MC03g_new0305 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0305 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 3.6e-158
Identity = 276/533 (51.78%), Postives = 372/533 (69.79%), Query Frame = 0

Query: 2   KSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPS--YSSVLN 61
           K   TL   T +++ V  +S+   +DF+ CL  +S     I+   ++P   +  +   L 
Sbjct: 7   KRNATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELE 66

Query: 62  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-- 121
            +  NLR+ T   PKP  I  PL  + VQA++VCAK     +R RSGGHDYEGLS+V+  
Sbjct: 67  STAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAED 126

Query: 122 DVSFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGG 181
           +  F++VDL  LR +++D++   AW  +GATIGE+YYRI EKS+T GFPAG+C ++G+GG
Sbjct: 127 ETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGG 186

Query: 182 HFSGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLA 241
           H  GG YG M+RKFGL ADNV+DA  +D NG++ DR +MGEDVFWA+RGGGG SFG++LA
Sbjct: 187 HLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILA 246

Query: 242 WKIKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGE 301
           WKIKL+PVP  VTVFTVT+ LEQ+ T ++++W+ +ADK DD LFIR+I+   + T + G 
Sbjct: 247 WKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG- 306

Query: 302 KRTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEV 361
            RTI  ++ + FLG   +LL +M K+FP LGL K+DC +MSWI+S++Y AGFP     E 
Sbjct: 307 NRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEA 366

Query: 362 LLDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISE 421
           LL  + L  K  FKAKSD+V EPIP  GLEG+W+ F EED+     I +PYGG M+ ISE
Sbjct: 367 LLAGKSLF-KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISE 426

Query: 422 SEIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRD 481
           SEIPFPHR G L+KIQ L  W ++G+   +RH+ WIR++YSY+  +VSKNPR AY+NYRD
Sbjct: 427 SEIPFPHRNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRD 486

Query: 482 LDIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPS 531
           LD+GTN   G T   +A  WG KY+KGNF+RLV +K + DP NFFR+EQS+P+
Sbjct: 487 LDLGTN--EGET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of MC03g_new0305 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 5.3e-157
Identity = 273/528 (51.70%), Postives = 374/528 (70.83%), Query Frame = 0

Query: 7   LLAYTFVVLFVS-----AASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNFS 66
           L+ +T   L +S     +A+  N   FL CLSL   + + +S +I+TP + S+SSVL  S
Sbjct: 11  LIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASS 70

Query: 67  THNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVSF 126
             N RF+  + PKP +I+TP+Q S VQ+++ CA+  G  IRTRSGGHDYEGLSYV+   F
Sbjct: 71  IQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPF 130

Query: 127 IVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSG 186
           +++DL NLRSI +DV+ +  WVQ+GATIGELYY I +K++TL FPAGVCPTVGVGGHFSG
Sbjct: 131 VILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSG 190

Query: 187 GGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKIK 246
           GGYG +LRK GLAAD+V+DA  +D  G++ +R+ MGED FWA+RGGGG+SF +VL+WKI 
Sbjct: 191 GGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIG 250

Query: 247 LLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRTI 306
           L+ VP  VTVF VT+  EQ+A  ++HRWQ+VADK  D LFIR++L+      +Y  K  +
Sbjct: 251 LINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQ------RY--KNMV 310

Query: 307 EAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLDR 366
            A+F  L+LG V+ LL +++K FP LGL ++DC +MSWIES+++FA     E + VL  R
Sbjct: 311 RASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGE-EPINVLTKR 370

Query: 367 RPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEIP 426
                   FKAKSD+V EP+PK  +  +W+   E +AE A++I +P+GGKM+EI++ E P
Sbjct: 371 TR--ASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETP 430

Query: 427 FPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIG 486
           FPHR GN+Y+IQ+L YW     DV ++++ W+ ++Y  ++ FV+K+PR AYIN RDLD+G
Sbjct: 431 FPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLG 490

Query: 487 TNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
                  + Y E   WGVKYFK NF+RLV VKT +DPS+FF +EQSIP
Sbjct: 491 MYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MC03g_new0305 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 2.0e-156
Identity = 272/532 (51.13%), Postives = 372/532 (69.92%), Query Frame = 0

Query: 5   ITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTP--TNPSYSSVLNFST 64
           + LL+ +++ L  S       +DF+ CL  +S     + +  +T      S+   L  S 
Sbjct: 16  LLLLSLSYIPLSFSTI----QQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 75

Query: 65  HNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV--S 124
            NLR+     PKP+ I  PL  + VQA+++CAK     +R RSGGHDYEGLSYVS++  +
Sbjct: 76  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 135

Query: 125 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 184
           F++VDL  LR I++D+E   AWV +GA+IGE+YYRI EKSK  GFPAG+C ++G+GGH  
Sbjct: 136 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 195

Query: 185 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 244
           GG YG M+RKFGL ADNV+DA  +D +GK+ +R +MGEDVFWA+RGGGG SFG++LAWKI
Sbjct: 196 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 255

Query: 245 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 304
           KL+PVP +VTVFTVTR LEQ+ T L+++WQ VADK D+ LFIR+I++  + T +  ++RT
Sbjct: 256 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPK-SKERT 315

Query: 305 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 364
           I  ++   FLG   +LL +M ++FP LGL K+DC++ SWI+S++Y AGFP     E LLD
Sbjct: 316 ISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD 375

Query: 365 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 424
            + L  K +FKAKSDYV EPIP  GLEG+W+   EED+     I +PYGG M +I E+E 
Sbjct: 376 GKSLF-KNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETET 435

Query: 425 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 484
           PFPHR+G L+KIQ L  W ++G+    +H+ W+R++YSY+  +VSK+PR+AY+NYRDLD+
Sbjct: 436 PFPHRSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDL 495

Query: 485 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLL 533
           G N K       +A  WG +YFKGNF+RLV +K K DP NFFR+EQSIP+ L
Sbjct: 496 GMNGKG-----SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of MC03g_new0305 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 2.2e-155
Identity = 269/516 (52.13%), Postives = 366/516 (70.93%), Query Frame = 0

Query: 19  AASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNFSTHNLRFTTSETPKPQVI 78
           +A   NHE+FL CLS H  N    S II+T  +PSY S+LN S  N RF   ETPKP  I
Sbjct: 28  SAQRTNHENFLKCLS-HRINEDD-SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSI 87

Query: 79  VTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-DVSFIVVDLINLRSINIDVE 138
           +TP+Q + VQ++I CA+ +G  IRTRSGGHDYEGLSY++    F+V+DL NLRSI +DV+
Sbjct: 88  ITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVD 147

Query: 139 KKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADN 198
            +  WVQSGATIGELYY I + SK+L FPAG+ PTVG+GG F GGGYG ++RK+GL+ADN
Sbjct: 148 NRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADN 207

Query: 199 VVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKIKLLPVPPVVTVFTVTRN 258
           V+DA+ +D NG   DR+ MGED FWA+RGGGG+SF +VL+WKI+LL VP VVTVF V + 
Sbjct: 208 VIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKT 267

Query: 259 LEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRTIEAAFNSLFLGKVEKLL 318
            E+ A  ++++WQY+ADK  + LFIR +L+         ++  + A+F  L+LG V  LL
Sbjct: 268 SEKEAVSIINKWQYIADKVPNDLFIRAMLQ---------KETEVYASFPGLYLGPVSDLL 327

Query: 319 SIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLDRRPLVPKRFFKAKSDYV 378
           ++M   FP LGL   +C +MSWIES+L+F    +GE +E+L  R+     R FK K D++
Sbjct: 328 ALMKDKFPELGLEIGNCREMSWIESVLWFI---KGESMEILAKRKR--TSRSFKGKDDFI 387

Query: 379 NEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEIPFPHRAGNLYKIQHLVY 438
            EPIPK  ++ +W+ F   +A  A++IL+P+GGKM+EI+++EIPFPHR GNLY+IQ+L Y
Sbjct: 388 EEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAY 447

Query: 439 WEEE---GEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIGTN-NKNGNTSYGE 498
           W EE    +   ++++ W+  +Y ++ P+VSK+PR AY+N+RD+D+G     N  T Y E
Sbjct: 448 WSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEE 507

Query: 499 ASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
           A +WGVKYFK NF RLV VKT +DP +FF +EQSIP
Sbjct: 508 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0305 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 4.9e-155
Identity = 275/534 (51.50%), Postives = 364/534 (68.16%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60
           MK  + L+ +  V ++ S++S+ N E F  CL+ +S     IS  I+   N SYSSVL  
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQA 60

Query: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYV--SD 120
           +  NLRF T+ TPKP +I+     S VQA+I C K +  Q++ RSGGHDY+GLSYV  S 
Sbjct: 61  NIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 120

Query: 121 VSFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 180
             F V+D+ NLRS+++DV  K AWVQ+GA +GE+YY I EKSKTL +PAG+CPTVGVGGH
Sbjct: 121 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 180

Query: 181 FSGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAW 240
            SGGGYG M+RK+GL  DN +DA  +DVNGK+ DRK MGED++WA+ GGGG S+G+VLA+
Sbjct: 181 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 240

Query: 241 KIKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEK 300
           KI L+ VP  VTVF ++R LEQNATD++HRWQ VA K  D+LFIR ++  VN T     +
Sbjct: 241 KINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGT--VSSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           +T+   F ++FLG    LLSI+++ FP LGL + DC + SWI+S+L++     G    +L
Sbjct: 301 KTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           L R    P  + K KSDYV EPI + GLE IWK   E   E   M  +PYGG+M  IS +
Sbjct: 361 LQRNQ--PVNYLKRKSDYVREPISRTGLESIWKKMIE--LEIPTMAFNPYGGEMGRISST 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
             PFP+RAGNL+KIQ+   W +  E +  R++   RKLY ++ PFVSKNPR ++ NYRD+
Sbjct: 421 VTPFPYRAGNLWKIQYGANWRD--ETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDV 480

Query: 481 DIGTNNKNGN-TSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSL 532
           D+G N+ NG  +SY E   +G KYF GNF+RLV +KT++D  NFFRNEQSIP L
Sbjct: 481 DLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of MC03g_new0305 vs. NCBI nr
Match: XP_022152432.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60
           MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF
Sbjct: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60

Query: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120
           STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS
Sbjct: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120

Query: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180
           FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS
Sbjct: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180

Query: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 240
           GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI
Sbjct: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 240

Query: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 300
           KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT
Sbjct: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 300

Query: 301 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 360
           IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD
Sbjct: 301 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 360

Query: 361 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 420
           RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI
Sbjct: 361 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 420

Query: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480
           PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI
Sbjct: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480

Query: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL 540
           GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL
Sbjct: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL 540

BLAST of MC03g_new0305 vs. NCBI nr
Match: XP_004135722.1 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653302.1 hypothetical protein Csa_023170 [Cucumis sativus])

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 457/542 (84.32%), Postives = 497/542 (91.70%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MKS IT  A+ F+VL F  AASAHNHE FLHCLS HSPN SSIS IIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFAHIFIVLTFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKPQVIVTP  VSQ+QASI+CAKN GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RG GGAS+GIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVPP+VTVFT+ R LEQNATD++HRWQYV+ KQDDKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFP LGL KEDCI+MSWIES+LYFAGF RG+ L VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYVNEPIPK GLEGIW+ FNEE+AEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEK 539
           DIGTN KNG NTSY EAS+WG+KYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGIKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MC03g_new0305 vs. NCBI nr
Match: XP_038895082.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 462/542 (85.24%), Postives = 497/542 (91.70%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MKS IT  A  F+VL F  AASA NHE FLHCLSLH PN SSIS IIYTPTNPSYSSVLN
Sbjct: 1   MKSQITSFACIFIVLSFAWAASADNHEAFLHCLSLHYPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKPQVI+TP QV QVQASI+CAKN GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPQVIITPFQVFQVQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            F+VVDLINLRSI++DVE   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFVVVDLINLRSISVDVENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDV+GKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVDGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVPP VTVFTVTRNLEQNAT+L+++WQY++ KQD+ LFIRIILRG+N+T+ Q G K
Sbjct: 241 IKLLPVPPTVTVFTVTRNLEQNATNLINQWQYISSKQDENLFIRIILRGINTTNSQDGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RTIEAAFNSLFLGK+E+LL IM +TFP LGL KEDCI+MSWIESILYFAGF RG+ L VL
Sbjct: 301 RTIEAAFNSLFLGKIEQLLQIMQETFPELGLTKEDCIEMSWIESILYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYVNEPIPK GLEGIW+LFNEE+AEAAEMILSPYGG M++ISES
Sbjct: 361 LDRRPLFPKRFFKAKSDYVNEPIPKAGLEGIWELFNEEEAEAAEMILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHR GNLYKIQHLVYWEEEGED+AQRHINWIRKLYSY+APFVSKNPR AYINYRDL
Sbjct: 421 EIPFPHREGNLYKIQHLVYWEEEGEDIAQRHINWIRKLYSYMAPFVSKNPRLAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEK 539
           DIG N KNG NTSY EAS+WGVKYFKGNFKRLVSVKTKIDPSNFF+NEQSIPSLL WW+K
Sbjct: 481 DIGMNEKNGRNTSYSEASVWGVKYFKGNFKRLVSVKTKIDPSNFFKNEQSIPSLLPWWKK 540

BLAST of MC03g_new0305 vs. NCBI nr
Match: XP_008445653.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047121.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK24891.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 457/542 (84.32%), Postives = 496/542 (91.51%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MKS IT   + F+VL F  AASAHNHE FLHCLS HSPN SSIS IIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKP +IVTP  VSQ+QASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVP +VTVFT+TR LEQNATDL+HRWQYV+ KQDDKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFP LGL KEDCI+MSWIES+LYFAGF RG+ L VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYVNEPIPK GLEGIW+LF+EE+AEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEK 539
           DIGTN KNG NTSY EAS+WGVKYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MC03g_new0305 vs. NCBI nr
Match: XP_022954564.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 932 bits (2410), Expect = 0.0
Identity = 454/541 (83.92%), Postives = 492/541 (90.94%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MK PITL  YTFVVL F  AASAHNHEDFLHCLSLHSPN SSIS IIYTP + SYSSVLN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRF T +TPKPQVIVTPLQV++++ASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            FIVVDLINLRSIN+D E   AWVQSGATIGELYYRIA+KSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNV+DAYFIDVNG LHDRKSMGEDVFWA+RGGGGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLL VPP+VTVFTVTRNLEQNAT+L+HRWQY+A KQDD+LFIRII+RGVNST+ Q+G +
Sbjct: 241 IKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RT+EAAFNS+FLGK E+LL IM  TFP LGL KEDCI+MSWIESILYFAGFP G+ L VL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDR PL PKRFFKAKSDYVNEPIPK  LEG W+LFNEE+AEAAEMILSPYGG M+EISES
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYWEEEGED+A+RHINWIRKLYS+V P+VSKNPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKR 539
           DIG NNKNG TSY EAS+WGVKYFKGNFKRLVS+KTK+DPSNFF NEQSIPS+L  W+KR
Sbjct: 481 DIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWKKR 540

BLAST of MC03g_new0305 vs. ExPASy TrEMBL
Match: A0A6J1DG91 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020167 PE=3 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60
           MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF
Sbjct: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60

Query: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120
           STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS
Sbjct: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120

Query: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180
           FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS
Sbjct: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180

Query: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 240
           GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI
Sbjct: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 240

Query: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 300
           KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT
Sbjct: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 300

Query: 301 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 360
           IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD
Sbjct: 301 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 360

Query: 361 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 420
           RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI
Sbjct: 361 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 420

Query: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480
           PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI
Sbjct: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480

Query: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL 540
           GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL
Sbjct: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL 540

BLAST of MC03g_new0305 vs. ExPASy TrEMBL
Match: A0A5A7U0J8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5482G00070 PE=3 SV=1)

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 457/542 (84.32%), Postives = 496/542 (91.51%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MKS IT   + F+VL F  AASAHNHE FLHCLS HSPN SSIS IIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKP +IVTP  VSQ+QASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVP +VTVFT+TR LEQNATDL+HRWQYV+ KQDDKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFP LGL KEDCI+MSWIES+LYFAGF RG+ L VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYVNEPIPK GLEGIW+LF+EE+AEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEK 539
           DIGTN KNG NTSY EAS+WGVKYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MC03g_new0305 vs. ExPASy TrEMBL
Match: A0A1S3BE31 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488607 PE=3 SV=1)

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 457/542 (84.32%), Postives = 496/542 (91.51%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MKS IT   + F+VL F  AASAHNHE FLHCLS HSPN SSIS IIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKP +IVTP  VSQ+QASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVP +VTVFT+TR LEQNATDL+HRWQYV+ KQDDKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFP LGL KEDCI+MSWIES+LYFAGF RG+ L VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYVNEPIPK GLEGIW+LF+EE+AEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEK 539
           DIGTN KNG NTSY EAS+WGVKYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MC03g_new0305 vs. ExPASy TrEMBL
Match: A0A6J1GTC1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE=3 SV=1)

HSP 1 Score: 932 bits (2410), Expect = 0.0
Identity = 454/541 (83.92%), Postives = 492/541 (90.94%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MK PITL  YTFVVL F  AASAHNHEDFLHCLSLHSPN SSIS IIYTP + SYSSVLN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRF T +TPKPQVIVTPLQV++++ASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            FIVVDLINLRSIN+D E   AWVQSGATIGELYYRIA+KSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNV+DAYFIDVNG LHDRKSMGEDVFWA+RGGGGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLL VPP+VTVFTVTRNLEQNAT+L+HRWQY+A KQDD+LFIRII+RGVNST+ Q+G +
Sbjct: 241 IKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RT+EAAFNS+FLGK E+LL IM  TFP LGL KEDCI+MSWIESILYFAGFP G+ L VL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDR PL PKRFFKAKSDYVNEPIPK  LEG W+LFNEE+AEAAEMILSPYGG M+EISES
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYWEEEGED+A+RHINWIRKLYS+V P+VSKNPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKR 539
           DIG NNKNG TSY EAS+WGVKYFKGNFKRLVS+KTK+DPSNFF NEQSIPS+L  W+KR
Sbjct: 481 DIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWKKR 540

BLAST of MC03g_new0305 vs. ExPASy TrEMBL
Match: A0A6J1K043 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3 SV=1)

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 452/540 (83.70%), Postives = 487/540 (90.19%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLN 60
           MKSPITL  YTFVVL F  AASAHNHEDFLHCLSLHSPN SSIS  IYTP + SYSSVLN
Sbjct: 1   MKSPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKTIYTPNDSSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRF T  TPKPQVIVTPLQVS+++ASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPRTPKPQVIVTPLQVSEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            FI+VDLINLRSIN+D +   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIIVDLINLRSINVDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNV+DAYFIDVNG LHDRKSMGEDVFWA+RGGGGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD-QYGEK 300
           IKLL VPP+VTVFTV R LEQNAT+L+HRWQY+A KQDD+LFIRII+RGVNST+ Q G +
Sbjct: 241 IKLLQVPPIVTVFTVPRTLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQQGSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           RT+EAAFNS+FLGK E+LL IM  TFP LGL KEDCI+MSWIESILYFAGFP G+ L VL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           LDR PL PKRFFKAKSDYVNEPIPK  LEG W+LF EE+AEAAEMILSPYGG M+EISES
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFKEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYWEEEGED+A+RHINWIRKLYSYVAP+VS NPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSYVAPYVSNNPRAAYINYRDL 480

Query: 481 DIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKR 538
           DIG NNKNG TSY EAS+WGVKYFKGNFKRLVS+KTK+DPSNFF NEQSIPS+L WW+KR
Sbjct: 481 DIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPWWKKR 540

BLAST of MC03g_new0305 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 559.7 bits (1441), Expect = 2.6e-159
Identity = 276/533 (51.78%), Postives = 372/533 (69.79%), Query Frame = 0

Query: 2   KSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPS--YSSVLN 61
           K   TL   T +++ V  +S+   +DF+ CL  +S     I+   ++P   +  +   L 
Sbjct: 7   KRNATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELE 66

Query: 62  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-- 121
            +  NLR+ T   PKP  I  PL  + VQA++VCAK     +R RSGGHDYEGLS+V+  
Sbjct: 67  STAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAED 126

Query: 122 DVSFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGG 181
           +  F++VDL  LR +++D++   AW  +GATIGE+YYRI EKS+T GFPAG+C ++G+GG
Sbjct: 127 ETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGG 186

Query: 182 HFSGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLA 241
           H  GG YG M+RKFGL ADNV+DA  +D NG++ DR +MGEDVFWA+RGGGG SFG++LA
Sbjct: 187 HLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILA 246

Query: 242 WKIKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGE 301
           WKIKL+PVP  VTVFTVT+ LEQ+ T ++++W+ +ADK DD LFIR+I+   + T + G 
Sbjct: 247 WKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG- 306

Query: 302 KRTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEV 361
            RTI  ++ + FLG   +LL +M K+FP LGL K+DC +MSWI+S++Y AGFP     E 
Sbjct: 307 NRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEA 366

Query: 362 LLDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISE 421
           LL  + L  K  FKAKSD+V EPIP  GLEG+W+ F EED+     I +PYGG M+ ISE
Sbjct: 367 LLAGKSLF-KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISE 426

Query: 422 SEIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRD 481
           SEIPFPHR G L+KIQ L  W ++G+   +RH+ WIR++YSY+  +VSKNPR AY+NYRD
Sbjct: 427 SEIPFPHRNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRD 486

Query: 482 LDIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPS 531
           LD+GTN   G T   +A  WG KY+KGNF+RLV +K + DP NFFR+EQS+P+
Sbjct: 487 LDLGTN--EGET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of MC03g_new0305 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 555.8 bits (1431), Expect = 3.7e-158
Identity = 273/528 (51.70%), Postives = 374/528 (70.83%), Query Frame = 0

Query: 7   LLAYTFVVLFVS-----AASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNFS 66
           L+ +T   L +S     +A+  N   FL CLSL   + + +S +I+TP + S+SSVL  S
Sbjct: 11  LIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASS 70

Query: 67  THNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVSF 126
             N RF+  + PKP +I+TP+Q S VQ+++ CA+  G  IRTRSGGHDYEGLSYV+   F
Sbjct: 71  IQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPF 130

Query: 127 IVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSG 186
           +++DL NLRSI +DV+ +  WVQ+GATIGELYY I +K++TL FPAGVCPTVGVGGHFSG
Sbjct: 131 VILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSG 190

Query: 187 GGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKIK 246
           GGYG +LRK GLAAD+V+DA  +D  G++ +R+ MGED FWA+RGGGG+SF +VL+WKI 
Sbjct: 191 GGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIG 250

Query: 247 LLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRTI 306
           L+ VP  VTVF VT+  EQ+A  ++HRWQ+VADK  D LFIR++L+      +Y  K  +
Sbjct: 251 LINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQ------RY--KNMV 310

Query: 307 EAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLDR 366
            A+F  L+LG V+ LL +++K FP LGL ++DC +MSWIES+++FA     E + VL  R
Sbjct: 311 RASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGE-EPINVLTKR 370

Query: 367 RPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEIP 426
                   FKAKSD+V EP+PK  +  +W+   E +AE A++I +P+GGKM+EI++ E P
Sbjct: 371 TR--ASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETP 430

Query: 427 FPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIG 486
           FPHR GN+Y+IQ+L YW     DV ++++ W+ ++Y  ++ FV+K+PR AYIN RDLD+G
Sbjct: 431 FPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLG 490

Query: 487 TNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
                  + Y E   WGVKYFK NF+RLV VKT +DPS+FF +EQSIP
Sbjct: 491 MYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MC03g_new0305 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.4e-157
Identity = 272/532 (51.13%), Postives = 372/532 (69.92%), Query Frame = 0

Query: 5   ITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTP--TNPSYSSVLNFST 64
           + LL+ +++ L  S       +DF+ CL  +S     + +  +T      S+   L  S 
Sbjct: 16  LLLLSLSYIPLSFSTI----QQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 75

Query: 65  HNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV--S 124
            NLR+     PKP+ I  PL  + VQA+++CAK     +R RSGGHDYEGLSYVS++  +
Sbjct: 76  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 135

Query: 125 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 184
           F++VDL  LR I++D+E   AWV +GA+IGE+YYRI EKSK  GFPAG+C ++G+GGH  
Sbjct: 136 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 195

Query: 185 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 244
           GG YG M+RKFGL ADNV+DA  +D +GK+ +R +MGEDVFWA+RGGGG SFG++LAWKI
Sbjct: 196 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 255

Query: 245 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 304
           KL+PVP +VTVFTVTR LEQ+ T L+++WQ VADK D+ LFIR+I++  + T +  ++RT
Sbjct: 256 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPK-SKERT 315

Query: 305 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 364
           I  ++   FLG   +LL +M ++FP LGL K+DC++ SWI+S++Y AGFP     E LLD
Sbjct: 316 ISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD 375

Query: 365 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 424
            + L  K +FKAKSDYV EPIP  GLEG+W+   EED+     I +PYGG M +I E+E 
Sbjct: 376 GKSLF-KNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETET 435

Query: 425 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 484
           PFPHR+G L+KIQ L  W ++G+    +H+ W+R++YSY+  +VSK+PR+AY+NYRDLD+
Sbjct: 436 PFPHRSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDL 495

Query: 485 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLL 533
           G N K       +A  WG +YFKGNF+RLV +K K DP NFFR+EQSIP+ L
Sbjct: 496 GMNGKG-----SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of MC03g_new0305 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 550.4 bits (1417), Expect = 1.6e-156
Identity = 269/516 (52.13%), Postives = 366/516 (70.93%), Query Frame = 0

Query: 19  AASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNFSTHNLRFTTSETPKPQVI 78
           +A   NHE+FL CLS H  N    S II+T  +PSY S+LN S  N RF   ETPKP  I
Sbjct: 28  SAQRTNHENFLKCLS-HRINEDD-SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSI 87

Query: 79  VTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-DVSFIVVDLINLRSINIDVE 138
           +TP+Q + VQ++I CA+ +G  IRTRSGGHDYEGLSY++    F+V+DL NLRSI +DV+
Sbjct: 88  ITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVD 147

Query: 139 KKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADN 198
            +  WVQSGATIGELYY I + SK+L FPAG+ PTVG+GG F GGGYG ++RK+GL+ADN
Sbjct: 148 NRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADN 207

Query: 199 VVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKIKLLPVPPVVTVFTVTRN 258
           V+DA+ +D NG   DR+ MGED FWA+RGGGG+SF +VL+WKI+LL VP VVTVF V + 
Sbjct: 208 VIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKT 267

Query: 259 LEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRTIEAAFNSLFLGKVEKLL 318
            E+ A  ++++WQY+ADK  + LFIR +L+         ++  + A+F  L+LG V  LL
Sbjct: 268 SEKEAVSIINKWQYIADKVPNDLFIRAMLQ---------KETEVYASFPGLYLGPVSDLL 327

Query: 319 SIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLDRRPLVPKRFFKAKSDYV 378
           ++M   FP LGL   +C +MSWIES+L+F    +GE +E+L  R+     R FK K D++
Sbjct: 328 ALMKDKFPELGLEIGNCREMSWIESVLWFI---KGESMEILAKRKR--TSRSFKGKDDFI 387

Query: 379 NEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEIPFPHRAGNLYKIQHLVY 438
            EPIPK  ++ +W+ F   +A  A++IL+P+GGKM+EI+++EIPFPHR GNLY+IQ+L Y
Sbjct: 388 EEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAY 447

Query: 439 WEEE---GEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIGTN-NKNGNTSYGE 498
           W EE    +   ++++ W+  +Y ++ P+VSK+PR AY+N+RD+D+G     N  T Y E
Sbjct: 448 WSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEE 507

Query: 499 ASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
           A +WGVKYFK NF RLV VKT +DP +FF +EQSIP
Sbjct: 508 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0305 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 549.3 bits (1414), Expect = 3.5e-156
Identity = 275/534 (51.50%), Postives = 364/534 (68.16%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60
           MK  + L+ +  V ++ S++S+ N E F  CL+ +S     IS  I+   N SYSSVL  
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQA 60

Query: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYV--SD 120
           +  NLRF T+ TPKP +I+     S VQA+I C K +  Q++ RSGGHDY+GLSYV  S 
Sbjct: 61  NIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 120

Query: 121 VSFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 180
             F V+D+ NLRS+++DV  K AWVQ+GA +GE+YY I EKSKTL +PAG+CPTVGVGGH
Sbjct: 121 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 180

Query: 181 FSGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAW 240
            SGGGYG M+RK+GL  DN +DA  +DVNGK+ DRK MGED++WA+ GGGG S+G+VLA+
Sbjct: 181 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 240

Query: 241 KIKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEK 300
           KI L+ VP  VTVF ++R LEQNATD++HRWQ VA K  D+LFIR ++  VN T     +
Sbjct: 241 KINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGT--VSSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVL 360
           +T+   F ++FLG    LLSI+++ FP LGL + DC + SWI+S+L++     G    +L
Sbjct: 301 KTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLL 360

Query: 361 LDRRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISES 420
           L R    P  + K KSDYV EPI + GLE IWK   E   E   M  +PYGG+M  IS +
Sbjct: 361 LQRNQ--PVNYLKRKSDYVREPISRTGLESIWKKMIE--LEIPTMAFNPYGGEMGRISST 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
             PFP+RAGNL+KIQ+   W +  E +  R++   RKLY ++ PFVSKNPR ++ NYRD+
Sbjct: 421 VTPFPYRAGNLWKIQYGANWRD--ETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDV 480

Query: 481 DIGTNNKNGN-TSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSL 532
           D+G N+ NG  +SY E   +G KYF GNF+RLV +KT++D  NFFRNEQSIP L
Sbjct: 481 DLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526