MC03g_new0289 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0289 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.1e-118
Identity = 221/454 (48.68%), Postives = 304/454 (66.96%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWAH--KHEVTLPCISDHSQDSYPISKVVYSPMDSTY 60
           M++SS L  +   L F L  S    AH   HE  L C+S    D+    KV+++  DS++
Sbjct: 1   MEFSSFLFTI---LLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSF 60

Query: 61  FSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLS 120
           FS+L+ SI+NPRFS  +TPKP+ IITP   S +Q  + C++ HG+ +R RS GH YEGLS
Sbjct: 61  FSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLS 120

Query: 121 YISH-LPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTV 180
           YI++  PF ++DL NLRSI++DV + T WVQ+GAT GE YY I + +++LAFPAG+ PTV
Sbjct: 121 YIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTV 180

Query: 181 GVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFG 240
           GVGG FSGGGYG ++RKYGLAADN+IDA ++DA+G+  DR++MGED FWAIRGG G SFG
Sbjct: 181 GVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFG 240

Query: 241 IVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNV 300
           ++++WKVKLV VP+T+T   + +T +K+AV++I +WQY A K+ ++LF+ T L       
Sbjct: 241 VILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLE------ 300

Query: 301 SQGGGETNKNPI-ALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKL 360
                 +NKN + ALF  L++G V+ L+ +++  FPEL L KE C E SWIES L  A  
Sbjct: 301 -----RSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFA-- 360

Query: 361 TGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMP 420
             FP  ESL  L  R+  T  S K K D+V+EPI E AI+ IW RL+  + +    I  P
Sbjct: 361 -DFPKGESLGVLTNRE-RTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTP 420

Query: 421 YGGIMSQIPESETPYAHRARYLYKIGYIIQWEEK 451
           +GG MS++ E ETP+ HR   LY+I Y+  W E+
Sbjct: 421 FGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREE 436

BLAST of MC03g_new0289 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 9.1e-118
Identity = 216/433 (49.88%), Postives = 289/433 (66.74%), Query Frame = 0

Query: 26  AHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFSVLNFSIRNPRFSTIDTPKPLLIITP 85
           A+  E  L C S H  ++    K+VY+  D  Y S+LN +I+N RF +  TPKPL+I+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 86  ANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYISHLPFIIVDLINLRSITIDVKHETA 145
           +N SHIQA ++CSK  GL+IR RSGGHD EG+SYIS +PF++VDL N+ SI IDV  +TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 146 WVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGGYGLMMRKYGLAADNVIDA 205
           WV++GATLGE YY I EK+  L+FP G CPTVGVGGHFSGGGYG +MR YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 206 YLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVVAWKVKLVPVPATVTSCSINRTLE-K 265
           +L++ +GK  DR+SMGEDLFWAIRGG G +FGI+ AWK+KLV VP+  T  S+ + +E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 266 DAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQGGGETNKNPIALFFTLFLGKVDELV 325
             VKL ++WQ +A+K D++L L T       N++   G+        F ++F G VD LV
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLVLMTHFI--TKNITDNHGKNKTTVHGYFSSIFHGGVDSLV 327

Query: 326 KILKTNFPELVLAKEDCIESSWIESALVNAKLTGF-PADESLETLLKRDPLTKASMKIKS 385
            ++  +FPEL + K DC E SWI++ +  + +  F  A+   E LL R    K +  IK 
Sbjct: 328 DLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKL 387

Query: 386 DYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGIMSQIPESETPYAHRARYLYKIGY 445
           DYVK+PI ETA+  I E+L  +D    +++  PYGGIM +I ES  P+ HRA  +Y++ Y
Sbjct: 388 DYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWY 447

Query: 446 IIQWEEKGVDAEK 457
              W EK  D EK
Sbjct: 448 TASW-EKQEDNEK 457

BLAST of MC03g_new0289 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.6e-117
Identity = 221/451 (49.00%), Postives = 311/451 (68.96%), Query Frame = 0

Query: 3   YSSPLIPLSFSLAFVLLS-SSPTWAHKHEVT--LPCISDHSQDSYPISKVVYSPMDSTYF 62
           +SS LI   F+ + +LLS   P  A++   +  L C+S    DS  +SKV+++P D+++ 
Sbjct: 7   FSSVLI--FFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFS 66

Query: 63  SVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSY 122
           SVL  SI+N RFS  D PKP+LI+TP   S +Q+AV C++  G+ IR RSGGHDYEGLSY
Sbjct: 67  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 126

Query: 123 ISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGV 182
           ++H PF+I+DL NLRSIT+DV + + WVQ+GAT+GE YY I +K+RTLAFPAG+CPTVGV
Sbjct: 127 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 186

Query: 183 GGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIV 242
           GGHFSGGGYG ++RK+GLAAD+VIDA ++DA G+  +R  MGED FWAIRGG G SF +V
Sbjct: 187 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 246

Query: 243 VAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQ 302
           ++WK+ L+ VP+TVT  ++ +  E+ A+K+IHRWQ+VA K+ ++LF+  +L         
Sbjct: 247 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML--------- 306

Query: 303 GGGETNKNPI-ALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTG 362
              +  KN + A F  L+LG V  L+K++   FPEL L ++DC E SWIES +  A+L  
Sbjct: 307 ---QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-- 366

Query: 363 FPADESLETLLKRDPLTKASM--KIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMP 422
              +E +  L KR   T+AS+  K KSD+V+EP+ +TAI  +W RL+  + +    IF P
Sbjct: 367 --GEEPINVLTKR---TRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTP 426

Query: 423 YGGIMSQIPESETPYAHRARYLYKIGYIIQW 448
           +GG MS+I + ETP+ HR   +Y+I Y+  W
Sbjct: 427 FGGKMSEIADYETPFPHRKGNIYEIQYLNYW 436

BLAST of MC03g_new0289 vs. ExPASy Swiss-Prot
Match: A0A2S1XB67 (O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 3.5e-117
Identity = 214/454 (47.14%), Postives = 310/454 (68.28%), Query Frame = 0

Query: 8   IPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFSVLNFSIR 67
           +P+  S+   LL  S +     E  L CIS+       I  +++ P +S+Y SVL  +I+
Sbjct: 5   VPIVLSIFCFLLLLSSSHGSIPEAFLNCISNKFSLDVSILNILHVPSNSSYDSVLKSTIQ 64

Query: 68  NPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYISHLPFII 127
           NPRF  + +PKPL IITP   SH+Q+AVIC+K  GL+IRIRSGG DYEGLSY S +PFI+
Sbjct: 65  NPRF--LKSPKPLAIITPVLHSHVQSAVICTKQAGLQIRIRSGGADYEGLSYRSEVPFIL 124

Query: 128 VDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGG 187
           +DL NLRSI++D++  +AWV+SGAT+GEFY+ IA+ S   AFPAG+  +VG+GGH S GG
Sbjct: 125 LDLQNLRSISVDIEDNSAWVESGATIGEFYHEIAQNSPVHAFPAGVSSSVGIGGHLSSGG 184

Query: 188 YGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVVAWKVKLV 247
           +G ++RKYGLAADN+IDA ++DA G+  DRESMGEDLFWAIRGG G SFG++V+WKVKLV
Sbjct: 185 FGTLLRKYGLAADNIIDAKIVDARGRILDRESMGEDLFWAIRGGGGASFGVIVSWKVKLV 244

Query: 248 PVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQGGGETNKN 307
            VP  VT   +++T E+  + L+H+WQY+ HKL E+LFL       +++ S  G   NK 
Sbjct: 245 KVPPMVTVFILSKTYEEGGLDLLHKWQYIEHKLPEDLFLAV----SIMDDSSSG---NKT 304

Query: 308 PIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFPADESLET 367
            +A F +LFLGK ++L+K++  NFP+L L KEDC+E +WI++A+     +G P  ES   
Sbjct: 305 LMAGFMSLFLGKTEDLLKVMAENFPQLGLKKEDCLEMNWIDAAMY---FSGHPIGESRSV 364

Query: 368 LLKRDP-LTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGIMSQIPE 427
           L  R+  L K  + IKSD+++EP S  A+E +W+  + +++   + + +P GG+MS+I E
Sbjct: 365 LKNRESHLPKTCVSIKSDFIQEPQSMDALEKLWKFCR-EEENSPIILMLPLGGMMSKISE 424

Query: 428 SETPYAHRARYLYKIGYIIQWEEKGVDAEKIHLD 461
           SE P+ +R   +Y + Y I W  +  ++ + ++D
Sbjct: 425 SEIPFPYRKDVIYSMIYEIVWNCEDDESSEEYID 445

BLAST of MC03g_new0289 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 5.9e-117
Identity = 218/437 (49.89%), Postives = 291/437 (66.59%), Query Frame = 0

Query: 26  AHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFSVLNFSIRNPRFSTIDTPKPLLIITP 85
           A+  E  L C S +   +   +K+VY+  D  Y S+LN +I+N RF++  TPKPL+IITP
Sbjct: 28  ANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITP 87

Query: 86  ANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYISHLPFIIVDLINLRSITIDVKHETA 145
            N+SHIQ  ++CSK  GL+IR RSGGHD EG+SYIS +PF+IVDL N+ S+ IDV  +TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 146 WVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGGYGLMMRKYGLAADNVIDA 205
           WV++GATLGE YY I E +  L+FPAG CPTVG GGHFSGGGYG +MR YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 206 YLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVVAWKVKLVPVPATVTSCSINRTLE-K 265
           +L++ +GK  DR+SMGEDLFWAIRGG G +FGI+ AWK++LV VP+  T  S+ + +E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 266 DAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQGGGETNKNPIALFFTLFLGKVDELV 325
           + VKL+++WQ +A+  ++ L L T       N++   G+      + F ++F G VD LV
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFI--TRNITDNQGKNKTTIHSYFSSIFHGGVDSLV 327

Query: 326 KILKTNFPELVLAKEDCIESSWIE-----SALVNAKLTGFPADESLETLLKRDPLTKASM 385
            ++  +FPEL + K DC + SWI+     S +VN   T F      E LL R    KA+ 
Sbjct: 328 DLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYF----KKEILLDRSGGRKAAF 387

Query: 386 KIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGIMSQIPESETPYAHRARYLY 445
            IK DYVK+PI ETA+  I E+L  +D    +F+F PYGGIM +I ES  P+ HRA  +Y
Sbjct: 388 SIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMY 447

Query: 446 KIGYIIQWEEKGVDAEK 457
           +I YI  W EK  D EK
Sbjct: 448 EIWYIASW-EKQEDNEK 457

BLAST of MC03g_new0289 vs. NCBI nr
Match: XP_022152424.1 (berberine bridge enzyme-like 28 [Momordica charantia])

HSP 1 Score: 930 bits (2403), Expect = 0.0
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFS 60
           MKYSSPLIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFS
Sbjct: 1   MKYSSPLIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFS 60

Query: 61  VLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYI 120
           VLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYI
Sbjct: 61  VLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYI 120

Query: 121 SHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVG 180
           SHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVG
Sbjct: 121 SHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVG 180

Query: 181 GHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVV 240
           GHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVV
Sbjct: 181 GHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVV 240

Query: 241 AWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQG 300
           AWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQG
Sbjct: 241 AWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQG 300

Query: 301 GGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFP 360
           GGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFP
Sbjct: 301 GGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFP 360

Query: 361 ADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGI 420
           ADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGI
Sbjct: 361 ADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGI 420

Query: 421 MSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHLD 460
           MSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHLD
Sbjct: 421 MSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHLD 460

BLAST of MC03g_new0289 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 676 bits (1743), Expect = 1.28e-239
Identity = 339/464 (73.06%), Postives = 384/464 (82.76%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWA----HKHEVTLPCISDHSQDS-YPISKVVYSPMD 60
           MKYSSPLIPLSFSL F+ LSSS  WA     KHE  L C+ DHS DS Y ISKVVY+P++
Sbjct: 1   MKYSSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLN 60

Query: 61  STYFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYE 120
           S+Y SVL+FSIRN RFST DTPKPL IITP ++SH+QAAV+CS+THGL+IR RSGGHD+E
Sbjct: 61  SSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFE 120

Query: 121 GLSYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCP 180
           GLS +SHLPFII+DLINLRSITID  + TAWVQSGATLGE YY IAEKSRTLAFPAG CP
Sbjct: 121 GLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACP 180

Query: 181 TVGVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGS 240
           TVG+GGHFSGGGYGLM+RK+GLAADNVIDAYL+DANGKF DRE MGEDLFWAIRGG GGS
Sbjct: 181 TVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGS 240

Query: 241 FGIVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVL 300
           FGIVVAWKVKLVPVPATVTSC  NRTLE+ A+KLIHRWQYVA+KLDENLFLG +L GG  
Sbjct: 241 FGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNF 300

Query: 301 NVSQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAK 360
             +QGG +TN  P A FF+LFLGK DE   IL T FPEL LAKE+C+ESSWIESAL++A 
Sbjct: 301 T-AQGGDKTN--PTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA- 360

Query: 361 LTGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFM 420
             GF   +SLE LL R PLT  S+KIKSDYVKEPISE  IEGIWERLK++D   +  +F+
Sbjct: 361 --GFKIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFV 420

Query: 421 PYGGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           PYGG MSQI ESE P++HRA  LYKIGYI+ W+++ VD EK HL
Sbjct: 421 PYGGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHL 458

BLAST of MC03g_new0289 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 666 bits (1718), Expect = 7.24e-236
Identity = 331/465 (71.18%), Postives = 390/465 (83.87%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWA----HKHEVTLPCISDHSQD--SYPISKVVYSPM 60
           M Y SPLI  S SLAF+ LSSS  WA    +KHE  L C+ DHS +  +Y ISKV+++P+
Sbjct: 1   MNYPSPLI--SLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPI 60

Query: 61  DSTYFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDY 120
           +S+Y SVL+FSIRN RFST++TPKPLLI+TP+++SHIQAAVICSK++G +IR RSGGHD+
Sbjct: 61  NSSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDF 120

Query: 121 EGLSYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLC 180
           EGLSY++HLPFIIVDLINLRSI+ID+K  TAWVQSGATLGE YY IA+KSRTLAFPAG+C
Sbjct: 121 EGLSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGIC 180

Query: 181 PTVGVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGG 240
           PTVGVGGHFSGGGYGL++RKYGLAADNVIDAYL+DA+GKFH+RESMGEDLFWAIRGG GG
Sbjct: 181 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGG 240

Query: 241 SFGIVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGV 300
           SFGIVVAWKVKLVPVPATVT CS NRTLE+ AVK+ HRWQYVA+KLDENLFLG +  GG 
Sbjct: 241 SFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGK 300

Query: 301 LNVSQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNA 360
           +  SQ GG+TN  P+A FF+LFLGK DEL+ IL T FPEL LAK+DCIE+SWIES ++  
Sbjct: 301 IT-SQEGGKTN--PVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG 360

Query: 361 KLTGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIF 420
              GFP + +LE LL R PLT  S KIKSDYVKEP+SE  ++GIW+RL +QD +G   IF
Sbjct: 361 --VGFPTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIF 420

Query: 421 MPYGGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           +PYGG MSQI ESETP++HRA YLYKIGYI  WEE+G+DAEK HL
Sbjct: 421 VPYGGRMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHL 458

BLAST of MC03g_new0289 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 649 bits (1675), Expect = 2.31e-229
Identity = 325/463 (70.19%), Postives = 382/463 (82.51%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWA----HKHEVTLPCISDHSQDSYPISKVVYSPMDS 60
           MKYS PL  LS  LAF+LLSSS +WA     KHE  L C+S HS DS  ISKV+Y+P++ 
Sbjct: 1   MKYS-PLTILS--LAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINP 60

Query: 61  TYFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEG 120
           +Y SVLNFS+RN RFS+ +TPKPLLIITP+ +SH+QAAVICSKTHGL+IRIRSGGHD EG
Sbjct: 61  SYSSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEG 120

Query: 121 LSYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPT 180
           LSY++HLPFII+DLINL+SIT+++++ TAW+QSGATLGE YY IA+KSRTLAFPAG+CPT
Sbjct: 121 LSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSF 240
           VGVGGHFSGGGYGLM+RKYGLAADNVIDAYLIDANGKF+DRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLN 300
           GIVVAWKVKLVPVPA VT+CS +R L++D + LIHRWQ VA+KLDENL+ G IL+GG  N
Sbjct: 241 GIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGG--N 300

Query: 301 VSQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKL 360
            S  GG    NP A FF+LFLGK DEL+ IL TNFPEL LAK+DC+E+SWIES LV A  
Sbjct: 301 ASTEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAA-- 360

Query: 361 TGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMP 420
           T     ESLE+LL R PLT  + K+KSDYVKEPI +  IEGIWERLK QD +    +F+P
Sbjct: 361 TRNQTIESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVP 420

Query: 421 YGGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           YGG MS+I +SETP++HRAR LYKIGYI+ W+E+ V AE+ HL
Sbjct: 421 YGGRMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHL 456

BLAST of MC03g_new0289 vs. NCBI nr
Match: XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])

HSP 1 Score: 648 bits (1672), Expect = 6.60e-229
Identity = 325/463 (70.19%), Postives = 381/463 (82.29%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWA----HKHEVTLPCISDHSQDSYPISKVVYSPMDS 60
           MKY+SPLI LS  L  +++SSSP WA     K++  L C+ +HS D+YPISKVVYSP++S
Sbjct: 1   MKYTSPLILLS--LVLIVVSSSPLWASASEQKYQHFLQCLLEHSLDNYPISKVVYSPINS 60

Query: 61  TYFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEG 120
           +Y SVL+FSIRN RFS   TPKPLLIITP+++SHIQAAVICSK+HGL+IR RSGGHD+EG
Sbjct: 61  SYSSVLDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 120

Query: 121 LSYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPT 180
           LSY+++LPFIIVDLINLRS+TIDV++ TAWVQSGATLGE YYRI EKSRTLAFPAG+CPT
Sbjct: 121 LSYVAYLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSF 240
           VGVGGHFSGGGYG M+RK+GLAADNVIDAYL+DANGK HDRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLN 300
           GIVVAWKVKLVPVPATVTSCS +R LE DA+KL+H+WQYVA+KLDENLFLG +L+GG + 
Sbjct: 241 GIVVAWKVKLVPVPATVTSCSTHRNLEGDAIKLVHQWQYVANKLDENLFLGILLTGGNIT 300

Query: 301 VSQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKL 360
            +Q G     +P A FF LFLGKVDELV  L T FPEL L K+DC+E+SWIES L+ A  
Sbjct: 301 TTQEGIA---DPTATFFILFLGKVDELVATLSTTFPELSLMKQDCVETSWIESTLIAA-- 360

Query: 361 TGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMP 420
           TG    ESLE LL R P T  S KIKSDY+KEPI E  IEGI++RLK QD      IF+P
Sbjct: 361 TGVQIVESLEPLLNRTPPTIESTKIKSDYIKEPIPEATIEGIFQRLKAQDIATSQLIFVP 420

Query: 421 YGGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           YGG MSQI ESETP++HRA  LYKIGYI+ W+E+ ++A+K H+
Sbjct: 421 YGGRMSQISESETPFSHRAANLYKIGYIVGWKEQSLNAKKRHI 456

BLAST of MC03g_new0289 vs. ExPASy TrEMBL
Match: A0A6J1DFZ4 (berberine bridge enzyme-like 28 OS=Momordica charantia OX=3673 GN=LOC111020160 PE=4 SV=1)

HSP 1 Score: 930 bits (2403), Expect = 0.0
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFS 60
           MKYSSPLIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFS
Sbjct: 1   MKYSSPLIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFS 60

Query: 61  VLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYI 120
           VLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYI
Sbjct: 61  VLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYI 120

Query: 121 SHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVG 180
           SHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVG
Sbjct: 121 SHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVG 180

Query: 181 GHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVV 240
           GHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVV
Sbjct: 181 GHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVV 240

Query: 241 AWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQG 300
           AWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQG
Sbjct: 241 AWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQG 300

Query: 301 GGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFP 360
           GGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFP
Sbjct: 301 GGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFP 360

Query: 361 ADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGI 420
           ADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGI
Sbjct: 361 ADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGI 420

Query: 421 MSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHLD 460
           MSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHLD
Sbjct: 421 MSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHLD 460

BLAST of MC03g_new0289 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 676 bits (1743), Expect = 6.18e-240
Identity = 339/464 (73.06%), Postives = 384/464 (82.76%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWA----HKHEVTLPCISDHSQDS-YPISKVVYSPMD 60
           MKYSSPLIPLSFSL F+ LSSS  WA     KHE  L C+ DHS DS Y ISKVVY+P++
Sbjct: 1   MKYSSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLN 60

Query: 61  STYFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYE 120
           S+Y SVL+FSIRN RFST DTPKPL IITP ++SH+QAAV+CS+THGL+IR RSGGHD+E
Sbjct: 61  SSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFE 120

Query: 121 GLSYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCP 180
           GLS +SHLPFII+DLINLRSITID  + TAWVQSGATLGE YY IAEKSRTLAFPAG CP
Sbjct: 121 GLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACP 180

Query: 181 TVGVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGS 240
           TVG+GGHFSGGGYGLM+RK+GLAADNVIDAYL+DANGKF DRE MGEDLFWAIRGG GGS
Sbjct: 181 TVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGS 240

Query: 241 FGIVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVL 300
           FGIVVAWKVKLVPVPATVTSC  NRTLE+ A+KLIHRWQYVA+KLDENLFLG +L GG  
Sbjct: 241 FGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNF 300

Query: 301 NVSQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAK 360
             +QGG +TN  P A FF+LFLGK DE   IL T FPEL LAKE+C+ESSWIESAL++A 
Sbjct: 301 T-AQGGDKTN--PTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA- 360

Query: 361 LTGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFM 420
             GF   +SLE LL R PLT  S+KIKSDYVKEPISE  IEGIWERLK++D   +  +F+
Sbjct: 361 --GFKIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFV 420

Query: 421 PYGGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           PYGG MSQI ESE P++HRA  LYKIGYI+ W+++ VD EK HL
Sbjct: 421 PYGGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHL 458

BLAST of MC03g_new0289 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 666 bits (1718), Expect = 3.50e-236
Identity = 331/465 (71.18%), Postives = 390/465 (83.87%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWA----HKHEVTLPCISDHSQD--SYPISKVVYSPM 60
           M Y SPLI  S SLAF+ LSSS  WA    +KHE  L C+ DHS +  +Y ISKV+++P+
Sbjct: 1   MNYPSPLI--SLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPI 60

Query: 61  DSTYFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDY 120
           +S+Y SVL+FSIRN RFST++TPKPLLI+TP+++SHIQAAVICSK++G +IR RSGGHD+
Sbjct: 61  NSSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDF 120

Query: 121 EGLSYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLC 180
           EGLSY++HLPFIIVDLINLRSI+ID+K  TAWVQSGATLGE YY IA+KSRTLAFPAG+C
Sbjct: 121 EGLSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGIC 180

Query: 181 PTVGVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGG 240
           PTVGVGGHFSGGGYGL++RKYGLAADNVIDAYL+DA+GKFH+RESMGEDLFWAIRGG GG
Sbjct: 181 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGG 240

Query: 241 SFGIVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGV 300
           SFGIVVAWKVKLVPVPATVT CS NRTLE+ AVK+ HRWQYVA+KLDENLFLG +  GG 
Sbjct: 241 SFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGK 300

Query: 301 LNVSQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNA 360
           +  SQ GG+TN  P+A FF+LFLGK DEL+ IL T FPEL LAK+DCIE+SWIES ++  
Sbjct: 301 IT-SQEGGKTN--PVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG 360

Query: 361 KLTGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIF 420
              GFP + +LE LL R PLT  S KIKSDYVKEP+SE  ++GIW+RL +QD +G   IF
Sbjct: 361 --VGFPTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIF 420

Query: 421 MPYGGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           +PYGG MSQI ESETP++HRA YLYKIGYI  WEE+G+DAEK HL
Sbjct: 421 VPYGGRMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHL 458

BLAST of MC03g_new0289 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 649 bits (1675), Expect = 1.12e-229
Identity = 325/463 (70.19%), Postives = 382/463 (82.51%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWA----HKHEVTLPCISDHSQDSYPISKVVYSPMDS 60
           MKYS PL  LS  LAF+LLSSS +WA     KHE  L C+S HS DS  ISKV+Y+P++ 
Sbjct: 1   MKYS-PLTILS--LAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINP 60

Query: 61  TYFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEG 120
           +Y SVLNFS+RN RFS+ +TPKPLLIITP+ +SH+QAAVICSKTHGL+IRIRSGGHD EG
Sbjct: 61  SYSSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEG 120

Query: 121 LSYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPT 180
           LSY++HLPFII+DLINL+SIT+++++ TAW+QSGATLGE YY IA+KSRTLAFPAG+CPT
Sbjct: 121 LSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSF 240
           VGVGGHFSGGGYGLM+RKYGLAADNVIDAYLIDANGKF+DRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLN 300
           GIVVAWKVKLVPVPA VT+CS +R L++D + LIHRWQ VA+KLDENL+ G IL+GG  N
Sbjct: 241 GIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGG--N 300

Query: 301 VSQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKL 360
            S  GG    NP A FF+LFLGK DEL+ IL TNFPEL LAK+DC+E+SWIES LV A  
Sbjct: 301 ASTEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAA-- 360

Query: 361 TGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMP 420
           T     ESLE+LL R PLT  + K+KSDYVKEPI +  IEGIWERLK QD +    +F+P
Sbjct: 361 TRNQTIESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVP 420

Query: 421 YGGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           YGG MS+I +SETP++HRAR LYKIGYI+ W+E+ V AE+ HL
Sbjct: 421 YGGRMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHL 456

BLAST of MC03g_new0289 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 637 bits (1643), Expect = 2.20e-224
Identity = 324/462 (70.13%), Postives = 375/462 (81.17%), Query Frame = 0

Query: 3   YSSPLIPLSFSLAFVLLSSSPTW----AHKHEVTLPCISDHSQD-SYPISKVVYSPMDST 62
           YSSPLIPL   LAF+ LSSS  W    A KHE  L C+ +HS D +Y ISKV+++P++S+
Sbjct: 5   YSSPLIPLF--LAFIFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSS 64

Query: 63  YFSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGL 122
           Y SVL+FSIRN RFST +TPKPLLIITP+++SHIQAAV+CSK +G +IR RSGGHD+E L
Sbjct: 65  YSSVLDFSIRNLRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERL 124

Query: 123 SYISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTV 182
           SY++HL FIIVDLINLRSI++DVK+  AWV SGATLGE YY IA+KSRT AFPAG+CPTV
Sbjct: 125 SYVAHLSFIIVDLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTV 184

Query: 183 GVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFG 242
           GVGGHFSGGGYGL++RKYGLAADNVIDAYL+DANGKFHDRESMGEDLFWAIRGG GGSFG
Sbjct: 185 GVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 244

Query: 243 IVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNV 302
           IVVAWKVKLVPVPATVT C  NRTLE+ AVKL HRWQYVA+KLDENLFLG +  GG +  
Sbjct: 245 IVVAWKVKLVPVPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGNVT- 304

Query: 303 SQGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLT 362
           SQGGG+TN  P+A FF+LFLG+ DEL+ IL T FPEL LAK+DCIE+SWIES ++     
Sbjct: 305 SQGGGKTN--PVATFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMG--I 364

Query: 363 GFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPY 422
           GF    +LE LL R PLT  S KIKSDYVKEPISE  I+GI ERL  QD +    IF+PY
Sbjct: 365 GFQTKVTLEALLSRTPLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFVPY 424

Query: 423 GGIMSQIPESETPYAHRARYLYKIGYIIQWEEKGVDAEKIHL 459
           GG MSQI ESETP++HRA YLYKIGYI  W ++ +D EK HL
Sbjct: 425 GGRMSQIFESETPFSHRAGYLYKIGYITSWLDQSIDTEKRHL 459

BLAST of MC03g_new0289 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 426.4 bits (1095), Expect = 2.9e-119
Identity = 221/454 (48.68%), Postives = 304/454 (66.96%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWAH--KHEVTLPCISDHSQDSYPISKVVYSPMDSTY 60
           M++SS L  +   L F L  S    AH   HE  L C+S    D+    KV+++  DS++
Sbjct: 1   MEFSSFLFTI---LLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSF 60

Query: 61  FSVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLS 120
           FS+L+ SI+NPRFS  +TPKP+ IITP   S +Q  + C++ HG+ +R RS GH YEGLS
Sbjct: 61  FSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLS 120

Query: 121 YISH-LPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTV 180
           YI++  PF ++DL NLRSI++DV + T WVQ+GAT GE YY I + +++LAFPAG+ PTV
Sbjct: 121 YIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTV 180

Query: 181 GVGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFG 240
           GVGG FSGGGYG ++RKYGLAADN+IDA ++DA+G+  DR++MGED FWAIRGG G SFG
Sbjct: 181 GVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFG 240

Query: 241 IVVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNV 300
           ++++WKVKLV VP+T+T   + +T +K+AV++I +WQY A K+ ++LF+ T L       
Sbjct: 241 VILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLE------ 300

Query: 301 SQGGGETNKNPI-ALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKL 360
                 +NKN + ALF  L++G V+ L+ +++  FPEL L KE C E SWIES L  A  
Sbjct: 301 -----RSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFA-- 360

Query: 361 TGFPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMP 420
             FP  ESL  L  R+  T  S K K D+V+EPI E AI+ IW RL+  + +    I  P
Sbjct: 361 -DFPKGESLGVLTNRE-RTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTP 420

Query: 421 YGGIMSQIPESETPYAHRARYLYKIGYIIQWEEK 451
           +GG MS++ E ETP+ HR   LY+I Y+  W E+
Sbjct: 421 FGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREE 436

BLAST of MC03g_new0289 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-118
Identity = 221/451 (49.00%), Postives = 311/451 (68.96%), Query Frame = 0

Query: 3   YSSPLIPLSFSLAFVLLS-SSPTWAHKHEVT--LPCISDHSQDSYPISKVVYSPMDSTYF 62
           +SS LI   F+ + +LLS   P  A++   +  L C+S    DS  +SKV+++P D+++ 
Sbjct: 7   FSSVLI--FFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFS 66

Query: 63  SVLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSY 122
           SVL  SI+N RFS  D PKP+LI+TP   S +Q+AV C++  G+ IR RSGGHDYEGLSY
Sbjct: 67  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 126

Query: 123 ISHLPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGV 182
           ++H PF+I+DL NLRSIT+DV + + WVQ+GAT+GE YY I +K+RTLAFPAG+CPTVGV
Sbjct: 127 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 186

Query: 183 GGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIV 242
           GGHFSGGGYG ++RK+GLAAD+VIDA ++DA G+  +R  MGED FWAIRGG G SF +V
Sbjct: 187 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 246

Query: 243 VAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQ 302
           ++WK+ L+ VP+TVT  ++ +  E+ A+K+IHRWQ+VA K+ ++LF+  +L         
Sbjct: 247 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML--------- 306

Query: 303 GGGETNKNPI-ALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTG 362
              +  KN + A F  L+LG V  L+K++   FPEL L ++DC E SWIES +  A+L  
Sbjct: 307 ---QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-- 366

Query: 363 FPADESLETLLKRDPLTKASM--KIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMP 422
              +E +  L KR   T+AS+  K KSD+V+EP+ +TAI  +W RL+  + +    IF P
Sbjct: 367 --GEEPINVLTKR---TRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTP 426

Query: 423 YGGIMSQIPESETPYAHRARYLYKIGYIIQW 448
           +GG MS+I + ETP+ HR   +Y+I Y+  W
Sbjct: 427 FGGKMSEIADYETPFPHRKGNIYEIQYLNYW 436

BLAST of MC03g_new0289 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 414.5 bits (1064), Expect = 1.1e-115
Identity = 219/452 (48.45%), Postives = 292/452 (64.60%), Query Frame = 0

Query: 1   MKYSSPLIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMDSTYFS 60
           MKY+  LI + F + F+  SSS   +   E    C++ +S   +PIS  ++   + +Y S
Sbjct: 1   MKYA--LILVLFFVVFIWQSSSS--SANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSS 60

Query: 61  VLNFSIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYI 120
           VL  +IRN RF+T  TPKP LII   + SH+QAA+ C K H L+++IRSGGHDY+GLSY+
Sbjct: 61  VLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYV 120

Query: 121 SH--LPFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVG 180
           ++   PF ++D+ NLRS+ +DV  +TAWVQ+GA LGE YY I EKS+TLA+PAG+CPTVG
Sbjct: 121 TYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVG 180

Query: 181 VGGHFSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGI 240
           VGGH SGGGYG MMRKYGL  DN IDA ++D NGK  DR+ MGEDL+WAI GG GGS+G+
Sbjct: 181 VGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGV 240

Query: 241 VVAWKVKLVPVPATVTSCSINRTLEKDAVKLIHRWQYVAHKLDENLFLGTILSGGVLNVS 300
           V+A+K+ LV VP  VT   I+RTLE++A  +IHRWQ VA KL + LF+ T     V++V 
Sbjct: 241 VLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRT-----VIDVV 300

Query: 301 QGGGETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTG 360
            G   + K     F  +FLG    L+ IL   FPEL L + DC E+SWI+S L    +  
Sbjct: 301 NGTVSSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNI-- 360

Query: 361 FPADESLETLLKRDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYG 420
                S ETLL +       +K KSDYV+EPIS T +E IW+  K  + +     F PYG
Sbjct: 361 --QVGSSETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYG 420

Query: 421 GIMSQIPESETPYAHRARYLYKIGYIIQWEEK 451
           G M +I  + TP+ +RA  L+KI Y   W ++
Sbjct: 421 GEMGRISSTVTPFPYRAGNLWKIQYGANWRDE 437

BLAST of MC03g_new0289 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 412.5 bits (1059), Expect = 4.3e-115
Identity = 203/423 (47.99%), Postives = 284/423 (67.14%), Query Frame = 0

Query: 29  HEVTLPCISDHSQDSYPISKVVYSPMDSTYFSVLNFSIRNPRFSTIDTPKPLLIITPANI 88
           HE  L C+S    +    S+++++  D +YFS+LN SI+NPRF  ++TPKP+ IITP   
Sbjct: 34  HENFLKCLSHRINED--DSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 93

Query: 89  SHIQAAVICSKTHGLEIRIRSGGHDYEGLSYIS-HLPFIIVDLINLRSITIDVKHETAWV 148
           + +Q+ + C++ HG+ IR RSGGHDYEGLSY++   PF+++DL NLRSIT+DV + T WV
Sbjct: 94  TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 153

Query: 149 QSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGGYGLMMRKYGLAADNVIDAYL 208
           QSGAT+GE YY I + S++LAFPAGL PTVG+GG F GGGYG +MRKYGL+ADNVIDA++
Sbjct: 154 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 213

Query: 209 IDANGKFHDRESMGEDLFWAIRGGAGGSFGIVVAWKVKLVPVPATVTSCSINRTLEKDAV 268
           +DANG F DR+ MGED FWAIRGG G SF +V++WK++L+ VP+ VT   + +T EK+AV
Sbjct: 214 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 273

Query: 269 KLIHRWQYVAHKLDENLFLGTILSGGVLNVSQGGGETNKNPIALFFTLFLGKVDELVKIL 328
            +I++WQY+A K+  +LF+  +L            +      A F  L+LG V +L+ ++
Sbjct: 274 SIINKWQYIADKVPNDLFIRAML------------QKETEVYASFPGLYLGPVSDLLALM 333

Query: 329 KTNFPELVLAKEDCIESSWIESALVNAKLTGFPADESLETLLKRDPLTKASMKIKSDYVK 388
           K  FPEL L   +C E SWIES L       F   ES+E L KR   T  S K K D+++
Sbjct: 334 KDKFPELGLEIGNCREMSWIESVL------WFIKGESMEILAKR-KRTSRSFKGKDDFIE 393

Query: 389 EPISETAIEGIWERLKTQDKQGKVFIFMPYGGIMSQIPESETPYAHRARYLYKIGYIIQW 448
           EPI +TAI+ +W R +  + +    I  P+GG MS+I ++E P+ HR   LY+I Y+  W
Sbjct: 394 EPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYW 435

Query: 449 EEK 451
            E+
Sbjct: 454 SEE 435

BLAST of MC03g_new0289 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 406.4 bits (1043), Expect = 3.1e-113
Identity = 211/447 (47.20%), Postives = 297/447 (66.44%), Query Frame = 0

Query: 7   LIPLSFSLAFVLLSSSPTWAHKHEVTLPCISDHSQDSYPISKVVYSPMD--STYFSVLNF 66
           ++ L FSL  +  +SS   A   +  + C+  ++   +P+ K  ++P    S +  VL  
Sbjct: 12  ILVLYFSLYTITPTSS--LASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLES 71

Query: 67  SIRNPRFSTIDTPKPLLIITPANISHIQAAVICSKTHGLEIRIRSGGHDYEGLSYISHL- 126
           + +N RF     PKP  I +P + SH+QA++ICSK   + +R+RSGGHDYEGLSY+S + 
Sbjct: 72  TAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQID 131

Query: 127 -PFIIVDLINLRSITIDVKHETAWVQSGATLGEFYYRIAEKSRTLAFPAGLCPTVGVGGH 186
            PFI++DL  +R + I+++  +AWVQSGAT+GE YYRIAEKS+   FPAGLC ++G+GGH
Sbjct: 132 KPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGH 191

Query: 187 FSGGGYGLMMRKYGLAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGAGGSFGIVVAW 246
            +GG YG MMRKYGL ADNV+DA ++DANGK  DR +MGED FWAIRGGAGGSFGI++AW
Sbjct: 192 ITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAW 251

Query: 247 KVKLVPVPATVTSCSINRTLEKD-AVKLIHRWQYVAHKLDENLFLGTILSGGVLNVSQGG 306
           K+KLVPVP TVT  ++ +TL++D   K+I +WQ VA KL E LF+  +      NV+  G
Sbjct: 252 KIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVL-----FNVAGTG 311

Query: 307 GETNKNPIALFFTLFLGKVDELVKILKTNFPELVLAKEDCIESSWIESALVNAKLTGFPA 366
           G  NK     +  LFLG    L+ ++K +FPEL L  +DCIE SW+ES    A ++GFP 
Sbjct: 312 G--NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESI---AYISGFPT 371

Query: 367 DESLETLLK-RDPLTKASMKIKSDYVKEPISETAIEGIWERLKTQDKQGKVFIFMPYGGI 426
                 LL+ + P  K S K KSD+VK PI E+ ++GI+++L  +D    + I+ PYGG+
Sbjct: 372 HTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGM 431

Query: 427 MSQIPESETPYAHRARYLYKIGYIIQW 448
           M++IPES+ P+ HR   L+K+ Y+  W
Sbjct: 432 MAKIPESQIPFPHRKGVLFKVQYVTSW 444