MC03g_new0300 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0300 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.4e-156
Identity = 269/540 (49.81%), Postives = 369/540 (68.33%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLSAWVAS-----ACNHEDFLDCLSIHSPNSSPISNIIYTPTD--PS 60
           +  P+   + + ++ S +  +     A   + F++C+  ++    P+    + PT     
Sbjct: 3   ISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSM 62

Query: 61  YSSVLNFSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGL 120
           +S VL  + QN RFL    PKP  I +P+  SH+QAS+IC+K     +R RSGGHDYEGL
Sbjct: 63  FSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGL 122

Query: 121 SYVS--DVPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCP 180
           SYVS  D PF+L+DL+ +R ++++++ N AWVQSGAT+GELYYRIAEKSK  GFPAGLC 
Sbjct: 123 SYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCS 182

Query: 181 TVGIGGHFSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGG-GAS 240
           ++GIGGH +GG YG M+RKYGL ADNV+DA  +D NG+L DR +MGED FWAIRGG G S
Sbjct: 183 SLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGS 242

Query: 241 FGIILAWKVKLVLVPPVVTVFSITRNLKQNATN-LVHRWQYVANKQDDRLIIRAVLGVTN 300
           FGIILAWK+KLV VP  VTVF++T+ L+Q+  N ++ +WQ VA+K  + L IR +  V  
Sbjct: 243 FGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAG 302

Query: 301 SKNHQNGKRTVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFP 360
           +     G +TV  ++N+LFLG  G L+ +M+ +FPELGL  +DCIEMSW+ES+ Y S FP
Sbjct: 303 T----GGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFP 362

Query: 361 REQPLNVLID-RNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGG 420
              P NVL+  ++   K  FK KSD++  PIP++GL+G+++ L+ +++  +  +  PYGG
Sbjct: 363 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWN--PYGG 422

Query: 421 KMNEISEADIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAA 480
            M +I E+ IPFPHR   L+ + YV SW +      RHI+WIR LY YMTP+VS NPR A
Sbjct: 423 MMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREA 482

Query: 481 YFNYRDLDIGTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSL 529
           Y NYRDLD+G N KD  T  ++A +WGA Y+K+NF RL+ IK KVDP NFFR+EQSIP +
Sbjct: 483 YVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of MC03g_new0300 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 7.6e-156
Identity = 267/533 (50.09%), Postives = 358/533 (67.17%), Query Frame = 0

Query: 5   ITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNFSIQN 64
           +  + + F+     ++ A   E+FL C S H PN+     ++YT  D  Y S+LN +IQN
Sbjct: 11  VCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQN 70

Query: 65  QRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVPFVLV 124
            RF+   TPKP +IVTP   SHIQA+++C+K  G QIRTRSGGHD EG+SY+S VPFV+V
Sbjct: 71  LRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVV 130

Query: 125 DLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGY 184
           DL N+ SI +DV    AWV++GAT+GE+YY I EK++ L FP G CPTVG+GGHFSGGGY
Sbjct: 131 DLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGY 190

Query: 185 GLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGA-SFGIILAWKVKLVL 244
           G ++R YGLAADN+IDA+ ++V+G++ DRKSMGED+FWAIRGGG  +FGII AWK+KLV 
Sbjct: 191 GALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVA 250

Query: 245 VPPVVTVFSITRNLK-QNATNLVHRWQYVANKQDDRLIIRAVLGVTN-SKNHQNGKRTVK 304
           VP   T+FS+ +N++      L ++WQ +A K D  L++       N + NH   K TV 
Sbjct: 251 VPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVH 310

Query: 305 ANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLN----VL 364
             F+S+F G V  L+ +M  +FPELG+KK DC E SWI++ +++S        N    +L
Sbjct: 311 GYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEIL 370

Query: 365 IDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEAD 424
           +DR+   K  F  K DY+ +PIP+  +  + E L  ++V      L PYGG M EISE+ 
Sbjct: 371 LDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESA 430

Query: 425 IPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDI 484
           IPFPHRA  +Y + Y  SW +  D+E +HI+W+R +Y + TP+VS+NPR AY NYRDLD+
Sbjct: 431 IPFPHRAGIMYELWYTASWEKQEDNE-KHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDL 490

Query: 485 GTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFP 531
           G  N     NY +A IWG KY+  NF RLV +KTKVDP+NFFRNEQSIP L P
Sbjct: 491 GKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of MC03g_new0300 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 8.4e-155
Identity = 262/508 (51.57%), Postives = 360/508 (70.87%), Query Frame = 0

Query: 20  ASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNFSIQNQRFLGAKTPKPQIIV 79
           A+  N   FL CLS+   +S+ +S +I+TP D S+SSVL  SIQNQRF     PKP +I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 80  TPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVPFVLVDLTNIRSIDVDVEQN 139
           TP++ S +Q++V CA+  G  IRTRSGGHDYEGLSYV+  PFV++DL N+RSI VDV+  
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 140 VAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGLMLRKYGLAADNVI 199
             WVQ+GATIGELYY I +K++TL FPAG+CPTVG+GGHFSGGGYG +LRK+GLAAD+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 200 DAYFIDVNGELHDRKSMGEDVFWAIR-GGGASFGIILAWKVKLVLVPPVVTVFSITRNLK 259
           DA  +D  G + +R+ MGED FWAIR GGG+SF ++L+WK+ L+ VP  VTVF++T+  +
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 260 QNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTVKANFNSLFLGKVGELLPI 319
           Q+A  ++HRWQ+VA+K  D L IR +L        Q  K  V+A+F  L+LG V  LL +
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMVRASFPGLYLGSVKNLLKM 328

Query: 320 MQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDRNLALKGIFKGKSDYINQP 379
           +   FPELGL+++DC EMSWIESV++F++   E+P+NVL  R  A    FK KSD++ +P
Sbjct: 329 VNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEEPINVLTKRTRASLA-FKAKSDFVQEP 388

Query: 380 IPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPFPHRAENLYMIHYVVSWTE 439
           +PK  +  LW  L   E E  ++   P+GGKM+EI++ + PFPHR  N+Y I Y+  W  
Sbjct: 389 MPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWR- 448

Query: 440 VGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTNNKDGNTNYEKASIWGAKY 499
            GD +++++ W+ ++Y  M+ FV+K+PR AY N RDLD+G       + YE+   WG KY
Sbjct: 449 -GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKY 508

Query: 500 YKDNFKRLVSIKTKVDPSNFFRNEQSIP 527
           +K+NF+RLV +KT VDPS+FF +EQSIP
Sbjct: 509 FKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MC03g_new0300 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.1e-154
Identity = 264/533 (49.53%), Postives = 357/533 (66.98%), Query Frame = 0

Query: 5   ITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNFSIQN 64
           +  + + F+  +  ++ A   E+FL C S + PN+      IYT  D  Y SVLN +IQN
Sbjct: 11  VCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQN 70

Query: 65  QRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVPFVLV 124
            RF    TPKP +IVTP   SHIQAS++C+K  G QIRTRSGGHD EGLSY+S VPF +V
Sbjct: 71  LRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIV 130

Query: 125 DLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGY 184
           DL N+ ++ VD+    AWV++GAT+GE+YY I E ++   FP G CPTVG+GGHFSGGGY
Sbjct: 131 DLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGY 190

Query: 185 GLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGA-SFGIILAWKVKLVL 244
           G ++R YGLAADN+IDA+ ++V+G++ DRKSMGED+FWAIRGGG  +FGII AWK+KLV+
Sbjct: 191 GALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVV 250

Query: 245 VPPVVTVFSITRNLK-QNATNLVHRWQYVANKQDDRLIIRAVLGVTN-SKNHQNGKRTVK 304
           VP   T+FS+ +N++      L ++WQ +A K D  L++       N + NH   K TV 
Sbjct: 251 VPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVH 310

Query: 305 ANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLN----VL 364
             F+S+FLG V  L+ +M  +FPELG+KK DC E+SWI++ +++S        N    +L
Sbjct: 311 GYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEIL 370

Query: 365 IDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEAD 424
           +DR+   K  F  K DY+ + IP+  +  + E L  +EV      L PYGG M+EISE+ 
Sbjct: 371 LDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESA 430

Query: 425 IPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDI 484
           IPFPHRA  +Y + Y  +W +  D+E +HI+W+R +Y + TP+VS+NPR AY NYRDLD+
Sbjct: 431 IPFPHRAGIMYELWYTATWEKQEDNE-KHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDL 490

Query: 485 GTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFP 531
           G  N +   NY +A IWG KY+  NF RLV +KTK DP+NFFRNEQSIP L P
Sbjct: 491 GKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of MC03g_new0300 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.7e-153
Identity = 259/531 (48.78%), Postives = 359/531 (67.61%), Query Frame = 0

Query: 5   ITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNFSIQN 64
           +  + + F+  +  ++ A   E+FL CLS + P +   + ++YT  D  Y S+LN ++QN
Sbjct: 11  VCKIIFFFLSFNIQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQN 70

Query: 65  QRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVPFVLV 124
            RF    TPKP +I TPL  SHIQ +++C+K  G QIRTRSGGHD EG+SY+S VPFV+V
Sbjct: 71  LRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIV 130

Query: 125 DLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGY 184
           DL N+ S+ +DV    AWV+SGAT+GE+YY I E ++ L FPAG CPTVG GGHFSGGGY
Sbjct: 131 DLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGY 190

Query: 185 GLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGA-SFGIILAWKVKLVL 244
           G ++R YGLAADN+IDA+ ++V+G++ DRKSMGED+FWAIRGGG  +FGII AWK++LV 
Sbjct: 191 GALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVA 250

Query: 245 VPPVVTVFSITRNLK-QNATNLVHRWQYVANKQDDRLIIRAVLGVTN-SKNHQNGKRTVK 304
           VP + T+FS+ +N++      LV++WQ +A   +  L++       N + N    K T+ 
Sbjct: 251 VPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIH 310

Query: 305 ANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLN----VL 364
           + F+S+F G V  L+ +M  +FPELG+KK DC ++SWI++++++S        N    +L
Sbjct: 311 SYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEIL 370

Query: 365 IDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEAD 424
           +DR+   K  F  K DY+ +PIP+  +  + E L  ++V        PYGG M+EISE+ 
Sbjct: 371 LDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESA 430

Query: 425 IPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDI 484
           IPFPHRA   Y I Y+ SW +  D+E +HI+WIR +Y + TP+VS+NPR AY NYRDLD+
Sbjct: 431 IPFPHRAGITYEIWYIASWEKQEDNE-KHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDL 490

Query: 485 GTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSL 529
           G  N +   NY +A IWG KY+  NF RLV +KTKVDP NFFRNEQSIP L
Sbjct: 491 GKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of MC03g_new0300 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 1105 bits (2859), Expect = 0.0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNF 60
           MKSPITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNF
Sbjct: 1   MKSPITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNF 60

Query: 61  SIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVP 120
           SIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVP
Sbjct: 61  SIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVP 120

Query: 121 FVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFS 180
           FVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFS
Sbjct: 121 FVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFS 180

Query: 181 GGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVK 240
           GGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVK
Sbjct: 181 GGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVK 240

Query: 241 LVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTV 300
           LVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTV
Sbjct: 241 LVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTV 300

Query: 301 KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDR 360
           KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDR
Sbjct: 301 KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDR 360

Query: 361 NLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPF 420
           NLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPF
Sbjct: 361 NLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPF 420

Query: 421 PHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTN 480
           PHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTN
Sbjct: 421 PHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTN 480

Query: 481 NKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHETF 540
           NKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHETF
Sbjct: 481 NKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHETF 540

BLAST of MC03g_new0300 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 438/542 (80.81%), Postives = 482/542 (88.93%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPI SLAYIF+ LS A VASACN+EDFL CLS H PNSS IS IIYTPT PSYS VLN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQN RFLGAKTPKPQIIVTPLEASH+QAS+ICAKNNG QIRTRSGGHDYEGLSYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180
           PFV+VDLTN+RSIDVDVEQ VAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILAWK 240
           SGGGYG+MLRK+GLAADNVIDAYFIDVNG+LHDRKSMGEDVFWAIRGGG ASFGI+LAWK
Sbjct: 181 SGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 VKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR 300
           +KL+ VP +VTVF+ITRNL+QNATNL++RWQYVANKQDDRLII+ +L  TNS NH+NGKR
Sbjct: 241 IKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR 300

Query: 301 TVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLI 360
           T++A FNSLFLGKV +LLPIMQ TFPELGL KEDCIEMSWIESVLYF++FPR QPLNVL+
Sbjct: 301 TIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNVLL 360

Query: 361 DRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADI 420
           DR    KG FKGKSDY+N+P+PKAGLEG+WEL    E + V+M L+PYGGKMNEISE++I
Sbjct: 361 DRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESEI 420

Query: 421 PFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIG 480
           PFPHRA NLY IHY +     GD EQR+  WIRK Y YM PFVSKNPRAAY NYRDLDIG
Sbjct: 421 PFPHRAGNLYQIHYSIYCG--GDEEQRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIG 480

Query: 481 TNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHE 540
           TNN++GNT+Y+KASIWG KY+KDNFKRLVS+K KVDPSNFFRNEQSIPS   WWK GGHE
Sbjct: 481 TNNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPSR--WWKKGGHE 538

BLAST of MC03g_new0300 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 879 bits (2272), Expect = 0.0
Identity = 431/541 (79.67%), Postives = 483/541 (89.28%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPITSLAYIF+ LS  WVASA N+EDFL CLS HSPN+S IS I+YTP D SY S+LN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSD- 120
           FSIQN RF  AKTPKPQ+IVTPL+ SHIQAS+ICAK+NGFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180
           VPFV++DLTN+RSIDVDVE+NVAWVQSGATIGELYYRIAEKSKTLGFPAGLC TVG+GGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 181 FSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILAW 240
           FSGGGYG+MLRK+GLAADNVIDAYFIDVNG L DRKSMGEDVFWAIRGGG ASFGIILAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 241 KVKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVL-GVTNSKNHQNG 300
           K+KL+ VPPVVTVF+ITRNL+QNATNL+H+WQYVA+KQDDRL+IR +L G+ NS N+QN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 301 KRTVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNV 360
           K+T++A+FNSLFLG+V ELLPIMQ TFPELGL KEDCIEMSWIE+VLYF+ F + QPLNV
Sbjct: 301 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNV 360

Query: 361 LIDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEA 420
           L+DR+LA KG FKGKSDY+N+PIPKAG+EG+WEL   +EV+F +M L+PYGGKM+EISE 
Sbjct: 361 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEX 420

Query: 421 DIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLD 480
           +IPFPHR  NLY IHY V   E  D+ QR ISWIRKLY YMTPFVSKNPRAAY NYRDLD
Sbjct: 421 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 480

Query: 481 IGTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGG 537
           IGTNNKDGNT+Y+KASIWG KY+K NFKRLVS+KTKVDPSNFFRNEQSIPSL PWWK  G
Sbjct: 481 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 540

BLAST of MC03g_new0300 vs. NCBI nr
Match: XP_022152426.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 854 bits (2206), Expect = 1.10e-308
Identity = 411/542 (75.83%), Postives = 473/542 (87.27%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFL-SAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPITSLAYIF+    +WVASA N E+F+ CLS+HS N+S I NIIYT TDPSYSS+LN
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           +SI N+RF+GAKTPKPQ+IVTPL+ SHIQAS+ICA+ NGFQIRTRSGGHDYEG+SYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180
           PFVLVD  N+RSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHF
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240
           SGGGYG+MLRK+GLA DNVIDAY +DVNGELHDRKSMGEDVFWAIRGGGASFGIIL+WK+
Sbjct: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240

Query: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR- 300
           KLV VP VVTVF+I RNL++NAT+LVHRWQYVANKQDDRL+IR +L    S NHQNGKR 
Sbjct: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300

Query: 301 TVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDF-PREQPLNVL 360
           +++A+F+SLFLGKV ELLPIMQ TFPELG+ KEDC EMSWIESVLYF+DF P+ QPL+VL
Sbjct: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360

Query: 361 IDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEAD 420
           IDR+ A +G+FKGKSDY+++PIPK GL GLWELL   EV+F RMHL+PYGGKMNEISE++
Sbjct: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420

Query: 421 IPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDI 480
            P+PHRA NLY IHYVVSW    D  Q+HISWIRKLY YMTPFVSKNPR AY NYRDLD+
Sbjct: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480

Query: 481 GTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGH 539
           G NN +  T+YEKASIWG KYYKDNF+RLVSIKTKVDP++FF N+QSIPSL    K   H
Sbjct: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540

BLAST of MC03g_new0300 vs. NCBI nr
Match: XP_022152442.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 850 bits (2197), Expect = 2.23e-307
Identity = 409/540 (75.74%), Postives = 469/540 (86.85%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPI+SLAYIF+ LS +WVASA NHEDFL CLS H PN S IS IIYTPTDP Y SVLN
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQN RFLG KT KPQ+IVTPL+ASHIQAS+ICAK NGFQIR RSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180
           PFV+VD+ N+RSIDVDVE+ VAWV+SGATIGELYYRIAEKSKTL FPAGL  TVG+GGHF
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHF 180

Query: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240
           SGGGYG ++RK+GLAADNVIDAYFIDVNG+LHDR+SMGED+FWAIRGGGASFGIIL+WKV
Sbjct: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240

Query: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRT 300
           KLV VPP+VT+F ITRNL+QNAT+LVH+WQYVANKQDDRLIIR +L  TN  NHQNGKR 
Sbjct: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300

Query: 301 V-KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLI 360
           V +A F   FLGKV ELLPIMQNTFPELGL+KED IEMSWIES++YF+  P+ Q  + L+
Sbjct: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQA-SALL 360

Query: 361 DRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADI 420
           DR+LALKG +KGK DY+N+PIPKAGLEG+WELL   +++F ++ L+PYGG+M+EISE++I
Sbjct: 361 DRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEI 420

Query: 421 PFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIG 480
           PFPHRA N+Y IHY V+++  GD+EQRHI WIRK Y Y+TPFVSKNPRA Y NYRDLDIG
Sbjct: 421 PFPHRAGNIYKIHYTVAYS--GDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIG 480

Query: 481 TNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHE 538
            NNK+G T+YEKAS+WG KY+K NFKRLV +K KVDP+NFFRNEQSIPS   WWK  GHE
Sbjct: 481 MNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHE 537

BLAST of MC03g_new0300 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 1105 bits (2859), Expect = 0.0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNF 60
           MKSPITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNF
Sbjct: 1   MKSPITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNF 60

Query: 61  SIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVP 120
           SIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVP
Sbjct: 61  SIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVP 120

Query: 121 FVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFS 180
           FVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFS
Sbjct: 121 FVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFS 180

Query: 181 GGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVK 240
           GGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVK
Sbjct: 181 GGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKVK 240

Query: 241 LVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTV 300
           LVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTV
Sbjct: 241 LVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTV 300

Query: 301 KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDR 360
           KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDR
Sbjct: 301 KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDR 360

Query: 361 NLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPF 420
           NLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPF
Sbjct: 361 NLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPF 420

Query: 421 PHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTN 480
           PHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTN
Sbjct: 421 PHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTN 480

Query: 481 NKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHETF 540
           NKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHETF
Sbjct: 481 NKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHETF 540

BLAST of MC03g_new0300 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 438/542 (80.81%), Postives = 482/542 (88.93%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPI SLAYIF+ LS A VASACN+EDFL CLS H PNSS IS IIYTPT PSYS VLN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQN RFLGAKTPKPQIIVTPLEASH+QAS+ICAKNNG QIRTRSGGHDYEGLSYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180
           PFV+VDLTN+RSIDVDVEQ VAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILAWK 240
           SGGGYG+MLRK+GLAADNVIDAYFIDVNG+LHDRKSMGEDVFWAIRGGG ASFGI+LAWK
Sbjct: 181 SGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 VKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR 300
           +KL+ VP +VTVF+ITRNL+QNATNL++RWQYVANKQDDRLII+ +L  TNS NH+NGKR
Sbjct: 241 IKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR 300

Query: 301 TVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLI 360
           T++A FNSLFLGKV +LLPIMQ TFPELGL KEDCIEMSWIESVLYF++FPR QPLNVL+
Sbjct: 301 TIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNVLL 360

Query: 361 DRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADI 420
           DR    KG FKGKSDY+N+P+PKAGLEG+WEL    E + V+M L+PYGGKMNEISE++I
Sbjct: 361 DRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESEI 420

Query: 421 PFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIG 480
           PFPHRA NLY IHY +     GD EQR+  WIRK Y YM PFVSKNPRAAY NYRDLDIG
Sbjct: 421 PFPHRAGNLYQIHYSIYCG--GDEEQRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIG 480

Query: 481 TNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHE 540
           TNN++GNT+Y+KASIWG KY+KDNFKRLVS+K KVDPSNFFRNEQSIPS   WWK GGHE
Sbjct: 481 TNNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPSR--WWKKGGHE 538

BLAST of MC03g_new0300 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 879 bits (2272), Expect = 0.0
Identity = 431/541 (79.67%), Postives = 483/541 (89.28%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPITSLAYIF+ LS  WVASA N+EDFL CLS HSPN+S IS I+YTP D SY S+LN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSD- 120
           FSIQN RF  AKTPKPQ+IVTPL+ SHIQAS+ICAK+NGFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180
           VPFV++DLTN+RSIDVDVE+NVAWVQSGATIGELYYRIAEKSKTLGFPAGLC TVG+GGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 181 FSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILAW 240
           FSGGGYG+MLRK+GLAADNVIDAYFIDVNG L DRKSMGEDVFWAIRGGG ASFGIILAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 241 KVKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVL-GVTNSKNHQNG 300
           K+KL+ VPPVVTVF+ITRNL+QNATNL+H+WQYVA+KQDDRL+IR +L G+ NS N+QN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 301 KRTVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNV 360
           K+T++A+FNSLFLG+V ELLPIMQ TFPELGL KEDCIEMSWIE+VLYF+ F + QPLNV
Sbjct: 301 KKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGF-QGQPLNV 360

Query: 361 LIDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEA 420
           L+DR+LA KG FKGKSDY+N+PIPKAG+EG+WEL   +EV+F +M L+PYGGKM+EISE 
Sbjct: 361 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEX 420

Query: 421 DIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLD 480
           +IPFPHR  NLY IHY V   E  D+ QR ISWIRKLY YMTPFVSKNPRAAY NYRDLD
Sbjct: 421 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 480

Query: 481 IGTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGG 537
           IGTNNKDGNT+Y+KASIWG KY+K NFKRLVS+KTKVDPSNFFRNEQSIPSL PWWK  G
Sbjct: 481 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 540

BLAST of MC03g_new0300 vs. ExPASy TrEMBL
Match: A0A6J1DHP3 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 PE=3 SV=1)

HSP 1 Score: 854 bits (2206), Expect = 5.34e-309
Identity = 411/542 (75.83%), Postives = 473/542 (87.27%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFL-SAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPITSLAYIF+    +WVASA N E+F+ CLS+HS N+S I NIIYT TDPSYSS+LN
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           +SI N+RF+GAKTPKPQ+IVTPL+ SHIQAS+ICA+ NGFQIRTRSGGHDYEG+SYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180
           PFVLVD  N+RSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHF
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240
           SGGGYG+MLRK+GLA DNVIDAY +DVNGELHDRKSMGEDVFWAIRGGGASFGIIL+WK+
Sbjct: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240

Query: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR- 300
           KLV VP VVTVF+I RNL++NAT+LVHRWQYVANKQDDRL+IR +L    S NHQNGKR 
Sbjct: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300

Query: 301 TVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDF-PREQPLNVL 360
           +++A+F+SLFLGKV ELLPIMQ TFPELG+ KEDC EMSWIESVLYF+DF P+ QPL+VL
Sbjct: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360

Query: 361 IDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEAD 420
           IDR+ A +G+FKGKSDY+++PIPK GL GLWELL   EV+F RMHL+PYGGKMNEISE++
Sbjct: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420

Query: 421 IPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDI 480
            P+PHRA NLY IHYVVSW    D  Q+HISWIRKLY YMTPFVSKNPR AY NYRDLD+
Sbjct: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480

Query: 481 GTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGH 539
           G NN +  T+YEKASIWG KYYKDNF+RLVSIKTKVDP++FF N+QSIPSL    K   H
Sbjct: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540

BLAST of MC03g_new0300 vs. ExPASy TrEMBL
Match: A0A6J1DG99 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 PE=3 SV=1)

HSP 1 Score: 850 bits (2197), Expect = 1.08e-307
Identity = 409/540 (75.74%), Postives = 469/540 (86.85%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60
           MKSPI+SLAYIF+ LS +WVASA NHEDFL CLS H PN S IS IIYTPTDP Y SVLN
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQN RFLG KT KPQ+IVTPL+ASHIQAS+ICAK NGFQIR RSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180
           PFV+VD+ N+RSIDVDVE+ VAWV+SGATIGELYYRIAEKSKTL FPAGL  TVG+GGHF
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHF 180

Query: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240
           SGGGYG ++RK+GLAADNVIDAYFIDVNG+LHDR+SMGED+FWAIRGGGASFGIIL+WKV
Sbjct: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240

Query: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRT 300
           KLV VPP+VT+F ITRNL+QNAT+LVH+WQYVANKQDDRLIIR +L  TN  NHQNGKR 
Sbjct: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300

Query: 301 V-KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLI 360
           V +A F   FLGKV ELLPIMQNTFPELGL+KED IEMSWIES++YF+  P+ Q  + L+
Sbjct: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQA-SALL 360

Query: 361 DRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADI 420
           DR+LALKG +KGK DY+N+PIPKAGLEG+WELL   +++F ++ L+PYGG+M+EISE++I
Sbjct: 361 DRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEI 420

Query: 421 PFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIG 480
           PFPHRA N+Y IHY V+++  GD+EQRHI WIRK Y Y+TPFVSKNPRA Y NYRDLDIG
Sbjct: 421 PFPHRAGNIYKIHYTVAYS--GDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIG 480

Query: 481 TNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHE 538
            NNK+G T+YEKAS+WG KY+K NFKRLV +K KVDP+NFFRNEQSIPS   WWK  GHE
Sbjct: 481 MNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHE 537

BLAST of MC03g_new0300 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.4e-157
Identity = 269/540 (49.81%), Postives = 369/540 (68.33%), Query Frame = 0

Query: 1   MKSPITSLAYIFIFLSAWVAS-----ACNHEDFLDCLSIHSPNSSPISNIIYTPTD--PS 60
           +  P+   + + ++ S +  +     A   + F++C+  ++    P+    + PT     
Sbjct: 3   ISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSM 62

Query: 61  YSSVLNFSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGL 120
           +S VL  + QN RFL    PKP  I +P+  SH+QAS+IC+K     +R RSGGHDYEGL
Sbjct: 63  FSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGL 122

Query: 121 SYVS--DVPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCP 180
           SYVS  D PF+L+DL+ +R ++++++ N AWVQSGAT+GELYYRIAEKSK  GFPAGLC 
Sbjct: 123 SYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCS 182

Query: 181 TVGIGGHFSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGG-GAS 240
           ++GIGGH +GG YG M+RKYGL ADNV+DA  +D NG+L DR +MGED FWAIRGG G S
Sbjct: 183 SLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGS 242

Query: 241 FGIILAWKVKLVLVPPVVTVFSITRNLKQNATN-LVHRWQYVANKQDDRLIIRAVLGVTN 300
           FGIILAWK+KLV VP  VTVF++T+ L+Q+  N ++ +WQ VA+K  + L IR +  V  
Sbjct: 243 FGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAG 302

Query: 301 SKNHQNGKRTVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFP 360
           +     G +TV  ++N+LFLG  G L+ +M+ +FPELGL  +DCIEMSW+ES+ Y S FP
Sbjct: 303 T----GGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFP 362

Query: 361 REQPLNVLID-RNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGG 420
              P NVL+  ++   K  FK KSD++  PIP++GL+G+++ L+ +++  +  +  PYGG
Sbjct: 363 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWN--PYGG 422

Query: 421 KMNEISEADIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAA 480
            M +I E+ IPFPHR   L+ + YV SW +      RHI+WIR LY YMTP+VS NPR A
Sbjct: 423 MMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREA 482

Query: 481 YFNYRDLDIGTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSL 529
           Y NYRDLD+G N KD  T  ++A +WGA Y+K+NF RL+ IK KVDP NFFR+EQSIP +
Sbjct: 483 YVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of MC03g_new0300 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 548.5 bits (1412), Expect = 6.0e-156
Identity = 262/508 (51.57%), Postives = 360/508 (70.87%), Query Frame = 0

Query: 20  ASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNFSIQNQRFLGAKTPKPQIIV 79
           A+  N   FL CLS+   +S+ +S +I+TP D S+SSVL  SIQNQRF     PKP +I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 80  TPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDVPFVLVDLTNIRSIDVDVEQN 139
           TP++ S +Q++V CA+  G  IRTRSGGHDYEGLSYV+  PFV++DL N+RSI VDV+  
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 140 VAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGLMLRKYGLAADNVI 199
             WVQ+GATIGELYY I +K++TL FPAG+CPTVG+GGHFSGGGYG +LRK+GLAAD+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 200 DAYFIDVNGELHDRKSMGEDVFWAIR-GGGASFGIILAWKVKLVLVPPVVTVFSITRNLK 259
           DA  +D  G + +R+ MGED FWAIR GGG+SF ++L+WK+ L+ VP  VTVF++T+  +
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 260 QNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTVKANFNSLFLGKVGELLPI 319
           Q+A  ++HRWQ+VA+K  D L IR +L        Q  K  V+A+F  L+LG V  LL +
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMVRASFPGLYLGSVKNLLKM 328

Query: 320 MQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDRNLALKGIFKGKSDYINQP 379
           +   FPELGL+++DC EMSWIESV++F++   E+P+NVL  R  A    FK KSD++ +P
Sbjct: 329 VNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEEPINVLTKRTRASLA-FKAKSDFVQEP 388

Query: 380 IPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPFPHRAENLYMIHYVVSWTE 439
           +PK  +  LW  L   E E  ++   P+GGKM+EI++ + PFPHR  N+Y I Y+  W  
Sbjct: 389 MPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWR- 448

Query: 440 VGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTNNKDGNTNYEKASIWGAKY 499
            GD +++++ W+ ++Y  M+ FV+K+PR AY N RDLD+G       + YE+   WG KY
Sbjct: 449 -GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKY 508

Query: 500 YKDNFKRLVSIKTKVDPSNFFRNEQSIP 527
           +K+NF+RLV +KT VDPS+FF +EQSIP
Sbjct: 509 FKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MC03g_new0300 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 542.7 bits (1397), Expect = 3.3e-154
Identity = 264/531 (49.72%), Postives = 361/531 (67.98%), Query Frame = 0

Query: 2   KSPITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPS--YSSVLN 61
           K   T      + +S  ++S+   +DF+ CL  +S  S PI+   ++P   +  +   L 
Sbjct: 7   KRNATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELE 66

Query: 62  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVS-- 121
            + QN R+L    PKP  I  PL  +H+QA+V+CAK     +R RSGGHDYEGLS+V+  
Sbjct: 67  STAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAED 126

Query: 122 DVPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGG 181
           + PFV+VDL+ +R +DVD++ N AW  +GATIGE+YYRI EKS+T GFPAGLC ++GIGG
Sbjct: 127 ETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGG 186

Query: 182 HFSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIR-GGGASFGIILA 241
           H  GG YG M+RK+GL ADNV+DA  +D NG++ DR +MGEDVFWAIR GGG SFG+ILA
Sbjct: 187 HLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILA 246

Query: 242 WKVKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNG 301
           WK+KLV VP  VTVF++T+ L+Q+ T ++++W+ +A+K DD L IR ++    SK  + G
Sbjct: 247 WKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPA-SKTTKPG 306

Query: 302 KRTVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNV 361
            RT+  ++ + FLG    LL +MQ +FPELGL K+DC EMSWI+SV+Y + FP       
Sbjct: 307 NRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEA 366

Query: 362 LIDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEA 421
           L+      K  FK KSD++ +PIP  GLEGLWE  + ++      +  PYGG M+ ISE+
Sbjct: 367 LLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWN--PYGGMMSRISES 426

Query: 422 DIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLD 481
           +IPFPHR   L+ I ++ +W +   SE+RH+ WIR++Y YM  +VSKNPR AY NYRDLD
Sbjct: 427 EIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLD 486

Query: 482 IGTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPS 528
           +GTN  +G T+   A  WGAKYYK NF+RLV IK + DP NFFR+EQS+P+
Sbjct: 487 LGTN--EGETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of MC03g_new0300 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 540.0 bits (1390), Expect = 2.1e-153
Identity = 256/513 (49.90%), Postives = 354/513 (69.01%), Query Frame = 0

Query: 20  ASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLNFSIQNQRFLGAKTPKPQIIV 79
           A   NHEDFL CLS    +++    +I+T  D S+ S+L+ SIQN RF  ++TPKP  I+
Sbjct: 23  AHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSII 82

Query: 80  TPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVS-DVPFVLVDLTNIRSIDVDVEQ 139
           TP++AS +Q  + CA+ +G  +RTRS GH YEGLSY++ + PF ++DL N+RSI +DV+ 
Sbjct: 83  TPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDN 142

Query: 140 NVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGLMLRKYGLAADNV 199
              WVQ+GAT GELYY I + +K+L FPAG+ PTVG+GG FSGGGYG +LRKYGLAADN+
Sbjct: 143 RTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNI 202

Query: 200 IDAYFIDVNGELHDRKSMGEDVFWAIR-GGGASFGIILAWKVKLVLVPPVVTVFSITRNL 259
           IDA  +D +G + DR++MGED FWAIR GGG+SFG+IL+WKVKLV VP  +TVF + +  
Sbjct: 203 IDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTS 262

Query: 260 KQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRTVKANFNSLFLGKVGELLP 319
           K+ A  ++ +WQY A+K  D L IR  L        ++ K  V A F  L++G V  LL 
Sbjct: 263 KKEAVRIIKKWQYAADKVPDDLFIRTTL-------ERSNKNAVHALFTGLYIGPVNNLLA 322

Query: 320 IMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLIDRNLALKGIFKGKSDYINQ 379
           +M+  FPELGL+KE C EMSWIESVL+F+DFP+ + L VL +R       FKGK D++ +
Sbjct: 323 LMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRERTSLS-FKGKDDFVQE 382

Query: 380 PIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIPFPHRAENLYMIHYVVSWT 439
           PIP+A ++ +W  L   E    ++ L P+GGKM+E++E + PFPHR  NLY I YV  W 
Sbjct: 383 PIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWR 442

Query: 440 EVGDSEQ----RHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGTNNKDGNTNYEKASI 499
           E  D  +    +++ W+  +Y +MTP+VSK+PR AY N++D+D+G       T YE+   
Sbjct: 443 EEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKS 502

Query: 500 WGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIP 527
           WG KY+K+NF+RLV +KT+VDP++FF +EQSIP
Sbjct: 503 WGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MC03g_new0300 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.5e-151
Identity = 263/528 (49.81%), Postives = 357/528 (67.61%), Query Frame = 0

Query: 5   ITSLAYIFIFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTP--TDPSYSSVLNFSI 64
           + SL+YI +  S         +DF+ CL  +S  S P+ +  +T      S+   L  S 
Sbjct: 18  LLSLSYIPLSFST------IQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 77

Query: 65  QNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV--P 124
           QN R+L    PKP+ I  PL  +H+QA+V+CAK     +R RSGGHDYEGLSYVS++   
Sbjct: 78  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 137

Query: 125 FVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFS 184
           FV+VDL+ +R I VD+E N AWV +GA+IGE+YYRI EKSK  GFPAGLC ++GIGGH  
Sbjct: 138 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 197

Query: 185 GGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIR-GGGASFGIILAWKV 244
           GG YG M+RK+GL ADNV+DA  +D +G++ +R +MGEDVFWAIR GGG SFG+ILAWK+
Sbjct: 198 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 257

Query: 245 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRT 304
           KLV VP +VTVF++TR L+Q+ T L+++WQ VA+K D+ L IR ++  T SK  ++ +RT
Sbjct: 258 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPT-SKTPKSKERT 317

Query: 305 VKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLID 364
           +  ++   FLG    LL +MQ +FP+LGL K+DC+E SWI+SV+Y + FP   P   L+D
Sbjct: 318 ISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD 377

Query: 365 RNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADIP 424
                K  FK KSDY+ +PIP  GLEGLWE L+ ++      +  PYGG M +I E + P
Sbjct: 378 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWN--PYGGMMAKIPETETP 437

Query: 425 FPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIGT 484
           FPHR+  L+ I ++  W +   SE +H+ W+R++Y YM  +VSK+PR+AY NYRDLD+G 
Sbjct: 438 FPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGM 497

Query: 485 NNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPS 528
           N K  +     A  WG +Y+K NF+RLV IK K DP NFFR+EQSIP+
Sbjct: 498 NGKGSD-----AREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531