MC03g_new0295 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0295 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.4e-157
Identity = 264/510 (51.76%), Postives = 372/510 (72.94%), Query Frame = 0

Query: 21  ASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYSIMNERFMGAKTPKPQVIV 80
           A+  NQ +F+ CLSL   +++ +  +I+T  D S+SS+L  SI N+RF     PKP +I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 81  TPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRSIDIDVERK 140
           TP+Q S +Q+++ CAR+ G  IRTRSGGHDYEG+SYV+  PFV++D  NLRSI +DV+ +
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 141 VAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFGLAIDNVI 200
             WVQ+GAT+GELYY+I +K++TL +P GVCPTVGVGGHFSGGGYG +LRK GLA D+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 201 DAYLVDVNGELHDRKSMGEDVFWAIR-GGGASFGIILSWKIKLVSVPSVVTVFTIKRNLE 260
           DA +VD  G + +R+ MGED FWAIR GGG+SF ++LSWKI L++VPS VTVF + +  E
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 261 ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFLGKVEELLP 320
           ++A  ++HRWQ+VA+K  D L IR++L   K+          + ASF  L+LG V+ LL 
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRVMLQRYKN---------MVRASFPGLYLGSVKNLLK 328

Query: 321 IMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVLIDRSFAFRGVFKGKSDYVD 380
           ++ + FPELG+ ++DCTEMSWIESV++FA  E   +P++VL  R+ A    FK KSD+V 
Sbjct: 329 MVNKEFPELGLEEDDCTEMSWIESVIWFA--ELGEEPINVLTKRTRASL-AFKAKSDFVQ 388

Query: 381 EPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHRAGNLYNIHYVVSW 440
           EP+PKT +  LW  L + E + A++   P+GGKM+EI++ ETP+PHR GN+Y I Y+  W
Sbjct: 389 EPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW 448

Query: 441 GGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEYTSYEKASIWGL 500
            G  D+ +K++ W+ ++Y+ M+ FV+K+PRGAY+N RDLDLG+    + + YE+   WG+
Sbjct: 449 RG--DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGV 508

Query: 501 KYYKDNFERLVSIKTKVDPTDFFTNQQSIP 530
           KY+K+NFERLV +KT VDP+DFF ++QSIP
Sbjct: 509 KYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MC03g_new0295 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.5e-154
Identity = 266/516 (51.55%), Postives = 363/516 (70.35%), Query Frame = 0

Query: 21  ASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYSIMNERFMGAKTPKPQVIV 80
           A   N ENF+ CLS H +N      II+TS DPSY SILN SI N RF   +TPKP  I+
Sbjct: 29  AQRTNHENFLKCLS-HRINEDD-SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 81  TPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVS-DVPFVLVDFVNLRSIDIDVER 140
           TP+Q + +Q++I CAR +G  IRTRSGGHDYEG+SY++   PFV++D  NLRSI +DV+ 
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 141 KVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFGLAIDNV 200
           +  WVQSGAT+GELYY+I + SK+L +P G+ PTVG+GG F GGGYG ++RK+GL+ DNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 201 IDAYLVDVNGELHDRKSMGEDVFWAIR-GGGASFGIILSWKIKLVSVPSVVTVFTIKRNL 260
           IDA++VD NG   DR+ MGED FWAIR GGG+SF ++LSWKI+L+ VPSVVTVF + +  
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 261 EENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFLGKVEELL 320
           E+ A  ++++WQY+A+K  + L IR +L           K   + ASF  L+LG V +LL
Sbjct: 269 EKEAVSIINKWQYIADKVPNDLFIRAMLQ----------KETEVYASFPGLYLGPVSDLL 328

Query: 321 PIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVLIDRSFAFRGVFKGKSDYV 380
            +M+  FPELG+   +C EMSWIESVL+F     K + +++L  R    R  FKGK D++
Sbjct: 329 ALMKDKFPELGLEIGNCREMSWIESVLWFI----KGESMEILAKRKRTSRS-FKGKDDFI 388

Query: 381 DEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHRAGNLYNIHYVVS 440
           +EPIPKT +  LW      E + A++ L P+GGKM+EI+++E P+PHR GNLY I Y+  
Sbjct: 389 EEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAY 448

Query: 441 WGGDED----IAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGIN-NINEYTSYEK 500
           W  +ED      +K++ W+  +Y +MTP+VSK+PR AYVN+RD+DLG+   +N  T YE+
Sbjct: 449 WSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEE 508

Query: 501 ASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 530
           A +WG+KY+K+NF+RLV +KT VDP DFF ++QSIP
Sbjct: 509 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0295 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.6e-153
Identity = 267/525 (50.86%), Postives = 364/525 (69.33%), Query Frame = 0

Query: 12  FVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYSIMNERFMGA 71
           F +S+   ++ A  +ENF+ C S H  N  +   ++YT  D  Y SILN +I N RF+  
Sbjct: 17  FFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISD 76

Query: 72  KTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLR 131
            TPKP VIVTP   SHIQA+I+C++K G QIRTRSGGHD EG+SY+S VPFV+VD  N+ 
Sbjct: 77  TTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMH 136

Query: 132 SIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRK 191
           SI IDV  + AWV++GATLGE+YY I EK++ L +PGG CPTVGVGGHFSGGGYG ++R 
Sbjct: 137 SIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRN 196

Query: 192 FGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGA-SFGIILSWKIKLVSVPSVVT 251
           +GLA DN+IDA+LV+V+G++ DRKSMGED+FWAIRGGG  +FGII +WKIKLV+VPS  T
Sbjct: 197 YGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKST 256

Query: 252 VFTIKRNLE-ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSL 311
           +F++K+N+E      L ++WQ +A K D  LV+         +++    + ++   FSS+
Sbjct: 257 IFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSI 316

Query: 312 FLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFA---DFEPKRQPLDVLIDRSFA 371
           F G V+ L+ +M ++FPELG+ K DC E SWI++ ++++   +F       ++L+DRS  
Sbjct: 317 FHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAG 376

Query: 372 FRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHR 431
            +  F  K DYV +PIP+T ++ + E L +++V      L PYGG M EISES  P+PHR
Sbjct: 377 KKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHR 436

Query: 432 AGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNIN 491
           AG +Y + Y  SW   ED  +KHI+W+R +YN+ TP+VS+NPR AY+NYRDLDLG  N  
Sbjct: 437 AGIMYELWYTASWEKQED-NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHA 496

Query: 492 EYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSL 532
              +Y +A IWG KY+  NF RLV +KTKVDP +FF N+QSIP L
Sbjct: 497 SPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of MC03g_new0295 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.3e-152
Identity = 261/525 (49.71%), Postives = 365/525 (69.52%), Query Frame = 0

Query: 12  FVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYSIMNERFMGA 71
           F +S+   ++ A  QENF+ CLS +     +   ++YT  D  Y SILN ++ N RF   
Sbjct: 17  FFLSFNIQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSD 76

Query: 72  KTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLR 131
            TPKP VI TPL VSHIQ +I+C++K G QIRTRSGGHD EG+SY+S VPFV+VD  N+ 
Sbjct: 77  TTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMH 136

Query: 132 SIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRK 191
           S+ IDV  + AWV+SGATLGE+YY I E ++ L +P G CPTVG GGHFSGGGYG ++R 
Sbjct: 137 SVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRN 196

Query: 192 FGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGA-SFGIILSWKIKLVSVPSVVT 251
           +GLA DN+IDA+LV+V+G++ DRKSMGED+FWAIRGGG  +FGII +WKI+LV+VPS+ T
Sbjct: 197 YGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMST 256

Query: 252 VFTIKRNLE-ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSL 311
           +F++K+N+E      LV++WQ +A   +  L++         +++Q   + +I + FSS+
Sbjct: 257 IFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSI 316

Query: 312 FLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFA---DFEPKRQPLDVLIDRSFA 371
           F G V+ L+ +M ++FPELG+ K DC ++SWI+++++++   ++       ++L+DRS  
Sbjct: 317 FHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGG 376

Query: 372 FRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHR 431
            +  F  K DYV +PIP+T ++ + E L +++V        PYGG M+EISES  P+PHR
Sbjct: 377 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 436

Query: 432 AGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNIN 491
           AG  Y I Y+ SW   ED  +KHI+WIR +YN+ TP+VS+NPR AY+NYRDLDLG  N  
Sbjct: 437 AGITYEIWYIASWEKQED-NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFE 496

Query: 492 EYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSL 532
              +Y +A IWG KY+  NF RLV +KTKVDP +FF N+QSIP L
Sbjct: 497 SPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of MC03g_new0295 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 2.3e-152
Identity = 260/525 (49.52%), Postives = 366/525 (69.71%), Query Frame = 0

Query: 12  FVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYSIMNERFMGA 71
           F +S+   ++ A  QENF+ C S +     +   ++YT  D  Y SILN +I N RF   
Sbjct: 17  FFLSFNIQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSD 76

Query: 72  KTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLR 131
            TPKP VI+TPL VSHIQ +I+C++K G QIRTRSGGHD EG+SY+S VPFV+VD  N+ 
Sbjct: 77  TTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMH 136

Query: 132 SIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRK 191
           S+ IDV  + AWV++GATLGE+YY I E ++ L +P G CPTVG GGHFSGGGYG ++R 
Sbjct: 137 SVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRN 196

Query: 192 FGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGA-SFGIILSWKIKLVSVPSVVT 251
           +GLA DN+IDA+LV+V+G++ DRKSMGED+FWAIRGGG  +FGII +WKI+LV+VPS+ T
Sbjct: 197 YGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMST 256

Query: 252 VFTIKRNLE-ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSL 311
           +F++K+N+E      LV++WQ +A   +  L++         +++Q   + +I + FSS+
Sbjct: 257 IFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSI 316

Query: 312 FLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFA---DFEPKRQPLDVLIDRSFA 371
           F G V+ L+ +M ++FPELG+ K DC ++SWI+++++++   ++       ++L+DRS  
Sbjct: 317 FHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGG 376

Query: 372 FRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHR 431
            +  F  K DYV +PIP+T ++ + E L +++V        PYGG M+EISES  P+PHR
Sbjct: 377 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 436

Query: 432 AGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNIN 491
           AG +Y I Y+ SW   ED  +KHI+WIR +YN+ TP+VS+NPR AY+NYRDLDLG  N  
Sbjct: 437 AGIMYEIWYIASWEKQED-NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFE 496

Query: 492 EYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSL 532
              +Y +A IWG KY+  NF RLV +KTKVDP +FF N+QSIP L
Sbjct: 497 SPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of MC03g_new0295 vs. NCBI nr
Match: XP_022152426.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 1096 bits (2835), Expect = 0.0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240
           SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI
Sbjct: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240

Query: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300
           KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL
Sbjct: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300

Query: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360
           SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL
Sbjct: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360

Query: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420
           IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE
Sbjct: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420

Query: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480
           TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL
Sbjct: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480

Query: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540
           GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH
Sbjct: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540

Query: 541 ETS 543
           ETS
Sbjct: 541 ETS 543

BLAST of MC03g_new0295 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 852 bits (2202), Expect = 4.51e-308
Identity = 411/542 (75.83%), Postives = 473/542 (87.27%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPITSLAYIF+    +WVASA N E+F+ CLS+HS N+S I NIIYT TDPSYSS+LN
Sbjct: 1   MKSPITSLAYIFIFL-SAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           +SI N+RF+GAKTPKPQ+IVTPL+ SHIQAS+ICA+ NGFQIRTRSGGHDYEG+SYVSDV
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFVLVD  N+RSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHF
Sbjct: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240
           SGGGYG+MLRK+GLA DNVIDAY +DVNGELHDRKSMGEDVFWAIRGGGASFGIIL+WK+
Sbjct: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240

Query: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300
           KLV VP VVTVF+I RNL++NAT+LVHRWQYVANKQDDRL+IR +L    S NHQNGKR 
Sbjct: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR- 300

Query: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360
           +++A+F+SLFLGKV ELLPIMQ TFPELG+ KEDC EMSWIESVLYF+DF P+ QPL+VL
Sbjct: 301 TVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDF-PREQPLNVL 360

Query: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420
           IDR+ A +G+FKGKSDY+++PIPK GL GLWELL   EV+F RMHL+PYGGKMNEISE++
Sbjct: 361 IDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEAD 420

Query: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480
            P+PHRA NLY IHYVVSW    D  Q+HISWIRKLY YMTPFVSKNPR AY NYRDLD+
Sbjct: 421 IPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDI 480

Query: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540
           G NN +  T+YEKASIWG KYYKDNF+RLVSIKTKVDP++FF N+QSIPSL    K   H
Sbjct: 481 GTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGH 539

Query: 541 ET 542
           ET
Sbjct: 541 ET 539

BLAST of MC03g_new0295 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 823 bits (2127), Expect = 1.17e-296
Identity = 409/543 (75.32%), Postives = 471/543 (86.74%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPITSLAYIFVV  F WVASA N E+F+ CLS HS N SSI  I+YT  D SY SILN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSD- 120
           +SI N RF  AKTPKPQVIVTPLQVSHIQASIICA+ NGFQIRTRSGGHDYEG+SYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGH 180
           VPFV++D  NLRSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+C TVGVGGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 181 FSGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILSW 240
           FSGGGYGVMLRKFGLA DNVIDAY +DVNG L DRKSMGEDVFWAIRGGG ASFGIIL+W
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 241 KIKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGM-KSSNHQNG 300
           KIKL+ VP VVTVFTI RNLE+NAT+L+H+WQYVA+KQDDRL+IR+IL+GM  SSN+QN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 301 KRLSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPL 360
           K+ ++EASF+SLFLG+V+ELLPIMQ+TFPELG+TKEDC EMSWIE+VLYFA F+   QPL
Sbjct: 301 KK-TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQG--QPL 360

Query: 361 DVLIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEIS 420
           +VL+DRS A++G FKGKSDYV+EPIPK G+ G+WEL N++EVQFA+M LIPYGGKM+EIS
Sbjct: 361 NVLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEIS 420

Query: 421 ESETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRD 480
           E E P+PHR GNLY IHY V  G  ED AQ+ ISWIRKLY+YMTPFVSKNPR AY+NYRD
Sbjct: 421 EXEIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 480

Query: 481 LDLGINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKR 540
           LD+G NN +  TSY+KASIWG+KY+K NF+RLVS+KTKVDP++FF N+QSIPSL    K+
Sbjct: 481 LDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKK 540

BLAST of MC03g_new0295 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 823 bits (2126), Expect = 1.54e-296
Identity = 398/531 (74.95%), Postives = 465/531 (87.57%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPI SLAYIFVV  F+ VASA N E+F+HCLS H  N+SSI  IIYT T PSYS +LN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           +SI N RF+GAKTPKPQ+IVTPL+ SH+QASIICA+ NG QIRTRSGGHDYEG+SYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFV+VD  NLRSID+DVE+KVAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHF
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILSWK 240
           SGGGYGVMLRKFGLA DNVIDAY +DVNG+LHDRKSMGEDVFWAIRGGG ASFGI+L+WK
Sbjct: 181 SGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKR 300
           IKL+ VP +VTVFTI RNLE+NAT+L++RWQYVANKQDDRL+I+IIL G  S+NH+NGKR
Sbjct: 241 IKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR 300

Query: 301 LSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDV 360
            +IEA F+SLFLGKVE+LLPIMQ+TFPELG+ KEDC EMSWIESVLYFA+F P+ QPL+V
Sbjct: 301 -TIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEF-PRGQPLNV 360

Query: 361 LIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISES 420
           L+DR   F+G FKGKSDYV+EP+PK GL G+WEL N+KE +  +M LIPYGGKMNEISES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 421 ETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLD 480
           E P+PHRAGNLY IHY +  GGDE+  Q++  WIRK Y+YM PFVSKNPR AY+NYRDLD
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLD 480

Query: 481 LGINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPS 530
           +G NN N  TSY+KASIWG+KY+KDNF+RLVS+K KVDP++FF N+QSIPS
Sbjct: 481 IGTNNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS 527

BLAST of MC03g_new0295 vs. NCBI nr
Match: XP_022152442.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 819 bits (2116), Expect = 5.33e-295
Identity = 393/543 (72.38%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPI+SLAYIFVV  FSWVASAYN E+F+HCLS H  N SSI  IIYT TDP Y S+LN
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           +SI N RF+G KT KPQVIVTPLQ SHIQASIICA+KNGFQIR RSGGHDYEG+SYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFV+VD +NLRSID+DVE++VAWV+SGAT+GELYY+IAEKSKTL +P G+  TVGVGGHF
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240
           SGGGYG ++RKFGLA DNVIDAY +DVNG+LHDR+SMGED+FWAIRGGGASFGIILSWK+
Sbjct: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240

Query: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300
           KLVSVP +VT+F I RNLE+NATDLVH+WQYVANKQDDRL+IRIIL+    +NHQNGKR+
Sbjct: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300

Query: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360
            ++A+F   FLGKVEELLPIMQ TFPELG+ KED  EMSWIES++YFA   PK Q    L
Sbjct: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKL-PKGQA-SAL 360

Query: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420
           +DRS A +G +KGK DYV+EPIPK GL G+WELLN+K++ FA++ LIPYGG+M+EISESE
Sbjct: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420

Query: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480
            P+PHRAGN+Y IHY V++ GD +  Q+HI WIRK YNY+TPFVSKNPR  Y+NYRDLD+
Sbjct: 421 IPFPHRAGNIYKIHYTVAYSGDAE--QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDI 480

Query: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540
           G+NN N  TSYEKAS+WGLKY+K NF+RLV +K KVDPT+FF N+QSIPS L+  K+  H
Sbjct: 481 GMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGH 539

Query: 541 ETS 543
           E S
Sbjct: 541 EMS 539

BLAST of MC03g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1DHP3 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 PE=3 SV=1)

HSP 1 Score: 1096 bits (2835), Expect = 0.0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240
           SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI
Sbjct: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240

Query: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300
           KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL
Sbjct: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300

Query: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360
           SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL
Sbjct: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360

Query: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420
           IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE
Sbjct: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420

Query: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480
           TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL
Sbjct: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480

Query: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540
           GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH
Sbjct: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540

Query: 541 ETS 543
           ETS
Sbjct: 541 ETS 543

BLAST of MC03g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 2.18e-308
Identity = 411/542 (75.83%), Postives = 473/542 (87.27%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPITSLAYIF+    +WVASA N E+F+ CLS+HS N+S I NIIYT TDPSYSS+LN
Sbjct: 1   MKSPITSLAYIFIFL-SAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           +SI N+RF+GAKTPKPQ+IVTPL+ SHIQAS+ICA+ NGFQIRTRSGGHDYEG+SYVSDV
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFVLVD  N+RSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHF
Sbjct: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240
           SGGGYG+MLRK+GLA DNVIDAY +DVNGELHDRKSMGEDVFWAIRGGGASFGIIL+WK+
Sbjct: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240

Query: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300
           KLV VP VVTVF+I RNL++NAT+LVHRWQYVANKQDDRL+IR +L    S NHQNGKR 
Sbjct: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKR- 300

Query: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360
           +++A+F+SLFLGKV ELLPIMQ TFPELG+ KEDC EMSWIESVLYF+DF P+ QPL+VL
Sbjct: 301 TVKANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDF-PREQPLNVL 360

Query: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420
           IDR+ A +G+FKGKSDY+++PIPK GL GLWELL   EV+F RMHL+PYGGKMNEISE++
Sbjct: 361 IDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEAD 420

Query: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480
            P+PHRA NLY IHYVVSW    D  Q+HISWIRKLY YMTPFVSKNPR AY NYRDLD+
Sbjct: 421 IPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDI 480

Query: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540
           G NN +  T+YEKASIWG KYYKDNF+RLVSIKTKVDP++FF N+QSIPSL    K   H
Sbjct: 481 GTNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGH 539

Query: 541 ET 542
           ET
Sbjct: 541 ET 539

BLAST of MC03g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 823 bits (2127), Expect = 5.67e-297
Identity = 409/543 (75.32%), Postives = 471/543 (86.74%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPITSLAYIFVV  F WVASA N E+F+ CLS HS N SSI  I+YT  D SY SILN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSD- 120
           +SI N RF  AKTPKPQVIVTPLQVSHIQASIICA+ NGFQIRTRSGGHDYEG+SYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGH 180
           VPFV++D  NLRSID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+C TVGVGGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 181 FSGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILSW 240
           FSGGGYGVMLRKFGLA DNVIDAY +DVNG L DRKSMGEDVFWAIRGGG ASFGIIL+W
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 241 KIKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGM-KSSNHQNG 300
           KIKL+ VP VVTVFTI RNLE+NAT+L+H+WQYVA+KQDDRL+IR+IL+GM  SSN+QN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 301 KRLSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPL 360
           K+ ++EASF+SLFLG+V+ELLPIMQ+TFPELG+TKEDC EMSWIE+VLYFA F+   QPL
Sbjct: 301 KK-TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQG--QPL 360

Query: 361 DVLIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEIS 420
           +VL+DRS A++G FKGKSDYV+EPIPK G+ G+WEL N++EVQFA+M LIPYGGKM+EIS
Sbjct: 361 NVLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEIS 420

Query: 421 ESETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRD 480
           E E P+PHR GNLY IHY V  G  ED AQ+ ISWIRKLY+YMTPFVSKNPR AY+NYRD
Sbjct: 421 EXEIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 480

Query: 481 LDLGINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKR 540
           LD+G NN +  TSY+KASIWG+KY+K NF+RLVS+KTKVDP++FF N+QSIPSL    K+
Sbjct: 481 LDIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKK 540

BLAST of MC03g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 823 bits (2126), Expect = 7.47e-297
Identity = 398/531 (74.95%), Postives = 465/531 (87.57%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPI SLAYIFVV  F+ VASA N E+F+HCLS H  N+SSI  IIYT T PSYS +LN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           +SI N RF+GAKTPKPQ+IVTPL+ SH+QASIICA+ NG QIRTRSGGHDYEG+SYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFV+VD  NLRSID+DVE+KVAWVQSGAT+GELYY+IAEKSKTLG+P G+CPTVG+GGHF
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGG-ASFGIILSWK 240
           SGGGYGVMLRKFGLA DNVIDAY +DVNG+LHDRKSMGEDVFWAIRGGG ASFGI+L+WK
Sbjct: 181 SGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKR 300
           IKL+ VP +VTVFTI RNLE+NAT+L++RWQYVANKQDDRL+I+IIL G  S+NH+NGKR
Sbjct: 241 IKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR 300

Query: 301 LSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDV 360
            +IEA F+SLFLGKVE+LLPIMQ+TFPELG+ KEDC EMSWIESVLYFA+F P+ QPL+V
Sbjct: 301 -TIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEF-PRGQPLNV 360

Query: 361 LIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISES 420
           L+DR   F+G FKGKSDYV+EP+PK GL G+WEL N+KE +  +M LIPYGGKMNEISES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 421 ETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLD 480
           E P+PHRAGNLY IHY +  GGDE+  Q++  WIRK Y+YM PFVSKNPR AY+NYRDLD
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLD 480

Query: 481 LGINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPS 530
           +G NN N  TSY+KASIWG+KY+KDNF+RLVS+K KVDP++FF N+QSIPS
Sbjct: 481 IGTNNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS 527

BLAST of MC03g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1DG99 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 PE=3 SV=1)

HSP 1 Score: 819 bits (2116), Expect = 2.58e-295
Identity = 393/543 (72.38%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MKSPI+SLAYIFVV  FSWVASAYN E+F+HCLS H  N SSI  IIYT TDP Y S+LN
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120
           +SI N RF+G KT KPQVIVTPLQ SHIQASIICA+KNGFQIR RSGGHDYEG+SYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180
           PFV+VD +NLRSID+DVE++VAWV+SGAT+GELYY+IAEKSKTL +P G+  TVGVGGHF
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHF 180

Query: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240
           SGGGYG ++RKFGLA DNVIDAY +DVNG+LHDR+SMGED+FWAIRGGGASFGIILSWK+
Sbjct: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240

Query: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300
           KLVSVP +VT+F I RNLE+NATDLVH+WQYVANKQDDRL+IRIIL+    +NHQNGKR+
Sbjct: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300

Query: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360
            ++A+F   FLGKVEELLPIMQ TFPELG+ KED  EMSWIES++YFA   PK Q    L
Sbjct: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKL-PKGQA-SAL 360

Query: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420
           +DRS A +G +KGK DYV+EPIPK GL G+WELLN+K++ FA++ LIPYGG+M+EISESE
Sbjct: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420

Query: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480
            P+PHRAGN+Y IHY V++ GD +  Q+HI WIRK YNY+TPFVSKNPR  Y+NYRDLD+
Sbjct: 421 IPFPHRAGNIYKIHYTVAYSGDAE--QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDI 480

Query: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540
           G+NN N  TSYEKAS+WGLKY+K NF+RLV +K KVDPT+FF N+QSIPS L+  K+  H
Sbjct: 481 GMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGH 539

Query: 541 ETS 543
           E S
Sbjct: 541 EMS 539

BLAST of MC03g_new0295 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 557.0 bits (1434), Expect = 1.7e-158
Identity = 264/510 (51.76%), Postives = 372/510 (72.94%), Query Frame = 0

Query: 21  ASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYSIMNERFMGAKTPKPQVIV 80
           A+  NQ +F+ CLSL   +++ +  +I+T  D S+SS+L  SI N+RF     PKP +I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 81  TPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDVPFVLVDFVNLRSIDIDVERK 140
           TP+Q S +Q+++ CAR+ G  IRTRSGGHDYEG+SYV+  PFV++D  NLRSI +DV+ +
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 141 VAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFGLAIDNVI 200
             WVQ+GAT+GELYY+I +K++TL +P GVCPTVGVGGHFSGGGYG +LRK GLA D+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 201 DAYLVDVNGELHDRKSMGEDVFWAIR-GGGASFGIILSWKIKLVSVPSVVTVFTIKRNLE 260
           DA +VD  G + +R+ MGED FWAIR GGG+SF ++LSWKI L++VPS VTVF + +  E
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 261 ENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFLGKVEELLP 320
           ++A  ++HRWQ+VA+K  D L IR++L   K+          + ASF  L+LG V+ LL 
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRVMLQRYKN---------MVRASFPGLYLGSVKNLLK 328

Query: 321 IMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVLIDRSFAFRGVFKGKSDYVD 380
           ++ + FPELG+ ++DCTEMSWIESV++FA  E   +P++VL  R+ A    FK KSD+V 
Sbjct: 329 MVNKEFPELGLEEDDCTEMSWIESVIWFA--ELGEEPINVLTKRTRASL-AFKAKSDFVQ 388

Query: 381 EPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHRAGNLYNIHYVVSW 440
           EP+PKT +  LW  L + E + A++   P+GGKM+EI++ ETP+PHR GN+Y I Y+  W
Sbjct: 389 EPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW 448

Query: 441 GGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGINNINEYTSYEKASIWGL 500
            G  D+ +K++ W+ ++Y+ M+ FV+K+PRGAY+N RDLDLG+    + + YE+   WG+
Sbjct: 449 RG--DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGV 508

Query: 501 KYYKDNFERLVSIKTKVDPTDFFTNQQSIP 530
           KY+K+NFERLV +KT VDP+DFF ++QSIP
Sbjct: 509 KYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MC03g_new0295 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 545.8 bits (1405), Expect = 3.9e-155
Identity = 266/516 (51.55%), Postives = 363/516 (70.35%), Query Frame = 0

Query: 21  ASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYSIMNERFMGAKTPKPQVIV 80
           A   N ENF+ CLS H +N      II+TS DPSY SILN SI N RF   +TPKP  I+
Sbjct: 29  AQRTNHENFLKCLS-HRINEDD-SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 81  TPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVS-DVPFVLVDFVNLRSIDIDVER 140
           TP+Q + +Q++I CAR +G  IRTRSGGHDYEG+SY++   PFV++D  NLRSI +DV+ 
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 141 KVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFSGGGYGVMLRKFGLAIDNV 200
           +  WVQSGAT+GELYY+I + SK+L +P G+ PTVG+GG F GGGYG ++RK+GL+ DNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 201 IDAYLVDVNGELHDRKSMGEDVFWAIR-GGGASFGIILSWKIKLVSVPSVVTVFTIKRNL 260
           IDA++VD NG   DR+ MGED FWAIR GGG+SF ++LSWKI+L+ VPSVVTVF + +  
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 261 EENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRLSIEASFSSLFLGKVEELL 320
           E+ A  ++++WQY+A+K  + L IR +L           K   + ASF  L+LG V +LL
Sbjct: 269 EKEAVSIINKWQYIADKVPNDLFIRAMLQ----------KETEVYASFPGLYLGPVSDLL 328

Query: 321 PIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVLIDRSFAFRGVFKGKSDYV 380
            +M+  FPELG+   +C EMSWIESVL+F     K + +++L  R    R  FKGK D++
Sbjct: 329 ALMKDKFPELGLEIGNCREMSWIESVLWFI----KGESMEILAKRKRTSRS-FKGKDDFI 388

Query: 381 DEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESETPYPHRAGNLYNIHYVVS 440
           +EPIPKT +  LW      E + A++ L P+GGKM+EI+++E P+PHR GNLY I Y+  
Sbjct: 389 EEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAY 448

Query: 441 WGGDED----IAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDLGIN-NINEYTSYEK 500
           W  +ED      +K++ W+  +Y +MTP+VSK+PR AYVN+RD+DLG+   +N  T YE+
Sbjct: 449 WSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEE 508

Query: 501 ASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 530
           A +WG+KY+K+NF+RLV +KT VDP DFF ++QSIP
Sbjct: 509 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0295 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 537.7 bits (1384), Expect = 1.1e-152
Identity = 263/533 (49.34%), Postives = 369/533 (69.23%), Query Frame = 0

Query: 9   AYIFVVSWFSW----VASAY--NQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILNYS 68
           +++F +  FS     + SA+  N E+F+ CLS    + +    +I+TS D S+ SIL+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 69  IMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVS-DVP 128
           I N RF  ++TPKP  I+TP++ S +Q  I CA+ +G  +RTRS GH YEG+SY++ + P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 129 FVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHFS 188
           F ++D  NLRSI +DV+ +  WVQ+GAT GELYY+I + +K+L +P G+ PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 189 GGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIR-GGGASFGIILSWKI 248
           GGGYG +LRK+GLA DN+IDA +VD +G + DR++MGED FWAIR GGG+SFG+ILSWK+
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 249 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 308
           KLV VPS +TVF +++  ++ A  ++ +WQY A+K  D L IR  L        +   + 
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL--------ERSNKN 304

Query: 309 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 368
           ++ A F+ L++G V  LL +M+  FPELG+ KE C EMSWIESVL+FADF PK + L VL
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADF-PKGESLGVL 364

Query: 369 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 428
            +R       FKGK D+V EPIP+  +  +W  L   E +  ++ L P+GGKM+E++E E
Sbjct: 365 TNRERTSLS-FKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYE 424

Query: 429 TPYPHRAGNLYNIHYVVSWGGDED----IAQKHISWIRKLYNYMTPFVSKNPRGAYVNYR 488
           TP+PHR GNLY I YV  W  +ED       K++ W+  +Y +MTP+VSK+PRGAYVN++
Sbjct: 425 TPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFK 484

Query: 489 DLDLGINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIP 530
           D+DLG+    + T YE+   WG+KY+K+NFERLV +KT+VDPTDFF ++QSIP
Sbjct: 485 DMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MC03g_new0295 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 3.3e-146
Identity = 265/540 (49.07%), Postives = 371/540 (68.70%), Query Frame = 0

Query: 7   SLAYIFVVSWF-SWVASAYNQENFIHCLSLHS----LNTSSIL----NIIYTSTDPSYSS 66
           SL  +F+V W  S  +S+ +++ F+ C+S HS    +N  S +    + +YT    S  S
Sbjct: 10  SLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSLIS 69

Query: 67  ILNYSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYV 126
             NY  +   F    + KP +IVTP   + IQ S++C+RK G ++RT+SGGHDYEG+SY+
Sbjct: 70  -QNYRFLTLNF---TSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYL 129

Query: 127 S-DVPFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGV 186
           S   PF+++D VN+RSI+I++  + AWV +GAT+GELYYKIA+ SK  G+P G CP+VGV
Sbjct: 130 SLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGV 189

Query: 187 GGHFSGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHD-RKSMGEDVFWAIRGGG-ASFGI 246
           GGHFSGGG+G M+RK GLA DNV+DA  VD NG +++ R+ MGED+FWAIRGGG ASFG+
Sbjct: 190 GGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGV 249

Query: 247 ILSWKIKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNH 306
           +LSWK+KLV VP  VT F     L +N T +VHRWQ +A + DD L IR+I+S       
Sbjct: 250 VLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG--- 309

Query: 307 QNGKRLSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKR 366
                 S++ +F + +LG +++L+P+M + FPELG+T +DC+EM+WI+S++YF     K 
Sbjct: 310 ------SVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYFN--WKKG 369

Query: 367 QPLDVLIDRSFAFRGV-FKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKM 426
           QPL+ L+DR   +  + FK KSD+V  PIP+ GL G+W   +  EV+   M + P GGKM
Sbjct: 370 QPLETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFH--EVESPIMIMEPLGGKM 429

Query: 427 NEISESETPYPHRAGNLYNIHYVVSWG-GDEDIAQKHISWIRKLYNYMTPFVSKNPRGAY 486
            EI E+ETP+PHR GNLYNI Y+V W   D  + +KH++W+R LY YM  +VS +PRGAY
Sbjct: 430 YEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAY 489

Query: 487 VNYRDLDLGINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLL 533
           +NYRDLDLG+N     TS+E A +WG +Y+  NF+RL  +K K+DPT+FF N+QS+P L+
Sbjct: 490 LNYRDLDLGMNR-GVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPLI 531

BLAST of MC03g_new0295 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 511.5 bits (1316), Expect = 8.1e-145
Identity = 265/535 (49.53%), Postives = 357/535 (66.73%), Query Frame = 0

Query: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60
           MK  +  + +  V  W S  +SA N E F  CL+ +S     I   I+ S + SYSS+L 
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSA-NSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQ 60

Query: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYV--S 120
            +I N RF    TPKP +I+     SH+QA+I C +++  Q++ RSGGHDY+G+SYV  S
Sbjct: 61  ANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYS 120

Query: 121 DVPFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGG 180
             PF ++D  NLRS+D+DV  K AWVQ+GA LGE+YY I EKSKTL YP G+CPTVGVGG
Sbjct: 121 GKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGG 180

Query: 181 HFSGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIR-GGGASFGIILS 240
           H SGGGYG M+RK+GL +DN IDA +VDVNG++ DRK MGED++WAI  GGG S+G++L+
Sbjct: 181 HISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLA 240

Query: 241 WKIKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNG 300
           +KI LV VP  VTVF I R LE+NATD++HRWQ VA K  D L IR ++  +   N    
Sbjct: 241 YKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVV---NGTVS 300

Query: 301 KRLSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPL 360
            + ++  +F ++FLG    LL I+ R FPELG+ + DCTE SWI+SVL++ + +      
Sbjct: 301 SQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSE- 360

Query: 361 DVLIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEIS 420
            +L+ R+       K KSDYV EPI +TGL  +W+     E++   M   PYGG+M  IS
Sbjct: 361 TLLLQRNQPV-NYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRIS 420

Query: 421 ESETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRD 480
            + TP+P+RAGNL+ I Y  +W  DE +  +++   RKLY +MTPFVSKNPR ++ NYRD
Sbjct: 421 STVTPFPYRAGNLWKIQYGANW-RDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRD 480

Query: 481 LDLGINNIN-EYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSL 532
           +DLGIN+ N + +SY +   +G KY+  NFERLV IKT+VD  +FF N+QSIP L
Sbjct: 481 VDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526