MC03g_new0304 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0304 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 4.9e-158
Identity = 281/537 (52.33%), Postives = 376/537 (70.02%), Query Frame = 0

Query: 20  SLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFS--------- 79
           +L  + ++L  V ++S++  +DF++CL     +NS +S     PI  S+FS         
Sbjct: 11  TLFLVTLLLISVPLSSSTLQQDFVKCL----VDNSDVS----FPITASFFSPDQNATLFK 70

Query: 80  -ILNFSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSY 139
             L  + QNLR+ T   PKP  I  PL  +H+QA+++CAK     +R RSGGHDYEGLS+
Sbjct: 71  EELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSF 130

Query: 140 VS-DNVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTV 199
           V+ D  PFV++DL+ LR +DVD++ N AW  +GATIGE+YYRI EKS+T GFPAGLCS++
Sbjct: 131 VAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSL 190

Query: 200 GVGGHFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFG 259
           G+GGH  GG YG M+RKFGL ADNV+DA  +D NG + DR +MGEDVFWAIRGGGG SFG
Sbjct: 191 GIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFG 250

Query: 260 IILAWKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSS 319
           +ILAWKIKL+PVP  VTVFT+T+ LEQ+ T ++++W+ +A K DD L IRVI++    ++
Sbjct: 251 VILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTT 310

Query: 320 NNQNQKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF--Q 379
              N  +T+  S+ + FLG  + LL +MQK+FPELGLTK+DC EMSWI++V+Y AGF   
Sbjct: 311 KPGN--RTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNS 370

Query: 380 GQPLNVLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKM 439
             P  +L  +SL +K  FK KSD+V EPIP  G+EG+WE F EE+      +  PYGG M
Sbjct: 371 AAPEALLAGKSL-FKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMM 430

Query: 440 SEISESEIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYI 499
           S ISESEIPFPHR G L+KI +     +G+ + +R + WIR++YSYM  +VSKNPR AY+
Sbjct: 431 SRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYV 490

Query: 500 NYRDLDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPS 544
           NYRDLD+GTN  +G T    A  WG KY+K NF+RLV +K + DP NFFR+EQS+P+
Sbjct: 491 NYRDLDLGTN--EGETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of MC03g_new0304 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 6.4e-158
Identity = 279/530 (52.64%), Postives = 363/530 (68.49%), Query Frame = 0

Query: 24  IFVVLSF-VWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILNFSIQNLRFA 83
           IF  LSF + ++ A+  E+FL+C S H PNN +  K+VYT  D  Y SILN +IQNLRF 
Sbjct: 15  IFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFI 74

Query: 84  TAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVPFVVIDLT 143
           +  TPKP VIVTP   SHIQA+I+C+K  G QIRTRSGGHD EG+SY+S  VPFVV+DL 
Sbjct: 75  SDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYIS-QVPFVVVDLR 134

Query: 144 NLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYGVM 203
           N+ SI +DV    AWV++GAT+GE+YY I EK++ L FP G C TVGVGGHFSGGGYG +
Sbjct: 135 NMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGAL 194

Query: 204 LRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPVPP 263
           +R +GLAADN+IDA+ ++V+G + DRKSMGED+FWAIRGGGG +FGII AWKIKL+ VP 
Sbjct: 195 MRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPS 254

Query: 264 VVTVFTITRNLE-QNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQKKTLEASF 323
             T+F++ +N+E      L ++WQ +A K D  L++         + N+   K T+   F
Sbjct: 255 KSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYF 314

Query: 324 NSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-----QGQPLNVLLDR 383
           +S+F G VD L+ +M K+FPELG+ K DC E SWI+  ++++G            +LLDR
Sbjct: 315 SSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDR 374

Query: 384 SLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESEIPF 443
           S   K  F  K DYV +PIP+  +  I E   EE+V     +L PYGG M EISES IPF
Sbjct: 375 SAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPF 434

Query: 444 PHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTN 503
           PHR G +Y++ Y     + ED  ++ I+W+R +Y++ TP+VS+NPR AY+NYRDLD+G  
Sbjct: 435 PHRAGIMYELWYTASWEKQEDN-EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKT 494

Query: 504 NKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSP 547
           N     +Y +A IWG KYF  NF RLV VKTKVDP+NFFRNEQSIP L P
Sbjct: 495 NHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of MC03g_new0304 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 8.3e-158
Identity = 273/515 (53.01%), Postives = 368/515 (71.46%), Query Frame = 0

Query: 34  ASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILNFSIQNLRFATAKTPKPQVIV 93
           A  +N+E+FL+CLS     N   S+I++T  D SYFSILN SIQN RF   +TPKP  I+
Sbjct: 29  AQRTNHENFLKCLSHRI--NEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 94  TPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVPFVVIDLTNLRSIDVDVEK 153
           TP+Q + +Q++I CA+ +G  IRTRSGGHDYEGLSY++ + PFVVIDL NLRSI +DV+ 
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 154 NVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYGVMLRKFGLAADNV 213
              WVQSGATIGELYY I + SK+L FPAGL  TVG+GG F GGGYG ++RK+GL+ADNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 214 IDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPVPPVVTVFTITRNL 273
           IDA+ +D NG+  DR+ MGED FWAIRGGGG+SF ++L+WKI+LL VP VVTVF + +  
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 274 EQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQKKT-LEASFNSLFLGQVDEL 333
           E+ A ++I++WQY+A K  + L IR +L           QK+T + ASF  L+LG V +L
Sbjct: 269 EKEAVSIINKWQYIADKVPNDLFIRAML-----------QKETEVYASFPGLYLGPVSDL 328

Query: 334 LPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVLLDRSLAWKGTFKGKSDYVN 393
           L +M+  FPELGL   +C EMSWIE+VL+F   +G+ + +L  R    + +FKGK D++ 
Sbjct: 329 LALMKDKFPELGLEIGNCREMSWIESVLWF--IKGESMEILAKRKRTSR-SFKGKDDFIE 388

Query: 394 EPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESEIPFPHRVGNLYKIHYAVLL 453
           EPIPK  I+ +W  F   E + A+++L P+GGKMSEI+++EIPFPHR GNLY+I Y    
Sbjct: 389 EPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYW 448

Query: 454 GEGED----TAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTN-NKDGNTSYDKA 513
            E ED      ++ + W+  +Y +MTP+VSK+PR AY+N+RD+D+G     +  T Y++A
Sbjct: 449 SEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEA 508

Query: 514 SIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIP 543
            +WG+KYFK+NF RLV VKT VDP +FF +EQSIP
Sbjct: 509 KVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0304 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.8e-157
Identity = 273/530 (51.51%), Postives = 361/530 (68.11%), Query Frame = 0

Query: 24  IFVVLSF-VWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILNFSIQNLRFA 83
           IF  LSF + ++ A+  E+FL+C S + PNN +  K +YT  D  Y S+LN +IQNLRF 
Sbjct: 15  IFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFT 74

Query: 84  TAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVPFVVIDLT 143
           +  TPKP VIVTP  VSHIQASI+C+K  G QIRTRSGGHD EGLSY+S  VPF ++DL 
Sbjct: 75  SDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYIS-QVPFAIVDLR 134

Query: 144 NLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYGVM 203
           N+ ++ VD+    AWV++GAT+GE+YY I E ++   FP G C TVGVGGHFSGGGYG +
Sbjct: 135 NMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGAL 194

Query: 204 LRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPVPP 263
           +R +GLAADN+IDA+ ++V+G + DRKSMGED+FWAIRGGGG +FGII AWKIKL+ VP 
Sbjct: 195 MRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPS 254

Query: 264 VVTVFTITRNLE-QNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQKKTLEASF 323
             T+F++ +N+E      L ++WQ +A K D  L++         + N+   K T+   F
Sbjct: 255 KATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYF 314

Query: 324 NSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQ-----PLNVLLDR 383
           +S+FLG VD L+ +M K+FPELG+ K DC E+SWI+  ++++G            +LLDR
Sbjct: 315 SSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDR 374

Query: 384 SLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESEIPF 443
           S   K  F  K DYV + IP+  +  I E   EEEV     +L PYGG M EISES IPF
Sbjct: 375 SAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPF 434

Query: 444 PHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTN 503
           PHR G +Y++ Y     + ED  ++ I+W+R +Y++ TP+VS+NPR AY+NYRDLD+G  
Sbjct: 435 PHRAGIMYELWYTATWEKQEDN-EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKT 494

Query: 504 NKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSP 547
           N +   +Y +A IWG KYF  NF RLV VKTK DP+NFFRNEQSIP L P
Sbjct: 495 NPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of MC03g_new0304 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.0e-157
Identity = 268/532 (50.38%), Postives = 369/532 (69.36%), Query Frame = 0

Query: 22  AYIFVVLSF------VWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILNFS 81
           +++F +L F      +  A  SN+EDFL+CLS+   +N+   K+++T  D S+FSIL+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 82  IQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVP 141
           IQN RF+ ++TPKP  I+TP++ S +Q  I CA+ +G  +RTRS GH YEGLSY++ N P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 142 FVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFS 201
           F VIDL NLRSI +DV+    WVQ+GAT GELYY I + +K+L FPAG+  TVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 202 GGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKI 261
           GGGYG +LRK+GLAADN+IDA  +D +G + DR++MGED FWAIRGGGG+SFG+IL+WK+
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 262 KLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQKK 321
           KL+ VP  +TVF + +  ++ A  +I +WQY A K  D L IR  L         ++ K 
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL--------ERSNKN 304

Query: 322 TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNVLL 381
            + A F  L++G V+ LL +M++ FPELGL KE C EMSWIE+VL+FA F +G+ L VL 
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 364

Query: 382 DRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESEI 441
           +R      +FKGK D+V EPIP+A I+ IW      E +  +++L P+GGKMSE++E E 
Sbjct: 365 NRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424

Query: 442 PFPHRVGNLYKIHYAVLLGEGED----TAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 501
           PFPHR GNLY+I Y     E ED       + + W+  +Y +MTP+VSK+PR AY+N++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 502 LDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIP 543
           +D+G       T Y++   WG+KYFK+NF+RLV VKT+VDP++FF +EQSIP
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MC03g_new0304 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 1087 bits (2812), Expect = 0.0
Identity = 538/540 (99.63%), Postives = 538/540 (99.63%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQ 313
           KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 314 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVL 373
           KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVL
Sbjct: 301 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVL 360

Query: 374 LDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESE 433
           LDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE E
Sbjct: 361 LDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEXE 420

Query: 434 IPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDI 493
           IPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDI
Sbjct: 421 IPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDI 480

Query: 494 GTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH 553
           GTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH
Sbjct: 481 GTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH 540

BLAST of MC03g_new0304 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 444/541 (82.07%), Postives = 486/541 (89.83%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPI SLAYIFVVLSF  VASA NYEDFL CLSFH PN+SSISKI+YTP   SY  +LN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSIQNLRF  AKTPKPQ+IVTPL+ SH+QASIICAK+NG QIRTRSGGHDYEGLSYVS N
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVS-N 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPFVV+DLTNLRSIDVDVE+ VAWVQSGATIGELYYRIAEKSKTLGFPAGLC TVG+GGH
Sbjct: 121 VPFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYGVMLRKFGLAADNVIDAYFIDVNG L DRKSMGEDVFWAIRGGGGASFGI+LAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQ 313
           KIKLL VP +VTVFTITRNLEQNATNLI++WQYVA+KQDDRL+I++IL G  NS+N++N 
Sbjct: 241 KIKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGT-NSNNHRNG 300

Query: 314 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNV 373
           K+T+EA FNSLFLG+V++LLPIMQKTFPELGL KEDCIEMSWIE+VLYFA F +GQPLNV
Sbjct: 301 KRTIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNV 360

Query: 374 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISES 433
           LLDR   +KG+FKGKSDYVNEP+PKAG+EGIWELFNE+E +  QM LIPYGGKM+EISES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 434 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 493
           EIPFPHR GNLY+IHY++  G  E+  QR   WIRK YSYM PFVSKNPRAAYINYRDLD
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLD 480

Query: 494 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 553
           IGTNN++GNTSYDKASIWG+KYFK NFKRLVSVK KVDPSNFFRNEQSIPS   WWKK G
Sbjct: 481 IGTNNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPSR--WWKKGG 535

BLAST of MC03g_new0304 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 882 bits (2280), Expect = 0.0
Identity = 431/541 (79.67%), Postives = 484/541 (89.46%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPITSLAYIF+ LS  WVASA N+EDFL CLS HSPN+S IS I+YTP D SY S+LN
Sbjct: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSIQN RF  AKTPKPQ+IVTPL+ SHIQAS+ICAK+NGFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSD- 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPFV++DLTN+RSIDVDVE+NVAWVQSGATIGELYYRIAEKSKTLGFPAGLC TVG+GGH
Sbjct: 121 VPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYG+MLRK+GLAADNVIDAYFIDVNG L DRKSMGEDVFWAIRG GGASFGIILAW
Sbjct: 181 FSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRG-GGASFGIILAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQ 313
           K+KL+ VPPVVTVF+ITRNL+QNATNL+H+WQYVA+KQDDRL+IR +L G+ NS N+QN 
Sbjct: 241 KVKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVL-GVTNSKNHQNG 300

Query: 314 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNV 373
           K+T++A+FNSLFLG+V ELLPIMQ TFPELGL KEDCIEMSWIE+VLYF+ F + QPLNV
Sbjct: 301 KRTVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNV 360

Query: 374 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISES 433
           L+DR+LA KG FKGKSDY+N+PIPKAG+EG+WEL   +EV+F +M L+PYGGKM+EISE+
Sbjct: 361 LIDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEA 420

Query: 434 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 493
           +IPFPHR  NLY IHY V   E  D+ QR ISWIRKLY YMTPFVSKNPRAAY NYRDLD
Sbjct: 421 DIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLD 480

Query: 494 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 553
           IGTNNKDGNT+Y+KASIWG KY+K NFKRLVS+KTKVDPSNFFRNEQSIPSL PWWK  G
Sbjct: 481 IGTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGG 537

BLAST of MC03g_new0304 vs. NCBI nr
Match: XP_022152545.1 (berberine bridge enzyme-like 13 [Momordica charantia])

HSP 1 Score: 868 bits (2243), Expect = 4.65e-314
Identity = 425/543 (78.27%), Postives = 481/543 (88.58%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPITSLAY FVV+SF+WVASASNYEDFL CLS HSPN+SSISKI+YTP D SY  I+N
Sbjct: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVS-D 133
           FS  NL   TAK+ KPQ IVTPLQVSHIQ SIICAK +GFQIRTRSGGHDYE LS +S D
Sbjct: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQXSIICAKKHGFQIRTRSGGHDYERLSSISYD 120

Query: 134 NVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGG 193
           ++PFVV+D+ N+RSIDV+VEKNVAWVQSGATIGELYYRIA+KSKTLGFPAGLC TVGVGG
Sbjct: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180

Query: 194 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILA 253
           HFSGGGYG+MLRKFGLAA+NVIDAYF+DVNG L +RKS GEDVFWAIRGGGGASF II+A
Sbjct: 181 HFSGGGYGMMLRKFGLAABNVIDAYFVDVNGKLHNRKSXGEDVFWAIRGGGGASFXIIVA 240

Query: 254 WKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 313
           WKIKL+  PPVVTVF ITRNLEQNATNL+H+WQYVA+KQDD+LLI++ L GM NS+N+QN
Sbjct: 241 WKIKLVQXPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGM-NSNNHQN 300

Query: 314 QKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQG-QPLN 373
            K+T E SF+SLFLGQ +ELLPIMQ TFPELGLTKEDCIEMSWIE+VL+FAGF+G Q L+
Sbjct: 301 GKRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQXLD 360

Query: 374 VLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE 433
           VLLDR++A++   K K+DYVNEPIP  G++GIWELFNEEEV+FAQ+  IPYGGKM+EI E
Sbjct: 361 VLLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPE 420

Query: 434 SEIPFPHRVGNLYKIHYAVL-LGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 493
           SEIPFPHR GNLYKIHY+V+   E EDT Q+ I+WIRKL SYMTPFVSKNPRAAYINYRD
Sbjct: 421 SEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRD 480

Query: 494 LDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKK 553
           LDIG NNK+G TSY+KASIWG++YFK NFKRLVS+KTKVDP NFFR+EQSIPSL PWW K
Sbjct: 481 LDIGINNKNGYTSYEKASIWGLRYFKGNFKRLVSIKTKVDPYNFFRHEQSIPSLLPWWTK 540

BLAST of MC03g_new0304 vs. NCBI nr
Match: XP_022954564.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 866 bits (2237), Expect = 3.37e-313
Identity = 427/544 (78.49%), Postives = 479/544 (88.05%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MK PIT   Y FVVLSF W ASA N+EDFL CLS HSPN+SSISKI+YTP D SY S+LN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSI NLRFAT +TPKPQVIVTPLQV+ I+ASIICAK+ GFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSD- 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPF+V+DL NLRSI+VD E NVAWVQSGATIGELYYRIA+KSKTLGFPAG+C TVGVGGH
Sbjct: 121 VPFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYG+MLRKFGLA DNVIDAYFIDVNGNL DRKSMGEDVFWA+RGGGGASFGI++AW
Sbjct: 181 FSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN- 313
           KIKLL VPP+VTVFT+TRNLEQNATNLIH+WQY+ASKQDDRL IR+I+ G +NS+N Q+ 
Sbjct: 241 KIKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRG-VNSTNPQHG 300

Query: 314 QKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLN 373
            ++TLEA+FNS+FLG+ +ELLPIMQ TFPELGLTKEDCIEMSWIE++LYFAGF  GQPLN
Sbjct: 301 SQRTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLN 360

Query: 374 VLLDRS-LAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEIS 433
           VLLDR+ L  K  FK KSDYVNEPIPKA +EG WELFNEEE + A+M+L PYGG M EIS
Sbjct: 361 VLLDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEIS 420

Query: 434 ESEIPFPHRVGNLYKIHYAVLLGE-GEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYR 493
           ESE PFPHR GNLYKI + V   E GED A+R I+WIRKLYS++ P+VSKNPRAAYINYR
Sbjct: 421 ESETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYR 480

Query: 494 DLDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWK 553
           DLDIG NNK+G TSY++AS+WG+KYFK NFKRLVS+KTKVDPSNFF NEQSIPS+ P WK
Sbjct: 481 DLDIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWK 540

BLAST of MC03g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 1087 bits (2812), Expect = 0.0
Identity = 538/540 (99.63%), Postives = 538/540 (99.63%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQ 313
           KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 314 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVL 373
           KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVL
Sbjct: 301 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVL 360

Query: 374 LDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESE 433
           LDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE E
Sbjct: 361 LDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEXE 420

Query: 434 IPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDI 493
           IPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDI
Sbjct: 421 IPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDI 480

Query: 494 GTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH 553
           GTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH
Sbjct: 481 GTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRGH 540

BLAST of MC03g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 444/541 (82.07%), Postives = 486/541 (89.83%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPI SLAYIFVVLSF  VASA NYEDFL CLSFH PN+SSISKI+YTP   SY  +LN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSIQNLRF  AKTPKPQ+IVTPL+ SH+QASIICAK+NG QIRTRSGGHDYEGLSYVS N
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVS-N 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPFVV+DLTNLRSIDVDVE+ VAWVQSGATIGELYYRIAEKSKTLGFPAGLC TVG+GGH
Sbjct: 121 VPFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYGVMLRKFGLAADNVIDAYFIDVNG L DRKSMGEDVFWAIRGGGGASFGI+LAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQ 313
           KIKLL VP +VTVFTITRNLEQNATNLI++WQYVA+KQDDRL+I++IL G  NS+N++N 
Sbjct: 241 KIKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGT-NSNNHRNG 300

Query: 314 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNV 373
           K+T+EA FNSLFLG+V++LLPIMQKTFPELGL KEDCIEMSWIE+VLYFA F +GQPLNV
Sbjct: 301 KRTIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNV 360

Query: 374 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISES 433
           LLDR   +KG+FKGKSDYVNEP+PKAG+EGIWELFNE+E +  QM LIPYGGKM+EISES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 434 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 493
           EIPFPHR GNLY+IHY++  G  E+  QR   WIRK YSYM PFVSKNPRAAYINYRDLD
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLD 480

Query: 494 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 553
           IGTNN++GNTSYDKASIWG+KYFK NFKRLVSVK KVDPSNFFRNEQSIPS   WWKK G
Sbjct: 481 IGTNNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPSR--WWKKGG 535

BLAST of MC03g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 882 bits (2280), Expect = 0.0
Identity = 431/541 (79.67%), Postives = 484/541 (89.46%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPITSLAYIF+ LS  WVASA N+EDFL CLS HSPN+S IS I+YTP D SY S+LN
Sbjct: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSIQN RF  AKTPKPQ+IVTPL+ SHIQAS+ICAK+NGFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSD- 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPFV++DLTN+RSIDVDVE+NVAWVQSGATIGELYYRIAEKSKTLGFPAGLC TVG+GGH
Sbjct: 121 VPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYG+MLRK+GLAADNVIDAYFIDVNG L DRKSMGEDVFWAIRG GGASFGIILAW
Sbjct: 181 FSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRG-GGASFGIILAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQ 313
           K+KL+ VPPVVTVF+ITRNL+QNATNL+H+WQYVA+KQDDRL+IR +L G+ NS N+QN 
Sbjct: 241 KVKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVL-GVTNSKNHQNG 300

Query: 314 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNV 373
           K+T++A+FNSLFLG+V ELLPIMQ TFPELGL KEDCIEMSWIE+VLYF+ F + QPLNV
Sbjct: 301 KRTVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNV 360

Query: 374 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISES 433
           L+DR+LA KG FKGKSDY+N+PIPKAG+EG+WEL   +EV+F +M L+PYGGKM+EISE+
Sbjct: 361 LIDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEA 420

Query: 434 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 493
           +IPFPHR  NLY IHY V   E  D+ QR ISWIRKLY YMTPFVSKNPRAAY NYRDLD
Sbjct: 421 DIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLD 480

Query: 494 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 553
           IGTNNKDGNT+Y+KASIWG KY+K NFKRLVS+KTKVDPSNFFRNEQSIPSL PWWK  G
Sbjct: 481 IGTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGG 537

BLAST of MC03g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1DE84 (berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111020245 PE=3 SV=1)

HSP 1 Score: 869 bits (2245), Expect = 1.09e-314
Identity = 426/543 (78.45%), Postives = 481/543 (88.58%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MKSPITSLAY FVV+SF+WVASASNYEDFL CLS HSPN+SSISKI+YTP D SY  I+N
Sbjct: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVS-D 133
           FS  NL   TAK+ KPQ IVTPLQVSHIQ SIICAK +GFQIRTRSGGHDYE LS +S D
Sbjct: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQXSIICAKKHGFQIRTRSGGHDYERLSSISYD 120

Query: 134 NVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGG 193
           ++PFVV+D+ N+RSIDV+VEKNVAWVQSGATIGELYYRIA+KSKTLGFPAGLC TVGVGG
Sbjct: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180

Query: 194 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILA 253
           HFSGGGYG+MLRKFGLAADNVIDAYF+DVNG L +RKS GEDVFWAIRGGGGASF II+A
Sbjct: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSXGEDVFWAIRGGGGASFXIIVA 240

Query: 254 WKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 313
           WKIKL+  PPVVTVF ITRNLEQNATNL+H+WQYVA+KQDD+LLI++ L GM NS+N+QN
Sbjct: 241 WKIKLVQXPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGM-NSNNHQN 300

Query: 314 QKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQG-QPLN 373
            K+T E SF+SLFLGQ +ELLPIMQ TFPELGLTKEDCIEMSWIE+VL+FAGF+G Q L+
Sbjct: 301 GKRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQXLD 360

Query: 374 VLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE 433
           VLLDR++A++   K K+DYVNEPIP  G++GIWELFNEEEV+FAQ+  IPYGGKM+EI E
Sbjct: 361 VLLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPE 420

Query: 434 SEIPFPHRVGNLYKIHYAVL-LGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 493
           SEIPFPHR GNLYKIHY+V+   E EDT Q+ I+WIRKL SYMTPFVSKNPRAAYINYRD
Sbjct: 421 SEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRD 480

Query: 494 LDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKK 553
           LDIG NNK+G TSY+KASIWG++YFK NFKRLVS+KTKVDP NFFR+EQSIPSL PWW K
Sbjct: 481 LDIGINNKNGYTSYEKASIWGLRYFKGNFKRLVSIKTKVDPYNFFRHEQSIPSLLPWWTK 540

BLAST of MC03g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1GTC1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE=3 SV=1)

HSP 1 Score: 866 bits (2237), Expect = 1.63e-313
Identity = 427/544 (78.49%), Postives = 479/544 (88.05%), Query Frame = 0

Query: 14  MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 73
           MK PIT   Y FVVLSF W ASA N+EDFL CLS HSPN+SSISKI+YTP D SY S+LN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 74  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 133
           FSI NLRFAT +TPKPQVIVTPLQV+ I+ASIICAK+ GFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSD- 120

Query: 134 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 193
           VPF+V+DL NLRSI+VD E NVAWVQSGATIGELYYRIA+KSKTLGFPAG+C TVGVGGH
Sbjct: 121 VPFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGH 180

Query: 194 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 253
           FSGGGYG+MLRKFGLA DNVIDAYFIDVNGNL DRKSMGEDVFWA+RGGGGASFGI++AW
Sbjct: 181 FSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAW 240

Query: 254 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN- 313
           KIKLL VPP+VTVFT+TRNLEQNATNLIH+WQY+ASKQDDRL IR+I+ G +NS+N Q+ 
Sbjct: 241 KIKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRG-VNSTNPQHG 300

Query: 314 QKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLN 373
            ++TLEA+FNS+FLG+ +ELLPIMQ TFPELGLTKEDCIEMSWIE++LYFAGF  GQPLN
Sbjct: 301 SQRTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLN 360

Query: 374 VLLDRS-LAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEIS 433
           VLLDR+ L  K  FK KSDYVNEPIPKA +EG WELFNEEE + A+M+L PYGG M EIS
Sbjct: 361 VLLDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEIS 420

Query: 434 ESEIPFPHRVGNLYKIHYAVLLGE-GEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYR 493
           ESE PFPHR GNLYKI + V   E GED A+R I+WIRKLYS++ P+VSKNPRAAYINYR
Sbjct: 421 ESETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYR 480

Query: 494 DLDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWK 553
           DLDIG NNK+G TSY++AS+WG+KYFK NFKRLVS+KTKVDPSNFF NEQSIPS+ P WK
Sbjct: 481 DLDIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWK 540

BLAST of MC03g_new0304 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 559.3 bits (1440), Expect = 3.5e-159
Identity = 281/537 (52.33%), Postives = 376/537 (70.02%), Query Frame = 0

Query: 20  SLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFS--------- 79
           +L  + ++L  V ++S++  +DF++CL     +NS +S     PI  S+FS         
Sbjct: 11  TLFLVTLLLISVPLSSSTLQQDFVKCL----VDNSDVS----FPITASFFSPDQNATLFK 70

Query: 80  -ILNFSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSY 139
             L  + QNLR+ T   PKP  I  PL  +H+QA+++CAK     +R RSGGHDYEGLS+
Sbjct: 71  EELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSF 130

Query: 140 VS-DNVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTV 199
           V+ D  PFV++DL+ LR +DVD++ N AW  +GATIGE+YYRI EKS+T GFPAGLCS++
Sbjct: 131 VAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSL 190

Query: 200 GVGGHFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFG 259
           G+GGH  GG YG M+RKFGL ADNV+DA  +D NG + DR +MGEDVFWAIRGGGG SFG
Sbjct: 191 GIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFG 250

Query: 260 IILAWKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSS 319
           +ILAWKIKL+PVP  VTVFT+T+ LEQ+ T ++++W+ +A K DD L IRVI++    ++
Sbjct: 251 VILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTT 310

Query: 320 NNQNQKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF--Q 379
              N  +T+  S+ + FLG  + LL +MQK+FPELGLTK+DC EMSWI++V+Y AGF   
Sbjct: 311 KPGN--RTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNS 370

Query: 380 GQPLNVLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKM 439
             P  +L  +SL +K  FK KSD+V EPIP  G+EG+WE F EE+      +  PYGG M
Sbjct: 371 AAPEALLAGKSL-FKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMM 430

Query: 440 SEISESEIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYI 499
           S ISESEIPFPHR G L+KI +     +G+ + +R + WIR++YSYM  +VSKNPR AY+
Sbjct: 431 SRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYV 490

Query: 500 NYRDLDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPS 544
           NYRDLD+GTN  +G T    A  WG KY+K NF+RLV +K + DP NFFR+EQS+P+
Sbjct: 491 NYRDLDLGTN--EGETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of MC03g_new0304 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 558.5 bits (1438), Expect = 5.9e-159
Identity = 273/515 (53.01%), Postives = 368/515 (71.46%), Query Frame = 0

Query: 34  ASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILNFSIQNLRFATAKTPKPQVIV 93
           A  +N+E+FL+CLS     N   S+I++T  D SYFSILN SIQN RF   +TPKP  I+
Sbjct: 29  AQRTNHENFLKCLSHRI--NEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 94  TPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVPFVVIDLTNLRSIDVDVEK 153
           TP+Q + +Q++I CA+ +G  IRTRSGGHDYEGLSY++ + PFVVIDL NLRSI +DV+ 
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 154 NVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYGVMLRKFGLAADNV 213
              WVQSGATIGELYY I + SK+L FPAGL  TVG+GG F GGGYG ++RK+GL+ADNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 214 IDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPVPPVVTVFTITRNL 273
           IDA+ +D NG+  DR+ MGED FWAIRGGGG+SF ++L+WKI+LL VP VVTVF + +  
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 274 EQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQKKT-LEASFNSLFLGQVDEL 333
           E+ A ++I++WQY+A K  + L IR +L           QK+T + ASF  L+LG V +L
Sbjct: 269 EKEAVSIINKWQYIADKVPNDLFIRAML-----------QKETEVYASFPGLYLGPVSDL 328

Query: 334 LPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVLLDRSLAWKGTFKGKSDYVN 393
           L +M+  FPELGL   +C EMSWIE+VL+F   +G+ + +L  R    + +FKGK D++ 
Sbjct: 329 LALMKDKFPELGLEIGNCREMSWIESVLWF--IKGESMEILAKRKRTSR-SFKGKDDFIE 388

Query: 394 EPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESEIPFPHRVGNLYKIHYAVLL 453
           EPIPK  I+ +W  F   E + A+++L P+GGKMSEI+++EIPFPHR GNLY+I Y    
Sbjct: 389 EPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYW 448

Query: 454 GEGED----TAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTN-NKDGNTSYDKA 513
            E ED      ++ + W+  +Y +MTP+VSK+PR AY+N+RD+D+G     +  T Y++A
Sbjct: 449 SEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEA 508

Query: 514 SIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIP 543
            +WG+KYFK+NF RLV VKT VDP +FF +EQSIP
Sbjct: 509 KVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0304 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 555.4 bits (1430), Expect = 5.0e-158
Identity = 268/532 (50.38%), Postives = 369/532 (69.36%), Query Frame = 0

Query: 22  AYIFVVLSF------VWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILNFS 81
           +++F +L F      +  A  SN+EDFL+CLS+   +N+   K+++T  D S+FSIL+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 82  IQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVP 141
           IQN RF+ ++TPKP  I+TP++ S +Q  I CA+ +G  +RTRS GH YEGLSY++ N P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 142 FVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFS 201
           F VIDL NLRSI +DV+    WVQ+GAT GELYY I + +K+L FPAG+  TVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 202 GGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKI 261
           GGGYG +LRK+GLAADN+IDA  +D +G + DR++MGED FWAIRGGGG+SFG+IL+WK+
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 262 KLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQKK 321
           KL+ VP  +TVF + +  ++ A  +I +WQY A K  D L IR  L         ++ K 
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL--------ERSNKN 304

Query: 322 TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNVLL 381
            + A F  L++G V+ LL +M++ FPELGL KE C EMSWIE+VL+FA F +G+ L VL 
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 364

Query: 382 DRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESEI 441
           +R      +FKGK D+V EPIP+A I+ IW      E +  +++L P+GGKMSE++E E 
Sbjct: 365 NRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424

Query: 442 PFPHRVGNLYKIHYAVLLGEGED----TAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 501
           PFPHR GNLY+I Y     E ED       + + W+  +Y +MTP+VSK+PR AY+N++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 502 LDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIP 543
           +D+G       T Y++   WG+KYFK+NF+RLV VKT+VDP++FF +EQSIP
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MC03g_new0304 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 555.1 bits (1429), Expect = 6.5e-158
Identity = 271/509 (53.24%), Postives = 360/509 (70.73%), Query Frame = 0

Query: 34  ASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILNFSIQNLRFATAKTPKPQVIV 93
           A+ SN   FL+CLS    +++ +SK+++TP D S+ S+L  SIQN RF+    PKP +I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 94  TPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDNVPFVVIDLTNLRSIDVDVEK 153
           TP+Q S +Q+++ CA+  G  IRTRSGGHDYEGLSYV+ + PFV++DL NLRSI VDV+ 
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT-HKPFVILDLRNLRSITVDVDN 148

Query: 154 NVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYGVMLRKFGLAADNV 213
              WVQ+GATIGELYY I +K++TL FPAG+C TVGVGGHFSGGGYG +LRK GLAAD+V
Sbjct: 149 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 208

Query: 214 IDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPVPPVVTVFTITRNL 273
           IDA  +D  G + +R+ MGED FWAIRGGGG+SF ++L+WKI L+ VP  VTVF +T+  
Sbjct: 209 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 268

Query: 274 EQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNQKKTLEASFNSLFLGQVDELL 333
           EQ+A  +IH+WQ+VA K  D L IRV+L         Q  K  + ASF  L+LG V  LL
Sbjct: 269 EQSALKIIHRWQFVADKVSDDLFIRVML---------QRYKNMVRASFPGLYLGSVKNLL 328

Query: 334 PIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNVLLDRSLAWKGTFKGKSDYVNE 393
            ++ K FPELGL ++DC EMSWIE+V++FA    +P+NVL  R+ A    FK KSD+V E
Sbjct: 329 KMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPINVLTKRTRA-SLAFKAKSDFVQE 388

Query: 394 PIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISESEIPFPHRVGNLYKIHYAVLLG 453
           P+PK  I  +W    E E + AQ++  P+GGKMSEI++ E PFPHR GN+Y+I Y  L  
Sbjct: 389 PMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQY--LNY 448

Query: 454 EGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTNNKDGNTSYDKASIWGMK 513
              D  ++ + W+ ++Y  M+ FV+K+PR AYIN RDLD+G       + Y++   WG+K
Sbjct: 449 WRGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVK 508

Query: 514 YFKSNFKRLVSVKTKVDPSNFFRNEQSIP 543
           YFK+NF+RLV VKT VDPS+FF +EQSIP
Sbjct: 509 YFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MC03g_new0304 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 552.4 bits (1422), Expect = 4.2e-157
Identity = 277/536 (51.68%), Postives = 372/536 (69.40%), Query Frame = 0

Query: 17  PITSLAYIFVVLSFVWVAS--ASNYEDFLRCLSFHSPNNSSISKIVYTPI-DISYFS-IL 76
           P+ S+  ++  L  +   S  AS  + F+ C+  ++     + K  + P  ++S FS +L
Sbjct: 8   PLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVL 67

Query: 77  NFSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSD 136
             + QNLRF     PKP  I +P+  SH+QASIIC+K     +R RSGGHDYEGLSYVS 
Sbjct: 68  ESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 127

Query: 137 -NVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVG 196
            + PF+++DL+ +R ++++++ N AWVQSGAT+GELYYRIAEKSK  GFPAGLCS++G+G
Sbjct: 128 IDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIG 187

Query: 197 GHFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIIL 256
           GH +GG YG M+RK+GL ADNV+DA  +D NG L DR +MGED FWAIRGG G SFGIIL
Sbjct: 188 GHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIIL 247

Query: 257 AWKIKLLPVPPVVTVFTITRNLEQNATN-LIHQWQYVASKQDDRLLIRVILNGMINSSNN 316
           AWKIKL+PVP  VTVFT+T+ L+Q+  N +I +WQ VA K  + L IRV+ N      N 
Sbjct: 248 AWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGN- 307

Query: 317 QNQKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQ-P 376
               KT+  S+N+LFLG    L+ +M+K+FPELGLT +DCIEMSW+E++ Y +GF    P
Sbjct: 308 ----KTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTP 367

Query: 377 LNVLLD-RSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSE 436
            NVLL  +S   K +FK KSD+V  PIP++G++GI++   +E++    M+  PYGG M++
Sbjct: 368 TNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAK 427

Query: 437 ISESEIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINY 496
           I ES+IPFPHR G L+K+ Y     + +    R I+WIR LYSYMTP+VS NPR AY+NY
Sbjct: 428 IPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNY 487

Query: 497 RDLDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSL 545
           RDLD+G N KD  T   +A +WG  YFK+NF RL+ +K KVDP NFFR+EQSIP +
Sbjct: 488 RDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536