MC04g0049 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0049
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein ROOT PRIMORDIUM DEFECTIVE 1
LocationMC04: 398169 .. 402248 (-)
RNA-Seq ExpressionMC04g0049
SyntenyMC04g0049
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTGATGCTGTTGCGTGGAATTCCATGATTATGGGTGTGGCTAAATGTGGGGAAATTGGGGAGTCGAGGAAGCTGTTCGATACAATGCCCGAAAGAAACCCAATCTCATGGAATTCCATGATTGGTGGGTATGTTAGAAATGGGATGTTCAAAGAAGCCCTGAAGCTGTTTGTCAAAATGCAAGAAGAGAAAATCAAGCCTAGTGAGTTTACAATGGTGAGCTTGTTAAACGCCTCAGCTCACATTGGAGCACTCAGGCAAGGGGAATGGATTCATGAGTACATAGAGAAGAACAATCTAGAGTTGAATCCCATTGTAGTTACAGCAATCATAGACATGTACTGCAAATGTGGTAGCATAGTTAATGCCCTTCAAGTGTTTGAGAAAATGCCCAGCAGAGGATTATCTAGTTGGAACTCCATGATCTTCGGCCTGGCTGTGAATGGCTGCGAAAATGAAGCAATTTTGTTATTCAGAATGCTGGAATCTTCGAGTCTTGAACCAGATTCTGTTAGTTTTATGGCTGTATTAACAGCTTGTAATCACGCTGCAATGGTGGAAGAAGGCAGGGAAATTTTCTCACTGATGATAAACACTTATAGAATCAAACCATCAATAAAACACTACAATTTAATGGTGGACATGATCAGCCGAGCTGGGTTTCTTGAAGAAGCAGAGCAGCTCATAAAAACCATGCCGATTGAGGCAGATGCAATCATGTGGGGGTGTTTACTCTCAGCTTGTAGAACGCGTGGGAACACAGAAATAGCGAAAAGGGCAGCGGAGAAAGTTAATGAACTGGATCCAGAAGAAAGCTGTGGTCATGTTCTTATGGCGAATGTTCATGCTTGGACCAGTAACTTTGTGGGAGCAATGGAGGAAAGGGTTTCAATGAGGATGAAGAATGTGGAGAAAGAACCAGGGGGAAGCCTGATTGAAGTTGATGGTGAAGTTCATGAGTTTATAGCTGGTGGTGGGAGGTTGCATCCTAAAGCTCAAGAGATTTACATTGTATTGGACCAATTGGGAGTGATGCAACAAGACACTGGAAATTTATTGATTAATCTGAAAGAAATGAATCATTTGGAGGTTTAGATTTTTAATCCCTTAATGCTTCACAACATTTTCATCTACTACCCTTTGATGACCTTGGAGTGAAGGTTATATTTTTATTGGCTAACCACCTTTCTTAAAGTATTTGTCAATATTCATTTGATATAATAGAAATAAGAGTTTTTTTTTTTTTTACATAAATACTGCTAATAACTACAGAAATATGCACCTTCAACTTCTATTGTTATGTGTTCTTTTTGTTTGTTTATATATATTTATATTTATATTTATATTTAGTACAACAACGGGAGTGTGGGAATTCGAACTCACAACTTCACGATTAGAGATAGGTGTCAATACCGCTAAGCTTAGTTTATATTGACGCTCATTTTGTTTATACAAGGAATTGATTTACTTTTACTATTTTTTAATAAAACACCCATGGATTTATCTTAAATATGAGTTGATTTAGTATAACAAAGTCTATGATGTTGTGAAAGTTATTATTTTATTTAAAGCATTAACATTAATTGCAATCGCAGTAAAAAAAGAAAAAAAAACCATTAATTGCAATGAACTATTGGAAATTCCATTCAATATCCCTATTTATACGGTGGATTTGAAAAATATCCAAAGGCTAACCCAATAATAATCTCATAATTCAAATTCAGGCCCAAGAAGTTCTGACGTAAGCGGGCAGAGGTTTCGAGCTCAATTGGGCCTGACATAGGCCCAAACATTGTTTAGGGCTCCACCTCCAGGGAAGCGCAGACAGTTGGGTCTGACATTTCAGCCCAAATTGTCTAGGACTCGAAATCCGCGGAAGCACAGACGATTGGGCCCAAACATTGTCTACGACTCCAACACCAACGAAGCAGAGACAATTGAGCCTGACATTTGGGCCCAAACATCTTCTAGGGCGGACCCAGAAGGAGCACAGATAATTGGGCCTAATATTTCAGTCCAAACTGTCTAGGGCTCGAACTGCGGGAGCACATTCGAATGGGCCTGACATTTCAGCCCAAACTTGTCTAGGGCTCCAAAACGAAGAGTCAAATCCTTCCCGTTCGCACACTCTTCGCACGTGCTGTGCCTCCCTCCTTCTCCAGTCTCCACTTCAATTTTCTTTATCAGCAGCAGTTTGTTTCGGTCGCCATTCCAAAGGCAAAAAAATGGTGGGATCAAAAGTTTCATATGCAGACTTGGTTCGCCAATCGAAAAGTAATAACGTTTCAGAAGCAGTGCTTCGCGAGCTCGGCGCCATGCTTTTCGAGTAAGCTTCTTGCCTTTAAACAACTCTTCTGTCTAATCTCTTTCCATCATAACCTTCGCGGTTCTTCCATGAACTTTGAAACTTTCCCAAAAAAATTATATGAAATTAGATTGCGGCTTGCTTTCTTGTCAATAACATTAGGCATTCTTCTGCTCATGCTTTTTTTTTCTGTTTTTTCCCCTCTTGCAATTATATTTCAGTACATCTCCAAATTTCATTAATTATTTACTGTTGAGAATTTCAGATAGCTTGGTCCTCATTTTTGCAAGCTGAATTAAAGAAACGCCAAAGACAGTGAAGCTAAGGAGAGGTGTACAATGTTTTTGTTATCGCGCAATCAATCCCAACAGCAACACCACCTTTTCCATTTATACATTCGAACGTATGTAGACGGCACCATAAAATGGGTTCGAGATCGTGGTCTTGATCATGCTGTTGAGAGAGAGAAGAATCTTCTGCCAGTGATGAACATTAAAGATTTTATAAAATCAGAGCCGTCCAAGTCTGTCCCAGTTTCCATCATAACGCAGAAAAGGGAAGTTCTAAGAATTCCAACGCGCCCCATTGATTTGATCAGAAAATATCCCTCTATTTTCGAAGAATTTCTCCCTGGAGGGATTGGCATTCAACCTCATGTTCGGCTAACCTCTCAAGTCCTTGAGCTTGATGCCGAAGAACAGTTAAGCTATCAAGGCGAAAATTGTAGACAACGAGCTGCAGATCAACTTGTGAAGCTCTTAATGATTTCAAGATTCCACAAAATACCTTTAAGTATCATTGATCAATTGAAATGGGACCTGGGTCTTCCCAAGGATTACGTCCAAAGTATAGTTCCTGATTTTCCAGATTACTTCAAGGTTGTAGGACACCAAAATTTTGCATCTGGATCAGGAGATACGCGAGCGTTGGAATTGGTATGTTGGAACAATGAATTGGCCACCTCAGTTTTGGAGAAGATGGCTGCGAAGGCAGAAACCGGCGTTTCGAAGGGAATGCCTATTAGTTTTCCTATGAAATTCTCGAATGGGTTTGAGATGGATAAGAAGTTCAAGAAGTGGGTGGATGAATGGCAAAAGCTACCTTACATTTCCCCTTATGAAAATGCATCACATCTCTCTCCGAGCAGCGATGAATCGGACAAGTGGGCGGTTGCGATCTTACATGAGCTTCTTCATATGCTCATTGCAAAGAAGATAGAGAAGGAAACTATACTGTCCATTGGCGAATATTTTGGCCTTCGGTCGAGGTTCAAGAGAGCATTGCTTCACCATCCAGGGATATTCTACCTATCGAGTAAGAATGGAACATACACGGTTGTCTTAAAAGAGGGGTATAAAAGGGGTTCCGTAATTGAGAACAGCCCTTTGATAAACATTAGAAATAAGTACCTTCACCTTATGAATACAGTTAAGGAAGACAGTAAAACAACAACCGAACAAGGCGGTACTTGTCAACAGAAGAAAGAGGAGAAAGAACGGTCAGATGATGCTACTCGAAAAGAGAACGAAGCTGAACTCCTCGACTCTTCGGACGAAGAAGCTGAAGACGATAGTAGTAATCTCAATGCCCGTGCTGATCGGAAAGAGGCTGCTGGTGGACGTAGGAGACATGTTGATTTCCAAGAAAATGTTGGTAGAAACAGAGTGAGATCATCGAAGAGAATGAACTCGACTTCGGGAAGGCCTTCAAGGGATGTTAAAAGAGAGAGGTCAAATAACAGTAAGGGATACCAGGTGAGGACGAAGGTT

mRNA sequence

ATGGAATTTGATGCTGTTGCGTGGAATTCCATGATTATGGGTGTGGCTAAATGTGGGGAAATTGGGGAGTCGAGGAAGCTGTTCGATACAATGCCCGAAAGAAACCCAATCTCATGGAATTCCATGATTGGTGGGTATGTTAGAAATGGGATGTTCAAAGAAGCCCTGAAGCTGTTTGTCAAAATGCAAGAAGAGAAAATCAAGCCTAGTGAGTTTACAATGGTGAGCTTGTTAAACGCCTCAGCTCACATTGGAGCACTCAGGCAAGGGGAATGGATTCATGAGTACATAGAGAAGAACAATCTAGAGTTGAATCCCATTGTAGTTACAGCAATCATAGACATGTACTGCAAATGTGGTAGCATAGTTAATGCCCTTCAAGTGTTTGAGAAAATGCCCAGCAGAGGATTATCTAGTTGGAACTCCATGATCTTCGGCCTGGCTGTGAATGGCTGCGAAAATGAAGCAATTTTGTTATTCAGAATGCTGGAATCTTCGAGTCTTGAACCAGATTCTGTTACAATGGAGGAAAGGGTTTCAATGAGGATGAAGAATGTGGAGAAAGAACCAGGGGGAAGCCTGATTGAAGTTGATGGTGAAGTTCATGAGTTTATAGCTGGTGGTGGGAGGTTGCATCCTAAAGCTCAAGAGATTTACATTGTATTGGACCAATTGGGAGAGAGGTGTACAATGTTTTTGTTATCGCGCAATCAATCCCAACAGCAACACCACCTTTTCCATTTATACATTCGAACGTATGTAGACGGCACCATAAAATGGGTTCGAGATCGTGGTCTTGATCATGCTGTTGAGAGAGAGAAGAATCTTCTGCCAGTGATGAACATTAAAGATTTTATAAAATCAGAGCCGTCCAAGTCTGTCCCAGTTTCCATCATAACGCAGAAAAGGGAAGTTCTAAGAATTCCAACGCGCCCCATTGATTTGATCAGAAAATATCCCTCTATTTTCGAAGAATTTCTCCCTGGAGGGATTGGCATTCAACCTCATGTTCGGCTAACCTCTCAAGTCCTTGAGCTTGATGCCGAAGAACAGTTAAGCTATCAAGGCGAAAATTGTAGACAACGAGCTGCAGATCAACTTGTGAAGCTCTTAATGATTTCAAGATTCCACAAAATACCTTTAAGTATCATTGATCAATTGAAATGGGACCTGGGTCTTCCCAAGGATTACGTCCAAAGTATAGTTCCTGATTTTCCAGATTACTTCAAGGTTGTAGGACACCAAAATTTTGCATCTGGATCAGGAGATACGCGAGCGTTGGAATTGGTATGTTGGAACAATGAATTGGCCACCTCAGTTTTGGAGAAGATGGCTGCGAAGGCAGAAACCGGCGTTTCGAAGGGAATGCCTATTAGTTTTCCTATGAAATTCTCGAATGGGTTTGAGATGGATAAGAAGTTCAAGAAGTGGGTGGATGAATGGCAAAAGCTACCTTACATTTCCCCTTATGAAAATGCATCACATCTCTCTCCGAGCAGCGATGAATCGGACAAGTGGGCGGTTGCGATCTTACATGAGCTTCTTCATATGCTCATTGCAAAGAAGATAGAGAAGGAAACTATACTGTCCATTGGCGAATATTTTGGCCTTCGGTCGAGGTTCAAGAGAGCATTGCTTCACCATCCAGGGATATTCTACCTATCGAGTAAGAATGGAACATACACGGTTGTCTTAAAAGAGGGGTATAAAAGGGGTTCCGTAATTGAGAACAGCCCTTTGATAAACATTAGAAATAAGTACCTTCACCTTATGAATACAGTTAAGGAAGACAGTAAAACAACAACCGAACAAGGCGGTACTTGTCAACAGAAGAAAGAGGAGAAAGAACGGTCAGATGATGCTACTCGAAAAGAGAACGAAGCTGAACTCCTCGACTCTTCGGACGAAGAAGCTGAAGACGATAGTAGTAATCTCAATGCCCGTGCTGATCGGAAAGAGGCTGCTGGTGGACGTAGGAGACATGTTGATTTCCAAGAAAATGTTGGTAGAAACAGAGTGAGATCATCGAAGAGAATGAACTCGACTTCGGGAAGGCCTTCAAGGGATGTTAAAAGAGAGAGGTCAAATAACAGTAAGGGATACCAGGTGAGGACGAAGGTT

Coding sequence (CDS)

ATGGAATTTGATGCTGTTGCGTGGAATTCCATGATTATGGGTGTGGCTAAATGTGGGGAAATTGGGGAGTCGAGGAAGCTGTTCGATACAATGCCCGAAAGAAACCCAATCTCATGGAATTCCATGATTGGTGGGTATGTTAGAAATGGGATGTTCAAAGAAGCCCTGAAGCTGTTTGTCAAAATGCAAGAAGAGAAAATCAAGCCTAGTGAGTTTACAATGGTGAGCTTGTTAAACGCCTCAGCTCACATTGGAGCACTCAGGCAAGGGGAATGGATTCATGAGTACATAGAGAAGAACAATCTAGAGTTGAATCCCATTGTAGTTACAGCAATCATAGACATGTACTGCAAATGTGGTAGCATAGTTAATGCCCTTCAAGTGTTTGAGAAAATGCCCAGCAGAGGATTATCTAGTTGGAACTCCATGATCTTCGGCCTGGCTGTGAATGGCTGCGAAAATGAAGCAATTTTGTTATTCAGAATGCTGGAATCTTCGAGTCTTGAACCAGATTCTGTTACAATGGAGGAAAGGGTTTCAATGAGGATGAAGAATGTGGAGAAAGAACCAGGGGGAAGCCTGATTGAAGTTGATGGTGAAGTTCATGAGTTTATAGCTGGTGGTGGGAGGTTGCATCCTAAAGCTCAAGAGATTTACATTGTATTGGACCAATTGGGAGAGAGGTGTACAATGTTTTTGTTATCGCGCAATCAATCCCAACAGCAACACCACCTTTTCCATTTATACATTCGAACGTATGTAGACGGCACCATAAAATGGGTTCGAGATCGTGGTCTTGATCATGCTGTTGAGAGAGAGAAGAATCTTCTGCCAGTGATGAACATTAAAGATTTTATAAAATCAGAGCCGTCCAAGTCTGTCCCAGTTTCCATCATAACGCAGAAAAGGGAAGTTCTAAGAATTCCAACGCGCCCCATTGATTTGATCAGAAAATATCCCTCTATTTTCGAAGAATTTCTCCCTGGAGGGATTGGCATTCAACCTCATGTTCGGCTAACCTCTCAAGTCCTTGAGCTTGATGCCGAAGAACAGTTAAGCTATCAAGGCGAAAATTGTAGACAACGAGCTGCAGATCAACTTGTGAAGCTCTTAATGATTTCAAGATTCCACAAAATACCTTTAAGTATCATTGATCAATTGAAATGGGACCTGGGTCTTCCCAAGGATTACGTCCAAAGTATAGTTCCTGATTTTCCAGATTACTTCAAGGTTGTAGGACACCAAAATTTTGCATCTGGATCAGGAGATACGCGAGCGTTGGAATTGGTATGTTGGAACAATGAATTGGCCACCTCAGTTTTGGAGAAGATGGCTGCGAAGGCAGAAACCGGCGTTTCGAAGGGAATGCCTATTAGTTTTCCTATGAAATTCTCGAATGGGTTTGAGATGGATAAGAAGTTCAAGAAGTGGGTGGATGAATGGCAAAAGCTACCTTACATTTCCCCTTATGAAAATGCATCACATCTCTCTCCGAGCAGCGATGAATCGGACAAGTGGGCGGTTGCGATCTTACATGAGCTTCTTCATATGCTCATTGCAAAGAAGATAGAGAAGGAAACTATACTGTCCATTGGCGAATATTTTGGCCTTCGGTCGAGGTTCAAGAGAGCATTGCTTCACCATCCAGGGATATTCTACCTATCGAGTAAGAATGGAACATACACGGTTGTCTTAAAAGAGGGGTATAAAAGGGGTTCCGTAATTGAGAACAGCCCTTTGATAAACATTAGAAATAAGTACCTTCACCTTATGAATACAGTTAAGGAAGACAGTAAAACAACAACCGAACAAGGCGGTACTTGTCAACAGAAGAAAGAGGAGAAAGAACGGTCAGATGATGCTACTCGAAAAGAGAACGAAGCTGAACTCCTCGACTCTTCGGACGAAGAAGCTGAAGACGATAGTAGTAATCTCAATGCCCGTGCTGATCGGAAAGAGGCTGCTGGTGGACGTAGGAGACATGTTGATTTCCAAGAAAATGTTGGTAGAAACAGAGTGAGATCATCGAAGAGAATGAACTCGACTTCGGGAAGGCCTTCAAGGGATGTTAAAAGAGAGAGGTCAAATAACAGTAAGGGATACCAGGTGAGGACGAAGGTT

Protein sequence

MEFDAVAWNSMIMGVAKCGEIGESRKLFDTMPERNPISWNSMIGGYVRNGMFKEALKLFVKMQEEKIKPSEFTMVSLLNASAHIGALRQGEWIHEYIEKNNLELNPIVVTAIIDMYCKCGSIVNALQVFEKMPSRGLSSWNSMIFGLAVNGCENEAILLFRMLESSSLEPDSVTMEERVSMRMKNVEKEPGGSLIEVDGEVHEFIAGGGRLHPKAQEIYIVLDQLGERCTMFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEMDKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRDVKRERSNNSKGYQVRTKV
Homology
BLAST of MC04g0049 vs. ExPASy Swiss-Prot
Match: Q9SJG6 (Pentatricopeptide repeat-containing protein At2g42920, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-E75 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.0e-59
Identity = 112/172 (65.12%), Postives = 130/172 (75.58%), Query Frame = 0

Query: 3   FDAVAWNSMIMGVAKCGEIGESRKLFDTMPERNPISWNSMIGGYVRNGMFKEALKLFVKM 62
           FD VAWNSMIMG AKCG I +++ LFD MP+RN +SWNSMI G+VRNG FK+AL +F +M
Sbjct: 190 FDVVAWNSMIMGFAKCGLIDQAQNLFDEMPQRNGVSWNSMISGFVRNGRFKDALDMFREM 249

Query: 63  QEEKIKPSEFTMVSLLNASAHIGALRQGEWIHEYIEKNNLELNPIVVTAIIDMYCKCGSI 122
           QE+ +KP  FTMVSLLNA A++GA  QG WIHEYI +N  ELN IVVTA+IDMYCKCG I
Sbjct: 250 QEKDVKPDGFTMVSLLNACAYLGASEQGRWIHEYIVRNRFELNSIVVTALIDMYCKCGCI 309

Query: 123 VNALQVFEKMPSRGLSSWNSMIFGLAVNGCENEAILLFRMLESSSLEPDSVT 175
              L VFE  P + LS WNSMI GLA NG E  A+ LF  LE S LEPDSV+
Sbjct: 310 EEGLNVFECAPKKQLSCWNSMILGLANNGFEERAMDLFSELERSGLEPDSVS 361

BLAST of MC04g0049 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 4.0e-56
Identity = 127/343 (37.03%), Postives = 194/343 (56.56%), Query Frame = 0

Query: 251 RTYVDGTIKWVRDRGLDHA--VEREKNLLPVMNIKDFIKSEPSKSVPVSIITQKREVLRI 310
           RTYVD  +KW RD   D+   + R   L  V+++K+ I  EP++ +P+S I++K     +
Sbjct: 22  RTYVDVYMKWKRDPYFDNIEHILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTRQFDV 81

Query: 311 PTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQGENCRQRAADQLV 370
            T+    +RK+PSIFEEF+     + P  RLT +  ELD +E++ YQ         D+L 
Sbjct: 82  STKIAHFLRKFPSIFEEFVGPEYNL-PWFRLTPEATELDRQERVVYQ--TSADDLRDRLK 141

Query: 371 KLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQNFASGSGDTRALE 430
           KL+++S+ + +PLSI+  +KW LGLP DY+Q     FPD   +     F       + L 
Sbjct: 142 KLILMSKDNVLPLSIVQGMKWYLGLPDDYLQ-----FPD-MNLDSSFRFVDMEDGVKGLA 201

Query: 431 LVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEMDKKFKKWVDEWQKLPYIS 490
           +     +   SVL+K A K   G      I FP+  S G  +  K + W+ E+QKLPY+S
Sbjct: 202 VDYNGGDKVLSVLQKNAMKKRRGEVSLEEIEFPLFPSKGCRLRVKIEDWLMEFQKLPYVS 261

Query: 491 PYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYFGLRSRFKRALLHH 550
           PY++ S L PSSD ++K  V  LHELL + +    E++ +L + ++FGL  +  +A   H
Sbjct: 262 PYDDYSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKAFERH 321

Query: 551 PGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLM 592
           P IFYLS KN T T +L+E Y+  + +E  P++ +R KY+ LM
Sbjct: 322 PQIFYLSMKNKTCTAILREPYRDKASVETHPVLGVRKKYIQLM 355

BLAST of MC04g0049 vs. ExPASy Swiss-Prot
Match: Q38959 (Pentatricopeptide repeat-containing protein At3g26630, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-A6 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 2.6e-39
Identity = 76/170 (44.71%), Postives = 115/170 (67.65%), Query Frame = 0

Query: 6   VAWNSMIMGVAKCGEIGESRKLFDTMPERNPISWNSMIGGYVRNGMFKEALKLFVKMQEE 65
           V+W +M+ G+    ++  +  +F+ MP RN +SW +MI  YV+N    EA +LF +MQ +
Sbjct: 186 VSWTTMLYGLVSNSQLDSAEIVFNQMPMRNVVSWTAMITAYVKNRRPDEAFQLFRRMQVD 245

Query: 66  KIKPSEFTMVSLLNASAHIGALRQGEWIHEYIEKNNLELNPIVVTAIIDMYCKCGSIVNA 125
            +KP+EFT+V+LL AS  +G+L  G W+H+Y  KN   L+  + TA+IDMY KCGS+ +A
Sbjct: 246 DVKPNEFTIVNLLQASTQLGSLSMGRWVHDYAHKNGFVLDCFLGTALIDMYSKCGSLQDA 305

Query: 126 LQVFEKMPSRGLSSWNSMIFGLAVNGCENEAILLF-RMLESSSLEPDSVT 175
            +VF+ M  + L++WNSMI  L V+GC  EA+ LF  M E +S+EPD++T
Sbjct: 306 RKVFDVMQGKSLATWNSMITSLGVHGCGEEALSLFEEMEEEASVEPDAIT 355

BLAST of MC04g0049 vs. ExPASy Swiss-Prot
Match: Q9LSB8 (Putative pentatricopeptide repeat-containing protein At3g15930 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E51 PE=3 SV=2)

HSP 1 Score: 160.6 bits (405), Expect = 6.4e-38
Identity = 76/171 (44.44%), Postives = 109/171 (63.74%), Query Frame = 0

Query: 4   DAVAWNSMIMGVAKCGEIGESRKLFDTMPERNPISWNSMIGGYVRNGMFKEALKLFVKMQ 63
           D ++W S++ G  + G +  +R  FD MP R+ ISW  MI GY+R G F E+L++F +MQ
Sbjct: 301 DVISWTSIVKGYVERGNLKLARTYFDQMPVRDRISWTIMIDGYLRAGCFNESLEIFREMQ 360

Query: 64  EEKIKPSEFTMVSLLNASAHIGALRQGEWIHEYIEKNNLELNPIVVTAIIDMYCKCGSIV 123
              + P EFTMVS+L A AH+G+L  GEWI  YI+KN ++ + +V  A+IDMY KCG   
Sbjct: 361 SAGMIPDEFTMVSVLTACAHLGSLEIGEWIKTYIDKNKIKNDVVVGNALIDMYFKCGCSE 420

Query: 124 NALQVFEKMPSRGLSSWNSMIFGLAVNGCENEAILLFRMLESSSLEPDSVT 175
            A +VF  M  R   +W +M+ GLA NG   EAI +F  ++  S++PD +T
Sbjct: 421 KAQKVFHDMDQRDKFTWTAMVVGLANNGQGQEAIKVFFQMQDMSIQPDDIT 471

BLAST of MC04g0049 vs. ExPASy Swiss-Prot
Match: Q9FG16 (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 6.4e-38
Identity = 81/223 (36.32%), Postives = 131/223 (58.74%), Query Frame = 0

Query: 4   DAVAWNSMIMGVAKCGEIGESRKLFDTMPERNPISWNSMIGGYVRNGMFKEALKLFVKMQ 63
           D V+W SM+ G  KCG +  +R++FD MP RN  +W+ MI GY +N  F++A+ LF  M+
Sbjct: 182 DVVSWTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMK 241

Query: 64  EEKIKPSEFTMVSLLNASAHIGALRQGEWIHEYIEKNNLELNPIVVTAIIDMYCKCGSIV 123
            E +  +E  MVS++++ AH+GAL  GE  +EY+ K+++ +N I+ TA++DM+ +CG I 
Sbjct: 242 REGVVANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIE 301

Query: 124 NALQVFEKMPSRGLSSWNSMIFGLAVNGCENEAILLFRMLESSSLEPDSVTMEERVSMRM 183
            A+ VFE +P     SW+S+I GLAV+G  ++A+  F  + S    P  VT    +S   
Sbjct: 302 KAIHVFEGLPETDSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACS 361

Query: 184 KNVEKEPGGSLIEVDGEVHEFIAGGGRLHPKAQEIYIVLDQLG 227
                   G L+E   E++E +     + P+ +    ++D LG
Sbjct: 362 H-------GGLVEKGLEIYENMKKDHGIEPRLEHYGCIVDMLG 397

BLAST of MC04g0049 vs. NCBI nr
Match: XP_022147962.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia])

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 231 MFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEP 290
           MFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEP
Sbjct: 1   MFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEP 60

Query: 291 SKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEE 350
           SKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEE
Sbjct: 61  SKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEE 120

Query: 351 QLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFK 410
           QLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFK
Sbjct: 121 QLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFK 180

Query: 411 VVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEM 470
           VVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEM
Sbjct: 181 VVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEM 240

Query: 471 DKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILS 530
           DKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILS
Sbjct: 241 DKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILS 300

Query: 531 IGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHL 590
           IGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHL
Sbjct: 301 IGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHL 360

Query: 591 MNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARAD 650
           MNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARAD
Sbjct: 361 MNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARAD 420

Query: 651 RKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRDVKRERSNNSKGYQVRTKV 707
           RKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRDVKRERSNNSKGYQVRTKV
Sbjct: 421 RKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRDVKRERSNNSKGYQVRTKV 477

BLAST of MC04g0049 vs. NCBI nr
Match: KAG7012049.1 (Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 814 bits (2103), Expect = 1.32e-285
Identity = 471/802 (58.73%), Postives = 546/802 (68.08%), Query Frame = 0

Query: 19  GEIGESRKLFDTMPERNPISWNSMIGGYVRNGMFKEALKLFVKMQEEKIKPSEFTMVSLL 78
           G++    KLFD MP RNPISWNSMIGGYVRNGMFKEALKLFVKMQEE I+PSEF+MVSLL
Sbjct: 42  GKLVSRGKLFDKMPVRNPISWNSMIGGYVRNGMFKEALKLFVKMQEESIQPSEFSMVSLL 101

Query: 79  NASAHIGALRQGEWIHEYIEKNNLELNPIVVTAIID------------------------ 138
           NAS  IGAL      +EY++K NLEL+  V TAIID                        
Sbjct: 102 NASTQIGALSARRMENEYVKKKNLELSANVATAIIDIEWLRKGSSLKPDSVSFMENEGSG 161

Query: 139 -----------------------MYCKCGSIVNALQVFEKMPS------RGLSSW----N 198
                                  M+ + G +  A Q+ + MP       RG++      N
Sbjct: 162 FFSPTKNTYRIDASTKHYNLTVDMFSRAGFLEEAEQLLKTMPIKADAIIRGVTVCRTYGN 221

Query: 199 SMIFGLAVNGCENEAILLFRMLE-------------SSSLEPDSVTME---ERVSMRMKN 258
           + +   A        + L+RM               + ++ PD +  +    R +     
Sbjct: 222 TEMVKRAAEKVNEWVMFLWRMFMLDDEGLTEAQAEFTGTIRPDILAQKLPWARTASNQSQ 281

Query: 259 VEKEPG----GSLIEVDGEV--------------HEFIAGG------GRLHPKAQEIYIV 318
            +  P       L+ V   +                F+A         R  P  Q   + 
Sbjct: 282 RQSSPSFVLFSRLVAVPLALVRCRLLFSRSPLDSPSFLASSYQTSTFSRPSPSPQNFLLF 341

Query: 319 LDQLGERCTMFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMN 378
                   T      ++ +QQ H+F + +RTYVDGTIKWVRDRGLDHAVEREKNLLPVMN
Sbjct: 342 FLFRHPPSTASAGPPHKCRQQSHVFQISLRTYVDGTIKWVRDRGLDHAVEREKNLLPVMN 401

Query: 379 IKDFIKSEPSKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTS 438
           IK+FIKSEPSKSVPVSIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTS
Sbjct: 402 IKNFIKSEPSKSVPVSIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTS 461

Query: 439 QVLELDAEEQLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSI 498
           QVLELDAEEQL+YQ E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSI
Sbjct: 462 QVLELDAEEQLTYQSEICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSI 521

Query: 499 VPDFPDYFKVVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISF 558
           VP+FPDYFKVVGHQNFASG G  +ALELVCW+NELATSVLEKMAAK + G  SKG+ ++F
Sbjct: 522 VPEFPDYFKVVGHQNFASGWGTMQALELVCWSNELATSVLEKMAAKGKPGSTSKGIHVTF 581

Query: 559 PMKFSNGFEMDKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIA 618
           PMK+SNGFEMDKKFKKWVDEWQKLPYISPYENA+HL P+SDESDKW+VAILHE LHML+ 
Sbjct: 582 PMKYSNGFEMDKKFKKWVDEWQKLPYISPYENATHLPPNSDESDKWSVAILHEFLHMLVT 641

Query: 619 KKIEKETILSIGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPL 678
           KK EKET+L IGEYFGLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL
Sbjct: 642 KKTEKETVLGIGEYFGLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPL 701

Query: 679 INIRNKYLHLMNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAED 707
           + IRNKYLHLMNTVKEDSKTT+E   T QQK+E+KE SDD + K+NEAEL +S DE+ ++
Sbjct: 702 MIIRNKYLHLMNTVKEDSKTTSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSDDEDEDE 761

BLAST of MC04g0049 vs. NCBI nr
Match: XP_022952527.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 [Cucurbita moschata])

HSP 1 Score: 727 bits (1877), Expect = 1.03e-256
Identity = 380/483 (78.67%), Postives = 422/483 (87.37%), Query Frame = 0

Query: 237 NQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPV 296
           ++ +QQ H+F + +RTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIK+FIKSEPSKSVPV
Sbjct: 13  HKCRQQSHVFQISLRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKNFIKSEPSKSVPV 72

Query: 297 SIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQG 356
           SIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTSQVLELDAEEQL+YQ 
Sbjct: 73  SIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLTYQS 132

Query: 357 ENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQN 416
           E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSIVP+FPDYFKVVGHQN
Sbjct: 133 EICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSIVPEFPDYFKVVGHQN 192

Query: 417 FASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFK 476
           FASG G  +ALELVCWNNELATSVLEKMAAK + G  SKGMPI+FPMK+SNGFEMDKKFK
Sbjct: 193 FASGWGTMQALELVCWNNELATSVLEKMAAKGKPGSTSKGMPITFPMKYSNGFEMDKKFK 252

Query: 477 KWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYF 536
           KWVDEWQKLPYISPY NA+HL P+SDESDKW+VAILHE LHML+ KK EKET+L IGEYF
Sbjct: 253 KWVDEWQKLPYISPYINATHLPPNSDESDKWSVAILHEFLHMLVTKKTEKETVLGIGEYF 312

Query: 537 GLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVK 596
           GLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL+ IRNKYLHLMNTVK
Sbjct: 313 GLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPLMIIRNKYLHLMNTVK 372

Query: 597 EDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDD---------SSNLN 656
           EDSKTT+E   T QQK+E+KE SDD + K+NEAEL +S DE+ +++         SSN N
Sbjct: 373 EDSKTTSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSDDEDEDEEEDQGEDENASSNQN 432

Query: 657 ARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRD--VKRERSNNSKGYQVR 707
            +ADRKE  G  +RHVD +EN  R+R+RSSKR N ++ RPSRD   KRERSN SK   VR
Sbjct: 433 IQADRKED-GRNKRHVDLKENSVRDRMRSSKRRNLSAKRPSRDRDAKRERSNMSKRSSVR 492

BLAST of MC04g0049 vs. NCBI nr
Match: XP_023554109.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 [Cucurbita pepo subsp. pepo] >XP_023554110.1 protein ROOT PRIMORDIUM DEFECTIVE 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724 bits (1870), Expect = 1.84e-255
Identity = 382/495 (77.17%), Postives = 423/495 (85.45%), Query Frame = 0

Query: 237 NQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPV 296
           ++ +QQ H+F + +RTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIK+FIKSEPSKSVPV
Sbjct: 13  HKCRQQSHVFQISLRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKNFIKSEPSKSVPV 72

Query: 297 SIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQG 356
           SIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTSQVLELDAEEQL+YQ 
Sbjct: 73  SIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLTYQS 132

Query: 357 ENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQN 416
           E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSIVP+FPDYFKVVGHQN
Sbjct: 133 EICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSIVPEFPDYFKVVGHQN 192

Query: 417 FASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFK 476
           FASG G  +ALELVCW+NELATSVLEKMAAK + G  SKGM ++FPMK+SNGFEMDKKFK
Sbjct: 193 FASGWGTMQALELVCWSNELATSVLEKMAAKGKPGSTSKGMHVTFPMKYSNGFEMDKKFK 252

Query: 477 KWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYF 536
           KWVDEWQKLPYISPYENA+HL P+SDESDKW+VAILHE LHMLI KK EKET+L IGEYF
Sbjct: 253 KWVDEWQKLPYISPYENATHLPPNSDESDKWSVAILHEFLHMLITKKTEKETVLGIGEYF 312

Query: 537 GLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVK 596
           GLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL+ IRNKYLHLMNTVK
Sbjct: 313 GLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPLMIIRNKYLHLMNTVK 372

Query: 597 EDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEA------------EDDSS 656
           ED KTT+E   T QQK+E+KE SDD + K+NEAEL +SSD+E             ED SS
Sbjct: 373 EDGKTTSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSSDDEDDEEDEEEDQGEDEDASS 432

Query: 657 NLNARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRD-----------VKR 707
           N N +ADRKE  G  +R+VD +EN  R+R+RSSKR NS++ RPSRD            KR
Sbjct: 433 NHNIQADRKED-GPSKRYVDLKENAVRDRMRSSKRKNSSAKRPSRDRDAKRPSRDRDAKR 492

BLAST of MC04g0049 vs. NCBI nr
Match: XP_022969363.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X4 [Cucurbita maxima])

HSP 1 Score: 721 bits (1860), Expect = 3.91e-254
Identity = 381/483 (78.88%), Postives = 420/483 (86.96%), Query Frame = 0

Query: 237 NQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPV 296
           ++ +QQ H+F + +RTY DGTIKWVRDRGLDHAVEREKNLLPVMNIK+FIKSEPSKSVPV
Sbjct: 13  HKCRQQSHVFQISLRTYADGTIKWVRDRGLDHAVEREKNLLPVMNIKNFIKSEPSKSVPV 72

Query: 297 SIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQG 356
           SIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTSQVLELDAEEQL+ Q 
Sbjct: 73  SIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLTNQS 132

Query: 357 ENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQN 416
           E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSIVP+FPDYFKVVGHQN
Sbjct: 133 EICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSIVPEFPDYFKVVGHQN 192

Query: 417 FASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFK 476
           FASG G  +ALELVCWNNELATSVLEKMAAK + G  SKGM I+FPM +SNGFEMDKKFK
Sbjct: 193 FASGWGTKQALELVCWNNELATSVLEKMAAKGKPGSTSKGMHITFPMNYSNGFEMDKKFK 252

Query: 477 KWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYF 536
           KWVDEWQKLPYISPYE+A+HL PSSDESDKW+VAILHE LHML+ KK EKET+L IGEYF
Sbjct: 253 KWVDEWQKLPYISPYESATHLPPSSDESDKWSVAILHEFLHMLVTKKTEKETVLGIGEYF 312

Query: 537 GLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVK 596
           GLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL+ IRNKYLHLMNTVK
Sbjct: 313 GLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPLMIIRNKYLHLMNTVK 372

Query: 597 EDSKTTT-EQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDD--------SSNLN 656
           EDSKTT+ E   T QQK+E+KE SDD + K+NEAEL +SS  E ED+        SSN N
Sbjct: 373 EDSKTTSSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSSANEDEDEDEGEGEDASSNHN 432

Query: 657 ARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRD--VKRERSNNSKGYQVR 707
            +ADRKE  G  +RHVD +EN  R+R+RSSKR NS++ RPSRD   KRERSN SK Y VR
Sbjct: 433 IQADRKED-GRSKRHVDLKENAVRDRMRSSKRKNSSAKRPSRDRDAKRERSNMSKRYSVR 492

BLAST of MC04g0049 vs. ExPASy TrEMBL
Match: A0A6J1D3S2 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC111016760 PE=4 SV=1)

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 231 MFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEP 290
           MFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEP
Sbjct: 1   MFLLSRNQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEP 60

Query: 291 SKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEE 350
           SKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEE
Sbjct: 61  SKSVPVSIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEE 120

Query: 351 QLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFK 410
           QLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFK
Sbjct: 121 QLSYQGENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFK 180

Query: 411 VVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEM 470
           VVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEM
Sbjct: 181 VVGHQNFASGSGDTRALELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEM 240

Query: 471 DKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILS 530
           DKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILS
Sbjct: 241 DKKFKKWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILS 300

Query: 531 IGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHL 590
           IGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHL
Sbjct: 301 IGEYFGLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHL 360

Query: 591 MNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARAD 650
           MNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARAD
Sbjct: 361 MNTVKEDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARAD 420

Query: 651 RKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRDVKRERSNNSKGYQVRTKV 707
           RKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRDVKRERSNNSKGYQVRTKV
Sbjct: 421 RKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRDVKRERSNNSKGYQVRTKV 477

BLAST of MC04g0049 vs. ExPASy TrEMBL
Match: A0A6J1GKN4 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucurbita moschata OX=3662 GN=LOC111455191 PE=4 SV=1)

HSP 1 Score: 727 bits (1877), Expect = 4.98e-257
Identity = 380/483 (78.67%), Postives = 422/483 (87.37%), Query Frame = 0

Query: 237 NQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPV 296
           ++ +QQ H+F + +RTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIK+FIKSEPSKSVPV
Sbjct: 13  HKCRQQSHVFQISLRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKNFIKSEPSKSVPV 72

Query: 297 SIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQG 356
           SIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTSQVLELDAEEQL+YQ 
Sbjct: 73  SIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLTYQS 132

Query: 357 ENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQN 416
           E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSIVP+FPDYFKVVGHQN
Sbjct: 133 EICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSIVPEFPDYFKVVGHQN 192

Query: 417 FASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFK 476
           FASG G  +ALELVCWNNELATSVLEKMAAK + G  SKGMPI+FPMK+SNGFEMDKKFK
Sbjct: 193 FASGWGTMQALELVCWNNELATSVLEKMAAKGKPGSTSKGMPITFPMKYSNGFEMDKKFK 252

Query: 477 KWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYF 536
           KWVDEWQKLPYISPY NA+HL P+SDESDKW+VAILHE LHML+ KK EKET+L IGEYF
Sbjct: 253 KWVDEWQKLPYISPYINATHLPPNSDESDKWSVAILHEFLHMLVTKKTEKETVLGIGEYF 312

Query: 537 GLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVK 596
           GLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL+ IRNKYLHLMNTVK
Sbjct: 313 GLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPLMIIRNKYLHLMNTVK 372

Query: 597 EDSKTTTEQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDD---------SSNLN 656
           EDSKTT+E   T QQK+E+KE SDD + K+NEAEL +S DE+ +++         SSN N
Sbjct: 373 EDSKTTSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSDDEDEDEEEDQGEDENASSNQN 432

Query: 657 ARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRD--VKRERSNNSKGYQVR 707
            +ADRKE  G  +RHVD +EN  R+R+RSSKR N ++ RPSRD   KRERSN SK   VR
Sbjct: 433 IQADRKED-GRNKRHVDLKENSVRDRMRSSKRRNLSAKRPSRDRDAKRERSNMSKRSSVR 492

BLAST of MC04g0049 vs. ExPASy TrEMBL
Match: A0A6J1I0R6 (protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111468390 PE=4 SV=1)

HSP 1 Score: 721 bits (1860), Expect = 1.89e-254
Identity = 381/483 (78.88%), Postives = 420/483 (86.96%), Query Frame = 0

Query: 237 NQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPV 296
           ++ +QQ H+F + +RTY DGTIKWVRDRGLDHAVEREKNLLPVMNIK+FIKSEPSKSVPV
Sbjct: 13  HKCRQQSHVFQISLRTYADGTIKWVRDRGLDHAVEREKNLLPVMNIKNFIKSEPSKSVPV 72

Query: 297 SIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQG 356
           SIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTSQVLELDAEEQL+ Q 
Sbjct: 73  SIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLTNQS 132

Query: 357 ENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQN 416
           E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSIVP+FPDYFKVVGHQN
Sbjct: 133 EICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSIVPEFPDYFKVVGHQN 192

Query: 417 FASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFK 476
           FASG G  +ALELVCWNNELATSVLEKMAAK + G  SKGM I+FPM +SNGFEMDKKFK
Sbjct: 193 FASGWGTKQALELVCWNNELATSVLEKMAAKGKPGSTSKGMHITFPMNYSNGFEMDKKFK 252

Query: 477 KWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYF 536
           KWVDEWQKLPYISPYE+A+HL PSSDESDKW+VAILHE LHML+ KK EKET+L IGEYF
Sbjct: 253 KWVDEWQKLPYISPYESATHLPPSSDESDKWSVAILHEFLHMLVTKKTEKETVLGIGEYF 312

Query: 537 GLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVK 596
           GLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL+ IRNKYLHLMNTVK
Sbjct: 313 GLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPLMIIRNKYLHLMNTVK 372

Query: 597 EDSKTTT-EQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDD--------SSNLN 656
           EDSKTT+ E   T QQK+E+KE SDD + K+NEAEL +SS  E ED+        SSN N
Sbjct: 373 EDSKTTSSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSSANEDEDEDEGEGEDASSNHN 432

Query: 657 ARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRD--VKRERSNNSKGYQVR 707
            +ADRKE  G  +RHVD +EN  R+R+RSSKR NS++ RPSRD   KRERSN SK Y VR
Sbjct: 433 IQADRKED-GRSKRHVDLKENAVRDRMRSSKRKNSSAKRPSRDRDAKRERSNMSKRYSVR 492

BLAST of MC04g0049 vs. ExPASy TrEMBL
Match: A0A6J1HW55 (protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111468390 PE=4 SV=1)

HSP 1 Score: 719 bits (1856), Expect = 8.88e-254
Identity = 381/487 (78.23%), Postives = 420/487 (86.24%), Query Frame = 0

Query: 237 NQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPV 296
           ++ +QQ H+F + +RTY DGTIKWVRDRGLDHAVEREKNLLPVMNIK+FIKSEPSKSVPV
Sbjct: 13  HKCRQQSHVFQISLRTYADGTIKWVRDRGLDHAVEREKNLLPVMNIKNFIKSEPSKSVPV 72

Query: 297 SIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQG 356
           SIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTSQVLELDAEEQL+ Q 
Sbjct: 73  SIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLTNQS 132

Query: 357 ENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQN 416
           E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSIVP+FPDYFKVVGHQN
Sbjct: 133 EICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSIVPEFPDYFKVVGHQN 192

Query: 417 FASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFK 476
           FASG G  +ALELVCWNNELATSVLEKMAAK + G  SKGM I+FPM +SNGFEMDKKFK
Sbjct: 193 FASGWGTKQALELVCWNNELATSVLEKMAAKGKPGSTSKGMHITFPMNYSNGFEMDKKFK 252

Query: 477 KWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYF 536
           KWVDEWQKLPYISPYE+A+HL PSSDESDKW+VAILHE LHML+ KK EKET+L IGEYF
Sbjct: 253 KWVDEWQKLPYISPYESATHLPPSSDESDKWSVAILHEFLHMLVTKKTEKETVLGIGEYF 312

Query: 537 GLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVK 596
           GLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL+ IRNKYLHLMNTVK
Sbjct: 313 GLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPLMIIRNKYLHLMNTVK 372

Query: 597 EDSKTTT-EQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDD------------S 656
           EDSKTT+ E   T QQK+E+KE SDD + K+NEAEL +SS  E ED+            S
Sbjct: 373 EDSKTTSSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSSANEDEDEDEDEDEGEGEDAS 432

Query: 657 SNLNARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRD--VKRERSNNSKG 707
           SN N +ADRKE  G  +RHVD +EN  R+R+RSSKR NS++ RPSRD   KRERSN SK 
Sbjct: 433 SNHNIQADRKED-GRSKRHVDLKENAVRDRMRSSKRKNSSAKRPSRDRDAKRERSNMSKR 492

BLAST of MC04g0049 vs. ExPASy TrEMBL
Match: A0A6J1HXK3 (protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468390 PE=4 SV=1)

HSP 1 Score: 717 bits (1850), Expect = 9.00e-253
Identity = 381/493 (77.28%), Postives = 420/493 (85.19%), Query Frame = 0

Query: 237 NQSQQQHHLFHLYIRTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPV 296
           ++ +QQ H+F + +RTY DGTIKWVRDRGLDHAVEREKNLLPVMNIK+FIKSEPSKSVPV
Sbjct: 13  HKCRQQSHVFQISLRTYADGTIKWVRDRGLDHAVEREKNLLPVMNIKNFIKSEPSKSVPV 72

Query: 297 SIITQKREVLRIPTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQG 356
           SIITQKREVLRIPTRPIDLIRKYP+IFEEFLPGGIGIQPHVRLTSQVLELDAEEQL+ Q 
Sbjct: 73  SIITQKREVLRIPTRPIDLIRKYPTIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLTNQS 132

Query: 357 ENCRQRAADQLVKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQN 416
           E CRQ+AAD+LVKLLM+SR HKIPLSIIDQLKW+LGLPKDYVQSIVP+FPDYFKVVGHQN
Sbjct: 133 EICRQQAADRLVKLLMLSRVHKIPLSIIDQLKWELGLPKDYVQSIVPEFPDYFKVVGHQN 192

Query: 417 FASGSGDTRALELVCWNNELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFK 476
           FASG G  +ALELVCWNNELATSVLEKMAAK + G  SKGM I+FPM +SNGFEMDKKFK
Sbjct: 193 FASGWGTKQALELVCWNNELATSVLEKMAAKGKPGSTSKGMHITFPMNYSNGFEMDKKFK 252

Query: 477 KWVDEWQKLPYISPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYF 536
           KWVDEWQKLPYISPYE+A+HL PSSDESDKW+VAILHE LHML+ KK EKET+L IGEYF
Sbjct: 253 KWVDEWQKLPYISPYESATHLPPSSDESDKWSVAILHEFLHMLVTKKTEKETVLGIGEYF 312

Query: 537 GLRSRFKRALLHHPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVK 596
           GLRSRFKRALLHHPGIFYLSSK GTYTVVLKE YKRGSVIE+SPL+ IRNKYLHLMNTVK
Sbjct: 313 GLRSRFKRALLHHPGIFYLSSKAGTYTVVLKEAYKRGSVIESSPLMIIRNKYLHLMNTVK 372

Query: 597 EDSKTTT-EQGGTCQQKKEEKERSDDATRKENEAELLDSSDEEAEDD------------- 656
           EDSKTT+ E   T QQK+E+KE SDD + K+NEAEL +SS  E ED+             
Sbjct: 373 EDSKTTSSEHKSTRQQKQEQKEGSDDGSGKQNEAELFNSSANEDEDEDEDEDEDEDEDEG 432

Query: 657 -----SSNLNARADRKEAAGGRRRHVDFQENVGRNRVRSSKRMNSTSGRPSRD--VKRER 707
                SSN N +ADRKE  G  +RHVD +EN  R+R+RSSKR NS++ RPSRD   KRER
Sbjct: 433 EGEDASSNHNIQADRKED-GRSKRHVDLKENAVRDRMRSSKRKNSSAKRPSRDRDAKRER 492

BLAST of MC04g0049 vs. TAIR 10
Match: AT1G71850.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 451.1 bits (1159), Expect = 1.7e-126
Identity = 242/447 (54.14%), Postives = 317/447 (70.92%), Query Frame = 0

Query: 254 VDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPVSIITQKREVLRIPTRPI 313
           V+ T K+VRDRGLDHAVEREKNL P+++IKD I+SEP+KSVP+S+IT +++ LR+P RPI
Sbjct: 25  VEDTYKFVRDRGLDHAVEREKNLRPLLSIKDLIRSEPAKSVPISVITSQKDSLRVPLRPI 84

Query: 314 DLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQGENCRQRAADQLVKLLMI 373
           + IR +PS+F+EFLPGGIGI PH+ LT ++L  DA+EQL Y  E  +Q  AD+L+KLLMI
Sbjct: 85  EFIRSFPSVFQEFLPGGIGIHPHISLTPEILNHDADEQLVYGSETYKQGLADRLLKLLMI 144

Query: 374 SRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQNFASGSGDTRALELVCWN 433
           +R +KIPL I+D LKWDLGLPKDYV+++VP+FPDYF+V+        SG+   LELVCW+
Sbjct: 145 NRINKIPLEILDLLKWDLGLPKDYVETMVPEFPDYFRVI-KSKLRGCSGE---LELVCWS 204

Query: 434 NELATSVLEKMAAKAETG-VSKGMPISFPMKFSNGFEMDKKFKKWVDEWQKLPYISPYEN 493
           NE A SVLEK A     G  +KG  I+FPMKFSNGF +DKK KKW+D+WQKLPYISPYEN
Sbjct: 205 NEHAVSVLEKKARTLRKGEYTKGSAIAFPMKFSNGFVVDKKMKKWIDDWQKLPYISPYEN 264

Query: 494 ASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYFGLRSRFKRALLHHPGIF 553
           A HLS +SDESDKWA A+LHE++++ ++KK+EK+ IL +GE+ GLRSRFKR L +HPGIF
Sbjct: 265 ALHLSATSDESDKWAAAVLHEIMNLFVSKKVEKDAILHLGEFMGLRSRFKRVLHNHPGIF 324

Query: 554 YLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMNTVKEDSKTTTEQGGTCQQKK 613
           YLSSK  T+TVVL++GYKRG +IE++ L+  RN+Y+ LMNTVK+D+K  +       + K
Sbjct: 325 YLSSKLRTHTVVLRDGYKRGMLIESNELVTSRNRYMKLMNTVKKDNKAVSSSSKKEDKGK 384

Query: 614 EEKERSDDATRKENEAELLDSSDEEAEDDSSNLNARADRKEAAGGRRRHVDFQENVGRNR 673
            E E  D   + EN+    D S  + EDD        D  +        VD  +++ R R
Sbjct: 385 VEGEVCDTDAKAEND----DISGSDVEDDRQGDFVDDDEDD------DEVDQNQDLERGR 444

Query: 674 VRSSKRMNSTSGRPSRDVKRERSNNSK 700
             SS R    S   S    + +S  SK
Sbjct: 445 RNSSPRSGRRSFGNSGSRDKAQSRRSK 457

BLAST of MC04g0049 vs. TAIR 10
Match: AT4G24320.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 298.5 bits (763), Expect = 1.4e-80
Identity = 149/343 (43.44%), Postives = 223/343 (65.01%), Query Frame = 0

Query: 251 RTYVDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPVSIITQKREVLRIPT 310
           RT+V+  +KWV D  LD AV+REKNL  V+++KD I S PSKS+P+S ++  + ++ +  
Sbjct: 16  RTFVNARVKWVSDHYLDEAVQREKNLKQVISLKDRIVSSPSKSLPLSSLSLLKPLVNLHI 75

Query: 311 RPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQGENCRQRAADQLVKL 370
                 +KYPS+F  F P       HVRLT Q L L  EE+  +     R     +L K 
Sbjct: 76  TAAAFFQKYPSVFTTFQPSP-SHPLHVRLTPQALTLHKEEETIHLSPPQRNVTVQRLTKF 135

Query: 371 LMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQNFASGSGDTRALELV 430
           LM++    +PL ++D+ ++DLGLP+DY+ S++ D+P+YF+V   ++  +G   T AL + 
Sbjct: 136 LMLTGAGSLPLYVLDRFRFDLGLPRDYITSLIGDYPEYFEVTEIKDRLTGE-KTLALTIS 195

Query: 431 CWNNELATSVLEKMAAKAE-TGVSKGMPISFPMKFSNGFEMDKKFKKWVDEWQKLPYISP 490
              N L  S +E+  A  + + V KG+ I + M F  G+E+ K+ K WV++WQ LPYISP
Sbjct: 196 SRRNNLPVSEMERREATIDGSRVKKGLRIRYSMNFPKGYELHKRVKNWVEQWQNLPYISP 255

Query: 491 YENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYFGLRSRFKRALLHHP 550
           YENA HL   SD+++KWAVA+LHELL +L++KK E + ++ +GEY G   RFK+AL+HHP
Sbjct: 256 YENAFHLGSYSDQAEKWAVAVLHELLFLLVSKKTETDNVICLGEYLGFGMRFKKALVHHP 315

Query: 551 GIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMN 593
           GIFY+S K  T TVVL+E Y +  ++E  PL+ IR++Y++LM+
Sbjct: 316 GIFYMSHKIRTQTVVLREAYHKVFLLEKHPLMGIRHQYIYLMS 356

BLAST of MC04g0049 vs. TAIR 10
Match: AT2G42920.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 7.3e-61
Identity = 112/172 (65.12%), Postives = 130/172 (75.58%), Query Frame = 0

Query: 3   FDAVAWNSMIMGVAKCGEIGESRKLFDTMPERNPISWNSMIGGYVRNGMFKEALKLFVKM 62
           FD VAWNSMIMG AKCG I +++ LFD MP+RN +SWNSMI G+VRNG FK+AL +F +M
Sbjct: 190 FDVVAWNSMIMGFAKCGLIDQAQNLFDEMPQRNGVSWNSMISGFVRNGRFKDALDMFREM 249

Query: 63  QEEKIKPSEFTMVSLLNASAHIGALRQGEWIHEYIEKNNLELNPIVVTAIIDMYCKCGSI 122
           QE+ +KP  FTMVSLLNA A++GA  QG WIHEYI +N  ELN IVVTA+IDMYCKCG I
Sbjct: 250 QEKDVKPDGFTMVSLLNACAYLGASEQGRWIHEYIVRNRFELNSIVVTALIDMYCKCGCI 309

Query: 123 VNALQVFEKMPSRGLSSWNSMIFGLAVNGCENEAILLFRMLESSSLEPDSVT 175
              L VFE  P + LS WNSMI GLA NG E  A+ LF  LE S LEPDSV+
Sbjct: 310 EEGLNVFECAPKKQLSCWNSMILGLANNGFEERAMDLFSELERSGLEPDSVS 361


HSP 2 Score: 53.9 bits (128), Expect = 6.0e-07
Identity = 34/69 (49.28%), Postives = 45/69 (65.22%), Query Frame = 0

Query: 157 ILLFRMLESSSLEPDSVTMEERVSMRMKNVEKEPGGSLIEVDGEVHEFIAGGGRLHPKAQ 216
           +LL     S  L  ++V  E+R+ M+ + +EKE G S IEVD EVHEFI+ GG  HPK+ 
Sbjct: 465 VLLSNAYASYGLFEEAV--EQRLLMKERQMEKEVGCSSIEVDFEVHEFISCGG-THPKSA 524

Query: 217 EIYIVLDQL 226
           EIY +LD L
Sbjct: 525 EIYSLLDIL 530

BLAST of MC04g0049 vs. TAIR 10
Match: AT2G39120.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 221.1 bits (562), Expect = 2.9e-57
Identity = 127/343 (37.03%), Postives = 194/343 (56.56%), Query Frame = 0

Query: 251 RTYVDGTIKWVRDRGLDHA--VEREKNLLPVMNIKDFIKSEPSKSVPVSIITQKREVLRI 310
           RTYVD  +KW RD   D+   + R   L  V+++K+ I  EP++ +P+S I++K     +
Sbjct: 22  RTYVDVYMKWKRDPYFDNIEHILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTRQFDV 81

Query: 311 PTRPIDLIRKYPSIFEEFLPGGIGIQPHVRLTSQVLELDAEEQLSYQGENCRQRAADQLV 370
            T+    +RK+PSIFEEF+     + P  RLT +  ELD +E++ YQ         D+L 
Sbjct: 82  STKIAHFLRKFPSIFEEFVGPEYNL-PWFRLTPEATELDRQERVVYQ--TSADDLRDRLK 141

Query: 371 KLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQNFASGSGDTRALE 430
           KL+++S+ + +PLSI+  +KW LGLP DY+Q     FPD   +     F       + L 
Sbjct: 142 KLILMSKDNVLPLSIVQGMKWYLGLPDDYLQ-----FPD-MNLDSSFRFVDMEDGVKGLA 201

Query: 431 LVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEMDKKFKKWVDEWQKLPYIS 490
           +     +   SVL+K A K   G      I FP+  S G  +  K + W+ E+QKLPY+S
Sbjct: 202 VDYNGGDKVLSVLQKNAMKKRRGEVSLEEIEFPLFPSKGCRLRVKIEDWLMEFQKLPYVS 261

Query: 491 PYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYFGLRSRFKRALLHH 550
           PY++ S L PSSD ++K  V  LHELL + +    E++ +L + ++FGL  +  +A   H
Sbjct: 262 PYDDYSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKAFERH 321

Query: 551 PGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLM 592
           P IFYLS KN T T +L+E Y+  + +E  P++ +R KY+ LM
Sbjct: 322 PQIFYLSMKNKTCTAILREPYRDKASVETHPVLGVRKKYIQLM 355

BLAST of MC04g0049 vs. TAIR 10
Match: AT5G48040.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 220.7 bits (561), Expect = 3.7e-57
Identity = 131/345 (37.97%), Postives = 195/345 (56.52%), Query Frame = 0

Query: 254 VDGTIKWVRDRGLDHAVEREKNLLPVMNIKDFIKSEPSKSVPVSIITQKREVLRIP--TR 313
           V+  +KWV+DR LD  V REK+L  V N+   I + P   +P+  +   R  L +P   +
Sbjct: 33  VNVKLKWVKDRELDAVVVREKHLRAVCNLVSVISASPDLRLPIFKLLPHRGQLGLPQELK 92

Query: 314 PIDLIRKYPSIFEEFL---PGGIGIQPHVRLTSQVLELDAEE-QLSYQGENCRQRAADQL 373
               IR+YP+IF E       G  + P   LT + ++L  EE  +S   E   +    +L
Sbjct: 93  LSAFIRRYPNIFVEHCYWDSAGTSV-PCFGLTRETIDLYYEEVDVSRVNE---RDVLVRL 152

Query: 374 VKLLMISRFHKIPLSIIDQLKWDLGLPKDYVQSIVPDFPDYFKVVGHQNFASGSGDTRAL 433
            KLLM++    + L  ID L+WDLGLP DY  S++   PD F +V        S D   L
Sbjct: 153 CKLLMLTCERTLSLHSIDHLRWDLGLPYDYRDSLITKHPDLFSLV------KLSSDLDGL 212

Query: 434 ELVCWNNELATSVLEKMAAKAETGVSKGMPISFPMKFSNGFEMDKKFKKWVDEWQKLPYI 493
           +L+ W+  LA S   +M  + + G  + M  +FP+KF+ GF + +K  +W+ EWQ+LPY 
Sbjct: 213 KLIHWDEHLAVS---QMQLREDVGNDERM--AFPVKFTRGFGLKRKSIEWLQEWQRLPYT 272

Query: 494 SPYENASHLSPSSDESDKWAVAILHELLHMLIAKKIEKETILSIGEYFGLRSRFKRALLH 553
           SPY +ASHL P +D S+K  V + HELLH+ I KK E++ + ++ + F L  +F +    
Sbjct: 273 SPYVDASHLDPRTDLSEKRNVGVFHELLHLTIGKKTERKNVSNLRKPFALPQKFTKVFER 332

Query: 554 HPGIFYLSSKNGTYTVVLKEGYKRGSVIENSPLINIRNKYLHLMN 593
           HPGIFY+S K  T TV+L+E Y R  +IE  PL+ +R K+ ++MN
Sbjct: 333 HPGIFYISMKCDTQTVILREAYDRRHLIEKHPLVEVREKFANMMN 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJG61.0e-5965.12Pentatricopeptide repeat-containing protein At2g42920, chloroplastic OS=Arabidop... [more]
Q9ZUZ64.0e-5637.03Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=W... [more]
Q389592.6e-3944.71Pentatricopeptide repeat-containing protein At3g26630, chloroplastic OS=Arabidop... [more]
Q9LSB86.4e-3844.44Putative pentatricopeptide repeat-containing protein At3g15930 OS=Arabidopsis th... [more]
Q9FG166.4e-3836.32Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_022147962.10.0100.00protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia][more]
KAG7012049.11.32e-28558.73Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita a... [more]
XP_022952527.11.03e-25678.67protein ROOT PRIMORDIUM DEFECTIVE 1 [Cucurbita moschata][more]
XP_023554109.11.84e-25577.17protein ROOT PRIMORDIUM DEFECTIVE 1 [Cucurbita pepo subsp. pepo] >XP_023554110.1... [more]
XP_022969363.13.91e-25478.88protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1D3S20.0100.00protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC1110167... [more]
A0A6J1GKN44.98e-25778.67protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucurbita moschata OX=3662 GN=LOC11145519... [more]
A0A6J1I0R61.89e-25478.88protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X4 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HW558.88e-25478.23protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HXK39.00e-25377.28protein ROOT PRIMORDIUM DEFECTIVE 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G71850.11.7e-12654.14Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT4G24320.11.4e-8043.44Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT2G42920.17.3e-6165.12Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT2G39120.12.9e-5737.03Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G48040.13.7e-5737.97Ubiquitin carboxyl-terminal hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 611..631
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 684..699
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 608..630
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 597..707
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 641..673
NoneNo IPR availablePANTHERPTHR31476:SF16F14O23.23 PROTEINcoord: 238..657
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 109..138
e-value: 6.0E-5
score: 21.0
coord: 139..172
e-value: 4.8E-4
score: 18.1
coord: 37..71
e-value: 7.2E-11
score: 39.6
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 139..166
e-value: 0.0013
score: 18.8
coord: 109..137
e-value: 4.1E-5
score: 23.5
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 35..80
e-value: 1.6E-12
score: 47.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 4..34
score: 9.678885
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 35..69
score: 13.67976
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 105..139
score: 10.632519
IPR021099Plant organelle RNA recognition domainPFAMPF11955PORRcoord: 261..595
e-value: 2.6E-115
score: 384.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 87..198
e-value: 6.6E-19
score: 70.4
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..86
e-value: 6.0E-24
score: 86.4
IPR045040PORR familyPANTHERPTHR31476PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTICcoord: 238..657

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0049.1MC04g0049.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0003723 RNA binding