MC03g_new0405 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0405 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 5.7e-153
Identity = 265/495 (53.54%), Postives = 349/495 (70.51%), Query Frame = 0

Query: 22  LCIFFFFLV-LLKLFKPKSNTR--NFPPSPPKLPIIGNLHQLGSLPHQSVAALSDKHGPL 81
           LC+  F L  L +   P    R    PPSPP+LP IGNLHQLGS PH+S+ ALS K+G +
Sbjct: 31  LCLSIFILFSLTRSSSPSEKNRKLKLPPSPPRLPWIGNLHQLGSFPHRSLRALSKKYGDV 90

Query: 82  MLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYWRQ 141
           M +  G+ PTL+VSSA+MA ++MKT D+ F +RPQT A  IL Y   D+ FAPYGEYWRQ
Sbjct: 91  MFMHFGKVPTLIVSSAEMAKDVMKTQDIVFCSRPQTTAPSILFYDGHDIAFAPYGEYWRQ 150

Query: 142 ARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGS--VNLSQLFLSTSNNIVS 201
            R+IC LEL S KRV  FQY R EEVA LV++IRK         +NL +L +STSNNI+ 
Sbjct: 151 VRRICVLELLSLKRVHQFQYARVEEVAELVSKIRKASASANGAPINLGELLVSTSNNIIC 210

Query: 202 RCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVRGFNGELKKWFTTL 261
           RCILG+KFED+   + FG+T++ +M  + +F F DFFP+L WID  RG+   LK  +   
Sbjct: 211 RCILGQKFEDKE-DNWFGETTKELMTQVMSFSFGDFFPSLKWIDRARGYLAYLKSIWLEF 270

Query: 262 DAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSLENLKGIVLDMFVG 321
           D F  K+++EHKA  +   E    KKD V I+L +     +D+  +  N+K I+ DMFVG
Sbjct: 271 DKFFDKLIDEHKAAQK---EGKPRKKDIVDILLDVQNDGSLDFELTTSNVKAILQDMFVG 330

Query: 322 GTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHYMNCVIKESLR 381
           G+DT+ T   W M+EL +NP VM+KVQEEVR+  GK+  +E  DI +M Y+ CVIKE+LR
Sbjct: 331 GSDTSWTAAIWLMSELSQNPRVMKKVQEEVRRVAGKRGYVEESDINEMKYLTCVIKENLR 390

Query: 382 LHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEFIPERFMENSV 441
           LHPP PLL+PREA+SDV++ G+ IP+KT+VFVNA+ + RDP +W++P+EF+PERF EN+V
Sbjct: 391 LHPPAPLLLPREAMSDVKLGGFDIPAKTQVFVNAYAVQRDPKVWDKPDEFMPERFEENNV 450

Query: 442 DYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGFE-----LDVEEE 501
            + GQDFE IPFG+GRR+CPG++FG+AS +Y LAN+LYWFDWKLP+G       LD+ E 
Sbjct: 451 GFVGQDFELIPFGAGRRVCPGLAFGVASAQYVLANMLYWFDWKLPSGGSKLAETLDMSEV 510

Query: 502 NGLTVRKKNPLHLNP 507
            GLTV KK+PL+L P
Sbjct: 511 YGLTVHKKSPLYLVP 521

BLAST of MC03g_new0405 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 533.5 bits (1373), Expect = 2.6e-150
Identity = 254/488 (52.05%), Postives = 347/488 (71.11%), Query Frame = 0

Query: 21  SLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLGSLPHQSVAALSDKHGPLML 80
           ++ + FF   LLKL + +    N PPSPP LPIIGNLHQLG+LPH+S+ +L+++ GPL+L
Sbjct: 11  AIALTFF---LLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLIL 70

Query: 81  LKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYWRQAR 140
           L LG  PTL+VS+A++A E++KTHDL F++RP T AA+ + Y   DV F+PYGEYWRQ R
Sbjct: 71  LHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVR 130

Query: 141 KICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNNIVSRCIL 200
           KIC LEL S KRV S++ +R+EEV +++ RI + C    +VNLS+L L  S+  ++R   
Sbjct: 131 KICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAF 190

Query: 201 GEKFE-DENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVRGFNGELKKWFTTLDAF 260
           G+K+E +E  K++F D +  +  L+ AF   D+FP+  W+DV+ G +  LK+    LDAF
Sbjct: 191 GKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAF 250

Query: 261 LSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSLENLKGIVLDMFVGGTD 320
           +  V+++H    +A G     +KD V ++L L +   +  H +  NLK ++LDMF GGTD
Sbjct: 251 VDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTD 310

Query: 321 TTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHYMNCVIKESLRLHP 380
           TTA  LEW M EL+++P VMEK Q+EVR+ VGKKAK+E ED+ ++HY+  +IKE+LRLHP
Sbjct: 311 TTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLHP 370

Query: 381 PVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEFIPERFMENSVDYK 440
             PLLVPRE+  DV I GYHIP+KTRVF+NAW I RDP  WE   EF+PERF+ NSVD+K
Sbjct: 371 VAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSVDFK 430

Query: 441 GQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGF---ELDVEEENGLTV 500
           GQDF+ IPFG+GRR CPG++FGI+S E +LANLLYWF+W+LP      +LD+ E  G+TV
Sbjct: 431 GQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVGITV 490

Query: 501 RKKNPLHL 505
             K PL L
Sbjct: 491 HMKFPLQL 495

BLAST of MC03g_new0405 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.9e-128
Identity = 240/513 (46.78%), Postives = 328/513 (63.94%), Query Frame = 0

Query: 4   MDPISKFQQLHFD---LIPWSLCIFFFFLVLLKLF----KPKSNTRNFPPSPPKLPIIGN 63
           ++P+   Q+L+     L P +  +   FLVLL  +    K  +     PPSPPKLPIIGN
Sbjct: 2   LNPLQVLQELNESSSFLQPLAFTLLAIFLVLLYTWYSSTKTTTQKSTQPPSPPKLPIIGN 61

Query: 64  LHQLGSLPHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVA 123
           LHQ+GS PH+S+ ALS +HGPLMLL  G  P LVVSSA+ A E++KTHDL FS+RP++  
Sbjct: 62  LHQIGSYPHRSLQALSQRHGPLMLLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTI 121

Query: 124 AKILLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCV 183
            + LLY +KDV  APYGEYWRQ R IC L L S +RV SF+ VR+EE   ++  I+    
Sbjct: 122 FEKLLYNYKDVASAPYGEYWRQVRSICVLNLLSNRRVRSFRSVREEETKSMIRNIKGS-- 181

Query: 184 GEGSVNLSQLFLSTSNNIVSRCILGEKFED-ENGKS--RFGDTSRRVMVLITAFCFADFF 243
               +NLS++F+  +N++V +  LG K+ D E G+S   F +       L+      D+ 
Sbjct: 182 SSSVLNLSEMFVRLTNDVVCKVALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYV 241

Query: 244 PALWWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQ 303
           P L W+  V G   +L K    LD F+  VV+EH       G+ D+  KDF+ I+L + +
Sbjct: 242 PWLSWLSHVNGLGAKLDKVAKQLDDFIDTVVQEHMNHSSRSGDDDQ--KDFLDILLAIQK 301

Query: 304 SDMVDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKK 363
                      ++KGI+LDMF  GTDTT + LEW MTEL+R+P VM K+Q EVR  VG +
Sbjct: 302 ETSAGIPIDGVSVKGIILDMFAAGTDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNR 361

Query: 364 AKIEIE-DIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWK 423
             +  E D+ +MHY+  V KE+LRLHPP+PLLVPR +  DVE+ GY+I + T+VF++AW+
Sbjct: 362 TDVITEDDLVEMHYLKAVTKETLRLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQ 421

Query: 424 IHRDPTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANL 483
           I RDP ++++P EF PERF+ N +DYKG DFE IPFG+GRR+CPG+ F +A  E ALAN+
Sbjct: 422 IGRDPKLYDKPEEFEPERFLNNGIDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANI 481

Query: 484 LYWFDWKLP---NGFELDVEEENGLTVRKKNPL 503
           ++ FDW LP   +G +LD+ E  GLT  KK PL
Sbjct: 482 VHKFDWALPDEASGEDLDMTETTGLTAHKKYPL 510

BLAST of MC03g_new0405 vs. ExPASy Swiss-Prot
Match: A0A0N9HTU1 (Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.1e-127
Identity = 225/490 (45.92%), Postives = 318/490 (64.90%), Query Frame = 0

Query: 22  LCIFFFFLVLLKLFKPK-SNTRNFPPSPPKLPIIGNLHQLGSLPHQSVAALSDKHGPLML 81
           L +F  F+  + + K K S+  N PPSPPKLPI+GN HQLG+L H++V  L+ K+GPLML
Sbjct: 7   LTLFLLFISTVFILKRKFSHKPNLPPSPPKLPILGNFHQLGTLVHRAVTVLAAKYGPLML 66

Query: 82  LKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYWRQAR 141
           L  G+ P L+VSS + A E+MKTHDL  +NRP T AA+ LLY   D+ FAPYGEYWR+ +
Sbjct: 67  LHFGKTPVLIVSSQETAKEIMKTHDLALANRPLTTAARALLYDCTDISFAPYGEYWREMK 126

Query: 142 KICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNNIVSRCIL 201
           K+  L L S K+++SF+ VR+E  + ++  I +       V+++ +    S +++ RC L
Sbjct: 127 KMAVLNLLSIKKIQSFRSVREELASDMIKEITRLSKTGAPVDVTNMLYHFSEDLLFRCTL 186

Query: 202 GEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVRGFNGELKKWFTTLDAFL 261
           G K     G+ +F   SR  + L+ AFCF DFFP + W+D + G N +LKK    LD F+
Sbjct: 187 GFK---PKGQHKFQKLSRDFLDLVGAFCFNDFFPGMAWMDALTGLNRKLKKGSRELDDFV 246

Query: 262 SKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSLENLKGIVLDMFVGGTDT 321
            +++EE  A ++ G E +E    F+ ++L   +    +   + +N+K I+LD F+GG D 
Sbjct: 247 DELIEERIAMVKDGVEPNE----FLDLLLHTHRDTTQEIKLTRDNVKAIILDTFLGGIDL 306

Query: 322 TATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHYMNCVIKESLRLHPP 381
            A+ +EW M ELMRNP  M+  QEEVRK VG K K++ +D+ +M+++   +KE+LRLHPP
Sbjct: 307 PASVMEWAMAELMRNPSKMKIAQEEVRKVVGNKNKVDEDDVYQMNFLKSAVKETLRLHPP 366

Query: 382 VPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEFIPERFMENSVDYKG 441
            PLL  RE+ + + +E Y IP  T V +N W I RDP +W++  EFIPERFM + +DYK 
Sbjct: 367 APLLFARESYTSINVENYIIPPYTSVMINIWHIQRDPKLWDKAEEFIPERFMNSGIDYKS 426

Query: 442 QDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGF---ELDVEEENGLTVR 501
            D+EFIPFGSGRR CPGMSFG+A+ E+A+ANLLYWFDWK         LD+ E+    + 
Sbjct: 427 HDYEFIPFGSGRRGCPGMSFGVAAVEFAVANLLYWFDWKFVGDTTPETLDMTEDYCFALF 486

Query: 502 KKNPLHLNPI 508
           KK PLH  PI
Sbjct: 487 KKKPLHFIPI 489

BLAST of MC03g_new0405 vs. ExPASy Swiss-Prot
Match: A0A0N9HT29 (Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71BE54 PE=1 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 2.0e-121
Identity = 229/492 (46.54%), Postives = 319/492 (64.84%), Query Frame = 0

Query: 21  SLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQL--GSLPHQSVAALSDKHGPL 80
           +L I   F+++ K  + K++    PP P KLP+IG+LH L    LPH ++  L+ KHGPL
Sbjct: 11  ALLIILLFMLVKKAIQNKNS--KLPPGPRKLPVIGSLHHLIGDGLPHHALRNLAKKHGPL 70

Query: 81  MLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYWRQ 140
           M L+LG+  TLVVSS+KMA  +M THDL F+NR    A  ILLYG K +G APYG+YWRQ
Sbjct: 71  MHLQLGENSTLVVSSSKMARAIMSTHDLMFANRTVQFATDILLYGGKGIGLAPYGDYWRQ 130

Query: 141 ARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNNIVSRC 200
            RKIC LEL SAKRV+SFQ VR+EE++   N IR        VN S++  S +N+I++R 
Sbjct: 131 IRKICVLELLSAKRVQSFQTVREEEIS---NLIRSISAESSKVNFSEMITSLTNDIIARA 190

Query: 201 ILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVRGFNGELKKWFTTLDA 260
             GEK +D   K  F       + L   F  A  FP+L  + V+ G   +L++    LD 
Sbjct: 191 AFGEKCKD---KHAFLSLIEEGIQLAGGFDIAGLFPSLKLLHVITGKKRQLERVHNELDR 250

Query: 261 FLSKVVEEHKAKMRAGG-ESDESKKDFVGIMLQLMQSDMVDYHFSLENLKGIVLDMFVGG 320
            L  +++E+K K RA     D+ +++ V +++++ ++  ++   + +NLK ++LD+FV G
Sbjct: 251 ILVDIIKENKEKRRASKLNEDKYEENLVDVLVRVQENTQLEVPLANDNLKAVILDIFVAG 310

Query: 321 TDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHYMNCVIKESLRL 380
           ++T++T +EW M+E+++NP VM+K Q EVR+      KI   +I+++ Y+  V+KE+LRL
Sbjct: 311 SETSSTTIEWAMSEMLKNPKVMKKAQAEVRRVFSGNKKINETEIQELSYLKLVLKETLRL 370

Query: 381 HPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEFIPERFMENSVD 440
           H P PLL+PRE     EI+GY IP KT V VNAW I RDP  W   + F PERF   SVD
Sbjct: 371 HAPAPLLIPRECRESCEIDGYEIPKKTMVMVNAWAIGRDPENWSNGDRFEPERFDGISVD 430

Query: 441 YKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGF---ELDVEEENGL 500
           YKG +FE+IPFG+GRRICPGM FG+A+ E  LA LLY FDW+LPNG    ELD++E  G 
Sbjct: 431 YKGTNFEYIPFGAGRRICPGMLFGMANVEVPLARLLYHFDWELPNGIKPEELDMDETFGT 490

Query: 501 TVRKKNPLHLNP 507
           TVR+KN L+L P
Sbjct: 491 TVRRKNHLYLIP 494

BLAST of MC03g_new0405 vs. NCBI nr
Match: XP_022137055.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 993 bits (2567), Expect = 0.0
Identity = 493/516 (95.54%), Postives = 501/516 (97.09%), Query Frame = 0

Query: 4   MDPISKF----QQLHFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQ 63
           MD IS F    QQLHFDLIP+SLC+ FFFLVLLKLFKPK+NT+NFPPSPPKLPIIGNLHQ
Sbjct: 1   MDLISNFLQFWQQLHFDLIPFSLCLIFFFLVLLKLFKPKNNTKNFPPSPPKLPIIGNLHQ 60

Query: 64  LGSLPHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKI 123
           LGSLPHQS+AALSDK+GPLMLLKLG A TLVVSSAKMA ELMKTHDLKFSNRPQT+ AK 
Sbjct: 61  LGSLPHQSLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFSNRPQTMPAKT 120

Query: 124 LLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCV--- 183
           LLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEV+VLVNRIRKGCV   
Sbjct: 121 LLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVNRIRKGCVDGD 180

Query: 184 GEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPAL 243
           GEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPAL
Sbjct: 181 GEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPAL 240

Query: 244 WWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDM 303
           WWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDM
Sbjct: 241 WWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDM 300

Query: 304 VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI 363
           VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI
Sbjct: 301 VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI 360

Query: 364 EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD 423
           EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD
Sbjct: 361 EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD 420

Query: 424 PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF
Sbjct: 421 PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 480

Query: 484 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHTFD 512
           DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHTFD
Sbjct: 481 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHTFD 516

BLAST of MC03g_new0405 vs. NCBI nr
Match: XP_022137054.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 450/512 (87.89%), Postives = 476/512 (92.97%), Query Frame = 0

Query: 4   MDPISKFQQL--HFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLG 63
           MDPISKF+Q+   FDLIP+SLCIFFFFLVLLKL KPK+NT+NFPPSPPKLPIIGNLHQLG
Sbjct: 1   MDPISKFEQIWQQFDLIPFSLCIFFFFLVLLKLLKPKNNTKNFPPSPPKLPIIGNLHQLG 60

Query: 64  SLPHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILL 123
           SLPHQS+AALSDKHGPLM LKLGQAPTLVVSSA+MA E+MKTHDL FS+RPQT AAKILL
Sbjct: 61  SLPHQSLAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFSDRPQTTAAKILL 120

Query: 124 YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVG---E 183
           YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIR+GCVG   E
Sbjct: 121 YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRRGCVGGDGE 180

Query: 184 GSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWW 243
           GSVNL+QLFLSTSNNIVSRC++G+KFEDENGKSRFGD SRRVMVL+TAFCFADFFPALWW
Sbjct: 181 GSVNLAQLFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRVMVLLTAFCFADFFPALWW 240

Query: 244 IDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGE-SDESKKDFVGIMLQL-MQSDM 303
           IDVVRGFNGELKK FTTLDAF SKVVEEHKAKM  GGE  DESK+DF+  MLQL +Q DM
Sbjct: 241 IDVVRGFNGELKKCFTTLDAFFSKVVEEHKAKMIGGGELCDESKRDFMDSMLQLILQDDM 300

Query: 304 VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI 363
           VDY FSL+NLK IVLDMFVGG+DTTATGLEWTMTELMRNP VM+KVQEEVRK VG K KI
Sbjct: 301 VDYRFSLDNLKAIVLDMFVGGSDTTATGLEWTMTELMRNPSVMKKVQEEVRKIVGNKEKI 360

Query: 364 EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD 423
           E+EDI+ M YMNCVIKESLRLHPPVPLLVPREAI DVEIEGYHIPSKTRVFVNAWKIHRD
Sbjct: 361 EMEDIQNMPYMNCVIKESLRLHPPVPLLVPREAIGDVEIEGYHIPSKTRVFVNAWKIHRD 420

Query: 424 PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           P IWERPNEFIPERFMENS+DYKGQDF+FIPFGSGRRICPG SFGIASFEYALANLLYWF
Sbjct: 421 PNIWERPNEFIPERFMENSIDYKGQDFDFIPFGSGRRICPGKSFGIASFEYALANLLYWF 480

Query: 484 DWKLPNGF-ELDVEEENGLTVRKKNPLHLNPI 507
           DWKLP+G  ELDVEEENGLTVRKK PLHLNPI
Sbjct: 481 DWKLPHGSKELDVEEENGLTVRKKIPLHLNPI 512

BLAST of MC03g_new0405 vs. NCBI nr
Match: XP_022137052.1 (cytochrome P450 71A1-like isoform X1 [Momordica charantia])

HSP 1 Score: 833 bits (2153), Expect = 1.35e-301
Identity = 417/514 (81.13%), Postives = 452/514 (87.94%), Query Frame = 0

Query: 4   MDPISKFQQLHFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLGSL 63
           M PISKF     DLIP SLC+ FFFLVLLKLFKPK+NT+N PPSPPKLPIIGNLHQLGSL
Sbjct: 1   MYPISKF-----DLIPLSLCLIFFFLVLLKLFKPKNNTKNLPPSPPKLPIIGNLHQLGSL 60

Query: 64  PHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYG 123
           PHQS+AALSDKHGPLMLLKLGQ PTLVVSSA+MA E+MKTHDL FS+RP + AA ILLY 
Sbjct: 61  PHQSLAALSDKHGPLMLLKLGQVPTLVVSSAEMAREVMKTHDLNFSSRPLSTAANILLYQ 120

Query: 124 FKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNL 183
             DV FAPYG+YWRQARKICALELFS KRVESFQYVRDEEVAVLV+RIR+GC GEGSVNL
Sbjct: 121 SHDVAFAPYGDYWRQARKICALELFSVKRVESFQYVRDEEVAVLVDRIRRGCDGEGSVNL 180

Query: 184 SQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVR 243
            +LFLSTSNNIVSRC++G+KFEDENGKSRFGD SRR MV+ITAFCFADF PALWWIDVVR
Sbjct: 181 CELFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRAMVIITAFCFADFLPALWWIDVVR 240

Query: 244 GFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSL 303
           GF+GELKK FTTLDAFLSKVVEEH AKMRAGGESDESKKDF+ IMLQLMQ + VDYHFSL
Sbjct: 241 GFDGELKKCFTTLDAFLSKVVEEHMAKMRAGGESDESKKDFMDIMLQLMQGEKVDYHFSL 300

Query: 304 ENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRK 363
           ++LK IV+DMFVGG+DTTATGLEWTMTELMRN   M+KVQEEVR+ VGKKA+IE+EDI K
Sbjct: 301 DHLKAIVMDMFVGGSDTTATGLEWTMTELMRNRAAMDKVQEEVRRVVGKKARIEVEDIEK 360

Query: 364 MHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERP 423
           M YM CVIKESLRLHPP+PLL+PRE + DVEIEGYHIPSK RVFVN W I RDP IWERP
Sbjct: 361 MEYMKCVIKESLRLHPPLPLLIPRETMGDVEIEGYHIPSKARVFVNVWAIQRDPKIWERP 420

Query: 424 NEFIPERFME-NSVDYKGQD------FEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           NEFIPERFME NS   KG+D      +EFI FG GRR CPGMSFG+ASFE ALANLL+WF
Sbjct: 421 NEFIPERFMEKNSGGQKGRDEYGRDEYEFISFGGGRRKCPGMSFGLASFECALANLLHWF 480

Query: 484 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHT 510
           DWKLPNG ELDVEEENGLTVRKK PLHLNPI +T
Sbjct: 481 DWKLPNGSELDVEEENGLTVRKKIPLHLNPIPYT 509

BLAST of MC03g_new0405 vs. NCBI nr
Match: XP_022137053.1 (cytochrome P450 71A1-like isoform X2 [Momordica charantia])

HSP 1 Score: 826 bits (2134), Expect = 9.77e-299
Identity = 416/514 (80.93%), Postives = 450/514 (87.55%), Query Frame = 0

Query: 4   MDPISKFQQLHFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLGSL 63
           M PISKF     DLIP SLC+ FFFLVLLKLFKPK+NT+N PPSPPKLPIIGNLHQLGSL
Sbjct: 1   MYPISKF-----DLIPLSLCLIFFFLVLLKLFKPKNNTKNLPPSPPKLPIIGNLHQLGSL 60

Query: 64  PHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYG 123
           PHQS+AALSDKHGPLMLLKLGQ PTLVVSSA+MA E+MKTHDL FS+RP + AA ILLY 
Sbjct: 61  PHQSLAALSDKHGPLMLLKLGQVPTLVVSSAEMAREVMKTHDLNFSSRPLSTAANILLYQ 120

Query: 124 FKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNL 183
             DV FAPYG+YWRQARKICALELFS KRVESFQYVRDEEVAVLV+RIR+GC GEGSVNL
Sbjct: 121 SHDVAFAPYGDYWRQARKICALELFSVKRVESFQYVRDEEVAVLVDRIRRGCDGEGSVNL 180

Query: 184 SQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVR 243
            +LFLSTSNNIVSRC++G+KFEDENGKSRFGD SRR MV+ITAFCFADF PALWWIDVVR
Sbjct: 181 CELFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRAMVIITAFCFADFLPALWWIDVVR 240

Query: 244 GFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSL 303
           GF+GELKK FTTLDAFLSKVVEEH AKMRAGGESDESKKDF+ IMLQLMQ + VDYHFSL
Sbjct: 241 GFDGELKKCFTTLDAFLSKVVEEHMAKMRAGGESDESKKDFMDIMLQLMQGEKVDYHFSL 300

Query: 304 ENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRK 363
           ++LK I  DMFVGG+DTTATGLEWTMTELMRN   M+KVQEEVR+ VGKKA+IE+EDI K
Sbjct: 301 DHLKAI--DMFVGGSDTTATGLEWTMTELMRNRAAMDKVQEEVRRVVGKKARIEVEDIEK 360

Query: 364 MHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERP 423
           M YM CVIKESLRLHPP+PLL+PRE + DVEIEGYHIPSK RVFVN W I RDP IWERP
Sbjct: 361 MEYMKCVIKESLRLHPPLPLLIPRETMGDVEIEGYHIPSKARVFVNVWAIQRDPKIWERP 420

Query: 424 NEFIPERFME-NSVDYKGQD------FEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           NEFIPERFME NS   KG+D      +EFI FG GRR CPGMSFG+ASFE ALANLL+WF
Sbjct: 421 NEFIPERFMEKNSGGQKGRDEYGRDEYEFISFGGGRRKCPGMSFGLASFECALANLLHWF 480

Query: 484 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHT 510
           DWKLPNG ELDVEEENGLTVRKK PLHLNPI +T
Sbjct: 481 DWKLPNGSELDVEEENGLTVRKKIPLHLNPIPYT 507

BLAST of MC03g_new0405 vs. NCBI nr
Match: XP_023542640.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 760 bits (1963), Expect = 2.17e-272
Identity = 382/509 (75.05%), Postives = 425/509 (83.50%), Query Frame = 0

Query: 2   EKMDPISKFQQLHFDLIP--WSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQ 61
           EKMD  +K QQ    L P  +SLC+FFFF+VLLKLFK   N  NFPPSPP+LPIIGNLHQ
Sbjct: 21  EKMDFPTKIQQQFQALNPISFSLCLFFFFIVLLKLFK--RNKPNFPPSPPRLPIIGNLHQ 80

Query: 62  LGSLPHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKI 121
           LG+LPHQS+AALS+K+GPLMLLKLGQ  TLVVSSAKMA E+MKT+D+KFSNRPQT AA  
Sbjct: 81  LGALPHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTYDIKFSNRPQTTAAYE 140

Query: 122 LLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEG 181
           LLY  +D+GF  YGEYWRQARK+CALELFS KRV+ FQYVRDEEV  L++RI K C+G  
Sbjct: 141 LLYQCQDIGFTQYGEYWRQARKVCALELFSVKRVDFFQYVRDEEVGELIDRIGKACMGGE 200

Query: 182 SVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWI 241
            VNLSQLF  TSNNIVSRC+LGEKFEDENG SRFGDT+R+VMVLITAFC AD FP LWWI
Sbjct: 201 GVNLSQLFPQTSNNIVSRCVLGEKFEDENGNSRFGDTARKVMVLITAFCVADLFPCLWWI 260

Query: 242 DVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDY 301
           D +RGFN ELK  F  LD F SKVVEEHK K+R G  +DESKKDFV IMLQL +  MVDY
Sbjct: 261 DTIRGFNKELKDCFKRLDTFFSKVVEEHKEKIRDGVGTDESKKDFVDIMLQLQRDGMVDY 320

Query: 302 HFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIE 361
           HFS +NLK IVLDMFVGG+DTTAT LEWTMTELMRNP  M+K QEEVR  +GKK KIE E
Sbjct: 321 HFSPDNLKAIVLDMFVGGSDTTATVLEWTMTELMRNPAAMKKAQEEVRTIIGKKPKIEAE 380

Query: 362 DIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTI 421
           DI+KM Y+ CVIKESLRLHPP+PLLVPRE   DVEI+GYHIPSKTRVFVNAWKI RDP  
Sbjct: 381 DIQKMEYIQCVIKESLRLHPPIPLLVPRETAEDVEIDGYHIPSKTRVFVNAWKIQRDPEN 440

Query: 422 WERPNEFIPERFME-NSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDW 481
           W  PNEFIPERFM+ +SVDYKGQD+EFIPFGSGRR CPG+SFG ASFE+ALANLL+WFDW
Sbjct: 441 WHNPNEFIPERFMDKSSVDYKGQDYEFIPFGSGRRKCPGLSFGAASFEHALANLLHWFDW 500

Query: 482 KLPNGFELDVEEENGLTVRKKNPLHLNPI 507
           KLP+  +L VEEENGLTVRKK PL LNP+
Sbjct: 501 KLPSDCDLSVEEENGLTVRKKTPLLLNPM 527

BLAST of MC03g_new0405 vs. ExPASy TrEMBL
Match: A0A6J1C5L5 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV=1)

HSP 1 Score: 993 bits (2567), Expect = 0.0
Identity = 493/516 (95.54%), Postives = 501/516 (97.09%), Query Frame = 0

Query: 4   MDPISKF----QQLHFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQ 63
           MD IS F    QQLHFDLIP+SLC+ FFFLVLLKLFKPK+NT+NFPPSPPKLPIIGNLHQ
Sbjct: 1   MDLISNFLQFWQQLHFDLIPFSLCLIFFFLVLLKLFKPKNNTKNFPPSPPKLPIIGNLHQ 60

Query: 64  LGSLPHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKI 123
           LGSLPHQS+AALSDK+GPLMLLKLG A TLVVSSAKMA ELMKTHDLKFSNRPQT+ AK 
Sbjct: 61  LGSLPHQSLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFSNRPQTMPAKT 120

Query: 124 LLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCV--- 183
           LLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEV+VLVNRIRKGCV   
Sbjct: 121 LLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVNRIRKGCVDGD 180

Query: 184 GEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPAL 243
           GEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPAL
Sbjct: 181 GEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPAL 240

Query: 244 WWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDM 303
           WWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDM
Sbjct: 241 WWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDM 300

Query: 304 VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI 363
           VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI
Sbjct: 301 VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI 360

Query: 364 EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD 423
           EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD
Sbjct: 361 EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD 420

Query: 424 PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF
Sbjct: 421 PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 480

Query: 484 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHTFD 512
           DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHTFD
Sbjct: 481 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHTFD 516

BLAST of MC03g_new0405 vs. ExPASy TrEMBL
Match: A0A6J1C631 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV=1)

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 450/512 (87.89%), Postives = 476/512 (92.97%), Query Frame = 0

Query: 4   MDPISKFQQL--HFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLG 63
           MDPISKF+Q+   FDLIP+SLCIFFFFLVLLKL KPK+NT+NFPPSPPKLPIIGNLHQLG
Sbjct: 1   MDPISKFEQIWQQFDLIPFSLCIFFFFLVLLKLLKPKNNTKNFPPSPPKLPIIGNLHQLG 60

Query: 64  SLPHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILL 123
           SLPHQS+AALSDKHGPLM LKLGQAPTLVVSSA+MA E+MKTHDL FS+RPQT AAKILL
Sbjct: 61  SLPHQSLAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFSDRPQTTAAKILL 120

Query: 124 YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVG---E 183
           YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIR+GCVG   E
Sbjct: 121 YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRRGCVGGDGE 180

Query: 184 GSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWW 243
           GSVNL+QLFLSTSNNIVSRC++G+KFEDENGKSRFGD SRRVMVL+TAFCFADFFPALWW
Sbjct: 181 GSVNLAQLFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRVMVLLTAFCFADFFPALWW 240

Query: 244 IDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGE-SDESKKDFVGIMLQL-MQSDM 303
           IDVVRGFNGELKK FTTLDAF SKVVEEHKAKM  GGE  DESK+DF+  MLQL +Q DM
Sbjct: 241 IDVVRGFNGELKKCFTTLDAFFSKVVEEHKAKMIGGGELCDESKRDFMDSMLQLILQDDM 300

Query: 304 VDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKI 363
           VDY FSL+NLK IVLDMFVGG+DTTATGLEWTMTELMRNP VM+KVQEEVRK VG K KI
Sbjct: 301 VDYRFSLDNLKAIVLDMFVGGSDTTATGLEWTMTELMRNPSVMKKVQEEVRKIVGNKEKI 360

Query: 364 EIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRD 423
           E+EDI+ M YMNCVIKESLRLHPPVPLLVPREAI DVEIEGYHIPSKTRVFVNAWKIHRD
Sbjct: 361 EMEDIQNMPYMNCVIKESLRLHPPVPLLVPREAIGDVEIEGYHIPSKTRVFVNAWKIHRD 420

Query: 424 PTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           P IWERPNEFIPERFMENS+DYKGQDF+FIPFGSGRRICPG SFGIASFEYALANLLYWF
Sbjct: 421 PNIWERPNEFIPERFMENSIDYKGQDFDFIPFGSGRRICPGKSFGIASFEYALANLLYWF 480

Query: 484 DWKLPNGF-ELDVEEENGLTVRKKNPLHLNPI 507
           DWKLP+G  ELDVEEENGLTVRKK PLHLNPI
Sbjct: 481 DWKLPHGSKELDVEEENGLTVRKKIPLHLNPI 512

BLAST of MC03g_new0405 vs. ExPASy TrEMBL
Match: A0A6J1C752 (cytochrome P450 71A1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008613 PE=3 SV=1)

HSP 1 Score: 833 bits (2153), Expect = 6.53e-302
Identity = 417/514 (81.13%), Postives = 452/514 (87.94%), Query Frame = 0

Query: 4   MDPISKFQQLHFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLGSL 63
           M PISKF     DLIP SLC+ FFFLVLLKLFKPK+NT+N PPSPPKLPIIGNLHQLGSL
Sbjct: 1   MYPISKF-----DLIPLSLCLIFFFLVLLKLFKPKNNTKNLPPSPPKLPIIGNLHQLGSL 60

Query: 64  PHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYG 123
           PHQS+AALSDKHGPLMLLKLGQ PTLVVSSA+MA E+MKTHDL FS+RP + AA ILLY 
Sbjct: 61  PHQSLAALSDKHGPLMLLKLGQVPTLVVSSAEMAREVMKTHDLNFSSRPLSTAANILLYQ 120

Query: 124 FKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNL 183
             DV FAPYG+YWRQARKICALELFS KRVESFQYVRDEEVAVLV+RIR+GC GEGSVNL
Sbjct: 121 SHDVAFAPYGDYWRQARKICALELFSVKRVESFQYVRDEEVAVLVDRIRRGCDGEGSVNL 180

Query: 184 SQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVR 243
            +LFLSTSNNIVSRC++G+KFEDENGKSRFGD SRR MV+ITAFCFADF PALWWIDVVR
Sbjct: 181 CELFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRAMVIITAFCFADFLPALWWIDVVR 240

Query: 244 GFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSL 303
           GF+GELKK FTTLDAFLSKVVEEH AKMRAGGESDESKKDF+ IMLQLMQ + VDYHFSL
Sbjct: 241 GFDGELKKCFTTLDAFLSKVVEEHMAKMRAGGESDESKKDFMDIMLQLMQGEKVDYHFSL 300

Query: 304 ENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRK 363
           ++LK IV+DMFVGG+DTTATGLEWTMTELMRN   M+KVQEEVR+ VGKKA+IE+EDI K
Sbjct: 301 DHLKAIVMDMFVGGSDTTATGLEWTMTELMRNRAAMDKVQEEVRRVVGKKARIEVEDIEK 360

Query: 364 MHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERP 423
           M YM CVIKESLRLHPP+PLL+PRE + DVEIEGYHIPSK RVFVN W I RDP IWERP
Sbjct: 361 MEYMKCVIKESLRLHPPLPLLIPRETMGDVEIEGYHIPSKARVFVNVWAIQRDPKIWERP 420

Query: 424 NEFIPERFME-NSVDYKGQD------FEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           NEFIPERFME NS   KG+D      +EFI FG GRR CPGMSFG+ASFE ALANLL+WF
Sbjct: 421 NEFIPERFMEKNSGGQKGRDEYGRDEYEFISFGGGRRKCPGMSFGLASFECALANLLHWF 480

Query: 484 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHT 510
           DWKLPNG ELDVEEENGLTVRKK PLHLNPI +T
Sbjct: 481 DWKLPNGSELDVEEENGLTVRKKIPLHLNPIPYT 509

BLAST of MC03g_new0405 vs. ExPASy TrEMBL
Match: A0A6J1C977 (cytochrome P450 71A1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008613 PE=3 SV=1)

HSP 1 Score: 826 bits (2134), Expect = 4.73e-299
Identity = 416/514 (80.93%), Postives = 450/514 (87.55%), Query Frame = 0

Query: 4   MDPISKFQQLHFDLIPWSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLGSL 63
           M PISKF     DLIP SLC+ FFFLVLLKLFKPK+NT+N PPSPPKLPIIGNLHQLGSL
Sbjct: 1   MYPISKF-----DLIPLSLCLIFFFLVLLKLFKPKNNTKNLPPSPPKLPIIGNLHQLGSL 60

Query: 64  PHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYG 123
           PHQS+AALSDKHGPLMLLKLGQ PTLVVSSA+MA E+MKTHDL FS+RP + AA ILLY 
Sbjct: 61  PHQSLAALSDKHGPLMLLKLGQVPTLVVSSAEMAREVMKTHDLNFSSRPLSTAANILLYQ 120

Query: 124 FKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNL 183
             DV FAPYG+YWRQARKICALELFS KRVESFQYVRDEEVAVLV+RIR+GC GEGSVNL
Sbjct: 121 SHDVAFAPYGDYWRQARKICALELFSVKRVESFQYVRDEEVAVLVDRIRRGCDGEGSVNL 180

Query: 184 SQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVR 243
            +LFLSTSNNIVSRC++G+KFEDENGKSRFGD SRR MV+ITAFCFADF PALWWIDVVR
Sbjct: 181 CELFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRAMVIITAFCFADFLPALWWIDVVR 240

Query: 244 GFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSL 303
           GF+GELKK FTTLDAFLSKVVEEH AKMRAGGESDESKKDF+ IMLQLMQ + VDYHFSL
Sbjct: 241 GFDGELKKCFTTLDAFLSKVVEEHMAKMRAGGESDESKKDFMDIMLQLMQGEKVDYHFSL 300

Query: 304 ENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRK 363
           ++LK I  DMFVGG+DTTATGLEWTMTELMRN   M+KVQEEVR+ VGKKA+IE+EDI K
Sbjct: 301 DHLKAI--DMFVGGSDTTATGLEWTMTELMRNRAAMDKVQEEVRRVVGKKARIEVEDIEK 360

Query: 364 MHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERP 423
           M YM CVIKESLRLHPP+PLL+PRE + DVEIEGYHIPSK RVFVN W I RDP IWERP
Sbjct: 361 MEYMKCVIKESLRLHPPLPLLIPRETMGDVEIEGYHIPSKARVFVNVWAIQRDPKIWERP 420

Query: 424 NEFIPERFME-NSVDYKGQD------FEFIPFGSGRRICPGMSFGIASFEYALANLLYWF 483
           NEFIPERFME NS   KG+D      +EFI FG GRR CPGMSFG+ASFE ALANLL+WF
Sbjct: 421 NEFIPERFMEKNSGGQKGRDEYGRDEYEFISFGGGRRKCPGMSFGLASFECALANLLHWF 480

Query: 484 DWKLPNGFELDVEEENGLTVRKKNPLHLNPIFHT 510
           DWKLPNG ELDVEEENGLTVRKK PLHLNPI +T
Sbjct: 481 DWKLPNGSELDVEEENGLTVRKKIPLHLNPIPYT 507

BLAST of MC03g_new0405 vs. ExPASy TrEMBL
Match: A0A6J1GRT5 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=1)

HSP 1 Score: 751 bits (1940), Expect = 1.65e-269
Identity = 380/507 (74.95%), Postives = 424/507 (83.63%), Query Frame = 0

Query: 4   MDPISKFQQLHFDLIP--WSLCIFFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLG 63
           MD   K QQ    L P   SLC+FFFF++LLKLF+ K    NFPPSPP+LPIIGNLHQLG
Sbjct: 1   MDLTRKIQQQFQALNPISLSLCLFFFFVLLLKLFERKKP--NFPPSPPRLPIIGNLHQLG 60

Query: 64  SLPHQSVAALSDKHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILL 123
           +LPHQS+AALS+K+GPLMLLKLGQ   LVVSS KMA E+MKTHD+KFSNRPQT AA  LL
Sbjct: 61  ALPHQSMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAHELL 120

Query: 124 YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSV 183
           Y  +D+GFA YGEYWRQARK+CALELFS KRV+SFQYVRDEEV  L++RIRK CVG  +V
Sbjct: 121 YQCQDIGFAQYGEYWRQARKVCALELFSVKRVDSFQYVRDEEVGGLMDRIRKACVGGEAV 180

Query: 184 NLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDV 243
           NLSQLFL TSNNIVSRC+LGEKFEDENG SRFG T RR MVLI AFC AD FP+LWWID+
Sbjct: 181 NLSQLFLQTSNNIVSRCVLGEKFEDENGNSRFGVTVRRAMVLIVAFCVADLFPSLWWIDI 240

Query: 244 VRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHF 303
           +RGFN ELK    TLD F+SKV+EEHK K+R G  +DESKKDFV IMLQL Q DMVDYHF
Sbjct: 241 IRGFNKELKDCSKTLDTFVSKVIEEHKEKIRDGVGTDESKKDFVDIMLQLQQDDMVDYHF 300

Query: 304 SLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDI 363
           S ++LK ++LDMFVGG+DTTAT LEWTMTELMRNP  M+KVQEEVR   GKK+KIE+EDI
Sbjct: 301 SPDSLKAMLLDMFVGGSDTTATTLEWTMTELMRNPSTMKKVQEEVRTITGKKSKIEVEDI 360

Query: 364 RKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWE 423
           +KM YM+CVI ESLRLHPPVPLLVPRE  +DVEI+GYHIPSKTRVFVNAWKI RD   W+
Sbjct: 361 QKMEYMHCVINESLRLHPPVPLLVPRETAADVEIDGYHIPSKTRVFVNAWKIQRDLEYWK 420

Query: 424 RPNEFIPERFMEN-SVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKL 483
            P EFIPERFM+N SVDYKGQ +EFIPFGSGRR CPGMSFG+ASFE ALANLL+WFDWKL
Sbjct: 421 NPTEFIPERFMDNRSVDYKGQCYEFIPFGSGRRKCPGMSFGVASFEQALANLLHWFDWKL 480

Query: 484 PNGFELDVEEENGLTVRKKNPLHLNPI 507
           P+G EL VEEE GLTV KK PL LNPI
Sbjct: 481 PSGCELSVEEEGGLTVCKKIPLLLNPI 505

BLAST of MC03g_new0405 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 435.3 bits (1118), Expect = 7.0e-122
Identity = 224/504 (44.44%), Postives = 325/504 (64.48%), Query Frame = 0

Query: 22  LCIFFFFLVLLKLF--------KPKSNTRNFPPSPPKLPIIGNLHQLGSLPHQSVAALSD 81
           + IF  FL+LL L         K +S+    PP PPKLPIIGNLH L  LPH+ +  L  
Sbjct: 1   MSIFLCFLLLLLLLLVTIIFTRKSQSSKLKLPPGPPKLPIIGNLHYLNGLPHKCLLNLWK 60

Query: 82  KHGPLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYG 141
            HGP+M L+LG  P +V+SS + A E++KTHDL   +RP+T+A+K + Y FKD+GFAPYG
Sbjct: 61  IHGPVMQLQLGYVPLVVISSNQAAEEVLKTHDLDCCSRPETIASKTISYNFKDIGFAPYG 120

Query: 142 EYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNN 201
           E WR  RK+  +ELFS K+  SF+Y+R+EE  +LV ++ +    +  VNL +   + S +
Sbjct: 121 EEWRALRKLAVIELFSLKKFNSFRYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSAS 180

Query: 202 IVSRCILGE-----KFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVRGFNGE 261
           IV R   G+     +F DE+        S ++      F F++FFP  W +D + G +  
Sbjct: 181 IVCRLAFGQNLHESEFIDEDSMEDLASRSEKIQ---AKFAFSNFFPGGWILDKITGQSKS 240

Query: 262 LKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSL--ENL 321
           L + F  LD F ++V+++H   ++ G +  E+  D V +M+ +M     D  F L  +++
Sbjct: 241 LNEIFADLDGFFNQVLDDH---LKPGRKVLET-PDVVDVMIDMMNKQSQDGSFKLTTDHI 300

Query: 322 KGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVG-KKAKIEIEDIRKMH 381
           KGI+ D+F+ G +T+AT + W MTEL+RNP VM+KVQ+EVR  +G K+ +I  +D+ +++
Sbjct: 301 KGIISDIFLAGVNTSATTILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLN 360

Query: 382 YMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNE 441
           Y   VIKE+ RLHP  PLL+PREA++ ++I+GY IP KT++ VN + I RDP +WE P E
Sbjct: 361 YFKLVIKETFRLHPAAPLLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEE 420

Query: 442 FIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNG-- 501
           F PERF+++SVDY+G +FE +PFGSGRRICPGM+ GIA+ E  L NLLY+FDW LP G  
Sbjct: 421 FKPERFVDSSVDYRGLNFELLPFGSGRRICPGMTMGIATVELGLLNLLYFFDWGLPEGRT 480

Query: 502 -FELDVEEENGLTVRKKNPLHLNP 507
             ++D+EEE  + + KK  L L P
Sbjct: 481 VKDIDLEEEGAIIIGKKVSLELVP 497

BLAST of MC03g_new0405 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 429.9 bits (1104), Expect = 2.9e-120
Identity = 222/505 (43.96%), Postives = 329/505 (65.15%), Query Frame = 0

Query: 22  LCIFFFFLVLLK----LFKPKSNTRNFPPSPPKLPIIGNLHQLGSLPHQSVAALSDKHGP 81
           LC F   L+ +     L + K++  N PPSP  LPIIGNLH L  LPH+    LS K+GP
Sbjct: 5   LCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGP 64

Query: 82  LMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYWR 141
           L+ L+LG  P +V+SS++ A  ++KT+DL+  +RP+TV +  L YGFKD+ FAPYGEYWR
Sbjct: 65  LVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWR 124

Query: 142 QARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNNIVSR 201
           + RK+  +ELFS+K+V+SF+Y+R+EEV  +V ++ +  + +  V+LS+ F S + +I+ R
Sbjct: 125 EVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICR 184

Query: 202 CILGEKFEDENGKSRFGDTSRRVMVLIT-------AFCFADFFPALW--WIDVVRGFNGE 261
             LG+ F +    S F     R+  L+T        F F+DFFP     ++D +   + +
Sbjct: 185 VALGQNFNE----SGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKK 244

Query: 262 LKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLM--QSDMVDYHFSLENL 321
           + K F  LDAF   V+++H   ++  G  ++   D V ++L ++  Q D   +  +++NL
Sbjct: 245 INKVFKELDAFYQHVIDDH---LKPEGRKNQ---DIVTLILDMIDKQEDSDSFKLNMDNL 304

Query: 322 KGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVG-KKAKIEIEDIRKMH 381
           K IV+D+F+ G DT+A  + W MTEL+RNP VM+K QE +R T+G KK +I  ED+ K+ 
Sbjct: 305 KAIVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVE 364

Query: 382 YMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNE 441
           Y+N ++KE+ RLHP +P +VPRE +S ++I+GY IP KT++ +N W I RDP  W  P E
Sbjct: 365 YLNHILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEE 424

Query: 442 FIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGF- 501
           F PERF  +SVD++GQ F+ +PFGSGRRICPGM   IAS E AL NLLY+FDW +P+G  
Sbjct: 425 FNPERFANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTK 484

Query: 502 --ELDVEEENGLTVRKKNPLHLNPI 508
             ++D+EE   +++ KK PL L P+
Sbjct: 485 GEDIDMEEAGNISIVKKIPLQLVPV 499

BLAST of MC03g_new0405 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 422.5 bits (1085), Expect = 4.7e-118
Identity = 217/497 (43.66%), Postives = 310/497 (62.37%), Query Frame = 0

Query: 20  WSLCIFFFFLVLLKLFKPKSNTRNF---PPSPPKLPIIGNLHQLGSLPHQSVAALSDKHG 79
           W L + F   +L+ +F  K N RN+   PPSPP  PIIGNLHQLG LPHQS+  LS K+G
Sbjct: 5   WLLSLIFVICILVAVFNHK-NRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYG 64

Query: 80  PLMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYW 139
           P+MLLKLG+ PT++VSS++ A + +K HDL   +RP    A+ L Y + D+ F+PY +YW
Sbjct: 65  PVMLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYW 124

Query: 140 RQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNNIVS 199
           ++ RK+   ELFS+K+V S Q ++DEEV  L++ I +    +  +NL++  L+ + ++V 
Sbjct: 125 KEVRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVC 184

Query: 200 RCILGEKFEDE-NGKSRFGDTSRRVMVLITAFCFADFFPALW-WIDVVRGFNGELKKWFT 259
           R      FE       RF +  R  + ++ +F  +DF P +   ID++ G  G  ++   
Sbjct: 185 RTAFSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMR 244

Query: 260 TLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMV--DYHFSLENLKGIVLD 319
            LDAF  ++ + HK K   G E      DFV ++L+L + + V  +   +  ++K I++D
Sbjct: 245 DLDAFYEQMFDLHKQKKEEGSE------DFVDLLLRLEKEEAVLGNDKLTRNHIKAILMD 304

Query: 320 MFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHYMNCVIK 379
           + + G DT+A  + W M EL +NP VM+KVQ E+R  +  K +I  +D  K+ Y+  VIK
Sbjct: 305 VLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIK 364

Query: 380 ESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEFIPERFM 439
           E+ RLHP  PLL+PREA+S+ EI GY IP KTR+ VN W I RDP  W+ P  F+PERF 
Sbjct: 365 ETWRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFT 424

Query: 440 ENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGF---ELDVE 499
           +N++D KGQ FE +PFG GRR+CP +  G    E+ LANLLY FDWKLP G    ++D+E
Sbjct: 425 DNNIDAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDME 484

Query: 500 EENGLTVRKKNPLHLNP 507
           E  GLTV KKN L L P
Sbjct: 485 EAPGLTVNKKNELILVP 494

BLAST of MC03g_new0405 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 421.8 bits (1083), Expect = 8.0e-118
Identity = 213/489 (43.56%), Postives = 312/489 (63.80%), Query Frame = 0

Query: 21  SLCI--FFFFLVLLKLFKPKSNTRNFPPSPPKLPIIGNLHQLGSLPHQSVAALSDKHGPL 80
           SLC+      L+L K  K  +   N PPSP ++P+IGNLHQL   PH+S+ +LS ++GPL
Sbjct: 8   SLCLTTLLTLLLLKKFLKRTAKKVNLPPSPWRIPVIGNLHQLSLHPHRSLHSLSLRYGPL 67

Query: 81  MLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYWRQ 140
           MLL  G+ P LVVSS++ A E++KTHDLKF+NRP++ A   L+ G +DV F PYGEYWRQ
Sbjct: 68  MLLHFGRVPILVVSSSEAAHEILKTHDLKFANRPKSKAVHGLMNGGRDVVFGPYGEYWRQ 127

Query: 141 ARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNNIVSRC 200
            + +C L L + K V SF+ VR+EEV  ++ ++ K      + NLS+LF++ ++++ SR 
Sbjct: 128 MKSVCILNLLTNKMVASFEKVREEEVNAMMEKLEKASCSSSAENLSELFVTLTSDVTSRV 187

Query: 201 ILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWIDVVRGFNGELKKWFTTLDA 260
            LG+K+ ++          R++M L+  F   D+ PAL WID + GFN ++ +       
Sbjct: 188 SLGKKYWEDETAGGLKKRVRQIMELLREFPIGDYVPALAWIDRINGFNSKIVEVSRAYSD 247

Query: 261 FLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVDYHFSLENLKGIVLDMFVGGT 320
            + KVV+EH        E+ E K DFV I+L + +     +     ++K ++LDMF+GG 
Sbjct: 248 LMEKVVQEHL-------EAGEHKADFVNILLSIEKEKNNGFKVQRNDIKFMILDMFIGGI 307

Query: 321 DTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAK-IEIEDIRKMHYMNCVIKESLRL 380
            T++T LEW MTEL+RNP  M+K+Q E+R T+      I+ +++  M Y+  VIKE  R+
Sbjct: 308 STSSTLLEWIMTELIRNPECMKKLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRV 367

Query: 381 HPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIW-ERPNEFIPERFMENSV 440
           HPP+PL++PR    DV+++GY I + T V +NAW IHRDP IW     EF PER +++++
Sbjct: 368 HPPLPLILPRLLTEDVKVKGYDIAAGTEVLINAWSIHRDPAIWGPDAEEFKPERHLDSTL 427

Query: 441 DYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKL---PNGFELDVEEENG 500
           DY GQD ++IPFGSGRRICPG++  +   E  LANL+  FDW +   PNG + D+ E+ G
Sbjct: 428 DYHGQDLKYIPFGSGRRICPGINLAMGLVEVTLANLVGRFDWSVDPGPNGDQPDLAEDFG 487

Query: 501 LTVRKKNPL 503
           L V +KNPL
Sbjct: 488 LDVCRKNPL 489

BLAST of MC03g_new0405 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 420.6 bits (1080), Expect = 1.8e-117
Identity = 219/496 (44.15%), Postives = 306/496 (61.69%), Query Frame = 0

Query: 20  WSLCIFFFFLVLLKLFKPKSNT--RNFPPSPPKLPIIGNLHQLGSLPHQSVAALSDKHGP 79
           W L + F   +LL +F  K +   R F P PP  PIIGNLHQ+G LPHQ++  LS K+GP
Sbjct: 5   WLLPLIFLVCILLAVFNHKKHPKYRQF-PCPPGFPIIGNLHQIGELPHQTLWKLSKKYGP 64

Query: 80  LMLLKLGQAPTLVVSSAKMAIELMKTHDLKFSNRPQTVAAKILLYGFKDVGFAPYGEYWR 139
           +M L LG+ PT+VVSS+  A ++++ HDL    RP     + L Y + D+ F+PY +YW+
Sbjct: 65  VMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWK 124

Query: 140 QARKICALELFSAKRVESFQYVRDEEVAVLVNRIRKGCVGEGSVNLSQLFLSTSNNIVSR 199
           + RK+C  ELFS K+V S Q ++DEEV  +++ I +    +  VNL+   L  + ++V R
Sbjct: 125 EVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCR 184

Query: 200 CILGEKFEDE-NGKSRFGDTSRRVMVLITAFCFADFFPALWW-IDVVRGFNGELKKWFTT 259
              G  FE       RF    R  + ++ +F  ADF P + W IDV+ G  G  ++    
Sbjct: 185 TAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRD 244

Query: 260 LDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMV--DYHFSLENLKGIVLDM 319
           L+AF  ++ + HK   + G E      DFV ++L+L + + V  +   +  ++K I+LD+
Sbjct: 245 LNAFFEQMFDLHKEGKKEGNE------DFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDV 304

Query: 320 FVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHYMNCVIKE 379
            + G DT+A  + W MTEL RNP VM+KVQ E+R  +G ++ I  ED+ ++ Y+  VIKE
Sbjct: 305 LLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKE 364

Query: 380 SLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEFIPERFME 439
           + RLHP  PLL+PREA+S+ +I GY IP KTR+ VN W I RDP  W+ P  F+PERFM+
Sbjct: 365 TWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMD 424

Query: 440 NSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGFE---LDVEE 499
           N++D KGQ FE +PFG GRRICP +  G    E+ LANLLY FDWKLP G E   +DVEE
Sbjct: 425 NNIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEE 484

Query: 500 ENGLTVRKKNPLHLNP 507
             GLTV KKN L L P
Sbjct: 485 APGLTVNKKNELLLVP 493