MC03g_new0294 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0294 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.1e-151
Identity = 268/524 (51.15%), Postives = 354/524 (67.56%), Query Frame = 0

Query: 11  IFVVLSFSWVASAYN-HEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFSIQNLRFL 70
           IF  LSF    S  N  E+FL C S H PN+ +  K++YT  D LY S+LN +IQNLRF+
Sbjct: 15  IFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFI 74

Query: 71  GGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDVPFVVVDMIN 130
              T KP VIVTP   SHIQA+I+C+KK G QIR RSGGHD EG+SY+S VPFVVVD+ N
Sbjct: 75  SDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRN 134

Query: 131 LRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSLI 190
           + SI +DV  + AWVE+GAT+G++YY I EK++ L+FP G   TVGVGGHFSGGGYG+L+
Sbjct: 135 MHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALM 194

Query: 191 RKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGA-SFGIILSWKVKLVSVPPM 250
           R +GLAADN+IDA+ ++V+GK+ DR+SMGEDLFWAIRGGG  +FGII +WK+KLV+VP  
Sbjct: 195 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSK 254

Query: 251 VTIFKITRNLE-QNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFK 310
            TIF + +N+E      L ++WQ +A K D  L++       N  ++    +  +   F 
Sbjct: 255 STIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFS 314

Query: 311 GFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFA-----KLPKGQASALLDRS 370
             F G V+ L+ +M  +FPELG++K D  E SWI++ I+++          +   LLDRS
Sbjct: 315 SIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRS 374

Query: 371 LALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFP 430
              K  +  K DYV +PIP+  +  I E L E+D+      L PYGG M+EISES IPFP
Sbjct: 375 AGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFP 434

Query: 431 HRAGNIYKIHYTVAYSGDAE-QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNK 490
           HRAG +Y++ YT ++    + ++HI W+R  YN+ TP+VS+NPR  YLNYRDLD+G  N 
Sbjct: 435 HRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNH 494

Query: 491 NGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIP 526
               +Y +A +WG KYF  NF RLV+VK KVDP NFFRNEQSIP
Sbjct: 495 ASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of MC03g_new0294 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.6e-150
Identity = 263/524 (50.19%), Postives = 354/524 (67.56%), Query Frame = 0

Query: 11  IFVVLSFSWVASAYN-HEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFSIQNLRFL 70
           IF  LSF+   S  N  E+FL CLS + P + + +K++YT  D  Y S+LN ++QNLRF 
Sbjct: 15  IFFFLSFNIQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFT 74

Query: 71  GGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDVPFVVVDMIN 130
              T KP VI TPL  SHIQ +I+C+KK G QIR RSGGHD EG+SY+S VPFV+VD+ N
Sbjct: 75  SDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRN 134

Query: 131 LRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSLI 190
           + S+ +DV  + AWVESGAT+G++YY I E ++ L+FPAG   TVG GGHFSGGGYG+L+
Sbjct: 135 MHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALM 194

Query: 191 RKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGA-SFGIILSWKVKLVSVPPM 250
           R +GLAADN+IDA+ ++V+GK+ DR+SMGEDLFWAIRGGG  +FGII +WK++LV+VP M
Sbjct: 195 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSM 254

Query: 251 VTIFKITRNLE-QNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFK 310
            TIF + +N+E      LV++WQ +A   +  L++       N  ++Q   +  + + F 
Sbjct: 255 STIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFS 314

Query: 311 GFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFA-----KLPKGQASALLDRS 370
             F G V+ L+ +M  +FPELG++K D  ++SWI++II+++          +   LLDRS
Sbjct: 315 SIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRS 374

Query: 371 LALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFP 430
              K  +  K DYV +PIP+  +  I E L E+D+        PYGG MDEISES IPFP
Sbjct: 375 GGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFP 434

Query: 431 HRAGNIYKIHYTVAYSGDAE-QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNK 490
           HRAG  Y+I Y  ++    + ++HI WIR  YN+ TP+VS+NPR  YLNYRDLD+G  N 
Sbjct: 435 HRAGITYEIWYIASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 494

Query: 491 NGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIP 526
               +Y +A +WG KYF  NF RLV+VK KVDP NFFRNEQSIP
Sbjct: 495 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of MC03g_new0294 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.8e-150
Identity = 256/508 (50.39%), Postives = 357/508 (70.28%), Query Frame = 0

Query: 21  ASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFSIQNLRFLGGKTSKPQVIV 80
           A+  N   FL CLS    + + +SK+I+TP D  + SVL  SIQN RF      KP +I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 81  TPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDVPFVVVDMINLRSIDVDVEKR 140
           TP+Q S +Q+++ CA++ G  IR RSGGHDYEGLSYV+  PFV++D+ NLRSI VDV+ R
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 141 VAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSLIRKFGLAADNVI 200
             WV++GATIG+LYY I +K++TLAFPAG+  TVGVGGHFSGGGYG+L+RK GLAAD+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 201 DAYFIDVNGKLHDRESMGEDLFWAIR-GGGASFGIILSWKVKLVSVPPMVTIFKITRNLE 260
           DA  +D  G++ +R  MGED FWAIR GGG+SF ++LSWK+ L++VP  VT+F +T+  E
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 261 QNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFKGFFLGKVEELLP 320
           Q+A  ++H+WQ+VA+K  D L IR++L        Q  K +V +A+F G +LG V+ LL 
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMV-RASFPGLYLGSVKNLLK 328

Query: 321 IMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLDRSLALKGTYKGKCDYVNEP 380
           ++   FPELGL ++D  EMSWIES+I+FA+L +   + L  R+ A    +K K D+V EP
Sbjct: 329 MVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPINVLTKRTRA-SLAFKAKSDFVQEP 388

Query: 381 IPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFPHRAGNIYKIHYTVAYSG 440
           +PK  +  +W  L E + + AQL   P+GG+M EI++ E PFPHR GNIY+I Y   + G
Sbjct: 389 MPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG 448

Query: 441 DAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNKNGCTSYEKASVWGLKYFK 500
           D +++++ W+ + Y+ ++ FV+K+PR  Y+N RDLD+GM      + YE+   WG+KYFK
Sbjct: 449 DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFK 508

Query: 501 GNFKRLVRVKIKVDPTNFFRNEQSIPSF 528
            NF+RLVRVK  VDP++FF +EQSIP F
Sbjct: 509 NNFERLVRVKTSVDPSDFFCDEQSIPPF 526

BLAST of MC03g_new0294 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.8e-150
Identity = 260/532 (48.87%), Postives = 366/532 (68.80%), Query Frame = 0

Query: 9   AYIFVVLSFSW----VASAY--NHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFS 68
           +++F +L FS     + SA+  NHEDFL CLS+   +++   K+I+T  D  +FS+L+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 69  IQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVS-DVP 128
           IQN RF   +T KP  I+TP++AS +Q  I CA+ +G  +R RS GH YEGLSY++ + P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 129 FVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFS 188
           F V+D+ NLRSI +DV+ R  WV++GAT G+LYY I + +K+LAFPAG+  TVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 189 GGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIR-GGGASFGIILSWKV 248
           GGGYG+L+RK+GLAADN+IDA  +D +G++ DR++MGED FWAIR GGG+SFG+ILSWKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 249 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 308
           KLV VP  +T+FK+ +  ++ A  ++ +WQY A+K  D L IR  L  +NKN        
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN-------- 304

Query: 309 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD 368
            + A F G ++G V  LL +M+  FPELGL KE   EMSWIES+++FA  PKG++  +L 
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 364

Query: 369 RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP 428
                  ++KGK D+V EPIP+A ++ IW  L   +    ++ L P+GG+M E++E E P
Sbjct: 365 NRERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETP 424

Query: 429 FPHRAGNIYKIHYTVAYSGDAE-------QRHIGWIRKFYNYLTPFVSKNPRATYLNYRD 488
           FPHR GN+Y+I Y VAY  + E        +++ W+   Y ++TP+VSK+PR  Y+N++D
Sbjct: 425 FPHRGGNLYEIQY-VAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 489 LDIGMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIP 526
           +D+GM      T YE+   WG+KYFK NF+RLVRVK +VDPT+FF +EQSIP
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MC03g_new0294 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.8e-150
Identity = 262/524 (50.00%), Postives = 356/524 (67.94%), Query Frame = 0

Query: 11  IFVVLSFSWVASAYN-HEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFSIQNLRFL 70
           IF  LSF+   S  N  E+FL C S + P + + +K++YT  D  Y S+LN +IQNLRF 
Sbjct: 15  IFFFLSFNIQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFT 74

Query: 71  GGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDVPFVVVDMIN 130
              T KP VI+TPL  SHIQ +I+C+KK G QIR RSGGHD EG+SY+S VPFV+VD+ N
Sbjct: 75  SDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRN 134

Query: 131 LRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSLI 190
           + S+ +DV  + AWVE+GAT+G++YY I E ++ L+FPAG   TVG GGHFSGGGYG+L+
Sbjct: 135 MHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALM 194

Query: 191 RKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGA-SFGIILSWKVKLVSVPPM 250
           R +GLAADN+IDA+ ++V+GK+ DR+SMGEDLFWAIRGGG  +FGII +WK++LV+VP M
Sbjct: 195 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSM 254

Query: 251 VTIFKITRNLE-QNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFK 310
            TIF + +N+E      LV++WQ +A   +  L++       N  ++Q   +  + + F 
Sbjct: 255 STIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFS 314

Query: 311 GFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQAS-----ALLDRS 370
             F G V+ L+ +M  +FPELG++K D  ++SWI++II+++ +     +      LLDRS
Sbjct: 315 SIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRS 374

Query: 371 LALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFP 430
              K  +  K DYV +PIP+  +  I E L E+D+        PYGG MDEISES IPFP
Sbjct: 375 GGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFP 434

Query: 431 HRAGNIYKIHYTVAYSGDAE-QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNK 490
           HRAG +Y+I Y  ++    + ++HI WIR  YN+ TP+VS+NPR  YLNYRDLD+G  N 
Sbjct: 435 HRAGIMYEIWYIASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 494

Query: 491 NGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIP 526
               +Y +A +WG KYF  NF RLV+VK KVDP NFFRNEQSIP
Sbjct: 495 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of MC03g_new0294 vs. NCBI nr
Match: XP_022152442.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 1092 bits (2823), Expect = 0.0
Identity = 538/539 (99.81%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFVVVDMINLRSIDVDVEKRVAWVESGATIG+LYYRIAEKSKTLAFPAGLSSTVGVGGHF
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240
           SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV
Sbjct: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240

Query: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300
           KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI
Sbjct: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300

Query: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD 360
           VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD
Sbjct: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD 360

Query: 361 RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP 420
           RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP
Sbjct: 361 RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP 420

Query: 421 FPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNN 480
           FPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNN
Sbjct: 421 FPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNN 480

Query: 481 KNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS 539
           KNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS
Sbjct: 481 KNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS 539

BLAST of MC03g_new0294 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 858 bits (2218), Expect = 1.31e-310
Identity = 418/540 (77.41%), Postives = 475/540 (87.96%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI SLAYIFVVLSF+ VASA N+EDFLHCLSFHFPN SSISKIIYTPT P Y  VLN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           FSIQNLRFLG KT KPQ+IVTPL+ASH+QASIICAK NG QIR RSGGHDYEGLSYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFVVVD+ NLRSIDVDVE++VAWV+SGATIG+LYYRIAEKSKTL FPAGL  TVG+GGHF
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGG-ASFGIILSWK 240
           SGGGYG ++RKFGLAADNVIDAYFIDVNGKLHDR+SMGED+FWAIRGGG ASFGI+L+WK
Sbjct: 181 SGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 VKLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKR 300
           +KL+ VP +VT+F ITRNLEQNAT+L+++WQYVANKQDDRLII+IIL  TN NNH+NGKR
Sbjct: 241 IKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR 300

Query: 301 IVLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQA-SAL 360
            + +A F   FLGKVE+LLPIMQ TFPELGL KED IEMSWIES++YFA+ P+GQ  + L
Sbjct: 301 TI-EAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNVL 360

Query: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420
           LDR    KG++KGK DYVNEP+PKAGLEGIWEL NEK+    Q+ LIPYGG+M+EISESE
Sbjct: 361 LDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESE 420

Query: 421 IPFPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGM 480
           IPFPHRAGN+Y+IHY++   GD EQR+  WIRKFY+Y+ PFVSKNPRA Y+NYRDLDIG 
Sbjct: 421 IPFPHRAGNLYQIHYSIYCGGDEEQRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIGT 480

Query: 481 NNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEM 538
           NN+NG TSY+KAS+WG+KYFK NFKRLV VKIKVDP+NFFRNEQSIPS   WWKK GHE+
Sbjct: 481 NNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS--RWWKKGGHEV 537

BLAST of MC03g_new0294 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 848 bits (2191), Expect = 1.82e-306
Identity = 408/540 (75.56%), Postives = 469/540 (86.85%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI+SLAYIF+ LS +WVASA NHEDFL CLS H PN S IS IIYTPTDP Y SVLN
Sbjct: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           FSIQN RFLG KT KPQ+IVTPL+ASHIQAS+ICAK NGFQIR RSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFV+VD+ N+RSIDVDVE+ VAWV+SGATIG+LYYRIAEKSKTL FPAGL  TVG+GGHF
Sbjct: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240
           SGGGYG ++RK+GLAADNVIDAYFIDVNG+LHDR+SMGED+FWAIRGGGASFGIIL+WKV
Sbjct: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240

Query: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300
           KLV VPP+VT+F ITRNL+QNAT+LVH+WQYVANKQDDRLIIR +L  TN  NHQNGKR 
Sbjct: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRT 300

Query: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQA-SALL 360
           V +A F   FLGKV ELLPIMQNTFPELGL+KED IEMSWIES++YF+  P+ Q  + L+
Sbjct: 301 V-KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLI 360

Query: 361 DRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEI 420
           DR+LALKG +KGK DY+N+PIPKAGLEG+WELL   +++F ++ L+PYGG+M+EISE++I
Sbjct: 361 DRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADI 420

Query: 421 PFPHRAGNIYKIHYTVAYS--GDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIG 480
           PFPHRA N+Y IHY V+++  GD+EQRHI WIRK Y Y+TPFVSKNPRA Y NYRDLDIG
Sbjct: 421 PFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIG 480

Query: 481 MNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHE 537
            NNK+G T+YEKAS+WG KY+K NFKRLV +K KVDP+NFFRNEQSIPS   WWK  GHE
Sbjct: 481 TNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHE 538

BLAST of MC03g_new0294 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 840 bits (2171), Expect = 2.02e-303
Identity = 415/541 (76.71%), Postives = 465/541 (85.95%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI+SLAYIFVVLSF WVASA N+EDFL CLSFH PN+SSISKI+YTP D  YFS+LN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSD- 120
           FSIQNLRF   KT KPQVIVTPLQ SHIQASIICAK NGFQIR RSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGH 180
           VPFVV+D+ NLRSIDVDVEK VAWV+SGATIG+LYYRIAEKSKTL FPAGL STVGVGGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 181 FSGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGG-ASFGIILSW 240
           FSGGGYG ++RKFGLAADNVIDAYFIDVNG L DR+SMGED+FWAIRGGG ASFGIIL+W
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 241 KVKLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNAT-NKNNHQNG 300
           K+KL+ VPP+VT+F ITRNLEQNAT+L+HQWQYVA+KQDDRL+IR+ILN   N +N+QN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 301 KRIVLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASA 360
           K+  L+A+F   FLG+V+ELLPIMQ TFPELGL KED IEMSWIE+++YFA       + 
Sbjct: 301 KK-TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNV 360

Query: 361 LLDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISES 420
           LLDRSLA KGT+KGK DYVNEPIPKAG+EGIWEL NE+++ FAQ+ LIPYGG+M EISE 
Sbjct: 361 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEX 420

Query: 421 EIPFPHRAGNIYKIHYTVAYSG--DAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLD 480
           EIPFPHR GN+YKIHY V      D  QR I WIRK Y+Y+TPFVSKNPRA Y+NYRDLD
Sbjct: 421 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 480

Query: 481 IGMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEG 536
           IG NNK+G TSY+KAS+WG+KYFK NFKRLV VK KVDP+NFFRNEQSIPS   WWKK G
Sbjct: 481 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 540

BLAST of MC03g_new0294 vs. NCBI nr
Match: XP_022152426.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 819 bits (2116), Expect = 5.29e-295
Identity = 392/543 (72.19%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI+SLAYIFVV  FSWVASAYN E+F+HCLS H  N SSI  IIYT TDP Y S+LN
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           +SI N RF+G KT KPQVIVTPLQ SHIQASIICA+KNGFQIR RSGGHDYEG+SYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFV+VD +NLRSID+DVE++VAWV+SGAT+G+LYY+IAEKSKTL +P G+  TVGVGGHF
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240
           SGGGYG ++RKFGLA DNVIDAY +DVNG+LHDR+SMGED+FWAIRGGGASFGIILSWK+
Sbjct: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240

Query: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300
           KLVSVP +VT+F I RNLE+NATDLVH+WQYVANKQDDRL+IRIIL+    +NHQNGKR+
Sbjct: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300

Query: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKL-PKGQA-SAL 360
            ++A+F   FLGKVEELLPIMQ TFPELG+ KED  EMSWIES++YFA   PK Q    L
Sbjct: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360

Query: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420
           +DRS A +G +KGK DYV+EPIPK GL G+WELLN+K++ FA++ LIPYGG+M+EISESE
Sbjct: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420

Query: 421 IPFPHRAGNIYKIHYTVAYSGDAE--QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDI 480
            P+PHRAGN+Y IHY V++ GD +  Q+HI WIRK YNY+TPFVSKNPR  Y+NYRDLD+
Sbjct: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480

Query: 481 GMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGH 539
           G+NN N  TSYEKAS+WGLKY+K NF+RLV +K KVDPT+FF N+QSIPS L+  K+  H
Sbjct: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540

BLAST of MC03g_new0294 vs. ExPASy TrEMBL
Match: A0A6J1DG99 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 PE=3 SV=1)

HSP 1 Score: 1092 bits (2823), Expect = 0.0
Identity = 538/539 (99.81%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFVVVDMINLRSIDVDVEKRVAWVESGATIG+LYYRIAEKSKTLAFPAGLSSTVGVGGHF
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240
           SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV
Sbjct: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240

Query: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300
           KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI
Sbjct: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300

Query: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD 360
           VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD
Sbjct: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD 360

Query: 361 RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP 420
           RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP
Sbjct: 361 RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP 420

Query: 421 FPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNN 480
           FPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNN
Sbjct: 421 FPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNN 480

Query: 481 KNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS 539
           KNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS
Sbjct: 481 KNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEMS 539

BLAST of MC03g_new0294 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 858 bits (2218), Expect = 6.35e-311
Identity = 418/540 (77.41%), Postives = 475/540 (87.96%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI SLAYIFVVLSF+ VASA N+EDFLHCLSFHFPN SSISKIIYTPT P Y  VLN
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           FSIQNLRFLG KT KPQ+IVTPL+ASH+QASIICAK NG QIR RSGGHDYEGLSYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFVVVD+ NLRSIDVDVE++VAWV+SGATIG+LYYRIAEKSKTL FPAGL  TVG+GGHF
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGG-ASFGIILSWK 240
           SGGGYG ++RKFGLAADNVIDAYFIDVNGKLHDR+SMGED+FWAIRGGG ASFGI+L+WK
Sbjct: 181 SGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 VKLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKR 300
           +KL+ VP +VT+F ITRNLEQNAT+L+++WQYVANKQDDRLII+IIL  TN NNH+NGKR
Sbjct: 241 IKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKR 300

Query: 301 IVLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQA-SAL 360
            + +A F   FLGKVE+LLPIMQ TFPELGL KED IEMSWIES++YFA+ P+GQ  + L
Sbjct: 301 TI-EAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNVL 360

Query: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420
           LDR    KG++KGK DYVNEP+PKAGLEGIWEL NEK+    Q+ LIPYGG+M+EISESE
Sbjct: 361 LDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESE 420

Query: 421 IPFPHRAGNIYKIHYTVAYSGDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGM 480
           IPFPHRAGN+Y+IHY++   GD EQR+  WIRKFY+Y+ PFVSKNPRA Y+NYRDLDIG 
Sbjct: 421 IPFPHRAGNLYQIHYSIYCGGDEEQRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIGT 480

Query: 481 NNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHEM 538
           NN+NG TSY+KAS+WG+KYFK NFKRLV VKIKVDP+NFFRNEQSIPS   WWKK GHE+
Sbjct: 481 NNENGNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS--RWWKKGGHEV 537

BLAST of MC03g_new0294 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 848 bits (2191), Expect = 8.81e-307
Identity = 408/540 (75.56%), Postives = 469/540 (86.85%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI+SLAYIF+ LS +WVASA NHEDFL CLS H PN S IS IIYTPTDP Y SVLN
Sbjct: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           FSIQN RFLG KT KPQ+IVTPL+ASHIQAS+ICAK NGFQIR RSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFV+VD+ N+RSIDVDVE+ VAWV+SGATIG+LYYRIAEKSKTL FPAGL  TVG+GGHF
Sbjct: 121 PFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240
           SGGGYG ++RK+GLAADNVIDAYFIDVNG+LHDR+SMGED+FWAIRGGGASFGIIL+WKV
Sbjct: 181 SGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGGGASFGIILAWKV 240

Query: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300
           KLV VPP+VT+F ITRNL+QNAT+LVH+WQYVANKQDDRLIIR +L  TN  NHQNGKR 
Sbjct: 241 KLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGKRT 300

Query: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQA-SALL 360
           V +A F   FLGKV ELLPIMQNTFPELGL+KED IEMSWIES++YF+  P+ Q  + L+
Sbjct: 301 V-KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNVLI 360

Query: 361 DRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEI 420
           DR+LALKG +KGK DY+N+PIPKAGLEG+WELL   +++F ++ L+PYGG+M+EISE++I
Sbjct: 361 DRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEADI 420

Query: 421 PFPHRAGNIYKIHYTVAYS--GDAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIG 480
           PFPHRA N+Y IHY V+++  GD+EQRHI WIRK Y Y+TPFVSKNPRA Y NYRDLDIG
Sbjct: 421 PFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLDIG 480

Query: 481 MNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGHE 537
            NNK+G T+YEKAS+WG KY+K NFKRLV +K KVDP+NFFRNEQSIPS   WWK  GHE
Sbjct: 481 TNNKDGNTNYEKASIWGAKYYKDNFKRLVSIKTKVDPSNFFRNEQSIPSLFPWWKGGGHE 538

BLAST of MC03g_new0294 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 840 bits (2171), Expect = 9.76e-304
Identity = 415/541 (76.71%), Postives = 465/541 (85.95%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI+SLAYIFVVLSF WVASA N+EDFL CLSFH PN+SSISKI+YTP D  YFS+LN
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSD- 120
           FSIQNLRF   KT KPQVIVTPLQ SHIQASIICAK NGFQIR RSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGH 180
           VPFVV+D+ NLRSIDVDVEK VAWV+SGATIG+LYYRIAEKSKTL FPAGL STVGVGGH
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGH 180

Query: 181 FSGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGG-ASFGIILSW 240
           FSGGGYG ++RKFGLAADNVIDAYFIDVNG L DR+SMGED+FWAIRGGG ASFGIIL+W
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAW 240

Query: 241 KVKLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNAT-NKNNHQNG 300
           K+KL+ VPP+VT+F ITRNLEQNAT+L+HQWQYVA+KQDDRL+IR+ILN   N +N+QN 
Sbjct: 241 KIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNX 300

Query: 301 KRIVLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASA 360
           K+  L+A+F   FLG+V+ELLPIMQ TFPELGL KED IEMSWIE+++YFA       + 
Sbjct: 301 KK-TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLNV 360

Query: 361 LLDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISES 420
           LLDRSLA KGT+KGK DYVNEPIPKAG+EGIWEL NE+++ FAQ+ LIPYGG+M EISE 
Sbjct: 361 LLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEX 420

Query: 421 EIPFPHRAGNIYKIHYTVAYSG--DAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLD 480
           EIPFPHR GN+YKIHY V      D  QR I WIRK Y+Y+TPFVSKNPRA Y+NYRDLD
Sbjct: 421 EIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLD 480

Query: 481 IGMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEG 536
           IG NNK+G TSY+KAS+WG+KYFK NFKRLV VK KVDP+NFFRNEQSIPS   WWKK G
Sbjct: 481 IGTNNKDGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWWKKRG 540

BLAST of MC03g_new0294 vs. ExPASy TrEMBL
Match: A0A6J1DHP3 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 PE=3 SV=1)

HSP 1 Score: 819 bits (2116), Expect = 2.56e-295
Identity = 392/543 (72.19%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60
           MKSPI+SLAYIFVV  FSWVASAYN E+F+HCLS H  N SSI  IIYT TDP Y S+LN
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120
           +SI N RF+G KT KPQVIVTPLQ SHIQASIICA+KNGFQIR RSGGHDYEG+SYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHF 180
           PFV+VD +NLRSID+DVE++VAWV+SGAT+G+LYY+IAEKSKTL +P G+  TVGVGGHF
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGHF 180

Query: 181 SGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGGGASFGIILSWKV 240
           SGGGYG ++RKFGLA DNVIDAY +DVNG+LHDR+SMGED+FWAIRGGGASFGIILSWK+
Sbjct: 181 SGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGGGASFGIILSWKI 240

Query: 241 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 300
           KLVSVP +VT+F I RNLE+NATDLVH+WQYVANKQDDRL+IRIIL+    +NHQNGKR+
Sbjct: 241 KLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGKRL 300

Query: 301 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKL-PKGQA-SAL 360
            ++A+F   FLGKVEELLPIMQ TFPELG+ KED  EMSWIES++YFA   PK Q    L
Sbjct: 301 SIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLDVL 360

Query: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420
           +DRS A +G +KGK DYV+EPIPK GL G+WELLN+K++ FA++ LIPYGG+M+EISESE
Sbjct: 361 IDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISESE 420

Query: 421 IPFPHRAGNIYKIHYTVAYSGDAE--QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDI 480
            P+PHRAGN+Y IHY V++ GD +  Q+HI WIRK YNY+TPFVSKNPR  Y+NYRDLD+
Sbjct: 421 TPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDLDL 480

Query: 481 GMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFLTWWKKEGH 539
           G+NN N  TSYEKAS+WGLKY+K NF+RLV +K KVDPT+FF N+QSIPS L+  K+  H
Sbjct: 481 GINNINEYTSYEKASIWGLKYYKDNFERLVSIKTKVDPTDFFTNQQSIPSLLSSKKRREH 540

BLAST of MC03g_new0294 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 532.7 bits (1371), Expect = 3.4e-151
Identity = 256/508 (50.39%), Postives = 357/508 (70.28%), Query Frame = 0

Query: 21  ASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFSIQNLRFLGGKTSKPQVIV 80
           A+  N   FL CLS    + + +SK+I+TP D  + SVL  SIQN RF      KP +I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 81  TPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDVPFVVVDMINLRSIDVDVEKR 140
           TP+Q S +Q+++ CA++ G  IR RSGGHDYEGLSYV+  PFV++D+ NLRSI VDV+ R
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 141 VAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSLIRKFGLAADNVI 200
             WV++GATIG+LYY I +K++TLAFPAG+  TVGVGGHFSGGGYG+L+RK GLAAD+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 201 DAYFIDVNGKLHDRESMGEDLFWAIR-GGGASFGIILSWKVKLVSVPPMVTIFKITRNLE 260
           DA  +D  G++ +R  MGED FWAIR GGG+SF ++LSWK+ L++VP  VT+F +T+  E
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 261 QNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFKGFFLGKVEELLP 320
           Q+A  ++H+WQ+VA+K  D L IR++L        Q  K +V +A+F G +LG V+ LL 
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMV-RASFPGLYLGSVKNLLK 328

Query: 321 IMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLDRSLALKGTYKGKCDYVNEP 380
           ++   FPELGL ++D  EMSWIES+I+FA+L +   + L  R+ A    +K K D+V EP
Sbjct: 329 MVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPINVLTKRTRA-SLAFKAKSDFVQEP 388

Query: 381 IPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFPHRAGNIYKIHYTVAYSG 440
           +PK  +  +W  L E + + AQL   P+GG+M EI++ E PFPHR GNIY+I Y   + G
Sbjct: 389 MPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG 448

Query: 441 DAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNKNGCTSYEKASVWGLKYFK 500
           D +++++ W+ + Y+ ++ FV+K+PR  Y+N RDLD+GM      + YE+   WG+KYFK
Sbjct: 449 DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFK 508

Query: 501 GNFKRLVRVKIKVDPTNFFRNEQSIPSF 528
            NF+RLVRVK  VDP++FF +EQSIP F
Sbjct: 509 NNFERLVRVKTSVDPSDFFCDEQSIPPF 526

BLAST of MC03g_new0294 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 532.7 bits (1371), Expect = 3.4e-151
Identity = 260/532 (48.87%), Postives = 366/532 (68.80%), Query Frame = 0

Query: 9   AYIFVVLSFSW----VASAY--NHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFS 68
           +++F +L FS     + SA+  NHEDFL CLS+   +++   K+I+T  D  +FS+L+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 69  IQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVS-DVP 128
           IQN RF   +T KP  I+TP++AS +Q  I CA+ +G  +R RS GH YEGLSY++ + P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 129 FVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFS 188
           F V+D+ NLRSI +DV+ R  WV++GAT G+LYY I + +K+LAFPAG+  TVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 189 GGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIR-GGGASFGIILSWKV 248
           GGGYG+L+RK+GLAADN+IDA  +D +G++ DR++MGED FWAIR GGG+SFG+ILSWKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 249 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 308
           KLV VP  +T+FK+ +  ++ A  ++ +WQY A+K  D L IR  L  +NKN        
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN-------- 304

Query: 309 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLD 368
            + A F G ++G V  LL +M+  FPELGL KE   EMSWIES+++FA  PKG++  +L 
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 364

Query: 369 RSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIP 428
                  ++KGK D+V EPIP+A ++ IW  L   +    ++ L P+GG+M E++E E P
Sbjct: 365 NRERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETP 424

Query: 429 FPHRAGNIYKIHYTVAYSGDAE-------QRHIGWIRKFYNYLTPFVSKNPRATYLNYRD 488
           FPHR GN+Y+I Y VAY  + E        +++ W+   Y ++TP+VSK+PR  Y+N++D
Sbjct: 425 FPHRGGNLYEIQY-VAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 489 LDIGMNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIP 526
           +D+GM      T YE+   WG+KYFK NF+RLVRVK +VDPT+FF +EQSIP
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MC03g_new0294 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 528.5 bits (1360), Expect = 6.4e-150
Identity = 256/514 (49.81%), Postives = 358/514 (69.65%), Query Frame = 0

Query: 21  ASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLNFSIQNLRFLGGKTSKPQVIV 80
           A   NHE+FL CLS     D   S+II+T  DP YFS+LN SIQN RF   +T KP  I+
Sbjct: 29  AQRTNHENFLKCLSHRINEDD--SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 81  TPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVS-DVPFVVVDMINLRSIDVDVEK 140
           TP+QA+ +Q++I CA+ +G  IR RSGGHDYEGLSY++   PFVV+D+ NLRSI +DV+ 
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 141 RVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSLIRKFGLAADNV 200
           R  WV+SGATIG+LYY I + SK+LAFPAGL  TVG+GG F GGGYG+L+RK+GL+ADNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 201 IDAYFIDVNGKLHDRESMGEDLFWAIR-GGGASFGIILSWKVKLVSVPPMVTIFKITRNL 260
           IDA+ +D NG   DR+ MGED FWAIR GGG+SF ++LSWK++L+ VP +VT+FK+ +  
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 261 EQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFKGFFLGKVEELL 320
           E+ A  ++++WQY+A+K  + L IR +L           K   + A+F G +LG V +LL
Sbjct: 269 EKEAVSIINKWQYIADKVPNDLFIRAMLQ----------KETEVYASFPGLYLGPVSDLL 328

Query: 321 PIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASALLDRSLALKGTYKGKCDYVNE 380
            +M++ FPELGL   +  EMSWIES+++F    KG++  +L +      ++KGK D++ E
Sbjct: 329 ALMKDKFPELGLEIGNCREMSWIESVLWFI---KGESMEILAKRKRTSRSFKGKDDFIEE 388

Query: 381 PIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFPHRAGNIYKIHYTVAYS 440
           PIPK  ++ +W      +   A++ L P+GG+M EI+++EIPFPHR GN+Y+I Y   +S
Sbjct: 389 PIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWS 448

Query: 441 GDAE------QRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMN-NKNGCTSYEKAS 500
            + +      ++++ W+   Y ++TP+VSK+PR  Y+N+RD+D+GM    N  T YE+A 
Sbjct: 449 EEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAK 508

Query: 501 VWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIP 526
           VWG+KYFK NF RLVRVK  VDP +FF +EQSIP
Sbjct: 509 VWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0294 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 511.9 bits (1317), Expect = 6.2e-145
Identity = 260/531 (48.96%), Postives = 355/531 (66.85%), Query Frame = 0

Query: 8   LAYIFVVLSFSWVASAYNHEDFLHCL----SFHFPNDSSISKIIYTPTDPLYFSVLNFSI 67
           L+  ++ LSFS +      +DF+ CL       FP DSS     +      +   L  S 
Sbjct: 19  LSLSYIPLSFSTI-----QQDFVMCLVDNSDASFPMDSSF--FTHDLNASSFKLALETSA 78

Query: 68  QNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV--P 127
           QNLR+L     KP+ I  PL  +H+QA+++CAKK    +R+RSGGHDYEGLSYVS++   
Sbjct: 79  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 138

Query: 128 FVVVDMINLRSIDVDVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFS 187
           FV+VD+  LR I VD+E   AWV +GA+IG++YYRI EKSK   FPAGL +++G+GGH  
Sbjct: 139 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 198

Query: 188 GGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIR-GGGASFGIILSWKV 247
           GG YGS++RKFGL ADNV+DA  +D +GK+ +R +MGED+FWAIR GGG SFG+IL+WK+
Sbjct: 199 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 258

Query: 248 KLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRI 307
           KLV VP +VT+F +TR LEQ+ T L+++WQ VA+K D+ L IR+I+  T+K      K  
Sbjct: 259 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKT--PKSKER 318

Query: 308 VLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPK-GQASALL 367
            +  +++G FLG    LL +MQ +FP+LGL K+D +E SWI+S++Y A  P    + ALL
Sbjct: 319 TISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALL 378

Query: 368 DRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEI 427
           D     K  +K K DYV EPIP  GLEG+WE L E+D      +  PYGG M +I E+E 
Sbjct: 379 DGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWN--PYGGMMAKIPETET 438

Query: 428 PFPHRAGNIYKIHYTVAYSG--DAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIG 487
           PFPHR+G ++KI +   +     +E +H+GW+R+ Y+Y+  +VSK+PR+ Y+NYRDLD+G
Sbjct: 439 PFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLG 498

Query: 488 MNNKNGCTSYEKASVWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPSFL 529
           MN K        A  WG +YFKGNF+RLV +K K DP NFFR+EQSIP+ L
Sbjct: 499 MNGKG-----SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of MC03g_new0294 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 502.3 bits (1292), Expect = 4.9e-142
Identity = 248/515 (48.16%), Postives = 345/515 (66.99%), Query Frame = 0

Query: 20  VASAYNHEDFLHCLSFHFPNDSSISKIIYTPTD--PLYFSVLNFSIQNLRFLGGKTSKPQ 79
           ++S+   +DF+ CL  +      I+   ++P     L+   L  + QNLR+L     KP 
Sbjct: 24  LSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPV 83

Query: 80  VIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVS--DVPFVVVDMINLRSIDV 139
            I  PL  +H+QA+++CAKK    +R+RSGGHDYEGLS+V+  + PFV+VD+  LR +DV
Sbjct: 84  FIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDV 143

Query: 140 DVEKRVAWVESGATIGKLYYRIAEKSKTLAFPAGLSSTVGVGGHFSGGGYGSLIRKFGLA 199
           D++   AW  +GATIG++YYRI EKS+T  FPAGL S++G+GGH  GG YGS++RKFGL 
Sbjct: 144 DLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLG 203

Query: 200 ADNVIDAYFIDVNGKLHDRESMGEDLFWAIR-GGGASFGIILSWKVKLVSVPPMVTIFKI 259
           ADNV+DA  +D NG++ DR +MGED+FWAIR GGG SFG+IL+WK+KLV VP  VT+F +
Sbjct: 204 ADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTV 263

Query: 260 TRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGKRIVLQAAFKGFFLGKV 319
           T+ LEQ+ T ++++W+ +A+K DD L IR+I++  +K      + I +  +++  FLG  
Sbjct: 264 TKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISM--SYQAQFLGDS 323

Query: 320 EELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQA-SALLDRSLALKGTYKGKC 379
             LL +MQ +FPELGL K+D  EMSWI+S++Y A  P   A  ALL      K  +K K 
Sbjct: 324 NRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSLFKNHFKAKS 383

Query: 380 DYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESEIPFPHRAGNIYKIHY 439
           D+V EPIP  GLEG+WE   E+D      +  PYGG M  ISESEIPFPHR G ++KI +
Sbjct: 384 DFVKEPIPVEGLEGLWERFLEEDSPLTIWN--PYGGMMSRISESEIPFPHRNGTLFKIQW 443

Query: 440 TVAYSGD--AEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDIGMNNKNGCTSYEKAS 499
              +     +E+RH+ WIR+ Y+Y+  +VSKNPR  Y+NYRDLD+G N          A 
Sbjct: 444 LSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE-----GETDAR 503

Query: 500 VWGLKYFKGNFKRLVRVKIKVDPTNFFRNEQSIPS 527
            WG KY+KGNF+RLV++K + DP NFFR+EQS+P+
Sbjct: 504 EWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529