLLKSNFMPYGRDFYGGQPTGRFSNG 69

Query: 74  RVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLMYNE 133
           RVPPDFISEAFGLK TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLN        
Sbjct: 70  RVPPDFISEAFGLKPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLN-------- 129

Query: 134 DSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLENYY 193
                     VIPLWREVE +K+YQ KLR Y+GNEKANEVI+EALYLVSLGTNDFLENYY
Sbjct: 130 ----------VIPLWREVELYKEYQAKLRGYVGNEKANEVIKEALYLVSLGTNDFLENYY 189

Query: 194 TIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVMGNF 253
           TIPRRRLQF++QQYEDFLL+LAGKFI E+Y LG RKIS +GLPPMGCLPLERATNVM NF
Sbjct: 190 TIPRRRLQFSVQQYEDFLLQLAGKFIREIYSLGVRKISISGLPPMGCLPLERATNVMSNF 249

Query: 254 GCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVAGKA 313
            CVEK+N VALEFN KLEGFV ALN QLPGLTMLF+NP+ IFYQII++PYLYGFEVAGKA
Sbjct: 250 ECVEKFNLVALEFNAKLEGFVWALNRQLPGLTMLFSNPYAIFYQIITKPYLYGFEVAGKA 309

Query: 314 CCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMFR 369
           CC TGTFEMSYLC+QEN  TC DA KYVFWDAFHPT+KTNQIIVNDLL+ LLPMFR
Sbjct: 310 CCGTGTFEMSYLCSQENSLTCSDATKYVFWDAFHPTEKTNQIIVNDLLQTLLPMFR 347

BLAST of MC04g0080 vs. ExPASy TrEMBL
Match: A0A6J1D3F6 (GDSL esterase/lipase At2g42990-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016676 PE=3 SV=1)

HSP 1 Score: 701 bits (1810), Expect = 9.31e-255
Identity = 351/369 (95.12%), Postives = 351/369 (95.12%), Query Frame = 0

Query: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60
           MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD
Sbjct: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60

Query: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120
           FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST
Sbjct: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120

Query: 121 SDVLNSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180
           SDVLN                  VIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL
Sbjct: 121 SDVLN------------------VIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180

Query: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240
           VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC
Sbjct: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240

Query: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 300
           LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS
Sbjct: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 300

Query: 301 QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL 360
           QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL
Sbjct: 301 QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL 351

Query: 361 LRILLPMFR 369
           LRILLPMFR
Sbjct: 361 LRILLPMFR 351

BLAST of MC04g0080 vs. ExPASy TrEMBL
Match: A0A6J1ERD0 (GDSL esterase/lipase At2g42990-like OS=Cucurbita moschata OX=3662 GN=LOC111437110 PE=3 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 2.37e-208
Identity = 288/363 (79.34%), Postives = 312/363 (85.95%), Query Frame = 0

Query: 7   LVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQA 66
           LV+ +  I+ FT  SK  AKVPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ 
Sbjct: 3   LVSIIFSIIFFTWGSKIEAKVPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQP 62

Query: 67  TGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNS 126
           TGRFSNGRVPPDFISEAFGLK TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLN 
Sbjct: 63  TGRFSNGRVPPDFISEAFGLKPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLN- 122

Query: 127 IMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTN 186
                            VIPLWREVE +K+YQ KLR Y+GNEKANEVI+EALYLVSLGTN
Sbjct: 123 -----------------VIPLWREVELYKEYQAKLRGYLGNEKANEVIKEALYLVSLGTN 182

Query: 187 DFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERA 246
           DFLENYYTIPRRRLQF++QQYEDFLL+LAGKFIGE+Y LG RKIS +GLPPMGCLPLERA
Sbjct: 183 DFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIGEIYSLGVRKISISGLPPMGCLPLERA 242

Query: 247 TNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYG 306
           TNVM NF CVEK+N VALEFN KLEGFV ALN QLPGLTMLF+NP+ IFYQII+QPYLYG
Sbjct: 243 TNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGLTMLFSNPYAIFYQIITQPYLYG 302

Query: 307 FEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLP 366
           FEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWDAFHPT+KTNQIIVNDLL+ LLP
Sbjct: 303 FEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWDAFHPTEKTNQIIVNDLLQTLLP 347

Query: 367 MFR 369
            FR
Sbjct: 363 TFR 347

BLAST of MC04g0080 vs. ExPASy TrEMBL
Match: A0A6J1JJ45 (GDSL esterase/lipase At2g42990-like OS=Cucurbita maxima OX=3661 GN=LOC111486280 PE=3 SV=1)

HSP 1 Score: 579 bits (1493), Expect = 1.59e-206
Identity = 285/356 (80.06%), Postives = 307/356 (86.24%), Query Frame = 0

Query: 14  ILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNG 73
           I+ FT  SK  AKVPAIIVFGDSSVDSGNNN +KT+LKSNF PYGRDF GGQ TGRFSNG
Sbjct: 10  IIFFTWGSKIEAKVPAIIVFGDSSVDSGNNNAIKTLLKSNFMPYGRDFYGGQPTGRFSNG 69

Query: 74  RVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLMYNE 133
           RVPPDFISEAFGLK TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLN        
Sbjct: 70  RVPPDFISEAFGLKPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLN-------- 129

Query: 134 DSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLENYY 193
                     VIPLWREVE +K+YQ KLR Y+GNEKANEVI+EALYLVSLGTNDFLENYY
Sbjct: 130 ----------VIPLWREVELYKEYQAKLRGYVGNEKANEVIKEALYLVSLGTNDFLENYY 189

Query: 194 TIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVMGNF 253
           TIPRRRLQF++QQYEDFLL+LAGKFI E+Y LG RKIS +GLPPMGCLPLERATNVM NF
Sbjct: 190 TIPRRRLQFSVQQYEDFLLQLAGKFIREIYSLGVRKISISGLPPMGCLPLERATNVMSNF 249

Query: 254 GCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVAGKA 313
            CVEK+N VALEFN KLEGFV ALN QLPGLTMLF+NP+ IFYQII++PYLYGFEVAGKA
Sbjct: 250 ECVEKFNLVALEFNAKLEGFVWALNRQLPGLTMLFSNPYAIFYQIITKPYLYGFEVAGKA 309

Query: 314 CCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMFR 369
           CC TGTFEMSYLC+QEN  TC DA KYVFWDAFHPT+KTNQIIVNDLL+ LLPMFR
Sbjct: 310 CCGTGTFEMSYLCSQENSLTCSDATKYVFWDAFHPTEKTNQIIVNDLLQTLLPMFR 347

BLAST of MC04g0080 vs. ExPASy TrEMBL
Match: A0A6J1D2E2 (GDSL esterase/lipase At2g42990-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016676 PE=3 SV=1)

HSP 1 Score: 567 bits (1462), Expect = 1.02e-202
Identity = 288/306 (94.12%), Postives = 288/306 (94.12%), Query Frame = 0

Query: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60
           MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD
Sbjct: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60

Query: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120
           FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST
Sbjct: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120

Query: 121 SDVLNSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180
           SDVLN                  VIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL
Sbjct: 121 SDVLN------------------VIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180

Query: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240
           VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC
Sbjct: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240

Query: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 300
           LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS
Sbjct: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 288

Query: 301 QPYLYG 306
           QPYLYG
Sbjct: 301 QPYLYG 288

BLAST of MC04g0080 vs. ExPASy TrEMBL
Match: A0A0A0KGK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G421680 PE=3 SV=1)

HSP 1 Score: 564 bits (1454), Expect = 1.28e-200
Identity = 276/358 (77.09%), Postives = 306/358 (85.47%), Query Frame = 0

Query: 11  LLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRF 70
           LL++L+ T  + T +K+PA+IVFGDSSVDSGNNNV+KT+LKSNFRPYGRDF+ GQ TGRF
Sbjct: 5   LLLLLLCTTFTLTSSKIPAVIVFGDSSVDSGNNNVIKTLLKSNFRPYGRDFLSGQPTGRF 64

Query: 71  SNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLM 130
           SNG+VPPDFISEAFGLK TIPAYLDP +TIADFATGVCFASAGTGFDNSTSDVLN     
Sbjct: 65  SNGKVPPDFISEAFGLKPTIPAYLDPAFTIADFATGVCFASAGTGFDNSTSDVLN----- 124

Query: 131 YNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLE 190
                        VIP+W+EVE FK+YQ KLR Y+GNEKANEVI+EALYLVSLGTNDFLE
Sbjct: 125 -------------VIPMWKEVELFKEYQRKLRGYLGNEKANEVIKEALYLVSLGTNDFLE 184

Query: 191 NYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVM 250
           NYYT P+RRLQF+IQQ+EDFLL+LA  FI +L+  GARKISFTGLPPMGCLPLERATNVM
Sbjct: 185 NYYTFPQRRLQFSIQQFEDFLLDLARNFIKQLHNDGARKISFTGLPPMGCLPLERATNVM 244

Query: 251 GNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVA 310
           GNF CV+KYN VALEFN KLE FV  LN QLPGLTM+F+NP+PIFYQII+ PYL+G+EVA
Sbjct: 245 GNFDCVDKYNLVALEFNNKLEAFVSDLNTQLPGLTMIFSNPYPIFYQIITNPYLFGYEVA 304

Query: 311 GKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMF 368
           GKACC TGTFEMSYLCNQEN FTC DANKYVFWDAFHPTQKTNQIIVN LL  LL  F
Sbjct: 305 GKACCGTGTFEMSYLCNQENSFTCPDANKYVFWDAFHPTQKTNQIIVNHLLPSLLSTF 344

BLAST of MC04g0080 vs. TAIR 10
Match: AT2G42990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 457.6 bits (1176), Expect = 9.5e-129
Identity = 212/364 (58.24%), Postives = 274/364 (75.27%), Query Frame = 0

Query: 5   HFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGG 64
           H+L   +L I++ TL S  GAK+PAIIVFGDSSVDSGNNN + T+ ++NF PYGRDF GG
Sbjct: 4   HYLSPSILCIILTTLVSIAGAKIPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGG 63

Query: 65  QATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVL 124
           +ATGRF NGR+  DF SEA+GLK T+PAYLDP Y I+DFATGVCFASAGTG+DNST+DVL
Sbjct: 64  RATGRFCNGRLSSDFTSEAYGLKPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVL 123

Query: 125 NSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLG 184
                              VIPLW+EVE+FK+YQ+ L AY+G+ +A ++IRE+LY+VS+G
Sbjct: 124 ------------------GVIPLWKEVEYFKEYQSNLSAYLGHRRAAKIIRESLYIVSIG 183

Query: 185 TNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLE 244
           TNDFLENYYT+P RR QF+I QY+DFL+E+A  F+ ++Y LGARK+SFTG+ PMGCLPLE
Sbjct: 184 TNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYRLGARKMSFTGISPMGCLPLE 243

Query: 245 RATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYL 304
           R TN+   F C   YN +A++FN +L   V  LN +L G+ + FANP+ I + I+++P L
Sbjct: 244 RVTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTGIKIYFANPYDIMWDIVTKPNL 303

Query: 305 YGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRIL 364
           YG E++  ACC TG FEM +LC Q+NP TC DANK+VFWDAFHPT++TNQI+ +   + L
Sbjct: 304 YGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFWDAFHPTERTNQIVSDHFFKHL 349

Query: 365 LPMF 369
             +F
Sbjct: 364 KNLF 349

BLAST of MC04g0080 vs. TAIR 10
Match: AT4G26790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 426.0 bits (1094), Expect = 3.0e-119
Identity = 200/359 (55.71%), Postives = 263/359 (73.26%), Query Frame = 0

Query: 11  LLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRF 70
           LL   +     +T AK PA+IVFGDS+VDSGNNN + TVLKSNF+PYGRD+  G+ATGRF
Sbjct: 11  LLAAQLLVKIPETCAKFPALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKATGRF 70

Query: 71  SNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLM 130
           SNGR+ PDFISE  GLK+ +PAYLDP Y IADFATGVCFASAGTG DN+TS VL      
Sbjct: 71  SNGRIAPDFISEGLGLKNAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVL------ 130

Query: 131 YNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLE 190
                       +V+PLW+EVE++K+YQ +LR+Y+G EKANE+I E+LYL+S+GTNDFLE
Sbjct: 131 ------------SVMPLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLE 190

Query: 191 NYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVM 250
           NYY +PR+  ++++ +Y+ FL+ +A  F+ ++Y LGARK+S +GL P GCLPLER T + 
Sbjct: 191 NYYLLPRKLRKYSVNEYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQLF 250

Query: 251 GNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVA 310
               C+E+YN VA +FN K+E  V  LN  L G+ ++F+NP+ +  +II  P  +GFE  
Sbjct: 251 YGSKCIEEYNIVARDFNIKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENV 310

Query: 311 GKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMFR 370
             ACC TG +EMSYLC++ NPFTC DA+KYVFWD+FHPT+KTN I+ N +L+  L  F+
Sbjct: 311 RSACCGTGYYEMSYLCDKMNPFTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRFQ 351

BLAST of MC04g0080 vs. TAIR 10
Match: AT4G26790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 426.0 bits (1094), Expect = 3.0e-119
Identity = 200/359 (55.71%), Postives = 263/359 (73.26%), Query Frame = 0

Query: 11  LLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRF 70
           LL   +     +T AK PA+IVFGDS+VDSGNNN + TVLKSNF+PYGRD+  G+ATGRF
Sbjct: 11  LLAAQLLVKIPETCAKFPALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKATGRF 70

Query: 71  SNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLM 130
           SNGR+ PDFISE  GLK+ +PAYLDP Y IADFATGVCFASAGTG DN+TS VL      
Sbjct: 71  SNGRIAPDFISEGLGLKNAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVL------ 130

Query: 131 YNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLE 190
                       +V+PLW+EVE++K+YQ +LR+Y+G EKANE+I E+LYL+S+GTNDFLE
Sbjct: 131 ------------SVMPLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLE 190

Query: 191 NYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVM 250
           NYY +PR+  ++++ +Y+ FL+ +A  F+ ++Y LGARK+S +GL P GCLPLER T + 
Sbjct: 191 NYYLLPRKLRKYSVNEYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQLF 250

Query: 251 GNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVA 310
               C+E+YN VA +FN K+E  V  LN  L G+ ++F+NP+ +  +II  P  +GFE  
Sbjct: 251 YGSKCIEEYNIVARDFNIKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENV 310

Query: 311 GKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMFR 370
             ACC TG +EMSYLC++ NPFTC DA+KYVFWD+FHPT+KTN I+ N +L+  L  F+
Sbjct: 311 RSACCGTGYYEMSYLCDKMNPFTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRFQ 351

BLAST of MC04g0080 vs. TAIR 10
Match: AT2G04570.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 4.4e-118
Identity = 201/368 (54.62%), Postives = 261/368 (70.92%), Query Frame = 0

Query: 1   MGSFHFLVNPLLVILIFTLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRD 60
           MG    L   L +I + +  +  G K+PAIIVFGDSSVD+GNNN + TV +SNF PYGRD
Sbjct: 1   MGHLKSLFTILFLIAMSSTVTFAG-KIPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRD 60

Query: 61  FVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNST 120
           FVGG+ TGRF NG++  DF+SEA GLK  IPAYLDP Y I+DFATGV FASA TG+DN+T
Sbjct: 61  FVGGKPTGRFCNGKIATDFMSEALGLKPIIPAYLDPSYNISDFATGVTFASAATGYDNAT 120

Query: 121 SDVLNSIMLMYNEDSVIYETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYL 180
           SDVL                  +V+PLW+++E++K+YQ KL+AY G ++  E I  +LYL
Sbjct: 121 SDVL------------------SVLPLWKQLEYYKEYQTKLKAYQGKDRGTETIESSLYL 180

Query: 181 VSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGC 240
           +S+GTNDFLENY+  P R  Q+++  Y+DFL  +A +F+ +L+GLGARKIS  GLPPMGC
Sbjct: 181 ISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHGLGARKISLGGLPPMGC 240

Query: 241 LPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIIS 300
           +PLERATN+     CV +YN +A++FN KL+  V  L+ +LPG  ++F+NP+  F +II 
Sbjct: 241 MPLERATNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPGSNLVFSNPYEPFMRIIK 300

Query: 301 QPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDL 360
            P  +GFEV G ACCATG FEM Y C + NPFTC +A+KYVFWD+FHPTQKTN I+ N L
Sbjct: 301 NPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFWDSFHPTQKTNHIMANAL 349

Query: 361 LRILLPMF 369
           +    P F
Sbjct: 361 MNSTFPHF 349

BLAST of MC04g0080 vs. TAIR 10
Match: AT5G42170.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 4.6e-75
Identity = 142/344 (41.28%), Postives = 200/344 (58.14%), Query Frame = 0

Query: 18  TLASKTGAKVPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPP 77
           T+       +P II FGDS VDSGNNN ++T LK NF PYG+DF G  ATGRFS+GRVP 
Sbjct: 39  TIKLPPNVTIPGIITFGDSIVDSGNNNHLRTALKCNFPPYGKDFPGKIATGRFSDGRVPS 98

Query: 78  DFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNSIMLMYNEDSVI 137
           D ++E  G+  TIPAYL+P     D   GV FAS G+G+D  T+ ++             
Sbjct: 99  DIVAERLGIAETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLV------------- 158

Query: 138 YETEQNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLENYYTIPR 197
                 V+ L  +++ F++Y+NKL+  +G EKAN +++ +LYLV   +ND    Y     
Sbjct: 159 -----KVVSLSDQLKNFQEYKNKLKVIVGEEKANFLVKNSLYLVVASSNDIAHTY---TA 218

Query: 198 RRLQFTIQQYEDFLLELAGKFIGELYGLGARKISFTGLPPMGCLPLERATNVMGNFGCVE 257
           R +++    Y D+L + A KF+  LYGLGAR+I      P+GC+P  R         C E
Sbjct: 219 RSIKYNKTSYADYLADSASKFVSALYGLGARRIGVFSAVPVGCVPAARTLRGKLKRRCSE 278

Query: 258 KYNAVALEFNRKLEGFVGALNMQLPGLTMLFANPFPIFYQIISQPYLYGFEVAGKACCAT 317
           K N VA  FN K+   + AL  +LP   ++  +       +I  P  YGFEV+ + CC T
Sbjct: 279 KLNEVARNFNAKISPTLEALGKELPDSRVVLIDVCDTLNDMIENPKNYGFEVSNRGCCGT 338

Query: 318 GTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLL 362
           G  E+ +LCN+ NPFTC +++ Y+FWD++HPT+K  QIIV+ LL
Sbjct: 339 GLVEVLFLCNKINPFTCKNSSSYIFWDSYHPTEKAYQIIVDKLL 361